CmaCh01G007690 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh01G007690
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Pectate lyase
Location	Cma_Chr01: 4141420 .. 4143407 (+)
RNA-Seq Expression	CmaCh01G007690
Synteny	CmaCh01G007690

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCACTCAGCTCATTATCCTGTCGCTCATTTCCGTGGCACTCCTCGCGGCCGTTCCCCACACCGAAGCCCGCGCCATGAACATGAACGCCATCGACCGATGCTGGCGATCGAACCCATACTGGAGAAAAAACAGGCAAAAGCTCGCCCTTTGTTCAATTGGGTTTGTAGGAAAAATGACCAACAACATCGGAAGAAACCTCGTACACTACCAAGTCACGGACCCCACCGACGACCCACTAACCCCACGCCCAGGCACCCTCAGATACGGCGCCACAATGATCAAACCCAAAGTATGGATCACGTTTCAAAAAGACATGCACATTGTGCTTAACAAGCCGCTTCTTATTAGCAGCTACACGGCCATCGACGGCCGTGGCGCCACCGTCCACATCACGGGGAATGCGTGTCTGATGGTGTCTAGAGCCACCAATGTCATAATCCATGGGCTTATTATCCACCATTGCAAGGCCCAGGCGGCTGGCCGTGTAATGGGACCGGGTTCGAAGATCGTTTCATTGGGACACGTGGACGGTGATGCCATTCGTTTGGTTAGTGCTTCTAAGATTTGGATTGACCATAACACGCTGTATCGGTGTGAGGATGGGCTGATTGATGTCACTAGAGGCTCTACAGATATCACCATTTCTAACAATTGGTTGAGAGATCAAGACAAGGTTATACTTTTGGGTCACGACGATGATTATATTTGGGACCGAAACATGAAGGTTACTCTCGTTTATAACCATTTCGGACCTAATTGCAACCAAAGGATGCCCAGGTAACATTTAATATGGTATCAAACCATAACATAAATTTTAAGGCTAAATTAATAAAAATATTTGTAAATTTTGTATTTTATCATAAAAATATTCTTCAAAATTTTCAAAAATTTTAATAATGCTCTTAAACTCAAAAATACCGTTGACTTTAGAATAAATTGAAAACTATTAATAATTTTGTTTTAAAATACTTTAAAAAAAGTTTCAAAAATACCATTAAGCTTTTAAGAGTATTTTTAAAATACCTTCAAACTTTCTTAAATTTTATTGATATCAAGTTTTTTTTTTTAAGATATTAATTTTTTTAAAAAGTTAAAAAATAATTTGTATATGGATGAAATTGTTAGTACCTTTAAAAAATAACTAAAAATTTCTCAACTTCTATAATTTTTTTTTTCTTTTTTACCGTTCCCTTAAAATGGAAGATAGAGATGACATATGAGGTATAGATAGACATTTTCCGTTACAATAATAACTTTTTAATTTTATTTTACAAAAGTTCATGGATAATTATTAAACATAGTATTAATGGTAAGAATATTTTTCAGCGTTTTTTACTTCAAAAGTACAACAAAACTTTTGAAAGTTCAAAGGTATTTTGAAATAGATTATTAACCGTAACCATATTTTTCAAAGTTTAAAGGTAATATTGAATTCTTATAAATTTATAATTTTGAGACAAAATATAAAGTTTACGGATATTTTTTTATTTATTAATTTAACAAAATTTGGTATGAAAATACAGGATTAGATACGGGTATGCACACGTGGCGAACAATTTGTACCAAGGTTGGACACAATACGCAATTGGAGGCAGCATGAATCCTAGTGTGAAGAGTGAAGCCAACCTTTTCATGGCATCAAAATCAAAACAGGCAATAATAATAACAACAATATATCAAAACTCCATATTTGGTTAAAAAATTGAGCCTTTTCTTACCAAACAACAACAAATGCTTATTTGAAATCATAAAATGCAGATCATATGGAGCACAGGCAAAGTAGAGAACGCCAAGTGGAATTTCCATTCAGTAAGAGACGTTTTTGAAAACGGCGCTTCCTTCGCACAGATCGGCGCTGGCCGAGGCGGTGTCAAGCCACATTACAATGCCCTGCAGCGCTTCCCAGTGGTAGACGCCAAGTGGTTGCGGTCATTGACAAGCTCTTCCGGCGCCCTGCGCTGCTCACCACGCTCAAGGTGTTGA

mRNA sequence

ATGGCCACTCAGCTCATTATCCTGTCGCTCATTTCCGTGGCACTCCTCGCGGCCGTTCCCCACACCGAAGCCCGCGCCATGAACATGAACGCCATCGACCGATGCTGGCGATCGAACCCATACTGGAGAAAAAACAGGCAAAAGCTCGCCCTTTGTTCAATTGGGTTTGTAGGAAAAATGACCAACAACATCGGAAGAAACCTCGTACACTACCAAGTCACGGACCCCACCGACGACCCACTAACCCCACGCCCAGGCACCCTCAGATACGGCGCCACAATGATCAAACCCAAAGTATGGATCACGTTTCAAAAAGACATGCACATTGTGCTTAACAAGCCGCTTCTTATTAGCAGCTACACGGCCATCGACGGCCGTGGCGCCACCGTCCACATCACGGGGAATGCGTGTCTGATGGTGTCTAGAGCCACCAATGTCATAATCCATGGGCTTATTATCCACCATTGCAAGGCCCAGGCGGCTGGCCGTGTAATGGGACCGGGTTCGAAGATCGTTTCATTGGGACACGTGGACGGTGATGCCATTCGTTTGGTTAGTGCTTCTAAGATTTGGATTGACCATAACACGCTGTATCGGTGTGAGGATGGGCTGATTGATGTCACTAGAGGCTCTACAGATATCACCATTTCTAACAATTGGTTGAGAGATCAAGACAAGGTTATACTTTTGGGTCACGACGATGATTATATTTGGGACCGAAACATGAAGATCATATGGAGCACAGGCAAAGTAGAGAACGCCAAGTGGAATTTCCATTCAGTAAGAGACGTTTTTGAAAACGGCGCTTCCTTCGCACAGATCGGCGCTGGCCGAGGCGGTGTCAAGCCACATTACAATGCCCTGCAGCGCTTCCCAGTGGTAGACGCCAAGTGGTTGCGGTCATTGACAAGCTCTTCCGGCGCCCTGCGCTGCTCACCACGCTCAAGGTGTTGA

Coding sequence (CDS)

ATGGCCACTCAGCTCATTATCCTGTCGCTCATTTCCGTGGCACTCCTCGCGGCCGTTCCCCACACCGAAGCCCGCGCCATGAACATGAACGCCATCGACCGATGCTGGCGATCGAACCCATACTGGAGAAAAAACAGGCAAAAGCTCGCCCTTTGTTCAATTGGGTTTGTAGGAAAAATGACCAACAACATCGGAAGAAACCTCGTACACTACCAAGTCACGGACCCCACCGACGACCCACTAACCCCACGCCCAGGCACCCTCAGATACGGCGCCACAATGATCAAACCCAAAGTATGGATCACGTTTCAAAAAGACATGCACATTGTGCTTAACAAGCCGCTTCTTATTAGCAGCTACACGGCCATCGACGGCCGTGGCGCCACCGTCCACATCACGGGGAATGCGTGTCTGATGGTGTCTAGAGCCACCAATGTCATAATCCATGGGCTTATTATCCACCATTGCAAGGCCCAGGCGGCTGGCCGTGTAATGGGACCGGGTTCGAAGATCGTTTCATTGGGACACGTGGACGGTGATGCCATTCGTTTGGTTAGTGCTTCTAAGATTTGGATTGACCATAACACGCTGTATCGGTGTGAGGATGGGCTGATTGATGTCACTAGAGGCTCTACAGATATCACCATTTCTAACAATTGGTTGAGAGATCAAGACAAGGTTATACTTTTGGGTCACGACGATGATTATATTTGGGACCGAAACATGAAGATCATATGGAGCACAGGCAAAGTAGAGAACGCCAAGTGGAATTTCCATTCAGTAAGAGACGTTTTTGAAAACGGCGCTTCCTTCGCACAGATCGGCGCTGGCCGAGGCGGTGTCAAGCCACATTACAATGCCCTGCAGCGCTTCCCAGTGGTAGACGCCAAGTGGTTGCGGTCATTGACAAGCTCTTCCGGCGCCCTGCGCTGCTCACCACGCTCAAGGTGTTGA

Protein sequence

MATQLIILSLISVALLAAVPHTEARAMNMNAIDRCWRSNPYWRKNRQKLALCSIGFVGKMTNNIGRNLVHYQVTDPTDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDYIWDRNMKIIWSTGKVENAKWNFHSVRDVFENGASFAQIGAGRGGVKPHYNALQRFPVVDAKWLRSLTSSSGALRCSPRSRC

Homology

[Close]

BLAST of CmaCh01G007690 vs. ExPASy Swiss-Prot
Match: O65388 (Putative pectate lyase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g11920 PE=3 SV=2)

HSP 1 Score: 226.9 bits (577), Expect = 3.3e-58
Identity = 143/380 (37.63%), Postives = 193/380 (50.79%), Query Frame = 0


Query: 5   LIILSLISVALLAAVPHTEARAMN--------MNAIDRCWRSNPYWRKNRQKLALCSIGF 64
           L I+SL+  A  ++V   EA   N         N ID CWR NPYW  NR+ LA C++GF
Sbjct: 6   LTIISLLFAAFSSSV--VEAAYSNGYTIPKLLPNPIDSCWRRNPYWASNRRALADCAVGF 65

Query: 65  VGKMTNNIGRNLVHYQVTDPTDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKPLL 124
            GK     G+    Y VT+P+DDP  PRPGTLRY     KP +WITF +DM IVL   L+
Sbjct: 66  -GKSAVG-GKYGSIYVVTNPSDDPENPRPGTLRYAVIQSKP-LWITFARDMVIVLRNELI 125

Query: 125 ISSYTAIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGH 184
           ++SY  IDGRGA V I    C+ +   ++VIIHG+ IH CK   +GRV    + + S   
Sbjct: 126 MNSYKTIDGRGAKVEIAYGPCITIQHVSHVIIHGISIHDCKPGKSGRVRSSPTHVGSRKG 185

Query: 185 VDGDAIRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDY 244
            DGDAI +  +S IWIDH    RC+DGLIDV   ST +TISNN+    DKV+LLGH+D+ 
Sbjct: 186 SDGDAIAIFDSSHIWIDHCFFSRCQDGLIDVLHASTAVTISNNYFTQHDKVMLLGHNDNN 245

Query: 245 IWDRNMKI---------------------------------------------IWSTG-- 304
           + D+ M++                                             I+S G  
Sbjct: 246 VEDKIMRVTIAFNHFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGSADPTIFSEGNY 305

Query: 305 --------------KVENA----KWNFHSVRDVFENGASFAQIGAGRGGVKPHYNALQRF 312
                         ++++     +W + + +DVF+NGA F  + +G G V P Y   +RF
Sbjct: 306 FVASDDPSKKQVTKRIDSGYDWKRWKWRTSKDVFKNGAYF--VPSGYGTVTPLYGRAERF 365

[Close]

BLAST of CmaCh01G007690 vs. ExPASy Swiss-Prot
Match: Q9C8G4 (Probable pectate lyase 4 OS=Arabidopsis thaliana OX=3702 GN=At1g30350 PE=2 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 2.1e-57
Identity = 142/382 (37.17%), Postives = 190/382 (49.74%), Query Frame = 0


Query: 1   MATQLIILSLISVALLAAVPHT------EARAMNMNAIDRCWRSNPYWRKNRQKLALCSI 60
           MA+ ++I+SL+  A  + +  T       A  +++N ID CWR NP W  NRQ LA C++
Sbjct: 1   MASLVVIVSLLLAAFASPLLETAHSYNVTAPRVSLNPIDACWRRNPKWATNRQALAHCAV 60

Query: 61  GFVGKMTNNIGRNLVHYQVTDPTDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKP 120
           G+ GK     G+N   Y VT+P+D+P  P PGTLRY  +  KP +WITF +DM IVL   
Sbjct: 61  GY-GKAAIG-GKNGPIYVVTNPSDNPTRPSPGTLRYAVSQPKP-LWITFARDMVIVLKSQ 120

Query: 121 LLISSYTAIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSL 180
           L+I+SY  IDGRGA V I    CL + +  +VIIHG+ IH CKA   G            
Sbjct: 121 LMINSYKTIDGRGAKVEIANGPCLRIRQVKHVIIHGISIHDCKADPNG------------ 180

Query: 181 GHVDGDAIRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDD 240
             +DGD IR+  ++ +WIDH  L RC DGLIDV   ST +TISNN+    DKV+LLGHDD
Sbjct: 181 --MDGDGIRVFQSTHVWIDHCFLSRCHDGLIDVIVSSTAVTISNNYFTQHDKVMLLGHDD 240

Query: 241 DYIWDRNMK---------------------------------------------IIWSTG 300
            Y+ D++M+                                             II+S G
Sbjct: 241 SYMGDKDMRVTIAFNTFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGSANPIIFSEG 300

Query: 301 KV--------------------ENAKWNFHSVRDVFENGASFAQIGAGRGGVKPHYNALQ 312
                                 ++ +W + + RDVF NGA F   G     V+P Y   +
Sbjct: 301 NYFVAPEKRSSKQVTKRMMAGPDSKRWKWGTSRDVFMNGAFFGPPGV---IVRPLYKGGE 360

[Close]

