CmaCh01G006540 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh01G006540
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Cytochrome P450
Location	Cma_Chr01: 3418591 .. 3421258 (+)
RNA-Seq Expression	CmaCh01G006540
Synteny	CmaCh01G006540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTAACGACGTCGTTTTGCCGTTCCCCTTCCCCTTCCCCTTCCCCTTCCCCTTCCCCTTCCCCTCTTTAATTCTCCTCATTTTCGCCTATTTGATTTGGTTTCATTTCCTCGCCCGTACGCTAACCGGTCCAAGAGTCTGGCCGGTCATAGGCAGCCTACCGGCTCTCATCTCGAATCGGCGGAGGTTCCATGACTGGATGGCTGGGAACCTCCGCTCCACAGGCAGCGCCGCCACATACCAAACTTGTACGGTGGCACTTCCATTCTTCGCTAGAAAACAAGGCTTCTACACGGTCACGTGCCACCCGAGAAACATCGAGCACGTGCTCCGAACCCGGTTCGAAAATTACCCGAAAGGACCGGACTGGCAAGCGGCTTTTCACGATCTTTTGGGTCAAGGGATTTTCAACAGCGACGGCGAGATTTGGCTGGTTCAGCGGAAGACGGCGGCGCTGGAGTTCACGACGAGGACGCTACGGCAGGCGATGGACCGGTGGGTGAATCGCACGATAAGGACGCGACTTTGGTGCATATTGGATAAGGCGGCGGAGTATAAAACGGCGGTTGATTTGCAGGATTTGTTGCTCCGATTGACTTTTGATAACATTTGTGGGCTCACTTTTGGGAAAGATCCTCAAACTTTGTCGCCGGAATTGCCTGCAACTCCCTTCGCTTTGGCGTTTGATTCCGCCACTGAAGCCACTCTTCACCGCCTTCTTTACCCTAGCATTTTATGGAGGTTTTATTTATTTTAACTTTTAATTACTAATTACTTTCCTTTTCATAATTATTAGTCTTTTAATTTTATTTAGCTCTTAAAATTAATTTTCTTAAAACAAATTATTTGGGTGATACCTAGATTTTTTTTTTAATAAAAAAATAAATATATTGTTTTGGTTTTTAATTAGTTTATTTTTGTTTTCTTGAACTAACTTTATAAGCTAAATGATATTATAAAAAAACATTGTTGCACATTATTAAAAGACTAAATTATTAAATTTTTAAAACTTAAATAATATCTTTAAACTTTTAAAATTAATATTAATACTTTTAAATTTTTTCTTAAATTTTTAAAACTAACATTAATATCTTTATTTTTTTTTATAAAAAAAATTATTTATGAACTTTATAAAAAAAAATTAAGGTTTTAATGAAAATTATTAAAAATATTTTATAAAAAAAATACTAGTGTTATTTATTCAAACTAATAAAAAAATCAATTTTAAGAATATTATTGAAAATTTTAAAATTTTAGAGATATTTTTTTATAGAAAAATACAAAATTTATAAATAGTCTTATTAAAAAAAAACTCCTATTTTTTTTTTATAAAAAAAGTTGCCATATTATTTTTGAATTAACTAATAAAATGTGACAAAATTTTCAAAAAATCAAATGAACCTAAAATAAACTATTTAAAAAGTTTAATGTAGTATAATAAATTTGAAAATAGCATTCAAACCTAGAATTTATTTAAGTTAATATAATAATGCATGTAAAAATCAATTAATAATAAATTTCTGACTCTTATTTGTTTATAATTAGGTTAAAGAAGCTTCTCTGCATCGGAATGGAAAGAAGGTTAAAGGAGAGTTTAAAAGTAATAGAGGAATACATAAACGACGCCGTTGCAGCCCGTAAAGAATCCCCCTCAGACGACTTATTATCCCGCTTCATGAAGAAGAAGCACGACGGGGACGGCGACGGCAACCGCTTCTCCGCCGCAGTGCTCCACCGCATCGCCTTAAACTTCGTCCTCGCCGGCCGGGACACATCCTCCGTGGCACTCACGTGGTTCTTTTGGCTCGTAATGAACCACCCGCACGTGGAGGCTACAATCCTCGCCGAAATCTCAACGGTTCTCCGTGAGACACGCGGCGACGATCCACGCCGGTGGATCGAAGAGCCGTTGATGTTCGACGAGGCCGATAAATTGGTGTATTTAAAAGCCGCGCTGGCCGAAACGCTGCGTTTATACCCGTCCGTACCACTGGATTTCAAATACGTCGTGGCTGATGACGTGTTGCCAGATGGCAGTTTTGTGCCAGCCGGTTCGACCGTGACGTATTCGATTTATTCGGTCGGGAGGATGAAGAGCATTTGGGGGGAGGATTGTGGGGAATTTAAACCAGACCGGTGGCTGTCGCCTGACGGGGACCGGTTCGAGGGGCCGAAAGATGGGTATAAATTCGTGGCGTTTAATGCTGGACCGAGAACTTGTTTGGGAAAGGATTTGGCTTATTTGCAAATGAAGTCCATCGCCTCCGCCGTGCTCCTCCGGTATCAGCTGGCGCCGGTTCCCGGTCACCGGGTGGAACAAAAAATGTCTCTTACTCTTTTTACGAAGAATGGGCTTCGGGTTTATTTGCATCCTCGCCGGCTCGGGTAGGAGGGGTGTTTTCGTCATTTCATTGTCACTTGAATTTGGCAGCTTTTGGCGAACGAATTATTTGTTTATTTTGGGTTTTGCATGGTACGAAAATTGGTTCTTTTCTTCTTTTATACTGCACAGGGTAAATTTGTCATTTCGTTGGTCTTCACGAGGGCATAATTGTCAATGTGTGTCTTTTTTTAATCTTTCTTTTTCTTTTTTATACGTAAATTGTTGGTGGTTCTTTTGGGGAGTGAAAGTGTTTGTAATTGTTGTATTTGGACGAATTGGTGGA

mRNA sequence

ATGACTAACGACGTCGTTTTGCCGTTCCCCTTCCCCTTCCCCTTCCCCTTCCCCTTCCCCTTCCCCTCTTTAATTCTCCTCATTTTCGCCTATTTGATTTGGTTTCATTTCCTCGCCCGTACGCTAACCGGTCCAAGAGTCTGGCCGGTCATAGGCAGCCTACCGGCTCTCATCTCGAATCGGCGGAGGTTCCATGACTGGATGGCTGGGAACCTCCGCTCCACAGGCAGCGCCGCCACATACCAAACTTGTACGGTGGCACTTCCATTCTTCGCTAGAAAACAAGGCTTCTACACGGTCACGTGCCACCCGAGAAACATCGAGCACGTGCTCCGAACCCGGTTCGAAAATTACCCGAAAGGACCGGACTGGCAAGCGGCTTTTCACGATCTTTTGGGTCAAGGGATTTTCAACAGCGACGGCGAGATTTGGCTGGTTCAGCGGAAGACGGCGGCGCTGGAGTTCACGACGAGGACGCTACGGCAGGCGATGGACCGGTGGGTGAATCGCACGATAAGGACGCGACTTTGGTGCATATTGGATAAGGCGGCGGAGTATAAAACGGCGGTTGATTTGCAGGATTTGTTGCTCCGATTGACTTTTGATAACATTTGTGGGCTCACTTTTGGGAAAGATCCTCAAACTTTGTCGCCGGAATTGCCTGCAACTCCCTTCGCTTTGGCGTTTGATTCCGCCACTGAAGCCACTCTTCACCGCCTTCTTTACCCTAGCATTTTATGGAGGTTAAAGAAGCTTCTCTGCATCGGAATGGAAAGAAGGTTAAAGGAGAGTTTAAAAGTAATAGAGGAATACATAAACGACGCCGTTGCAGCCCGTAAAGAATCCCCCTCAGACGACTTATTATCCCGCTTCATGAAGAAGAAGCACGACGGGGACGGCGACGGCAACCGCTTCTCCGCCGCAGTGCTCCACCGCATCGCCTTAAACTTCGTCCTCGCCGGCCGGGACACATCCTCCGTGGCACTCACGTGGTTCTTTTGGCTCGTAATGAACCACCCGCACGTGGAGGCTACAATCCTCGCCGAAATCTCAACGGTTCTCCGTGAGACACGCGGCGACGATCCACGCCGGTGGATCGAAGAGCCGTTGATGTTCGACGAGGCCGATAAATTGGTGTATTTAAAAGCCGCGCTGGCCGAAACGCTGCGTTTATACCCGTCCGTACCACTGGATTTCAAATACGTCGTGGCTGATGACGTGTTGCCAGATGGCAGTTTTGTGCCAGCCGGTTCGACCGTGACGTATTCGATTTATTCGGTCGGGAGGATGAAGAGCATTTGGGGGGAGGATTGTGGGGAATTTAAACCAGACCGGTGGCTGTCGCCTGACGGGGACCGGTTCGAGGGGCCGAAAGATGGGTATAAATTCGTGGCGTTTAATGCTGGACCGAGAACTTGTTTGGGAAAGGATTTGGCTTATTTGCAAATGAAGTCCATCGCCTCCGCCGTGCTCCTCCGGTATCAGCTGGCGCCGGTTCCCGGTCACCGGGTGGAACAAAAAATGTCTCTTACTCTTTTTACGAAGAATGGGCTTCGGGTTTATTTGCATCCTCGCCGGCTCGGGTAGGAGGGGTGTTTTCGTCATTTCATTGTCACTTGAATTTGGCAGCTTTTGGCGAACGAATTATTTGTTTATTTTGGGTTTTGCATGGTACGAAAATTGGTTCTTTTCTTCTTTTATACTGCACAGGGTAAATTTGTCATTTCGTTGGTCTTCACGAGGGCATAATTGTCAATGTGTGTCTTTTTTTAATCTTTCTTTTTCTTTTTTATACGTAAATTGTTGGTGGTTCTTTTGGGGAGTGAAAGTGTTTGTAATTGTTGTATTTGGACGAATTGGTGGA

Coding sequence (CDS)

ATGACTAACGACGTCGTTTTGCCGTTCCCCTTCCCCTTCCCCTTCCCCTTCCCCTTCCCCTTCCCCTCTTTAATTCTCCTCATTTTCGCCTATTTGATTTGGTTTCATTTCCTCGCCCGTACGCTAACCGGTCCAAGAGTCTGGCCGGTCATAGGCAGCCTACCGGCTCTCATCTCGAATCGGCGGAGGTTCCATGACTGGATGGCTGGGAACCTCCGCTCCACAGGCAGCGCCGCCACATACCAAACTTGTACGGTGGCACTTCCATTCTTCGCTAGAAAACAAGGCTTCTACACGGTCACGTGCCACCCGAGAAACATCGAGCACGTGCTCCGAACCCGGTTCGAAAATTACCCGAAAGGACCGGACTGGCAAGCGGCTTTTCACGATCTTTTGGGTCAAGGGATTTTCAACAGCGACGGCGAGATTTGGCTGGTTCAGCGGAAGACGGCGGCGCTGGAGTTCACGACGAGGACGCTACGGCAGGCGATGGACCGGTGGGTGAATCGCACGATAAGGACGCGACTTTGGTGCATATTGGATAAGGCGGCGGAGTATAAAACGGCGGTTGATTTGCAGGATTTGTTGCTCCGATTGACTTTTGATAACATTTGTGGGCTCACTTTTGGGAAAGATCCTCAAACTTTGTCGCCGGAATTGCCTGCAACTCCCTTCGCTTTGGCGTTTGATTCCGCCACTGAAGCCACTCTTCACCGCCTTCTTTACCCTAGCATTTTATGGAGGTTAAAGAAGCTTCTCTGCATCGGAATGGAAAGAAGGTTAAAGGAGAGTTTAAAAGTAATAGAGGAATACATAAACGACGCCGTTGCAGCCCGTAAAGAATCCCCCTCAGACGACTTATTATCCCGCTTCATGAAGAAGAAGCACGACGGGGACGGCGACGGCAACCGCTTCTCCGCCGCAGTGCTCCACCGCATCGCCTTAAACTTCGTCCTCGCCGGCCGGGACACATCCTCCGTGGCACTCACGTGGTTCTTTTGGCTCGTAATGAACCACCCGCACGTGGAGGCTACAATCCTCGCCGAAATCTCAACGGTTCTCCGTGAGACACGCGGCGACGATCCACGCCGGTGGATCGAAGAGCCGTTGATGTTCGACGAGGCCGATAAATTGGTGTATTTAAAAGCCGCGCTGGCCGAAACGCTGCGTTTATACCCGTCCGTACCACTGGATTTCAAATACGTCGTGGCTGATGACGTGTTGCCAGATGGCAGTTTTGTGCCAGCCGGTTCGACCGTGACGTATTCGATTTATTCGGTCGGGAGGATGAAGAGCATTTGGGGGGAGGATTGTGGGGAATTTAAACCAGACCGGTGGCTGTCGCCTGACGGGGACCGGTTCGAGGGGCCGAAAGATGGGTATAAATTCGTGGCGTTTAATGCTGGACCGAGAACTTGTTTGGGAAAGGATTTGGCTTATTTGCAAATGAAGTCCATCGCCTCCGCCGTGCTCCTCCGGTATCAGCTGGCGCCGGTTCCCGGTCACCGGGTGGAACAAAAAATGTCTCTTACTCTTTTTACGAAGAATGGGCTTCGGGTTTATTTGCATCCTCGCCGGCTCGGGTAG

Protein sequence

MTNDVVLPFPFPFPFPFPFPFPSLILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISNRRRFHDWMAGNLRSTGSAATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPKGPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCILDKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRLLYPSILWRLKKLLCIGMERRLKESLKVIEEYINDAVAARKESPSDDLLSRFMKKKHDGDGDGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGDDPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTVTYSIYSVGRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAYLQMKSIASAVLLRYQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRLG

Homology

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BLAST of CmaCh01G006540 vs. ExPASy Swiss-Prot
Match: P48422 (Cytochrome P450 86A1 OS=Arabidopsis thaliana OX=3702 GN=CYP86A1 PE=1 SV=2)

HSP 1 Score: 768.1 bits (1982), Expect = 6.6e-221
Identity = 368/501 (73.45%), Postives = 427/501 (85.23%), Query Frame = 0


Query: 25  ILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISNRRRFHDWMAGNLRSTGSAATYQTC 84
           +  +  Y +WF+FL+R LTGP+V P +GSLP LI+NR R HDW+A NLR+TG   TYQTC
Sbjct: 13  VAALSVYALWFYFLSRRLTGPKVLPFVGSLPYLIANRSRIHDWIADNLRATG--GTYQTC 72

Query: 85  TVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPKGPDWQAAFHDLLGQGIFNSDGEIW 144
           T+ +PF A+ QGFYTVTCHP+N+EH+L+TRF+NYPKGP W+AAFHDLLGQGIFNSDG+ W
Sbjct: 73  TMVIPFVAKAQGFYTVTCHPKNVEHILKTRFDNYPKGPMWRAAFHDLLGQGIFNSDGDTW 132

Query: 145 LVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCILDKAAEYKTAVDLQDLLLRLTFDNI 204
           L+QRKTAALEFTTRTLRQAM RWVN TI+ RLW ILD+A +    VDLQDL LRLTFDNI
Sbjct: 133 LMQRKTAALEFTTRTLRQAMARWVNGTIKNRLWLILDRAVQNNKPVDLQDLFLRLTFDNI 192

Query: 205 CGLTFGKDPQTLSPELPATPFALAFDSATEATLHRLLYPSILWRLKKLLCIGMERRLKES 264
           CGLTFGKDP+TLS +LP  PF++AFD+ATEATL RLLY   LWR++K + IG E +LK+S
Sbjct: 193 CGLTFGKDPETLSLDLPDNPFSVAFDTATEATLKRLLYTGFLWRIQKAMGIGSEDKLKKS 252

Query: 265 LKVIEEYINDAVAARKESPSDDLLSRFMKKKHDGDGDGNRFSAAVLHRIALNFVLAGRDT 324
           L+V+E Y+NDA+ ARK SPSDDLLSRF+KK+   D +GN     VL RIALNFVLAGRDT
Sbjct: 253 LEVVETYMNDAIDARKNSPSDDLLSRFLKKR---DVNGNVLPTDVLQRIALNFVLAGRDT 312

Query: 325 SSVALTWFFWLVMNHPHVEATILAEISTVLRETRGDDPRRWIEEPLMFDEADKLVYLKAA 384
           SSVAL+WFFWLVMN+  VE  I+ E+S VL+ETRG+D  +W EEPL FDEAD+LVYLKAA
Sbjct: 313 SSVALSWFFWLVMNNREVETKIVNELSMVLKETRGNDQEKWTEEPLEFDEADRLVYLKAA 372

Query: 385 LAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTVTYSIYSVGRMKSIWGEDCGEFKPD 444
           LAETLRLYPSVP DFKYVV DDVLPDG+FVP GSTVTYSIYS+GRMK+IWGEDC EF+P+
Sbjct: 373 LAETLRLYPSVPQDFKYVVDDDVLPDGTFVPRGSTVTYSIYSIGRMKTIWGEDCLEFRPE 432

Query: 445 RWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAYLQMKSIASAVLLRYQLAPVPGHRV 504
           RWL+ DG+RFE PKDGYKFVAFNAGPRTCLGKDLAY QMKS+ASAVLLRY++ PVPGHRV
Sbjct: 433 RWLTADGERFETPKDGYKFVAFNAGPRTCLGKDLAYNQMKSVASAVLLRYRVFPVPGHRV 492

Query: 505 EQKMSLTLFTKNGLRVYLHPR 526
           EQKMSLTLF KNGLRVYL PR
Sbjct: 493 EQKMSLTLFMKNGLRVYLQPR 508

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BLAST of CmaCh01G006540 vs. ExPASy Swiss-Prot
Match: B3RFJ6 (Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 9.6e-188
Identity = 323/516 (62.60%), Postives = 395/516 (76.55%), Query Frame = 0


Query: 24  LILLIFAYLIWFHFLARTLTGPR---VWPVIGSLPALISNRRRFHDWMAGNLRSTGSAAT 83
           ++ ++ AYL+WF  + +++ GP+   +WPV+GSLP LI N  R H+W+A NLR+   + T
Sbjct: 9   IVAIVAAYLLWFKSITKSMKGPKGPTMWPVVGSLPGLIENGNRMHEWIADNLRA--CSGT 68

Query: 84  YQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPKGPDWQAAFHDLLGQGIFNSD 143
           YQTC  A+PF ARKQ   TVTC P+N+EH+L+ RF+NYPKGP WQA FHDLLG+GIFNSD
Sbjct: 69  YQTCICAIPFLARKQ-LVTVTCDPKNLEHILKVRFDNYPKGPTWQAVFHDLLGEGIFNSD 128

Query: 144 GEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCILDKAAEYKTAVDLQDLLLRLT 203
           G+ WL QRKTAALEFTTRTLRQAM RWVNR I+ R   IL+ A      VDLQDLLLRLT
Sbjct: 129 GDTWLFQRKTAALEFTTRTLRQAMGRWVNRAIKNRFCPILEMAQVQGKPVDLQDLLLRLT 188

Query: 204 FDNICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRLLYPSILWRLKKLLCIGMERR 263
           FDNICGL FGKDP+TLSPELP   FA +FD ATEATLHR + P  +W+LKK+L +G+E  
Sbjct: 189 FDNICGLAFGKDPETLSPELPENNFATSFDRATEATLHRFIMPEFVWKLKKMLGLGLEVS 248

Query: 264 LKESLKVIEEYINDAVAARK---------ESPSDDLLSRFMKKKHDGDGDGNRFSAAVLH 323
           L  SLK ++ Y+ D +  RK             DDLLSRFMKKK         +S   L 
Sbjct: 249 LNNSLKQVDNYMTDVINTRKLELLNHQNGGPQHDDLLSRFMKKKES-------YSDKFLQ 308

Query: 324 RIALNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGDDPRRWIEEPLM 383
            +ALNF+LAGRDTSSVAL+WFFWLV ++P VE  IL EI T+L ETRG+D  +W+EEPL+
Sbjct: 309 HVALNFILAGRDTSSVALSWFFWLVSSNPRVEEKILVEICTILAETRGNDTSKWLEEPLV 368

Query: 384 FDEADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTVTYSIYSVGRMK 443
           F+E D+L+YLKAAL+ETLRLYPSVP D K+V++DD LPDG+FVPAGS +TYSIYS GRMK
Sbjct: 369 FEEVDQLMYLKAALSETLRLYPSVPEDSKHVISDDYLPDGTFVPAGSNITYSIYSTGRMK 428

Query: 444 SIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAYLQMKSIASAVL 503
            IWGEDC EFKP+RW+S DGD+F+  +D ++FVAFNAGPR CLGKDLAYLQMKSIA+AVL
Sbjct: 429 FIWGEDCLEFKPERWMSQDGDKFQ-VQDTFRFVAFNAGPRICLGKDLAYLQMKSIAAAVL 488

Query: 504 LRYQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRL 528
           LR++LA  PGH+VEQKMSLTLF K+GL + + PR L
Sbjct: 489 LRHRLAVAPGHKVEQKMSLTLFMKDGLVMNVTPRDL 513

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BLAST of CmaCh01G006540 vs. ExPASy Swiss-Prot
Match: O23066 (Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 5.8e-185
Identity = 326/514 (63.42%), Postives = 380/514 (73.93%), Query Frame = 0


Query: 24  LILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISNRRRFHDWMAGNLRSTGSAATYQT 83
           L+ ++ AY +WF  ++R L GPRVWPV+GSLP LI  R R HDW+  NLR+ G   TYQT
Sbjct: 9   LVAVVAAYWLWFQRISRWLKGPRVWPVLGSLPGLIEQRDRMHDWITENLRACG--GTYQT 68

Query: 84  CTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPKGPDWQAAFHDLLGQGIFNSDGEI 143
           C  A+PF A+KQG  TVTC P+NIEH+L+TRF+NYPKGP WQA FHD LGQGIFNSDG+ 
Sbjct: 69  CICAVPFLAKKQGLVTVTCDPKNIEHMLKTRFDNYPKGPTWQAVFHDFLGQGIFNSDGDT 128

Query: 144 WLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCILDKAAEYKTAVDLQDLLLRLTFDN 203
           WL QRKTAALEFTTRTLRQAM RWVNR I+ R   IL+ A      VDLQDL+LRLTFDN
Sbjct: 129 WLFQRKTAALEFTTRTLRQAMGRWVNRGIKLRFCPILETAQNNYEPVDLQDLILRLTFDN 188

Query: 204 ICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRLLYPSILWRLKKLLCIGMERRLKE 263
           ICGL FGKD +T +P LP   FA AFD ATEA+L R + P  LWRLKK L +G+E  L  
Sbjct: 189 ICGLAFGKDTRTCAPGLPENGFASAFDRATEASLQRFILPEFLWRLKKWLGLGLEVSLSR 248

Query: 264 SLKVIEEYINDAVAARKE----------SPSDDLLSRFMKKKHDGDGDGNRFSAAVLHRI 323
           SL  I+ Y++  +  RK+             DDLLSRFMKKK         +S   L  +
Sbjct: 249 SLGEIDGYLDAVINTRKQELLSQRESGVQRHDDLLSRFMKKKDQS------YSETFLRHV 308

Query: 324 ALNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGDDPRRWIEEPLMFD 383
           ALNF+LAGRDTSSVAL+WFFWL+  HP VE  I+ EI +VL ETRG D   W  EPL FD
Sbjct: 309 ALNFILAGRDTSSVALSWFFWLITTHPTVEDKIVREICSVLIETRGTDVSSWTAEPLEFD 368

Query: 384 EADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTVTYSIYSVGRMKSI 443
           E D+LVYLKAAL+ETLRLYPSVP D K+VV DD+LPDG+FVPAGS+VTYSIY+ GRMKS 
Sbjct: 369 EVDRLVYLKAALSETLRLYPSVPEDSKHVVNDDILPDGTFVPAGSSVTYSIYAAGRMKST 428

Query: 444 WGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAYLQMKSIASAVLLR 503
           WGEDC EFKP+RW+SPD  +F    D Y+FVAFNAGPR CLGKDLAYLQMK+IA+AVLLR
Sbjct: 429 WGEDCLEFKPERWISPDDGKFVN-HDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLLR 488

Query: 504 YQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRL 528
           ++L   PGH+VEQKMSLTLF KNGL V +H R L
Sbjct: 489 HRLTVAPGHKVEQKMSLTLFMKNGLLVNVHKRDL 513

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BLAST of CmaCh01G006540 vs. ExPASy Swiss-Prot
Match: O80823 (Cytochrome P450 86A8 OS=Arabidopsis thaliana OX=3702 GN=CYP86A8 PE=2 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 9.9e-185
Identity = 325/514 (63.23%), Postives = 383/514 (74.51%), Query Frame = 0


Query: 24  LILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISNRRRFHDWMAGNLRSTGSAATYQT 83
           ++  I AY +W  F++R L GPRVWP++GSLP LI N  R HDW++ NLR+   + TYQT
Sbjct: 9   ILSAITAYFLWLTFISRCLKGPRVWPILGSLPGLIENCERMHDWISDNLRA--CSGTYQT 68

Query: 84  CTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPKGPDWQAAFHDLLGQGIFNSDGEI 143
           C  A+PF A+KQG  TVTC PRN+EH+L+ RF+NYPKGP WQA FHDLLGQGIFNSDG+ 
Sbjct: 69  CICAIPFLAKKQGLVTVTCDPRNLEHILKNRFDNYPKGPTWQAVFHDLLGQGIFNSDGDT 128

Query: 144 WLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCILDKAAEYKTAVDLQDLLLRLTFDN 203
           WL QRKTAALEFTTRTLRQAM RWVNR I+ R   IL+ A      +DLQDLLLRLTFDN
Sbjct: 129 WLFQRKTAALEFTTRTLRQAMARWVNRAIKLRFLPILENARLGSEPIDLQDLLLRLTFDN 188

Query: 204 ICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRLLYPSILWRLKKLLCIGMERRLKE 263
           ICGLTFGKDP+T +P LP   FA+AFD ATEA+L R + P ILW+ K+ L +G+E  L  
Sbjct: 189 ICGLTFGKDPRTCAPGLPVNTFAVAFDRATEASLQRFILPEILWKFKRWLRLGLEVSLTR 248

Query: 264 SLKVIEEYINDAVAARKE---------SPSDDLLSRFMKKKHDGDGDGNRFSAAVLHRIA 323
           SL  ++ Y+++ +  RKE            DDLLSRF+KKK         +S   L R+A
Sbjct: 249 SLVQVDNYLSEIITTRKEEMMTQHNNGKHHDDLLSRFIKKKES-------YSDETLQRVA 308

Query: 324 LNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGDDPRRWIEEPLMFDE 383
           LNF+LAGRDTSSVAL+WFFWL+  HP +E  IL EI TVL ETRGDD   W +EPL  +E
Sbjct: 309 LNFILAGRDTSSVALSWFFWLITQHPAIEDKILREICTVLVETRGDDVALWTDEPLSCEE 368

Query: 384 ADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTVTYSIYSVGRMKSIW 443
            D+LV+LKAAL+ETLRLYPSVP D K  V DDVLPDG+FVPAGS++TYSIYS GRMKS W
Sbjct: 369 LDRLVFLKAALSETLRLYPSVPEDSKRAVKDDVLPDGTFVPAGSSITYSIYSAGRMKSTW 428

Query: 444 GEDCGEFKPDRWLS-PDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAYLQMKSIASAVLLR 503
           GEDC EFKP+RW+S  DG RF    D +KFVAFNAGPR CLGKDLAYLQMKSIASAVLLR
Sbjct: 429 GEDCLEFKPERWISQSDGGRFIN-HDPFKFVAFNAGPRICLGKDLAYLQMKSIASAVLLR 488

Query: 504 YQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRL 528
           ++L  V GH+VEQKMSLTLF K GL V +H R L
Sbjct: 489 HRLTVVTGHKVEQKMSLTLFMKYGLLVNVHERDL 512

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BLAST of CmaCh01G006540 vs. ExPASy Swiss-Prot
Match: Q9CAD6 (Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1)

HSP 1 Score: 635.2 bits (1637), Expect = 6.7e-181
Identity = 315/513 (61.40%), Postives = 383/513 (74.66%), Query Frame = 0


Query: 24  LILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISNRRRFHDWMAGNLRSTGSAATYQT 83
           ++ LI  Y+IWF  L R+  GPRVWP++GSLPALI+N  R HD++A NLR  G   TYQT
Sbjct: 9   ILTLIVTYIIWFVSLRRSYKGPRVWPLVGSLPALITNAHRMHDFIADNLRMCG--GTYQT 68

Query: 84  CTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPKGPDWQAAFHDLLGQGIFNSDGEI 143
           C   +PF A+KQG  TVTC P+N+EH+L+TRF+NYPKGP WQ+ FHDLLG GIFNSDG+ 
Sbjct: 69  CIFPIPFLAKKQGHVTVTCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLGDGIFNSDGDT 128

Query: 144 WLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCILDKAAEYKTAVDLQDLLLRLTFDN 203
           W  QRKTAALEFTTRTLRQAM RWV+R I+ RL  IL+ A      +DLQD+LLRLTFDN
Sbjct: 129 WRFQRKTAALEFTTRTLRQAMARWVDRAIKNRLVPILESARSRAEPIDLQDVLLRLTFDN 188

Query: 204 ICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRLLYPSILWRLKKLLCIGMERRLKE 263
           ICGLTFGKDP+TLSPE P   FA+AFD ATEATL R + P  +W+++K L +G+E  +  
Sbjct: 189 ICGLTFGKDPRTLSPEFPENGFAVAFDGATEATLQRFIMPEFIWKIRKWLRLGLEDDMSR 248

Query: 264 SLKVIEEYINDAVAARK---------ESPSDDLLSRFMKKKHDGDGDGNRFSAAVLHRIA 323
           S+  ++ Y+++ +  RK         ES  DDLLSRFMKKK         +S   L  +A
Sbjct: 249 SISHVDNYLSEIINTRKLELLGQQQDESRHDDLLSRFMKKKES-------YSDKYLKYVA 308

Query: 324 LNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGDDPRRWIEEPLMFDE 383
           LNF+LAGRDTSSVA++WFFWLV  +P VE  I+ EI T+L +TR  +  +W +EPL FDE
Sbjct: 309 LNFILAGRDTSSVAMSWFFWLVSLNPRVEEKIINEICTILIKTRDTNVSKWTDEPLTFDE 368

Query: 384 ADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTVTYSIYSVGRMKSIW 443
            D+LVYLKAAL+ETLRLYPSVP D K+VVA+DVLPDG+FVP+GS VTYSIYSVGRMK IW
Sbjct: 369 IDQLVYLKAALSETLRLYPSVPEDSKFVVANDVLPDGTFVPSGSNVTYSIYSVGRMKFIW 428

Query: 444 GEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAYLQMKSIASAVLLRY 503
           GEDC EFKP+RWL    D      + YKFVAFNAGPR CLGKDLAYLQMKSI +++LLR+
Sbjct: 429 GEDCLEFKPERWLEESRDE---KCNQYKFVAFNAGPRICLGKDLAYLQMKSITASILLRH 488

Query: 504 QLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRL 528
           +L   PGHRVEQKMSLTLF K GL++ +H R L
Sbjct: 489 RLTVAPGHRVEQKMSLTLFMKFGLKMDVHKRDL 509

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BLAST of CmaCh01G006540 vs. ExPASy TrEMBL
Match: A0A6J1K9B5 (cytochrome P450 86A1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492855 PE=3 SV=1)

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 528/528 (100.00%), Postives = 528/528 (100.00%), Query Frame = 0


Query: 1   MTNDVVLPFPFPFPFPFPFPFPSLILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN 60
           MTNDVVLPFPFPFPFPFPFPFPSLILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN
Sbjct: 1   MTNDVVLPFPFPFPFPFPFPFPSLILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN 60

Query: 61  RRRFHDWMAGNLRSTGSAATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK 120
           RRRFHDWMAGNLRSTGSAATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK
Sbjct: 61  RRRFHDWMAGNLRSTGSAATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK 120

Query: 121 GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL 180
           GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL
Sbjct: 121 GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL 180

Query: 181 DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRL 240
           DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRL
Sbjct: 181 DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRL 240

Query: 241 LYPSILWRLKKLLCIGMERRLKESLKVIEEYINDAVAARKESPSDDLLSRFMKKKHDGDG 300
           LYPSILWRLKKLLCIGMERRLKESLKVIEEYINDAVAARKESPSDDLLSRFMKKKHDGDG
Sbjct: 241 LYPSILWRLKKLLCIGMERRLKESLKVIEEYINDAVAARKESPSDDLLSRFMKKKHDGDG 300

Query: 301 DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGD 360
           DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGD
Sbjct: 301 DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGD 360

Query: 361 DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTV 420
           DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTV
Sbjct: 361 DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTV 420

Query: 421 TYSIYSVGRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY 480
           TYSIYSVGRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY
Sbjct: 421 TYSIYSVGRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY 480

Query: 481 LQMKSIASAVLLRYQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRLG 529
           LQMKSIASAVLLRYQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRLG
Sbjct: 481 LQMKSIASAVLLRYQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRLG 528

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BLAST of CmaCh01G006540 vs. ExPASy TrEMBL
Match: A0A6J1K6Z0 (cytochrome P450 86A1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492855 PE=3 SV=1)

HSP 1 Score: 1054.7 bits (2726), Expect = 1.3e-304
Identity = 518/528 (98.11%), Postives = 518/528 (98.11%), Query Frame = 0


Query: 1   MTNDVVLPFPFPFPFPFPFPFPSLILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN 60
           MTNDVVL          PFPFPSLILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN
Sbjct: 1   MTNDVVL----------PFPFPSLILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN 60

Query: 61  RRRFHDWMAGNLRSTGSAATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK 120
           RRRFHDWMAGNLRSTGSAATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK
Sbjct: 61  RRRFHDWMAGNLRSTGSAATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK 120

Query: 121 GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL 180
           GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL
Sbjct: 121 GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL 180

Query: 181 DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRL 240
           DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRL
Sbjct: 181 DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRL 240

Query: 241 LYPSILWRLKKLLCIGMERRLKESLKVIEEYINDAVAARKESPSDDLLSRFMKKKHDGDG 300
           LYPSILWRLKKLLCIGMERRLKESLKVIEEYINDAVAARKESPSDDLLSRFMKKKHDGDG
Sbjct: 241 LYPSILWRLKKLLCIGMERRLKESLKVIEEYINDAVAARKESPSDDLLSRFMKKKHDGDG 300

Query: 301 DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGD 360
           DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGD
Sbjct: 301 DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGD 360

Query: 361 DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTV 420
           DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTV
Sbjct: 361 DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTV 420

Query: 421 TYSIYSVGRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY 480
           TYSIYSVGRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY
Sbjct: 421 TYSIYSVGRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY 480

Query: 481 LQMKSIASAVLLRYQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRLG 529
           LQMKSIASAVLLRYQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRLG
Sbjct: 481 LQMKSIASAVLLRYQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRLG 518

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BLAST of CmaCh01G006540 vs. ExPASy TrEMBL
Match: A0A6J1GB79 (cytochrome P450 86A1-like OS=Cucurbita moschata OX=3662 GN=LOC111452582 PE=3 SV=1)

HSP 1 Score: 1025.8 bits (2651), Expect = 6.5e-296
Identity = 503/528 (95.27%), Postives = 508/528 (96.21%), Query Frame = 0


Query: 1   MTNDVVLPFPFPFPFPFPFPFPSLILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN 60
           M NDVVLPFPFP          SL+LLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN
Sbjct: 1   MANDVVLPFPFP---------SSLLLLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN 60

Query: 61  RRRFHDWMAGNLRSTGSAATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK 120
           RR  HDWMAGNLRSTG AATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK
Sbjct: 61  RRSLHDWMAGNLRSTGGAATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK 120

Query: 121 GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL 180
           GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL
Sbjct: 121 GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL 180

Query: 181 DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRL 240
           DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPA+PFALAFDSATEATLHRL
Sbjct: 181 DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPASPFALAFDSATEATLHRL 240

Query: 241 LYPSILWRLKKLLCIGMERRLKESLKVIEEYINDAVAARKESPSDDLLSRFMKKKHDGDG 300
           LYP ILWRLKKLL IGMERRLKESLKVIEEYINDAVA+RKESPSDDLLSRFMKKKHDGDG
Sbjct: 241 LYPCILWRLKKLLGIGMERRLKESLKVIEEYINDAVASRKESPSDDLLSRFMKKKHDGDG 300

Query: 301 DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGD 360
           DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEA ILAEISTVLR+TRGD
Sbjct: 301 DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEAKILAEISTVLRQTRGD 360

Query: 361 DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTV 420
           DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVP DFKYVVADDVLPDGSFVPAGSTV
Sbjct: 361 DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVPQDFKYVVADDVLPDGSFVPAGSTV 420

Query: 421 TYSIYSVGRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY 480
           TYSIYS GRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY
Sbjct: 421 TYSIYSAGRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY 480

Query: 481 LQMKSIASAVLLRYQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRLG 529
           LQMKS+ASAVLLRYQLAPVPGHRVEQKMSLTLF KNGLRVYLHPRRLG
Sbjct: 481 LQMKSVASAVLLRYQLAPVPGHRVEQKMSLTLFMKNGLRVYLHPRRLG 519

[Close]

BLAST of CmaCh01G006540 vs. ExPASy TrEMBL
Match: A0A0A0LV45 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G528600 PE=3 SV=1)

HSP 1 Score: 933.3 bits (2411), Expect = 4.4e-268
Identity = 459/529 (86.77%), Postives = 483/529 (91.30%), Query Frame = 0


Query: 1   MTNDVVLPFPFPFPFPFPFPFPSLILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN 60
           M ND V PF            P  + LI  Y IWFHFLAR LTGP VWP+IGSLPALISN
Sbjct: 1   MANDDVFPF-----------LPLFLFLILTYFIWFHFLARKLTGPTVWPLIGSLPALISN 60

Query: 61  RRRFHDWMAGNLRSTGSAATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK 120
           RR  HDW+AGNLR+TG AATYQTCTVALPF A+KQGFYTVTCHPRNIEHVLRTRFENYPK
Sbjct: 61  RRSLHDWIAGNLRATGGAATYQTCTVALPFIAKKQGFYTVTCHPRNIEHVLRTRFENYPK 120

Query: 121 GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL 180
           GPDWQAAFHDLLGQGIFNSDGEIWL+QRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL
Sbjct: 121 GPDWQAAFHDLLGQGIFNSDGEIWLIQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL 180

Query: 181 DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRL 240
           +KAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPA PFALAFD+ATEATL RL
Sbjct: 181 EKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPANPFALAFDTATEATLQRL 240

Query: 241 LYPSILWRLKKLLCIGMERRLKESLKVIEEYINDAVAAR-KESPSDDLLSRFMKKKHDGD 300
           LYP +LWRL+K+L IGMERRL++SLKV+EEYINDAVAAR KESPSDDLLSRFMKK+    
Sbjct: 241 LYPGLLWRLEKVLGIGMERRLQKSLKVVEEYINDAVAARNKESPSDDLLSRFMKKR---- 300

Query: 301 GDGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRG 360
            D +RFS+AVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVE  IL+EISTVLR+TRG
Sbjct: 301 -DDDRFSSAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEEKILSEISTVLRQTRG 360

Query: 361 DDPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGST 420
           DD RRWIEEPL+FDEADKLVYLKAALAETLRLYPSVP DFKYVVADDVLPDG+FVPAGST
Sbjct: 361 DDIRRWIEEPLVFDEADKLVYLKAALAETLRLYPSVPEDFKYVVADDVLPDGTFVPAGST 420

Query: 421 VTYSIYSVGRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLA 480
           VTYSIYSVGRMKSIWGEDC EFKPDRWLSP GDRFEGPKD YKFVAFNAGPRTCLGKDLA
Sbjct: 421 VTYSIYSVGRMKSIWGEDCTEFKPDRWLSPTGDRFEGPKDAYKFVAFNAGPRTCLGKDLA 480

Query: 481 YLQMKSIASAVLLRYQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRLG 529
           YLQMKS+ASAVLLRY+L+PVPGHRVEQKMSLTLF KNGLRVYLHPRRLG
Sbjct: 481 YLQMKSVASAVLLRYRLSPVPGHRVEQKMSLTLFMKNGLRVYLHPRRLG 513

[Close]

BLAST of CmaCh01G006540 vs. ExPASy TrEMBL
Match: A0A6J1HZR9 (cytochrome P450 86A1-like OS=Cucurbita maxima OX=3661 GN=LOC111469581 PE=3 SV=1)

HSP 1 Score: 931.8 bits (2407), Expect = 1.3e-267
Identity = 458/528 (86.74%), Postives = 479/528 (90.72%), Query Frame = 0


Query: 1   MTNDVVLPFPFPFPFPFPFPFPSLILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN 60
           M NDVV PFP  F           + L   YLIWF+FLAR LTGPRVWPVIGSLPAL SN
Sbjct: 1   MANDVVFPFPTFF-----------LTLFLTYLIWFYFLARKLTGPRVWPVIGSLPALFSN 60

Query: 61  RRRFHDWMAGNLRSTGSAATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK 120
           RRR HDWMAGNLR TG+AATYQTCTVA+PF A+KQGFYTVTCHPRNIEHVLRTRFENYPK
Sbjct: 61  RRRLHDWMAGNLRDTGAAATYQTCTVAVPFIAKKQGFYTVTCHPRNIEHVLRTRFENYPK 120

Query: 121 GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL 180
           GPDWQAAFHDLLGQGIFNSDGEIWL+QRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL
Sbjct: 121 GPDWQAAFHDLLGQGIFNSDGEIWLIQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL 180

Query: 181 DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRL 240
           DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDP+TLSPELP   FALAFD+ATEATL RL
Sbjct: 181 DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPETLSPELPTNSFALAFDTATEATLQRL 240

Query: 241 LYPSILWRLKKLLCIGMERRLKESLKVIEEYINDAVAARKESPSDDLLSRFMKKKHDGDG 300
           LYP ++WR +KLL IGMERRLK+ LKV+EEYINDAVA+RKESPSDDLLSRFMKK+     
Sbjct: 241 LYPGLIWRFEKLLGIGMERRLKKCLKVVEEYINDAVASRKESPSDDLLSRFMKKR----- 300

Query: 301 DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGD 360
           D +RFSA VLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVE  IL EISTVLR+TRGD
Sbjct: 301 DDDRFSATVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEEKILTEISTVLRQTRGD 360

Query: 361 DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTV 420
           D RRWIEEPL+FDEADKLVYLKAALAETLRLYPSVP DFKYVVADDVLPDG+FVPAGSTV
Sbjct: 361 DTRRWIEEPLVFDEADKLVYLKAALAETLRLYPSVPQDFKYVVADDVLPDGTFVPAGSTV 420

Query: 421 TYSIYSVGRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY 480
           TYSIYSVGRMKSIWGEDC EFKPDRWLSP GDRFEG KD YKFVAFNAGPRTCLGKDLAY
Sbjct: 421 TYSIYSVGRMKSIWGEDCEEFKPDRWLSPAGDRFEGQKDVYKFVAFNAGPRTCLGKDLAY 480

Query: 481 LQMKSIASAVLLRYQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRLG 529
           LQMKS+ASAVLLRY+LAPVPGHRVEQKMSLTLF KNGLRVYLHPRRLG
Sbjct: 481 LQMKSVASAVLLRYRLAPVPGHRVEQKMSLTLFMKNGLRVYLHPRRLG 512

[Close]

BLAST of CmaCh01G006540 vs. NCBI nr
Match: XP_022998106.1 (cytochrome P450 86A1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 528/528 (100.00%), Postives = 528/528 (100.00%), Query Frame = 0


Query: 1   MTNDVVLPFPFPFPFPFPFPFPSLILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN 60
           MTNDVVLPFPFPFPFPFPFPFPSLILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN
Sbjct: 1   MTNDVVLPFPFPFPFPFPFPFPSLILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN 60

Query: 61  RRRFHDWMAGNLRSTGSAATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK 120
           RRRFHDWMAGNLRSTGSAATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK
Sbjct: 61  RRRFHDWMAGNLRSTGSAATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK 120

Query: 121 GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL 180
           GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL
Sbjct: 121 GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL 180

Query: 181 DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRL 240
           DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRL
Sbjct: 181 DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRL 240

Query: 241 LYPSILWRLKKLLCIGMERRLKESLKVIEEYINDAVAARKESPSDDLLSRFMKKKHDGDG 300
           LYPSILWRLKKLLCIGMERRLKESLKVIEEYINDAVAARKESPSDDLLSRFMKKKHDGDG
Sbjct: 241 LYPSILWRLKKLLCIGMERRLKESLKVIEEYINDAVAARKESPSDDLLSRFMKKKHDGDG 300

Query: 301 DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGD 360
           DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGD
Sbjct: 301 DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGD 360

Query: 361 DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTV 420
           DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTV
Sbjct: 361 DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTV 420

Query: 421 TYSIYSVGRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY 480
           TYSIYSVGRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY
Sbjct: 421 TYSIYSVGRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY 480

Query: 481 LQMKSIASAVLLRYQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRLG 529
           LQMKSIASAVLLRYQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRLG
Sbjct: 481 LQMKSIASAVLLRYQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRLG 528

[Close]

BLAST of CmaCh01G006540 vs. NCBI nr
Match: XP_022998107.1 (cytochrome P450 86A1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1054.7 bits (2726), Expect = 2.7e-304
Identity = 518/528 (98.11%), Postives = 518/528 (98.11%), Query Frame = 0


Query: 1   MTNDVVLPFPFPFPFPFPFPFPSLILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN 60
           MTNDVVL          PFPFPSLILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN
Sbjct: 1   MTNDVVL----------PFPFPSLILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN 60

Query: 61  RRRFHDWMAGNLRSTGSAATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK 120
           RRRFHDWMAGNLRSTGSAATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK
Sbjct: 61  RRRFHDWMAGNLRSTGSAATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK 120

Query: 121 GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL 180
           GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL
Sbjct: 121 GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL 180

Query: 181 DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRL 240
           DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRL
Sbjct: 181 DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRL 240

Query: 241 LYPSILWRLKKLLCIGMERRLKESLKVIEEYINDAVAARKESPSDDLLSRFMKKKHDGDG 300
           LYPSILWRLKKLLCIGMERRLKESLKVIEEYINDAVAARKESPSDDLLSRFMKKKHDGDG
Sbjct: 241 LYPSILWRLKKLLCIGMERRLKESLKVIEEYINDAVAARKESPSDDLLSRFMKKKHDGDG 300

Query: 301 DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGD 360
           DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGD
Sbjct: 301 DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGD 360

Query: 361 DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTV 420
           DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTV
Sbjct: 361 DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTV 420

Query: 421 TYSIYSVGRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY 480
           TYSIYSVGRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY
Sbjct: 421 TYSIYSVGRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY 480

Query: 481 LQMKSIASAVLLRYQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRLG 529
           LQMKSIASAVLLRYQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRLG
Sbjct: 481 LQMKSIASAVLLRYQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRLG 518

[Close]

BLAST of CmaCh01G006540 vs. NCBI nr
Match: KAG7036979.1 (Cytochrome P450 86A1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1038.9 bits (2685), Expect = 1.5e-299
Identity = 509/528 (96.40%), Postives = 513/528 (97.16%), Query Frame = 0


