CmaCh01G005860 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh01G005860
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionGTP cyclohydrolase II
LocationCma_Chr01: 3057987 .. 3060865 (+)
RNA-Seq ExpressionCmaCh01G005860
SyntenyCmaCh01G005860
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTGCTCTTCTTGCTCATCCTCTCCTTCCCCGTTTCACTAACTTACCTCTTTCAAGGTACGTTTCTTTCCCTACTCACCCCTAATTTTGTGCCCCTTTATGTGTCATAATAAAGTTGTTCTCTTTGTAGCTTTCATCGCCCCGCTTTGCCTCATCCTAAGATTGGCAAATGTAAGCAAAGAGGGCTGCGTTTTGTTTGCTCCGCTGCTGCTGACTTGTTTGATGACAACCTCGCCGCTAAAAATGGTAACGGTGCTGCTTCCGCGGCTGCTTTAGGACTTGAGGCTGAAACCACGCCCGATGTTGTTGATTTCTTTGATGATGAGGACGATCCTGACTGCCCATCTGAGGGCTACTCTTCCATTCCCACCGCCGTTGATGCTATTAGCCAAGGAAAGGTTGCCAAATATCCACTTCCTTATGGATAATGCTTGCTTATACTCTAATAAATTATACTTTCTTTTTTCTCCCTTGTCCATTTCTCTCCCACAAAAGTCAAAGTCTACCCTAACAACAAATATGATTCTCTTTTCAAGTTTTTTCTTTACCGTTTCTCCTCATAGTTTTTTTAAAAGTATTGGTAGTGAGAGGTTTCCACGTACTTATGAAGAATGTTACGTTCTCTTCTTCGATCGATGTCGGATCTTACAATCCACCCCCTTCGAGGCCCAACGTCCTTCCTAGCACATTGCCTTGTGTCCCACCCTTTCGAACTTCAACCTCTTCCCTGACACATCGCTCAGTGTCTGTCTCTGATACCATTTGTAACACTGCAGACCAGCTAACACATATTGTCCTTTTTGGGCTTTCCCTTTCGAGTTTCTCCTTAATGTTTTTAAAACCTACTTGCTAGGGAGGAGAAGTTTTCTCACCCTCATAAAGGATGTTTCGTTCTCCTCTCATACCGATGTGTGATCTCACATGGTTACAAAGAACAAAAATTTTATTCTCTCTATACTTATTATTTGAAGGATGTGGCATGTTTTTATCTTACCCCTTTTTGCATGCATTTTTATTCTCTCTCCTCATGCATTTCTCTTCTCCTAATGGCCGAAAAACTTAATTGTTTTCTAGTTTGTGATCGTTGTGGATGATGAAAATGAGGACACGGAAGGGAATTTGGTAATGGCTGCCTCTTTTACAACTCCCAGTAATATAACAACCATGGTTCAACATGGCTCAGGCATACTCTATGTGGGTATGAAAGAGGACGATCTTGAAAGGCTTAAGCTTCCCATAATATCGATCGATAATGGGAACGAGACATCAACTTTTACCATCACTGTGGTAGGTAGTACGCATGAGAAATTTGAACTTCTGACCTCTTGATTAAATTATTATACACGATCACACCCACCTAGGGATGTTACTTTGAGTGTTAGTTATTAATTTATTGTTTGAATGAGACAGGATTTGAAAAATGGGACATCAGCAGGAGTATCAACAGCAGATAGAGCAAAGACAATTGTTGCTTTATCATCACCTCAGTCTAAGGCTGAGGACTTCAGACGGCCAGGTCACGTGTTTCCCCTCCGGTACCGAGACGGCGGCGTTCTGAGGAGAGCTGGCCACACGGAAGCTTCTGTAGATTTAGTTTCCCTTGCTGGGTTAAGGTCAGTTTCTGTTCTTTCAACCATTGTTGACCCTGAAGATGGCTCCATGGCTTCACTACCCAAGTTGAGAAAGTTGGCATCAGAAAAGAATATCCCAATAGTCTCTATTACTGATTTGATTAGGTAAGTTAGGTTGATTCATCGTAATTGATATCTTATGCGTTGAACTATAAAAAATTTGAGTCGGGTTTGGATTCACTTAATTTTTTGGCTGTTTATTTTGGTAGATACCGAAGAAAGAGAGAAAATTTGATTGAAAGGATTGCTATTTCTCGGCTGCCAACTAAATGGGGCCAATTCTATGCTCATTGCTATCGCTCAAATCTAGATGGAATTGAGCACGTGGCTCTTGTAAAGGTAATGGAATTTGTTTTCTTTGAGGAATAATGCATTCATTAACTCATGACATCCTTAAATATCAAAAACATATGATAGTTGGTGGTCGGTGAGCTTCAAAACCCAATATTTAACCTAAAAAATATTTGCTCTGAGATGAGTTAAATCTTTATTGAAATCCAAAATCTTAAGGATATTTAGGGTTAGAATTATGATTTACAATATATATATATCATAAAGACATCTATTTATTATAATTTTTTTTTTTATAATTTATTTGATTATAAATAAAAAACTTTCACCATGATTTATTTCAACAGGGAAACATTGGAGACGGTCAAGATGTGCTTGTAAGGGTCCATTCCGAGTGCTTAACTGGCGACATATTCGCGTCAGCAAGATGTGACTGTGGCAACCAACTAGACTTAGCAATGCAAATGATTGAGAAGGCTGGAAGAGGCGCTGTTATTTACCTAAGAGGTCATGAAGGAAGAGGCATTGGCCTTGGCCCCAAGCTGCTTGCCTACAACCTACAAGATCAAGGCCACGACACAGTCGAAGCCAACATCGAGCTCGGATTGCCTGCCGACGCCCGTGATTATGGAGTCGGAGCTCAGGTCAGTCAACTCAACTCGATTTGACTAGTTAATAGATAAGTTAAATTTGATTTTATTTTTTTATTTGTGGATGATGTCAGATGCTGAGAGATATTGGGATTAGAACAATGCGGTTGATGACAAACAACCCCGCGAAGTTCAAGGGGCTGAAGGGATATGGTTTGGCAGTGACAGCGAGGGTCCCTGTGGTGACACCCATTACGGATGAAAACAGAAAGTACTTGGAAACCAAGAGAACCAAGATGGGCCATATCTATGCGAAGGGCCAATTTTTAAGGTCGCGACAGGCTGAAGCTTCAAGAAATTGA

mRNA sequence

ATGGCTTCTGCTCTTCTTGCTCATCCTCTCCTTCCCCGTTTCACTAACTTACCTCTTTCAAGCTTTCATCGCCCCGCTTTGCCTCATCCTAAGATTGGCAAATGTAAGCAAAGAGGGCTGCGTTTTGTTTGCTCCGCTGCTGCTGACTTGTTTGATGACAACCTCGCCGCTAAAAATGGTAACGGTGCTGCTTCCGCGGCTGCTTTAGGACTTGAGGCTGAAACCACGCCCGATGTTGTTGATTTCTTTGATGATGAGGACGATCCTGACTGCCCATCTGAGGGCTACTCTTCCATTCCCACCGCCGTTGATGCTATTAGCCAAGGAAAGTTTGTGATCGTTGTGGATGATGAAAATGAGGACACGGAAGGGAATTTGGTAATGGCTGCCTCTTTTACAACTCCCAGTAATATAACAACCATGGTTCAACATGGCTCAGGCATACTCTATGTGGGTATGAAAGAGGACGATCTTGAAAGGCTTAAGCTTCCCATAATATCGATCGATAATGGGAACGAGACATCAACTTTTACCATCACTGTGGATTTGAAAAATGGGACATCAGCAGGAGTATCAACAGCAGATAGAGCAAAGACAATTGTTGCTTTATCATCACCTCAGTCTAAGGCTGAGGACTTCAGACGGCCAGGTCACGTGTTTCCCCTCCGGTACCGAGACGGCGGCGTTCTGAGGAGAGCTGGCCACACGGAAGCTTCTGTAGATTTAGTTTCCCTTGCTGGGTTAAGGTCAGTTTCTGTTCTTTCAACCATTGTTGACCCTGAAGATGGCTCCATGGCTTCACTACCCAAGTTGAGAAAGTTGGCATCAGAAAAGAATATCCCAATAGTCTCTATTACTGATTTGATTAGATACCGAAGAAAGAGAGAAAATTTGATTGAAAGGATTGCTATTTCTCGGCTGCCAACTAAATGGGGCCAATTCTATGCTCATTGCTATCGCTCAAATCTAGATGGAATTGAGCACGTGGCTCTTGTAAAGGGAAACATTGGAGACGGTCAAGATGTGCTTGTAAGGGTCCATTCCGAGTGCTTAACTGGCGACATATTCGCGTCAGCAAGATGTGACTGTGGCAACCAACTAGACTTAGCAATGCAAATGATTGAGAAGGCTGGAAGAGGCGCTGTTATTTACCTAAGAGGTCATGAAGGAAGAGGCATTGGCCTTGGCCCCAAGCTGCTTGCCTACAACCTACAAGATCAAGGCCACGACACAGTCGAAGCCAACATCGAGCTCGGATTGCCTGCCGACGCCCGTGATTATGGAGTCGGAGCTCAGATGCTGAGAGATATTGGGATTAGAACAATGCGGTTGATGACAAACAACCCCGCGAAGTTCAAGGGGCTGAAGGGATATGGTTTGGCAGTGACAGCGAGGGTCCCTGTGGTGACACCCATTACGGATGAAAACAGAAAGTACTTGGAAACCAAGAGAACCAAGATGGGCCATATCTATGCGAAGGGCCAATTTTTAAGGTCGCGACAGGCTGAAGCTTCAAGAAATTGA

Coding sequence (CDS)

ATGGCTTCTGCTCTTCTTGCTCATCCTCTCCTTCCCCGTTTCACTAACTTACCTCTTTCAAGCTTTCATCGCCCCGCTTTGCCTCATCCTAAGATTGGCAAATGTAAGCAAAGAGGGCTGCGTTTTGTTTGCTCCGCTGCTGCTGACTTGTTTGATGACAACCTCGCCGCTAAAAATGGTAACGGTGCTGCTTCCGCGGCTGCTTTAGGACTTGAGGCTGAAACCACGCCCGATGTTGTTGATTTCTTTGATGATGAGGACGATCCTGACTGCCCATCTGAGGGCTACTCTTCCATTCCCACCGCCGTTGATGCTATTAGCCAAGGAAAGTTTGTGATCGTTGTGGATGATGAAAATGAGGACACGGAAGGGAATTTGGTAATGGCTGCCTCTTTTACAACTCCCAGTAATATAACAACCATGGTTCAACATGGCTCAGGCATACTCTATGTGGGTATGAAAGAGGACGATCTTGAAAGGCTTAAGCTTCCCATAATATCGATCGATAATGGGAACGAGACATCAACTTTTACCATCACTGTGGATTTGAAAAATGGGACATCAGCAGGAGTATCAACAGCAGATAGAGCAAAGACAATTGTTGCTTTATCATCACCTCAGTCTAAGGCTGAGGACTTCAGACGGCCAGGTCACGTGTTTCCCCTCCGGTACCGAGACGGCGGCGTTCTGAGGAGAGCTGGCCACACGGAAGCTTCTGTAGATTTAGTTTCCCTTGCTGGGTTAAGGTCAGTTTCTGTTCTTTCAACCATTGTTGACCCTGAAGATGGCTCCATGGCTTCACTACCCAAGTTGAGAAAGTTGGCATCAGAAAAGAATATCCCAATAGTCTCTATTACTGATTTGATTAGATACCGAAGAAAGAGAGAAAATTTGATTGAAAGGATTGCTATTTCTCGGCTGCCAACTAAATGGGGCCAATTCTATGCTCATTGCTATCGCTCAAATCTAGATGGAATTGAGCACGTGGCTCTTGTAAAGGGAAACATTGGAGACGGTCAAGATGTGCTTGTAAGGGTCCATTCCGAGTGCTTAACTGGCGACATATTCGCGTCAGCAAGATGTGACTGTGGCAACCAACTAGACTTAGCAATGCAAATGATTGAGAAGGCTGGAAGAGGCGCTGTTATTTACCTAAGAGGTCATGAAGGAAGAGGCATTGGCCTTGGCCCCAAGCTGCTTGCCTACAACCTACAAGATCAAGGCCACGACACAGTCGAAGCCAACATCGAGCTCGGATTGCCTGCCGACGCCCGTGATTATGGAGTCGGAGCTCAGATGCTGAGAGATATTGGGATTAGAACAATGCGGTTGATGACAAACAACCCCGCGAAGTTCAAGGGGCTGAAGGGATATGGTTTGGCAGTGACAGCGAGGGTCCCTGTGGTGACACCCATTACGGATGAAAACAGAAAGTACTTGGAAACCAAGAGAACCAAGATGGGCCATATCTATGCGAAGGGCCAATTTTTAAGGTCGCGACAGGCTGAAGCTTCAAGAAATTGA

Protein sequence

MASALLAHPLLPRFTNLPLSSFHRPALPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNGNGAASAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIPTAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTITVDLKNGTSAGVSTADRAKTIVALSSPQSKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRSVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIYAKGQFLRSRQAEASRN
Homology
BLAST of CmaCh01G005860 vs. ExPASy Swiss-Prot
Match: Q9FN89 (Monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIBA3 PE=1 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 1.6e-179
Identity = 313/449 (69.71%), Postives = 380/449 (84.63%), Query Frame = 0

Query: 49  DLFDDNLAAK-NGNGAASAAALGLEAETTPDVVDFF--DDEDDPDCPSEGYSSIPTAVDA 108
           ++FD+N   K +G+   SA+   ++AE TP+  DFF  D E DPDCP++GYSSI  A+ A
Sbjct: 51  NVFDENPVRKTDGSLFDSASFETVDAEITPETDDFFVSDAEGDPDCPTQGYSSIELALQA 110

Query: 109 ISQGKFVIVVDDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPI 168
           + +GKFVIVVDDE  D EGNL+MAA+ T+P +I  ++++GSGI+ VGMK+++LERL L +
Sbjct: 111 LRKGKFVIVVDDETGDVEGNLIMAATLTSPKDIAFLIKNGSGIVSVGMKKENLERLSLTL 170

Query: 169 ISIDNGNETS---TFTITVDLKNGTSAGVSTADRAKTIVALSSPQSKAEDFRRPGHVFPL 228
           +S +  +E S   TFTITVD K+GTS GVS +DRA T++ALSS  +K +DFRRPGHVFPL
Sbjct: 171 MSPEMEDEDSSAPTFTITVDAKSGTSTGVSASDRAMTVLALSSLDAKPDDFRRPGHVFPL 230

Query: 229 RYRDGGVLRRAGHTEASVDLVSLAGLRSVSVLSTIVDPEDGSMASLPKLRKLASEKNIPI 288
           +YRDGGVLRRAGHTEASVDL+ LAGLR +SVLS I+D EDGSMASLP ++KLA+E +IPI
Sbjct: 231 KYRDGGVLRRAGHTEASVDLMILAGLRPLSVLSAILDQEDGSMASLPYMKKLATEHDIPI 290

Query: 289 VSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDV 348
           VS+TDLIRYRRKR+ L+ERI +SRLPTKWG F A+CYRS LDG E++ALVKGN+G+G+D+
Sbjct: 291 VSLTDLIRYRRKRDKLVERITVSRLPTKWGLFQAYCYRSKLDGTENIALVKGNVGNGEDI 350

Query: 349 LVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAY 408
           LVRVHSECLTGDIF SARCDCGNQLDLAM++IEK GRG V+YLRGHEGRGIGLG KL AY
Sbjct: 351 LVRVHSECLTGDIFGSARCDCGNQLDLAMELIEKEGRGVVVYLRGHEGRGIGLGHKLRAY 410

Query: 409 NLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAV 468
           NLQD+GHDTV+AN+ELGL  D+R+YG+GAQMLRDIG+RTMRLMTNNPAKF GLKGYGLAV
Sbjct: 411 NLQDEGHDTVQANVELGLSIDSREYGIGAQMLRDIGVRTMRLMTNNPAKFTGLKGYGLAV 470

Query: 469 TARVPVVTPITDENRKYLETKRTKMGHIY 492
             RVPVVTPIT ENR+Y+ETKR KMGHIY
Sbjct: 471 VGRVPVVTPITKENRRYMETKRKKMGHIY 499

BLAST of CmaCh01G005860 vs. ExPASy Swiss-Prot
Match: Q6L506 (Probable monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RIBA3 PE=2 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 1.4e-167
Identity = 292/438 (66.67%), Postives = 361/438 (82.42%), Query Frame = 0

Query: 60  GNGAASAAALGLEAETTPDVVDFF--DDEDDPDCPSEGYSSIPTAVDAISQGKFVIVVDD 119
           G   +SA+   + A+ T +  DFF  D E DPD P++G+SSI  A+ A+ +GKFVI VDD
Sbjct: 71  GFNGSSASFRTVGAKITQETGDFFVSDAEGDPDKPTDGFSSIDEAIGALHEGKFVIAVDD 130

Query: 120 ENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIIS----IDNGNE 179
           E+ D EG+LVMAA+   P +I  M+++GSGI+ VGMKE+DL RL +P++S    I++ + 
Sbjct: 131 ESGDNEGDLVMAATLADPESIAFMIRNGSGIISVGMKEEDLTRLMIPMMSPIAEIEDISA 190

Query: 180 TSTFTITVDLKNGTSAGVSTADRAKTIVALSSPQSKAEDFRRPGHVFPLRYRDGGVLRRA 239
            ++ T+TVD + G S GVS ADRAKTI  L+SP SK  D RRPGH+FPL+YR+GGVL+RA
Sbjct: 191 AAS-TVTVDARVGISTGVSAADRAKTIFTLASPDSKPTDLRRPGHIFPLKYRNGGVLKRA 250

Query: 240 GHTEASVDLVSLAGLRSVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRR 299
           GHTEASVDLV+LAGLR VSVLST+++P DGSMA +P L+++A E +IPIVSI DLIRYRR
Sbjct: 251 GHTEASVDLVALAGLRPVSVLSTVINPVDGSMAGMPVLKQMALEHDIPIVSIADLIRYRR 310

Query: 300 KRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTG 359
           KRE L+E IA+SRLPTKWG F A+CY+S LDG EH+A+ KG+IGDG+DVLVRVHSECLTG
Sbjct: 311 KREKLVELIAVSRLPTKWGLFRAYCYQSKLDGTEHIAVAKGDIGDGEDVLVRVHSECLTG 370

Query: 360 DIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVE 419
           DI  SARCDCGNQLDLAMQ+I+KAGRG ++YLRGHEGRGIGLG KL AYNLQD GHDTV+
Sbjct: 371 DILGSARCDCGNQLDLAMQLIDKAGRGVLVYLRGHEGRGIGLGQKLRAYNLQDDGHDTVQ 430

Query: 420 ANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPIT 479
           AN+ELGL  D+R+YG+GAQ+LRD+G+RTMRLMTNNPAKF GLKGYGLAV  RVPV++PIT
Sbjct: 431 ANVELGLAVDSREYGIGAQILRDMGVRTMRLMTNNPAKFVGLKGYGLAVVGRVPVISPIT 490

Query: 480 DENRKYLETKRTKMGHIY 492
            EN++YLETKRTKMGH+Y
Sbjct: 491 KENQRYLETKRTKMGHVY 507

BLAST of CmaCh01G005860 vs. ExPASy Swiss-Prot
Match: Q6Z234 (Probable bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RIBA1 PE=2 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 1.4e-164
Identity = 284/434 (65.44%), Postives = 349/434 (80.41%), Query Frame = 0

Query: 64  ASAAALGLEAETTPDVVDFF---DDEDDPDCPSEGYSSIPTAVDAISQGKFVIVVDDENE 123
           A AAALG  A     VVD F   DDE D D P+EG+SSIP A++ I QGK+VIVVDDE+ 
Sbjct: 75  ADAAALGTIAADMAPVVDGFSADDDELDLDSPTEGFSSIPEAIEDIRQGKYVIVVDDEDR 134

Query: 124 DTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNE---TSTF 183
           + EG+L+MAAS  TP  +  +V+HG+GI+ V MKEDDLERL+LP++     NE    + F
Sbjct: 135 ENEGDLIMAASKVTPEAMAFIVRHGTGIVCVSMKEDDLERLELPLMVTTKENEEKLRTAF 194

Query: 184 TITVDLKNGTSAGVSTADRAKTIVALSSPQSKAEDFRRPGHVFPLRYRDGGVLRRAGHTE 243
           T++VD K GT+ GVS  DRA T++AL+SP SK EDF RPGH+FPL+YR+GGVL+RAGHTE
Sbjct: 195 TVSVDAKEGTTTGVSAKDRANTVLALASPNSKPEDFNRPGHIFPLKYREGGVLKRAGHTE 254

Query: 244 ASVDLVSLAGLRSVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKREN 303
           ASVDL  LAGL   +VL  IVD +DGSMA LPKL+  A  +N+ I+SI DLIRYRRKR+ 
Sbjct: 255 ASVDLAMLAGLPPAAVLCEIVDDDDGSMALLPKLQDFARRENLKIISIADLIRYRRKRDR 314

Query: 304 LIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFA 363
           L+ER+ ++ L  +WG F ++CYRS +DG+EH+A+VKG++GDGQD+LVRVHSECLTGDIF 
Sbjct: 315 LVERVCVTPLQLQWGSFQSYCYRSLIDGMEHIAMVKGDVGDGQDILVRVHSECLTGDIFG 374

Query: 364 SARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIE 423
           SARCDCGNQL LAM MIEK GRG V+YLRGHEGRGIGLG KL AYNLQD G DTVEAN +
Sbjct: 375 SARCDCGNQLALAMTMIEKTGRGVVVYLRGHEGRGIGLGHKLRAYNLQDDGRDTVEANED 434

Query: 424 LGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENR 483
           LGLP D+R+YG+GAQ+LRD+G+RTMRLMTNNPAK+ GLKGYGL+V  RVP++TPIT+ENR
Sbjct: 435 LGLPVDSREYGIGAQILRDLGVRTMRLMTNNPAKYTGLKGYGLSVLGRVPLLTPITNENR 494

Query: 484 KYLETKRTKMGHIY 492
           +Y+ETKR KMGH+Y
Sbjct: 495 RYMETKRLKMGHVY 508

BLAST of CmaCh01G005860 vs. ExPASy Swiss-Prot
Match: Q0E079 (Probable bifunctional riboflavin biosynthesis protein RIBA 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RIBA2 PE=2 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 5.6e-161
Identity = 282/427 (66.04%), Postives = 341/427 (79.86%), Query Frame = 0

Query: 79  VVDFFDDED-----DPDCPSEGYSSIPTAVDAISQGKFVIVVDDENEDTEGNLVMAASFT 138
           V D F DED     D D P+EG+SSI  A+  I QGK VIVVDDE+ + EG+L+MAAS  
Sbjct: 114 VADMFSDEDKDTEQDLDSPTEGFSSISEAIKDIQQGKLVIVVDDESRENEGDLIMAASLV 173

Query: 139 TPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNE---TSTFTITVDLKNGTSAG 198
           TP  +  +V++G+GI+ V MKE+DLERL LP++     NE    + FT+TVD K GT+ G
Sbjct: 174 TPEAMAFVVRYGTGIVCVSMKEEDLERLNLPLMVATKENEEKLCTAFTVTVDAKEGTTTG 233

Query: 199 VSTADRAKTIVALSSPQSKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRS 258
           VS  DRAKT++ L+SP SK EDF RPGH+FPL+YR+GGVL+RAGHTEASVDL  LAGL  
Sbjct: 234 VSAKDRAKTVMTLASPDSKPEDFNRPGHIFPLKYREGGVLKRAGHTEASVDLAMLAGLPP 293

Query: 259 VSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTK 318
            +VL  IVD EDGSMA LPKLR  A  +N+ IVSI DLIRYRRKR+ L+ER +++RLP +
Sbjct: 294 AAVLCEIVD-EDGSMARLPKLRVFAERENLKIVSIADLIRYRRKRDRLVERSSVARLPLR 353

Query: 319 WGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFASARCDCGNQLDLA 378
           WG   A+CYRS +DGIEH+A+VKG IGDGQ VLVRVHSECLTGDIF SARCDCG+QL +A
Sbjct: 354 WGNVRAYCYRSVIDGIEHIAMVKGEIGDGQGVLVRVHSECLTGDIFGSARCDCGDQLAMA 413

Query: 379 MQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVG 438
           M+MIEKAGRG ++YLRGHEGRGIGLG KL AYNLQD G DTVEAN +LGLP D+R+YG+G
Sbjct: 414 MEMIEKAGRGVLVYLRGHEGRGIGLGHKLRAYNLQDDGRDTVEANEDLGLPVDSREYGIG 473

Query: 439 AQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHI 496
           AQ+LRD+G+R+M+LMTNNPAK+ GLKGYGL++  RVP+VTPIT ENR+YLETKRTKMGH+
Sbjct: 474 AQILRDLGVRSMKLMTNNPAKYGGLKGYGLSIVGRVPLVTPITSENRRYLETKRTKMGHV 533

BLAST of CmaCh01G005860 vs. ExPASy Swiss-Prot
Match: P47924 (Bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIBA1 PE=1 SV=2)

HSP 1 Score: 565.8 bits (1457), Expect = 4.8e-160
Identity = 301/527 (57.12%), Postives = 376/527 (71.35%), Query Frame = 0

Query: 6   LAHPLLPRFTNLPLSSFHRPALP--HPKIGKCKQRGLRFVCSAAADLFDDNLAAKNGNG- 65
           L+   L + +   L   HR  LP  HP      +     V +A     DD L+  NGN  
Sbjct: 16  LSRSRLSQSSTTLLHGLHRVTLPSNHPLSTFSIKTNTGKVKAAVISREDDLLSFTNGNTP 75

Query: 66  ---------------AASAAALG-LEAETTPDVVDFFDDEDDP---DCPSEGYSSIPTAV 125
                           A + +LG L A++ P   + F  EDD    D P+ G+SSIP A+
Sbjct: 76  LSNGSLIDDRTEEPLEADSVSLGTLAADSAPAPANGFVAEDDDFELDLPTPGFSSIPEAI 135

Query: 126 DAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKL 185
           + I QGK V+VVDDE+ + EG+LVMAA   TP  +  +V+HG+GI+ V MKEDDLERL L
Sbjct: 136 EDIRQGKLVVVVDDEDRENEGDLVMAAQLATPEAMAFIVRHGTGIVCVSMKEDDLERLHL 195

Query: 186 PIISIDNGNE---TSTFTITVDLKNGTSAGVSTADRAKTIVALSSPQSKAEDFRRPGHVF 245
           P++     NE   ++ FT+TVD K+GT+ GVS  DRA TI++L+S  SK EDF RPGH+F
Sbjct: 196 PLMVNQKENEEKLSTAFTVTVDAKHGTTTGVSARDRATTILSLASRDSKPEDFNRPGHIF 255

Query: 246 PLRYRDGGVLRRAGHTEASVDLVSLAGLRSVSVLSTIVDPEDGSMASLPKLRKLASEKNI 305
           PL+YR+GGVL+RAGHTEASVDL  LAGL  V VL  IVD +DGSMA LPKLR+ A+E N+
Sbjct: 256 PLKYREGGVLKRAGHTEASVDLTVLAGLDPVGVLCEIVD-DDGSMARLPKLREFAAENNL 315

Query: 306 PIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQ 365
            +VSI DLIRYRRKR+ L+ER + +R+PT WG F A+CYRS LDGIEH+A+VKG IGDGQ
Sbjct: 316 KVVSIADLIRYRRKRDKLVERASAARIPTMWGPFTAYCYRSILDGIEHIAMVKGEIGDGQ 375

Query: 366 DVLVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLL 425
           D+LVRVHSECLTGDIF SARCDCGNQL L+MQ IE  GRG ++YLRGHEGRGIGLG KL 
Sbjct: 376 DILVRVHSECLTGDIFGSARCDCGNQLALSMQQIEATGRGVLVYLRGHEGRGIGLGHKLR 435

Query: 426 AYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGL 485
           AYNLQD G DTVEAN ELGLP D+R+YG+GAQ++RD+G+RTM+LMTNNPAK+ GLKGYGL
Sbjct: 436 AYNLQDAGRDTVEANEELGLPVDSREYGIGAQIIRDLGVRTMKLMTNNPAKYVGLKGYGL 495

Query: 486 AVTARVPVVTPITDENRKYLETKRTKMGHIYA---KGQFLRSRQAEA 505
           A+  RVP+++ IT EN++YLETKRTKMGH+Y    KG  +   ++E+
Sbjct: 496 AIVGRVPLLSLITKENKRYLETKRTKMGHMYGLKFKGDVVEKIESES 541

BLAST of CmaCh01G005860 vs. ExPASy TrEMBL
Match: A0A6J1KB55 (GTP cyclohydrolase II OS=Cucurbita maxima OX=3661 GN=LOC111493343 PE=3 SV=1)

HSP 1 Score: 1001.5 bits (2588), Expect = 1.3e-288
Identity = 507/507 (100.00%), Postives = 507/507 (100.00%), Query Frame = 0

Query: 1   MASALLAHPLLPRFTNLPLSSFHRPALPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNG 60
           MASALLAHPLLPRFTNLPLSSFHRPALPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNG
Sbjct: 1   MASALLAHPLLPRFTNLPLSSFHRPALPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNG 60

Query: 61  NGAASAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIPTAVDAISQGKFVIVVDDENE 120
           NGAASAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIPTAVDAISQGKFVIVVDDENE
Sbjct: 61  NGAASAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIPTAVDAISQGKFVIVVDDENE 120

Query: 121 DTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTIT 180
           DTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTIT
Sbjct: 121 DTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTIT 180

Query: 181 VDLKNGTSAGVSTADRAKTIVALSSPQSKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASV 240
           VDLKNGTSAGVSTADRAKTIVALSSPQSKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASV
Sbjct: 181 VDLKNGTSAGVSTADRAKTIVALSSPQSKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASV 240

Query: 241 DLVSLAGLRSVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRENLIE 300
           DLVSLAGLRSVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRENLIE
Sbjct: 241 DLVSLAGLRSVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRENLIE 300

Query: 301 RIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFASAR 360
           RIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFASAR
Sbjct: 301 RIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFASAR 360

Query: 361 CDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGL 420
           CDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGL
Sbjct: 361 CDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGL 420

Query: 421 PADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYL 480
           PADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYL
Sbjct: 421 PADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYL 480

Query: 481 ETKRTKMGHIYAKGQFLRSRQAEASRN 508
           ETKRTKMGHIYAKGQFLRSRQAEASRN
Sbjct: 481 ETKRTKMGHIYAKGQFLRSRQAEASRN 507

BLAST of CmaCh01G005860 vs. ExPASy TrEMBL
Match: A0A6J1G8U4 (GTP cyclohydrolase II OS=Cucurbita moschata OX=3662 GN=LOC111452026 PE=3 SV=1)

HSP 1 Score: 976.1 bits (2522), Expect = 5.6e-281
Identity = 497/511 (97.26%), Postives = 501/511 (98.04%), Query Frame = 0

Query: 1   MASALLAHPLLPRFTNLPLSSFHRPALPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNG 60
           MAS LL+HPLLPRFTNLPLSSF+RPALPHPKIGKCK RGLRFVCSAAADLFDDNLAAKNG
Sbjct: 1   MASTLLSHPLLPRFTNLPLSSFNRPALPHPKIGKCKLRGLRFVCSAAADLFDDNLAAKNG 60

Query: 61  NG----AASAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIPTAVDAISQGKFVIVVD 120
           NG    AA+AAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSI  AVDAISQGKFVIVVD
Sbjct: 61  NGAAAAAAAAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIQNAVDAISQGKFVIVVD 120

Query: 121 DENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETST 180
           DENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETST
Sbjct: 121 DENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETST 180

Query: 181 FTITVDLKNGTSAGVSTADRAKTIVALSSPQSKAEDFRRPGHVFPLRYRDGGVLRRAGHT 240
           FTITVDLKNGTSAGVSTADRAKTIVALSSP+SKAEDFRRPGHVFPLRYRDGGVLRRAGHT
Sbjct: 181 FTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHT 240

Query: 241 EASVDLVSLAGLRSVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRE 300
           EASVDLVSLAGLR VSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRE
Sbjct: 241 EASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRE 300

Query: 301 NLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIF 360
           NLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIF
Sbjct: 301 NLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIF 360

Query: 361 ASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANI 420
           ASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANI
Sbjct: 361 ASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANI 420

Query: 421 ELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDEN 480
           ELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDEN
Sbjct: 421 ELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDEN 480

Query: 481 RKYLETKRTKMGHIYAKGQFLRSRQAEASRN 508
           RKYLETKRTKMGHIYAKGQFLRSRQAEAS N
Sbjct: 481 RKYLETKRTKMGHIYAKGQFLRSRQAEASTN 511

BLAST of CmaCh01G005860 vs. ExPASy TrEMBL
Match: A0A6J1DY85 (GTP cyclohydrolase II OS=Momordica charantia OX=3673 GN=LOC111025642 PE=3 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 1.0e-226
Identity = 410/503 (81.51%), Postives = 443/503 (88.07%), Query Frame = 0

Query: 1   MASALLAHPLLPRFTNLPLSSF-HRPAL---PHPKIGKCKQRGLRFVCSAAA----DLFD 60
           M SALL HPL  RF NL LS+F +RP+    P   + K KQR      SAAA    ++FD
Sbjct: 1   MDSALLCHPLFTRFNNLTLSTFNNRPSFAPPPPQTLRKSKQRTSNSASSAAAFSAGNMFD 60

Query: 61  DNLAAKNGNGAASAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIPTAVDAISQGKFV 120
           +    KNG  + +A+ LGL AET+ + +DFFD+ +DPD PS+GYSSI +AVDAI QGKFV
Sbjct: 61  ETPTTKNGKPSVAASTLGLGAETSSEAIDFFDNLEDPDHPSDGYSSIQSAVDAIHQGKFV 120

Query: 121 IVVDDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNG- 180
           IVVDDEN D+EGNLVMAASF TP N+T+MVQHGSGI+YVGMKEDDL+RLKLP+ S+DNG 
Sbjct: 121 IVVDDENGDSEGNLVMAASFATPGNVTSMVQHGSGIVYVGMKEDDLQRLKLPLFSVDNGN 180

Query: 181 NETSTFTITVDLKNGTSAGVSTADRAKTIVALSSPQSKAEDFRRPGHVFPLRYRDGGVLR 240
           NETSTFTITVD+K GTS GVS ADRAKTIVALSS +SK EDFRRPGHVFP+RYRDGGVLR
Sbjct: 181 NETSTFTITVDVKTGTSGGVSAADRAKTIVALSSSESKPEDFRRPGHVFPVRYRDGGVLR 240

Query: 241 RAGHTEASVDLVSLAGLRSVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRY 300
           RAGHTEASVDLV+LAGLR VSVLS IVD +DGSMASLPKLR LAS+  IP+VSITDLIRY
Sbjct: 241 RAGHTEASVDLVALAGLRPVSVLSAIVDTQDGSMASLPKLRSLASQHQIPMVSITDLIRY 300

Query: 301 RRKRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECL 360
           RRKRENLIERIAISRLPTKWGQFYAHCYRS +DGIEHVALVKGNIGDGQDVLVRVHSECL
Sbjct: 301 RRKRENLIERIAISRLPTKWGQFYAHCYRSKVDGIEHVALVKGNIGDGQDVLVRVHSECL 360

Query: 361 TGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDT 420
           TGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDT
Sbjct: 361 TGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDT 420

Query: 421 VEANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTP 480
           VEAN+ELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVT RVPVVT 
Sbjct: 421 VEANLELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTGRVPVVTQ 480

Query: 481 ITDENRKYLETKRTKMGHIYAKG 495
           ITDENRKYLETKRTKMGHIYAKG
Sbjct: 481 ITDENRKYLETKRTKMGHIYAKG 503

BLAST of CmaCh01G005860 vs. ExPASy TrEMBL
Match: A0A6J1DXS1 (GTP cyclohydrolase II OS=Momordica charantia OX=3673 GN=LOC111024475 PE=3 SV=1)

HSP 1 Score: 727.2 bits (1876), Expect = 4.6e-206
Identity = 391/518 (75.48%), Postives = 427/518 (82.43%), Query Frame = 0

Query: 1   MASALLAHP-LLPRFTNLPLS--SFHRPALPHP-------KIGKCKQRGLRFVC-SAAAD 60
           M   LL HP +LPR+ ++  +  SF       P       KIGKCKQ+GLR+VC  +AA 
Sbjct: 1   MECVLLRHPVMLPRYKSVAPTRWSFSGVVSVAPPVANLTLKIGKCKQKGLRYVCLCSAAG 60

Query: 61  LFDDNL------AAKNGNGAASAAA---LGLEAETTPDVVDFFDDEDDPDCPSEGYSSIP 120
           L   NL      A KN  G  S      LG+  E + +  DF DDEDDPD PS+G+SS+ 
Sbjct: 61  LSAGNLFGESSHARKNNIGHESVGVSTNLGVNPEISFEGDDFVDDEDDPDRPSDGFSSVQ 120

Query: 121 TAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLER 180
           TAVDAI  GKFV+VVDDEN D+EGNLVMAAS  + +NITTMVQ  SG++YV MK DDLER
Sbjct: 121 TAVDAIRHGKFVMVVDDENGDSEGNLVMAASKISATNITTMVQQSSGMVYVAMKGDDLER 180

Query: 181 LKLPIIS-IDNGN-ETSTFTITVDLKNGTSAGVSTADRAKTIVALSSPQSKAEDFRRPGH 240
           L LP+IS +DNGN ETSTFTITVD K  TS GVS ADRAKTIVALSSP+SK EDFRRPGH
Sbjct: 181 LNLPLISLLDNGNYETSTFTITVDAKMETSTGVSAADRAKTIVALSSPESKPEDFRRPGH 240

Query: 241 VFPLRYRDGGVLRRAGHTEASVDLVSLAGLRSVSVLSTIVDPEDGSMASLPKLRKLASEK 300
           VFP+RYRDGGVLRRAGHTEASVDLV+LAGLR VSVLS IVD  DGSMASL KLR LAS+ 
Sbjct: 241 VFPVRYRDGGVLRRAGHTEASVDLVALAGLRPVSVLSPIVDAHDGSMASLSKLRSLASQH 300

Query: 301 NIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGD 360
           +IPIVSITDLIRYRRKRENLIERI++SRLPTKWG+F A+CYRS LDGIEHVA+VKGNI +
Sbjct: 301 DIPIVSITDLIRYRRKRENLIERISVSRLPTKWGKFQAYCYRSKLDGIEHVAIVKGNIAE 360

Query: 361 GQDVLVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPK 420
           GQDVLVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKAGRG VIYLRGHEGRGIGLG K
Sbjct: 361 GQDVLVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKAGRGVVIYLRGHEGRGIGLGAK 420

Query: 421 LLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGY 480
           LLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIG+RTMRLMTNNPAK KGLKGY
Sbjct: 421 LLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIGVRTMRLMTNNPAKLKGLKGY 480

Query: 481 GLAVTARVPVVTPITDENRKYLETKRTKMGHIYAKGQF 497
           GLA+T RVPVVTPITDENRKYLETKRTKMGH+YAK  F
Sbjct: 481 GLAMTGRVPVVTPITDENRKYLETKRTKMGHVYAKSPF 518

BLAST of CmaCh01G005860 vs. ExPASy TrEMBL
Match: A0A7N2KPF3 (GTP cyclohydrolase II OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 2.1e-190
Identity = 357/515 (69.32%), Postives = 409/515 (79.42%), Query Frame = 0

Query: 1   MASALLAHPLLPR-FTNLPLSSFHRPALPHPKIGKC--KQRGLRFVCSAAA-------DL 60
           M SAL  HPL    FT+   SS+H   +   + G C  K R L   C AA        +L
Sbjct: 1   MDSALFTHPLFSHIFTS---SSWHPSLVAFQRAGICLYKHRLLNRPCWAAGVPGIGAKNL 60

Query: 61  FDDNLAAKNGNGA---------ASAAALGLEAETTPDVVDFF--DDEDDPDCPSEGYSSI 120
           FDD+    + NG          ASA    L+A+ TP+ +DFF  D E DPDCPSEGY SI
Sbjct: 61  FDDSSLKGSENGCVLGAFDDELASAPFGTLDAKITPETIDFFVSDAEGDPDCPSEGYFSI 120

Query: 121 PTAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLE 180
             A++ + QGKFVIVVDDEN D EGNLVMAAS T+P ++  MV+HGSGI+ VGMK++DL+
Sbjct: 121 EQALNTLRQGKFVIVVDDENGDIEGNLVMAASLTSPMDVAFMVKHGSGIVSVGMKDEDLQ 180

Query: 181 RLKLPIISIDNGNETS---TFTITVDLKNGTSAGVSTADRAKTIVALSSPQSKAEDFRRP 240
           RLKLP++S +  +E S   TFTITVD K GTS GVS +DRAKT++ALSS +SK EDFRRP
Sbjct: 181 RLKLPLMSPETEDEDSSAPTFTITVDAKTGTSTGVSASDRAKTVLALSSLESKPEDFRRP 240

Query: 241 GHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRSVSVLSTIVDPEDGSMASLPKLRKLAS 300
           GHVFPL+YR+GGVLRRAGHTEASVDLVSLAGLR VSVLS IVD +DGSMASLP LRKLA 
Sbjct: 241 GHVFPLKYRNGGVLRRAGHTEASVDLVSLAGLRPVSVLSAIVDADDGSMASLPGLRKLAL 300

Query: 301 EKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNI 360
           E NIPIVSITDLIRYRRKRENL+ER AISRLPTKWG F A+CYRS LDG EH+A+VKGNI
Sbjct: 301 EHNIPIVSITDLIRYRRKRENLVERTAISRLPTKWGLFQAYCYRSKLDGTEHIAIVKGNI 360

Query: 361 GDGQDVLVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLG 420
           G GQDVLVRVHSECLTGDI  SARCDCGNQLDLAM++IE+AGRG V+YLRGHEGRGIGLG
Sbjct: 361 GHGQDVLVRVHSECLTGDILGSARCDCGNQLDLAMKLIEQAGRGVVVYLRGHEGRGIGLG 420

Query: 421 PKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLK 480
            KL AYNLQDQGHDTV+ANIELGL  DAR+YG+GAQ+LRD+G++TMRLMTNNPAKF GLK
Sbjct: 421 HKLRAYNLQDQGHDTVQANIELGLAVDAREYGIGAQILRDVGVQTMRLMTNNPAKFTGLK 480

Query: 481 GYGLAVTARVPVVTPITDENRKYLETKRTKMGHIY 492
           GYGLAV  RVPV+TPIT+EN+KYLETKRTKMGH+Y
Sbjct: 481 GYGLAVIGRVPVLTPITEENKKYLETKRTKMGHVY 512

BLAST of CmaCh01G005860 vs. NCBI nr
Match: XP_022998776.1 (monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1001.5 bits (2588), Expect = 2.6e-288
Identity = 507/507 (100.00%), Postives = 507/507 (100.00%), Query Frame = 0

Query: 1   MASALLAHPLLPRFTNLPLSSFHRPALPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNG 60
           MASALLAHPLLPRFTNLPLSSFHRPALPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNG
Sbjct: 1   MASALLAHPLLPRFTNLPLSSFHRPALPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNG 60

Query: 61  NGAASAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIPTAVDAISQGKFVIVVDDENE 120
           NGAASAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIPTAVDAISQGKFVIVVDDENE
Sbjct: 61  NGAASAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIPTAVDAISQGKFVIVVDDENE 120

Query: 121 DTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTIT 180
           DTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTIT
Sbjct: 121 DTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTIT 180

Query: 181 VDLKNGTSAGVSTADRAKTIVALSSPQSKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASV 240
           VDLKNGTSAGVSTADRAKTIVALSSPQSKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASV
Sbjct: 181 VDLKNGTSAGVSTADRAKTIVALSSPQSKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASV 240

Query: 241 DLVSLAGLRSVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRENLIE 300
           DLVSLAGLRSVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRENLIE
Sbjct: 241 DLVSLAGLRSVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRENLIE 300

Query: 301 RIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFASAR 360
           RIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFASAR
Sbjct: 301 RIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFASAR 360

Query: 361 CDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGL 420
           CDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGL
Sbjct: 361 CDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGL 420

Query: 421 PADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYL 480
           PADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYL
Sbjct: 421 PADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYL 480

Query: 481 ETKRTKMGHIYAKGQFLRSRQAEASRN 508
           ETKRTKMGHIYAKGQFLRSRQAEASRN
Sbjct: 481 ETKRTKMGHIYAKGQFLRSRQAEASRN 507

BLAST of CmaCh01G005860 vs. NCBI nr
Match: XP_023522983.1 (monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 978.0 bits (2527), Expect = 3.1e-281
Identity = 497/510 (97.45%), Postives = 503/510 (98.63%), Query Frame = 0

Query: 1   MASALLAHPLLPRFTNLPLSSFHRPALPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNG 60
           MASALL+HPLLPRFTNLPLSSF+RPALPHPKIGKCK RGLRFVCSAAADLFDDNLAAKNG
Sbjct: 1   MASALLSHPLLPRFTNLPLSSFNRPALPHPKIGKCKLRGLRFVCSAAADLFDDNLAAKNG 60

Query: 61  NG---AASAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIPTAVDAISQGKFVIVVDD 120
           NG   AA+AAALGLEAETTPDVVDFFDDEDDPDCPS+GYSSI TAVDAISQGKFVIVVDD
Sbjct: 61  NGAAAAAAAAALGLEAETTPDVVDFFDDEDDPDCPSDGYSSIQTAVDAISQGKFVIVVDD 120

Query: 121 ENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTF 180
           ENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTF
Sbjct: 121 ENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTF 180

Query: 181 TITVDLKNGTSAGVSTADRAKTIVALSSPQSKAEDFRRPGHVFPLRYRDGGVLRRAGHTE 240
           TITVDLKNGTSAGVSTADRAKTIVALSSP+SKAEDFRRPGHVFPLRYRDGGVLRRAGHTE
Sbjct: 181 TITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTE 240

Query: 241 ASVDLVSLAGLRSVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKREN 300
           ASVDLVSLAGLR VSVLSTIVDPEDGSMASLPK+RKLASEKNIPIVSITDLIRYRRKREN
Sbjct: 241 ASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKMRKLASEKNIPIVSITDLIRYRRKREN 300

Query: 301 LIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFA 360
           LIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFA
Sbjct: 301 LIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFA 360

Query: 361 SARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIE 420
           SARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIE
Sbjct: 361 SARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIE 420

Query: 421 LGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENR 480
           LGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENR
Sbjct: 421 LGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENR 480

Query: 481 KYLETKRTKMGHIYAKGQFLRSRQAEASRN 508
           KYLETKRTKMGHIYAKGQFLRSRQAEAS N
Sbjct: 481 KYLETKRTKMGHIYAKGQFLRSRQAEASTN 510

BLAST of CmaCh01G005860 vs. NCBI nr
Match: XP_022948312.1 (monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 976.1 bits (2522), Expect = 1.2e-280
Identity = 497/511 (97.26%), Postives = 501/511 (98.04%), Query Frame = 0

Query: 1   MASALLAHPLLPRFTNLPLSSFHRPALPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNG 60
           MAS LL+HPLLPRFTNLPLSSF+RPALPHPKIGKCK RGLRFVCSAAADLFDDNLAAKNG
Sbjct: 1   MASTLLSHPLLPRFTNLPLSSFNRPALPHPKIGKCKLRGLRFVCSAAADLFDDNLAAKNG 60

Query: 61  NG----AASAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIPTAVDAISQGKFVIVVD 120
           NG    AA+AAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSI  AVDAISQGKFVIVVD
Sbjct: 61  NGAAAAAAAAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIQNAVDAISQGKFVIVVD 120

Query: 121 DENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETST 180
           DENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETST
Sbjct: 121 DENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETST 180

Query: 181 FTITVDLKNGTSAGVSTADRAKTIVALSSPQSKAEDFRRPGHVFPLRYRDGGVLRRAGHT 240
           FTITVDLKNGTSAGVSTADRAKTIVALSSP+SKAEDFRRPGHVFPLRYRDGGVLRRAGHT
Sbjct: 181 FTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHT 240

Query: 241 EASVDLVSLAGLRSVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRE 300
           EASVDLVSLAGLR VSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRE
Sbjct: 241 EASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRE 300

Query: 301 NLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIF 360
           NLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIF
Sbjct: 301 NLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIF 360

Query: 361 ASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANI 420
           ASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANI
Sbjct: 361 ASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANI 420

Query: 421 ELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDEN 480
           ELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDEN
Sbjct: 421 ELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDEN 480

Query: 481 RKYLETKRTKMGHIYAKGQFLRSRQAEASRN 508
           RKYLETKRTKMGHIYAKGQFLRSRQAEAS N
Sbjct: 481 RKYLETKRTKMGHIYAKGQFLRSRQAEASTN 511

BLAST of CmaCh01G005860 vs. NCBI nr
Match: KAG6607228.1 (Monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 972.2 bits (2512), Expect = 1.7e-279
Identity = 495/512 (96.68%), Postives = 501/512 (97.85%), Query Frame = 0

Query: 1   MASALLAHPLLPRFTNLPLSSFHRPALPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNG 60
           MAS LL+HPLLPRFTNLPLSSF+R +LPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNG
Sbjct: 1   MASTLLSHPLLPRFTNLPLSSFNRLSLPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNG 60

Query: 61  NG-----AASAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIPTAVDAISQGKFVIVV 120
           NG     AA+AAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSI  AVDAISQGKFVIVV
Sbjct: 61  NGAAAAAAAAAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIQNAVDAISQGKFVIVV 120

Query: 121 DDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETS 180
           DDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETS
Sbjct: 121 DDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETS 180

Query: 181 TFTITVDLKNGTSAGVSTADRAKTIVALSSPQSKAEDFRRPGHVFPLRYRDGGVLRRAGH 240
           TFTITVDLKNGTSAGVSTADRAKTIVALSSP+SKAEDFRRPGHVFPLRYRDGGVLRRAGH
Sbjct: 181 TFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGH 240

Query: 241 TEASVDLVSLAGLRSVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKR 300
           TEASVDLVSLAGLR VSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKR
Sbjct: 241 TEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKR 300

Query: 301 ENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDI 360
           ENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDI
Sbjct: 301 ENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDI 360

Query: 361 FASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEAN 420
           FASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEAN
Sbjct: 361 FASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEAN 420

Query: 421 IELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDE 480
           IELGLPADARDYGVGAQMLRDIGIRTMRLMTNNP+KFKGLKGYGLAVTARVPVVTPITDE
Sbjct: 421 IELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPSKFKGLKGYGLAVTARVPVVTPITDE 480

Query: 481 NRKYLETKRTKMGHIYAKGQFLRSRQAEASRN 508
           NRKYLETKRTKMGHIYAKGQFLRSRQAEAS N
Sbjct: 481 NRKYLETKRTKMGHIYAKGQFLRSRQAEASTN 512

BLAST of CmaCh01G005860 vs. NCBI nr
Match: XP_022159223.1 (monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic-like [Momordica charantia])

HSP 1 Score: 795.8 bits (2054), Expect = 2.2e-226
Identity = 410/503 (81.51%), Postives = 443/503 (88.07%), Query Frame = 0

Query: 1   MASALLAHPLLPRFTNLPLSSF-HRPAL---PHPKIGKCKQRGLRFVCSAAA----DLFD 60
           M SALL HPL  RF NL LS+F +RP+    P   + K KQR      SAAA    ++FD
Sbjct: 1   MDSALLCHPLFTRFNNLTLSTFNNRPSFAPPPPQTLRKSKQRTSNSASSAAAFSAGNMFD 60

Query: 61  DNLAAKNGNGAASAAALGLEAETTPDVVDFFDDEDDPDCPSEGYSSIPTAVDAISQGKFV 120
           +    KNG  + +A+ LGL AET+ + +DFFD+ +DPD PS+GYSSI +AVDAI QGKFV
Sbjct: 61  ETPTTKNGKPSVAASTLGLGAETSSEAIDFFDNLEDPDHPSDGYSSIQSAVDAIHQGKFV 120

Query: 121 IVVDDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNG- 180
           IVVDDEN D+EGNLVMAASF TP N+T+MVQHGSGI+YVGMKEDDL+RLKLP+ S+DNG 
Sbjct: 121 IVVDDENGDSEGNLVMAASFATPGNVTSMVQHGSGIVYVGMKEDDLQRLKLPLFSVDNGN 180

Query: 181 NETSTFTITVDLKNGTSAGVSTADRAKTIVALSSPQSKAEDFRRPGHVFPLRYRDGGVLR 240
           NETSTFTITVD+K GTS GVS ADRAKTIVALSS +SK EDFRRPGHVFP+RYRDGGVLR
Sbjct: 181 NETSTFTITVDVKTGTSGGVSAADRAKTIVALSSSESKPEDFRRPGHVFPVRYRDGGVLR 240

Query: 241 RAGHTEASVDLVSLAGLRSVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRY 300
           RAGHTEASVDLV+LAGLR VSVLS IVD +DGSMASLPKLR LAS+  IP+VSITDLIRY
Sbjct: 241 RAGHTEASVDLVALAGLRPVSVLSAIVDTQDGSMASLPKLRSLASQHQIPMVSITDLIRY 300

Query: 301 RRKRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECL 360
           RRKRENLIERIAISRLPTKWGQFYAHCYRS +DGIEHVALVKGNIGDGQDVLVRVHSECL
Sbjct: 301 RRKRENLIERIAISRLPTKWGQFYAHCYRSKVDGIEHVALVKGNIGDGQDVLVRVHSECL 360

Query: 361 TGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDT 420
           TGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDT
Sbjct: 361 TGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDT 420

Query: 421 VEANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTP 480
           VEAN+ELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVT RVPVVT 
Sbjct: 421 VEANLELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTGRVPVVTQ 480

Query: 481 ITDENRKYLETKRTKMGHIYAKG 495
           ITDENRKYLETKRTKMGHIYAKG
Sbjct: 481 ITDENRKYLETKRTKMGHIYAKG 503

BLAST of CmaCh01G005860 vs. TAIR 10
Match: AT5G59750.1 (DHBP synthase RibB-like alpha/beta domain;GTP cyclohydrolase II )

HSP 1 Score: 630.6 bits (1625), Expect = 1.1e-180
Identity = 313/449 (69.71%), Postives = 380/449 (84.63%), Query Frame = 0

Query: 49  DLFDDNLAAK-NGNGAASAAALGLEAETTPDVVDFF--DDEDDPDCPSEGYSSIPTAVDA 108
           ++FD+N   K +G+   SA+   ++AE TP+  DFF  D E DPDCP++GYSSI  A+ A
Sbjct: 51  NVFDENPVRKTDGSLFDSASFETVDAEITPETDDFFVSDAEGDPDCPTQGYSSIELALQA 110

Query: 109 ISQGKFVIVVDDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPI 168
           + +GKFVIVVDDE  D EGNL+MAA+ T+P +I  ++++GSGI+ VGMK+++LERL L +
Sbjct: 111 LRKGKFVIVVDDETGDVEGNLIMAATLTSPKDIAFLIKNGSGIVSVGMKKENLERLSLTL 170

Query: 169 ISIDNGNETS---TFTITVDLKNGTSAGVSTADRAKTIVALSSPQSKAEDFRRPGHVFPL 228
           +S +  +E S   TFTITVD K+GTS GVS +DRA T++ALSS  +K +DFRRPGHVFPL
Sbjct: 171 MSPEMEDEDSSAPTFTITVDAKSGTSTGVSASDRAMTVLALSSLDAKPDDFRRPGHVFPL 230

Query: 229 RYRDGGVLRRAGHTEASVDLVSLAGLRSVSVLSTIVDPEDGSMASLPKLRKLASEKNIPI 288
           +YRDGGVLRRAGHTEASVDL+ LAGLR +SVLS I+D EDGSMASLP ++KLA+E +IPI
Sbjct: 231 KYRDGGVLRRAGHTEASVDLMILAGLRPLSVLSAILDQEDGSMASLPYMKKLATEHDIPI 290

Query: 289 VSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDV 348
           VS+TDLIRYRRKR+ L+ERI +SRLPTKWG F A+CYRS LDG E++ALVKGN+G+G+D+
Sbjct: 291 VSLTDLIRYRRKRDKLVERITVSRLPTKWGLFQAYCYRSKLDGTENIALVKGNVGNGEDI 350

Query: 349 LVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAY 408
           LVRVHSECLTGDIF SARCDCGNQLDLAM++IEK GRG V+YLRGHEGRGIGLG KL AY
Sbjct: 351 LVRVHSECLTGDIFGSARCDCGNQLDLAMELIEKEGRGVVVYLRGHEGRGIGLGHKLRAY 410

Query: 409 NLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAV 468
           NLQD+GHDTV+AN+ELGL  D+R+YG+GAQMLRDIG+RTMRLMTNNPAKF GLKGYGLAV
Sbjct: 411 NLQDEGHDTVQANVELGLSIDSREYGIGAQMLRDIGVRTMRLMTNNPAKFTGLKGYGLAV 470

Query: 469 TARVPVVTPITDENRKYLETKRTKMGHIY 492
             RVPVVTPIT ENR+Y+ETKR KMGHIY
Sbjct: 471 VGRVPVVTPITKENRRYMETKRKKMGHIY 499

BLAST of CmaCh01G005860 vs. TAIR 10
Match: AT5G59750.2 (DHBP synthase RibB-like alpha/beta domain;GTP cyclohydrolase II )

HSP 1 Score: 613.2 bits (1580), Expect = 1.9e-175
Identity = 313/483 (64.80%), Postives = 380/483 (78.67%), Query Frame = 0

Query: 49  DLFDDNLAAK-NGNGAASAAALGLEAETTPDVVDFF--DDEDDPDCPSEGYSSIPTAVDA 108
           ++FD+N   K +G+   SA+   ++AE TP+  DFF  D E DPDCP++GYSSI  A+ A
Sbjct: 51  NVFDENPVRKTDGSLFDSASFETVDAEITPETDDFFVSDAEGDPDCPTQGYSSIELALQA 110

Query: 109 ISQGK----------------------------------FVIVVDDENEDTEGNLVMAAS 168
           + +GK                                  FVIVVDDE  D EGNL+MAA+
Sbjct: 111 LRKGKVCSLTIFLKTSDYDDLFLQLYRFGLTKKLSLMFQFVIVVDDETGDVEGNLIMAAT 170

Query: 169 FTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETS---TFTITVDLKNGTS 228
            T+P +I  ++++GSGI+ VGMK+++LERL L ++S +  +E S   TFTITVD K+GTS
Sbjct: 171 LTSPKDIAFLIKNGSGIVSVGMKKENLERLSLTLMSPEMEDEDSSAPTFTITVDAKSGTS 230

Query: 229 AGVSTADRAKTIVALSSPQSKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGL 288
            GVS +DRA T++ALSS  +K +DFRRPGHVFPL+YRDGGVLRRAGHTEASVDL+ LAGL
Sbjct: 231 TGVSASDRAMTVLALSSLDAKPDDFRRPGHVFPLKYRDGGVLRRAGHTEASVDLMILAGL 290

Query: 289 RSVSVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLP 348
           R +SVLS I+D EDGSMASLP ++KLA+E +IPIVS+TDLIRYRRKR+ L+ERI +SRLP
Sbjct: 291 RPLSVLSAILDQEDGSMASLPYMKKLATEHDIPIVSLTDLIRYRRKRDKLVERITVSRLP 350

Query: 349 TKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFASARCDCGNQLD 408
           TKWG F A+CYRS LDG E++ALVKGN+G+G+D+LVRVHSECLTGDIF SARCDCGNQLD
Sbjct: 351 TKWGLFQAYCYRSKLDGTENIALVKGNVGNGEDILVRVHSECLTGDIFGSARCDCGNQLD 410

Query: 409 LAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYG 468
           LAM++IEK GRG V+YLRGHEGRGIGLG KL AYNLQD+GHDTV+AN+ELGL  D+R+YG
Sbjct: 411 LAMELIEKEGRGVVVYLRGHEGRGIGLGHKLRAYNLQDEGHDTVQANVELGLSIDSREYG 470

Query: 469 VGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMG 492
           +GAQMLRDIG+RTMRLMTNNPAKF GLKGYGLAV  RVPVVTPIT ENR+Y+ETKR KMG
Sbjct: 471 IGAQMLRDIGVRTMRLMTNNPAKFTGLKGYGLAVVGRVPVVTPITKENRRYMETKRKKMG 530

BLAST of CmaCh01G005860 vs. TAIR 10
Match: AT5G64300.1 (GTP cyclohydrolase II )

HSP 1 Score: 565.8 bits (1457), Expect = 3.4e-161
Identity = 301/527 (57.12%), Postives = 376/527 (71.35%), Query Frame = 0

Query: 6   LAHPLLPRFTNLPLSSFHRPALP--HPKIGKCKQRGLRFVCSAAADLFDDNLAAKNGNG- 65
           L+   L + +   L   HR  LP  HP      +     V +A     DD L+  NGN  
Sbjct: 16  LSRSRLSQSSTTLLHGLHRVTLPSNHPLSTFSIKTNTGKVKAAVISREDDLLSFTNGNTP 75

Query: 66  ---------------AASAAALG-LEAETTPDVVDFFDDEDDP---DCPSEGYSSIPTAV 125
                           A + +LG L A++ P   + F  EDD    D P+ G+SSIP A+
Sbjct: 76  LSNGSLIDDRTEEPLEADSVSLGTLAADSAPAPANGFVAEDDDFELDLPTPGFSSIPEAI 135

Query: 126 DAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKL 185
           + I QGK V+VVDDE+ + EG+LVMAA   TP  +  +V+HG+GI+ V MKEDDLERL L
Sbjct: 136 EDIRQGKLVVVVDDEDRENEGDLVMAAQLATPEAMAFIVRHGTGIVCVSMKEDDLERLHL 195

Query: 186 PIISIDNGNE---TSTFTITVDLKNGTSAGVSTADRAKTIVALSSPQSKAEDFRRPGHVF 245
           P++     NE   ++ FT+TVD K+GT+ GVS  DRA TI++L+S  SK EDF RPGH+F
Sbjct: 196 PLMVNQKENEEKLSTAFTVTVDAKHGTTTGVSARDRATTILSLASRDSKPEDFNRPGHIF 255

Query: 246 PLRYRDGGVLRRAGHTEASVDLVSLAGLRSVSVLSTIVDPEDGSMASLPKLRKLASEKNI 305
           PL+YR+GGVL+RAGHTEASVDL  LAGL  V VL  IVD +DGSMA LPKLR+ A+E N+
Sbjct: 256 PLKYREGGVLKRAGHTEASVDLTVLAGLDPVGVLCEIVD-DDGSMARLPKLREFAAENNL 315

Query: 306 PIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKGNIGDGQ 365
            +VSI DLIRYRRKR+ L+ER + +R+PT WG F A+CYRS LDGIEH+A+VKG IGDGQ
Sbjct: 316 KVVSIADLIRYRRKRDKLVERASAARIPTMWGPFTAYCYRSILDGIEHIAMVKGEIGDGQ 375

Query: 366 DVLVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKAGRGAVIYLRGHEGRGIGLGPKLL 425
           D+LVRVHSECLTGDIF SARCDCGNQL L+MQ IE  GRG ++YLRGHEGRGIGLG KL 
Sbjct: 376 DILVRVHSECLTGDIFGSARCDCGNQLALSMQQIEATGRGVLVYLRGHEGRGIGLGHKLR 435

Query: 426 AYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGL 485
           AYNLQD G DTVEAN ELGLP D+R+YG+GAQ++RD+G+RTM+LMTNNPAK+ GLKGYGL
Sbjct: 436 AYNLQDAGRDTVEANEELGLPVDSREYGIGAQIIRDLGVRTMKLMTNNPAKYVGLKGYGL 495

Query: 486 AVTARVPVVTPITDENRKYLETKRTKMGHIYA---KGQFLRSRQAEA 505
           A+  RVP+++ IT EN++YLETKRTKMGH+Y    KG  +   ++E+
Sbjct: 496 AIVGRVPLLSLITKENKRYLETKRTKMGHMYGLKFKGDVVEKIESES 541

BLAST of CmaCh01G005860 vs. TAIR 10
Match: AT2G22450.1 (riboflavin biosynthesis protein, putative )

HSP 1 Score: 438.3 bits (1126), Expect = 8.2e-123
Identity = 220/399 (55.14%), Postives = 294/399 (73.68%), Query Frame = 0

Query: 75  TTPDVVDFFDDEDDPDCPSEGYSSIPTAVDAISQGKFVIVVDDENEDTEGNLVMAASFTT 134
           TT D  +  D+E D D P++G++SIP A++ I  GK V+VVDDE+ + EG+L+MAAS  T
Sbjct: 85  TTMDSPEVEDEEFDLDRPTDGFASIPQAIEDIRHGKMVVVVDDEDRENEGDLIMAASLAT 144

Query: 135 PSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNE---TSTFTITVDLKNGTSAGV 194
           P  +  +V+HG+GI+ V MK +DLERL+LP++     NE    + FT++VD K GTS GV
Sbjct: 145 PEAMAFVVKHGTGIVCVSMKGEDLERLELPLMVTRKDNEEKLRTAFTVSVDAKKGTSTGV 204

Query: 195 STADRAKTIVALSSPQSKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRSV 254
           S  DRA+TI+ L+S  SK EDF RPGH+FPLRYR+GGVL+RAGHTEASVDL  LAGL  V
Sbjct: 205 SARDRAQTILTLASKDSKPEDFNRPGHIFPLRYREGGVLKRAGHTEASVDLTVLAGLEPV 264

Query: 255 SVLSTIVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKW 314
           SVL  IVD +DGSMA LP+LR+ A E N+ ++SI DLIRYRRKRE L+E  A++ +PT W
Sbjct: 265 SVLCEIVD-DDGSMARLPRLRQFAQENNLKLISIADLIRYRRKRERLVEFTAVAPIPTMW 324

Query: 315 GQFYAHCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFASARCDCGNQLDLAM 374
           G F AHC++S LDG+EH+A+VKG IGDG+D+LVRVH+EC+T DIF ++    G QL +AM
Sbjct: 325 GPFKAHCFKSLLDGVEHIAMVKGEIGDGKDILVRVHAECITDDIFGNS--SGGKQLAIAM 384

Query: 375 QMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGA 434
           ++IE+ GRG  +YLRG E +GI L  K   YN      D  E    +  P  +R+YG+GA
Sbjct: 385 RLIEENGRGVFVYLRGPESKGIDLSHKPRTYNTNS---DQAEG---VSFPVASREYGIGA 444

Query: 435 QMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVT 471
           Q+LRD+G+R M++MTNNPA + GLKGYGL+++ +VP++T
Sbjct: 445 QILRDLGVREMKVMTNNPAHYVGLKGYGLSISGKVPLIT 474

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FN891.6e-17969.71Monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic OS=Arabidop... [more]
Q6L5061.4e-16766.67Probable monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic OS... [more]
Q6Z2341.4e-16465.44Probable bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic OS=O... [more]
Q0E0795.6e-16166.04Probable bifunctional riboflavin biosynthesis protein RIBA 2, chloroplastic OS=O... [more]
P479244.8e-16057.12Bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic OS=Arabidopsi... [more]
Match NameE-valueIdentityDescription
A0A6J1KB551.3e-288100.00GTP cyclohydrolase II OS=Cucurbita maxima OX=3661 GN=LOC111493343 PE=3 SV=1[more]
A0A6J1G8U45.6e-28197.26GTP cyclohydrolase II OS=Cucurbita moschata OX=3662 GN=LOC111452026 PE=3 SV=1[more]
A0A6J1DY851.0e-22681.51GTP cyclohydrolase II OS=Momordica charantia OX=3673 GN=LOC111025642 PE=3 SV=1[more]
A0A6J1DXS14.6e-20675.48GTP cyclohydrolase II OS=Momordica charantia OX=3673 GN=LOC111024475 PE=3 SV=1[more]
A0A7N2KPF32.1e-19069.32GTP cyclohydrolase II OS=Quercus lobata OX=97700 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022998776.12.6e-288100.00monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic-like [Cucur... [more]
XP_023522983.13.1e-28197.45monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic-like [Cucur... [more]
XP_022948312.11.2e-28097.26monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic-like [Cucur... [more]
KAG6607228.11.7e-27996.68Monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic, partial [C... [more]
XP_022159223.12.2e-22681.51monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic-like [Momor... [more]
Match NameE-valueIdentityDescription
AT5G59750.11.1e-18069.71DHBP synthase RibB-like alpha/beta domain;GTP cyclohydrolase II [more]
AT5G59750.21.9e-17564.80DHBP synthase RibB-like alpha/beta domain;GTP cyclohydrolase II [more]
AT5G64300.13.4e-16157.12GTP cyclohydrolase II [more]
AT2G22450.18.2e-12355.14riboflavin biosynthesis protein, putative [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0004223,4-dihydroxy-2-butanone 4-phosphate synthase, RibBTIGRFAMTIGR00506TIGR00506coord: 98..292
e-value: 8.1E-56
score: 186.7
IPR0004223,4-dihydroxy-2-butanone 4-phosphate synthase, RibBPFAMPF00926DHBP_synthasecoord: 101..291
e-value: 1.3E-67
score: 227.0
IPR032677GTP cyclohydrolase IIPFAMPF00925GTP_cyclohydro2coord: 304..467
e-value: 3.0E-71
score: 238.3
IPR000926GTP cyclohydrolase II, RibATIGRFAMTIGR00505TIGR00505coord: 305..490
e-value: 1.2E-74
score: 248.0
IPR000926GTP cyclohydrolase II, RibAHAMAPMF_00179RibAcoord: 297..493
score: 40.06826
IPR000926GTP cyclohydrolase II, RibACDDcd00641GTP_cyclohydro2coord: 298..490
e-value: 4.55487E-117
score: 341.017
NoneNo IPR availableGENE3D3.90.870.10DHBP synthasecoord: 89..298
e-value: 1.6E-74
score: 251.6
NoneNo IPR availablePANTHERPTHR21327GTP CYCLOHYDROLASE II-RELATEDcoord: 59..492
NoneNo IPR availablePANTHERPTHR21327:SF29MONOFUNCTIONAL RIBOFLAVIN BIOSYNTHESIS PROTEIN RIBA 3, CHLOROPLASTICcoord: 59..492
IPR036144GTP cyclohydrolase II superfamilyGENE3D3.40.50.10990GTP cyclohydrolase IIcoord: 299..470
e-value: 8.6E-77
score: 258.3
IPR036144GTP cyclohydrolase II superfamilySUPERFAMILY142695RibA-likecoord: 298..469
IPR016299Riboflavin biosynthesis protein RibBAHAMAPMF_01283RibBAcoord: 95..489
score: 48.864727
IPR017945DHBP synthase RibB-like alpha/beta domain superfamilySUPERFAMILY55821YrdC/RibBcoord: 96..296

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G005860.1CmaCh01G005860.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009231 riboflavin biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0008686 3,4-dihydroxy-2-butanone-4-phosphate synthase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0003935 GTP cyclohydrolase II activity
molecular_function GO:0046872 metal ion binding