CmaCh01G004400 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh01G004400
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionalpha/beta-Hydrolases superfamily protein
LocationCma_Chr01: 2210879 .. 2211913 (-)
RNA-Seq ExpressionCmaCh01G004400
SyntenyCmaCh01G004400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCCATGTACATCGATCCAAGGCTCAAACTCCAGATGGCCAAACCCCACCTTCAAAATGGAGCCACGTCAGAAGAGATTGAAGGGCTGATTCGAGTTTATGCAGATGGGCATGTTGAAAGGCCCCCAATCATCCCAAATGTAGCCTGCAGCACCACAGCATCAAACAAAGGCGTAACTGCAAAAGATGTTCTCATAGACATTTTCACAAACACTTGGGCGCGTATTTACATTCCTAACTCTCAGCGAAACCTCCCTTTGCTTGTTTACTTCCATGGCGGTGGCTTTTGCGTTGGCTCTGCCGCCTGGAGCTGTTACCATGAATTCCTCCTCAATCTTGCTTCCAAAGCAAAATGTGTAATCCTCTCAATCAACTATCGTCTTTCCCCTGAAAATCGCCTCCCCGCCGCTTATGAAGACGGATTCAACGCAGTCATGTGGTTGAAACAACAAGCAATAACCAACCCAATCGAACACAAATGGTGGTTAGGCAATTGCAATCCATCAAAATTGTTCCTCGCGGGCGATAGCGCTGGAGCAAACATAGCATACAACACCGCTACAAGGCTAGGGCACAACGAATCCACACTCCTAAAACCCCCAACTCTAAAGGGTATAATCCTAATCCAACCTTTCTTCGGCGGAGAATCGCGAACCAAATCAGAAAAGCTCGCCGCTACGCCCTCCTCCACCTCCGCGCTAACTCTCTCCGCCTCCGACACATACTGGCGGCTGTCCCTTCCGCCGACGGCCAATCGCGACCATCCATGGAGCAACCCACTGGTGAATGGCGGCGCAAAGCTGAAAGAAATGAAACTCCCACCATTAATGGTATGCATATCGGAGTTGGACATACTGTTGGACAGAAACTTAGAATTCTGTGCAGCCATGGCAGCCGCCGGGAAGAAAGTGGAGAGAGTGATTCTGAAGGGAGTTGGACATGGCTTCCAAATTCTGAACAAAGATCAGATCTCCACTCAGCGAACACAAGAACTAATGGGTTCCATTCGAGCTTTCATTCATCAACAATAG

mRNA sequence

ATGGCTGCCATGTACATCGATCCAAGGCTCAAACTCCAGATGGCCAAACCCCACCTTCAAAATGGAGCCACGTCAGAAGAGATTGAAGGGCTGATTCGAGTTTATGCAGATGGGCATGTTGAAAGGCCCCCAATCATCCCAAATGTAGCCTGCAGCACCACAGCATCAAACAAAGGCGTAACTGCAAAAGATGTTCTCATAGACATTTTCACAAACACTTGGGCGCGTATTTACATTCCTAACTCTCAGCGAAACCTCCCTTTGCTTGTTTACTTCCATGGCGGTGGCTTTTGCGTTGGCTCTGCCGCCTGGAGCTGTTACCATGAATTCCTCCTCAATCTTGCTTCCAAAGCAAAATGTGTAATCCTCTCAATCAACTATCGTCTTTCCCCTGAAAATCGCCTCCCCGCCGCTTATGAAGACGGATTCAACGCAGTCATGTGGTTGAAACAACAAGCAATAACCAACCCAATCGAACACAAATGGTGGTTAGGCAATTGCAATCCATCAAAATTGTTCCTCGCGGGCGATAGCGCTGGAGCAAACATAGCATACAACACCGCTACAAGGCTAGGGCACAACGAATCCACACTCCTAAAACCCCCAACTCTAAAGGGTATAATCCTAATCCAACCTTTCTTCGGCGGAGAATCGCGAACCAAATCAGAAAAGCTCGCCGCTACGCCCTCCTCCACCTCCGCGCTAACTCTCTCCGCCTCCGACACATACTGGCGGCTGTCCCTTCCGCCGACGGCCAATCGCGACCATCCATGGAGCAACCCACTGGTGAATGGCGGCGCAAAGCTGAAAGAAATGAAACTCCCACCATTAATGGTATGCATATCGGAGTTGGACATACTGTTGGACAGAAACTTAGAATTCTGTGCAGCCATGGCAGCCGCCGGGAAGAAAGTGGAGAGAGTGATTCTGAAGGGAGTTGGACATGGCTTCCAAATTCTGAACAAAGATCAGATCTCCACTCAGCGAACACAAGAACTAATGGGTTCCATTCGAGCTTTCATTCATCAACAATAG

Coding sequence (CDS)

ATGGCTGCCATGTACATCGATCCAAGGCTCAAACTCCAGATGGCCAAACCCCACCTTCAAAATGGAGCCACGTCAGAAGAGATTGAAGGGCTGATTCGAGTTTATGCAGATGGGCATGTTGAAAGGCCCCCAATCATCCCAAATGTAGCCTGCAGCACCACAGCATCAAACAAAGGCGTAACTGCAAAAGATGTTCTCATAGACATTTTCACAAACACTTGGGCGCGTATTTACATTCCTAACTCTCAGCGAAACCTCCCTTTGCTTGTTTACTTCCATGGCGGTGGCTTTTGCGTTGGCTCTGCCGCCTGGAGCTGTTACCATGAATTCCTCCTCAATCTTGCTTCCAAAGCAAAATGTGTAATCCTCTCAATCAACTATCGTCTTTCCCCTGAAAATCGCCTCCCCGCCGCTTATGAAGACGGATTCAACGCAGTCATGTGGTTGAAACAACAAGCAATAACCAACCCAATCGAACACAAATGGTGGTTAGGCAATTGCAATCCATCAAAATTGTTCCTCGCGGGCGATAGCGCTGGAGCAAACATAGCATACAACACCGCTACAAGGCTAGGGCACAACGAATCCACACTCCTAAAACCCCCAACTCTAAAGGGTATAATCCTAATCCAACCTTTCTTCGGCGGAGAATCGCGAACCAAATCAGAAAAGCTCGCCGCTACGCCCTCCTCCACCTCCGCGCTAACTCTCTCCGCCTCCGACACATACTGGCGGCTGTCCCTTCCGCCGACGGCCAATCGCGACCATCCATGGAGCAACCCACTGGTGAATGGCGGCGCAAAGCTGAAAGAAATGAAACTCCCACCATTAATGGTATGCATATCGGAGTTGGACATACTGTTGGACAGAAACTTAGAATTCTGTGCAGCCATGGCAGCCGCCGGGAAGAAAGTGGAGAGAGTGATTCTGAAGGGAGTTGGACATGGCTTCCAAATTCTGAACAAAGATCAGATCTCCACTCAGCGAACACAAGAACTAATGGGTTCCATTCGAGCTTTCATTCATCAACAATAG

Protein sequence

MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGVTAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAGANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSASDTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAAAGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ
Homology
BLAST of CmaCh01G004400 vs. ExPASy Swiss-Prot
Match: Q9LFR7 (Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 9.0e-94
Identity = 183/333 (54.95%), Postives = 228/333 (68.47%), Query Frame = 0

Query: 18  HLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGVTAKDVLIDIFTNTWARI 77
           H ++G   EEIEGLI+V+ DG VERPPI+P V+ +   S+K  TA D  I +  +TW R+
Sbjct: 22  HHRHGPVVEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSK-ATAFD--IKLSNDTWTRV 81

Query: 78  YIPNSQR-----NLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPE 137
           YIP++        LPLLVYFHGGGFCVGSAAWSCYH+FL +LA KA+CVI+S+NYRL+PE
Sbjct: 82  YIPDAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPE 141

Query: 138 NRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAGANIAYNTATRL- 197
           +RLPAAY+DG N V WL +Q I+    +  WL  CN S +FLAGDSAGANIAY  A R+ 
Sbjct: 142 HRLPAAYDDGVNVVSWLVKQQISTGGGYPSWLSKCNLSNVFLAGDSAGANIAYQVAVRIM 201

Query: 198 --GHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSASDTYWRLSLP 257
             G   +TL     LKGIILI PFFGGESRT SEK     + +SALTLSASD YWRL+LP
Sbjct: 202 ASGKYANTL----HLKGIILIHPFFGGESRTSSEK-QQHHTKSSALTLSASDAYWRLALP 261

Query: 258 PTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAAAGKKVERVI 317
             A+RDHPW NPL++        KLP  MV ++E DIL +RNLE C  M + GK+VE ++
Sbjct: 262 RGASRDHPWCNPLMSSAG----AKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIV 321

Query: 318 LKGVGHGFQILNKDQISTQRTQELMGSIRAFIH 343
             GVGH F IL+   +S  R  ++M  +  FIH
Sbjct: 322 HGGVGHAFHILDNSSVSRDRIHDMMCRLHNFIH 342

BLAST of CmaCh01G004400 vs. ExPASy Swiss-Prot
Match: Q9SX25 (Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 1.6e-82
Identity = 156/327 (47.71%), Postives = 213/327 (65.14%), Query Frame = 0

Query: 21  NGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGVTAKDVLIDIFTNTWARIYIP 80
           +G   +E+EGLI+VY DGHVER  ++P V  S      GVT  DV+ID  TN WAR+Y+P
Sbjct: 20  HGPVVDEVEGLIKVYKDGHVERSQLLPCVDPSLPL-ELGVTCSDVVIDKLTNVWARLYVP 79

Query: 81  -----NSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPENRL 140
                +S   LPL+VYFHGGGFCVGSA+W CYHEFL  L+++++C+++S+NYRL+PEN L
Sbjct: 80  MTTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPL 139

Query: 141 PAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAGANIAYNTATRLGHNE 200
           PAAYEDG NA++WL +    N      W   C+  ++FLAGDSAG NIA   A RL   E
Sbjct: 140 PAAYEDGVNAILWLNKARNDN-----LWAKQCDFGRIFLAGDSAGGNIAQQVAARLASPE 199

Query: 201 STLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSASDTYWRLSLPPTANRD 260
              LK   ++G ILIQPF+ GE RT+SE+       T+ LTL++SD +WR+SLP  ANR+
Sbjct: 200 DLALK---IEGTILIQPFYSGEERTESERRVGN-DKTAVLTLASSDAWWRMSLPRGANRE 259

Query: 261 HPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAAAGKKVERVILKGVGH 320
           HP+  P+      +K   +   +VC++E+D+L+D N+E C         ++RV+ KGVGH
Sbjct: 260 HPYCKPV---KMIIKSSTVTRTLVCVAEMDLLMDSNMEMCDGNEDV---IKRVLHKGVGH 319

Query: 321 GFQILNKDQISTQRTQELMGSIRAFIH 343
            F IL K Q++   T E++  I AFIH
Sbjct: 320 AFHILGKSQLAHTTTLEMLCQIDAFIH 330

BLAST of CmaCh01G004400 vs. ExPASy Swiss-Prot
Match: Q9FG13 (Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 9.7e-48
Identity = 111/299 (37.12%), Postives = 170/299 (56.86%), Query Frame = 0

Query: 25  SEEIEGLIRVYADGHVERPPIIPNVACSTT-ASNKGVTAKDVLIDIFTNTWARIYIPNSQ 84
           +E+  GL+++ ++G V R   I  +       +N+ V  KD +     N   R+Y P S 
Sbjct: 11  AEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPISA 70

Query: 85  RN---LPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPENRLPAAYE 144
            N   LP++V+FHGGGFC GS +W  +H F L LAS    +++S +YRL+PE+RLPAA+E
Sbjct: 71  SNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFE 130

Query: 145 DGFNAVMWLKQQAITNPIEHKWWLG-NCNPSKLFLAGDSAGANIAYNTATRLGHNESTLL 204
           D    + WL  QA+++ + H +  G + +  ++F+ GDS+G NIA+  A R G + S  L
Sbjct: 131 DAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-SGSIEL 190

Query: 205 KPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSASDTYWRLSLPPTANRDHPWS 264
            P  ++G +L+ PFFGGE RT SE       S + L+L   D +WRLSLP  A RDH  +
Sbjct: 191 TPVRVRGYVLMGPFFGGEERTNSE----NGPSEALLSLDLLDKFWRLSLPNGATRDHHMA 250

Query: 265 NPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAM-AAAGKKVERVILKGVGHGF 318
           NP       L+ + L P++V +   ++L DR  E+   +    GK+V+ +  +   HGF
Sbjct: 251 NPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGF 304

BLAST of CmaCh01G004400 vs. ExPASy Swiss-Prot
Match: Q0ZPV7 (Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 2.0e-40
Identity = 107/306 (34.97%), Postives = 154/306 (50.33%), Query Frame = 0

Query: 14  MAKPHLQNGATSEEIEGLIR-----VYADGHVERPPIIPNVACS-TTASNKGVTAKDVLI 73
           M+  HL+   +S+    L++     +  D  + RP  IP+ A S    S+  V  KD+ +
Sbjct: 1   MSNDHLETTGSSDPNTNLLKYLPIVLNPDRTITRPIQIPSTAASPDPTSSSPVLTKDLAL 60

Query: 74  DIFTNTWARIYIPN----SQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVIL 133
           +   NT+ R+++P     +   LPL+VYFHGGGF + SAA + +H+F   +A  A  VI 
Sbjct: 61  NPLHNTFVRLFLPRHALYNSAKLPLVVYFHGGGFILFSAASTIFHDFCCEMAVHAGVVIA 120

Query: 134 SINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAGANI 193
           S++YRL+PE+RLPAAY+D   A+ W+K          +W     + S  F+ G+SAG NI
Sbjct: 121 SVDYRLAPEHRLPAAYDDAMEALQWIKDS------RDEWLTNFADFSNCFIMGESAGGNI 180

Query: 194 AYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSASDTY 253
           AY+   R       LL P  +KG++L +P FGG  RT SE   A  S      L   D  
Sbjct: 181 AYHAGLRAAAVADELL-PLKIKGLVLDEPGFGGSKRTGSELRLANDSRLPTFVL---DLI 240

Query: 254 WRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPL----MVCISELDILLDRNLEFCAAMA 306
           W LSLP  A+RDH + NP           K+  L    MV     D ++DR +E    + 
Sbjct: 241 WELSLPMGADRDHEYCNPTAESEPLYSFDKIRSLGWRVMVVGCHGDPMIDRQMELAERLE 296

BLAST of CmaCh01G004400 vs. ExPASy Swiss-Prot
Match: Q5NUF4 (2-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 3.7e-39
Identity = 111/326 (34.05%), Postives = 167/326 (51.23%), Query Frame = 0

Query: 27  EIEGLIRVYADGHVERPPIIPNVACSTTASNKGVTAKDVLIDIFTNTWARIYIP---NSQ 86
           E+  L+RVY DG VER      V  S      GV+ KD++I       AR+Y+P   N+ 
Sbjct: 16  ELPPLLRVYKDGTVERFLGSSFVPPSPEDPETGVSTKDIVISENPTISARVYLPKLNNTT 75

Query: 87  RNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPENRLPAAYEDGF 146
             LP+LVY+HGG FC+ SA    +  +L  +ASKA  +++SI YRL+PE+ LPAAYEDG+
Sbjct: 76  EKLPILVYYHGGAFCLESAFSFLHQRYLNIVASKANVLVVSIEYRLAPEHPLPAAYEDGW 135

Query: 147 NAVMWLKQQAITN--PIEHKWWL---GNCNPSKLFLAGDSAGANIAYNTATRLGHNESTL 206
            A+ W+   +  N  P     WL   G+ N  + ++ GD++GANIA+N A R+G     L
Sbjct: 136 YALKWVTSHSTNNNKPTNADPWLIKHGDFN--RFYIGGDTSGANIAHNAALRVG--AEAL 195

Query: 207 LKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSASDTYWRLSLPPT-ANRDHP 266
                + G++   P F G     SE +     S+           W    P      D+P
Sbjct: 196 PGGLRIAGVLSAFPLFWGSKPVLSEPVEGHEKSSPM-------QVWNFVYPDAPGGIDNP 255

Query: 267 WSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAAAGKK--VERVILKGVGH 326
             NPL  G   L  +  P ++V ++  D L DR + +  A+  +G K  VE    +G  H
Sbjct: 256 LINPLAPGAPNLATLGCPKMLVFVAGKDDLRDRGIWYYEAVKESGWKGDVELAQYEGEEH 315

Query: 327 GFQILNKDQISTQRTQELMGSIRAFI 342
            FQI + +   T+ +++L+G I +F+
Sbjct: 316 CFQIYHPE---TENSKDLIGRIASFL 327

BLAST of CmaCh01G004400 vs. ExPASy TrEMBL
Match: A0A6J1KCZ3 (probable carboxylesterase 17 OS=Cucurbita maxima OX=3661 GN=LOC111493192 PE=4 SV=1)

HSP 1 Score: 706.1 bits (1821), Expect = 7.4e-200
Identity = 344/344 (100.00%), Postives = 344/344 (100.00%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV 60
           MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV
Sbjct: 1   MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV 60

Query: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120
           TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC
Sbjct: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120

Query: 121 VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 180
           VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG
Sbjct: 121 VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 180

Query: 181 ANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240
           ANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS
Sbjct: 181 ANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240

Query: 241 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAA 300
           DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAA
Sbjct: 241 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAA 300

Query: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 345
           AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ
Sbjct: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 344

BLAST of CmaCh01G004400 vs. ExPASy TrEMBL
Match: A0A6J1G8X8 (probable carboxylesterase 17 OS=Cucurbita moschata OX=3662 GN=LOC111452001 PE=4 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 1.4e-195
Identity = 335/344 (97.38%), Postives = 341/344 (99.13%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV 60
           MAAMYIDPRLKLQMAKPHLQNG TSEEIEGLIRVYADGHVERPPIIPNVACSTTAS+KGV
Sbjct: 6   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 65

Query: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120
           TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC
Sbjct: 66  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 125

Query: 121 VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 180
           VILSINYRLSPENRLPAAY+DGFNA+MWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG
Sbjct: 126 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 185

Query: 181 ANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240
           ANI+YNTATR+GHNESTLLKP TLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSA+
Sbjct: 186 ANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAA 245

Query: 241 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAA 300
           DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMA 
Sbjct: 246 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAG 305

Query: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 345
           AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ
Sbjct: 306 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 349

BLAST of CmaCh01G004400 vs. ExPASy TrEMBL
Match: A0A6J1DL90 (probable carboxylesterase 17 OS=Momordica charantia OX=3673 GN=LOC111022166 PE=4 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 2.0e-168
Identity = 295/343 (86.01%), Postives = 311/343 (90.67%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV 60
           MAA+ IDPRLKLQM K HLQ  +T+EEIEGLIRVYADGHVERPPIIPNV CSTTASNKGV
Sbjct: 6   MAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGV 65

Query: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120
           +AKDVLID FTNTWARIYI NSQR LPLLVYFHGGGFCVGSAAWSCYHEFLL+LASKAKC
Sbjct: 66  SAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC 125

Query: 121 VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 180
            ILSINYRLSPENRLPAAYEDGFNA+MWLKQQAI+ P EHKWWLGNCNPS LFLAGDSAG
Sbjct: 126 AILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAG 185

Query: 181 ANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240
           ANIAYN ATR G NE   LKP TLKG+ILIQPFFGGESRTKSEKLAA P STSALTL+ +
Sbjct: 186 ANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQP-STSALTLATA 245

Query: 241 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAA 300
           DTYWRLSLPP A+RDHPWSNPLVNGG KL++ KLPP MVCISELDIL+DRNLEFCAAMAA
Sbjct: 246 DTYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAA 305

Query: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQ 344
           AGKKVERVI KGVGHGFQILNKDQIS  RTQE+M SI+ FIHQ
Sbjct: 306 AGKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFIHQ 347

BLAST of CmaCh01G004400 vs. ExPASy TrEMBL
Match: A0A2N9EQ59 (Abhydrolase_3 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS4850 PE=4 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 1.8e-129
Identity = 229/346 (66.18%), Postives = 271/346 (78.32%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV 60
           MAA+ +DPR KLQ+ K H Q+G   EEIEGLI+V  DGHVERPPI+P V C T A  +GV
Sbjct: 1   MAAISLDPRFKLQVGKNHHQHGGVVEEIEGLIKVCMDGHVERPPIVPIVPC-TMALERGV 60

Query: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120
           TA DV+ID  T+ WARIY+P+    LPLLVYFHGGGFCVGSAAWSCY+EFL NLASKA C
Sbjct: 61  TANDVVIDKCTSLWARIYVPSCSGKLPLLVYFHGGGFCVGSAAWSCYNEFLSNLASKANC 120

Query: 121 VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 180
           VI+S+NYRL+PENRLPAAYEDGFN +MW+KQQA+    EHKWWL +CN + +FL GDS+G
Sbjct: 121 VIISVNYRLAPENRLPAAYEDGFNTLMWVKQQALNGSNEHKWWLRHCNLTSMFLGGDSSG 180

Query: 181 ANIAYNTATRLGH---NESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTL 240
           ANIAYN ATRLG    +EST+++P  LKG ILIQPFFGGE+RT SEK    P++ S LTL
Sbjct: 181 ANIAYNVATRLGSSGTSESTIIRPLCLKGTILIQPFFGGEARTWSEKYTNQPAN-SGLTL 240

Query: 241 SASDTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAA 300
             SDTYWRLSLP   NRD+PW NPL NG AKL+E +LP +MVC+SE DIL DRNLEFC A
Sbjct: 241 LVSDTYWRLSLPIGTNRDNPWCNPLANGMAKLREFRLPSIMVCVSETDILKDRNLEFCTA 300

Query: 301 MAAAGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQ 344
           +  AGK+VE VI KGVGH FQIL+  Q+S  RT E+M  I+AFI++
Sbjct: 301 LTNAGKRVETVIYKGVGHAFQILHNSQLSQPRTHEMMAHIKAFINE 344

BLAST of CmaCh01G004400 vs. ExPASy TrEMBL
Match: F6HSF1 (Abhydrolase_3 domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_14s0006g03180 PE=4 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 2.0e-128
Identity = 229/344 (66.57%), Postives = 267/344 (77.62%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV 60
           MAA+ +DPRL LQM K   QNG   E++EGLIRVY DGHVERP I+PNV C T A   GV
Sbjct: 1   MAAISLDPRLSLQMGKNLYQNGVVVEKVEGLIRVYNDGHVERPAIVPNVPC-TVALELGV 60

Query: 61  TAKDVLIDIFTNTWARIYIPNSQR-NLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAK 120
           T KDV+I+ ++N WAR Y+P+     LPLLVYFHGGGFCVGSAAW+CYH FL +LASKA 
Sbjct: 61  TVKDVVIEKYSNLWARFYVPSCPAGKLPLLVYFHGGGFCVGSAAWNCYHGFLADLASKAG 120

Query: 121 CVILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSA 180
           C+I+S+NYRL+PENRLPAAYEDGFNAVMW+K QA+    E KWWL  CN S LFL GDSA
Sbjct: 121 CLIMSVNYRLAPENRLPAAYEDGFNAVMWVKNQALNGAGEQKWWLSRCNLSSLFLTGDSA 180

Query: 181 GANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSA 240
           GANIAYN ATRLG +++T LKP +LKG ILIQPFFGGE+RT SE  +  P + SALTLSA
Sbjct: 181 GANIAYNVATRLGSSDTTFLKPLSLKGTILIQPFFGGEARTGSENHSTQPPN-SALTLSA 240

Query: 241 SDTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMA 300
           SDTYWRLSLP  ANRDHP  NPL NG  KL+ ++LPP MVCIS+ DIL DRNL+FC AMA
Sbjct: 241 SDTYWRLSLPLGANRDHPCCNPLANGSTKLRTLQLPPTMVCISDTDILKDRNLQFCTAMA 300

Query: 301 AAGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQ 344
            AGK++E VI KGVGH FQ+L    +S  RT+E++  IRAFI Q
Sbjct: 301 NAGKRLETVIYKGVGHAFQVLQNSDLSQPRTKEMISHIRAFITQ 342

BLAST of CmaCh01G004400 vs. NCBI nr
Match: XP_022998605.1 (probable carboxylesterase 17 [Cucurbita maxima])

HSP 1 Score: 706.1 bits (1821), Expect = 1.5e-199
Identity = 344/344 (100.00%), Postives = 344/344 (100.00%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV 60
           MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV
Sbjct: 1   MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV 60

Query: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120
           TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC
Sbjct: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120

Query: 121 VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 180
           VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG
Sbjct: 121 VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 180

Query: 181 ANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240
           ANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS
Sbjct: 181 ANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240

Query: 241 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAA 300
           DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAA
Sbjct: 241 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAA 300

Query: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 345
           AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ
Sbjct: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 344

BLAST of CmaCh01G004400 vs. NCBI nr
Match: XP_022948283.1 (probable carboxylesterase 17 [Cucurbita moschata])

HSP 1 Score: 691.8 bits (1784), Expect = 3.0e-195
Identity = 335/344 (97.38%), Postives = 341/344 (99.13%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV 60
           MAAMYIDPRLKLQMAKPHLQNG TSEEIEGLIRVYADGHVERPPIIPNVACSTTAS+KGV
Sbjct: 6   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 65

Query: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120
           TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC
Sbjct: 66  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 125

Query: 121 VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 180
           VILSINYRLSPENRLPAAY+DGFNA+MWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG
Sbjct: 126 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 185

Query: 181 ANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240
           ANI+YNTATR+GHNESTLLKP TLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSA+
Sbjct: 186 ANISYNTATRIGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAA 245

Query: 241 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAA 300
           DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMA 
Sbjct: 246 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAG 305

Query: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 345
           AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ
Sbjct: 306 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 349

BLAST of CmaCh01G004400 vs. NCBI nr
Match: XP_023523365.1 (probable carboxylesterase 17 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 687.2 bits (1772), Expect = 7.3e-194
Identity = 333/344 (96.80%), Postives = 338/344 (98.26%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV 60
           MAAMY+DPRLKLQMAKPHLQNG TSEEIEGLIRVYADGHVERPPIIPNVACSTTAS+KGV
Sbjct: 6   MAAMYVDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 65

Query: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120
           TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC
Sbjct: 66  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 125

Query: 121 VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 180
           VILSINYRLSPENRLPAAY+DGFNA+MWLKQQAITNPIEHKWW GNCNPSKLFLAGDSAG
Sbjct: 126 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNPIEHKWWSGNCNPSKLFLAGDSAG 185

Query: 181 ANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240
           ANI YNTATRLGHNESTLLKP TLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS
Sbjct: 186 ANIVYNTATRLGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 245

Query: 241 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAA 300
           DTYWRLSLPPTANRDHPWSNPLVNGGAKLKE KLPPLMVCISELDILLDRNLEFCAAMA 
Sbjct: 246 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKETKLPPLMVCISELDILLDRNLEFCAAMAG 305

Query: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 345
           AGKKV+RVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ
Sbjct: 306 AGKKVDRVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 349

BLAST of CmaCh01G004400 vs. NCBI nr
Match: KAG6607091.1 (putative carboxylesterase 17, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036780.1 putative carboxylesterase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 685.6 bits (1768), Expect = 2.1e-193
Identity = 335/344 (97.38%), Postives = 338/344 (98.26%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV 60
           MAAMYIDPRLKLQMAKPHLQNG TSEEIEGLIRVYADGHVERPPIIPNVACSTTAS+KGV
Sbjct: 1   MAAMYIDPRLKLQMAKPHLQNGGTSEEIEGLIRVYADGHVERPPIIPNVACSTTASDKGV 60

Query: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120
           TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC
Sbjct: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120

Query: 121 VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 180
           VILSINYRLSPENRLPAAY+DGFNA+MWLKQQAITN IEHKWWLGNCNPSKLFLAGDSAG
Sbjct: 121 VILSINYRLSPENRLPAAYDDGFNALMWLKQQAITNAIEHKWWLGNCNPSKLFLAGDSAG 180

Query: 181 ANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240
           ANIAYNTATRLGHNESTLLKP TLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS
Sbjct: 181 ANIAYNTATRLGHNESTLLKPLTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240

Query: 241 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAA 300
           DTYWRLSLPPTANRDHPWSNPLVNG AKLKEMKLPPLMV ISELDILLDRNLEFCAAMA 
Sbjct: 241 DTYWRLSLPPTANRDHPWSNPLVNGSAKLKEMKLPPLMVSISELDILLDRNLEFCAAMAG 300

Query: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 345
           AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ
Sbjct: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQQ 344

BLAST of CmaCh01G004400 vs. NCBI nr
Match: XP_022155020.1 (probable carboxylesterase 17 [Momordica charantia])

HSP 1 Score: 601.7 bits (1550), Expect = 4.1e-168
Identity = 295/343 (86.01%), Postives = 311/343 (90.67%), Query Frame = 0

Query: 1   MAAMYIDPRLKLQMAKPHLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGV 60
           MAA+ IDPRLKLQM K HLQ  +T+EEIEGLIRVYADGHVERPPIIPNV CSTTASNKGV
Sbjct: 6   MAAISIDPRLKLQMPKSHLQCASTAEEIEGLIRVYADGHVERPPIIPNVPCSTTASNKGV 65

Query: 61  TAKDVLIDIFTNTWARIYIPNSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKC 120
           +AKDVLID FTNTWARIYI NSQR LPLLVYFHGGGFCVGSAAWSCYHEFLL+LASKAKC
Sbjct: 66  SAKDVLIDNFTNTWARIYIANSQRKLPLLVYFHGGGFCVGSAAWSCYHEFLLDLASKAKC 125

Query: 121 VILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAG 180
            ILSINYRLSPENRLPAAYEDGFNA+MWLKQQAI+ P EHKWWLGNCNPS LFLAGDSAG
Sbjct: 126 AILSINYRLSPENRLPAAYEDGFNALMWLKQQAISEPNEHKWWLGNCNPSNLFLAGDSAG 185

Query: 181 ANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSAS 240
           ANIAYN ATR G NE   LKP TLKG+ILIQPFFGGESRTKSEKLAA P STSALTL+ +
Sbjct: 186 ANIAYNIATRPGLNEPATLKPLTLKGVILIQPFFGGESRTKSEKLAAQP-STSALTLATA 245

Query: 241 DTYWRLSLPPTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAA 300
           DTYWRLSLPP A+RDHPWSNPLVNGG KL++ KLPP MVCISELDIL+DRNLEFCAAMAA
Sbjct: 246 DTYWRLSLPPGASRDHPWSNPLVNGGTKLRDTKLPPSMVCISELDILVDRNLEFCAAMAA 305

Query: 301 AGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFIHQ 344
           AGKKVERVI KGVGHGFQILNKDQIS  RTQE+M SI+ FIHQ
Sbjct: 306 AGKKVERVIFKGVGHGFQILNKDQISAIRTQEMMVSIQNFIHQ 347

BLAST of CmaCh01G004400 vs. TAIR 10
Match: AT5G16080.1 (carboxyesterase 17 )

HSP 1 Score: 345.1 bits (884), Expect = 6.4e-95
Identity = 183/333 (54.95%), Postives = 228/333 (68.47%), Query Frame = 0

Query: 18  HLQNGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGVTAKDVLIDIFTNTWARI 77
           H ++G   EEIEGLI+V+ DG VERPPI+P V+ +   S+K  TA D  I +  +TW R+
Sbjct: 22  HHRHGPVVEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSK-ATAFD--IKLSNDTWTRV 81

Query: 78  YIPNSQR-----NLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPE 137
           YIP++        LPLLVYFHGGGFCVGSAAWSCYH+FL +LA KA+CVI+S+NYRL+PE
Sbjct: 82  YIPDAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPE 141

Query: 138 NRLPAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAGANIAYNTATRL- 197
           +RLPAAY+DG N V WL +Q I+    +  WL  CN S +FLAGDSAGANIAY  A R+ 
Sbjct: 142 HRLPAAYDDGVNVVSWLVKQQISTGGGYPSWLSKCNLSNVFLAGDSAGANIAYQVAVRIM 201

Query: 198 --GHNESTLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSASDTYWRLSLP 257
             G   +TL     LKGIILI PFFGGESRT SEK     + +SALTLSASD YWRL+LP
Sbjct: 202 ASGKYANTL----HLKGIILIHPFFGGESRTSSEK-QQHHTKSSALTLSASDAYWRLALP 261

Query: 258 PTANRDHPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAAAGKKVERVI 317
             A+RDHPW NPL++        KLP  MV ++E DIL +RNLE C  M + GK+VE ++
Sbjct: 262 RGASRDHPWCNPLMSSAG----AKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIV 321

Query: 318 LKGVGHGFQILNKDQISTQRTQELMGSIRAFIH 343
             GVGH F IL+   +S  R  ++M  +  FIH
Sbjct: 322 HGGVGHAFHILDNSSVSRDRIHDMMCRLHNFIH 342

BLAST of CmaCh01G004400 vs. TAIR 10
Match: AT1G68620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 307.8 bits (787), Expect = 1.1e-83
Identity = 156/327 (47.71%), Postives = 213/327 (65.14%), Query Frame = 0

Query: 21  NGATSEEIEGLIRVYADGHVERPPIIPNVACSTTASNKGVTAKDVLIDIFTNTWARIYIP 80
           +G   +E+EGLI+VY DGHVER  ++P V  S      GVT  DV+ID  TN WAR+Y+P
Sbjct: 20  HGPVVDEVEGLIKVYKDGHVERSQLLPCVDPSLPL-ELGVTCSDVVIDKLTNVWARLYVP 79

Query: 81  -----NSQRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPENRL 140
                +S   LPL+VYFHGGGFCVGSA+W CYHEFL  L+++++C+++S+NYRL+PEN L
Sbjct: 80  MTTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPL 139

Query: 141 PAAYEDGFNAVMWLKQQAITNPIEHKWWLGNCNPSKLFLAGDSAGANIAYNTATRLGHNE 200
           PAAYEDG NA++WL +    N      W   C+  ++FLAGDSAG NIA   A RL   E
Sbjct: 140 PAAYEDGVNAILWLNKARNDN-----LWAKQCDFGRIFLAGDSAGGNIAQQVAARLASPE 199

Query: 201 STLLKPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSASDTYWRLSLPPTANRD 260
              LK   ++G ILIQPF+ GE RT+SE+       T+ LTL++SD +WR+SLP  ANR+
Sbjct: 200 DLALK---IEGTILIQPFYSGEERTESERRVGN-DKTAVLTLASSDAWWRMSLPRGANRE 259

Query: 261 HPWSNPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAMAAAGKKVERVILKGVGH 320
           HP+  P+      +K   +   +VC++E+D+L+D N+E C         ++RV+ KGVGH
Sbjct: 260 HPYCKPV---KMIIKSSTVTRTLVCVAEMDLLMDSNMEMCDGNEDV---IKRVLHKGVGH 319

Query: 321 GFQILNKDQISTQRTQELMGSIRAFIH 343
            F IL K Q++   T E++  I AFIH
Sbjct: 320 AFHILGKSQLAHTTTLEMLCQIDAFIH 330

BLAST of CmaCh01G004400 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 192.2 bits (487), Expect = 6.9e-49
Identity = 111/299 (37.12%), Postives = 170/299 (56.86%), Query Frame = 0

Query: 25  SEEIEGLIRVYADGHVERPPIIPNVACSTT-ASNKGVTAKDVLIDIFTNTWARIYIPNSQ 84
           +E+  GL+++ ++G V R   I  +       +N+ V  KD +     N   R+Y P S 
Sbjct: 11  AEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPISA 70

Query: 85  RN---LPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPENRLPAAYE 144
            N   LP++V+FHGGGFC GS +W  +H F L LAS    +++S +YRL+PE+RLPAA+E
Sbjct: 71  SNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFE 130

Query: 145 DGFNAVMWLKQQAITNPIEHKWWLG-NCNPSKLFLAGDSAGANIAYNTATRLGHNESTLL 204
           D    + WL  QA+++ + H +  G + +  ++F+ GDS+G NIA+  A R G + S  L
Sbjct: 131 DAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-SGSIEL 190

Query: 205 KPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSASDTYWRLSLPPTANRDHPWS 264
            P  ++G +L+ PFFGGE RT SE       S + L+L   D +WRLSLP  A RDH  +
Sbjct: 191 TPVRVRGYVLMGPFFGGEERTNSE----NGPSEALLSLDLLDKFWRLSLPNGATRDHHMA 250

Query: 265 NPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAM-AAAGKKVERVILKGVGHGF 318
           NP       L+ + L P++V +   ++L DR  E+   +    GK+V+ +  +   HGF
Sbjct: 251 NPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGF 304

BLAST of CmaCh01G004400 vs. TAIR 10
Match: AT5G06570.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 192.2 bits (487), Expect = 6.9e-49
Identity = 111/299 (37.12%), Postives = 170/299 (56.86%), Query Frame = 0

Query: 25  SEEIEGLIRVYADGHVERPPIIPNVACSTT-ASNKGVTAKDVLIDIFTNTWARIYIPNSQ 84
           +E+  GL+++ ++G V R   I  +       +N+ V  KD +     N   R+Y P S 
Sbjct: 11  AEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPISA 70

Query: 85  RN---LPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKAKCVILSINYRLSPENRLPAAYE 144
            N   LP++V+FHGGGFC GS +W  +H F L LAS    +++S +YRL+PE+RLPAA+E
Sbjct: 71  SNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFE 130

Query: 145 DGFNAVMWLKQQAITNPIEHKWWLG-NCNPSKLFLAGDSAGANIAYNTATRLGHNESTLL 204
           D    + WL  QA+++ + H +  G + +  ++F+ GDS+G NIA+  A R G + S  L
Sbjct: 131 DAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-SGSIEL 190

Query: 205 KPPTLKGIILIQPFFGGESRTKSEKLAATPSSTSALTLSASDTYWRLSLPPTANRDHPWS 264
            P  ++G +L+ PFFGGE RT SE       S + L+L   D +WRLSLP  A RDH  +
Sbjct: 191 TPVRVRGYVLMGPFFGGEERTNSE----NGPSEALLSLDLLDKFWRLSLPNGATRDHHMA 250

Query: 265 NPLVNGGAKLKEMKLPPLMVCISELDILLDRNLEFCAAM-AAAGKKVERVILKGVGHGF 318
           NP       L+ + L P++V +   ++L DR  E+   +    GK+V+ +  +   HGF
Sbjct: 251 NPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGF 304

BLAST of CmaCh01G004400 vs. TAIR 10
Match: AT2G45600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 161.8 bits (408), Expect = 1.0e-39
Identity = 101/295 (34.24%), Postives = 155/295 (52.54%), Query Frame = 0

Query: 62  AKDVLIDIFTNTWARIYIPNS---QRNLPLLVYFHGGGFCVGSAAWSCYHEFLLNLASKA 121
           +KD+ ++   NT+ RI+ P +   +  LP+LVYFHGGGF + SAA + +HE    +A + 
Sbjct: 39  SKDIPLNQTNNTFIRIFKPRNIPPESKLPILVYFHGGGFILYSAASAPFHESCTKMADRL 98

Query: 122 KCVILSINYRLSPENRLPAAYEDGFNAVMWLKQQAITNPIE----HKWWLGNCNPSKLFL 181
           + +ILS+ YRL+PE+RLPAAYED   A++WL+ QA   PI       W     + SK ++
Sbjct: 99  QTIILSVEYRLAPEHRLPAAYEDAVEAILWLRDQA-RGPINGGDCDTWLKDGVDFSKCYV 158

Query: 182 AGDSAGANIAYNTATRLGHNESTLLKPPTLKGIILIQPFFGG------ESRTKSEKLAAT 241
            G S+G NI YN A R+     T L P  ++G+I+ Q FFGG      ESR K +K+   
Sbjct: 159 MGSSSGGNIVYNVALRV---VDTDLSPVKIQGLIMNQAFFGGVEPSDSESRLKDDKICPL 218

Query: 242 PSSTSALTLSASDTYWRLSLPPTANRDHPWSNPLVNGGAKLKEM--KLPPLMVCISELDI 301
           P         A+   W L LP   +RDH +SNP+ + G + K+   + P  ++     D 
Sbjct: 219 P---------ATHLLWSLCLPDGVDRDHVYSNPIKSSGPQEKDKMGRFPSTLINGYGGDP 278

Query: 302 LLDRNLEFCAAMAAAGKKVERVILKGVGHGFQILNKDQISTQRTQELMGSIRAFI 342
           L+DR       +   G  VE    K   H  ++ + +     + + L  ++ AF+
Sbjct: 279 LVDRQRHVAEMLKGRGVHVETRFDKDGFHACELFDGN-----KAKALYETVEAFM 315

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LFR79.0e-9454.95Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1[more]
Q9SX251.6e-8247.71Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1[more]
Q9FG139.7e-4837.12Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1[more]
Q0ZPV72.0e-4034.97Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1[more]
Q5NUF43.7e-3934.052-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1KCZ37.4e-200100.00probable carboxylesterase 17 OS=Cucurbita maxima OX=3661 GN=LOC111493192 PE=4 SV... [more]
A0A6J1G8X81.4e-19597.38probable carboxylesterase 17 OS=Cucurbita moschata OX=3662 GN=LOC111452001 PE=4 ... [more]
A0A6J1DL902.0e-16886.01probable carboxylesterase 17 OS=Momordica charantia OX=3673 GN=LOC111022166 PE=4... [more]
A0A2N9EQ591.8e-12966.18Abhydrolase_3 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS... [more]
F6HSF12.0e-12866.57Abhydrolase_3 domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_14s000... [more]
Match NameE-valueIdentityDescription
XP_022998605.11.5e-199100.00probable carboxylesterase 17 [Cucurbita maxima][more]
XP_022948283.13.0e-19597.38probable carboxylesterase 17 [Cucurbita moschata][more]
XP_023523365.17.3e-19496.80probable carboxylesterase 17 [Cucurbita pepo subsp. pepo][more]
KAG6607091.12.1e-19397.38putative carboxylesterase 17, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_022155020.14.1e-16886.01probable carboxylesterase 17 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G16080.16.4e-9554.95carboxyesterase 17 [more]
AT1G68620.11.1e-8347.71alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.16.9e-4937.12alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.26.9e-4937.12alpha/beta-Hydrolases superfamily protein [more]
AT2G45600.11.0e-3934.24alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 32..343
e-value: 1.0E-78
score: 267.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 30..343
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 89..318
e-value: 9.1E-58
score: 195.6
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 15..342
NoneNo IPR availablePANTHERPTHR23024:SF409CARBOXYLESTERASE 6-RELATEDcoord: 15..342
IPR033140Lipase, GDXG, putative serine active sitePROSITEPS01174LIPASE_GDXG_SERcoord: 172..184

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G004400.1CmaCh01G004400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity