CmaCh01G009040 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh01G009040
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionDNA-directed RNA polymerase subunit beta''
LocationCma_Chr01: 5370193 .. 5370511 (+)
RNA-Seq ExpressionCmaCh01G009040
SyntenyCmaCh01G009040
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCCCACGATGCATTGGCATACGAAATCAAGATATTGGTATTGGACTTATCAATCGATTCATAACCTTTCAAACACAACCGATATCTATTCGGACTCCCTTTACTTGTAGGAGTACATCTTGGATCTGCCGATTATGTTATGGACGAAGTCCTACTCACGGGGACCTGGTCGAATTGGGAGAAGCCGTAGGTATTATTGCGGGTCAATCTATTGGAGAACCGGGTACTCAACTAACATTAAGAACTTTTCATACCGGCGGAGTATTCACAGGGGGGACTGCAGAACATATACGAGCTCCTTCTAATGGAAAAATAA

mRNA sequence

ATGGGCCCACGATGCATTGGCATACGAAATCAAGATATTGGTATTGGACTTATCAATCGATTCATAACCTTTCAAACACAACCGATATCTATTCGGACTCCCTTTACTTGTAGGAGTACATCTTGGATCTGCCGATTATGTTATGGACGAAGTCCTACTCACGGGGACCTGGTCGAATTGGGAGAAGCCGTAGGTATTATTGCGGGTCAATCTATTGGAGAACCGGAACTTTTCATACCGGCGGAGTATTCACAGGGGGGACTGCAGAACATATACGAGCTCCTTCTAATGGAAAAATAA

Coding sequence (CDS)

ATGGGCCCACGATGCATTGGCATACGAAATCAAGATATTGGTATTGGACTTATCAATCGATTCATAACCTTTCAAACACAACCGATATCTATTCGGACTCCCTTTACTTGTAGGAGTACATCTTGGATCTGCCGATTATGTTATGGACGAAGTCCTACTCACGGGGACCTGGTCGAATTGGGAGAAGCCGTAGGTATTATTGCGGGTCAATCTATTGGAGAACCGGAACTTTTCATACCGGCGGAGTATTCACAGGGGGGACTGCAGAACATATACGAGCTCCTTCTAATGGAAAAATAA

Protein sequence

MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVELGEAVGIIAGQSIGEPELFIPAEYSQGGLQNIYELLLMEK
Homology
BLAST of CmaCh01G009040 vs. ExPASy Swiss-Prot
Match: Q4VZP3 (DNA-directed RNA polymerase subunit beta'' OS=Cucumis sativus OX=3659 GN=rpoC2 PE=3 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 2.4e-39
Identity = 74/75 (98.67%), Postives = 75/75 (100.00%), Query Frame = 0

Query: 1   MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 60
           MGPRCIG+RNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL
Sbjct: 259 MGPRCIGVRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 318

Query: 61  GEAVGIIAGQSIGEP 76
           GEAVGIIAGQSIGEP
Sbjct: 319 GEAVGIIAGQSIGEP 333

BLAST of CmaCh01G009040 vs. ExPASy Swiss-Prot
Match: Q49L08 (DNA-directed RNA polymerase subunit beta'' OS=Eucalyptus globulus subsp. globulus OX=71271 GN=rpoC2 PE=3 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 3.4e-38
Identity = 72/75 (96.00%), Postives = 74/75 (98.67%), Query Frame = 0

Query: 1   MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 60
           MGPRCI IRNQDIGIGL+NRFITF+TQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL
Sbjct: 259 MGPRCIAIRNQDIGIGLVNRFITFRTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 318

Query: 61  GEAVGIIAGQSIGEP 76
           GEAVGIIAGQSIGEP
Sbjct: 319 GEAVGIIAGQSIGEP 333

BLAST of CmaCh01G009040 vs. ExPASy Swiss-Prot
Match: B1A925 (DNA-directed RNA polymerase subunit beta'' OS=Carica papaya OX=3649 GN=rpoC2 PE=3 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 2.9e-37
Identity = 71/75 (94.67%), Postives = 73/75 (97.33%), Query Frame = 0

Query: 1   MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 60
           MGPRCI IRNQDIGIGL+NRFITF+TQ ISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL
Sbjct: 255 MGPRCIAIRNQDIGIGLVNRFITFRTQSISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 314

Query: 61  GEAVGIIAGQSIGEP 76
           GEAVGIIAGQSIGEP
Sbjct: 315 GEAVGIIAGQSIGEP 329

BLAST of CmaCh01G009040 vs. ExPASy Swiss-Prot
Match: Q8S8Y1 (DNA-directed RNA polymerase subunit beta'' OS=Atropa belladonna OX=33113 GN=rpoC2 PE=3 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 5.0e-37
Identity = 70/75 (93.33%), Postives = 72/75 (96.00%), Query Frame = 0

Query: 1   MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 60
           MGPRCI  RNQDIGIGL+NRFITF+ QPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL
Sbjct: 259 MGPRCIATRNQDIGIGLVNRFITFRAQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 318

Query: 61  GEAVGIIAGQSIGEP 76
           GEAVGIIAGQSIGEP
Sbjct: 319 GEAVGIIAGQSIGEP 333

BLAST of CmaCh01G009040 vs. ExPASy Swiss-Prot
Match: Q3C1G9 (DNA-directed RNA polymerase subunit beta'' OS=Nicotiana sylvestris OX=4096 GN=rpoC2 PE=3 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 5.0e-37
Identity = 70/75 (93.33%), Postives = 72/75 (96.00%), Query Frame = 0

Query: 1   MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 60
           MGPRCI  RNQDIGIGL+NRFITF+ QPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL
Sbjct: 259 MGPRCIATRNQDIGIGLVNRFITFRAQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 318

Query: 61  GEAVGIIAGQSIGEP 76
           GEAVGIIAGQSIGEP
Sbjct: 319 GEAVGIIAGQSIGEP 333

BLAST of CmaCh01G009040 vs. ExPASy TrEMBL
Match: A0A6M3RTH8 (DNA-directed RNA polymerase subunit beta'' OS=Trichosanthes kirilowii OX=3677 GN=rpoC2 PE=3 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 6.7e-37
Identity = 75/75 (100.00%), Postives = 75/75 (100.00%), Query Frame = 0

Query: 1   MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 60
           MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL
Sbjct: 259 MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 318

Query: 61  GEAVGIIAGQSIGEP 76
           GEAVGIIAGQSIGEP
Sbjct: 319 GEAVGIIAGQSIGEP 333

BLAST of CmaCh01G009040 vs. ExPASy TrEMBL
Match: A0A6M3S448 (DNA-directed RNA polymerase subunit beta'' OS=Trichosanthes kirilowii var. japonica OX=61011 GN=rpoC2 PE=3 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 6.7e-37
Identity = 75/75 (100.00%), Postives = 75/75 (100.00%), Query Frame = 0

Query: 1   MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 60
           MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL
Sbjct: 259 MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 318

Query: 61  GEAVGIIAGQSIGEP 76
           GEAVGIIAGQSIGEP
Sbjct: 319 GEAVGIIAGQSIGEP 333

BLAST of CmaCh01G009040 vs. ExPASy TrEMBL
Match: A0A346QNZ0 (DNA-directed RNA polymerase subunit beta'' OS=Hodgsonia macrocarpa OX=2184935 GN=rpoC2 PE=3 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 6.7e-37
Identity = 75/75 (100.00%), Postives = 75/75 (100.00%), Query Frame = 0

Query: 1   MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 60
           MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL
Sbjct: 301 MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 360

Query: 61  GEAVGIIAGQSIGEP 76
           GEAVGIIAGQSIGEP
Sbjct: 361 GEAVGIIAGQSIGEP 375

BLAST of CmaCh01G009040 vs. ExPASy TrEMBL
Match: A0A6H0EPT4 (DNA-directed RNA polymerase subunit beta'' OS=Luffa aegyptiaca OX=3670 GN=rpoC2 PE=3 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 6.7e-37
Identity = 75/75 (100.00%), Postives = 75/75 (100.00%), Query Frame = 0

Query: 1   MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 60
           MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL
Sbjct: 255 MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 314

Query: 61  GEAVGIIAGQSIGEP 76
           GEAVGIIAGQSIGEP
Sbjct: 315 GEAVGIIAGQSIGEP 329

BLAST of CmaCh01G009040 vs. ExPASy TrEMBL
Match: A0A514YKS0 (DNA-directed RNA polymerase subunit beta'' OS=Siraitia grosvenorii OX=190515 GN=rpoC2 PE=3 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 6.7e-37
Identity = 75/75 (100.00%), Postives = 75/75 (100.00%), Query Frame = 0

Query: 1   MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 60
           MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL
Sbjct: 259 MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 318

Query: 61  GEAVGIIAGQSIGEP 76
           GEAVGIIAGQSIGEP
Sbjct: 319 GEAVGIIAGQSIGEP 333

BLAST of CmaCh01G009040 vs. NCBI nr
Match: YP_009751980.1 (RNA polymerase beta'' subunit [Cionosicys macranthus] >QIT05221.1 RNA polymerase beta'' subunit [Cionosicys macranthus])

HSP 1 Score: 162.9 bits (411), Expect = 1.4e-36
Identity = 75/75 (100.00%), Postives = 75/75 (100.00%), Query Frame = 0

Query: 1   MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 60
           MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL
Sbjct: 255 MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 314

Query: 61  GEAVGIIAGQSIGEP 76
           GEAVGIIAGQSIGEP
Sbjct: 315 GEAVGIIAGQSIGEP 329

BLAST of CmaCh01G009040 vs. NCBI nr
Match: ALO22570.1 (RpoC2 [Cucurbita pepo subsp. fraterna] >ALO22633.1 RpoC2 [Cucurbita pepo subsp. ovifera] >ALO22679.1 RpoC2 [Cucurbita pepo var. ozarkana] >ALO22739.1 RpoC2 [Cucurbita pepo subsp. pepo] >ALO22839.1 RpoC2 [Cucurbita pepo var. texana])

HSP 1 Score: 162.9 bits (411), Expect = 1.4e-36
Identity = 75/75 (100.00%), Postives = 75/75 (100.00%), Query Frame = 0

Query: 1   MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 60
           MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL
Sbjct: 259 MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 318

Query: 61  GEAVGIIAGQSIGEP 76
           GEAVGIIAGQSIGEP
Sbjct: 319 GEAVGIIAGQSIGEP 333

BLAST of CmaCh01G009040 vs. NCBI nr
Match: YP_009525176.1 (RNA polymerase beta subunit [Hodgsonia macrocarpa] >AXR94570.1 RNA polymerase beta subunit [Hodgsonia macrocarpa] >AXR94656.1 RNA polymerase beta subunit [Hodgsonia macrocarpa] >AXR94740.1 RNA polymerase beta subunit [Hodgsonia macrocarpa] >AXR94825.1 RNA polymerase beta subunit [Hodgsonia macrocarpa])

HSP 1 Score: 162.9 bits (411), Expect = 1.4e-36
Identity = 75/75 (100.00%), Postives = 75/75 (100.00%), Query Frame = 0

Query: 1   MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 60
           MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL
Sbjct: 301 MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 360

Query: 61  GEAVGIIAGQSIGEP 76
           GEAVGIIAGQSIGEP
Sbjct: 361 GEAVGIIAGQSIGEP 375

BLAST of CmaCh01G009040 vs. NCBI nr
Match: YP_009560825.1 (RpoC2 [Trichosanthes kirilowii] >QAB33181.1 RpoC2 [Trichosanthes kirilowii])

HSP 1 Score: 162.9 bits (411), Expect = 1.4e-36
Identity = 75/75 (100.00%), Postives = 75/75 (100.00%), Query Frame = 0

Query: 1   MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 60
           MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL
Sbjct: 255 MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 314

Query: 61  GEAVGIIAGQSIGEP 76
           GEAVGIIAGQSIGEP
Sbjct: 315 GEAVGIIAGQSIGEP 329

BLAST of CmaCh01G009040 vs. NCBI nr
Match: QJD26635.1 (RNA polymerase beta'' subunit [Trichosanthes rosthornii])

HSP 1 Score: 162.9 bits (411), Expect = 1.4e-36
Identity = 75/75 (100.00%), Postives = 75/75 (100.00%), Query Frame = 0

Query: 1   MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 60
           MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL
Sbjct: 259 MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 318

Query: 61  GEAVGIIAGQSIGEP 76
           GEAVGIIAGQSIGEP
Sbjct: 319 GEAVGIIAGQSIGEP 333

BLAST of CmaCh01G009040 vs. TAIR 10
Match: ATCG00170.1 (DNA-directed RNA polymerase family protein )

HSP 1 Score: 144.8 bits (364), Expect = 3.6e-35
Identity = 65/75 (86.67%), Postives = 69/75 (92.00%), Query Frame = 0

Query: 1   MGPRCIGIRNQDIGIGLINRFITFQTQPISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 60
           +G RC+  RNQD+GIGL+NR ITF TQ ISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL
Sbjct: 257 IGSRCVAFRNQDLGIGLVNRLITFGTQSISIRTPFTCRSTSWICRLCYGRSPTHGDLVEL 316

Query: 61  GEAVGIIAGQSIGEP 76
           GEAVGIIAGQSIGEP
Sbjct: 317 GEAVGIIAGQSIGEP 331

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q4VZP32.4e-3998.67DNA-directed RNA polymerase subunit beta'' OS=Cucumis sativus OX=3659 GN=rpoC2 P... [more]
Q49L083.4e-3896.00DNA-directed RNA polymerase subunit beta'' OS=Eucalyptus globulus subsp. globulu... [more]
B1A9252.9e-3794.67DNA-directed RNA polymerase subunit beta'' OS=Carica papaya OX=3649 GN=rpoC2 PE=... [more]
Q8S8Y15.0e-3793.33DNA-directed RNA polymerase subunit beta'' OS=Atropa belladonna OX=33113 GN=rpoC... [more]
Q3C1G95.0e-3793.33DNA-directed RNA polymerase subunit beta'' OS=Nicotiana sylvestris OX=4096 GN=rp... [more]
Match NameE-valueIdentityDescription
A0A6M3RTH86.7e-37100.00DNA-directed RNA polymerase subunit beta'' OS=Trichosanthes kirilowii OX=3677 GN... [more]
A0A6M3S4486.7e-37100.00DNA-directed RNA polymerase subunit beta'' OS=Trichosanthes kirilowii var. japon... [more]
A0A346QNZ06.7e-37100.00DNA-directed RNA polymerase subunit beta'' OS=Hodgsonia macrocarpa OX=2184935 GN... [more]
A0A6H0EPT46.7e-37100.00DNA-directed RNA polymerase subunit beta'' OS=Luffa aegyptiaca OX=3670 GN=rpoC2 ... [more]
A0A514YKS06.7e-37100.00DNA-directed RNA polymerase subunit beta'' OS=Siraitia grosvenorii OX=190515 GN=... [more]
Match NameE-valueIdentityDescription
YP_009751980.11.4e-36100.00RNA polymerase beta'' subunit [Cionosicys macranthus] >QIT05221.1 RNA polymerase... [more]
ALO22570.11.4e-36100.00RpoC2 [Cucurbita pepo subsp. fraterna] >ALO22633.1 RpoC2 [Cucurbita pepo subsp. ... [more]
YP_009525176.11.4e-36100.00RNA polymerase beta subunit [Hodgsonia macrocarpa] >AXR94570.1 RNA polymerase be... [more]
YP_009560825.11.4e-36100.00RpoC2 [Trichosanthes kirilowii] >QAB33181.1 RpoC2 [Trichosanthes kirilowii][more]
QJD26635.11.4e-36100.00RNA polymerase beta'' subunit [Trichosanthes rosthornii][more]
Match NameE-valueIdentityDescription
ATCG00170.13.6e-3586.67DNA-directed RNA polymerase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007081RNA polymerase Rpb1, domain 5PFAMPF04998RNA_pol_Rpb1_5coord: 16..75
e-value: 2.5E-7
score: 30.4
NoneNo IPR availablePANTHERPTHR19376:SF47DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA''coord: 1..79
NoneNo IPR availablePANTHERPTHR19376DNA-DIRECTED RNA POLYMERASEcoord: 1..79
NoneNo IPR availableSUPERFAMILY64484beta and beta-prime subunits of DNA dependent RNA-polymerasecoord: 9..75

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G009040.1CmaCh01G009040.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0009507 chloroplast
molecular_function GO:0003677 DNA binding
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0008270 zinc ion binding