CmaCh01G007790 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh01G007790
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionflavonol synthase/flavanone 3-hydroxylase-like
LocationCma_Chr01: 4207787 .. 4210437 (-)
RNA-Seq ExpressionCmaCh01G007790
SyntenyCmaCh01G007790
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAATGACCAAAGCATGCCTACCAACTGTCATTTTCCATCCTTAGCGGCAGTCTTTTGTTGGGTATAAAATTGCATAAAAAGAAAATGCTTCCACATCACTCTGCAACTTAAGAACTCCTTCGAAAATGGCTAACACAGGCATCCCTACTGTGGACGTTTCTCTGTTCTTAAGTGAAGGTGAAAACGAAGCCAAAAAACAGGCCATTCAAACCATAACTGAAGCTTGTTCAAGCTATGGCTTCTTTCAAATTGTGAACCATGGAATCCCCATTGAATTTCTTAAAGAAGCTCTGCAACTGTCAAAAACATTTTTCCACTACCCTGATGAGATAAAGCTTCAATATAGCCCCAAGCCTGGGGCTCCCCTTCTTGCTGGCTTCAACAAGCAGAAGACAAATTGTGTAGACAAGAATGAATATGTCCTCGTTTTCCCTCCTGGATCCAAATTCAACATCTACCCACAAGAGCCACCACAATTCAAGTGAGTTTTTTGAGATTTGGGTTCTGTTCTTTTGATCTTGCACGTTATGTTGGATTCAAAATGGTTTGGTATGAGTATTATAAACATTTTGGTTGTGGCAGGGAAACGCTGGAAGAGATGTTCCTGAAACTAAGCGATGTGTCCTTGGTTATAGAGAGCATATTGAATGTGTGTTTGGGGCTTCCTCCTGGTTTCCTCAAACAATTCAACAACGACAGAAGCTGGGACTTCATGACTAATCTCTACTATTACCCTGCAGCTGATGTGGGAGAAAATGGCCTCATTCACCATGAAGATGCAAACTGCATCACTTTGGTGATCCAGGATGATGCTGGAGGCCTTCAAGTCCAGAAGGACAGCGAATGGATTCCAGTCACCCCTGTTGAAGGAGCCATTGTTGTGAACGTGGGAGATATAATTCAGGTAAAAGAACACTGTTGTTAAACATTGAAGGAGCCATTGGGCTAGTGAGAGATCTGTTTGTTTGAAAAATTTTGTTTTAATGAAGGTGTTGAGCAACAAGAAATTCAAGAGCGCCACACACAGAGTGGTGAGGCAAAAGGGAAAAGAGAGATATTCTTTTGCATTCTTCCGCAGCTTGCATGGAGACAAATGGGTGGAGCCATTGCCAGAGTTCACTAAAGAAATTGGAGAGAAACCTAAATACAAAGGATTTGAGTTCAATGAATACTTAGCATTGAGATTGAAGAACAAAACCCATCCACCTTCAAGAGTGGAGGATGAGATTAGCATCAAACATTATGAAATCAATTGAAAAATATATGTAAGAACAGAACCAACCACAGAGAAATAATGTAGAAGCACTTCCTGCTGTCTCTATTTGTTGCTTTTACTGTCATGAAAGCTCGAGAGAGCCCTTTAAACTCGCTTGTTGTATTGTCATTTCATTTCATCATCCTACACTATGTTCTGTCTTTATATTAGAAATGTATGAAGAGAGAAAAAAAACAGTGTTTAGTGGACATAGTTCATATTTGTTTCCCTTATCTTTTTATTATTGTTCTTGTTCGGAAGCTTAGGTTGATTTTGGATTACTTTTGGCAGCAAGCTTCACATCATCATCCCTCAAAATGCCAAGTTCCAGAAACACACACTGAGAGAGAGATTCAGGTTGAGGTTGGTATCTCAATTTTCGCTCTTTTCTTGATGAAACTTGTTCATTCTTGACCCATTAGATTGGTGTGGAATAGTGTTAAAGCTTTGCTGAATGTTATGTTTGAGAATTCTGATTAGCCTTGGTGATAATTTAGGCCTCTATCATGTCACTTGTTTGAGCATGTTGGACGTTTATATATGTATGATATACTCGTAGGAGCTTGTTATAGGAGCTAAACACCGTGTATACCCAATTAATGTTTGGATATTCGTTTTATGCTCTCTAGAGAGTATGAAAGGATATATGAATGAACATGAGATGTTGTTTTACGTTTCCTTAGTATCAAGTAGGTATGATTGGGGAGTAACCCCTATTCGTAAACTTTTCTAGAGCGGTTAAGGTCTACTTGGTATGCAAAGTTTAGTTGTCACCCACAAAGGGCCCATGTATGGAGAAACAAGTGGTCGAAGTACTTGTGTGTTTATTAGAGCATGGATTACCTCGTACACACAAGACTTACCCCAAGTTCCATTACAAGGGTAATTTGAATTACGCTCAATTAGTTCAAGGTTGATGATAGATTTAGGAAAACGAAAACATAGAGCTAAGCATATCCGAAATAGAGGGCTTACGTATACACCCCTAGGAGTATACTGGGGTTAGGTATACCCTCCTTTATAAGGTTAGCATAGGCTACATGTCATGATAATGGCTAACGTTAGGGTTACCATATTAGTACATAGTTTAAGAAACCGTCACAAGGTCAGAAGGAAGAAGTTTGCTAAGAGCGTACAAGTACGTTGCTTACAGAGTAGTTTATACTCATTCTTGCTACCCTTTGTTTTTTAGATGGGATATGGACGATTGTTTGATGATACGAGTATACAAGTCGGGCAGTTGTTGGAGGTATCCATAGATCAATTATTTTTCCTTACGTGTCCCTTCTAAGATTCTTTAATGTTATTAACTTCAAAACTCAGGCTATTCTTTTTCAGATTATGCTATTTTATTTCAGATTTAGTGTTGTATTTGAATTTCAAATTCCTTTTATT

