CmaCh01G007180 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh01G007180 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 8.6e-69
Identity = 185/534 (34.64%), Postives = 287/534 (53.75%), Query Frame = 0

Query: 1   MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF 60
           M  R  +W  LV  IW+Q+  GT+F+F +YSS  K  L +SQ++LN LA ASD GK FG+
Sbjct: 37  MFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGW 96

Query: 61  VSGFAANSLPLWLVLVIGSSLGLVGYGIQYLFIINQLHDPSYWVIFLLTVLAGNSICWIN 120
            SG A    PL +VL   +++G VGYG+Q+L I N +  P Y ++FL  +LAG SICW N
Sbjct: 97  SSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFN 156

Query: 121 TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP 180
           T C+I+ IR+F  +R +A+ ++ S+ G+SA +Y+      F+  +   +  +LLLNS++P
Sbjct: 157 TACFILCIRHFPNNRALALSLTVSFNGISAALYS----LAFNAINPSSSNLYLLLNSLVP 216

Query: 181 LGVC------VFVSPLVRVHVVEEQGGMEIGVFLIF-MITIATGFFAVFTSLGSISTKLS 240
           L V       V   P +      +    +  VF I  ++ + T F  + +S  + S +L+
Sbjct: 217 LVVSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLN 276

Query: 241 PLTGLVGILVF-LLLPLAV------------------------AVAENVKRKTEQPEK-- 300
              G V +LVF L  PL V                         + E   +KT    K  
Sbjct: 277 -FIGAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTG 336

Query: 301 -------HRGGVEAAMEEIGAKEMLKRMNFWLHFGVYFFGATLGLVFLNNLGQIAESRRS 360
                    G      +E   + ++ R+ FWL++  YF G T+GLV+ NNLGQIA+S   
Sbjct: 337 YEHMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQ 396

Query: 361 SGVSSLVSLSSSFGFFGRLTPSLLDHFFPGKLMKSKPGWMVALLGAMCGGFFLLLWPT-- 420
           +  ++LV++ SSF FFGRL  +  D F   +   ++ GW    L      FFLL   +  
Sbjct: 397 NS-TTLVTIYSSFSFFGRLLSAAPD-FMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQ 456

Query: 421 DTSLCTSTAIIAGCTGAITSIAVSVTTELFGADNFSINHNLVVANIPLGSFLFGSLAGFL 480
            T+L T+TA+I   +G I + AVS+T++LFG ++  +NHN+++ NIP+GS L+G +A  +
Sbjct: 457 QTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGYIAASI 516

Query: 481 YR-------NQVPAGAGKCVGVECYRAVFLIWGCLCLVGTLLALVLFARTRSFY 485
           Y          + + +  C+G +CY   F+ WGCL ++G + +L L+ RT+  Y
Sbjct: 517 YEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of CmaCh01G007180 vs. ExPASy TrEMBL
Match: A0A6J1KBR5 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111492867 PE=4 SV=1)

HSP 1 Score: 977.6 bits (2526), Expect = 1.9e-281
Identity = 506/506 (100.00%), Postives = 506/506 (100.00%), Query Frame = 0

Query: 1   MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF 60
           MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF
Sbjct: 1   MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF 60

Query: 61  VSGFAANSLPLWLVLVIGSSLGLVGYGIQYLFIINQLHDPSYWVIFLLTVLAGNSICWIN 120
           VSGFAANSLPLWLVLVIGSSLGLVGYGIQYLFIINQLHDPSYWVIFLLTVLAGNSICWIN
Sbjct: 61  VSGFAANSLPLWLVLVIGSSLGLVGYGIQYLFIINQLHDPSYWVIFLLTVLAGNSICWIN 120

Query: 121 TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP 180
           TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP
Sbjct: 121 TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP 180

Query: 181 LGVCVFVSPLVRVHVVEEQGGMEIGVFLIFMITIATGFFAVFTSLGSISTKLSPLTGLVG 240
           LGVCVFVSPLVRVHVVEEQGGMEIGVFLIFMITIATGFFAVFTSLGSISTKLSPLTGLVG
Sbjct: 181 LGVCVFVSPLVRVHVVEEQGGMEIGVFLIFMITIATGFFAVFTSLGSISTKLSPLTGLVG 240

Query: 241 ILVFLLLPLAVAVAENVKRKTEQPEKHRGGVEAAMEEIGAKEMLKRMNFWLHFGVYFFGA 300
           ILVFLLLPLAVAVAENVKRKTEQPEKHRGGVEAAMEEIGAKEMLKRMNFWLHFGVYFFGA
Sbjct: 241 ILVFLLLPLAVAVAENVKRKTEQPEKHRGGVEAAMEEIGAKEMLKRMNFWLHFGVYFFGA 300

Query: 301 TLGLVFLNNLGQIAESRRSSGVSSLVSLSSSFGFFGRLTPSLLDHFFPGKLMKSKPGWMV 360
           TLGLVFLNNLGQIAESRRSSGVSSLVSLSSSFGFFGRLTPSLLDHFFPGKLMKSKPGWMV
Sbjct: 301 TLGLVFLNNLGQIAESRRSSGVSSLVSLSSSFGFFGRLTPSLLDHFFPGKLMKSKPGWMV 360

Query: 361 ALLGAMCGGFFLLLWPTDTSLCTSTAIIAGCTGAITSIAVSVTTELFGADNFSINHNLVV 420
           ALLGAMCGGFFLLLWPTDTSLCTSTAIIAGCTGAITSIAVSVTTELFGADNFSINHNLVV
Sbjct: 361 ALLGAMCGGFFLLLWPTDTSLCTSTAIIAGCTGAITSIAVSVTTELFGADNFSINHNLVV 420

Query: 421 ANIPLGSFLFGSLAGFLYRNQVPAGAGKCVGVECYRAVFLIWGCLCLVGTLLALVLFART 480
           ANIPLGSFLFGSLAGFLYRNQVPAGAGKCVGVECYRAVFLIWGCLCLVGTLLALVLFART
Sbjct: 421 ANIPLGSFLFGSLAGFLYRNQVPAGAGKCVGVECYRAVFLIWGCLCLVGTLLALVLFART 480

Query: 481 RSFYSTKRRMVEDSPPLHVLPLSLVS 507
           RSFYSTKRRMVEDSPPLHVLPLSLVS
Sbjct: 481 RSFYSTKRRMVEDSPPLHVLPLSLVS 506

BLAST of CmaCh01G007180 vs. ExPASy TrEMBL
Match: A0A6J1GB93 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111452597 PE=4 SV=1)

HSP 1 Score: 944.9 bits (2441), Expect = 1.4e-271
Identity = 489/506 (96.64%), Postives = 495/506 (97.83%), Query Frame = 0

Query: 1   MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF 60
           MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF
Sbjct: 1   MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF 60

Query: 61  VSGFAANSLPLWLVLVIGSSLGLVGYGIQYLFIINQLHDPSYWVIFLLTVLAGNSICWIN 120
           VSGFAANSLPLWLVLVIGSSLGLVGYG+QYLFIIN+LHDPSYWVIFLLTVLAGNSICWIN
Sbjct: 61  VSGFAANSLPLWLVLVIGSSLGLVGYGVQYLFIINKLHDPSYWVIFLLTVLAGNSICWIN 120

Query: 121 TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP 180
           TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP
Sbjct: 121 TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP 180

Query: 181 LGVCVFVSPLVRVHVVEEQGGMEIGVFLIFMITIATGFFAVFTSLGSISTKLSPLTGLVG 240
           LGV V VSP VRVHVVEEQGG+EIGVFLIFMITIATG FAVFTSLGSISTKLSPLTGLVG
Sbjct: 181 LGVSVLVSPFVRVHVVEEQGGLEIGVFLIFMITIATGVFAVFTSLGSISTKLSPLTGLVG 240

Query: 241 ILVFLLLPLAVAVAENVKRKTEQPEKHRGGVEAAMEEIGAKEMLKRMNFWLHFGVYFFGA 300
           ILVFLLLPLAVAVAENVKRKTEQPEK R  VEAAMEEIGAKEMLKRMNFWLHFGVYFFGA
Sbjct: 241 ILVFLLLPLAVAVAENVKRKTEQPEKQRDAVEAAMEEIGAKEMLKRMNFWLHFGVYFFGA 300

Query: 301 TLGLVFLNNLGQIAESRRSSGVSSLVSLSSSFGFFGRLTPSLLDHFFPGKLMKSKPGWMV 360
           TLGLVFLNNLGQIAESRRSSGVSSLVSLS+SFGFFGRL PSLLDHFFPGKLMKSKPGWMV
Sbjct: 301 TLGLVFLNNLGQIAESRRSSGVSSLVSLSASFGFFGRLAPSLLDHFFPGKLMKSKPGWMV 360

Query: 361 ALLGAMCGGFFLLLWPTDTSLCTSTAIIAGCTGAITSIAVSVTTELFGADNFSINHNLVV 420
           ALLGAMCGGFFLLLWPTD SLCTSTAIIAGCTGAITSIAVSVTTELFGADNFSINHNLVV
Sbjct: 361 ALLGAMCGGFFLLLWPTDMSLCTSTAIIAGCTGAITSIAVSVTTELFGADNFSINHNLVV 420

Query: 421 ANIPLGSFLFGSLAGFLYRNQVPAGAGKCVGVECYRAVFLIWGCLCLVGTLLALVLFART 480
           ANIPLGSFLFGSLAGFLYR QVPAGAGKC+GVECYRAVF IWGCLCLVGTLLALVLFART
Sbjct: 421 ANIPLGSFLFGSLAGFLYRMQVPAGAGKCIGVECYRAVFFIWGCLCLVGTLLALVLFART 480

Query: 481 RSFYSTKRRMVEDSPPLHVLPLSLVS 507
           RSFYSTKRRMVEDSPPLHVLPLS+VS
Sbjct: 481 RSFYSTKRRMVEDSPPLHVLPLSIVS 506

BLAST of CmaCh01G007180 vs. ExPASy TrEMBL
Match: A0A6J1I3F0 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469568 PE=4 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 5.4e-199
Identity = 375/512 (73.24%), Postives = 415/512 (81.05%), Query Frame = 0

Query: 1   MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF 60
           M S   QWLSL+GIIWLQSINGT+FNFPSYSSQ KH LS+SQ QLNNLAFASDAGKLF  
Sbjct: 1   MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFAC 60

Query: 61  VSGFAANSLPLWLVLVIGSSLGLVGYGIQYLFIINQLHDPSYWVIFLLTVLAGNSICWIN 120
           VSG AAN LPLW VL+IGSSLGLVGYG+QYLFI NQ H PSY +IF LTVLAGNSICWIN
Sbjct: 61  VSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWIN 120

Query: 121 TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP 180
           TVCYIVAIRNF ++R VAVGISTSYQGLSAKIYTDIV  +FSKRS K  ETFLL NSV P
Sbjct: 121 TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFP 180

Query: 181 LGVCVFVSPLVR-VHVVEEQGGMEIGVFLIFMITIATGFFAVFTSLGSISTKLSPLTGLV 240
           LGVCV VSP+ R V +VEEQG +E+G F+IF+ITIATG FAV TS+GSIS  LSPL+GLV
Sbjct: 181 LGVCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV 240

Query: 241 GILVFLLLPLAVAVAENVKRKTEQ------------------PEK----HRGGVEAAMEE 300
           GILVFLLLP+ V V E+VK+   +                  PE     +R G + AMEE
Sbjct: 241 GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRIRPENEAKLNRDGGDEAMEE 300

Query: 301 IGAKEMLKRMNFWLHFGVYFFGATLGLVFLNNLGQIAESRRSSGVSSLVSLSSSFGFFGR 360
           IGA EMLKR+NFWL+ GVYFFGATLGL FLNNLGQIAESR S  VSSLVSLSSSFGFFGR
Sbjct: 301 IGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLSSSFGFFGR 360

Query: 361 LTPSLLDHFFP-GKLMKSKPGWMVALLGAMCGGFFLLLWPTDTSLCTSTAIIAGCTGAIT 420
           L PSLLDHF    K+MKSKPGW+VAL+GAMCGGFFLL  PTD  LC STA+IA CTGAIT
Sbjct: 361 LMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVSTAVIAVCTGAIT 420

Query: 421 SIAVSVTTELFGADNFSINHNLVVANIPLGSFLFGSLAGFLYRNQVPAGAGKCVGVECYR 480
           SI+VS T+ELFGA+NFSINHN+VVANIP+GSFLFG +A +LYR Q   GAGKC+GVECYR
Sbjct: 421 SISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGGAGKCIGVECYR 480

Query: 481 AVFLIWGCLCLVGTLLALVLFARTRSFYSTKR 489
             F+IWGCLC  GTLLAL+LFARTRSFYST +
Sbjct: 481 TTFMIWGCLCSFGTLLALLLFARTRSFYSTTK 512

BLAST of CmaCh01G007180 vs. ExPASy TrEMBL
Match: A0A6J1HMC6 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464885 PE=4 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 1.3e-192
Identity = 366/513 (71.35%), Postives = 409/513 (79.73%), Query Frame = 0

Query: 1   MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF 60
           M S   QWLSL+GIIWLQSINGT+FNFPSYSS  KH LS+SQ QLNNLAFASDAGKLF  
Sbjct: 55  MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSSPLKHHLSISQFQLNNLAFASDAGKLFAC 114

Query: 61  VSGFAANSLPLWLVLVIGSSLGLVGYGIQYLFIINQLHDPSYWVIFLLTVLAGNSICWIN 120
           VSG AAN LPLW +L+IGS LGLVGYG+QYLFI NQ H PSY +IF L+VLAGNSICWIN
Sbjct: 115 VSGLAANYLPLWSLLIIGSCLGLVGYGVQYLFITNQFHSPSYCLIFFLSVLAGNSICWIN 174

Query: 121 TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP 180
           TVCYIVAIRNF ++R VAVGISTSYQGLSAKIYTDIV  +FSKRS K  ETFLL NSV P
Sbjct: 175 TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFP 234

Query: 181 LGVCVFVSPLVR-VHVVEEQGGMEIGVFLIFMITIATGFFAVFTSLGSISTKLSPLTGLV 240
           LGVCV V P+ R V +VEE+G +E+G F+IF+ITIATG FAV TS+GSIS  LSPL+GLV
Sbjct: 235 LGVCVLVCPMARIVELVEEKGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV 294

Query: 241 GILVFLLLPLAVAVAENVKRKTEQ------------------PEK----HRGGVEAAMEE 300
           GILVFLLLP+ V V E+VK+   +                  PE     +R G + AMEE
Sbjct: 295 GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRIRPENEAKLNRDGGDEAMEE 354

Query: 301 IGAKEMLKRMNFWLHFGVYFFGATLGLVFLNNLGQIAESRRSSGVSSLVSLSSSFGFFGR 360
           IGA EMLKR+NFWL+ GVYFFGATLGL FLNNLGQIAES  S  VSSLVSLSSSFGFFGR
Sbjct: 355 IGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESWGSESVSSLVSLSSSFGFFGR 414

Query: 361 LTPSLLDHFFP-GKLMKSKPGWMVALLGAMCGGFFLLL-WPTDTSLCTSTAIIAGCTGAI 420
           L PSLLDHF    K+MKSKPGW+VAL+GAMCGGFF LL  PTD  LC STA+IA CTGAI
Sbjct: 415 LMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFFLLSGPTDLILCVSTAVIAVCTGAI 474

Query: 421 TSIAVSVTTELFGADNFSINHNLVVANIPLGSFLFGSLAGFLYRNQVPAGAGKCVGVECY 480
           TSI+VS T+ELFGA+NFSINHN+VVANIP GSFLFG +A +LYR Q     GKC+GVECY
Sbjct: 475 TSISVSTTSELFGANNFSINHNVVVANIPFGSFLFGYMAAYLYRKQATGDTGKCIGVECY 534

Query: 481 RAVFLIWGCLCLVGTLLALVLFARTRSFYSTKR 489
           R  F+IWGCLC +GTLLAL+LFARTRSFYST +
Sbjct: 535 RTTFMIWGCLCSLGTLLALLLFARTRSFYSTTK 567

BLAST of CmaCh01G007180 vs. ExPASy TrEMBL
Match: A0A1S3C569 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103497028 PE=4 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 2.9e-192
Identity = 373/518 (72.01%), Postives = 412/518 (79.54%), Query Frame = 0

Query: 2   SSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGFV 61
           SS   QWLSL+GIIWLQSINGT+FNFPSYSSQ K QLSMSQLQLNNLAFASDAGKLF   
Sbjct: 4   SSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACF 63

Query: 62  SGFAANSLPLWLVLVIGSSLGLVGYGIQYLFIINQLHDPSYWVIFLLTVLAGNSICWINT 121
           SG AAN LPLWLVL IGSSLGL+GYG+QYLFI NQ H PSYW+IF LTVLAGNSICWINT
Sbjct: 64  SGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWINT 123

Query: 122 VCYIVAIRNF-KAHRHVAVGISTSYQGLSAKIYTDIVESMFS-KRSSKRTETFLLLNSVL 181
           VCY+VAI NF  ++R VAVGISTSYQGLSAK+YTDIV S+FS K SSK  ETFLLLNSVL
Sbjct: 124 VCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLNSVL 183

Query: 182 PLGVCVFVSPLVR-VHVVEEQGGMEIGVFLIFMITIATGFFAVFTSLGSISTKLSPLTGL 241
           PLGVCV VSPL R V +VEEQG +E+G F+IF+ITIATG FA  TS+GS+S  LS L+GL
Sbjct: 184 PLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSALSGL 243

Query: 242 VGILVFLLLPLAVAVAENVKRKTEQPEKHR---------GGVE----------------- 301
           VGI+VFL+LPL V VAE VK + E+ ++ R           VE                 
Sbjct: 244 VGIMVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEENNDVERIMRGENERKVERTDEG 303

Query: 302 AAMEEIGAKEMLKRMNFWLHFGVYFFGATLGLVFLNNLGQIAESRRSSGVSSLVSLSSSF 361
            AMEEIGAKEM+KR+NFWL+FGVY FGATLGL FLNNLGQIAESR SS VSSLVSLSSSF
Sbjct: 304 EAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF 363

Query: 362 GFFGRLTPSLLDHFFP--GKLMKSKPGWMVALLGAMCGGFFLLLWPTDTSLCTSTAIIAG 421
           GFFGRL PS+LD+F     K MKSKPGWMV L+GA+CGGFFLLL P+DTSLC STAIIA 
Sbjct: 364 GFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTAIIAI 423

Query: 422 CTGAITSIAVSVTTELFGADNFSINHNLVVANIPLGSFLFGSLAGFLYRNQVPAG--AGK 481
           CTGAITSI+VS TT+LFGA NFSINHN+VVANIP GSF+FG +A FLY  Q   G  AGK
Sbjct: 424 CTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVAAGK 483

Query: 482 CVGVECYRAVFLIWGCLCLVGTLLALVLFARTRSFYST 487
           C+GVECYR  FLIWGC    GT LAL+LFARTRSFYST
Sbjct: 484 CIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYST 521

BLAST of CmaCh01G007180 vs. NCBI nr
Match: XP_022998130.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 977.6 bits (2526), Expect = 4.0e-281
Identity = 506/506 (100.00%), Postives = 506/506 (100.00%), Query Frame = 0

Query: 1   MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF 60
           MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF
Sbjct: 1   MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF 60

Query: 61  VSGFAANSLPLWLVLVIGSSLGLVGYGIQYLFIINQLHDPSYWVIFLLTVLAGNSICWIN 120
           VSGFAANSLPLWLVLVIGSSLGLVGYGIQYLFIINQLHDPSYWVIFLLTVLAGNSICWIN
Sbjct: 61  VSGFAANSLPLWLVLVIGSSLGLVGYGIQYLFIINQLHDPSYWVIFLLTVLAGNSICWIN 120

Query: 121 TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP 180
           TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP
Sbjct: 121 TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP 180

Query: 181 LGVCVFVSPLVRVHVVEEQGGMEIGVFLIFMITIATGFFAVFTSLGSISTKLSPLTGLVG 240
           LGVCVFVSPLVRVHVVEEQGGMEIGVFLIFMITIATGFFAVFTSLGSISTKLSPLTGLVG
Sbjct: 181 LGVCVFVSPLVRVHVVEEQGGMEIGVFLIFMITIATGFFAVFTSLGSISTKLSPLTGLVG 240

Query: 241 ILVFLLLPLAVAVAENVKRKTEQPEKHRGGVEAAMEEIGAKEMLKRMNFWLHFGVYFFGA 300
           ILVFLLLPLAVAVAENVKRKTEQPEKHRGGVEAAMEEIGAKEMLKRMNFWLHFGVYFFGA
Sbjct: 241 ILVFLLLPLAVAVAENVKRKTEQPEKHRGGVEAAMEEIGAKEMLKRMNFWLHFGVYFFGA 300

Query: 301 TLGLVFLNNLGQIAESRRSSGVSSLVSLSSSFGFFGRLTPSLLDHFFPGKLMKSKPGWMV 360
           TLGLVFLNNLGQIAESRRSSGVSSLVSLSSSFGFFGRLTPSLLDHFFPGKLMKSKPGWMV
Sbjct: 301 TLGLVFLNNLGQIAESRRSSGVSSLVSLSSSFGFFGRLTPSLLDHFFPGKLMKSKPGWMV 360

Query: 361 ALLGAMCGGFFLLLWPTDTSLCTSTAIIAGCTGAITSIAVSVTTELFGADNFSINHNLVV 420
           ALLGAMCGGFFLLLWPTDTSLCTSTAIIAGCTGAITSIAVSVTTELFGADNFSINHNLVV
Sbjct: 361 ALLGAMCGGFFLLLWPTDTSLCTSTAIIAGCTGAITSIAVSVTTELFGADNFSINHNLVV 420

Query: 421 ANIPLGSFLFGSLAGFLYRNQVPAGAGKCVGVECYRAVFLIWGCLCLVGTLLALVLFART 480
           ANIPLGSFLFGSLAGFLYRNQVPAGAGKCVGVECYRAVFLIWGCLCLVGTLLALVLFART
Sbjct: 421 ANIPLGSFLFGSLAGFLYRNQVPAGAGKCVGVECYRAVFLIWGCLCLVGTLLALVLFART 480

Query: 481 RSFYSTKRRMVEDSPPLHVLPLSLVS 507
           RSFYSTKRRMVEDSPPLHVLPLSLVS
Sbjct: 481 RSFYSTKRRMVEDSPPLHVLPLSLVS 506

BLAST of CmaCh01G007180 vs. NCBI nr
Match: XP_022949166.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 944.9 bits (2441), Expect = 2.9e-271
Identity = 489/506 (96.64%), Postives = 495/506 (97.83%), Query Frame = 0

Query: 1   MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF 60
           MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF
Sbjct: 1   MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF 60

Query: 61  VSGFAANSLPLWLVLVIGSSLGLVGYGIQYLFIINQLHDPSYWVIFLLTVLAGNSICWIN 120
           VSGFAANSLPLWLVLVIGSSLGLVGYG+QYLFIIN+LHDPSYWVIFLLTVLAGNSICWIN
Sbjct: 61  VSGFAANSLPLWLVLVIGSSLGLVGYGVQYLFIINKLHDPSYWVIFLLTVLAGNSICWIN 120

Query: 121 TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP 180
           TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP
Sbjct: 121 TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP 180

Query: 181 LGVCVFVSPLVRVHVVEEQGGMEIGVFLIFMITIATGFFAVFTSLGSISTKLSPLTGLVG 240
           LGV V VSP VRVHVVEEQGG+EIGVFLIFMITIATG FAVFTSLGSISTKLSPLTGLVG
Sbjct: 181 LGVSVLVSPFVRVHVVEEQGGLEIGVFLIFMITIATGVFAVFTSLGSISTKLSPLTGLVG 240

Query: 241 ILVFLLLPLAVAVAENVKRKTEQPEKHRGGVEAAMEEIGAKEMLKRMNFWLHFGVYFFGA 300
           ILVFLLLPLAVAVAENVKRKTEQPEK R  VEAAMEEIGAKEMLKRMNFWLHFGVYFFGA
Sbjct: 241 ILVFLLLPLAVAVAENVKRKTEQPEKQRDAVEAAMEEIGAKEMLKRMNFWLHFGVYFFGA 300

Query: 301 TLGLVFLNNLGQIAESRRSSGVSSLVSLSSSFGFFGRLTPSLLDHFFPGKLMKSKPGWMV 360
           TLGLVFLNNLGQIAESRRSSGVSSLVSLS+SFGFFGRL PSLLDHFFPGKLMKSKPGWMV
Sbjct: 301 TLGLVFLNNLGQIAESRRSSGVSSLVSLSASFGFFGRLAPSLLDHFFPGKLMKSKPGWMV 360

Query: 361 ALLGAMCGGFFLLLWPTDTSLCTSTAIIAGCTGAITSIAVSVTTELFGADNFSINHNLVV 420
           ALLGAMCGGFFLLLWPTD SLCTSTAIIAGCTGAITSIAVSVTTELFGADNFSINHNLVV
Sbjct: 361 ALLGAMCGGFFLLLWPTDMSLCTSTAIIAGCTGAITSIAVSVTTELFGADNFSINHNLVV 420

Query: 421 ANIPLGSFLFGSLAGFLYRNQVPAGAGKCVGVECYRAVFLIWGCLCLVGTLLALVLFART 480
           ANIPLGSFLFGSLAGFLYR QVPAGAGKC+GVECYRAVF IWGCLCLVGTLLALVLFART
Sbjct: 421 ANIPLGSFLFGSLAGFLYRMQVPAGAGKCIGVECYRAVFFIWGCLCLVGTLLALVLFART 480

Query: 481 RSFYSTKRRMVEDSPPLHVLPLSLVS 507
           RSFYSTKRRMVEDSPPLHVLPLS+VS
Sbjct: 481 RSFYSTKRRMVEDSPPLHVLPLSIVS 506

BLAST of CmaCh01G007180 vs. NCBI nr
Match: KAG6607357.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 944.9 bits (2441), Expect = 2.9e-271
Identity = 491/512 (95.90%), Postives = 497/512 (97.07%), Query Frame = 0

Query: 1   MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF 60
           MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF
Sbjct: 1   MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF 60

Query: 61  VSGFAANSLPLWLVLVIGSSLGLVGYGIQYLFIINQLHDPSYWVIFLLTVLAGNSICWIN 120
           VSGFAANSLPLWLVLVIGSSLGLVGYG+QYLFIIN+LHDPSYWVIFLLTVLAGNSICWIN
Sbjct: 61  VSGFAANSLPLWLVLVIGSSLGLVGYGVQYLFIINKLHDPSYWVIFLLTVLAGNSICWIN 120

Query: 121 TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP 180
           TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP
Sbjct: 121 TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP 180

Query: 181 LGVCVFVSPLVRVHVVEEQGGMEIGVFLIFMITIATGFFAVFTSLGSISTKLSPLTGLVG 240
           LGVCV VSP VRVHVVEEQGG+EIGVFLIFMITIATGFFAVFTSLGSISTKLSPLTGLVG
Sbjct: 181 LGVCVLVSPFVRVHVVEEQGGLEIGVFLIFMITIATGFFAVFTSLGSISTKLSPLTGLVG 240

Query: 241 ILVFLLLPLAVAVAENVKR------KTEQPEKHRGGVEAAMEEIGAKEMLKRMNFWLHFG 300
           ILVFLLLPLAVAVAENVKR      KTEQPEK R  VEAAMEEIGAKEMLKRMNFWLHFG
Sbjct: 241 ILVFLLLPLAVAVAENVKRKTEQPEKTEQPEKQRDAVEAAMEEIGAKEMLKRMNFWLHFG 300

Query: 301 VYFFGATLGLVFLNNLGQIAESRRSSGVSSLVSLSSSFGFFGRLTPSLLDHFFPGKLMKS 360
           VYFFGATLGLVFLNNLGQIAESRRSSGVSSLVSLS+SFGFFGRL PSLLDHFFPGKLMKS
Sbjct: 301 VYFFGATLGLVFLNNLGQIAESRRSSGVSSLVSLSASFGFFGRLAPSLLDHFFPGKLMKS 360

Query: 361 KPGWMVALLGAMCGGFFLLLWPTDTSLCTSTAIIAGCTGAITSIAVSVTTELFGADNFSI 420
           KPGWMVALLGAMCGGFFLLLWPTD SLCTSTAIIAGCTGAITSIAVSVTTELFGADNFSI
Sbjct: 361 KPGWMVALLGAMCGGFFLLLWPTDMSLCTSTAIIAGCTGAITSIAVSVTTELFGADNFSI 420

Query: 421 NHNLVVANIPLGSFLFGSLAGFLYRNQVPAGAGKCVGVECYRAVFLIWGCLCLVGTLLAL 480
           NHNLVVANIPLGSFLFGSLAGFLYR QVPAGAGKC+GVECYRAVF IWGCLCLVGTLLAL
Sbjct: 421 NHNLVVANIPLGSFLFGSLAGFLYRMQVPAGAGKCIGVECYRAVFFIWGCLCLVGTLLAL 480

Query: 481 VLFARTRSFYSTKRRMVEDSPPLHVLPLSLVS 507
           VLFARTRSFYSTKRRMVEDSPPLHVLPLS+VS
Sbjct: 481 VLFARTRSFYSTKRRMVEDSPPLHVLPLSIVS 512

BLAST of CmaCh01G007180 vs. NCBI nr
Match: XP_023525436.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 943.3 bits (2437), Expect = 8.4e-271
Identity = 485/506 (95.85%), Postives = 494/506 (97.63%), Query Frame = 0

Query: 1   MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF 60
           MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF
Sbjct: 1   MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF 60

Query: 61  VSGFAANSLPLWLVLVIGSSLGLVGYGIQYLFIINQLHDPSYWVIFLLTVLAGNSICWIN 120
           VSGFAANSLPLWLVLVIGSSLGLVGYG+QYLFIIN+LHDPSYWVIFLLTVL+GNSICWIN
Sbjct: 61  VSGFAANSLPLWLVLVIGSSLGLVGYGVQYLFIINKLHDPSYWVIFLLTVLSGNSICWIN 120

Query: 121 TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP 180
           TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSS RTETFLLLNSVLP
Sbjct: 121 TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSNRTETFLLLNSVLP 180

Query: 181 LGVCVFVSPLVRVHVVEEQGGMEIGVFLIFMITIATGFFAVFTSLGSISTKLSPLTGLVG 240
           LGVCV VSP VRVHV+EEQGG+EIGVF IFMITIATGFFAVFTSLGSISTKLSPLTGLVG
Sbjct: 181 LGVCVLVSPFVRVHVMEEQGGLEIGVFFIFMITIATGFFAVFTSLGSISTKLSPLTGLVG 240

Query: 241 ILVFLLLPLAVAVAENVKRKTEQPEKHRGGVEAAMEEIGAKEMLKRMNFWLHFGVYFFGA 300
           ILVFLLLPLAVAVAENVKRKTEQPEK R  VE AMEEIGAKEMLKRMNFWLHFGVYFFGA
Sbjct: 241 ILVFLLLPLAVAVAENVKRKTEQPEKQRDAVETAMEEIGAKEMLKRMNFWLHFGVYFFGA 300

Query: 301 TLGLVFLNNLGQIAESRRSSGVSSLVSLSSSFGFFGRLTPSLLDHFFPGKLMKSKPGWMV 360
           TLGLVFLNNLGQIAESRRSSGVSSLVSLS+SFGFFGRLTPSLLDHFFPGKLMKSKPGWMV
Sbjct: 301 TLGLVFLNNLGQIAESRRSSGVSSLVSLSASFGFFGRLTPSLLDHFFPGKLMKSKPGWMV 360

Query: 361 ALLGAMCGGFFLLLWPTDTSLCTSTAIIAGCTGAITSIAVSVTTELFGADNFSINHNLVV 420
           ALLGAMCGGFFLLLWPTD SLCTSTAIIAGCTGAITSIAVSVTTELFGADNFSINHNLVV
Sbjct: 361 ALLGAMCGGFFLLLWPTDMSLCTSTAIIAGCTGAITSIAVSVTTELFGADNFSINHNLVV 420

Query: 421 ANIPLGSFLFGSLAGFLYRNQVPAGAGKCVGVECYRAVFLIWGCLCLVGTLLALVLFART 480
           ANIPLGSFLFGSLAGFLYR QVPAG GKC+GVECYRAVF IWGCLCLVGTLLALVLFART
Sbjct: 421 ANIPLGSFLFGSLAGFLYRMQVPAGTGKCIGVECYRAVFFIWGCLCLVGTLLALVLFART 480

Query: 481 RSFYSTKRRMVEDSPPLHVLPLSLVS 507
           RSFYSTKRRMVED+PPLHVLPLS+VS
Sbjct: 481 RSFYSTKRRMVEDAPPLHVLPLSIVS 506

BLAST of CmaCh01G007180 vs. NCBI nr
Match: KAG7037031.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 939.1 bits (2426), Expect = 1.6e-269
Identity = 489/512 (95.51%), Postives = 495/512 (96.68%), Query Frame = 0

Query: 1   MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF 60
           MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF
Sbjct: 1   MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF 60

Query: 61  VSGFAANSLPLWLVLVIGSSLGLVGYGIQYLFIINQLHDPSYWVIFLLTVLAGNSICWIN 120
           VSGFAANSLPLWLVLVIGSSLGLVGYG+QYLFI N+LHDPSYWVIFLLTVLAGNSICWIN
Sbjct: 61  VSGFAANSLPLWLVLVIGSSLGLVGYGVQYLFITNKLHDPSYWVIFLLTVLAGNSICWIN 120

Query: 121 TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP 180
           TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP
Sbjct: 121 TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP 180

Query: 181 LGVCVFVSPLVRVHVVEEQGGMEIGVFLIFMITIATGFFAVFTSLGSISTKLSPLTGLVG 240
           LGV V VSP VRVHVVEEQGG+EIGVFLIFMITIATGFFAVFTSLGSISTKLSPLTGLVG
Sbjct: 181 LGVSVLVSPFVRVHVVEEQGGLEIGVFLIFMITIATGFFAVFTSLGSISTKLSPLTGLVG 240

Query: 241 ILVFLLLPLAVAVAENVKR------KTEQPEKHRGGVEAAMEEIGAKEMLKRMNFWLHFG 300
           ILVFLLLPLAVAVAENVKR      KTEQPEK R  VEAAMEEIGAKEMLKRMNFWLHFG
Sbjct: 241 ILVFLLLPLAVAVAENVKRKTEQPEKTEQPEKQRDAVEAAMEEIGAKEMLKRMNFWLHFG 300

Query: 301 VYFFGATLGLVFLNNLGQIAESRRSSGVSSLVSLSSSFGFFGRLTPSLLDHFFPGKLMKS 360
           VYFFGATLGLVFLNNLGQIAESRRSSGVSSLVSLS+SFGFFGRL PSLLDHFFPGKLMKS
Sbjct: 301 VYFFGATLGLVFLNNLGQIAESRRSSGVSSLVSLSASFGFFGRLAPSLLDHFFPGKLMKS 360

Query: 361 KPGWMVALLGAMCGGFFLLLWPTDTSLCTSTAIIAGCTGAITSIAVSVTTELFGADNFSI 420
           KPGWMVALLGAMCGGFFLLLWPTD SLCTSTAIIAGCTGAITSIAVSVTTELFGADNFSI
Sbjct: 361 KPGWMVALLGAMCGGFFLLLWPTDMSLCTSTAIIAGCTGAITSIAVSVTTELFGADNFSI 420

Query: 421 NHNLVVANIPLGSFLFGSLAGFLYRNQVPAGAGKCVGVECYRAVFLIWGCLCLVGTLLAL 480
           NHNLVVANIPLGSFLFGSLAGFLYR QVPAGAGKC+GVECYRAVF IWGCLCLVGTLLAL
Sbjct: 421 NHNLVVANIPLGSFLFGSLAGFLYRMQVPAGAGKCIGVECYRAVFFIWGCLCLVGTLLAL 480

Query: 481 VLFARTRSFYSTKRRMVEDSPPLHVLPLSLVS 507
           VLFARTRSFYSTKRRMVEDSPPLHVLPLS+VS
Sbjct: 481 VLFARTRSFYSTKRRMVEDSPPLHVLPLSIVS 512

BLAST of CmaCh01G007180 vs. TAIR 10
Match: AT2G30300.1 (Major facilitator superfamily protein )

HSP 1 Score: 418.3 bits (1074), Expect = 8.7e-117
Identity = 246/521 (47.22%), Postives = 323/521 (62.00%), Query Frame = 0

Query: 1   MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF 60
           MS + L WLSLV I+WLQS NGT+ +FP+YSSQ K  L +SQ +LN L+FASDAGK+ GF
Sbjct: 1   MSRKILPWLSLVAILWLQSFNGTNLSFPAYSSQLKELLKISQFKLNYLSFASDAGKVLGF 60

Query: 61  VSGFAANSLPLWLVLVIGSSLGLVGYGIQYLFIINQLHDPSYWVIFLLTVLAGNSICWIN 120
           +SG AA  LPL LVL+ G SLG  GYG+QYL II ++                  ICWIN
Sbjct: 61  ISGIAAVYLPLPLVLLAGGSLGFAGYGLQYLSIIKKI------------------ICWIN 120

Query: 121 TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP 180
           T CYIVAI +F  +R VAVGI+ SYQGLS KIYTD+V S F     +    +LLLNS++P
Sbjct: 121 TACYIVAINSFPVNRQVAVGITASYQGLSGKIYTDMVHSFFHTSQREEASGYLLLNSLVP 180

Query: 181 LGVCVFVSPLVRVH----VVEEQGGMEIGVFLIFMITIATGFFAVFTSLGSISTKLSPLT 240
           L  C+  +P++  H     +     +++G  ++F++TIATG +AV TSL S+   L    
Sbjct: 181 LVACLVTAPMLMRHGGDKTMSYSKDVKVGFIVLFVLTIATGIYAVATSLVSVPAVLV--- 240

Query: 241 GLVGILVFLLLPLAVAVAENVK-----RKTEQ----------------------PEKHRG 300
            LVGI +FLL PLA+ +    K     RKT+Q                       E+   
Sbjct: 241 -LVGIALFLLAPLAIPIGVGFKELMSSRKTQQKVHDLEAPVDKFYFVEEDHTKEEEEFEK 300

Query: 301 GVEAAMEEIGAKEMLKRMNFWLHFGVYFFGATLGLVFLNNLGQIAESRRSSGVSSLVSLS 360
            +    EE+   ++ K+++FW++FG+Y FG T+GLVF NNLGQIAESR S+  SSLV+LS
Sbjct: 301 AIIGVKEEVEWTQLWKKLDFWIYFGLYLFGPTVGLVFTNNLGQIAESRGSTATSSLVALS 360

Query: 361 SSFGFFGRLTPSLLDHFFP-GKLMKSKPGWMVALLGAMCGGFFLLLWPTDTSLCTSTAII 420
           SSFGFFGRL PSLLD+FF   K M S P  M   L AM   F LLL  +D +L   TA+I
Sbjct: 361 SSFGFFGRLLPSLLDYFFSRNKYMPSSPVSMAGSLVAMVASFLLLLIDSDIALYIGTAMI 420

Query: 421 AGCTGAITSIAVSVTTELFGADNFSINHNLVVANIPLGSFLFGSLAGFLYRN--QVPAGA 480
              +GA+TS++V++T ELFG  +F +NHN+VV +IPLGSF FG LA  +YR+   +    
Sbjct: 421 GIFSGALTSLSVTMTAELFGTKHFGVNHNIVVGSIPLGSFSFGLLAAKIYRDGAALYGDD 480

Query: 481 GKCVGVECYRAVFLIWGCLCLVGTLLALVLFARTRSFYSTK 488
           GKC G+ C++   + WG LC +  LLA VL+ R R FYS K
Sbjct: 481 GKCFGMHCFQTTLVFWGMLCSIAALLAAVLYIRNRKFYSQK 499

BLAST of CmaCh01G007180 vs. TAIR 10
Match: AT3G01630.1 (Major facilitator superfamily protein )

HSP 1 Score: 274.2 bits (700), Expect = 2.0e-73
Identity = 189/526 (35.93%), Postives = 289/526 (54.94%), Query Frame = 0

Query: 7   QWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGFVSGFAA 66
           +W  LV  IW+Q+  GT+F+F +YSS  K  + +SQ +LN +A ASD GK  G+ SGFA 
Sbjct: 23  RWTVLVAAIWIQAFTGTNFDFSAYSSDMKSSMGVSQSRLNYMAVASDLGKALGWSSGFAI 82

Query: 67  NSLPLWLVLVIGSSLGLVGYGIQYLFIINQLHDPSYWVIFLLTVLAGNSICWINTVCYIV 126
              P+  VL   +++GLVGYG+Q+L I + + D  Y ++ +   LAG SICW NTV +I+
Sbjct: 83  AYFPVSGVLFAAAAMGLVGYGVQWLSIADVI-DLPYSLVLVCCSLAGLSICWFNTVSFIL 142

Query: 127 AIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLPLGVCVF 186
            IR+FKA+  +A+ +  S+ G+SA +YT +     S +SS  ++ +LLLNS++PL V V 
Sbjct: 143 CIRHFKANHSLALSLVVSFNGISAALYT-LGHEAISGKSSASSDIYLLLNSLIPLIVSVL 202

Query: 187 VSPLVRVHVVEEQGGM-----EIGVFLIF-MITIATGFFAVFTSLGSISTKLSPLTGLVG 246
               V  +    +        E  +F++F ++ + T F+ +  S G+     SP    +G
Sbjct: 203 ALWPVLTNPNSSETDTTRTHDETRIFVVFNVLALVTCFYLLLPSSGTYLAS-SPRWHFLG 262

Query: 247 ILVFLLLPLAVAVAENVKRKTEQPEKHRGGVEAAM------------EEIGAKE------ 306
            +  LL PL V   + + R  E    H     AA+            ++  A+E      
Sbjct: 263 AIFLLLFPLCVPFLDYIHRALESCFHHHSSGYAAVNIEEPKILKIKSQKSNAEEESDQVR 322

Query: 307 ---------MLKRMNFWLHFGVYFFGATLGLVFLNNLGQIAES--RRSSGVSSLVSLSSS 366
                    +++++ FWL++  YF G T+GLV+ NNLGQIA+S  + SS   SLV+L S+
Sbjct: 323 LGDEHSLGMLVRKLEFWLYYVAYFCGGTIGLVYSNNLGQIAQSLGQSSSNAKSLVTLFSA 382

Query: 367 FGFFGRLTPSLLDHFFPGKLMK--SKPGWMVALLGAMCGGFFLLLWPTDTS----LCTST 426
           F F GRL  S  D  F  K +K  ++ GW    L      FF+L +   T+    L  +T
Sbjct: 383 FSFLGRLLSSAPD--FTRKKLKYLTRTGWFTISLLPTPLAFFILAYSPKTNQTALLEVAT 442

Query: 427 AIIAGCTGAITSIAVSVTTELFGADNFSINHNLVVANIPLGSFLFGSLAGFLY------- 485
           A+I   +G + + AVS+T+ELFG ++  +N N+++ NIP+GS  +G +AG +Y       
Sbjct: 443 ALIGLSSGFVFAAAVSITSELFGRNSVGVNQNILITNIPIGSLFYGYMAGSVYDTNASLG 502

BLAST of CmaCh01G007180 vs. TAIR 10
Match: AT5G45275.1 (Major facilitator superfamily protein )

HSP 1 Score: 274.2 bits (700), Expect = 2.0e-73
Identity = 199/563 (35.35%), Postives = 304/563 (54.00%), Query Frame = 0

Query: 1   MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF 60
           M+ ++ +W+ LV  IW+Q+  GT+F+F +YSS  K  L +SQ+QLN LA ASD GK+FG+
Sbjct: 1   MAGQSRKWMILVATIWIQAFTGTNFDFSTYSSNLKSVLGISQVQLNYLAVASDLGKVFGW 60

Query: 61  VSGFAANSLPLWLVLVIGSSLGLVGYGIQYLFIINQLHDPSYWVIFLLTVLAGNSICWIN 120
            SG A    PLW VL   + +G VGYG+Q+L I N +  P Y ++FL  +LAG SICW N
Sbjct: 61  SSGLALLYFPLWTVLFAAAIMGFVGYGVQWLVITNVISLP-YILVFLCCLLAGLSICWFN 120

Query: 121 TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP 180
           TVC+++ IRNF A+R +A+ ++ S+ G+SA +YT      ++  +   TE +LLLN+++P
Sbjct: 121 TVCFVLCIRNFPANRSLALSLTVSFNGVSAALYT----LAYNAINPVSTELYLLLNALVP 180

Query: 181 LGVC-VFVSPLVRVHVVE--EQGGM---EIGVFLIFMITIATG-FFAVFTSLGSISTKLS 240
           L V    + P++R   +E     G+    +   L+ ++ +  G +  +F S  S  T   
Sbjct: 181 LFVSFAALIPILRQPPLEPLPPDGVRRDSLMFLLLNILAVLNGVYLLLFRSKTSDVTSAR 240

Query: 241 PLTGLVGILVFLLLPL---AVAVAEN-----------------VKRKTEQPEKHRGGV-- 300
            L G  G ++ L+LPL    +  A N                 +    ++ E H+G V  
Sbjct: 241 LLFG--GSILLLILPLCLPGLVYARNWYLHNIHSSFRLEGSGFILVDVDELEMHKGMVTR 300

Query: 301 EAAM--------------------------------------------EEIGAKEMLKRM 360
           EA++                                            EE     +L R 
Sbjct: 301 EASLEGYQLLNDDVVRAVNTPDQKSFIEDDDGCCCTKVITRNQLGMLGEEHPLSFLLCRS 360

Query: 361 NFWLHFGVYFFGATLGLVFLNNLGQIAES-RRSSGVSSLVSLSSSFGFFGRLTPSLLDHF 420
           +FWL++  YF G T+GLV+ NNLGQIA+S  +SS  ++LV+L SSF FFGRL  +  D +
Sbjct: 361 DFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQSSETTTLVTLYSSFSFFGRLLSATPD-Y 420

Query: 421 FPGKLMKSKPGWM-VALLGAMCGGFFLLLWPTDTSLCTSTAIIAGCTGAITSIAVSVTTE 480
              K+  ++ GW+ VALL      F L    +  +L   TA+I   +G I + AVS+T+E
Sbjct: 421 IRAKVYFARTGWLAVALLPTTIALFLLASSGSLAALQAGTALIGLSSGFIFAAAVSITSE 480

Query: 481 LFGADNFSINHNLVVANIPLGSFLFGSLAGFLYRNQVPAGAGK----CVGVECYRAVFLI 485
           LFG ++  +NHN+++ NIP+GS ++G LA  +Y +   AG+      C+G +CY   F+ 
Sbjct: 481 LFGPNSVGVNHNILITNIPIGSLVYGFLAALVYESHSVAGSKTESVICMGRDCYLQTFMW 540

BLAST of CmaCh01G007180 vs. TAIR 10
Match: AT4G19450.1 (Major facilitator superfamily protein )

HSP 1 Score: 270.0 bits (689), Expect = 3.8e-72
Identity = 195/563 (34.64%), Postives = 299/563 (53.11%), Query Frame = 0

Query: 1   MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF 60
           M+ ++ +W+ LV  IW+Q+  GT+F+F +YSS  K  L +SQ+QLN LA ASD GK+FG+
Sbjct: 1   MAGQSRKWMILVATIWIQAFTGTNFDFSAYSSDLKSVLGISQVQLNYLAVASDLGKVFGW 60

Query: 61  VSGFAANSLPLWLVLVIGSSLGLVGYGIQYLFIINQLHDPSYWVIFLLTVLAGNSICWIN 120
            SG A    PLW VL   + +G VGYG+Q+L I + +  P Y ++FL  +LAG SICW N
Sbjct: 61  SSGLALMYFPLWTVLFAAAFMGFVGYGVQWLVITHFISLP-YIMVFLCCLLAGLSICWFN 120

Query: 121 TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP 180
           TVC+++ I NF A+R +A+ ++ S+ G+SA +YT      ++  +    E +LLLN+++P
Sbjct: 121 TVCFVLCISNFPANRSLALSLTVSFNGVSAALYT----LAYNAINPTSPELYLLLNALIP 180

Query: 181 LGVC-VFVSPLVRVHVVE--EQGGMEIGVFLIFMITIATGFFAVFTSL-GSISTKLSPLT 240
           L V    + P++R    E     G+     +  ++ I      V+  L GS S+ L+   
Sbjct: 181 LIVSFTAIIPILRQPPFEPLPPDGVRRDSLMFLLLNILAALNGVYLLLFGSNSSDLTSAR 240

Query: 241 GLVG----ILVFLLLPLAVAVAENVKRKT-----------------EQPEKHRGGV---- 300
            L G    +LVF L    + +A N   +T                 ++ E H+G +    
Sbjct: 241 LLFGGAILLLVFPLCIPGLVIARNWYNRTIHTSFRLEGSGFILVDPDELELHKGMLAHEA 300

Query: 301 ------------------EAAMEEIGAKE-----------------------MLKRMNFW 360
                               A+EE  + E                       +L R +FW
Sbjct: 301 NREGYQLLSDDVVQNPVKSVAVEEEDSDESCCKKLITRDQLEGLGIEHSLSLLLTRSDFW 360

Query: 361 LHFGVYFFGATLGLVFLNNLGQIAES-RRSSGVSSLVSLSSSFGFFGRLTPSLLDHFFPG 420
           L++  YF G T+GLV+ NNLGQIA+S  +SS  ++LV+L S+F FFGRL  +  D +   
Sbjct: 361 LYYITYFCGGTIGLVYSNNLGQIAQSLGQSSNTTTLVTLYSAFSFFGRLLSATPD-YIRA 420

Query: 421 KLMKSKPGWM-VALLGAMCGGFFLLLWPTDTSLCTSTAIIAGCTGAITSIAVSVTTELFG 480
           K+  ++ GW+ +ALL      F L    T ++L   TA++   +G I + AVS+T+ELFG
Sbjct: 421 KVYFARTGWLAIALLPTPFALFLLASSGTASALQAGTALMGLSSGFIFAAAVSITSELFG 480

Query: 481 ADNFSINHNLVVANIPLGSFLFGSLAGFLYRNQVPAGAGK-------CVGVECYRAVFLI 485
            ++  +NHN+++ NIP+GS ++G LA  +Y +    G          C+G +CY   F+ 
Sbjct: 481 PNSVGVNHNILITNIPIGSLIYGFLAALVYDSHGFTGTKSMTSESVVCMGRDCYYLTFVW 540

BLAST of CmaCh01G007180 vs. TAIR 10
Match: AT1G31470.1 (Major facilitator superfamily protein )

HSP 1 Score: 262.7 bits (670), Expect = 6.1e-70
Identity = 185/534 (34.64%), Postives = 287/534 (53.75%), Query Frame = 0

Query: 1   MSSRALQWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGF 60
           M  R  +W  LV  IW+Q+  GT+F+F +YSS  K  L +SQ++LN LA ASD GK FG+
Sbjct: 37  MFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGW 96

Query: 61  VSGFAANSLPLWLVLVIGSSLGLVGYGIQYLFIINQLHDPSYWVIFLLTVLAGNSICWIN 120
            SG A    PL +VL   +++G VGYG+Q+L I N +  P Y ++FL  +LAG SICW N
Sbjct: 97  SSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFN 156

Query: 121 TVCYIVAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLP 180
           T C+I+ IR+F  +R +A+ ++ S+ G+SA +Y+      F+  +   +  +LLLNS++P
Sbjct: 157 TACFILCIRHFPNNRALALSLTVSFNGISAALYS----LAFNAINPSSSNLYLLLNSLVP 216

Query: 181 LGVC------VFVSPLVRVHVVEEQGGMEIGVFLIF-MITIATGFFAVFTSLGSISTKLS 240
           L V       V   P +      +    +  VF I  ++ + T F  + +S  + S +L+
Sbjct: 217 LVVSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLN 276

Query: 241 PLTGLVGILVF-LLLPLAV------------------------AVAENVKRKTEQPEK-- 300
              G V +LVF L  PL V                         + E   +KT    K  
Sbjct: 277 -FIGAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTG 336

Query: 301 -------HRGGVEAAMEEIGAKEMLKRMNFWLHFGVYFFGATLGLVFLNNLGQIAESRRS 360
                    G      +E   + ++ R+ FWL++  YF G T+GLV+ NNLGQIA+S   
Sbjct: 337 YEHMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQ 396

Query: 361 SGVSSLVSLSSSFGFFGRLTPSLLDHFFPGKLMKSKPGWMVALLGAMCGGFFLLLWPT-- 420
           +  ++LV++ SSF FFGRL  +  D F   +   ++ GW    L      FFLL   +  
Sbjct: 397 NS-TTLVTIYSSFSFFGRLLSAAPD-FMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQ 456

Query: 421 DTSLCTSTAIIAGCTGAITSIAVSVTTELFGADNFSINHNLVVANIPLGSFLFGSLAGFL 480
            T+L T+TA+I   +G I + AVS+T++LFG ++  +NHN+++ NIP+GS L+G +A  +
Sbjct: 457 QTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGYIAASI 516

Query: 481 YR-------NQVPAGAGKCVGVECYRAVFLIWGCLCLVGTLLALVLFARTRSFY 485
           Y          + + +  C+G +CY   F+ WGCL ++G + +L L+ RT+  Y
Sbjct: 517 YEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562