BLAST of CmaCh01G007690 vs. ExPASy Swiss-Prot
Match: Q9LTZ0 (Putative pectate lyase 11 OS=Arabidopsis thaliana OX=3702 GN=At3g27400 PE=3 SV=2)

HSP 1 Score: 216.9 bits (551), Expect = 3.4e-55
Identity = 122/286 (42.66%), Postives = 161/286 (56.29%), Query Frame = 0


Query: 30  NAIDRCWRSNPYWRKNRQKLALCSIGFVGKMTNNIGRNLVHYQVTDP-TDDPLTPRPGTL 89
           N ID CWR +P W  NRQ+LA C+IGF GK     GR    Y VTDP  DDP+ PRPGTL
Sbjct: 64  NPIDDCWRCDPNWETNRQRLADCAIGF-GKNAIG-GRKGRIYVVTDPANDDPVNPRPGTL 123

Query: 90  RYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDGRGATVHITGNACLMVSRATNVII 149
           RY  T  +P +WI F++DM I L K L+I+S+  IDGRG++VHIT   CL +  ATN+II
Sbjct: 124 RYAVTQEEP-LWIIFKRDMVIRLKKELIITSFKTIDGRGSSVHITDGPCLKIHYATNIII 183

Query: 150 HGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLVSASKIWIDHNTLYRCEDGLIDVT 209
           HG+ IH CK  + G +             DGDA+ +     +WIDH +L  C+DGLID  
Sbjct: 184 HGINIHDCKPGSGGMIKDGPHHTGWWMQSDGDAVAIFGGKHVWIDHCSLSNCDDGLIDAI 243

Query: 210 RGSTDITISNNWLRDQDKVILLGHDDDYIWDRNMKII-----WSTGKVENAKWNFHSVRD 269
            GST ITISNN +   DKV+LLGH D Y  D+NM++      +  G V+      H    
Sbjct: 244 HGSTAITISNNHMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFH 303

Query: 270 VFENGASFAQIGAGRGGVKPH-YNALQRFPVVDAKWLRSLTSSSGA 309
           V  N  +  ++ A  G   P  Y+   RF   + ++ + +T    A
Sbjct: 304 VVNNDYTHWEMYAIGGSASPTIYSQGNRFLAPNTRFNKEVTKHEDA 346

[Close]

BLAST of CmaCh01G007690 vs. ExPASy Swiss-Prot
Match: O65456 (Probable pectate lyase 16 OS=Arabidopsis thaliana OX=3702 GN=At4g22080 PE=2 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 4.4e-55
Identity = 129/353 (36.54%), Postives = 169/353 (47.88%), Query Frame = 0


Query: 29  MNAIDRCWRSNPYWRKNRQKLALCSIGFVGKMTNNIGRNLVHYQVTDPTDDPLTPRPGTL 88
           MN +D CWR    W  NR+ LA C +GF          NL  Y VT+P D+   P+PG+L
Sbjct: 46  MNPVDSCWRLKSDWAANRKDLADCVVGFGSSTLGGKKGNL--YVVTNPYDNAQNPQPGSL 105

Query: 89  RYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDGRGATVHITGNACLMVSRATNVII 148
           RYG    KP +WITF KDM I L   L+++SY  IDGRGA V I    C+ +   TNVI+
Sbjct: 106 RYGVIQAKP-LWITFAKDMVITLENELMVNSYKTIDGRGAKVEIAYGPCITIQDVTNVIV 165

Query: 149 HGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLVSASKIWIDHNTLYRCEDGLIDVT 208
           HG+ IH CK    G V    + +      DGDAI +  +S IWIDH  L  C DGLIDV 
Sbjct: 166 HGISIHDCKPGKYGMVRSSPTHVGHRKGSDGDAIAIFGSSNIWIDHCYLASCTDGLIDVI 225

Query: 209 RGSTDITISNNWLRDQDKVILLGHDDDYIWDRNMKI------------------------ 268
             ST ITISNN+    DKV+LLGH+DD++ D  MK+                        
Sbjct: 226 HASTGITISNNYFTQHDKVMLLGHNDDFVQDVKMKVTVAFNHFGPGLVERMPRVRRGYAH 285

Query: 269 ---------------------IWSTGKV------ENAK-------------WNFHSVRDV 318
                                I+S G         N+K             W + + +DV
Sbjct: 286 VANNRYDKWIMYAIGGSADPTIFSEGNYFIASDKSNSKEVTKREVKGGWNNWRWRTSKDV 345

[Close]

BLAST of CmaCh01G007690 vs. ExPASy Swiss-Prot
Match: O65457 (Putative pectate lyase 17 OS=Arabidopsis thaliana OX=3702 GN=At4g22090 PE=3 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.3e-54
Identity = 129/366 (35.25%), Postives = 173/366 (47.27%), Query Frame = 0


Query: 17  AAVPHTEARAMN-MNAIDRCWRSNPYWRKNRQKLALCSIGFVGKMTNNIGRNLVHYQVTD 76
           AA P+      N MN +D CWR    W  NR+ LA C++GF          N+  Y VT+
Sbjct: 33  AAAPYPAEEPQNIMNPVDSCWRLKSDWDVNREDLADCAVGFGSSTLGGKKGNI--YVVTN 92

Query: 77  PTDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDGRGATVHITGN 136
           P D+   P PG+LRYG    KP +WITF KDM I L   L+++SY  IDGRGA V I   
Sbjct: 93  PYDNAQNPHPGSLRYGVIQAKP-LWITFAKDMVITLANELMVNSYKTIDGRGAKVEIAYG 152

Query: 137 ACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLVSASKIWIDHN 196
            C+ +   TNVI+HG+ IH CK   +G+V    + +      DGDAI +  +S +WIDH 
Sbjct: 153 PCITIQDVTNVIVHGISIHDCKPGKSGKVRSSPTHVGHRKGSDGDAITIFGSSNVWIDHC 212

Query: 197 TLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDYIWDRNMKI----------- 256
            L  C DGLIDV   ST ITISNN+    DKV+LLGH+D+++ D  MK+           
Sbjct: 213 YLASCTDGLIDVIHASTAITISNNYFTQHDKVMLLGHNDNFVKDVKMKVTVAFNHFGPGL 272

Query: 257 ----------------------------------IWSTGKVENA---------------- 316
                                             I+S G    A                
Sbjct: 273 VERMPRVRRGYAHVANNRYDKWIMYAIGGSADPTIFSEGNYFIASDKSYSKEVTKREVKG 332

Query: 317 ---KWNFHSVRDVFENGASFAQIGAGRGGVKPHYNALQRFPVVDAKWLRSLTSSSGALRC 318
               W + +  DVF+NGA F  + +G G +   Y++ QRF V     + SLT+ +G L C
Sbjct: 333 GWNNWRWRTSNDVFKNGAFF--VPSGYGSIPLPYSSAQRFTVAPGNLVPSLTADAGPLNC 392

[Close]

BLAST of CmaCh01G007690 vs. ExPASy TrEMBL
Match: A0A6J1KAY1 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111492658 PE=3 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 1.0e-163
Identity = 291/352 (82.67%), Postives = 291/352 (82.67%), Query Frame = 0


Query: 27  MNMNAIDRCWRSNPYWRKNRQKLALCSIGFVGKMTNNIGRNLVHYQVTDPTDDPLTPRPG 86
           MNMNAIDRCWRSNPYWRKNRQKLALCSIGFVGKMTNNIGRNLVHYQVTDPTDDPLTPRPG
Sbjct: 1   MNMNAIDRCWRSNPYWRKNRQKLALCSIGFVGKMTNNIGRNLVHYQVTDPTDDPLTPRPG 60

Query: 87  TLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDGRGATVHITGNACLMVSRATNV 146
           TLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDGRGATVHITGNACLMVSRATNV
Sbjct: 61  TLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDGRGATVHITGNACLMVSRATNV 120

Query: 147 IIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLVSASKIWIDHNTLYRCEDGLID 206
           IIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLVSASKIWIDHNTLYRCEDGLID
Sbjct: 121 IIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLVSASKIWIDHNTLYRCEDGLID 180

Query: 207 VTRGSTDITISNNWLRDQDKVILLGHDDDYIWDRNMK----------------------- 266
           VTRGSTDITISNNWLRDQDKVILLGHDDDYIWDRNMK                       
Sbjct: 181 VTRGSTDITISNNWLRDQDKVILLGHDDDYIWDRNMKVTLVYNHFGPNCNQRMPRIRYGY 240

Query: 267 --------------------------------------IIWSTGKVENAKWNFHSVRDVF 318
                                                 IIWSTGKVENAKWNFHSVRDVF
Sbjct: 241 AHVANNLYQGWTQYAIGGSMNPSVKSEANLFMASKSKQIIWSTGKVENAKWNFHSVRDVF 300

[Close]

BLAST of CmaCh01G007690 vs. ExPASy TrEMBL
Match: A0A6J1E7R9 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111431420 PE=3 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 2.7e-148
Identity = 260/377 (68.97%), Postives = 293/377 (77.72%), Query Frame = 0


Query: 2   ATQLIILSLISVALLAAVPHTEARAMNMNAIDRCWRSNPYWRKNRQKLALCSIGFVGKMT 61
           ATQL +LSLIS+ LL+AV ++E   + MNAIDRCWRSNPYWR+NRQ+L +CS+GFVGKMT
Sbjct: 3   ATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMT 62

Query: 62  NNIGRNLVHYQVTDPTDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYT 121
           NNIGRNL+ YQVTDP+DDP+ PRPGTLRYGATMIK KVWITFQKDMHI+L+KPLLISSYT
Sbjct: 63  NNIGRNLIRYQVTDPSDDPINPRPGTLRYGATMIKSKVWITFQKDMHILLHKPLLISSYT 122

Query: 122 AIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDA 181
           AIDGRGA+VHI+GNACL+VS+ATN+IIHGLIIHHCKAQ AG+VMGP SK+VSLGHVDGDA
Sbjct: 123 AIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDA 182

Query: 182 IRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDYIWDRN 241
           IRLVSASK+WIDHNTLYRCEDGL+DVTRGSTDITISNNW RDQDKVILLGH+D Y+ D+N
Sbjct: 183 IRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRDKN 242

Query: 242 MK---------------------------------------------------------- 301
           M+                                                          
Sbjct: 243 MRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIASK 302

Query: 302 ---IIWSTGKVENAKWNFHSVRDVFENGASFAQIGAGRGGVKPHYNALQRFPVVDAKWLR 318
              I+WSTGKVE AKW FHSVRDVFENGASF QIG GRGGVKP+Y+A QRFPV DAKW+R
Sbjct: 303 SKQIVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKWVR 362

[Close]

BLAST of CmaCh01G007690 vs. ExPASy TrEMBL
Match: A0A6J1KLU6 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111495358 PE=3 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 1.5e-146
Identity = 258/377 (68.44%), Postives = 291/377 (77.19%), Query Frame = 0


Query: 2   ATQLIILSLISVALLAAVPHTEARAMNMNAIDRCWRSNPYWRKNRQKLALCSIGFVGKMT 61
           ATQL +LSLIS+ LL+AV ++E   + MNAIDRCWRSNPYWR+NRQ+L +CS+GFVGKMT
Sbjct: 3   ATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMT 62

Query: 62  NNIGRNLVHYQVTDPTDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYT 121
           NNIGRNL+ YQVTDP+DDP+ PRPGTLRYGATMIK KVWITFQK MHI+L+KPLLISSYT
Sbjct: 63  NNIGRNLIRYQVTDPSDDPINPRPGTLRYGATMIKSKVWITFQKGMHILLHKPLLISSYT 122

Query: 122 AIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDA 181
           AIDGRGA+VHI+GNACL+VS+ATN+IIHGLIIHHCKAQ AG+VMGP SK+VSLGHVDGDA
Sbjct: 123 AIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDA 182

Query: 182 IRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDYIWDRN 241
           IRLVSASK+WIDHNTLYRCEDGL+DVTRGSTDITISNNW RDQDKVILLGH+D Y+ D+N
Sbjct: 183 IRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRDKN 242

Query: 242 MK---------------------------------------------------------- 301
           M+                                                          
Sbjct: 243 MRVTVIYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIASK 302

Query: 302 ---IIWSTGKVENAKWNFHSVRDVFENGASFAQIGAGRGGVKPHYNALQRFPVVDAKWLR 318
              I+WSTGKVE AKW FHSV DVFENGASF QIG GRGGVKP+Y+A QRFPV DAKW+R
Sbjct: 303 LKQIVWSTGKVEAAKWKFHSVGDVFENGASFEQIGVGRGGVKPNYSAQQRFPVADAKWVR 362

[Close]

BLAST of CmaCh01G007690 vs. ExPASy TrEMBL
Match: A0A5D3BIF3 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00620 PE=3 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 6.9e-144
Identity = 253/365 (69.32%), Postives = 282/365 (77.26%), Query Frame = 0


Query: 4   QLIILSLISVALLAA--VPHTEARAMNMNAIDRCWRSNPYWRKNRQKLALCSIGFVGKMT 63
           QL +LSLIS   L +  V  +EA  ++MNAIDRCWRSNPYWR+NRQ+LA CS+GF GKMT
Sbjct: 6   QLSVLSLISFIFLISNVVSSSEAHGLSMNAIDRCWRSNPYWRRNRQQLATCSVGFAGKMT 65

Query: 64  NNIGRNLVHYQVTDPTDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYT 123
           NN+GRNL+HYQVTDP+DDP+ PRPGTLRYGATMIK KVWITFQKDMHIVL KPLLISS+T
Sbjct: 66  NNMGRNLIHYQVTDPSDDPINPRPGTLRYGATMIKHKVWITFQKDMHIVLQKPLLISSFT 125

Query: 124 AIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDA 183
           AIDGRGATVHITGNACLMV RATN+IIHGLIIHHC++QAAG VMGP SK++SLGHVDGDA
Sbjct: 126 AIDGRGATVHITGNACLMVLRATNIIIHGLIIHHCRSQAAGEVMGPNSKVISLGHVDGDA 185

Query: 184 IRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDYIWDRN 243
           IRLV+ASK+WIDHNTLYRCEDGL+DVTRGSTDITISNNW RDQDKVILLGHDD Y+ DRN
Sbjct: 186 IRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDDGYVRDRN 245

Query: 244 MK-------------------------------------------------IIWSTGKVE 303
           MK                                                 +IWSTG VE
Sbjct: 246 MKVNHMCRIRYGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIASKSKQVIWSTGNVE 305

Query: 304 NAKWNFHSVRDVFENGASFAQIGAGRGGVKPHYNALQRFPVVDAKWLRSLTSSSGALRCS 318
            AKW FHSVRDVFENGA F +IG GRG VKP+YN  QRFPV DAKW+R+LTSSSGALRC+
Sbjct: 306 EAKWKFHSVRDVFENGALFTEIGVGRGRVKPNYNPQQRFPVADAKWIRALTSSSGALRCT 365

[Close]

BLAST of CmaCh01G007690 vs. ExPASy TrEMBL
Match: A0A5A7T3T4 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold112G00520 PE=3 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 2.0e-143
Identity = 253/365 (69.32%), Postives = 281/365 (76.99%), Query Frame = 0


Query: 4   QLIILSLISVALLAA--VPHTEARAMNMNAIDRCWRSNPYWRKNRQKLALCSIGFVGKMT 63
           QL  LSLIS   L +  V  +EA  ++MNAIDRCWRSNPYWR+NRQ+LA CS+GF GKMT
Sbjct: 6   QLSALSLISFIFLISNVVSSSEAHGLSMNAIDRCWRSNPYWRRNRQQLATCSVGFAGKMT 65

Query: 64  NNIGRNLVHYQVTDPTDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYT 123
           NN+GRNL+HYQVTDP+DDP+ PRPGTLRYGATMIK KVWITFQKDMHIVL KPLLISS+T
Sbjct: 66  NNMGRNLIHYQVTDPSDDPINPRPGTLRYGATMIKHKVWITFQKDMHIVLQKPLLISSFT 125

Query: 124 AIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDA 183
           AIDGRGATVHITGNACLMV RATN+IIHGLIIHHC++QAAG VMGP SK++SLGHVDGDA
Sbjct: 126 AIDGRGATVHITGNACLMVLRATNIIIHGLIIHHCRSQAAGEVMGPNSKVISLGHVDGDA 185

Query: 184 IRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDYIWDRN 243
           IRLV+ASK+WIDHNTLYRCEDGL+DVTRGSTDITISNNW RDQDKVILLGHDD Y+ DRN
Sbjct: 186 IRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDDGYVRDRN 245

Query: 244 MK-------------------------------------------------IIWSTGKVE 303
           MK                                                 +IWSTG VE
Sbjct: 246 MKVNHMCRIRYGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIASKSKQVIWSTGNVE 305

Query: 304 NAKWNFHSVRDVFENGASFAQIGAGRGGVKPHYNALQRFPVVDAKWLRSLTSSSGALRCS 318
            AKW FHSVRDVFENGA F +IG GRG VKP+YN  QRFPV DAKW+R+LTSSSGALRC+
Sbjct: 306 EAKWKFHSVRDVFENGALFTEIGVGRGRVKPNYNPQQRFPVADAKWIRALTSSSGALRCT 365

[Close]

BLAST of CmaCh01G007690 vs. NCBI nr
Match: KAG7037086.1 (putative pectate lyase 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 630.9 bits (1626), Expect = 5.8e-177
Identity = 307/317 (96.85%), Postives = 311/317 (98.11%), Query Frame = 0


Query: 1   MATQLIILSLISVALLAAVPHTEARAMNMNAIDRCWRSNPYWRKNRQKLALCSIGFVGKM 60
           MATQLIILSLIS++LLAAV HTEARAMNMNAIDRCWR NPYWRKNRQKLALCSIGFVGKM
Sbjct: 36  MATQLIILSLISLSLLAAVSHTEARAMNMNAIDRCWRPNPYWRKNRQKLALCSIGFVGKM 95

Query: 61  TNNIGRNLVHYQVTDPTDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKPLLISSY 120
           TNNIGRNLVHYQVTDP+DDPLTPR GTLRYGATMIKPKVWITFQKDMHIVLNKPLLISSY
Sbjct: 96  TNNIGRNLVHYQVTDPSDDPLTPRSGTLRYGATMIKPKVWITFQKDMHIVLNKPLLISSY 155

Query: 121 TAIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGD 180
           TAIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGD
Sbjct: 156 TAIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGD 215

Query: 181 AIRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDYIWDR 240
           AIRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDY  DR
Sbjct: 216 AIRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDYFRDR 275

Query: 241 NMKIIWSTGKVENAKWNFHSVRDVFENGASFAQIGAGRGGVKPHYNALQRFPVVDAKWLR 300
           NMKIIWSTGKVENAKW FHSVRDVFENGASFAQIGAGRGGVKP+YNALQRFPVVDAKWLR
Sbjct: 276 NMKIIWSTGKVENAKWKFHSVRDVFENGASFAQIGAGRGGVKPNYNALQRFPVVDAKWLR 335

Query: 301 SLTSSSGALRCSPRSRC 318
           SLTSSSGALRCSPRSRC
Sbjct: 336 SLTSSSGALRCSPRSRC 352

[Close]

BLAST of CmaCh01G007690 vs. NCBI nr
Match: KAG6607417.1 (putative pectate lyase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 603.6 bits (1555), Expect = 9.8e-169
Identity = 302/344 (87.79%), Postives = 307/344 (89.24%), Query Frame = 0


Query: 1   MATQLIILSLISVALLAAVPHTEARAMNMNAIDRCWRSNPYWRKNRQKLALCSIGFVGKM 60
           MATQLIILSLIS++LLAAV HTEARAMNMNAIDRCWR NPYWRKNRQKLALCSIGFVGKM
Sbjct: 1   MATQLIILSLISLSLLAAVSHTEARAMNMNAIDRCWRPNPYWRKNRQKLALCSIGFVGKM 60

Query: 61  TNNIGRNLVHYQVTDPTDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKPLLISSY 120
           TNNIGRNLVHYQVTDP+DDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKPLLISSY
Sbjct: 61  TNNIGRNLVHYQVTDPSDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKPLLISSY 120

Query: 121 TAIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGD 180
           TAIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGD
Sbjct: 121 TAIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGD 180

Query: 181 AIRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDYIWDR 240
           AIRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDY  DR
Sbjct: 181 AIRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDYFRDR 240

Query: 241 NMKII---------------------------WSTGKVENAKWNFHSVRDVFENGASFAQ 300
           NMK+                               GKVENAKW FHSVRDVFENGASFAQ
Sbjct: 241 NMKVTLVYNHFGPNCNQRMPRIRYGYAHVANNLYQGKVENAKWKFHSVRDVFENGASFAQ 300

Query: 301 IGAGRGGVKPHYNALQRFPVVDAKWLRSLTSSSGALRCSPRSRC 318
           IGAGRGGVKP+YNALQRF VVDAKWLRSLTSSSGALRCSPRSRC
Sbjct: 301 IGAGRGGVKPNYNALQRFLVVDAKWLRSLTSSSGALRCSPRSRC 344

[Close]

BLAST of CmaCh01G007690 vs. NCBI nr
Match: XP_022997815.1 (putative pectate lyase 2 isoform X2 [Cucurbita maxima])

HSP 1 Score: 585.9 bits (1509), Expect = 2.1e-163
Identity = 291/352 (82.67%), Postives = 291/352 (82.67%), Query Frame = 0


Query: 27  MNMNAIDRCWRSNPYWRKNRQKLALCSIGFVGKMTNNIGRNLVHYQVTDPTDDPLTPRPG 86
           MNMNAIDRCWRSNPYWRKNRQKLALCSIGFVGKMTNNIGRNLVHYQVTDPTDDPLTPRPG
Sbjct: 1   MNMNAIDRCWRSNPYWRKNRQKLALCSIGFVGKMTNNIGRNLVHYQVTDPTDDPLTPRPG 60

Query: 87  TLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDGRGATVHITGNACLMVSRATNV 146
           TLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDGRGATVHITGNACLMVSRATNV
Sbjct: 61  TLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDGRGATVHITGNACLMVSRATNV 120

Query: 147 IIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLVSASKIWIDHNTLYRCEDGLID 206
           IIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLVSASKIWIDHNTLYRCEDGLID
Sbjct: 121 IIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLVSASKIWIDHNTLYRCEDGLID 180

Query: 207 VTRGSTDITISNNWLRDQDKVILLGHDDDYIWDRNMK----------------------- 266
           VTRGSTDITISNNWLRDQDKVILLGHDDDYIWDRNMK                       
Sbjct: 181 VTRGSTDITISNNWLRDQDKVILLGHDDDYIWDRNMKVTLVYNHFGPNCNQRMPRIRYGY 240

Query: 267 --------------------------------------IIWSTGKVENAKWNFHSVRDVF 318
                                                 IIWSTGKVENAKWNFHSVRDVF
Sbjct: 241 AHVANNLYQGWTQYAIGGSMNPSVKSEANLFMASKSKQIIWSTGKVENAKWNFHSVRDVF 300

[Close]

BLAST of CmaCh01G007690 vs. NCBI nr
Match: XP_022923821.1 (putative pectate lyase 2 [Cucurbita moschata])

HSP 1 Score: 534.6 bits (1376), Expect = 5.6e-148
Identity = 260/377 (68.97%), Postives = 293/377 (77.72%), Query Frame = 0


Query: 2   ATQLIILSLISVALLAAVPHTEARAMNMNAIDRCWRSNPYWRKNRQKLALCSIGFVGKMT 61
           ATQL +LSLIS+ LL+AV ++E   + MNAIDRCWRSNPYWR+NRQ+L +CS+GFVGKMT
Sbjct: 3   ATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMT 62

Query: 62  NNIGRNLVHYQVTDPTDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYT 121
           NNIGRNL+ YQVTDP+DDP+ PRPGTLRYGATMIK KVWITFQKDMHI+L+KPLLISSYT
Sbjct: 63  NNIGRNLIRYQVTDPSDDPINPRPGTLRYGATMIKSKVWITFQKDMHILLHKPLLISSYT 122

Query: 122 AIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDA 181
           AIDGRGA+VHI+GNACL+VS+ATN+IIHGLIIHHCKAQ AG+VMGP SK+VSLGHVDGDA
Sbjct: 123 AIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDA 182

Query: 182 IRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDYIWDRN 241
           IRLVSASK+WIDHNTLYRCEDGL+DVTRGSTDITISNNW RDQDKVILLGH+D Y+ D+N
Sbjct: 183 IRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRDKN 242

Query: 242 MK---------------------------------------------------------- 301
           M+                                                          
Sbjct: 243 MRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIASK 302

Query: 302 ---IIWSTGKVENAKWNFHSVRDVFENGASFAQIGAGRGGVKPHYNALQRFPVVDAKWLR 318
              I+WSTGKVE AKW FHSVRDVFENGASF QIG GRGGVKP+Y+A QRFPV DAKW+R
Sbjct: 303 SKQIVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKWVR 362

[Close]

BLAST of CmaCh01G007690 vs. NCBI nr
Match: KAG7020030.1 (putative pectate lyase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 534.3 bits (1375), Expect = 7.3e-148
Identity = 259/377 (68.70%), Postives = 293/377 (77.72%), Query Frame = 0


Query: 2   ATQLIILSLISVALLAAVPHTEARAMNMNAIDRCWRSNPYWRKNRQKLALCSIGFVGKMT 61
           ATQL +LSLIS+ LL+AV ++E   + MNAIDRCWRSNPYWR+NRQ+L +CS+GFVGKMT
Sbjct: 3   ATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMT 62

Query: 62  NNIGRNLVHYQVTDPTDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYT 121
           NN+GRNL+ YQVTDP+DDP+ PRPGTLRYGATMIK KVWITFQKDMHI+L+KPLLISSYT
Sbjct: 63  NNVGRNLIRYQVTDPSDDPINPRPGTLRYGATMIKSKVWITFQKDMHILLHKPLLISSYT 122

Query: 122 AIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDA 181
           AIDGRGA+VHI+GNACL+VS+ATN+IIHGLIIHHCKAQ AG+VMGP SK+VSLGHVDGDA
Sbjct: 123 AIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDA 182

Query: 182 IRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDYIWDRN 241
           IRLVSASK+WIDHNTLYRCEDGL+DVTRGSTDITISNNW RDQDKVILLGH+D Y+ D+N
Sbjct: 183 IRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRDKN 242

Query: 242 MK---------------------------------------------------------- 301
           M+                                                          
Sbjct: 243 MRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIASK 302

Query: 302 ---IIWSTGKVENAKWNFHSVRDVFENGASFAQIGAGRGGVKPHYNALQRFPVVDAKWLR 318
              I+WSTGKVE AKW FHSVRDVFENGASF QIG GRGGVKP+Y+A QRFPV DAKW+R
Sbjct: 303 SKQIVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKWVR 362

[Close]

BLAST of CmaCh01G007690 vs. TAIR 10
Match: AT5G09280.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 286.2 bits (731), Expect = 3.2e-77
Identity = 128/185 (69.19%), Postives = 159/185 (85.95%), Query Frame = 0


Query: 60  MTNNIGRNLVHYQVTDPTDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKPLLISS 119
           MT NIG+ +  Y+VTDP+DDPL P+PGTLRYGAT++K K WITF+++M I L+KPLLISS
Sbjct: 1   MTGNIGKGVTQYKVTDPSDDPLNPKPGTLRYGATLVKGKKWITFKRNMKIKLHKPLLISS 60

Query: 120 YTAIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDG 179
           +TA+DGRGA+VHI+G ACL+V RAT+VIIHGL IH CKA     VMGP SKI+ LGH+DG
Sbjct: 61  FTALDGRGASVHISGPACLIVYRATDVIIHGLKIHDCKAHPPSSVMGPDSKIIQLGHMDG 120

Query: 180 DAIRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDYIWD 239
           DAIRLV+A K+WIDHNTLY CEDGL+DVT GSTD+T+SNNW R+QDKV+LLGHDD Y+ D
Sbjct: 121 DAIRLVTAKKVWIDHNTLYDCEDGLLDVTLGSTDVTVSNNWFRNQDKVMLLGHDDGYVKD 180

Query: 240 RNMKI 245
           ++M++
Sbjct: 181 KDMRV 185

[Close]

BLAST of CmaCh01G007690 vs. TAIR 10
Match: AT1G11920.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 226.9 bits (577), Expect = 2.3e-59
Identity = 143/380 (37.63%), Postives = 193/380 (50.79%), Query Frame = 0


Query: 5   LIILSLISVALLAAVPHTEARAMN--------MNAIDRCWRSNPYWRKNRQKLALCSIGF 64
           L I+SL+  A  ++V   EA   N         N ID CWR NPYW  NR+ LA C++GF
Sbjct: 6   LTIISLLFAAFSSSV--VEAAYSNGYTIPKLLPNPIDSCWRRNPYWASNRRALADCAVGF 65

Query: 65  VGKMTNNIGRNLVHYQVTDPTDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKPLL 124
            GK     G+    Y VT+P+DDP  PRPGTLRY     KP +WITF +DM IVL   L+
Sbjct: 66  -GKSAVG-GKYGSIYVVTNPSDDPENPRPGTLRYAVIQSKP-LWITFARDMVIVLRNELI 125

Query: 125 ISSYTAIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSLGH 184
           ++SY  IDGRGA V I    C+ +   ++VIIHG+ IH CK   +GRV    + + S   
Sbjct: 126 MNSYKTIDGRGAKVEIAYGPCITIQHVSHVIIHGISIHDCKPGKSGRVRSSPTHVGSRKG 185

Query: 185 VDGDAIRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDDDY 244
            DGDAI +  +S IWIDH    RC+DGLIDV   ST +TISNN+    DKV+LLGH+D+ 
Sbjct: 186 SDGDAIAIFDSSHIWIDHCFFSRCQDGLIDVLHASTAVTISNNYFTQHDKVMLLGHNDNN 245

Query: 245 IWDRNMKI---------------------------------------------IWSTG-- 304
           + D+ M++                                             I+S G  
Sbjct: 246 VEDKIMRVTIAFNHFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGSADPTIFSEGNY 305

Query: 305 --------------KVENA----KWNFHSVRDVFENGASFAQIGAGRGGVKPHYNALQRF 312
                         ++++     +W + + +DVF+NGA F  + +G G V P Y   +RF
Sbjct: 306 FVASDDPSKKQVTKRIDSGYDWKRWKWRTSKDVFKNGAYF--VPSGYGTVTPLYGRAERF 365

[Close]

BLAST of CmaCh01G007690 vs. TAIR 10
Match: AT1G30350.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 224.2 bits (570), Expect = 1.5e-58
Identity = 142/382 (37.17%), Postives = 190/382 (49.74%), Query Frame = 0


Query: 1   MATQLIILSLISVALLAAVPHT------EARAMNMNAIDRCWRSNPYWRKNRQKLALCSI 60
           MA+ ++I+SL+  A  + +  T       A  +++N ID CWR NP W  NRQ LA C++
Sbjct: 1   MASLVVIVSLLLAAFASPLLETAHSYNVTAPRVSLNPIDACWRRNPKWATNRQALAHCAV 60

Query: 61  GFVGKMTNNIGRNLVHYQVTDPTDDPLTPRPGTLRYGATMIKPKVWITFQKDMHIVLNKP 120
           G+ GK     G+N   Y VT+P+D+P  P PGTLRY  +  KP +WITF +DM IVL   
Sbjct: 61  GY-GKAAIG-GKNGPIYVVTNPSDNPTRPSPGTLRYAVSQPKP-LWITFARDMVIVLKSQ 120

Query: 121 LLISSYTAIDGRGATVHITGNACLMVSRATNVIIHGLIIHHCKAQAAGRVMGPGSKIVSL 180
           L+I+SY  IDGRGA V I    CL + +  +VIIHG+ IH CKA   G            
Sbjct: 121 LMINSYKTIDGRGAKVEIANGPCLRIRQVKHVIIHGISIHDCKADPNG------------ 180

Query: 181 GHVDGDAIRLVSASKIWIDHNTLYRCEDGLIDVTRGSTDITISNNWLRDQDKVILLGHDD 240
             +DGD IR+  ++ +WIDH  L RC DGLIDV   ST +TISNN+    DKV+LLGHDD
Sbjct: 181 --MDGDGIRVFQSTHVWIDHCFLSRCHDGLIDVIVSSTAVTISNNYFTQHDKVMLLGHDD 240

Query: 241 DYIWDRNMK---------------------------------------------IIWSTG 300
            Y+ D++M+                                             II+S G
Sbjct: 241 SYMGDKDMRVTIAFNTFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGSANPIIFSEG 300

Query: 301 KV--------------------ENAKWNFHSVRDVFENGASFAQIGAGRGGVKPHYNALQ 312
                                 ++ +W + + RDVF NGA F   G     V+P Y   +
Sbjct: 301 NYFVAPEKRSSKQVTKRMMAGPDSKRWKWGTSRDVFMNGAFFGPPGV---IVRPLYKGGE 360

[Close]

BLAST of CmaCh01G007690 vs. TAIR 10
Match: AT3G27400.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 216.9 bits (551), Expect = 2.4e-56
Identity = 122/286 (42.66%), Postives = 161/286 (56.29%), Query Frame = 0


Query: 30  NAIDRCWRSNPYWRKNRQKLALCSIGFVGKMTNNIGRNLVHYQVTDP-TDDPLTPRPGTL 89
           N ID CWR +P W  NRQ+LA C+IGF GK     GR    Y VTDP  DDP+ PRPGTL
Sbjct: 64  NPIDDCWRCDPNWETNRQRLADCAIGF-GKNAIG-GRKGRIYVVTDPANDDPVNPRPGTL 123

Query: 90  RYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDGRGATVHITGNACLMVSRATNVII 149
           RY  T  +P +WI F++DM I L K L+I+S+  IDGRG++VHIT   CL +  ATN+II
Sbjct: 124 RYAVTQEEP-LWIIFKRDMVIRLKKELIITSFKTIDGRGSSVHITDGPCLKIHYATNIII 183

Query: 150 HGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLVSASKIWIDHNTLYRCEDGLIDVT 209
           HG+ IH CK  + G +             DGDA+ +     +WIDH +L  C+DGLID  
Sbjct: 184 HGINIHDCKPGSGGMIKDGPHHTGWWMQSDGDAVAIFGGKHVWIDHCSLSNCDDGLIDAI 243

Query: 210 RGSTDITISNNWLRDQDKVILLGHDDDYIWDRNMKII-----WSTGKVENAKWNFHSVRD 269
            GST ITISNN +   DKV+LLGH D Y  D+NM++      +  G V+      H    
Sbjct: 244 HGSTAITISNNHMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFH 303

Query: 270 VFENGASFAQIGAGRGGVKPH-YNALQRFPVVDAKWLRSLTSSSGA 309
           V  N  +  ++ A  G   P  Y+   RF   + ++ + +T    A
Sbjct: 304 VVNNDYTHWEMYAIGGSASPTIYSQGNRFLAPNTRFNKEVTKHEDA 346

[Close]

BLAST of CmaCh01G007690 vs. TAIR 10
Match: AT4G22080.1 (root hair specific 14 )

HSP 1 Score: 216.5 bits (550), Expect = 3.2e-56
Identity = 129/353 (36.54%), Postives = 169/353 (47.88%), Query Frame = 0


Query: 29  MNAIDRCWRSNPYWRKNRQKLALCSIGFVGKMTNNIGRNLVHYQVTDPTDDPLTPRPGTL 88
           MN +D CWR    W  NR+ LA C +GF          NL  Y VT+P D+   P+PG+L
Sbjct: 46  MNPVDSCWRLKSDWAANRKDLADCVVGFGSSTLGGKKGNL--YVVTNPYDNAQNPQPGSL 105

Query: 89  RYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDGRGATVHITGNACLMVSRATNVII 148
           RYG    KP +WITF KDM I L   L+++SY  IDGRGA V I    C+ +   TNVI+
Sbjct: 106 RYGVIQAKP-LWITFAKDMVITLENELMVNSYKTIDGRGAKVEIAYGPCITIQDVTNVIV 165

Query: 149 HGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLVSASKIWIDHNTLYRCEDGLIDVT 208
           HG+ IH CK    G V    + +      DGDAI +  +S IWIDH  L  C DGLIDV 
Sbjct: 166 HGISIHDCKPGKYGMVRSSPTHVGHRKGSDGDAIAIFGSSNIWIDHCYLASCTDGLIDVI 225

Query: 209 RGSTDITISNNWLRDQDKVILLGHDDDYIWDRNMKI------------------------ 268
             ST ITISNN+    DKV+LLGH+DD++ D  MK+                        
Sbjct: 226 HASTGITISNNYFTQHDKVMLLGHNDDFVQDVKMKVTVAFNHFGPGLVERMPRVRRGYAH 285

Query: 269 ---------------------IWSTGKV------ENAK-------------WNFHSVRDV 318
                                I+S G         N+K             W + + +DV
Sbjct: 286 VANNRYDKWIMYAIGGSADPTIFSEGNYFIASDKSNSKEVTKREVKGGWNNWRWRTSKDV 345

The following BLAST results are available for this feature:

BLAST of CmaCh01G007690 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65388	3.3e-58	37.63	Putative pectate lyase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g11920 PE=3 SV=2	[more]
Q9C8G4	2.1e-57	37.17	Probable pectate lyase 4 OS=Arabidopsis thaliana OX=3702 GN=At1g30350 PE=2 SV=1	[more]
Q9LTZ0	3.4e-55	42.66	Putative pectate lyase 11 OS=Arabidopsis thaliana OX=3702 GN=At3g27400 PE=3 SV=2	[more]
O65456	4.4e-55	36.54	Probable pectate lyase 16 OS=Arabidopsis thaliana OX=3702 GN=At4g22080 PE=2 SV=1	[more]
O65457	1.3e-54	35.25	Putative pectate lyase 17 OS=Arabidopsis thaliana OX=3702 GN=At4g22090 PE=3 SV=1	[more]

BLAST of CmaCh01G007690 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KAY1	1.0e-163	82.67	Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111492658 PE=3 SV=1	[more]
A0A6J1E7R9	2.7e-148	68.97	Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111431420 PE=3 SV=1	[more]
A0A6J1KLU6	1.5e-146	68.44	Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111495358 PE=3 SV=1	[more]
A0A5D3BIF3	6.9e-144	69.32	Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00620 ...	[more]
A0A5A7T3T4	2.0e-143	69.32	Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold112G00520 ...	[more]

BLAST of CmaCh01G007690 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7037086.1	5.8e-177	96.85	putative pectate lyase 4, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6607417.1	9.8e-169	87.79	putative pectate lyase 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022997815.1	2.1e-163	82.67	putative pectate lyase 2 isoform X2 [Cucurbita maxima]	[more]
XP_022923821.1	5.6e-148	68.97	putative pectate lyase 2 [Cucurbita moschata]	[more]
KAG7020030.1	7.3e-148	68.70	putative pectate lyase 2, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of CmaCh01G007690 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G09280.1	3.2e-77	69.19	Pectin lyase-like superfamily protein	[more]
AT1G11920.1	2.3e-59	37.63	Pectin lyase-like superfamily protein	[more]
AT1G30350.1	1.5e-58	37.17	Pectin lyase-like superfamily protein	[more]
AT3G27400.1	2.4e-56	42.66	Pectin lyase-like superfamily protein	[more]
AT4G22080.1	3.2e-56	36.54	root hair specific 14	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018082	AmbAllergen	PRINTS	PR00807	AMBALLERGEN	coord: 178..199 score: 51.08 coord: 77..102 score: 42.12 coord: 114..130 score: 51.82
IPR002022	Pectate lyase	SMART	SM00656	amb_all	coord: 106..286 e-value: 6.9E-17 score: 72.1
IPR002022	Pectate lyase	PFAM	PF00544	Pectate_lyase_4	coord: 117..235 e-value: 5.3E-11 score: 42.5
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 29..247 e-value: 1.2E-56 score: 194.2
None	No IPR available	PANTHER	PTHR31683:SF74	PECTATE LYASE	coord: 12..244
None	No IPR available	PANTHER	PTHR31683:SF74	PECTATE LYASE	coord: 244..314
IPR045032	Pectin lyase family	PANTHER	PTHR31683	PECTATE LYASE 18-RELATED	coord: 12..244 coord: 244..314
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 29..278

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh01G007690.1	CmaCh01G007690.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0030570	pectate lyase activity