mRNA sequence

AAAATGACCAAAGCATGCCTACCAACTGTCATTTTCCATCCTTAGCGGCAGTCTTTTGTTGGGTATAAAATTGCATAAAAAGAAAATGCTTCCACATCACTCTGCAACTTAAGAACTCCTTCGAAAATGGCTAACACAGGCATCCCTACTGTGGACGTTTCTCTGTTCTTAAGTGAAGGTGAAAACGAAGCCAAAAAACAGGCCATTCAAACCATAACTGAAGCTTGTTCAAGCTATGGCTTCTTTCAAATTGTGAACCATGGAATCCCCATTGAATTTCTTAAAGAAGCTCTGCAACTGTCAAAAACATTTTTCCACTACCCTGATGAGATAAAGCTTCAATATAGCCCCAAGCCTGGGGCTCCCCTTCTTGCTGGCTTCAACAAGCAGAAGACAAATTGTGTAGACAAGAATGAATATGTCCTCGTTTTCCCTCCTGGATCCAAATTCAACATCTACCCACAAGAGCCACCACAATTCAAGGAAACGCTGGAAGAGATGTTCCTGAAACTAAGCGATGTGTCCTTGGTTATAGAGAGCATATTGAATGTGTGTTTGGGGCTTCCTCCTGGTTTCCTCAAACAATTCAACAACGACAGAAGCTGGGACTTCATGACTAATCTCTACTATTACCCTGCAGCTGATGTGGGAGAAAATGGCCTCATTCACCATGAAGATGCAAACTGCATCACTTTGGTGATCCAGGATGATGCTGGAGGCCTTCAAGTCCAGAAGGACAGCGAATGGATTCCAGTCACCCCTGTTGAAGGAGCCATTGTTGTGAACGTGGGAGATATAATTCAGGTGTTGAGCAACAAGAAATTCAAGAGCGCCACACACAGAGTGGTGAGGCAAAAGGGAAAAGAGAGATATTCTTTTGCATTCTTCCGCAGCTTGCATGGAGACAAATGGGTGGAGCCATTGCCAGAGTTCACTAAAGAAATTGGAGAGAAACCTAAATACAAAGGATTTGAGTTCAATGAATACTTAGCATTGAGATTGAAGAACAAAACCCATCCACCTTCAAGAGTGGAGGATGAGATTAGCATCAAACATTATGAAATCAATTGAAAAATATATGTAAGAACAGAACCAACCACAGAGAAATAATGTAGAAGCACTTCCTGCTGTCTCTATTTGTTGCTTTTACTGTCATGAAAGCTCGAGAGAGCCCTTTAAACTCGCTTGTTGTATTGTCATTTCATTTCATCATCCTACACTATGTTCTGTCTTTATATTAGAAATGTATGAAGAGAGAAAAAAAACAGTGTTTAGTGGACATAGTTCATATTTGTTTCCCTTATCTTTTTATTATTGTTCTTGTTCGGAAGCTTAGGTTGATTTTGGATTACTTTTGGCAGCAAGCTTCACATCATCATCCCTCAAAATGCCAAGTTCCAGAAACACACACTGAGAGAGAGATTCAGGTTGAGATGGGATATGGACGATTGTTTGATGATACGAGTATACAAGTCGGGCAGTTGTTGGAGGTATCCATAGATCAATTATTTTTCCTTACGTGTCCCTTCTAAGATTCTTTAATGTTATTAACTTCAAAACTCAGGCTATTCTTTTTCAGATTATGCTATTTTATTTCAGATTTAGTGTTGTATTTGAATTTCAAATTCCTTTTATT

Coding sequence (CDS)

ATGGCTAACACAGGCATCCCTACTGTGGACGTTTCTCTGTTCTTAAGTGAAGGTGAAAACGAAGCCAAAAAACAGGCCATTCAAACCATAACTGAAGCTTGTTCAAGCTATGGCTTCTTTCAAATTGTGAACCATGGAATCCCCATTGAATTTCTTAAAGAAGCTCTGCAACTGTCAAAAACATTTTTCCACTACCCTGATGAGATAAAGCTTCAATATAGCCCCAAGCCTGGGGCTCCCCTTCTTGCTGGCTTCAACAAGCAGAAGACAAATTGTGTAGACAAGAATGAATATGTCCTCGTTTTCCCTCCTGGATCCAAATTCAACATCTACCCACAAGAGCCACCACAATTCAAGGAAACGCTGGAAGAGATGTTCCTGAAACTAAGCGATGTGTCCTTGGTTATAGAGAGCATATTGAATGTGTGTTTGGGGCTTCCTCCTGGTTTCCTCAAACAATTCAACAACGACAGAAGCTGGGACTTCATGACTAATCTCTACTATTACCCTGCAGCTGATGTGGGAGAAAATGGCCTCATTCACCATGAAGATGCAAACTGCATCACTTTGGTGATCCAGGATGATGCTGGAGGCCTTCAAGTCCAGAAGGACAGCGAATGGATTCCAGTCACCCCTGTTGAAGGAGCCATTGTTGTGAACGTGGGAGATATAATTCAGGTGTTGAGCAACAAGAAATTCAAGAGCGCCACACACAGAGTGGTGAGGCAAAAGGGAAAAGAGAGATATTCTTTTGCATTCTTCCGCAGCTTGCATGGAGACAAATGGGTGGAGCCATTGCCAGAGTTCACTAAAGAAATTGGAGAGAAACCTAAATACAAAGGATTTGAGTTCAATGAATACTTAGCATTGAGATTGAAGAACAAAACCCATCCACCTTCAAGAGTGGAGGATGAGATTAGCATCAAACATTATGAAATCAATTGA

Protein sequence

MANTGIPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSKTFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLVFPPGSKFNIYPQEPPQFKETLEEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVGENGLIHHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKKFKSATHRVVRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALRLKNKTHPPSRVEDEISIKHYEIN
Homology
BLAST of CmaCh01G007790 vs. ExPASy Swiss-Prot
Match: Q9ZWQ9 (Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 5.1e-35
Identity = 89/296 (30.07%), Postives = 150/296 (50.68%), Query Frame = 0

Query: 6   IPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSKTFFHY 65
           IPT+D+       ++  + + +++I EA   +G FQ+ NHGIP + + +   + K FF  
Sbjct: 43  IPTIDL-------DDPVQDRLVRSIAEASREWGIFQVTNHGIPSDLICKLQAVGKEFFEL 102

Query: 66  PDEIKLQYSPKPGAPLLAGF-NKQKTNCVDKNEYV-----LVFPPGS-KFNIYPQEPPQF 125
           P E K  YS    A  + G+  K +     K  +V      V+PP S  +  +P+ PP +
Sbjct: 103 PQEEKEVYSRPADAKDVQGYGTKLQKEVEGKKSWVDHLFHRVWPPSSINYRFWPKNPPSY 162

Query: 126 KETLEEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVGE-- 185
           +   EE    + +V   + + L++ LG+  G LK+       ++M  + YYP     +  
Sbjct: 163 RAVNEEYAKYMREVVDKLFTYLSLGLGVEGGVLKEAAGGDDIEYMLKINYYPPCPRPDLA 222