Query: 1   MTNDVVLPFPFPFPFPFPFPFPSLILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN 60
           M NDVVL      PFPFPFPFPSLILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN
Sbjct: 1   MANDVVL------PFPFPFPFPSLILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN 60

Query: 61  RRRFHDWMAGNLRSTGSAATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK 120
           RR  HDWMAGNLRSTG AATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK
Sbjct: 61  RRSLHDWMAGNLRSTGGAATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK 120

Query: 121 GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL 180
           GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL
Sbjct: 121 GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL 180

Query: 181 DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRL 240
           DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPA+PFALAFDSATEATLHRL
Sbjct: 181 DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPASPFALAFDSATEATLHRL 240

Query: 241 LYPSILWRLKKLLCIGMERRLKESLKVIEEYINDAVAARKESPSDDLLSRFMKKKHDGDG 300
           LYPSILWRLKKLL IGMERRLKESLKVIEEYINDAVA+RKESPSDDLLSRFMKKKHDGDG
Sbjct: 241 LYPSILWRLKKLLGIGMERRLKESLKVIEEYINDAVASRKESPSDDLLSRFMKKKHDGDG 300

Query: 301 DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGD 360
           DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEA ILAEISTVLR+TRGD
Sbjct: 301 DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEAKILAEISTVLRQTRGD 360

Query: 361 DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTV 420
           DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVP DFKYVVADDVLPDGSFVPAGSTV
Sbjct: 361 DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVPQDFKYVVADDVLPDGSFVPAGSTV 420

Query: 421 TYSIYSVGRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY 480
           TYSIYS GRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY
Sbjct: 421 TYSIYSAGRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY 480

Query: 481 LQMKSIASAVLLRYQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRLG 529
           LQMKS+ASAVLLRYQLAPVPGHRVEQKMSLTLF KNGLRVYLHPRRLG
Sbjct: 481 LQMKSVASAVLLRYQLAPVPGHRVEQKMSLTLFMKNGLRVYLHPRRLG 522

[Close]

BLAST of CmaCh01G006540 vs. NCBI nr
Match: KAG6607300.1 (Cytochrome P450 86A1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1029.2 bits (2660), Expect = 1.2e-296
Identity = 505/528 (95.64%), Postives = 509/528 (96.40%), Query Frame = 0


Query: 1   MTNDVVLPFPFPFPFPFPFPFPSLILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN 60
           M NDVVL          PFPFPSLILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN
Sbjct: 1   MANDVVL----------PFPFPSLILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN 60

Query: 61  RRRFHDWMAGNLRSTGSAATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK 120
           RR  HDWMAGNLRSTG AATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK
Sbjct: 61  RRSLHDWMAGNLRSTGGAATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK 120

Query: 121 GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL 180
           GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL
Sbjct: 121 GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL 180

Query: 181 DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRL 240
           DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPA+PFALAFDSATEATLHRL
Sbjct: 181 DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPASPFALAFDSATEATLHRL 240

Query: 241 LYPSILWRLKKLLCIGMERRLKESLKVIEEYINDAVAARKESPSDDLLSRFMKKKHDGDG 300
           LYPSILWRLKKLL IGMERRLKESLKVIEEYINDAVA+RKESPSDDLLSRFMKKKHDGDG
Sbjct: 241 LYPSILWRLKKLLGIGMERRLKESLKVIEEYINDAVASRKESPSDDLLSRFMKKKHDGDG 300

Query: 301 DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGD 360
           DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEA ILAEISTVLR+TRGD
Sbjct: 301 DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEAKILAEISTVLRQTRGD 360

Query: 361 DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTV 420
           DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVP DFKYVVADDVLPDGSFVPAGSTV
Sbjct: 361 DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVPQDFKYVVADDVLPDGSFVPAGSTV 420

Query: 421 TYSIYSVGRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY 480
           TYSIYS GRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY
Sbjct: 421 TYSIYSAGRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY 480

Query: 481 LQMKSIASAVLLRYQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRLG 529
           LQMKS+ASAVLLRYQLAPVPGHRVEQKMSLTLF KNGLRVYLHPRRLG
Sbjct: 481 LQMKSVASAVLLRYQLAPVPGHRVEQKMSLTLFMKNGLRVYLHPRRLG 518

[Close]

BLAST of CmaCh01G006540 vs. NCBI nr
Match: XP_022949142.1 (cytochrome P450 86A1-like [Cucurbita moschata])

HSP 1 Score: 1025.8 bits (2651), Expect = 1.3e-295
Identity = 503/528 (95.27%), Postives = 508/528 (96.21%), Query Frame = 0


Query: 1   MTNDVVLPFPFPFPFPFPFPFPSLILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN 60
           M NDVVLPFPFP          SL+LLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN
Sbjct: 1   MANDVVLPFPFP---------SSLLLLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISN 60

Query: 61  RRRFHDWMAGNLRSTGSAATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK 120
           RR  HDWMAGNLRSTG AATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK
Sbjct: 61  RRSLHDWMAGNLRSTGGAATYQTCTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPK 120

Query: 121 GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL 180
           GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL
Sbjct: 121 GPDWQAAFHDLLGQGIFNSDGEIWLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCIL 180

Query: 181 DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRL 240
           DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPA+PFALAFDSATEATLHRL
Sbjct: 181 DKAAEYKTAVDLQDLLLRLTFDNICGLTFGKDPQTLSPELPASPFALAFDSATEATLHRL 240

Query: 241 LYPSILWRLKKLLCIGMERRLKESLKVIEEYINDAVAARKESPSDDLLSRFMKKKHDGDG 300
           LYP ILWRLKKLL IGMERRLKESLKVIEEYINDAVA+RKESPSDDLLSRFMKKKHDGDG
Sbjct: 241 LYPCILWRLKKLLGIGMERRLKESLKVIEEYINDAVASRKESPSDDLLSRFMKKKHDGDG 300

Query: 301 DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGD 360
           DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEA ILAEISTVLR+TRGD
Sbjct: 301 DGNRFSAAVLHRIALNFVLAGRDTSSVALTWFFWLVMNHPHVEAKILAEISTVLRQTRGD 360

Query: 361 DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTV 420
           DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVP DFKYVVADDVLPDGSFVPAGSTV
Sbjct: 361 DPRRWIEEPLMFDEADKLVYLKAALAETLRLYPSVPQDFKYVVADDVLPDGSFVPAGSTV 420

Query: 421 TYSIYSVGRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY 480
           TYSIYS GRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY
Sbjct: 421 TYSIYSAGRMKSIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAY 480

Query: 481 LQMKSIASAVLLRYQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRLG 529
           LQMKS+ASAVLLRYQLAPVPGHRVEQKMSLTLF KNGLRVYLHPRRLG
Sbjct: 481 LQMKSVASAVLLRYQLAPVPGHRVEQKMSLTLFMKNGLRVYLHPRRLG 519

[Close]

BLAST of CmaCh01G006540 vs. TAIR 10
Match: AT5G58860.1 (cytochrome P450, family 86, subfamily A, polypeptide 1 )

HSP 1 Score: 768.1 bits (1982), Expect = 4.7e-222
Identity = 368/501 (73.45%), Postives = 427/501 (85.23%), Query Frame = 0


Query: 25  ILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISNRRRFHDWMAGNLRSTGSAATYQTC 84
           +  +  Y +WF+FL+R LTGP+V P +GSLP LI+NR R HDW+A NLR+TG   TYQTC
Sbjct: 13  VAALSVYALWFYFLSRRLTGPKVLPFVGSLPYLIANRSRIHDWIADNLRATG--GTYQTC 72

Query: 85  TVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPKGPDWQAAFHDLLGQGIFNSDGEIW 144
           T+ +PF A+ QGFYTVTCHP+N+EH+L+TRF+NYPKGP W+AAFHDLLGQGIFNSDG+ W
Sbjct: 73  TMVIPFVAKAQGFYTVTCHPKNVEHILKTRFDNYPKGPMWRAAFHDLLGQGIFNSDGDTW 132

Query: 145 LVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCILDKAAEYKTAVDLQDLLLRLTFDNI 204
           L+QRKTAALEFTTRTLRQAM RWVN TI+ RLW ILD+A +    VDLQDL LRLTFDNI
Sbjct: 133 LMQRKTAALEFTTRTLRQAMARWVNGTIKNRLWLILDRAVQNNKPVDLQDLFLRLTFDNI 192

Query: 205 CGLTFGKDPQTLSPELPATPFALAFDSATEATLHRLLYPSILWRLKKLLCIGMERRLKES 264
           CGLTFGKDP+TLS +LP  PF++AFD+ATEATL RLLY   LWR++K + IG E +LK+S
Sbjct: 193 CGLTFGKDPETLSLDLPDNPFSVAFDTATEATLKRLLYTGFLWRIQKAMGIGSEDKLKKS 252

Query: 265 LKVIEEYINDAVAARKESPSDDLLSRFMKKKHDGDGDGNRFSAAVLHRIALNFVLAGRDT 324
           L+V+E Y+NDA+ ARK SPSDDLLSRF+KK+   D +GN     VL RIALNFVLAGRDT
Sbjct: 253 LEVVETYMNDAIDARKNSPSDDLLSRFLKKR---DVNGNVLPTDVLQRIALNFVLAGRDT 312

Query: 325 SSVALTWFFWLVMNHPHVEATILAEISTVLRETRGDDPRRWIEEPLMFDEADKLVYLKAA 384
           SSVAL+WFFWLVMN+  VE  I+ E+S VL+ETRG+D  +W EEPL FDEAD+LVYLKAA
Sbjct: 313 SSVALSWFFWLVMNNREVETKIVNELSMVLKETRGNDQEKWTEEPLEFDEADRLVYLKAA 372

Query: 385 LAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTVTYSIYSVGRMKSIWGEDCGEFKPD 444
           LAETLRLYPSVP DFKYVV DDVLPDG+FVP GSTVTYSIYS+GRMK+IWGEDC EF+P+
Sbjct: 373 LAETLRLYPSVPQDFKYVVDDDVLPDGTFVPRGSTVTYSIYSIGRMKTIWGEDCLEFRPE 432

Query: 445 RWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAYLQMKSIASAVLLRYQLAPVPGHRV 504
           RWL+ DG+RFE PKDGYKFVAFNAGPRTCLGKDLAY QMKS+ASAVLLRY++ PVPGHRV
Sbjct: 433 RWLTADGERFETPKDGYKFVAFNAGPRTCLGKDLAYNQMKSVASAVLLRYRVFPVPGHRV 492

Query: 505 EQKMSLTLFTKNGLRVYLHPR 526
           EQKMSLTLF KNGLRVYL PR
Sbjct: 493 EQKMSLTLFMKNGLRVYLQPR 508

[Close]

BLAST of CmaCh01G006540 vs. TAIR 10
Match: AT4G00360.1 (cytochrome P450, family 86, subfamily A, polypeptide 2 )

HSP 1 Score: 648.7 bits (1672), Expect = 4.1e-186
Identity = 326/514 (63.42%), Postives = 380/514 (73.93%), Query Frame = 0


Query: 24  LILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISNRRRFHDWMAGNLRSTGSAATYQT 83
           L+ ++ AY +WF  ++R L GPRVWPV+GSLP LI  R R HDW+  NLR+ G   TYQT
Sbjct: 9   LVAVVAAYWLWFQRISRWLKGPRVWPVLGSLPGLIEQRDRMHDWITENLRACG--GTYQT 68

Query: 84  CTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPKGPDWQAAFHDLLGQGIFNSDGEI 143
           C  A+PF A+KQG  TVTC P+NIEH+L+TRF+NYPKGP WQA FHD LGQGIFNSDG+ 
Sbjct: 69  CICAVPFLAKKQGLVTVTCDPKNIEHMLKTRFDNYPKGPTWQAVFHDFLGQGIFNSDGDT 128

Query: 144 WLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCILDKAAEYKTAVDLQDLLLRLTFDN 203
           WL QRKTAALEFTTRTLRQAM RWVNR I+ R   IL+ A      VDLQDL+LRLTFDN
Sbjct: 129 WLFQRKTAALEFTTRTLRQAMGRWVNRGIKLRFCPILETAQNNYEPVDLQDLILRLTFDN 188

Query: 204 ICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRLLYPSILWRLKKLLCIGMERRLKE 263
           ICGL FGKD +T +P LP   FA AFD ATEA+L R + P  LWRLKK L +G+E  L  
Sbjct: 189 ICGLAFGKDTRTCAPGLPENGFASAFDRATEASLQRFILPEFLWRLKKWLGLGLEVSLSR 248

Query: 264 SLKVIEEYINDAVAARKE----------SPSDDLLSRFMKKKHDGDGDGNRFSAAVLHRI 323
           SL  I+ Y++  +  RK+             DDLLSRFMKKK         +S   L  +
Sbjct: 249 SLGEIDGYLDAVINTRKQELLSQRESGVQRHDDLLSRFMKKKDQS------YSETFLRHV 308

Query: 324 ALNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGDDPRRWIEEPLMFD 383
           ALNF+LAGRDTSSVAL+WFFWL+  HP VE  I+ EI +VL ETRG D   W  EPL FD
Sbjct: 309 ALNFILAGRDTSSVALSWFFWLITTHPTVEDKIVREICSVLIETRGTDVSSWTAEPLEFD 368

Query: 384 EADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTVTYSIYSVGRMKSI 443
           E D+LVYLKAAL+ETLRLYPSVP D K+VV DD+LPDG+FVPAGS+VTYSIY+ GRMKS 
Sbjct: 369 EVDRLVYLKAALSETLRLYPSVPEDSKHVVNDDILPDGTFVPAGSSVTYSIYAAGRMKST 428

Query: 444 WGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAYLQMKSIASAVLLR 503
           WGEDC EFKP+RW+SPD  +F    D Y+FVAFNAGPR CLGKDLAYLQMK+IA+AVLLR
Sbjct: 429 WGEDCLEFKPERWISPDDGKFVN-HDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLLR 488

Query: 504 YQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRL 528
           ++L   PGH+VEQKMSLTLF KNGL V +H R L
Sbjct: 489 HRLTVAPGHKVEQKMSLTLFMKNGLLVNVHKRDL 513

[Close]

BLAST of CmaCh01G006540 vs. TAIR 10
Match: AT2G45970.1 (cytochrome P450, family 86, subfamily A, polypeptide 8 )

HSP 1 Score: 647.9 bits (1670), Expect = 7.1e-186
Identity = 325/514 (63.23%), Postives = 383/514 (74.51%), Query Frame = 0


Query: 24  LILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISNRRRFHDWMAGNLRSTGSAATYQT 83
           ++  I AY +W  F++R L GPRVWP++GSLP LI N  R HDW++ NLR+   + TYQT
Sbjct: 9   ILSAITAYFLWLTFISRCLKGPRVWPILGSLPGLIENCERMHDWISDNLRA--CSGTYQT 68

Query: 84  CTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPKGPDWQAAFHDLLGQGIFNSDGEI 143
           C  A+PF A+KQG  TVTC PRN+EH+L+ RF+NYPKGP WQA FHDLLGQGIFNSDG+ 
Sbjct: 69  CICAIPFLAKKQGLVTVTCDPRNLEHILKNRFDNYPKGPTWQAVFHDLLGQGIFNSDGDT 128

Query: 144 WLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCILDKAAEYKTAVDLQDLLLRLTFDN 203
           WL QRKTAALEFTTRTLRQAM RWVNR I+ R   IL+ A      +DLQDLLLRLTFDN
Sbjct: 129 WLFQRKTAALEFTTRTLRQAMARWVNRAIKLRFLPILENARLGSEPIDLQDLLLRLTFDN 188

Query: 204 ICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRLLYPSILWRLKKLLCIGMERRLKE 263
           ICGLTFGKDP+T +P LP   FA+AFD ATEA+L R + P ILW+ K+ L +G+E  L  
Sbjct: 189 ICGLTFGKDPRTCAPGLPVNTFAVAFDRATEASLQRFILPEILWKFKRWLRLGLEVSLTR 248

Query: 264 SLKVIEEYINDAVAARKE---------SPSDDLLSRFMKKKHDGDGDGNRFSAAVLHRIA 323
           SL  ++ Y+++ +  RKE            DDLLSRF+KKK         +S   L R+A
Sbjct: 249 SLVQVDNYLSEIITTRKEEMMTQHNNGKHHDDLLSRFIKKKES-------YSDETLQRVA 308

Query: 324 LNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGDDPRRWIEEPLMFDE 383
           LNF+LAGRDTSSVAL+WFFWL+  HP +E  IL EI TVL ETRGDD   W +EPL  +E
Sbjct: 309 LNFILAGRDTSSVALSWFFWLITQHPAIEDKILREICTVLVETRGDDVALWTDEPLSCEE 368

Query: 384 ADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTVTYSIYSVGRMKSIW 443
            D+LV+LKAAL+ETLRLYPSVP D K  V DDVLPDG+FVPAGS++TYSIYS GRMKS W
Sbjct: 369 LDRLVFLKAALSETLRLYPSVPEDSKRAVKDDVLPDGTFVPAGSSITYSIYSAGRMKSTW 428

Query: 444 GEDCGEFKPDRWLS-PDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAYLQMKSIASAVLLR 503
           GEDC EFKP+RW+S  DG RF    D +KFVAFNAGPR CLGKDLAYLQMKSIASAVLLR
Sbjct: 429 GEDCLEFKPERWISQSDGGRFIN-HDPFKFVAFNAGPRICLGKDLAYLQMKSIASAVLLR 488

Query: 504 YQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRL 528
           ++L  V GH+VEQKMSLTLF K GL V +H R L
Sbjct: 489 HRLTVVTGHKVEQKMSLTLFMKYGLLVNVHERDL 512

[Close]

BLAST of CmaCh01G006540 vs. TAIR 10
Match: AT1G63710.1 (cytochrome P450, family 86, subfamily A, polypeptide 7 )

HSP 1 Score: 635.2 bits (1637), Expect = 4.7e-182
Identity = 315/513 (61.40%), Postives = 383/513 (74.66%), Query Frame = 0


Query: 24  LILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISNRRRFHDWMAGNLRSTGSAATYQT 83
           ++ LI  Y+IWF  L R+  GPRVWP++GSLPALI+N  R HD++A NLR  G   TYQT
Sbjct: 9   ILTLIVTYIIWFVSLRRSYKGPRVWPLVGSLPALITNAHRMHDFIADNLRMCG--GTYQT 68

Query: 84  CTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPKGPDWQAAFHDLLGQGIFNSDGEI 143
           C   +PF A+KQG  TVTC P+N+EH+L+TRF+NYPKGP WQ+ FHDLLG GIFNSDG+ 
Sbjct: 69  CIFPIPFLAKKQGHVTVTCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLGDGIFNSDGDT 128

Query: 144 WLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCILDKAAEYKTAVDLQDLLLRLTFDN 203
           W  QRKTAALEFTTRTLRQAM RWV+R I+ RL  IL+ A      +DLQD+LLRLTFDN
Sbjct: 129 WRFQRKTAALEFTTRTLRQAMARWVDRAIKNRLVPILESARSRAEPIDLQDVLLRLTFDN 188

Query: 204 ICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRLLYPSILWRLKKLLCIGMERRLKE 263
           ICGLTFGKDP+TLSPE P   FA+AFD ATEATL R + P  +W+++K L +G+E  +  
Sbjct: 189 ICGLTFGKDPRTLSPEFPENGFAVAFDGATEATLQRFIMPEFIWKIRKWLRLGLEDDMSR 248

Query: 264 SLKVIEEYINDAVAARK---------ESPSDDLLSRFMKKKHDGDGDGNRFSAAVLHRIA 323
           S+  ++ Y+++ +  RK         ES  DDLLSRFMKKK         +S   L  +A
Sbjct: 249 SISHVDNYLSEIINTRKLELLGQQQDESRHDDLLSRFMKKKES-------YSDKYLKYVA 308

Query: 324 LNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRGDDPRRWIEEPLMFDE 383
           LNF+LAGRDTSSVA++WFFWLV  +P VE  I+ EI T+L +TR  +  +W +EPL FDE
Sbjct: 309 LNFILAGRDTSSVAMSWFFWLVSLNPRVEEKIINEICTILIKTRDTNVSKWTDEPLTFDE 368

Query: 384 ADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTVTYSIYSVGRMKSIW 443
            D+LVYLKAAL+ETLRLYPSVP D K+VVA+DVLPDG+FVP+GS VTYSIYSVGRMK IW
Sbjct: 369 IDQLVYLKAALSETLRLYPSVPEDSKFVVANDVLPDGTFVPSGSNVTYSIYSVGRMKFIW 428

Query: 444 GEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAYLQMKSIASAVLLRY 503
           GEDC EFKP+RWL    D      + YKFVAFNAGPR CLGKDLAYLQMKSI +++LLR+
Sbjct: 429 GEDCLEFKPERWLEESRDE---KCNQYKFVAFNAGPRICLGKDLAYLQMKSITASILLRH 488

Query: 504 QLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRL 528
           +L   PGHRVEQKMSLTLF K GL++ +H R L
Sbjct: 489 RLTVAPGHRVEQKMSLTLFMKFGLKMDVHKRDL 509

[Close]

BLAST of CmaCh01G006540 vs. TAIR 10
Match: AT1G01600.1 (cytochrome P450, family 86, subfamily A, polypeptide 4 )

HSP 1 Score: 631.3 bits (1627), Expect = 6.8e-181
Identity = 320/516 (62.02%), Postives = 380/516 (73.64%), Query Frame = 0


Query: 24  LILLIFAYLIWFHFLARTLTGPRVWPVIGSLPALISNRRRFHDWMAGNLRSTGSAATYQT 83
           L+ ++  Y +WF  ++R L GPRVWP++GSLP LI  R R H+W+  NLR+ G   TYQT
Sbjct: 9   LVAIVTGYWLWFKRISRWLKGPRVWPLLGSLPGLIEQRDRMHEWITENLRACG--GTYQT 68

Query: 84  CTVALPFFARKQGFYTVTCHPRNIEHVLRTRFENYPKGPDWQAAFHDLLGQGIFNSDGEI 143
           C  A+PF A+KQG  TVTC P+N+EH+L+TRF+NYPKGP WQ+ FHDLLGQGIFNSDG+ 
Sbjct: 69  CIFAVPFLAKKQGLVTVTCDPKNLEHMLKTRFDNYPKGPTWQSVFHDLLGQGIFNSDGDT 128

Query: 144 WLVQRKTAALEFTTRTLRQAMDRWVNRTIRTRLWCILDKAAEYKTAVDLQDLLLRLTFDN 203
           WL QRKTAALEFTTRTLRQAM RWVNR I+ R   IL  A +    VDLQDL+LRLTFDN
Sbjct: 129 WLFQRKTAALEFTTRTLRQAMGRWVNRGIKLRFCPILATAQDNAEPVDLQDLILRLTFDN 188

Query: 204 ICGLTFGKDPQTLSPELPATPFALAFDSATEATLHRLLYPSILWRLKKLLCIGMERRLKE 263
           ICGL FGKD +T +P LP   FA AFD ATEA+L R + P  +W+LKK L +G+E  L  
Sbjct: 189 ICGLAFGKDTRTCAPGLPENGFASAFDRATEASLQRFIIPKFMWKLKKWLGLGLEVSLSR 248

Query: 264 SLKVIEEYINDAVAARKE-----------SPSDDLLSRFMKKKHDGDGDGNRFSAAVLHR 323
           SL  I+EY+   +  RK+              DDLLSRFM KK +       +S   L  
Sbjct: 249 SLGEIDEYLAAVINTRKQELMSQQESGTHQRHDDLLSRFMMKKTES------YSDTFLQH 308

Query: 324 IALNFVLAGRDTSSVALTWFFWLVMNHPHVEATILAEISTVLRETRG-DDPRRWIEEPLM 383
           +ALNF+LAGRDTSSVAL+WFFWL+  HP VE  I+ EI +VL ETRG DD   W EEPL 
Sbjct: 309 VALNFILAGRDTSSVALSWFFWLITMHPTVEDKIVREICSVLIETRGTDDVASWTEEPLG 368

Query: 384 FDEADKLVYLKAALAETLRLYPSVPLDFKYVVADDVLPDGSFVPAGSTVTYSIYSVGRMK 443
           FDE D+LVYLKAA++ETLRLYPSVP D K+V  DDVLPDG+FVPAGS+VTYSIY+ GRMK
Sbjct: 369 FDEIDRLVYLKAAISETLRLYPSVPEDSKHVENDDVLPDGTFVPAGSSVTYSIYAAGRMK 428

Query: 444 SIWGEDCGEFKPDRWLSPDGDRFEGPKDGYKFVAFNAGPRTCLGKDLAYLQMKSIASAVL 503
           S WGEDC EF P+RW+SP   +F    D Y+FVAFNAGPR CLGKDLAYLQMK+IA+AVL
Sbjct: 429 STWGEDCLEFNPERWISPIDGKFIN-HDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVL 488

Query: 504 LRYQLAPVPGHRVEQKMSLTLFTKNGLRVYLHPRRL 528
           LR++L  VPGH+VEQKMSLTLF KNGL V L+ R L
Sbjct: 489 LRHRLTVVPGHKVEQKMSLTLFMKNGLLVNLYKRDL 515

The following BLAST results are available for this feature:

BLAST of CmaCh01G006540 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P48422	6.6e-221	73.45	Cytochrome P450 86A1 OS=Arabidopsis thaliana OX=3702 GN=CYP86A1 PE=1 SV=2	[more]
B3RFJ6	9.6e-188	62.60	Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1	[more]
O23066	5.8e-185	63.42	Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1	[more]
O80823	9.9e-185	63.23	Cytochrome P450 86A8 OS=Arabidopsis thaliana OX=3702 GN=CYP86A8 PE=2 SV=1	[more]
Q9CAD6	6.7e-181	61.40	Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1	[more]

BLAST of CmaCh01G006540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K9B5	0.0e+00	100.00	cytochrome P450 86A1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492855...	[more]
A0A6J1K6Z0	1.3e-304	98.11	cytochrome P450 86A1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492855...	[more]
A0A6J1GB79	6.5e-296	95.27	cytochrome P450 86A1-like OS=Cucurbita moschata OX=3662 GN=LOC111452582 PE=3 SV=...	[more]
A0A0A0LV45	4.4e-268	86.77	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G528600 PE=3 SV=1	[more]
A0A6J1HZR9	1.3e-267	86.74	cytochrome P450 86A1-like OS=Cucurbita maxima OX=3661 GN=LOC111469581 PE=3 SV=1	[more]

BLAST of CmaCh01G006540 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022998106.1	0.0e+00	100.00	cytochrome P450 86A1-like isoform X1 [Cucurbita maxima]	[more]
XP_022998107.1	2.7e-304	98.11	cytochrome P450 86A1-like isoform X2 [Cucurbita maxima]	[more]
KAG7036979.1	1.5e-299	96.40	Cytochrome P450 86A1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6607300.1	1.2e-296	95.64	Cytochrome P450 86A1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022949142.1	1.3e-295	95.27	cytochrome P450 86A1-like [Cucurbita moschata]	[more]

BLAST of CmaCh01G006540 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G58860.1	4.7e-222	73.45	cytochrome P450, family 86, subfamily A, polypeptide 1	[more]
AT4G00360.1	4.1e-186	63.42	cytochrome P450, family 86, subfamily A, polypeptide 2	[more]
AT2G45970.1	7.1e-186	63.23	cytochrome P450, family 86, subfamily A, polypeptide 8	[more]
AT1G63710.1	4.7e-182	61.40	cytochrome P450, family 86, subfamily A, polypeptide 7	[more]
AT1G01600.1	6.8e-181	62.02	cytochrome P450, family 86, subfamily A, polypeptide 4	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 382..400 score: 30.14 coord: 463..473 score: 45.27 coord: 329..355 score: 27.99 coord: 473..496 score: 24.05 coord: 309..326 score: 29.36
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 473..484 score: 38.77 coord: 320..337 score: 33.23 coord: 383..394 score: 43.94 coord: 464..473 score: 48.19
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 99..513 e-value: 3.8E-65 score: 220.4
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 38..527 e-value: 1.2E-97 score: 329.2
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 34..525
None	No IPR available	PANTHER	PTHR24296	CYTOCHROME P450	coord: 16..526
None	No IPR available	PANTHER	PTHR24296:SF145	CYTOCHROME P450 FAMILY-DEPENDENT FATTY ACID HYDROXYLASE	coord: 16..526
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 466..475

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh01G006540.1	CmaCh01G006540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen