CmaCh01G003520 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh01G003520
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	2-isopropylmalate synthase
Location	Cma_Chr01: 1745352 .. 1747016 (+)
RNA-Seq Expression	CmaCh01G003520
Synteny	CmaCh01G003520

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGGAACCAGTAACACTGCTCGATTATTCAATGAATTCTGTCACCGAAGGGATCGATGCAATTGCCACTACTCGTGTTGTAATAAGAGGGGACAACAGTTACTTATCTACTAATGCATCGGCTGGAGAAGAAGTTCAACGAACGTTTAGGTATACATTTTCTTCCCTATCATTTAAAGTAATTAAACTTAGCTATTATTTTCTAAATTTCTTCAATTTTGCCCATCTCGTTGTCTGTTGGTACACTTAACCAACATGGCATGATGTTTAAGGAGTTAAGACAAAACCTTAGAAGAGCTTAAGCGGGAAAATTTTCTAAGTTTGTCTCACATTCCTATTTAAGTTGGCAAAAATTCCAAATTTTCTGAGGTGATCGTTCCTTCTCTGATTTTTGCCAATGACTATATAGCATTCCTGGAAGAACATAAAAGGGTATAATTAATAAAAATTTAAAACTTAAGGGACTAAATTGAAAGAACACTCCAACATTAGTGACTAAACTGTATATTTTAACAATTTTTAAATACAATCATATTCAAGTACTTTTGCCTCTTTGTTATTGGATCTAGGCTTCCTTATGTTTAACTTGAGAAGACTAGGATGGCCATTAGTATCGTGAAAAATAAAGGGATTTAGAAGGAATGAATTCAAACTAGGGTAGTAACCTATATGGAGAATTTAAACGTTTTGTCTTGCAACTAATTGTACTTGTAAGCTTAGTTGGTTGTCTCCCTGTTTCCAGCCTCTCTCCCCCTAACCTAACTAGTTGGTTGTCTCCCTGTTTCCAGCCTCTCTCCCCCTAACCTAACAGGCGAAGTTCACAACCTTGAGCTCTAGATTTCGATAGGAACAACCACATCACTACTAGTCTGCAAAAAAAAAAAAAAAAAGGACGGACTTGGCTTCATTTCTCACTCTATCAAAAATTACGTAACGTATAAGGACATATAAGGATAAGAAGCACATACATATAGAGAGGTGAAAATGACGGAGCTGACCAACTATGATATAGATATTTTACTTAGCTCCATTTTCTCGGTTCAGGTATGGGCAAACCTAATTAGATGCCTACCAATTATAAAAAATAGAATAGAGGCAATGTGGATCCACCTATATATAGGGAAGGGTTCAAACTACGGTGGCTATCTACTTTGGATTTAACTTCCTATGAGTTCTTCTGTATAATTAGACAAAATGTGTGCAAGTTGACTCGTTTACTCGCTGGTTAAAATTAAGAGGACCAACCGATATCAACCCATATGAAATTCAACGTGAATTCTGTTACTGTTTTATATTCTTCTCTGGCTACAGTACATCGATGACTCGATATTTAGTGATATGTTTTTATCGTTCCATTGCAGTGGTATCGGAGCGGGAATGGACATCATCGTGGCAAGTCTCAAGGCCTACATTGGTGCATTAAACAAGATGCTTGGTTTCTACGGAGTTGATGTTAAGGTGACCGAAAAAATAACAGTCTGTATAAAAATTGTTTATTTGAGAAGAGGATGATGGTGGCAATCTTACATTCTCAACATGCTTGAAAGCTTTTAGCATTTTGGGTTAAGTTTGCTTTAATTATCAGTATTTGGTTGGTTGTGTTAGACCGCGTTTTAAACTTCGGTGAACTTTGTGTTAGTTAAAGTTGAACAAAAAAGGGAAA

mRNA sequence

ATGCAGGAACCAGTAACACTGCTCGATTATTCAATGAATTCTGTCACCGAAGGGATCGATGCAATTGCCACTACTCGTGTTGTAATAAGAGGGGACAACAGTTACTTATCTACTAATGCATCGGCTGGAGAAGAAGTTCAACGAACGTTTAGTGGTATCGGAGCGGGAATGGACATCATCGTGGCAAGTCTCAAGGCCTACATTGGTGCATTAAACAAGATGCTTGGTTTCTACGGAGTTGATGTTAAGGTGACCGAAAAAATAACAGTCTGTATAAAAATTGTTTATTTGAGAAGAGGATGATGGTGGCAATCTTACATTCTCAACATGCTTGAAAGCTTTTAGCATTTTGGGTTAAGTTTGCTTTAATTATCAGTATTTGGTTGGTTGTGTTAGACCGCGTTTTAAACTTCGGTGAACTTTGTGTTAGTTAAAGTTGAACAAAAAAGGGAAA

Coding sequence (CDS)

ATGCAGGAACCAGTAACACTGCTCGATTATTCAATGAATTCTGTCACCGAAGGGATCGATGCAATTGCCACTACTCGTGTTGTAATAAGAGGGGACAACAGTTACTTATCTACTAATGCATCGGCTGGAGAAGAAGTTCAACGAACGTTTAGTGGTATCGGAGCGGGAATGGACATCATCGTGGCAAGTCTCAAGGCCTACATTGGTGCATTAAACAAGATGCTTGGTTTCTACGGAGTTGATGTTAAGGTGACCGAAAAAATAACAGTCTGTATAAAAATTGTTTATTTGAGAAGAGGATGA

Protein sequence

MQEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASAGEEVQRTFSGIGAGMDIIVASLKAYIGALNKMLGFYGVDVKVTEKITVCIKIVYLRRG

Homology

[Close]

BLAST of CmaCh01G003520 vs. ExPASy Swiss-Prot
Match: Q9C550 (2-isopropylmalate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPMS2 PE=1 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 5.5e-28
Identity = 60/77 (77.92%), Postives = 71/77 (92.21%), Query Frame = 0


Query: 1   MQEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASAGEEVQRTFSGIGAGMDII 60
           ++EP TLL+YSMN+VTEGIDAIATTRV+IRGDN+Y STNA  GE V+RTFSG GAGMDI+
Sbjct: 536 VKEPATLLEYSMNAVTEGIDAIATTRVLIRGDNNYSSTNAVTGESVERTFSGTGAGMDIV 595

Query: 61  VASLKAYIGALNKMLGF 78
           V+S+KAY+GALNKMLGF
Sbjct: 596 VSSVKAYVGALNKMLGF 612

[Close]

BLAST of CmaCh01G003520 vs. ExPASy Swiss-Prot
Match: Q9LPR4 (2-isopropylmalate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPMS1 PE=1 SV=2)

HSP 1 Score: 121.7 bits (304), Expect = 4.7e-27
Identity = 59/77 (76.62%), Postives = 69/77 (89.61%), Query Frame = 0


Query: 1   MQEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASAGEEVQRTFSGIGAGMDII 60
           ++EP TLL+YSMN+VTEGIDAIATTRV+IRG N Y STNA  GEEVQRTFSG GAGMDI+
Sbjct: 538 VKEPATLLEYSMNAVTEGIDAIATTRVLIRGSNKYSSTNAITGEEVQRTFSGTGAGMDIV 597

Query: 61  VASLKAYIGALNKMLGF 78
           V+S+KAY+GALNKM+ F
Sbjct: 598 VSSVKAYVGALNKMMDF 614

[Close]

BLAST of CmaCh01G003520 vs. ExPASy Swiss-Prot
Match: O04974 (2-isopropylmalate synthase B OS=Solanum pennellii OX=28526 GN=IPMSB PE=2 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 1.6e-22
Identity = 50/74 (67.57%), Postives = 65/74 (87.84%), Query Frame = 0


Query: 4   PVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASAGEEVQRTFSGIGAGMDIIVAS 63
           PVTLL+YSMN+VT+GIDAIA+TRV+IRG+N + ST+A  GE V RTFSG GA MDI+++S
Sbjct: 523 PVTLLEYSMNAVTQGIDAIASTRVLIRGENGHTSTHALTGETVHRTFSGTGADMDIVISS 582

Query: 64  LKAYIGALNKMLGF 78
           ++AY+GALNKM+ F
Sbjct: 583 VRAYVGALNKMMSF 596

[Close]

BLAST of CmaCh01G003520 vs. ExPASy Swiss-Prot
Match: O04973 (2-isopropylmalate synthase A OS=Solanum pennellii OX=28526 GN=IPMSA PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 2.3e-18
Identity = 44/74 (59.46%), Postives = 59/74 (79.73%), Query Frame = 0


Query: 4   PVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASAGEEVQRTFSGIGAGMDIIVAS 63
           P+TLL+YSMN+VTEGIDAIA+TRV I   + +   N S G+ + RTFSG GA MD++++S
Sbjct: 500 PITLLEYSMNAVTEGIDAIASTRVSICSIDRHTIMNGSTGQTIHRTFSGTGADMDVVISS 559

Query: 64  LKAYIGALNKMLGF 78
           ++AYIGALNKML +
Sbjct: 560 VRAYIGALNKMLSY 573

[Close]

BLAST of CmaCh01G003520 vs. ExPASy Swiss-Prot
Match: Q39891 (Probable 2-isopropylmalate synthase OS=Glycine max OX=3847 GN=GMN56 PE=2 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 1.7e-13
Identity = 40/75 (53.33%), Postives = 54/75 (72.00%), Query Frame = 0


Query: 1   MQEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASAGEEVQRTFSGIGAGMDII 60
           ++EP  LLDYS+NSVTEGI    T RVVI  +N++ ST A   +    TFSGI A MD++
Sbjct: 482 VKEPTKLLDYSLNSVTEGIGVNVTARVVICRENNHTSTYAFTEDANYPTFSGIAAEMDVV 541

Query: 61  VASLKAYIGALNKML 76
           V+++KAY+ ALNK+L
Sbjct: 542 VSTVKAYLVALNKLL 556

[Close]

BLAST of CmaCh01G003520 vs. ExPASy TrEMBL
Match: A0A6J1K8I4 (2-isopropylmalate synthase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111493218 PE=4 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 4.7e-46
Identity = 100/100 (100.00%), Postives = 100/100 (100.00%), Query Frame = 0


Query: 1   MQEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASAGEEVQRTFSGIGAGMDII 60
           MQEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASAGEEVQRTFSGIGAGMDII
Sbjct: 1   MQEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASAGEEVQRTFSGIGAGMDII 60

Query: 61  VASLKAYIGALNKMLGFYGVDVKVTEKITVCIKIVYLRRG 101
           VASLKAYIGALNKMLGFYGVDVKVTEKITVCIKIVYLRRG
Sbjct: 61  VASLKAYIGALNKMLGFYGVDVKVTEKITVCIKIVYLRRG 100

[Close]

BLAST of CmaCh01G003520 vs. ExPASy TrEMBL
Match: A0A6J1G9D1 (2-isopropylmalate synthase OS=Cucurbita moschata OX=3662 GN=LOC111452159 PE=3 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 4.7e-38
Identity = 85/89 (95.51%), Postives = 88/89 (98.88%), Query Frame = 0


Query: 1   MQEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASAGEEVQRTFSGIGAGMDII 60
           ++EPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNAS GEEVQRTFSGIGAGMDI+
Sbjct: 538 IKEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDIV 597

Query: 61  VASLKAYIGALNKMLGFYGVDVKVTEKIT 90
           VASLKAYIGALNKMLGFYGVDVKVTEKIT
Sbjct: 598 VASLKAYIGALNKMLGFYGVDVKVTEKIT 626

[Close]

BLAST of CmaCh01G003520 vs. ExPASy TrEMBL
Match: A0A6J1CP08 (2-isopropylmalate synthase 2, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111013233 PE=4 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 6.6e-32
Identity = 73/90 (81.11%), Postives = 83/90 (92.22%), Query Frame = 0


Query: 1   MQEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASAGEEVQRTFSGIGAGMDII 60
           ++EPVTLL+YSMN+VTEGIDAIATTRVVIRG+NSY STNA  GE VQRTFSGIGAGMDI+
Sbjct: 140 VKEPVTLLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAMTGEAVQRTFSGIGAGMDIV 199

Query: 61  VASLKAYIGALNKMLGFYGVDVKVTEKITV 91
            +S+KAYIGALNKMLGF GVD+KVTEK T+
Sbjct: 200 XSSVKAYIGALNKMLGFQGVDIKVTEKRTL 229

[Close]

BLAST of CmaCh01G003520 vs. ExPASy TrEMBL
Match: A0A6J1CKJ3 (2-isopropylmalate synthase OS=Momordica charantia OX=3673 GN=LOC111012138 PE=3 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 8.6e-32
Identity = 73/90 (81.11%), Postives = 83/90 (92.22%), Query Frame = 0


Query: 1   MQEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASAGEEVQRTFSGIGAGMDII 60
           ++EPV LL+YSMN+VTEGIDAIATTRVVIRG+NSY STNA  GE VQRTFSGIGAGMDI+
Sbjct: 531 VKEPVALLEYSMNAVTEGIDAIATTRVVIRGENSYTSTNAITGEAVQRTFSGIGAGMDIV 590

Query: 61  VASLKAYIGALNKMLGFYGVDVKVTEKITV 91
           V+S+KAYIGALNKMLGF GVD+KVTEK T+
Sbjct: 591 VSSVKAYIGALNKMLGFQGVDIKVTEKRTL 620

[Close]

BLAST of CmaCh01G003520 vs. ExPASy TrEMBL
Match: A0A0A0L7C8 (2-isopropylmalate synthase OS=Cucumis sativus OX=3659 GN=Csa_3G363180 PE=3 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 9.5e-31
Identity = 70/90 (77.78%), Postives = 82/90 (91.11%), Query Frame = 0


Query: 1   MQEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASAGEEVQRTFSGIGAGMDII 60
           ++EP TLL+YSMN+VTEGIDAIATTRV+IRGD SY STNA  GE VQRTFSGIGAGMDI+
Sbjct: 542 VKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMDIV 601

Query: 61  VASLKAYIGALNKMLGFYGVDVKVTEKITV 91
           V+S+KAYIGALNKMLGF G+D+KVTE+ T+
Sbjct: 602 VSSVKAYIGALNKMLGFQGIDIKVTEEKTL 631

[Close]

BLAST of CmaCh01G003520 vs. NCBI nr
Match: XP_022998632.1 (2-isopropylmalate synthase 2, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 193.4 bits (490), Expect = 9.7e-46
Identity = 100/100 (100.00%), Postives = 100/100 (100.00%), Query Frame = 0


Query: 1   MQEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASAGEEVQRTFSGIGAGMDII 60
           MQEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASAGEEVQRTFSGIGAGMDII
Sbjct: 1   MQEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASAGEEVQRTFSGIGAGMDII 60

Query: 61  VASLKAYIGALNKMLGFYGVDVKVTEKITVCIKIVYLRRG 101
           VASLKAYIGALNKMLGFYGVDVKVTEKITVCIKIVYLRRG
Sbjct: 61  VASLKAYIGALNKMLGFYGVDVKVTEKITVCIKIVYLRRG 100

[Close]

BLAST of CmaCh01G003520 vs. NCBI nr
Match: XP_022948501.1 (2-isopropylmalate synthase 2, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 166.8 bits (421), Expect = 9.7e-38
Identity = 85/89 (95.51%), Postives = 88/89 (98.88%), Query Frame = 0


Query: 1   MQEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASAGEEVQRTFSGIGAGMDII 60
           ++EPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNAS GEEVQRTFSGIGAGMDI+
Sbjct: 538 IKEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDIV 597

Query: 61  VASLKAYIGALNKMLGFYGVDVKVTEKIT 90
           VASLKAYIGALNKMLGFYGVDVKVTEKIT
Sbjct: 598 VASLKAYIGALNKMLGFYGVDVKVTEKIT 626

[Close]

BLAST of CmaCh01G003520 vs. NCBI nr
Match: KAG6607000.1 (2-isopropylmalate synthase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 166.8 bits (421), Expect = 9.7e-38
Identity = 85/89 (95.51%), Postives = 88/89 (98.88%), Query Frame = 0


Query: 1   MQEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASAGEEVQRTFSGIGAGMDII 60
           ++EPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNAS GEEVQRTFSGIGAGMDI+
Sbjct: 538 IKEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDIV 597

Query: 61  VASLKAYIGALNKMLGFYGVDVKVTEKIT 90
           VASLKAYIGALNKMLGFYGVDVKVTEKIT
Sbjct: 598 VASLKAYIGALNKMLGFYGVDVKVTEKIT 626

[Close]

BLAST of CmaCh01G003520 vs. NCBI nr
Match: KAG7036701.1 (2-isopropylmalate synthase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 166.8 bits (421), Expect = 9.7e-38
Identity = 85/89 (95.51%), Postives = 88/89 (98.88%), Query Frame = 0


Query: 1   MQEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASAGEEVQRTFSGIGAGMDII 60
           ++EPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNAS GEEVQRTFSGIGAGMDI+
Sbjct: 538 IKEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDIV 597

Query: 61  VASLKAYIGALNKMLGFYGVDVKVTEKIT 90
           VASLKAYIGALNKMLGFYGVDVKVTEKIT
Sbjct: 598 VASLKAYIGALNKMLGFYGVDVKVTEKIT 626

[Close]

BLAST of CmaCh01G003520 vs. NCBI nr
Match: XP_023522725.1 (2-isopropylmalate synthase 2, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 165.2 bits (417), Expect = 2.8e-37
Identity = 84/89 (94.38%), Postives = 88/89 (98.88%), Query Frame = 0


Query: 1   MQEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASAGEEVQRTFSGIGAGMDII 60
           ++EPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNAS GEEVQRTFSGIGAGMDI+
Sbjct: 538 IKEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDIV 597

Query: 61  VASLKAYIGALNKMLGFYGVDVKVTEKIT 90
           VASLKAYIGALNKMLGF+GVDVKVTEKIT
Sbjct: 598 VASLKAYIGALNKMLGFFGVDVKVTEKIT 626

[Close]

BLAST of CmaCh01G003520 vs. TAIR 10
Match: AT1G74040.1 (2-isopropylmalate synthase 1 )

HSP 1 Score: 124.8 bits (312), Expect = 3.9e-29
Identity = 60/77 (77.92%), Postives = 71/77 (92.21%), Query Frame = 0


Query: 1   MQEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASAGEEVQRTFSGIGAGMDII 60
           ++EP TLL+YSMN+VTEGIDAIATTRV+IRGDN+Y STNA  GE V+RTFSG GAGMDI+
Sbjct: 536 VKEPATLLEYSMNAVTEGIDAIATTRVLIRGDNNYSSTNAVTGESVERTFSGTGAGMDIV 595

Query: 61  VASLKAYIGALNKMLGF 78
           V+S+KAY+GALNKMLGF
Sbjct: 596 VSSVKAYVGALNKMLGF 612

[Close]

BLAST of CmaCh01G003520 vs. TAIR 10
Match: AT1G18500.1 (methylthioalkylmalate synthase-like 4 )

HSP 1 Score: 121.7 bits (304), Expect = 3.3e-28
Identity = 59/77 (76.62%), Postives = 69/77 (89.61%), Query Frame = 0


Query: 1   MQEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASAGEEVQRTFSGIGAGMDII 60
           ++EP TLL+YSMN+VTEGIDAIATTRV+IRG N Y STNA  GEEVQRTFSG GAGMDI+
Sbjct: 538 VKEPATLLEYSMNAVTEGIDAIATTRVLIRGSNKYSSTNAITGEEVQRTFSGTGAGMDIV 597

Query: 61  VASLKAYIGALNKMLGF 78
           V+S+KAY+GALNKM+ F
Sbjct: 598 VSSVKAYVGALNKMMDF 614

The following BLAST results are available for this feature:

BLAST of CmaCh01G003520 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C550	5.5e-28	77.92	2-isopropylmalate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=I...	[more]
Q9LPR4	4.7e-27	76.62	2-isopropylmalate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=I...	[more]
O04974	1.6e-22	67.57	2-isopropylmalate synthase B OS=Solanum pennellii OX=28526 GN=IPMSB PE=2 SV=1	[more]
O04973	2.3e-18	59.46	2-isopropylmalate synthase A OS=Solanum pennellii OX=28526 GN=IPMSA PE=2 SV=1	[more]
Q39891	1.7e-13	53.33	Probable 2-isopropylmalate synthase OS=Glycine max OX=3847 GN=GMN56 PE=2 SV=1	[more]

BLAST of CmaCh01G003520 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K8I4	4.7e-46	100.00	2-isopropylmalate synthase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1G9D1	4.7e-38	95.51	2-isopropylmalate synthase OS=Cucurbita moschata OX=3662 GN=LOC111452159 PE=3 SV...	[more]
A0A6J1CP08	6.6e-32	81.11	2-isopropylmalate synthase 2, chloroplastic-like OS=Momordica charantia OX=3673 ...	[more]
A0A6J1CKJ3	8.6e-32	81.11	2-isopropylmalate synthase OS=Momordica charantia OX=3673 GN=LOC111012138 PE=3 S...	[more]
A0A0A0L7C8	9.5e-31	77.78	2-isopropylmalate synthase OS=Cucumis sativus OX=3659 GN=Csa_3G363180 PE=3 SV=1	[more]

BLAST of CmaCh01G003520 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022998632.1	9.7e-46	100.00	2-isopropylmalate synthase 2, chloroplastic-like [Cucurbita maxima]	[more]
XP_022948501.1	9.7e-38	95.51	2-isopropylmalate synthase 2, chloroplastic-like [Cucurbita moschata]	[more]
KAG6607000.1	9.7e-38	95.51	2-isopropylmalate synthase 2, chloroplastic, partial [Cucurbita argyrosperma sub...	[more]
KAG7036701.1	9.7e-38	95.51	2-isopropylmalate synthase 2, chloroplastic, partial [Cucurbita argyrosperma sub...	[more]
XP_023522725.1	2.8e-37	94.38	2-isopropylmalate synthase 2, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmaCh01G003520 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G74040.1	3.9e-29	77.92	2-isopropylmalate synthase 1	[more]
AT1G18500.1	3.3e-28	76.62	methylthioalkylmalate synthase-like 4	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036230	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain superfamily	GENE3D	3.30.160.270		coord: 1..75 e-value: 1.4E-15 score: 59.3
IPR036230	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain superfamily	SUPERFAMILY	110921	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain	coord: 4..74
IPR013709	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain	PFAM	PF08502	LeuA_dimer	coord: 2..75 e-value: 1.7E-14 score: 53.8
None	No IPR available	PANTHER	PTHR10277:SF65	2-ISOPROPYLMALATE SYNTHASE A-LIKE ISOFORM X1	coord: 3..78
None	No IPR available	PANTHER	PTHR10277	HOMOCITRATE SYNTHASE-RELATED	coord: 3..78

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh01G003520.1	CmaCh01G003520.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009098	leucine biosynthetic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0003852	2-isopropylmalate synthase activity