Query: 186 NGLIHHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKKFKSA 245
            G++ H D + +T+++ ++  GLQV KD  WI    +  A+V+++GD I++LSN K+K+ 
Sbjct: 223 LGVVAHTDLSALTVLVPNEVPGLQVFKDDRWIDAKYIPNALVIHIGDQIEILSNGKYKAV 282

Query: 246 THRVVRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALRL 293
            HR    K K R S+  F     D  V PLP+   +    PKYK  +F +Y   +L
Sbjct: 283 LHRTTVNKDKTRMSWPVFLEPPADTVVGPLPQLVDD-ENPPKYKAKKFKDYSYCKL 330

BLAST of CmaCh01G007790 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 6.6e-35
Identity = 109/315 (34.60%), Postives = 153/315 (48.57%), Query Frame = 0

Query: 4   TGIPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSKTFF 63
           T IP +D+    SE E  +    +  I+EAC  +GFFQ+VNHG+  E +  A +  + FF
Sbjct: 60  TNIPIIDLEGLFSE-EGLSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFF 119

Query: 64  HYPDEIKLQYSPKPGAPLLAG--FNKQKTNCVDKNEYVL--VFPPGSK-FNIYPQEPPQF 123
           H P   K  YS  P      G     +K   +D ++Y    + P   K FN +P  PP  
Sbjct: 120 HMPVNAKETYSNSPRTYEGYGSRLGVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTI 179

Query: 124 KETLEEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVGE-- 183
           +E ++E   +L  +S  I  +L+  LGL     ++     +      + YYP     E  
Sbjct: 180 REVIDEYGEELVKLSGRIMRVLSTNLGLKEDKFQEAFGGENIGACLRVNYYPKCPRPELA 239

Query: 184 NGLIHHEDANCITLVIQDD-AGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKKFKS 243
            GL  H D   +T+++ DD   GLQV+KD  WI V P   A +VN+GD IQ+LSN  +KS
Sbjct: 240 LGLSPHSDPGGMTILLPDDQVFGLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKS 299

Query: 244 ATHRVVRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALRLKNK 303
             HRV+    KER S AFF +   D  ++PL E        P Y    F++Y   RL  +
Sbjct: 300 VEHRVIVNSDKERVSLAFFYNPKSDIPIQPLQELV-STHNPPLYPPMTFDQY---RLFIR 359

Query: 304 THPP---SRVEDEIS 308
           T  P   S VE  IS
Sbjct: 360 TQGPQGKSHVESHIS 369

BLAST of CmaCh01G007790 vs. ExPASy Swiss-Prot
Match: O04395 (Flavonol synthase/flavanone 3-hydroxylase (Fragment) OS=Matthiola incana OX=3724 PE=2 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 8.6e-35
Identity = 101/304 (33.22%), Postives = 157/304 (51.64%), Query Frame = 0

Query: 6   IPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSKTFFHY 65
           +P VD+S          ++   +T+ +A   +G FQ+VNHGIP E ++   ++ + FF  
Sbjct: 2   VPVVDLSC-------PDEELVARTVVKASEDWGVFQVVNHGIPTELIQRLQKVGREFFEL 61

Query: 66  PDEIKLQYSPKPGAPLLAGFN-------KQKTNCVDKNEYVLVFPPGS-KFNIYPQEPPQ 125
           P+  K   + + G+  + G+        K++   VD+  Y+  +PP S  +  +P+ PP 
Sbjct: 62  PEAEKRSCAREAGS--VEGYGRRIELDIKKRKGIVDQ-IYLSTWPPSSVNYRYWPKSPPD 121

Query: 126 FKETLEEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYY--YPAADVG 185
           ++E  EE    +  +S  I   L+  LGL    +K+ N    W  M   +Y  YP +D  
Sbjct: 122 YREVNEEYARHVKTLSEKIMEWLSEGLGLGREAIKEVNG--CWYVMNINHYPPYPHSD-S 181

Query: 186 ENGLIHHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKKFKS 245
            NGL  H D N +TL+I ++  GLQV KD  WI V  +  AI+VN+GD I +LSN K+K+
Sbjct: 182 FNGLEPHTDINGLTLIITNEIPGLQVFKDDHWIEVEYIPSAIIVNIGDQIMMLSNGKYKN 241

Query: 246 ATHRVVRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALR---L 297
             H+    K K R S+    S   D  V PLPE T E  + PK+K   + +Y+  +   L
Sbjct: 242 VLHKTTVDKEKTRMSWPVLVSPTYDMVVGPLPELTSE-DDPPKFKPIAYKDYVHNKITFL 291

BLAST of CmaCh01G007790 vs. ExPASy Swiss-Prot
Match: Q00985 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.1e-34
Identity = 90/289 (31.14%), Postives = 151/289 (52.25%), Query Frame = 0

Query: 7   PTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSKTFFHYP 66
           P VD+SL       E +   ++ I +AC ++GFF++VNHG+  E L    +++K   HY 
Sbjct: 5   PVVDLSLV----NGEERAATLEKINDACENWGFFELVNHGMSTELLDTVEKMTKD--HYK 64

Query: 67  DEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLVFPPGSKFNIYPQEPPQFKETLEEMF 126
             ++ ++     A  L     +  +   ++ + L   P S  +  P    ++++T++E  
Sbjct: 65  KTMEQRFKEMVAAKGLDDVQSEIHDLDWESTFFLRHLPSSNISEIPDLEEEYRKTMKEFA 124

Query: 127 LKLSDVSLVIESILNVCLGLPPGFLKQ-FNNDRSWDFMTNLYYYPAADVGE--NGLIHHE 186
           ++L  ++  +  +L   LGL  G+LK+ F   +  +F T +  YP     +   GL  H 
Sbjct: 125 VELEKLAEKLLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHS 184

Query: 187 DANCITLVIQDD-AGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKKFKSATHRVVR 246
           DA  I L+ QDD   GLQ+ KD EW+ V P+  +IV+N+GD I+V++N K+KS  HRV+ 
Sbjct: 185 DAGGIILLFQDDKVSGLQLLKDGEWVDVPPMHHSIVINLGDQIEVITNGKYKSVMHRVIA 244

Query: 247 QKGKERYSFAFFRSLHGDKWVEPLPE-FTKEIGEKPKYKGFEFNEYLAL 291
           Q    R S A F +   D ++ P P    K+  + P Y  F F++Y+ L
Sbjct: 245 QSDGTRMSIASFYNPGNDSFISPAPAVLEKKTEDAPTYPKFVFDDYMKL 287

BLAST of CmaCh01G007790 vs. ExPASy Swiss-Prot
Match: Q9SRM3 (Jasmonate-induced oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=JOX1 PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 5.6e-34
Identity = 107/318 (33.65%), Postives = 157/318 (49.37%), Query Frame = 0

Query: 1   MANTGIPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSK 60
           +A+  IP +D+    S  E++ K+     I+EAC  +GFFQ++NHG+  E +  A +  K
Sbjct: 90  VADINIPIIDLDSLFSGNEDDKKR-----ISEACREWGFFQVINHGVKPELMDAARETWK 149

Query: 61  TFFHYPDEIKLQYSPKPGAPLLAG--FNKQKTNCVDKNE-YVLVFPPGS--KFNIYPQEP 120
           +FF+ P E K  YS  P      G     +K   +D N+ Y L F P +   FN +P  P
Sbjct: 150 SFFNLPVEAKEVYSNSPRTYEGYGSRLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLP 209

Query: 121 PQFKETLEEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVG 180
              +E  +E   +L  +   + +IL+  LGL    L++            + YYP     
Sbjct: 210 SNIREMNDEYGKELVKLGGRLMTILSSNLGLRAEQLQEAFGGEDVGACLRVNYYPKCPQP 269

Query: 181 E--NGLIHHEDANCITLVIQDD-AGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKK 240
           E   GL  H D   +T+++ DD   GLQV+    WI V P+  A +VN+GD IQ+LSN K
Sbjct: 270 ELALGLSPHSDPGGMTILLPDDQVVGLQVRHGDTWITVNPLRHAFIVNIGDQIQILSNSK 329

Query: 241 FKSATHRVVRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALRL 300
           +KS  HRV+    KER S AFF +   D  ++P+ +        P Y    F++Y   RL
Sbjct: 330 YKSVEHRVIVNSEKERVSLAFFYNPKSDIPIQPMQQLVTST-MPPLYPPMTFDQY---RL 389

Query: 301 KNKTHPP---SRVEDEIS 308
             +T  P   S VE  IS
Sbjct: 390 FIRTQGPRGKSHVESHIS 398

BLAST of CmaCh01G007790 vs. ExPASy TrEMBL
Match: O23763 (Gibberellin 7-oxidase OS=Cucurbita maxima OX=3661 GN=LOC111493375 PE=2 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 4.4e-183
Identity = 314/314 (100.00%), Postives = 314/314 (100.00%), Query Frame = 0

Query: 1   MANTGIPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSK 60
           MANTGIPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSK
Sbjct: 1   MANTGIPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSK 60

Query: 61  TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLVFPPGSKFNIYPQEPPQFKE 120
           TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLVFPPGSKFNIYPQEPPQFKE
Sbjct: 61  TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLVFPPGSKFNIYPQEPPQFKE 120

Query: 121 TLEEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVGENGLI 180
           TLEEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVGENGLI
Sbjct: 121 TLEEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVGENGLI 180

Query: 181 HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKKFKSATHRV 240
           HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKKFKSATHRV
Sbjct: 181 HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKKFKSATHRV 240

Query: 241 VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALRLKNKTHPPS 300
           VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALRLKNKTHPPS
Sbjct: 241 VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALRLKNKTHPPS 300

Query: 301 RVEDEISIKHYEIN 315
           RVEDEISIKHYEIN
Sbjct: 301 RVEDEISIKHYEIN 314

BLAST of CmaCh01G007790 vs. ExPASy TrEMBL
Match: A0A6J1GB31 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111452556 PE=3 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 2.3e-179
Identity = 304/314 (96.82%), Postives = 312/314 (99.36%), Query Frame = 0

Query: 1   MANTGIPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSK 60
           MANTGIPTVDVSLFLSEGENEAKKQA+QTITEACSSYGFFQIVNHGIP+EFLKEALQLSK
Sbjct: 1   MANTGIPTVDVSLFLSEGENEAKKQALQTITEACSSYGFFQIVNHGIPVEFLKEALQLSK 60

Query: 61  TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLVFPPGSKFNIYPQEPPQFKE 120
           TFFHYPDEIKLQYS KPGAPLLAGFNKQKTNCVDKNEYVL+FPPGSKFNIYPQEPPQFKE
Sbjct: 61  TFFHYPDEIKLQYSSKPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKE 120

Query: 121 TLEEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVGENGLI 180
           TLEEMFLKL+DVSLV+ESILN CLGLPPGFLKQFNNDRSWDFMTNLYY+PAADVGENGLI
Sbjct: 121 TLEEMFLKLNDVSLVVESILNECLGLPPGFLKQFNNDRSWDFMTNLYYFPAADVGENGLI 180

Query: 181 HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKKFKSATHRV 240
           HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGD+IQVLSNKKFKSATHRV
Sbjct: 181 HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRV 240

Query: 241 VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALRLKNKTHPPS 300
           VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKY+GFEFNEYLALRLKNKTHPPS
Sbjct: 241 VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTHPPS 300

Query: 301 RVEDEISIKHYEIN 315
           RVEDEISIKHYEIN
Sbjct: 301 RVEDEISIKHYEIN 314

BLAST of CmaCh01G007790 vs. ExPASy TrEMBL
Match: A0A1S3B2M0 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103485283 PE=3 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 3.6e-153
Identity = 254/312 (81.41%), Postives = 287/312 (91.99%), Query Frame = 0

Query: 3   NTGIPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSKTF 62
           NTGIPTVD+S+F +E ENEAKK+A +TI +ACSSYGFFQIVNHGIP+EFLKE+L++S+TF
Sbjct: 17  NTGIPTVDLSVFSTEDENEAKKKAFETIFQACSSYGFFQIVNHGIPVEFLKESLEVSRTF 76

Query: 63  FHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLVFPPGSKFNIYPQEPPQFKETL 122
           FHYPD+IKL+YSPKPGAPLLAGFNKQK NCVDKNEYVL+FPPGS FNIYPQEPPQFKE L
Sbjct: 77  FHYPDDIKLKYSPKPGAPLLAGFNKQKKNCVDKNEYVLMFPPGSNFNIYPQEPPQFKEQL 136

Query: 123 EEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVGENGLIHH 182
           EE+F KLS V L++ESI+N  LGLPP FLKQ+NNDRSWDFMT LYY+ A + GENGL HH
Sbjct: 137 EELFKKLSKVCLLLESIVNEGLGLPPDFLKQYNNDRSWDFMTTLYYFSATEEGENGLTHH 196

Query: 183 EDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKKFKSATHRVVR 242
           EDANCITLVIQDD GGLQV+KD EWIPV PVEGAIVVN+GD+IQVLSNKKFKSATHRVVR
Sbjct: 197 EDANCITLVIQDDTGGLQVRKDGEWIPVVPVEGAIVVNIGDVIQVLSNKKFKSATHRVVR 256

Query: 243 QKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALRLKNKTHPPSRV 302
           QKGKER+S+AFF +LHGDKWVEPLP+FT+EIGEKPKYKGF+F +YLA+RLKNKTHPPSRV
Sbjct: 257 QKGKERFSYAFFHNLHGDKWVEPLPKFTEEIGEKPKYKGFQFKDYLAMRLKNKTHPPSRV 316

Query: 303 EDEISIKHYEIN 315
           EDEI I HYEI+
Sbjct: 317 EDEIRITHYEIS 328

BLAST of CmaCh01G007790 vs. ExPASy TrEMBL
Match: S0BE22 (GA 7-oxidase OS=Cucumis sativus OX=3659 GN=GA7ox1 PE=2 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 2.9e-150
Identity = 250/312 (80.13%), Postives = 282/312 (90.38%), Query Frame = 0

Query: 3   NTGIPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSKTF 62
           +TGIPTVD+S+F ++ E EAKK+A +TI +ACSSYGFFQIVNHG+PIEFL+EAL+LS+TF
Sbjct: 17  DTGIPTVDLSVFSAQNETEAKKKAFETIYQACSSYGFFQIVNHGVPIEFLEEALELSRTF 76

Query: 63  FHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLVFPPGSKFNIYPQEPPQFKETL 122
           FHYPD+IKL+YS KPGAPLLAGFNKQK NCVDKNEYVLVFPPGS +NIYPQEPPQFKE L
Sbjct: 77  FHYPDDIKLKYSSKPGAPLLAGFNKQKKNCVDKNEYVLVFPPGSNYNIYPQEPPQFKELL 136

Query: 123 EEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVGENGLIHH 182
           EEMF KLS V L++ESI+N  LGLPP FLKQ+NNDRSWDFMT LYY+ A + GENGL HH
Sbjct: 137 EEMFKKLSKVCLLLESIVNESLGLPPDFLKQYNNDRSWDFMTTLYYFSATEEGENGLTHH 196

Query: 183 EDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKKFKSATHRVVR 242
           ED NCITLV QDD GGLQV+KD EWIPV PVEGAIVVN+GD+IQVLSNKKFKSATHRVVR
Sbjct: 197 EDGNCITLVFQDDTGGLQVRKDGEWIPVVPVEGAIVVNIGDVIQVLSNKKFKSATHRVVR 256

Query: 243 QKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALRLKNKTHPPSRV 302
           QKGKER+S+AFF +LHGDKWVEPLP+FT+EIGEKPKYKGF+F +Y ALRLKNKTHPPSRV
Sbjct: 257 QKGKERFSYAFFHNLHGDKWVEPLPQFTEEIGEKPKYKGFQFKDYQALRLKNKTHPPSRV 316

Query: 303 EDEISIKHYEIN 315
           EDEI I HYEI+
Sbjct: 317 EDEIRITHYEIS 328

BLAST of CmaCh01G007790 vs. ExPASy TrEMBL
Match: A0A5D3BJI5 (Flavonol synthase/flavanone 3-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00770 PE=4 SV=1)

HSP 1 Score: 540.4 bits (1391), Expect = 4.9e-150
Identity = 249/304 (81.91%), Postives = 281/304 (92.43%), Query Frame = 0

Query: 3   NTGIPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSKTF 62
           NTGIPTVD+S+F +E ENEAKK+A +TI +ACSSYGFFQIVNHGIP+EFLKE+L++S+TF
Sbjct: 4   NTGIPTVDLSVFSTEDENEAKKKAFETIFQACSSYGFFQIVNHGIPVEFLKESLEVSRTF 63

Query: 63  FHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLVFPPGSKFNIYPQEPPQFKETL 122
           FHYPD+IKL+YSPKPGAPLLAGFNKQK NCVDKNEYVL+FPPGS FNIYPQEPPQFKE L
Sbjct: 64  FHYPDDIKLKYSPKPGAPLLAGFNKQKKNCVDKNEYVLMFPPGSNFNIYPQEPPQFKEQL 123

Query: 123 EEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVGENGLIHH 182
           EE+F KLS V L++ESI+N  LGLPP FLKQ+NNDRSWDFMT LYY+ A + GENGL HH
Sbjct: 124 EELFKKLSKVCLLLESIVNEGLGLPPDFLKQYNNDRSWDFMTTLYYFSATEEGENGLTHH 183

Query: 183 EDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKKFKSATHRVVR 242
           EDANCITLVIQDD GGLQV+KD EWIPV PVEGAIVVN+GD+IQVLSNKKFKSATHRVVR
Sbjct: 184 EDANCITLVIQDDTGGLQVRKDGEWIPVVPVEGAIVVNIGDVIQVLSNKKFKSATHRVVR 243

Query: 243 QKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALRLKNKTHPPSRV 302
           QKGKER+S+AFF +LHGDKWVEPLP+FT+EIGEKPKYKGF+F +YLA+RLKNKTHPPSRV
Sbjct: 244 QKGKERFSYAFFHNLHGDKWVEPLPKFTEEIGEKPKYKGFQFKDYLAMRLKNKTHPPSRV 303

Query: 303 EDEI 307
           EDEI
Sbjct: 304 EDEI 307

BLAST of CmaCh01G007790 vs. NCBI nr
Match: XP_022998829.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita maxima] >AAB64346.1 gibberellin 7-oxidase [Cucurbita maxima])

HSP 1 Score: 650.2 bits (1676), Expect = 9.1e-183
Identity = 314/314 (100.00%), Postives = 314/314 (100.00%), Query Frame = 0

Query: 1   MANTGIPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSK 60
           MANTGIPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSK
Sbjct: 1   MANTGIPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSK 60

Query: 61  TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLVFPPGSKFNIYPQEPPQFKE 120
           TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLVFPPGSKFNIYPQEPPQFKE
Sbjct: 61  TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLVFPPGSKFNIYPQEPPQFKE 120

Query: 121 TLEEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVGENGLI 180
           TLEEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVGENGLI
Sbjct: 121 TLEEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVGENGLI 180

Query: 181 HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKKFKSATHRV 240
           HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKKFKSATHRV
Sbjct: 181 HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKKFKSATHRV 240

Query: 241 VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALRLKNKTHPPS 300
           VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALRLKNKTHPPS
Sbjct: 241 VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALRLKNKTHPPS 300

Query: 301 RVEDEISIKHYEIN 315
           RVEDEISIKHYEIN
Sbjct: 301 RVEDEISIKHYEIN 314

BLAST of CmaCh01G007790 vs. NCBI nr
Match: KAG6607440.1 (hypothetical protein SDJN03_00782, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037097.1 hypothetical protein SDJN02_00718 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 638.3 bits (1645), Expect = 3.6e-179
Identity = 305/314 (97.13%), Postives = 312/314 (99.36%), Query Frame = 0

Query: 1   MANTGIPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSK 60
           MANTGIPTVDVSLFLSEGENEAKKQA+QTITEACSSYGFFQIVNHGIP+EFLKEALQLSK
Sbjct: 1   MANTGIPTVDVSLFLSEGENEAKKQALQTITEACSSYGFFQIVNHGIPVEFLKEALQLSK 60

Query: 61  TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLVFPPGSKFNIYPQEPPQFKE 120
           TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVL+FPPGSKFNIYPQEPPQFKE
Sbjct: 61  TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKE 120

Query: 121 TLEEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVGENGLI 180
           TLEEMFLKL+DVSLVIESILN CLGL PGFLKQFNNDRSWDFMTNLYY+PAADVGENGLI
Sbjct: 121 TLEEMFLKLNDVSLVIESILNECLGLAPGFLKQFNNDRSWDFMTNLYYFPAADVGENGLI 180

Query: 181 HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKKFKSATHRV 240
           HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGD+IQVLSNKKFKSATHRV
Sbjct: 181 HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRV 240

Query: 241 VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALRLKNKTHPPS 300
           VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKY+GFEFNEYLALRLKNKTHPPS
Sbjct: 241 VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTHPPS 300

Query: 301 RVEDEISIKHYEIN 315
           RVEDEISIKHYEIN
Sbjct: 301 RVEDEISIKHYEIN 314

BLAST of CmaCh01G007790 vs. NCBI nr
Match: XP_022949101.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 637.9 bits (1644), Expect = 4.7e-179
Identity = 304/314 (96.82%), Postives = 312/314 (99.36%), Query Frame = 0

Query: 1   MANTGIPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSK 60
           MANTGIPTVDVSLFLSEGENEAKKQA+QTITEACSSYGFFQIVNHGIP+EFLKEALQLSK
Sbjct: 1   MANTGIPTVDVSLFLSEGENEAKKQALQTITEACSSYGFFQIVNHGIPVEFLKEALQLSK 60

Query: 61  TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLVFPPGSKFNIYPQEPPQFKE 120
           TFFHYPDEIKLQYS KPGAPLLAGFNKQKTNCVDKNEYVL+FPPGSKFNIYPQEPPQFKE
Sbjct: 61  TFFHYPDEIKLQYSSKPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKE 120

Query: 121 TLEEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVGENGLI 180
           TLEEMFLKL+DVSLV+ESILN CLGLPPGFLKQFNNDRSWDFMTNLYY+PAADVGENGLI
Sbjct: 121 TLEEMFLKLNDVSLVVESILNECLGLPPGFLKQFNNDRSWDFMTNLYYFPAADVGENGLI 180

Query: 181 HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKKFKSATHRV 240
           HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGD+IQVLSNKKFKSATHRV
Sbjct: 181 HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRV 240

Query: 241 VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALRLKNKTHPPS 300
           VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKY+GFEFNEYLALRLKNKTHPPS
Sbjct: 241 VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTHPPS 300

Query: 301 RVEDEISIKHYEIN 315
           RVEDEISIKHYEIN
Sbjct: 301 RVEDEISIKHYEIN 314

BLAST of CmaCh01G007790 vs. NCBI nr
Match: XP_023524991.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 637.1 bits (1642), Expect = 8.0e-179
Identity = 304/314 (96.82%), Postives = 312/314 (99.36%), Query Frame = 0

Query: 1   MANTGIPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSK 60
           MANTGIPTVDVSLFLSEGENEAKKQA+QTIT+ACSSYGFFQIVNHGIP+EFLKEALQLSK
Sbjct: 1   MANTGIPTVDVSLFLSEGENEAKKQALQTITDACSSYGFFQIVNHGIPVEFLKEALQLSK 60

Query: 61  TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLVFPPGSKFNIYPQEPPQFKE 120
           TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVL+FPPGSKFNIYPQEPPQFKE
Sbjct: 61  TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKE 120

Query: 121 TLEEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVGENGLI 180
           TLEEMFLKL+DVSLVIESILN CLGL PGFLKQFNNDRSWDFMTNLYY+PAADVGENGLI
Sbjct: 121 TLEEMFLKLNDVSLVIESILNECLGLAPGFLKQFNNDRSWDFMTNLYYFPAADVGENGLI 180

Query: 181 HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKKFKSATHRV 240
           HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGD+IQVLSNKKFKSATHRV
Sbjct: 181 HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRV 240

Query: 241 VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALRLKNKTHPPS 300
           VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKY+GFEFNEYLALRLKNKTHPPS
Sbjct: 241 VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTHPPS 300

Query: 301 RVEDEISIKHYEIN 315
           RVEDEISIKHYEIN
Sbjct: 301 RVEDEISIKHYEIN 314

BLAST of CmaCh01G007790 vs. NCBI nr
Match: XP_008441091.1 (PREDICTED: flavonol synthase/flavanone 3-hydroxylase-like [Cucumis melo])

HSP 1 Score: 550.8 bits (1418), Expect = 7.5e-153
Identity = 254/312 (81.41%), Postives = 287/312 (91.99%), Query Frame = 0

Query: 3   NTGIPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSKTF 62
           NTGIPTVD+S+F +E ENEAKK+A +TI +ACSSYGFFQIVNHGIP+EFLKE+L++S+TF
Sbjct: 17  NTGIPTVDLSVFSTEDENEAKKKAFETIFQACSSYGFFQIVNHGIPVEFLKESLEVSRTF 76

Query: 63  FHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLVFPPGSKFNIYPQEPPQFKETL 122
           FHYPD+IKL+YSPKPGAPLLAGFNKQK NCVDKNEYVL+FPPGS FNIYPQEPPQFKE L
Sbjct: 77  FHYPDDIKLKYSPKPGAPLLAGFNKQKKNCVDKNEYVLMFPPGSNFNIYPQEPPQFKEQL 136

Query: 123 EEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVGENGLIHH 182
           EE+F KLS V L++ESI+N  LGLPP FLKQ+NNDRSWDFMT LYY+ A + GENGL HH
Sbjct: 137 EELFKKLSKVCLLLESIVNEGLGLPPDFLKQYNNDRSWDFMTTLYYFSATEEGENGLTHH 196

Query: 183 EDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKKFKSATHRVVR 242
           EDANCITLVIQDD GGLQV+KD EWIPV PVEGAIVVN+GD+IQVLSNKKFKSATHRVVR
Sbjct: 197 EDANCITLVIQDDTGGLQVRKDGEWIPVVPVEGAIVVNIGDVIQVLSNKKFKSATHRVVR 256

Query: 243 QKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALRLKNKTHPPSRV 302
           QKGKER+S+AFF +LHGDKWVEPLP+FT+EIGEKPKYKGF+F +YLA+RLKNKTHPPSRV
Sbjct: 257 QKGKERFSYAFFHNLHGDKWVEPLPKFTEEIGEKPKYKGFQFKDYLAMRLKNKTHPPSRV 316

Query: 303 EDEISIKHYEIN 315
           EDEI I HYEI+
Sbjct: 317 EDEIRITHYEIS 328

BLAST of CmaCh01G007790 vs. TAIR 10
Match: AT5G05600.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 149.4 bits (376), Expect = 4.7e-36
Identity = 109/315 (34.60%), Postives = 153/315 (48.57%), Query Frame = 0

Query: 4   TGIPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSKTFF 63
           T IP +D+    SE E  +    +  I+EAC  +GFFQ+VNHG+  E +  A +  + FF
Sbjct: 60  TNIPIIDLEGLFSE-EGLSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFF 119

Query: 64  HYPDEIKLQYSPKPGAPLLAG--FNKQKTNCVDKNEYVL--VFPPGSK-FNIYPQEPPQF 123
           H P   K  YS  P      G     +K   +D ++Y    + P   K FN +P  PP  
Sbjct: 120 HMPVNAKETYSNSPRTYEGYGSRLGVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTI 179

Query: 124 KETLEEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVGE-- 183
           +E ++E   +L  +S  I  +L+  LGL     ++     +      + YYP     E  
Sbjct: 180 REVIDEYGEELVKLSGRIMRVLSTNLGLKEDKFQEAFGGENIGACLRVNYYPKCPRPELA 239

Query: 184 NGLIHHEDANCITLVIQDD-AGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKKFKS 243
            GL  H D   +T+++ DD   GLQV+KD  WI V P   A +VN+GD IQ+LSN  +KS
Sbjct: 240 LGLSPHSDPGGMTILLPDDQVFGLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKS 299

Query: 244 ATHRVVRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALRLKNK 303
             HRV+    KER S AFF +   D  ++PL E        P Y    F++Y   RL  +
Sbjct: 300 VEHRVIVNSDKERVSLAFFYNPKSDIPIQPLQELV-STHNPPLYPPMTFDQY---RLFIR 359

Query: 304 THPP---SRVEDEIS 308
           T  P   S VE  IS
Sbjct: 360 TQGPQGKSHVESHIS 369

BLAST of CmaCh01G007790 vs. TAIR 10
Match: AT3G19010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 147.1 bits (370), Expect = 2.3e-35
Identity = 94/305 (30.82%), Postives = 149/305 (48.85%), Query Frame = 0

Query: 6   IPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSKTFFHY 65
           IP +D+S      + E  +  I  I +AC  +GFFQ++NHG+P +  +   +  K FF  
Sbjct: 28  IPVIDLSRL---DDPEDVQNVISEIGDACEKWGFFQVLNHGVPSDARQRVEKTVKMFFDL 87

Query: 66  PDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYV-------LVFPPGSK---------FN 125
           P E K++       P+     +   N  D  E         +V P  +          +N
Sbjct: 88  PMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEVFDIYFKDPMVIPSTTDPEDEGLRLVYN 147

Query: 126 IYPQEPPQFKETLEEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLY-Y 185
            +PQ P  F+E  E        ++  +  ++++ LGLP      +  ++   F  N Y  
Sbjct: 148 KWPQSPSDFREACEVYARHAEKLAFKLLELISLSLGLPKERFHDYFKEQMSFFRINRYPP 207

Query: 186 YPAADVGENGLIHHEDANCITLVIQDDAGGLQVQK--DSEWIPVTPVEGAIVVNVGDIIQ 245
            P  D+   G+ HH+DA+ I+L+ QDD GGLQV +  D  W P+ PV  A+V+N+G+ ++
Sbjct: 208 CPRPDLA-LGVGHHKDADVISLLAQDDVGGLQVSRRSDGVWFPIRPVPNALVINIGNCME 267

Query: 246 VLSNKKFKSATHRVVRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNE 292
           + +N K+ SA HRVV    +ERYS  FF     D  V+PL E        PKYKG+++ +
Sbjct: 268 IWTNDKYWSAEHRVVVNTTRERYSIPFFLLPSHDVEVKPLEELVSP-ENPPKYKGYKWGK 327

BLAST of CmaCh01G007790 vs. TAIR 10
Match: AT3G11180.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 146.4 bits (368), Expect = 4.0e-35
Identity = 107/318 (33.65%), Postives = 157/318 (49.37%), Query Frame = 0

Query: 1   MANTGIPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSK 60
           +A+  IP +D+    S  E++ K+     I+EAC  +GFFQ++NHG+  E +  A +  K
Sbjct: 90  VADINIPIIDLDSLFSGNEDDKKR-----ISEACREWGFFQVINHGVKPELMDAARETWK 149

Query: 61  TFFHYPDEIKLQYSPKPGAPLLAG--FNKQKTNCVDKNE-YVLVFPPGS--KFNIYPQEP 120
           +FF+ P E K  YS  P      G     +K   +D N+ Y L F P +   FN +P  P
Sbjct: 150 SFFNLPVEAKEVYSNSPRTYEGYGSRLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLP 209

Query: 121 PQFKETLEEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVG 180
              +E  +E   +L  +   + +IL+  LGL    L++            + YYP     
Sbjct: 210 SNIREMNDEYGKELVKLGGRLMTILSSNLGLRAEQLQEAFGGEDVGACLRVNYYPKCPQP 269

Query: 181 E--NGLIHHEDANCITLVIQDD-AGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKK 240
           E   GL  H D   +T+++ DD   GLQV+    WI V P+  A +VN+GD IQ+LSN K
Sbjct: 270 ELALGLSPHSDPGGMTILLPDDQVVGLQVRHGDTWITVNPLRHAFIVNIGDQIQILSNSK 329

Query: 241 FKSATHRVVRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALRL 300
           +KS  HRV+    KER S AFF +   D  ++P+ +        P Y    F++Y   RL
Sbjct: 330 YKSVEHRVIVNSEKERVSLAFFYNPKSDIPIQPMQQLVTST-MPPLYPPMTFDQY---RL 389

Query: 301 KNKTHPP---SRVEDEIS 308
             +T  P   S VE  IS
Sbjct: 390 FIRTQGPRGKSHVESHIS 398

BLAST of CmaCh01G007790 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 144.4 bits (363), Expect = 1.5e-34
Identity = 90/301 (29.90%), Postives = 158/301 (52.49%), Query Frame = 0

Query: 6   IPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSKTFFHY 65
           IP +D+S  LS+ +N+     I  +++AC  +GFFQ++NHGI +E +++  +++  FF  
Sbjct: 55  IPVIDLSK-LSKPDNDDFFFEILKLSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDM 114

Query: 66  PDEIKLQYSPKPGAPLLAGFNKQKTNCVDK-----NEYVL-VFPPGSKF-NIYPQEPPQF 125
           P E K +Y  +PG   + G+ +      D+     N + L V PP  +   ++P +P +F
Sbjct: 115 PLEEKKKYPMEPGT--VQGYGQAFIFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARF 174

Query: 126 KETLEEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVGE-- 185
            E+LE    ++ ++   +   + + LGL     ++   +       N  YYP     +  
Sbjct: 175 SESLEGYSKEIRELCKRLLKYIAISLGLKEERFEEMFGEAVQAVRMN--YYPPCSSPDLV 234

Query: 186 NGLIHHEDANCITLVIQ--DDAGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKKFK 245
            GL  H D + +T++ Q  +   GLQ+ KD+ W+PV P+  A+V+N+GD I+VLSN K+K
Sbjct: 235 LGLSPHSDGSALTVLQQSKNSCVGLQILKDNTWVPVKPLPNALVINIGDTIEVLSNGKYK 294

Query: 246 SATHRVVRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALRLKN 296
           S  HR V  + KER +   F + + +  +EP+ E   +     KY+ +   +Y    + N
Sbjct: 295 SVEHRAVTNREKERLTIVTFYAPNYEVEIEPMSELVDDETNPCKYRSYNHGDYSYHYVSN 350

BLAST of CmaCh01G007790 vs. TAIR 10
Match: AT3G11180.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 143.7 bits (361), Expect = 2.6e-34
Identity = 98/295 (33.22%), Postives = 147/295 (49.83%), Query Frame = 0

Query: 1   MANTGIPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSK 60
           +A+  IP +D+    S  E++ K+     I+EAC  +GFFQ++NHG+  E +  A +  K
Sbjct: 90  VADINIPIIDLDSLFSGNEDDKKR-----ISEACREWGFFQVINHGVKPELMDAARETWK 149

Query: 61  TFFHYPDEIKLQYSPKPGAPLLAG--FNKQKTNCVDKNE-YVLVFPPGS--KFNIYPQEP 120
           +FF+ P E K  YS  P      G     +K   +D N+ Y L F P +   FN +P  P
Sbjct: 150 SFFNLPVEAKEVYSNSPRTYEGYGSRLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLP 209

Query: 121 PQFKETLEEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVG 180
              +E  +E   +L  +   + +IL+  LGL    L++            + YYP     
Sbjct: 210 SNIREMNDEYGKELVKLGGRLMTILSSNLGLRAEQLQEAFGGEDVGACLRVNYYPKCPQP 269

Query: 181 E--NGLIHHEDANCITLVIQDD-AGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKK 240
           E   GL  H D   +T+++ DD   GLQV+    WI V P+  A +VN+GD IQ+LSN K
Sbjct: 270 ELALGLSPHSDPGGMTILLPDDQVVGLQVRHGDTWITVNPLRHAFIVNIGDQIQILSNSK 329

Query: 241 FKSATHRVVRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEY 288
           +KS  HRV+    KER S AFF +   D  ++P+ +        P Y    F++Y
Sbjct: 330 YKSVEHRVIVNSEKERVSLAFFYNPKSDIPIQPMQQLVTST-MPPLYPPMTFDQY 378

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZWQ95.1e-3530.07Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 ... [more]
Q9FFF66.6e-3534.60Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1[more]
O043958.6e-3533.22Flavonol synthase/flavanone 3-hydroxylase (Fragment) OS=Matthiola incana OX=3724... [more]
Q009851.1e-3431.141-aminocyclopropane-1-carboxylate oxidase 1 OS=Malus domestica OX=3750 PE=1 SV=1[more]
Q9SRM35.6e-3433.65Jasmonate-induced oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=JOX1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
O237634.4e-183100.00Gibberellin 7-oxidase OS=Cucurbita maxima OX=3661 GN=LOC111493375 PE=2 SV=1[more]
A0A6J1GB312.3e-17996.82flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A1S3B2M03.6e-15381.41flavonol synthase/flavanone 3-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
S0BE222.9e-15080.13GA 7-oxidase OS=Cucumis sativus OX=3659 GN=GA7ox1 PE=2 SV=1[more]
A0A5D3BJI54.9e-15081.91Flavonol synthase/flavanone 3-hydroxylase-like OS=Cucumis melo var. makuwa OX=11... [more]
Match NameE-valueIdentityDescription
XP_022998829.19.1e-183100.00flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita maxima] >AAB64346.1 gi... [more]
KAG6607440.13.6e-17997.13hypothetical protein SDJN03_00782, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022949101.14.7e-17996.82flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita moschata][more]
XP_023524991.18.0e-17996.82flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita pepo subsp. pepo][more]
XP_008441091.17.5e-15381.41PREDICTED: flavonol synthase/flavanone 3-hydroxylase-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT5G05600.14.7e-3634.602-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G19010.12.3e-3530.822-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G11180.14.0e-3533.652-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G21420.11.5e-3429.902-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G11180.22.6e-3433.222-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 1..298
e-value: 3.5E-81
score: 275.1
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 6..87
e-value: 2.1E-18
score: 67.2
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 165..255
e-value: 4.1E-22
score: 78.6
NoneNo IPR availablePANTHERPTHR10209:SF732FLAVONOL SYNTHASE/FLAVANONE 3-HYDROXYLASE-LIKEcoord: 1..313
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 1..313
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 4..295
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 159..257
score: 12.052887

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G007790.1CmaCh01G007790.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding