CmaCh01G010170 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh01G010170
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial
Location	Cma_Chr01: 7463516 .. 7466238 (-)
RNA-Seq Expression	CmaCh01G010170
Synteny	CmaCh01G010170

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GGGGGGGGGTGGAAGAGGCAGTCCCCACCACGCCACCACCACCTTCCAAACAAAATCAAACCCAACCCAAAAACTGACACCTCTATTTATGCACATCCACTCTTTCACATCGTTTTCATCTCTTCCTCTTCTCTCTCTCAATTTTATTCTCTTTCTTTTCCCATTCCCTCACTATGATTCGGACTTCCCTCGGCCTTCTCAGTCGGGATTTTCCTGGCGGCGGCGGCGGCGCCCGTCAGAATGCCTCGGTGTTACTTTCCTCTTCTAACCGCTCCGCTTTGCTTTCGCCGACACGTCGTTTCGAATCCCCCGAAGCCGCGCGACTCCATGAAGACGAAAACGATGCTCAGGTTGGTGGCTTTATCATTTTTCTATCTCTTTATTTAAATATTAATAATAATCTATATTTGTTTTATAAAAATTATTTTCTAAAATAAAATGTTTTTTTTTCTTTTCATAATTATGAAAGGAAAATAATTTAATGTTTTTTTTTTCTTTTATTTGGGAAAAAAAAAGTCAAATCTGAAATTTCTTTACCATTTATAATGATCTTAAAATTTACTTACCATCCTTGTGTATATTATATAATATAATACAATAATGAGATAACTTTTAGTATTTAATATTAAAAATTTATCAAATAATATAATATCATTTATGTTAACTATTTCAATTAAAACATTTGATTATTAACTTTTTAAACTTTTAATTAAAATATATATATATATATATATATATATATATATATATATATATATATATATATTTACTGTGGTAGGAGTTGGATTTTCCGGGAGGAAGAGTTCCATTTACGAGTGAAATGAAATTCATTGCTGAGTCGAGTGAGAAGCGGGTGTCGTGTTTTCGAATTCTCCACGAAAATGGGGAGACGATTAAGCCCACCAATTTCAAGCAGGTTTTACTTTCTTTTTTTTTTTCTTTTTTTTTTTTTTAATAATAATATAATTTATATATAGTTGAGCAAGGATGTATCGATGAAGATGTACAAAGACATGATTACCCTTCAAACCATGGACACCATATTCTTCGAAGCACAAAGGCAAGGAAGAATCTCATTTTACTTAACTTCAGCCGGAGAAGAAGCAGTGACCATTGCTTCAGCAGCTGCGCTTCACTCCGACGACGTGGTTTTGGCTCAGGTACGTATGTATGTATGTATGTATGTACCTTAATTTAGCTTATAATATAATCTCGTTTTGGTTTAAGAGAGTCGGGTTGATTTATTACAGTACAGGGAGCCGGGAGTGTTGTTATGGCGTGGATTTGGATTGCAAGAATTTGCAGATCAGGTGTTTGGAAACAGAAGTGATTATGGAAAAGGTAGGCAGATGCCCATTCACTATGGCTCCAATCTGCTCAATTTCCTCACCATTTCATCGCCAATTGGGTAACACAACGCGCCTTCATCCCTCAATACTTCATACTTAACTTTGTGGCTATCAAATTGAAACAATGTGTGTCCTTTATTAACTGTCTGTTGCAGAACGCAGCTTCCACATGCTGTTGGCGTTGCTTATTCTCTTAAAATGGACAGAAAAGACGCCTGCGCTGTGACTTATTTTGGAGATGGCGGCACCAGCGAGGTAGATACCTTAAATATCCTCTTCTCTTTTTCCAATCTTGAACACTTAATAATGTTAGATGAACACGAGTCTTCACGATTGTATGATATTGTCTACTTTGAGCATAAGTTCTCGTGACTTTGTTTTGAGCTTTCCCAAAAGGGCTCATACGAATGAAGATAGTATTCTTTGATTATAAACCCATGATCATTCCCTAAATTAGTCGATGTGTGGGACTTTCAGCCTCCAACACATGGAACTTTAATTGAATTTTGTTACTTACACAGGGGGATTTCCACGCGGCATTGAACTTTGCAGCAGTGTTAGGAGCGCCGGTGGTTTTCATCTGCCGGAACAACGGCTGGGCTATCAGTACTTCCATCCAAGAACAATTCCGAAGTACCAAATCCATTCTAAAATCTCACACCTCTCAATCCCAACCTCTGATTCCCTTTTTTTGTTTCCATTTTCAGGCGACGGGGTGGTGGTTAAAGGTCAGGCCTATGGAGTCCGAAGCATTAGGATTGATGGCAATGATGCTCTGGCTGTTTATAGCGCCACTCGCAGAGCGAGAGAGATGGCGGTTGCCGAGCAGATGCCGGTTCTGATCGAGGCTCTAACTTACAGAGTTGGCCACCATTCCACCTCCGATGATTCCACCAAGTATCGGGGTGTGGATGAGATTGAGTATTGGAAAACAAAGAGGAGCCCTATGGATCGGTTTGCAAGATGGTTAAGAAATAATGGATGGTTGAGTGAAGAAGATGAGTCCATGCATCGATCCACTGTAAAAAAACAGGTCAGATCCTTTGTTTTTAAATACACCTTCACCTCTTATACCACTGTTTCAAGCTCTTTTCCAATACAAAATCGTTATCAAACAAGATCCGGTTGAAACTAAGATCAAGACATAACTGGATTACAGTAGAGTTCTTGTTGAAACTAAAAATAATCAAATTGTTGGTTTTTTGGTTGTTGCAAGCAATCCAGAAAGCAGAGAAGGCAGAGAAAGCTCCAGTTTCAGCATTGTTCAGTGATGTCTATGATCACATACCCTCAAACCTTCATGAGCAAGAACAGGTACTGAGGCAAACAATGAAGAGGTATCCCCAAGATTACCCTTCTGATGTTCCTCTTTAG

mRNA sequence

GGGGGGGGGTGGAAGAGGCAGTCCCCACCACGCCACCACCACCTTCCAAACAAAATCAAACCCAACCCAAAAACTGACACCTCTATTTATGCACATCCACTCTTTCACATCGTTTTCATCTCTTCCTCTTCTCTCTCTCAATTTTATTCTCTTTCTTTTCCCATTCCCTCACTATGATTCGGACTTCCCTCGGCCTTCTCAGTCGGGATTTTCCTGGCGGCGGCGGCGGCGCCCGTCAGAATGCCTCGGTGTTACTTTCCTCTTCTAACCGCTCCGCTTTGCTTTCGCCGACACGTCGTTTCGAATCCCCCGAAGCCGCGCGACTCCATGAAGACGAAAACGATGCTCAGGAGTTGGATTTTCCGGGAGGAAGAGTTCCATTTACGAGTGAAATGAAATTCATTGCTGAGTCGAGTGAGAAGCGGGTGTCGTGTTTTCGAATTCTCCACGAAAATGGGGAGACGATTAAGCCCACCAATTTCAAGCAGTTGAGCAAGGATGTATCGATGAAGATGTACAAAGACATGATTACCCTTCAAACCATGGACACCATATTCTTCGAAGCACAAAGGCAAGGAAGAATCTCATTTTACTTAACTTCAGCCGGAGAAGAAGCAGTGACCATTGCTTCAGCAGCTGCGCTTCACTCCGACGACGTGGTTTTGGCTCAGTACAGGGAGCCGGGAGTGTTGTTATGGCGTGGATTTGGATTGCAAGAATTTGCAGATCAGGTGTTTGGAAACAGAAGTGATTATGGAAAAGGTAGGCAGATGCCCATTCACTATGGCTCCAATCTGCTCAATTTCCTCACCATTTCATCGCCAATTGGAACGCAGCTTCCACATGCTGTTGGCGTTGCTTATTCTCTTAAAATGGACAGAAAAGACGCCTGCGCTGTGACTTATTTTGGAGATGGCGGCACCAGCGAGGGGGATTTCCACGCGGCATTGAACTTTGCAGCAGTGTTAGGAGCGCCGGTGGTTTTCATCTGCCGGAACAACGGCTGGGCTATCAGTACTTCCATCCAAGAACAATTCCGAAGCGACGGGGTGGTGGTTAAAGGTCAGGCCTATGGAGTCCGAAGCATTAGGATTGATGGCAATGATGCTCTGGCTGTTTATAGCGCCACTCGCAGAGCGAGAGAGATGGCGGTTGCCGAGCAGATGCCGGTTCTGATCGAGGCTCTAACTTACAGAGTTGGCCACCATTCCACCTCCGATGATTCCACCAAGTATCGGGGTGTGGATGAGATTGAGTATTGGAAAACAAAGAGGAGCCCTATGGATCGGTTTGCAAGATGGTTAAGAAATAATGGATGGTTGAGTGAAGAAGATGAGTCCATGCATCGATCCACTGTAAAAAAACAGAAAGCAGAGAAGGCAGAGAAAGCTCCAGTTTCAGCATTGTTCAGTGATGTCTATGATCACATACCCTCAAACCTTCATGAGCAAGAACAGGTACTGAGGCAAACAATGAAGAGGTATCCCCAAGATTACCCTTCTGATGTTCCTCTTTAG

Coding sequence (CDS)

ATGATTCGGACTTCCCTCGGCCTTCTCAGTCGGGATTTTCCTGGCGGCGGCGGCGGCGCCCGTCAGAATGCCTCGGTGTTACTTTCCTCTTCTAACCGCTCCGCTTTGCTTTCGCCGACACGTCGTTTCGAATCCCCCGAAGCCGCGCGACTCCATGAAGACGAAAACGATGCTCAGGAGTTGGATTTTCCGGGAGGAAGAGTTCCATTTACGAGTGAAATGAAATTCATTGCTGAGTCGAGTGAGAAGCGGGTGTCGTGTTTTCGAATTCTCCACGAAAATGGGGAGACGATTAAGCCCACCAATTTCAAGCAGTTGAGCAAGGATGTATCGATGAAGATGTACAAAGACATGATTACCCTTCAAACCATGGACACCATATTCTTCGAAGCACAAAGGCAAGGAAGAATCTCATTTTACTTAACTTCAGCCGGAGAAGAAGCAGTGACCATTGCTTCAGCAGCTGCGCTTCACTCCGACGACGTGGTTTTGGCTCAGTACAGGGAGCCGGGAGTGTTGTTATGGCGTGGATTTGGATTGCAAGAATTTGCAGATCAGGTGTTTGGAAACAGAAGTGATTATGGAAAAGGTAGGCAGATGCCCATTCACTATGGCTCCAATCTGCTCAATTTCCTCACCATTTCATCGCCAATTGGAACGCAGCTTCCACATGCTGTTGGCGTTGCTTATTCTCTTAAAATGGACAGAAAAGACGCCTGCGCTGTGACTTATTTTGGAGATGGCGGCACCAGCGAGGGGGATTTCCACGCGGCATTGAACTTTGCAGCAGTGTTAGGAGCGCCGGTGGTTTTCATCTGCCGGAACAACGGCTGGGCTATCAGTACTTCCATCCAAGAACAATTCCGAAGCGACGGGGTGGTGGTTAAAGGTCAGGCCTATGGAGTCCGAAGCATTAGGATTGATGGCAATGATGCTCTGGCTGTTTATAGCGCCACTCGCAGAGCGAGAGAGATGGCGGTTGCCGAGCAGATGCCGGTTCTGATCGAGGCTCTAACTTACAGAGTTGGCCACCATTCCACCTCCGATGATTCCACCAAGTATCGGGGTGTGGATGAGATTGAGTATTGGAAAACAAAGAGGAGCCCTATGGATCGGTTTGCAAGATGGTTAAGAAATAATGGATGGTTGAGTGAAGAAGATGAGTCCATGCATCGATCCACTGTAAAAAAACAGAAAGCAGAGAAGGCAGAGAAAGCTCCAGTTTCAGCATTGTTCAGTGATGTCTATGATCACATACCCTCAAACCTTCATGAGCAAGAACAGGTACTGAGGCAAACAATGAAGAGGTATCCCCAAGATTACCCTTCTGATGTTCCTCTTTAG

Protein sequence

MIRTSLGLLSRDFPGGGGGARQNASVLLSSSNRSALLSPTRRFESPEAARLHEDENDAQELDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVSMKMYKDMITLQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLWRGFGLQEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMDRKDACAVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVVKGQAYGVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYRGVDEIEYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKKQKAEKAEKAPVSALFSDVYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL

Homology

[Close]

BLAST of CmaCh01G010170 vs. ExPASy Swiss-Prot
Match: Q9LPL5 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g21400 PE=2 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 4.5e-162
Identity = 279/395 (70.63%), Postives = 325/395 (82.28%), Query Frame = 0


Query: 54  DENDAQELDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVSMK 113
           DE DAQELDFPGG+V +TSEMKFI ESS +R+ C+R+L E+G  I  ++F  +S+ ++++
Sbjct: 73  DEVDAQELDFPGGKVGYTSEMKFIPESSSRRIPCYRVLDEDGRIIPDSDFIPVSEKLAVR 132

Query: 114 MYKDMITLQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVL 173
           MY+ M TLQ MD IF+EAQRQGRISFYLTS GEEA+ IASAAAL  DDVVL QYREPGVL
Sbjct: 133 MYEQMATLQVMDHIFYEAQRQGRISFYLTSVGEEAINIASAAALSPDDVVLPQYREPGVL 192

Query: 174 LWRGFGLQEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLK 233
           LWRGF L+EFA+Q FGN++DYGKGRQMPIHYGSN LN+ TISSPI TQLP A GV YSLK
Sbjct: 193 LWRGFTLEEFANQCFGNKADYGKGRQMPIHYGSNRLNYFTISSPIATQLPQAAGVGYSLK 252

Query: 234 MDRKDACAVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGV 293
           MD+K+AC VT+ GDGGTSEGDFHA LNFAAV+ APVVFICRNNGWAIST I EQFRSDG+
Sbjct: 253 MDKKNACTVTFIGDGGTSEGDFHAGLNFAAVMEAPVVFICRNNGWAISTHISEQFRSDGI 312

Query: 294 VVKGQAYGVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTK 353
           VVKGQAYG+RSIR+DGNDALAVYSA R AREMAV EQ PVLIE +TYRVGHHSTSDDSTK
Sbjct: 313 VVKGQAYGIRSIRVDGNDALAVYSAVRSAREMAVTEQRPVLIEMMTYRVGHHSTSDDSTK 372

Query: 354 YRGVDEIEYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKK------QKAEKAEKAP 413
           YR  DEI+YWK  R+P++RF +W+ +NGW SEEDES  RS  +K      Q AEK EK P
Sbjct: 373 YRAADEIQYWKMSRNPVNRFRKWVEDNGWWSEEDESKLRSNARKQLLQAIQAAEKWEKQP 432

Query: 414 VSALFSDVYDHIPSNLHEQEQVLRQTMKRYPQDYP 443
           ++ LF+DVYD  P NL EQE  L++ +K+ PQDYP
Sbjct: 433 LTELFNDVYDVKPKNLEEQELGLKELVKKQPQDYP 467

[Close]

BLAST of CmaCh01G010170 vs. ExPASy Swiss-Prot
Match: Q84JL2 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g09300 PE=1 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 1.0e-158
Identity = 269/402 (66.92%), Postives = 327/402 (81.34%), Query Frame = 0


Query: 52  HEDENDAQELDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVS 111
           HE  N+ Q +DFPGG+V FT E++FI+ES ++RV C+R+L +NG+ I  + F Q+S++V+
Sbjct: 71  HEYANNHQVMDFPGGKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVA 130

Query: 112 MKMYKDMITLQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPG 171
           +K+Y DM+TLQ MD IF+EAQRQGR+SFY T+ GEEA+ IASAAAL   DV+  QYREPG
Sbjct: 131 VKIYSDMVTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPG 190

Query: 172 VLLWRGFGLQEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYS 231
           VLLWRGF LQEFA+Q FGN+SDYGKGRQMP+HYGSN LN+ T+S+ I TQLP+AVG AYS
Sbjct: 191 VLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPNAVGAAYS 250

Query: 232 LKMDRKDACAVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSD 291
           LKMD+KDACAVTYFGDGGTSEGDFHAALN AAV+ APV+FICRNNGWAIST   +QFRSD
Sbjct: 251 LKMDKKDACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNGWAISTPTSDQFRSD 310

Query: 292 GVVVKGQAYGVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDS 351
           GVVVKG+AYG+RSIR+DGNDALA+YSA   AREMA+ EQ P+LIEALTYRVGHHSTSDDS
Sbjct: 311 GVVVKGRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEALTYRVGHHSTSDDS 370

Query: 352 TKYRGVDEIEYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKKQK------AEKAEK 411
           T+YR   EIE+W   R+P+ RF  W+ +NGW S++ ES  RS +KK+       AEK EK
Sbjct: 371 TRYRSAGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKKEMLEALRVAEKTEK 430

Query: 412 APVSALFSDVYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL 448
             +  +FSDVYD  PSNL EQE ++RQT+  +PQDYPSDVPL
Sbjct: 431 PNLQNMFSDVYDVPPSNLREQELLVRQTINSHPQDYPSDVPL 472

[Close]

BLAST of CmaCh01G010170 vs. ExPASy Swiss-Prot
Match: A5A6H9 (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Pan troglodytes OX=9598 GN=BCKDHA PE=2 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 4.6e-106
Identity = 185/388 (47.68%), Postives = 251/388 (64.69%), Query Frame = 0


Query: 63  FPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVSMKMYKDMITLQ 122
           FPG    F  +++FI  +    +  +R++   G+ I P+    L K+  +K+YK M  L 
Sbjct: 54  FPGASAEFIDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPKEKVLKLYKSMTLLN 113

Query: 123 TMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLWRGFGLQE 182
           TMD I +E+QRQGRISFY+T+ GEE   + SAAAL + D+V  QYRE GVL++R + L+ 
Sbjct: 114 TMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDNTDLVFGQYREAGVLMYRDYPLEL 173

Query: 183 FADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMDRKDACAV 242
           F  Q +GN SD GKGRQMP+HYG    +F+TISSP+ TQ+P AVG AY+ K    +   +
Sbjct: 174 FMAQCYGNISDLGKGRQMPVHYGCKERHFVTISSPLATQIPQAVGAAYAAKRANANRVVI 233

Query: 243 TYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVVKGQAYGV 302
            YFG+G  SEGD HA  NFAA L  P++F CRNNG+AIST   EQ+R DG+  +G  YG+
Sbjct: 234 CYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRGDGIAARGPGYGI 293

Query: 303 RSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYRGVDEIEY 362
            SIR+DGND  AVY+AT+ AR  AVAE  P LIEA+TYR+GHHSTSDDS+ YR VDE+ Y
Sbjct: 294 MSIRVDGNDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRSVDEVNY 353

Query: 363 WKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKK------QKAEKAEKAPVSALFSDVY 422
           W  +  P+ R   +L + GW  EE E   R   +K      ++AE+  K   + LFSDVY
Sbjct: 354 WDKQDHPVSRLRHYLLSQGWWDEEQEKAWRKQSRKKVMEAFEQAERKPKPNPNLLFSDVY 413

Query: 423 DHIPSNLHEQEQVLRQTMKRYPQDYPSD 445
             +P+ L +Q++ L + ++ Y + YP D
Sbjct: 414 QEMPAQLRKQQESLARHLQTYGEHYPLD 441

[Close]

BLAST of CmaCh01G010170 vs. ExPASy Swiss-Prot
Match: P11960 (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial (Fragment) OS=Rattus norvegicus OX=10116 GN=Bckdha PE=1 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 7.8e-106
Identity = 196/439 (44.65%), Postives = 263/439 (59.91%), Query Frame = 0


Query: 19  GARQNASVLLSSSNRSALLSPTRRFESPEAARLHEDENDAQEL-------DFPGGRVPFT 78
           G RQ A +LL       L            AR H      Q+         FPG    F 
Sbjct: 11  GLRQAALLLLGRPGARGL------------ARFHPSRQQQQQFPSLDDKPQFPGASAEFV 70

Query: 79  SEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVSMKMYKDMITLQTMDTIFFEA 138
            +++FI  +    +  +R++   G+ I P+    L ++  +K+Y+ M  L TMD I +E+
Sbjct: 71  DKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPQEEVLKLYRSMTLLNTMDRILYES 130

Query: 139 QRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLWRGFGLQEFADQVFGNR 198
           QRQGRISFY+T+ GEE   + SAAAL   D+V  QYRE GVL++R + L+ F  Q +GN 
Sbjct: 131 QRQGRISFYMTNYGEEGTHVGSAAALERTDLVFGQYREAGVLMYRDYPLELFMAQCYGNV 190

Query: 199 SDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMDRKDACAVTYFGDGGTS 258
           SD GKGRQMP+HYG    +F+TISSP+ TQ+P AVG AY+ K    +   + YFG+G  S
Sbjct: 191 SDPGKGRQMPVHYGCKERHFVTISSPLATQIPQAVGAAYAAKRANANQIVICYFGEGAAS 250

Query: 259 EGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVVKGQAYGVRSIRIDGND 318
           EGD HA  NFAA L  P++F CRNNG+AIST   EQ+R DG+  +G  YG+ SIR+DGND
Sbjct: 251 EGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRGDGIAARGPGYGIMSIRVDGND 310

Query: 319 ALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYRGVDEIEYWKTKRSPMD 378
             AVY+AT+ AR  AVAE  P LIEA+TYR+GHHSTSDDS+ YR VDE+ YW  +  P+ 
Sbjct: 311 VFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRSVDEVNYWDKQDHPIS 370

Query: 379 RFARWLRNNGWLSEEDESMHRSTVKK------QKAEKAEKAPVSALFSDVYDHIPSNLHE 438
           R  ++L N GW  EE E   R   +K      ++AE+  K   S LFSDVY  +P+ L  
Sbjct: 371 RLRQYLLNQGWWDEEQEKAWRKQSRKKVMEAFEQAERKLKPNPSLLFSDVYQEMPAQLRR 430

Query: 439 QEQVLRQTMKRYPQDYPSD 445
           Q++ L + ++ Y + YP D
Sbjct: 431 QQESLARHLQTYGEHYPLD 437

[Close]

BLAST of CmaCh01G010170 vs. ExPASy Swiss-Prot
Match: P12694 (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Homo sapiens OX=9606 GN=BCKDHA PE=1 SV=2)

HSP 1 Score: 385.2 bits (988), Expect = 1.0e-105
Identity = 184/388 (47.42%), Postives = 251/388 (64.69%), Query Frame = 0


Query: 63  FPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVSMKMYKDMITLQ 122
           FPG    F  +++FI  +    +  +R++   G+ I P+    L K+  +K+YK M  L 
Sbjct: 54  FPGASAEFIDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPKEKVLKLYKSMTLLN 113

Query: 123 TMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLWRGFGLQE 182
           TMD I +E+QRQGRISFY+T+ GEE   + SAAAL + D+V  QYRE GVL++R + L+ 
Sbjct: 114 TMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDNTDLVFGQYREAGVLMYRDYPLEL 173

Query: 183 FADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMDRKDACAV 242
           F  Q +GN SD GKGRQMP+HYG    +F+TISSP+ TQ+P AVG AY+ K    +   +
Sbjct: 174 FMAQCYGNISDLGKGRQMPVHYGCKERHFVTISSPLATQIPQAVGAAYAAKRANANRVVI 233

Query: 243 TYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVVKGQAYGV 302
            YFG+G  SEGD HA  NFAA L  P++F CRNNG+AIST   EQ+R DG+  +G  YG+
Sbjct: 234 CYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRGDGIAARGPGYGI 293

Query: 303 RSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYRGVDEIEY 362
            SIR+DGND  AVY+AT+ AR  AVAE  P LIEA+TYR+GHHSTSDDS+ YR VDE+ Y
Sbjct: 294 MSIRVDGNDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRSVDEVNY 353

Query: 363 WKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKK------QKAEKAEKAPVSALFSDVY 422
           W  +  P+ R   +L + GW  EE E   R   ++      ++AE+  K   + LFSDVY
Sbjct: 354 WDKQDHPISRLRHYLLSQGWWDEEQEKAWRKQSRRKVMEAFEQAERKPKPNPNLLFSDVY 413

Query: 423 DHIPSNLHEQEQVLRQTMKRYPQDYPSD 445
             +P+ L +Q++ L + ++ Y + YP D
Sbjct: 414 QEMPAQLRKQQESLARHLQTYGEHYPLD 441

[Close]

BLAST of CmaCh01G010170 vs. ExPASy TrEMBL
Match: A0A6J1IU18 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111480578 PE=4 SV=1)

HSP 1 Score: 891.0 bits (2301), Expect = 2.1e-255
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0


Query: 1   MIRTSLGLLSRDFPGGGGGARQNASVLLSSSNRSALLSPTRRFESPEAARLHEDENDAQE 60
           MIRTSLGLLSRDFPGGGGGARQNASVLLSSSNRSALLSPTRRFESPEAARLHEDENDAQE
Sbjct: 1   MIRTSLGLLSRDFPGGGGGARQNASVLLSSSNRSALLSPTRRFESPEAARLHEDENDAQE 60

Query: 61  LDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVSMKMYKDMIT 120
           LDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVSMKMYKDMIT
Sbjct: 61  LDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVSMKMYKDMIT 120

Query: 121 LQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLWRGFGL 180
           LQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLWRGFGL
Sbjct: 121 LQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLWRGFGL 180

Query: 181 QEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMDRKDAC 240
           QEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMDRKDAC
Sbjct: 181 QEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMDRKDAC 240

Query: 241 AVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVVKGQAY 300
           AVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVVKGQAY
Sbjct: 241 AVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVVKGQAY 300

Query: 301 GVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYRGVDEI 360
           GVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYRGVDEI
Sbjct: 301 GVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYRGVDEI 360

Query: 361 EYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKKQKAEKAEKAPVSALFSDVYDHIP 420
           EYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKKQKAEKAEKAPVSALFSDVYDHIP
Sbjct: 361 EYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKKQKAEKAEKAPVSALFSDVYDHIP 420

Query: 421 SNLHEQEQVLRQTMKRYPQDYPSDVPL 448
           SNLHEQEQVLRQTMKRYPQDYPSDVPL
Sbjct: 421 SNLHEQEQVLRQTMKRYPQDYPSDVPL 447

[Close]

BLAST of CmaCh01G010170 vs. ExPASy TrEMBL
Match: A0A6J1IWM6 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111480578 PE=4 SV=1)

HSP 1 Score: 884.4 bits (2284), Expect = 2.0e-253
Identity = 447/453 (98.68%), Postives = 447/453 (98.68%), Query Frame = 0


Query: 1   MIRTSLGLLSRDFPGGGGGARQNASVLLSSSNRSALLSPTRRFESPEAARLHEDENDAQE 60
           MIRTSLGLLSRDFPGGGGGARQNASVLLSSSNRSALLSPTRRFESPEAARLHEDENDAQE
Sbjct: 1   MIRTSLGLLSRDFPGGGGGARQNASVLLSSSNRSALLSPTRRFESPEAARLHEDENDAQE 60

Query: 61  LDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVSMKMYKDMIT 120
           LDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVSMKMYKDMIT
Sbjct: 61  LDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVSMKMYKDMIT 120

Query: 121 LQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLWRGFGL 180
           LQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLWRGFGL
Sbjct: 121 LQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLWRGFGL 180

Query: 181 QEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMDRKDAC 240
           QEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMDRKDAC
Sbjct: 181 QEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMDRKDAC 240

Query: 241 AVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVVKGQAY 300
           AVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVVKGQAY
Sbjct: 241 AVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVVKGQAY 300

Query: 301 GVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYRGVDEI 360
           GVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYRGVDEI
Sbjct: 301 GVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYRGVDEI 360

Query: 361 EYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKK------QKAEKAEKAPVSALFSD 420
           EYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKK      QKAEKAEKAPVSALFSD
Sbjct: 361 EYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKKQLLQAIQKAEKAEKAPVSALFSD 420

Query: 421 VYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL 448
           VYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL
Sbjct: 421 VYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL 453

[Close]

BLAST of CmaCh01G010170 vs. ExPASy TrEMBL
Match: A0A6J1J266 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111480578 PE=4 SV=1)

HSP 1 Score: 862.8 bits (2228), Expect = 6.1e-247
Identity = 439/453 (96.91%), Postives = 439/453 (96.91%), Query Frame = 0


Query: 1   MIRTSLGLLSRDFPGGGGGARQNASVLLSSSNRSALLSPTRRFESPEAARLHEDENDAQE 60
           MIRTSLGLLSRDFPGGGGGARQNASVLLSSSNRSALLSPTRRFESPEAARLHEDENDAQE
Sbjct: 1   MIRTSLGLLSRDFPGGGGGARQNASVLLSSSNRSALLSPTRRFESPEAARLHEDENDAQE 60

Query: 61  LDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVSMKMYKDMIT 120
           LDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQ        MYKDMIT
Sbjct: 61  LDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQ--------MYKDMIT 120

Query: 121 LQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLWRGFGL 180
           LQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLWRGFGL
Sbjct: 121 LQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLWRGFGL 180

Query: 181 QEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMDRKDAC 240
           QEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMDRKDAC
Sbjct: 181 QEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMDRKDAC 240

Query: 241 AVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVVKGQAY 300
           AVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVVKGQAY
Sbjct: 241 AVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVVKGQAY 300

Query: 301 GVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYRGVDEI 360
           GVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYRGVDEI
Sbjct: 301 GVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYRGVDEI 360

Query: 361 EYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKK------QKAEKAEKAPVSALFSD 420
           EYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKK      QKAEKAEKAPVSALFSD
Sbjct: 361 EYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKKQLLQAIQKAEKAEKAPVSALFSD 420

Query: 421 VYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL 448
           VYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL
Sbjct: 421 VYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL 445

[Close]

BLAST of CmaCh01G010170 vs. ExPASy TrEMBL
Match: A0A6J1EDX1 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111433389 PE=4 SV=1)

HSP 1 Score: 831.6 bits (2147), Expect = 1.5e-237
Identity = 422/458 (92.14%), Postives = 430/458 (93.89%), Query Frame = 0


Query: 1   MIRTSLGLLSRDFPG-----GGGGARQNASVLLSSSNRSALLSPTRRFESPEAARLHEDE 60
           MIRTSL LL+R+FP      G      NA VLLSSSNR ALLSPTRRFES EAARLHEDE
Sbjct: 1   MIRTSLALLTRNFPSPPPLLGAAAGPHNALVLLSSSNRYALLSPTRRFESTEAARLHEDE 60

Query: 61  NDAQELDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVSMKMY 120
           ND QE DFPGGRVPFTSEMKFIAES+E+RVSC+RILHENGETI P NFKQLSKDV +KMY
Sbjct: 61  NDGQEFDFPGGRVPFTSEMKFIAESTEERVSCYRILHENGETILPANFKQLSKDVMVKMY 120

Query: 121 KDMITLQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLW 180
           KDMITLQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLW
Sbjct: 121 KDMITLQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLW 180

Query: 181 RGFGLQEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMD 240
           RGFGLQEFADQVFGNRSDYGKGRQMPIHYGSNLLN+ TISSPIGTQLPHAVGVAYSLKMD
Sbjct: 181 RGFGLQEFADQVFGNRSDYGKGRQMPIHYGSNLLNYFTISSPIGTQLPHAVGVAYSLKMD 240

Query: 241 RKDACAVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVV 300
           RKDACAVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVV
Sbjct: 241 RKDACAVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVV 300

Query: 301 KGQAYGVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYR 360
           KGQAYGVRSIRIDGNDALAVYSATRRAREMA+AEQMPVLIEALTYRVGHHSTSDDSTKYR
Sbjct: 301 KGQAYGVRSIRIDGNDALAVYSATRRAREMAIAEQMPVLIEALTYRVGHHSTSDDSTKYR 360

Query: 361 GVDEIEYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKK------QKAEKAEKAPVS 420
           GVDEIEYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKK      QKAEKAEKAPVS
Sbjct: 361 GVDEIEYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKKQLLQAIQKAEKAEKAPVS 420

Query: 421 ALFSDVYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL 448
           ALFSDVYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL
Sbjct: 421 ALFSDVYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL 458

[Close]

BLAST of CmaCh01G010170 vs. ExPASy TrEMBL
Match: A0A6J1DJQ4 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021170 PE=4 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 9.3e-187
Identity = 328/420 (78.10%), Postives = 368/420 (87.62%), Query Frame = 0


Query: 40  TRRFESPEAARLHED------ENDAQELDFPGGRVPFTSEMKFIAESSEKRVSCFRILHE 99
           TRRFES EAA    D      ++  QELDFPGGRV FTSEM FIAES++KRV C+R+L +
Sbjct: 47  TRRFESTEAAAKXLDHLYCRNDDHPQELDFPGGRVSFTSEMSFIAESTQKRVPCYRVLDD 106

Query: 100 NGETIKPTNFKQLSKDVSMKMYKDMITLQTMDTIFFEAQRQGRISFYLTSAGEEAVTIAS 159
           NGE I   NF QL+KDV++KMYKDM+TLQTMDTIF+EAQRQGRISFYLTS+GEEA+TIAS
Sbjct: 107 NGERITSNNFTQLNKDVAVKMYKDMVTLQTMDTIFYEAQRQGRISFYLTSSGEEAITIAS 166

Query: 160 AAALHSDDVVLAQYREPGVLLWRGFGLQEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLT 219
           AAAL SDDVVLAQYREPGVLLWRGF LQEFADQ+FGN +DYGKGRQMPIHYGSN LN+ T
Sbjct: 167 AAALQSDDVVLAQYREPGVLLWRGFTLQEFADQLFGNIADYGKGRQMPIHYGSNKLNYFT 226

Query: 220 ISSPIGTQLPHAVGVAYSLKMDRKDACAVTYFGDGGTSEGDFHAALNFAAVLGAPVVFIC 279
           ISSP+ TQLP AVGV YSLKMD+KDACAVTYFGDG TSEGDFHAALNFAAVL APV+FIC
Sbjct: 227 ISSPLATQLPQAVGVGYSLKMDKKDACAVTYFGDGTTSEGDFHAALNFAAVLRAPVIFIC 286

Query: 280 RNNGWAISTSIQEQFRSDGVVVKGQAYGVRSIRIDGNDALAVYSATRRAREMAVAEQMPV 339
           RNNGWAISTS++EQFRSDGVVVKGQAYG+RSIRIDGNDALAVY+A RRAR MAVAEQ PV
Sbjct: 287 RNNGWAISTSVEEQFRSDGVVVKGQAYGIRSIRIDGNDALAVYTAIRRARAMAVAEQAPV 346

Query: 340 LIEALTYRVGHHSTSDDSTKYRGVDEIEYWKTKRSPMDRFARWLRNNGWLSEEDESMHRS 399
           L+EALTYRVGHHSTSDDS+KYR +DEI+YWK +R+P++RF+ WL  N WL++EDES HR+
Sbjct: 347 LVEALTYRVGHHSTSDDSSKYRAMDEIQYWKMERNPVNRFSNWLTTNRWLTQEDESNHRT 406

Query: 400 TVKK------QKAEKAEKAPVSALFSDVYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL 448
           TVK+      Q AE+ EK PVS LFSDVYD++PSNLHEQEQVLRQTMKRYPQDYPSDVPL
Sbjct: 407 TVKQQLLQAIQNAERREKPPVSELFSDVYDNVPSNLHEQEQVLRQTMKRYPQDYPSDVPL 466

[Close]

BLAST of CmaCh01G010170 vs. NCBI nr
Match: XP_022981467.1 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 891.0 bits (2301), Expect = 4.4e-255
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0


Query: 1   MIRTSLGLLSRDFPGGGGGARQNASVLLSSSNRSALLSPTRRFESPEAARLHEDENDAQE 60
           MIRTSLGLLSRDFPGGGGGARQNASVLLSSSNRSALLSPTRRFESPEAARLHEDENDAQE
Sbjct: 1   MIRTSLGLLSRDFPGGGGGARQNASVLLSSSNRSALLSPTRRFESPEAARLHEDENDAQE 60

Query: 61  LDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVSMKMYKDMIT 120
           LDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVSMKMYKDMIT
Sbjct: 61  LDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVSMKMYKDMIT 120

Query: 121 LQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLWRGFGL 180
           LQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLWRGFGL
Sbjct: 121 LQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLWRGFGL 180

Query: 181 QEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMDRKDAC 240
           QEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMDRKDAC
Sbjct: 181 QEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMDRKDAC 240

Query: 241 AVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVVKGQAY 300
           AVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVVKGQAY
Sbjct: 241 AVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVVKGQAY 300

Query: 301 GVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYRGVDEI 360
           GVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYRGVDEI
Sbjct: 301 GVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYRGVDEI 360

Query: 361 EYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKKQKAEKAEKAPVSALFSDVYDHIP 420
           EYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKKQKAEKAEKAPVSALFSDVYDHIP
Sbjct: 361 EYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKKQKAEKAEKAPVSALFSDVYDHIP 420

Query: 421 SNLHEQEQVLRQTMKRYPQDYPSDVPL 448
           SNLHEQEQVLRQTMKRYPQDYPSDVPL
Sbjct: 421 SNLHEQEQVLRQTMKRYPQDYPSDVPL 447

[Close]

BLAST of CmaCh01G010170 vs. NCBI nr
Match: XP_022981466.1 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 884.4 bits (2284), Expect = 4.1e-253
Identity = 447/453 (98.68%), Postives = 447/453 (98.68%), Query Frame = 0


Query: 1   MIRTSLGLLSRDFPGGGGGARQNASVLLSSSNRSALLSPTRRFESPEAARLHEDENDAQE 60
           MIRTSLGLLSRDFPGGGGGARQNASVLLSSSNRSALLSPTRRFESPEAARLHEDENDAQE
Sbjct: 1   MIRTSLGLLSRDFPGGGGGARQNASVLLSSSNRSALLSPTRRFESPEAARLHEDENDAQE 60

Query: 61  LDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVSMKMYKDMIT 120
           LDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVSMKMYKDMIT
Sbjct: 61  LDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVSMKMYKDMIT 120

Query: 121 LQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLWRGFGL 180
           LQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLWRGFGL
Sbjct: 121 LQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLWRGFGL 180

Query: 181 QEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMDRKDAC 240
           QEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMDRKDAC
Sbjct: 181 QEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMDRKDAC 240

Query: 241 AVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVVKGQAY 300
           AVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVVKGQAY
Sbjct: 241 AVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVVKGQAY 300

Query: 301 GVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYRGVDEI 360
           GVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYRGVDEI
Sbjct: 301 GVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYRGVDEI 360

Query: 361 EYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKK------QKAEKAEKAPVSALFSD 420
           EYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKK      QKAEKAEKAPVSALFSD
Sbjct: 361 EYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKKQLLQAIQKAEKAEKAPVSALFSD 420

Query: 421 VYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL 448
           VYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL
Sbjct: 421 VYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL 453

[Close]

BLAST of CmaCh01G010170 vs. NCBI nr
Match: XP_022981468.1 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 862.8 bits (2228), Expect = 1.3e-246
Identity = 439/453 (96.91%), Postives = 439/453 (96.91%), Query Frame = 0


Query: 1   MIRTSLGLLSRDFPGGGGGARQNASVLLSSSNRSALLSPTRRFESPEAARLHEDENDAQE 60
           MIRTSLGLLSRDFPGGGGGARQNASVLLSSSNRSALLSPTRRFESPEAARLHEDENDAQE
Sbjct: 1   MIRTSLGLLSRDFPGGGGGARQNASVLLSSSNRSALLSPTRRFESPEAARLHEDENDAQE 60

Query: 61  LDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVSMKMYKDMIT 120
           LDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQ        MYKDMIT
Sbjct: 61  LDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQ--------MYKDMIT 120

Query: 121 LQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLWRGFGL 180
           LQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLWRGFGL
Sbjct: 121 LQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLWRGFGL 180

Query: 181 QEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMDRKDAC 240
           QEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMDRKDAC
Sbjct: 181 QEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMDRKDAC 240

Query: 241 AVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVVKGQAY 300
           AVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVVKGQAY
Sbjct: 241 AVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVVKGQAY 300

Query: 301 GVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYRGVDEI 360
           GVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYRGVDEI
Sbjct: 301 GVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYRGVDEI 360

Query: 361 EYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKK------QKAEKAEKAPVSALFSD 420
           EYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKK      QKAEKAEKAPVSALFSD
Sbjct: 361 EYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKKQLLQAIQKAEKAEKAPVSALFSD 420

Query: 421 VYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL 448
           VYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL
Sbjct: 421 VYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL 445

[Close]

BLAST of CmaCh01G010170 vs. NCBI nr
Match: KAG6607691.1 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 841.3 bits (2172), Expect = 4.0e-240
Identity = 425/458 (92.79%), Postives = 435/458 (94.98%), Query Frame = 0


Query: 1   MIRTSLGLLSRDFPG-----GGGGARQNASVLLSSSNRSALLSPTRRFESPEAARLHEDE 60
           MIRTSLGLL+R+FP      G   AR NA VLLSSSNR ALLSPTRRFES EAARLHEDE
Sbjct: 1   MIRTSLGLLTRNFPSPPPLLGAAAARHNALVLLSSSNRYALLSPTRRFESTEAARLHEDE 60

Query: 61  NDAQELDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVSMKMY 120
           ND QELDFPGGRVPFTSEMKFIAES+EKRVSC+RILHENGETI PTNFKQLSKDV +KMY
Sbjct: 61  NDGQELDFPGGRVPFTSEMKFIAESTEKRVSCYRILHENGETILPTNFKQLSKDVMVKMY 120

Query: 121 KDMITLQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLW 180
           KDMITLQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLW
Sbjct: 121 KDMITLQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLW 180

Query: 181 RGFGLQEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMD 240
           RGFGLQEFADQVFGNRSDYGKGRQMPIHYGSNLLN+ T+SSPIGTQLPHAVGVAYSLKMD
Sbjct: 181 RGFGLQEFADQVFGNRSDYGKGRQMPIHYGSNLLNYFTVSSPIGTQLPHAVGVAYSLKMD 240

Query: 241 RKDACAVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVV 300
           RKDACAVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVV+
Sbjct: 241 RKDACAVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVI 300

Query: 301 KGQAYGVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYR 360
           KGQAYGVRSIRIDGNDALAVYSATRRAREMA+AEQMPVLIEALTYRVGHHSTSDDSTKYR
Sbjct: 301 KGQAYGVRSIRIDGNDALAVYSATRRAREMAIAEQMPVLIEALTYRVGHHSTSDDSTKYR 360

Query: 361 GVDEIEYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKK------QKAEKAEKAPVS 420
           GVDEIEYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKK      QKAEKAEKAPVS
Sbjct: 361 GVDEIEYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKKQLLQAIQKAEKAEKAPVS 420

Query: 421 ALFSDVYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL 448
           ALF+DVYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL
Sbjct: 421 ALFNDVYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL 458

[Close]

BLAST of CmaCh01G010170 vs. NCBI nr
Match: XP_022926207.1 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 831.6 bits (2147), Expect = 3.1e-237
Identity = 422/458 (92.14%), Postives = 430/458 (93.89%), Query Frame = 0


Query: 1   MIRTSLGLLSRDFPG-----GGGGARQNASVLLSSSNRSALLSPTRRFESPEAARLHEDE 60
           MIRTSL LL+R+FP      G      NA VLLSSSNR ALLSPTRRFES EAARLHEDE
Sbjct: 1   MIRTSLALLTRNFPSPPPLLGAAAGPHNALVLLSSSNRYALLSPTRRFESTEAARLHEDE 60

Query: 61  NDAQELDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVSMKMY 120
           ND QE DFPGGRVPFTSEMKFIAES+E+RVSC+RILHENGETI P NFKQLSKDV +KMY
Sbjct: 61  NDGQEFDFPGGRVPFTSEMKFIAESTEERVSCYRILHENGETILPANFKQLSKDVMVKMY 120

Query: 121 KDMITLQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLW 180
           KDMITLQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLW
Sbjct: 121 KDMITLQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLW 180

Query: 181 RGFGLQEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMD 240
           RGFGLQEFADQVFGNRSDYGKGRQMPIHYGSNLLN+ TISSPIGTQLPHAVGVAYSLKMD
Sbjct: 181 RGFGLQEFADQVFGNRSDYGKGRQMPIHYGSNLLNYFTISSPIGTQLPHAVGVAYSLKMD 240

Query: 241 RKDACAVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVV 300
           RKDACAVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVV
Sbjct: 241 RKDACAVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVV 300

Query: 301 KGQAYGVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYR 360
           KGQAYGVRSIRIDGNDALAVYSATRRAREMA+AEQMPVLIEALTYRVGHHSTSDDSTKYR
Sbjct: 301 KGQAYGVRSIRIDGNDALAVYSATRRAREMAIAEQMPVLIEALTYRVGHHSTSDDSTKYR 360

Query: 361 GVDEIEYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKK------QKAEKAEKAPVS 420
           GVDEIEYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKK      QKAEKAEKAPVS
Sbjct: 361 GVDEIEYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKKQLLQAIQKAEKAEKAPVS 420

Query: 421 ALFSDVYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL 448
           ALFSDVYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL
Sbjct: 421 ALFSDVYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL 458

[Close]

BLAST of CmaCh01G010170 vs. TAIR 10
Match: AT1G21400.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 572.4 bits (1474), Expect = 3.2e-163
Identity = 279/395 (70.63%), Postives = 325/395 (82.28%), Query Frame = 0


Query: 54  DENDAQELDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVSMK 113
           DE DAQELDFPGG+V +TSEMKFI ESS +R+ C+R+L E+G  I  ++F  +S+ ++++
Sbjct: 73  DEVDAQELDFPGGKVGYTSEMKFIPESSSRRIPCYRVLDEDGRIIPDSDFIPVSEKLAVR 132

Query: 114 MYKDMITLQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVL 173
           MY+ M TLQ MD IF+EAQRQGRISFYLTS GEEA+ IASAAAL  DDVVL QYREPGVL
Sbjct: 133 MYEQMATLQVMDHIFYEAQRQGRISFYLTSVGEEAINIASAAALSPDDVVLPQYREPGVL 192

Query: 174 LWRGFGLQEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLK 233
           LWRGF L+EFA+Q FGN++DYGKGRQMPIHYGSN LN+ TISSPI TQLP A GV YSLK
Sbjct: 193 LWRGFTLEEFANQCFGNKADYGKGRQMPIHYGSNRLNYFTISSPIATQLPQAAGVGYSLK 252

Query: 234 MDRKDACAVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGV 293
           MD+K+AC VT+ GDGGTSEGDFHA LNFAAV+ APVVFICRNNGWAIST I EQFRSDG+
Sbjct: 253 MDKKNACTVTFIGDGGTSEGDFHAGLNFAAVMEAPVVFICRNNGWAISTHISEQFRSDGI 312

Query: 294 VVKGQAYGVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTK 353
           VVKGQAYG+RSIR+DGNDALAVYSA R AREMAV EQ PVLIE +TYRVGHHSTSDDSTK
Sbjct: 313 VVKGQAYGIRSIRVDGNDALAVYSAVRSAREMAVTEQRPVLIEMMTYRVGHHSTSDDSTK 372

Query: 354 YRGVDEIEYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKK------QKAEKAEKAP 413
           YR  DEI+YWK  R+P++RF +W+ +NGW SEEDES  RS  +K      Q AEK EK P
Sbjct: 373 YRAADEIQYWKMSRNPVNRFRKWVEDNGWWSEEDESKLRSNARKQLLQAIQAAEKWEKQP 432

Query: 414 VSALFSDVYDHIPSNLHEQEQVLRQTMKRYPQDYP 443
           ++ LF+DVYD  P NL EQE  L++ +K+ PQDYP
Sbjct: 433 LTELFNDVYDVKPKNLEEQELGLKELVKKQPQDYP 467

[Close]

BLAST of CmaCh01G010170 vs. TAIR 10
Match: AT5G09300.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 561.2 bits (1445), Expect = 7.4e-160
Identity = 269/402 (66.92%), Postives = 327/402 (81.34%), Query Frame = 0


Query: 52  HEDENDAQELDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVS 111
           HE  N+ Q +DFPGG+V FT E++FI+ES ++RV C+R+L +NG+ I  + F Q+S++V+
Sbjct: 71  HEYANNHQVMDFPGGKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVA 130

Query: 112 MKMYKDMITLQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPG 171
           +K+Y DM+TLQ MD IF+EAQRQGR+SFY T+ GEEA+ IASAAAL   DV+  QYREPG
Sbjct: 131 VKIYSDMVTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPG 190

Query: 172 VLLWRGFGLQEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYS 231
           VLLWRGF LQEFA+Q FGN+SDYGKGRQMP+HYGSN LN+ T+S+ I TQLP+AVG AYS
Sbjct: 191 VLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPNAVGAAYS 250

Query: 232 LKMDRKDACAVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSD 291
           LKMD+KDACAVTYFGDGGTSEGDFHAALN AAV+ APV+FICRNNGWAIST   +QFRSD
Sbjct: 251 LKMDKKDACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNGWAISTPTSDQFRSD 310

Query: 292 GVVVKGQAYGVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDS 351
           GVVVKG+AYG+RSIR+DGNDALA+YSA   AREMA+ EQ P+LIEALTYRVGHHSTSDDS
Sbjct: 311 GVVVKGRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEALTYRVGHHSTSDDS 370

Query: 352 TKYRGVDEIEYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKKQK------AEKAEK 411
           T+YR   EIE+W   R+P+ RF  W+ +NGW S++ ES  RS +KK+       AEK EK
Sbjct: 371 TRYRSAGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKKEMLEALRVAEKTEK 430

Query: 412 APVSALFSDVYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL 448
             +  +FSDVYD  PSNL EQE ++RQT+  +PQDYPSDVPL
Sbjct: 431 PNLQNMFSDVYDVPPSNLREQELLVRQTINSHPQDYPSDVPL 472

[Close]

BLAST of CmaCh01G010170 vs. TAIR 10
Match: AT5G09300.2 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 557.0 bits (1434), Expect = 1.4e-158
Identity = 266/397 (67.00%), Postives = 324/397 (81.61%), Query Frame = 0


Query: 57  DAQELDFPGGRVPFTSEMKFIAESSEKRVSCFRILHENGETIKPTNFKQLSKDVSMKMYK 116
           + Q +DFPGG+V FT E++FI+ES ++RV C+R+L +NG+ I  + F Q+S++V++K+Y 
Sbjct: 5   NVQVMDFPGGKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYS 64

Query: 117 DMITLQTMDTIFFEAQRQGRISFYLTSAGEEAVTIASAAALHSDDVVLAQYREPGVLLWR 176
           DM+TLQ MD IF+EAQRQGR+SFY T+ GEEA+ IASAAAL   DV+  QYREPGVLLWR
Sbjct: 65  DMVTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWR 124

Query: 177 GFGLQEFADQVFGNRSDYGKGRQMPIHYGSNLLNFLTISSPIGTQLPHAVGVAYSLKMDR 236
           GF LQEFA+Q FGN+SDYGKGRQMP+HYGSN LN+ T+S+ I TQLP+AVG AYSLKMD+
Sbjct: 125 GFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPNAVGAAYSLKMDK 184

Query: 237 KDACAVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQEQFRSDGVVVK 296
           KDACAVTYFGDGGTSEGDFHAALN AAV+ APV+FICRNNGWAIST   +QFRSDGVVVK
Sbjct: 185 KDACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNGWAISTPTSDQFRSDGVVVK 244

Query: 297 GQAYGVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHHSTSDDSTKYRG 356
           G+AYG+RSIR+DGNDALA+YSA   AREMA+ EQ P+LIEALTYRVGHHSTSDDST+YR 
Sbjct: 245 GRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEALTYRVGHHSTSDDSTRYRS 304

Query: 357 VDEIEYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKKQK------AEKAEKAPVSA 416
             EIE+W   R+P+ RF  W+ +NGW S++ ES  RS +KK+       AEK EK  +  
Sbjct: 305 AGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKKEMLEALRVAEKTEKPNLQN 364

Query: 417 LFSDVYDHIPSNLHEQEQVLRQTMKRYPQDYPSDVPL 448
           +FSDVYD  PSNL EQE ++RQT+  +PQDYPSDVPL
Sbjct: 365 MFSDVYDVPPSNLREQELLVRQTINSHPQDYPSDVPL 401

[Close]

BLAST of CmaCh01G010170 vs. TAIR 10
Match: AT5G34780.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 291.2 bits (744), Expect = 1.4e-78
Identity = 145/222 (65.32%), Postives = 173/222 (77.93%), Query Frame = 0


Query: 226 VGVAYSLKMDRKDACAVTYFGDGGTSEGDFHAALNFAAVLGAPVVFICRNNGWAISTSIQ 285
           +G++ +     K+ACAVT+ GDGGTSEGDFHA LNFAAV+ APVVFICRNNGWAIST I 
Sbjct: 15  IGLSKAKDCWEKNACAVTFIGDGGTSEGDFHAGLNFAAVMEAPVVFICRNNGWAISTHIS 74

Query: 286 EQFRSDGVVVKGQAYGVRSIRIDGNDALAVYSATRRAREMAVAEQMPVLIEALTYRVGHH 345
           EQFRSDG+VVKGQAYG+RSIR+DGNDALAVYSA   AREMAV EQ PVLIE + YRVGHH
Sbjct: 75  EQFRSDGIVVKGQAYGIRSIRVDGNDALAVYSAVCSAREMAVTEQRPVLIEMMIYRVGHH 134

Query: 346 STSDDSTKYRGVDEIEYWKTKRSPMDRFARWLRNNGWLSEEDESMHRSTVKK------QK 405
           STSDDSTKYR  DEI+YWK  R+ ++RF + + +NGW SEEDES  RS  +K      Q 
Sbjct: 135 STSDDSTKYRAADEIQYWKMSRNSVNRFRKSVEDNGWWSEEDESKLRSNARKQLLQAIQA 194

Query: 406 AEKAEKAPVSALFSDVYDHIPSNLHEQEQVLRQTMKRYPQDY 442
           AEK EK P++ LF+DVYD  P NL E+E  L++ +++ PQDY
Sbjct: 195 AEKWEKQPLTELFNDVYDVKPKNLEEEELGLKELIEKQPQDY 236

[Close]

BLAST of CmaCh01G010170 vs. TAIR 10
Match: AT1G59900.1 (pyruvate dehydrogenase complex E1 alpha subunit )

HSP 1 Score: 136.7 bits (343), Expect = 4.5e-32
Identity = 80/278 (28.78%), Postives = 146/278 (52.52%), Query Frame = 0


Query: 145 GEEAVTIASAAALHSDDVVLAQYREPGVLLWRGFGLQEFADQVFGNRSDYGKGRQMPIHY 204
           G+EAV I   AA+   D ++  YR+  + L RG  L E   ++ G ++   KG+   +H+
Sbjct: 94  GQEAVAIGMEAAITKKDAIITAYRDHCIFLGRGGSLHEVFSELMGRQAGCSKGKGGSMHF 153

Query: 205 GSNLLNFLTISSPIGTQLPHAVGVAYSLKMDRKDACAVTYFGDGGTSEGDFHAALNFAAV 264
                +F      +G Q+P   G+A++ K ++++A     +GDG  ++G    ALN +A+
Sbjct: 154 YKKESSFYGGHGIVGAQVPLGCGIAFAQKYNKEEAVTFALYGDGAANQGQLFEALNISAL 213

Query: 265 LGAPVVFICRNNGWAISTSIQEQFRSDGVVVKGQAYGVRSIRIDGNDALAVYSATRRARE 324
              P + +C NN + + T+     +S     +G    V  +++DG DA AV  A + A++
Sbjct: 214 WDLPAILVCENNHYGMGTAEWRAAKSPSYYKRGDY--VPGLKVDGMDAFAVKQACKFAKQ 273

Query: 325 MAVAEQMPVLIEALTYRVGHHSTSDDSTKYRGVDEIEYWKTKRSPMDRFARWLRNNGWLS 384
            A+ E+ P+++E  TYR   HS SD  + YR  DEI   + +R P++R  + + ++   +
Sbjct: 274 HAL-EKGPIILEMDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERIKKLVLSHDLAT 333

Query: 385 EEDESMHRSTVKKQKAE---KAEKAPV---SALFSDVY 417
           E++       ++K+  +   KA+  P+   S LF++VY
Sbjct: 334 EKELKDMEKEIRKEVDDAIAKAKDCPMPEPSELFTNVY 368

The following BLAST results are available for this feature:

BLAST of CmaCh01G010170 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LPL5	4.5e-162	70.63	2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial OS=Arabidopsis tha...	[more]
Q84JL2	1.0e-158	66.92	2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Arabidopsis tha...	[more]
A5A6H9	4.6e-106	47.68	2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Pan troglodytes O...	[more]
P11960	7.8e-106	44.65	2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial (Fragment) OS=Rattus...	[more]
P12694	1.0e-105	47.42	2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Homo sapiens OX=9...	[more]

BLAST of CmaCh01G010170 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IU18	2.1e-255	100.00	2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like isoform X2 OS...	[more]
A0A6J1IWM6	2.0e-253	98.68	2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like isoform X1 OS...	[more]
A0A6J1J266	6.1e-247	96.91	2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like isoform X3 OS...	[more]
A0A6J1EDX1	1.5e-237	92.14	2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like OS=Cucurbita ...	[more]
A0A6J1DJQ4	9.3e-187	78.10	2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like isoform X1 OS...	[more]

BLAST of CmaCh01G010170 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022981467.1	4.4e-255	100.00	2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like isoform X2 [C...	[more]
XP_022981466.1	4.1e-253	98.68	2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like isoform X1 [C...	[more]
XP_022981468.1	1.3e-246	96.91	2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like isoform X3 [C...	[more]
KAG6607691.1	4.0e-240	92.79	2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial, partial [Cucurbit...	[more]
XP_022926207.1	3.1e-237	92.14	2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like [Cucurbita mo...	[more]

BLAST of CmaCh01G010170 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G21400.1	3.2e-163	70.63	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]
AT5G09300.1	7.4e-160	66.92	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]
AT5G09300.2	1.4e-158	67.00	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]
AT5G34780.1	1.4e-78	65.32	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]
AT1G59900.1	4.5e-32	28.78	pyruvate dehydrogenase complex E1 alpha subunit	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001017	Dehydrogenase, E1 component	PFAM	PF00676	E1_dh	coord: 116..407 e-value: 6.7E-89 score: 297.8
None	No IPR available	GENE3D	3.40.50.970		coord: 56..445 e-value: 4.1E-138 score: 462.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 37..66
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 45..61
None	No IPR available	PANTHER	PTHR43380	2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL	coord: 39..447
None	No IPR available	PANTHER	PTHR43380:SF11	2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA 2, MITOCHONDRIAL	coord: 39..447
None	No IPR available	CDD	cd02000	TPP_E1_PDC_ADC_BCADC	coord: 114..388 e-value: 1.13304E-137 score: 394.94
IPR029061	Thiamin diphosphate-binding fold	SUPERFAMILY	52518	Thiamin diphosphate-binding fold (THDP-binding)	coord: 88..442

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh01G010170.1	CmaCh01G010170.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009083	branched-chain amino acid catabolic process
cellular_component	GO:0005947	mitochondrial alpha-ketoglutarate dehydrogenase complex
molecular_function	GO:0016624	oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor