CmaCh01G003510 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh01G003510
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	2-isopropylmalate synthase
Location	Cma_Chr01: 1739266 .. 1744740 (+)
RNA-Seq Expression	CmaCh01G003510
Synteny	CmaCh01G003510

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CGTCAATCCGACGTTGATTCTCTCTGACTTCGATCCTCCATGCATGCGAAGCTTACTATCTTGTCGGTGTGAAATCTGTCCGCATTTCCGCTTGAAAAGCTCCCACGAATGTTCGCTGGAGTTCGGCTGTAAGTATTCTCTGTTCTTCATTCATTGCCGATCAGTTGCTTCCTCAATTGCGCGATAAATAGAAGGAGAAGCTTGTTTGATTTGTTAGAGTATTTCTGTTTTGTTTGATTTGCGGTTGTTGTGTTTATCATGGCCGTGCCGGTAACTTTGTCGAGTCCGAATCACTTTTTTGTTCGGCATTCGAACTCTCAGTTTCCTTCCTGTCATCGGAGTTACAATCGGAATTTGCAGGCTCTTAAGAATTCTTCATGGTCGTTGTCCTTTAGAACGGTGGCGGACAATGTGTCTCGCATTTACTGCAGTCGCACTGAAAGCGTAGTTTCGAATTCCAAATCGAATTCATCCATTAGACGTCCGCCGTATATTCCTAATCGCATCCCTGACTCTTCCTATGTTCGGATATTTGATACCACTCTCCGCGATGGCGAGCAGTCTCCAGGTGCCTCCCTCACTGCTAAAGAGAAGCTTGATATTGCTCGTCAGCTCGTCAAGCTTGGCGTTGACATAATTGAGGCTGGATTTCCTGCTGCTTCGAAGGAAGACTTTGAGGCCGTCAAGATGATTGCCGCAGAGGTTGGCAATGCTGTCGATGAGGATGGGTATGTGCCTGTTATTTGTGGTCTTTCGAGGTGTAATGAGAATGATATTCGAACTGCATGGGAGGCTGTGAAGTACGCTAAGAGACCGCGGATACATACGTTTATAGCAACAAGCGAGATTCATATGGAGCATAAGCTGAGAAAGACGAGGGAGCAAGTGATTGATATTGCTCGAAATATGGTGCGGTTTGCACAAAGTTTGGGTTGTGATGATGTTGAGTTTAGCCCCGAGGATGCTGGGAGGTTCAATCTTGATCTCAATTTACATGCATTTTCTAATTTTTGTTTCAATGATTGGTCCTGAGAGTAAGTTGACTTTTCTTGATGATTTCAAAACCTCTCCATCATAATGGTATTTTGGATTAAGTTGTTAATAAGTTCAGATTTATGAAGCTTGAAGTTGTGAAGTGGGAATTTATGTAATTTGTTATTGTATCCCGTAAAAGAATAGGATGTAAGATATGTAAATCTTTGTATTTTGTTTGTTTTCCTGATGCTTGATTTATGTAATTTGGATTTGCGTAGCTAATGGTTTGCAATTTTTGATTAGCAGAATCATATGCTAGTAGCCTTCTTACTGTTCATTGTGTAATCACTGACTGATGTGTCTATCCCCATCCTTGTCTTGACGAGAAAAGATCCTTCATAGGGACCCTTCTCTATGGGGATTTCGACATCCTTAGCCATCGTTGCTTAAAATAAAATTGCAGTTCTTTTAAGTTGATAAATTAGTTTTCTTTTTTATGTTGTGGTGTTCTATCAGATCTGACAGAGAGTTCCTTTATCAAATTCTGGGCGAAGTGATAAAGGCTGGTGCAACTACTCTCAACATACCAGATACTGTGGGTTATACAATGCCAGTTGAATTTGGCAACTTGATTGCTGATTTAAAAGCTAATACTCCTGGCATTGATAATGTTATCATCTCAACACACTGCCAGAATGATCTTGGACTCGCAACTTCCAACACAGTAGCAGTAAGTAATGGCATTTTCCTCCCTTTTAATCCTATTTATGACGTGTTTGGGAATGTGGCCAAAATCACCTTTTTCATGCCCAAGAACTCTAAACAGGGGATGAAATTCCAAAACCACTCCTAAACATGTCTTTATTTATTGAATTAAATTAGGTCTGTAATTTTGGTAAGTATCCGAATATAATTGGAAAGTTACAAGGATTATGAATAACATACCATTAACACTAAGCATGATATAGAGAATTAACTTCGTAATCAATACCCAAATTATGATTCTTCTCATCTCTTTGCAGGGGGCATGTGCAGGCGCAAGGCAACTTGAGGTCACAATTAATGGCATTGGTGAGAGAGCTGGAAATGCTTCGCTAGAGGAGGCAAGTCAATTATTTATTTTTTTCTGTGTTTATTCATATTATTATTATTCATATTTTTATTTTGTGAAGAGCGACGATGACTTGCAATGCAACCTAGCTTAATTGGTAATCGATATGTCGTACTTATTTCATACTATGTTTTTTATTTACAAAAGAGGTTAAATTTTAAATTCTGAGAAATTAAGAATGTTTATATATTTCATTTTACATGCGACTGTATGCATGTTTCTGTTGTATTGTTCTAAGAACATCTGTTTGTGCAGACATCAAAACATTCATATTTCGTGGGTAGACTTCTTAAATGGCATGTATGAATGTACACAATTTTCATTTTTTATACCATATTTTTAAATCTTCAGGTGGTAATGGCCTTACAATGTCGTGGAGAGCATGTGCTTGGAGGTCTTCGTACTGGAATTAACTCAAGACATATCTTTTTGACTAGCAAGATGGTAATTACTTCTCTTACAAAAAATGCTTCTTGAAAAAAAAATTCCTTGAGTTATTTTTTTTTTATTATTCTTTTGGATGCTAGACAGTTCTGTTTCGCTTCTGGTTCCTTGGAAAGAGTCGAGGCAATTTTTTGAGGATAGAGATAGGAGCATTTATTTCATTTTTTTAGGGGTAGGACTGGTACTTTGAGAGATGTTTAATGTTACTAATTAGGTATGAATGTCCTAACTTCTATTTATTTTATTTCTCTTATGACTACTGTTTCCTTTTAAAATCTGCTGGTTGGACTTTTTCCTCCTTTGAGGCGAAGTATATTTGGGAATTTGGATTAAGGACAGATTTGGTAGCTCTCCCTTTCCCCCAGTTCTCTTTTATGTTTTTTGTATTAAATATCTCTATTTGTTATCAATAAAGGCTACTTATTGATTAACTCAATCCTTAGGTAGAAGAGTACACTGGTTTGAATGTACAACCACGCAAGGCAATTGTGGGAGCTAATGCTTTTGCTCATGAAAGTGGCATCCACCAGGTTTGTCATGTTCAATTTTGATTTTTGCTATTCGCAGCTAACTATTTATGACACATTCTCATATTTATCTTTCGAAACCTATCGTGATTTGAAGTCTACCCTATATGCTTATAGTTGACTATTACAGAATTCTATCACCTAGGGATGTACATCCAACCCGACAATCCTAACCAACTCAACCCGAATTATAGGGGTTGGGTTGCTTTTTCTGAACTCAGAGTGACTCGGTTCGGTTTTAGGTTCATAGGATAAAAAACTCGGGTGGACCCGAAATCGAACAAAACTTATAAATTACACTAAGAATTCTTGCCTATGTAAAGGTCTATCAGAGTGTCAATCCGAGTTTATTAGAATACTCTCGTTTGCTCAAGCCAAAATCATCTTTTTATTATTTTGTTTGTTTCCTTTATTTATTTATTTTTTTTGTTTCTTTTATATTATTCTATTTAATTTCTTTTATATATTTTAAAATTCTCTACTAAATATATAAAAATACTTCAATATTTCATACTATTTTTAGACACATTATTTATTGTTTTTTAATTTTTGTTGTTTCTTTTATATTATTTTATTTAATTTCTTTTATATATTTTAAAATTCTCTTCTAAATATATAAAAATACCTCAATATTTCATACTATTTTTAAACACATTATTATAACGTTAACTAATCATTTTTATTGTTTTTTAATTTCCTAATCTATCCTTATATTTTCTTTGTAACCAATTATTGTTGAACAAGAACTTACAACTTTTCCTTATTATGATTTTTTTTTTAATTATAGATTGATTTGGGTTTGCTTGTAGACTTTTAATATTTTTTTTATCTAAGATTATACCGAATGATTATAAATTCGGGTTGGGTTGGGTTACCAATCCAACCAACCCGAACTTTTGGGATCGGTCAAAAAAAATCTTTCAACCCAACTCAACTTGGACCATGTACACTCCTATTAGCACCAGTATTTTTTGTTTCCAACACATTTATGGCAAACAATTACTCATTTTTAACTTTAGTTCCCCTTTTCTTGCGAATTAATTTTTGGATAATGAAATTCAGAAAACAAAGTTAGGAAGGATGGTAATGGAAGATGAGTGTTTCCTACGAGGTGCTGAAGACTATACTGAATTCTCCCCAATTTGGTAGATTGGAAAAGAAGTGTGAAAAACCTTATCTTAAGCCCTTCAAGAGGAAGGAAGAAAGTTGTTAAACTGCTGCTAAGTTCTCTTACACATATCGAGAACTCTTTTAAACTATTTTAATGTATAGTCTTGCTTTCAAGCTCATTATGAAAATGTTGATTGGCTCTTATTTCTATGTTTTGAGTTATTATTATAATTATTATTATTTGTGTGAGACATGCGGTCGGCTGTTTGCCCTGCTTTGTTAGGATCCAATTGTATCTTGAACATTGCTTTTGTGGTGCAGGATGGGATGCTTAAGCACAAAGGTACTTATGAAATCATATCCCCTGAAGTCATTGGCTACGAACAATCCAAAATGATGCTGGTATTGTTCTAGGAAAACTCAGGTACACCATGTTGCTTTATGGAAAATGACGATATTGCCTTTCTCTCGATAAAATTTCCCCCTCTCCTGGTCTCCTCTTCCCCATCTCTATGTGACTCTACAATATTTGATTTTCGAGGTAACTCATACAATATTAAATCCTCCTAGTGATTACAATGACTTGATGAACCCATTCCTATTTTCCGTTGAAGGTCAGATTAACTTCCAAAATATTTAGAATAGACATCGAAAAATTGTTCTCTTTAGATCTAAATCTTCTTATAGCTGGGTTCAACAATGCAACAGTACGAAGGAACTAAATAGTTTTTCAGGGACAGAAGAAATGACAAACATCTGTTGTGGTTCTGTTGTAGTGCTGCCCAAAATCATTTTTTTACAATATTAACAGGATTGACTTTATATGTTTATTTCACTTTCTGTGTCTTGTTCAAAGTATACTATCCCAATAACATCTTCATTATGACTACGTTTACAGTGGACGCCATGCCCTTAAAAGTCGACTTCTAGAGGTAAATTTAGATCTCCAACCTCAATGTTTAGGTAGTATTTCCATTTTATAATAGGCTAATGTTATTTGTCTTTTCAGCTTGGTTATGAGCTTGACAATGAGAATCTTGACAACGTATTCTGGCGTTTCAAAGCAGTAGCTGAACAAAAGAAGGTAACTCAAAATTTCGTCCCTCTCTTTTGCTGAGCTATTCCCTATCCTGCTTCCTACACATAGTAGAGTAGCAAATACCATTTGGGATAATTCAGCCTGTTTCAAGAACATAAATAACTACTCATCAAGTTAGTTTTTCATTTAACCTGTTCTAAACTTTTTGCCATAATTTATCTTCAACATTCCAGAGAGTTACAGACAGATCTTCGAGCGTTGGTATCTGA

mRNA sequence

CGTCAATCCGACGTTGATTCTCTCTGACTTCGATCCTCCATGCATGCGAAGCTTACTATCTTGTCGGTGTGAAATCTGTCCGCATTTCCGCTTGAAAAGCTCCCACGAATGTTCGCTGGAGTTCGGCTGTAAGTATTCTCTGTTCTTCATTCATTGCCGATCAGTTGCTTCCTCAATTGCGCGATAAATAGAAGGAGAAGCTTGTTTGATTTGTTAGAGTATTTCTGTTTTGTTTGATTTGCGGTTGTTGTGTTTATCATGGCCGTGCCGGTAACTTTGTCGAGTCCGAATCACTTTTTTGTTCGGCATTCGAACTCTCAGTTTCCTTCCTGTCATCGGAGTTACAATCGGAATTTGCAGGCTCTTAAGAATTCTTCATGGTCGTTGTCCTTTAGAACGGTGGCGGACAATGTGTCTCGCATTTACTGCAGTCGCACTGAAAGCGTAGTTTCGAATTCCAAATCGAATTCATCCATTAGACGTCCGCCGTATATTCCTAATCGCATCCCTGACTCTTCCTATGTTCGGATATTTGATACCACTCTCCGCGATGGCGAGCAGTCTCCAGGTGCCTCCCTCACTGCTAAAGAGAAGCTTGATATTGCTCGTCAGCTCGTCAAGCTTGGCGTTGACATAATTGAGGCTGGATTTCCTGCTGCTTCGAAGGAAGACTTTGAGGCCGTCAAGATGATTGCCGCAGAGGTTGGCAATGCTGTCGATGAGGATGGGTATGTGCCTGTTATTTGTGGTCTTTCGAGGTGTAATGAGAATGATATTCGAACTGCATGGGAGGCTGTGAAGTACGCTAAGAGACCGCGGATACATACGTTTATAGCAACAAGCGAGATTCATATGGAGCATAAGCTGAGAAAGACGAGGGAGCAAGTGATTGATATTGCTCGAAATATGGTGCGGTTTGCACAAAGTTTGGGTTGTGATGATGTTGAGTTTAGCCCCGAGGATGCTGGGAGATCTGACAGAGAGTTCCTTTATCAAATTCTGGGCGAAGTGATAAAGGCTGGTGCAACTACTCTCAACATACCAGATACTGTGGGTTATACAATGCCAGTTGAATTTGGCAACTTGATTGCTGATTTAAAAGCTAATACTCCTGGCATTGATAATGTTATCATCTCAACACACTGCCAGAATGATCTTGGACTCGCAACTTCCAACACAGTAGCAGGGGCATGTGCAGGCGCAAGGCAACTTGAGGTCACAATTAATGGCATTGGTGAGAGAGCTGGAAATGCTTCGCTAGAGGAGGTGGTAATGGCCTTACAATGTCGTGGAGAGCATGTGCTTGGAGGTCTTCGTACTGGAATTAACTCAAGACATATCTTTTTGACTAGCAAGATGGTAGAAGAGTACACTGGTTTGAATGTACAACCACGCAAGGCAATTGTGGGAGCTAATGCTTTTGCTCATGAAAGTGGCATCCACCAGGATGGGATGCTTAAGCACAAAGGAAAACTCAGTGGACGCCATGCCCTTAAAAGTCGACTTCTAGAGCTTGGTTATGAGCTTGACAATGAGAATCTTGACAACGTATTCTGGCGTTTCAAAGCAGTAGCTGAACAAAAGAAGAGAGTTACAGACAGATCTTCGAGCGTTGGTATCTGA

Coding sequence (CDS)

ATGGCCGTGCCGGTAACTTTGTCGAGTCCGAATCACTTTTTTGTTCGGCATTCGAACTCTCAGTTTCCTTCCTGTCATCGGAGTTACAATCGGAATTTGCAGGCTCTTAAGAATTCTTCATGGTCGTTGTCCTTTAGAACGGTGGCGGACAATGTGTCTCGCATTTACTGCAGTCGCACTGAAAGCGTAGTTTCGAATTCCAAATCGAATTCATCCATTAGACGTCCGCCGTATATTCCTAATCGCATCCCTGACTCTTCCTATGTTCGGATATTTGATACCACTCTCCGCGATGGCGAGCAGTCTCCAGGTGCCTCCCTCACTGCTAAAGAGAAGCTTGATATTGCTCGTCAGCTCGTCAAGCTTGGCGTTGACATAATTGAGGCTGGATTTCCTGCTGCTTCGAAGGAAGACTTTGAGGCCGTCAAGATGATTGCCGCAGAGGTTGGCAATGCTGTCGATGAGGATGGGTATGTGCCTGTTATTTGTGGTCTTTCGAGGTGTAATGAGAATGATATTCGAACTGCATGGGAGGCTGTGAAGTACGCTAAGAGACCGCGGATACATACGTTTATAGCAACAAGCGAGATTCATATGGAGCATAAGCTGAGAAAGACGAGGGAGCAAGTGATTGATATTGCTCGAAATATGGTGCGGTTTGCACAAAGTTTGGGTTGTGATGATGTTGAGTTTAGCCCCGAGGATGCTGGGAGATCTGACAGAGAGTTCCTTTATCAAATTCTGGGCGAAGTGATAAAGGCTGGTGCAACTACTCTCAACATACCAGATACTGTGGGTTATACAATGCCAGTTGAATTTGGCAACTTGATTGCTGATTTAAAAGCTAATACTCCTGGCATTGATAATGTTATCATCTCAACACACTGCCAGAATGATCTTGGACTCGCAACTTCCAACACAGTAGCAGGGGCATGTGCAGGCGCAAGGCAACTTGAGGTCACAATTAATGGCATTGGTGAGAGAGCTGGAAATGCTTCGCTAGAGGAGGTGGTAATGGCCTTACAATGTCGTGGAGAGCATGTGCTTGGAGGTCTTCGTACTGGAATTAACTCAAGACATATCTTTTTGACTAGCAAGATGGTAGAAGAGTACACTGGTTTGAATGTACAACCACGCAAGGCAATTGTGGGAGCTAATGCTTTTGCTCATGAAAGTGGCATCCACCAGGATGGGATGCTTAAGCACAAAGGAAAACTCAGTGGACGCCATGCCCTTAAAAGTCGACTTCTAGAGCTTGGTTATGAGCTTGACAATGAGAATCTTGACAACGTATTCTGGCGTTTCAAAGCAGTAGCTGAACAAAAGAAGAGAGTTACAGACAGATCTTCGAGCGTTGGTATCTGA

Protein sequence

MAVPVTLSSPNHFFVRHSNSQFPSCHRSYNRNLQALKNSSWSLSFRTVADNVSRIYCSRTESVVSNSKSNSSIRRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKLDIARQLVKLGVDIIEAGFPAASKEDFEAVKMIAAEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIDIARNMVRFAQSLGCDDVEFSPEDAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADLKANTPGIDNVIISTHCQNDLGLATSNTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRHIFLTSKMVEEYTGLNVQPRKAIVGANAFAHESGIHQDGMLKHKGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTDRSSSVGI

Homology

[Close]

BLAST of CmaCh01G003510 vs. ExPASy Swiss-Prot
Match: Q9C550 (2-isopropylmalate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPMS2 PE=1 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 3.8e-177
Identity = 311/397 (78.34%), Postives = 348/397 (87.66%), Query Frame = 0


Query: 74  RRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKLDIARQLVKLGVDIIEAGFPA 133
           RRP YIPNRI D +YVRIFDTTLRDGEQSPGA+LT+KEKLDIARQL KLGVDIIEAGFPA
Sbjct: 72  RRPNYIPNRISDPNYVRIFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPA 131

Query: 134 ASKEDFEAVKMIAAEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTFIA 193
           ASK+DFEAVK IA  VGN VDE+GYVPVICGLSRCN+ DI TAWEAVKYAKRPRIHTFIA
Sbjct: 132 ASKDDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIETAWEAVKYAKRPRIHTFIA 191

Query: 194 TSEIHMEHKLRKTREQVIDIARNMVRFAQSLGCDDVEFSPEDAGRSDREFLYQILGEVIK 253
           TS+IH+++KL+K++E+VI+IARNMVRFA+SLGC+DVEFSPEDAGRS+RE+LY+ILGEVIK
Sbjct: 192 TSDIHLKYKLKKSKEEVIEIARNMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVIK 251

Query: 254 AGATTLNIPDTVGYTMPVEFGNLIADLKANTPGIDNVIISTHCQNDLGLATSNTVAGACA 313
           AGATTLNIPDTVG T+P EFG LIAD+KANTPGI NVIISTHCQNDLGL+T+NT++GA +
Sbjct: 252 AGATTLNIPDTVGITLPSEFGQLIADIKANTPGIQNVIISTHCQNDLGLSTANTLSGAHS 311

Query: 314 GARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRHIFLTSKMVEEYTG 373
           GARQ+EVTINGIGERAGNASLEEVVMA++CRG+HVLGGL TGI++RHI +TSKMVEEYTG
Sbjct: 312 GARQVEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTG 371

Query: 374 LNVQPRKAIVGANAFAHESGIHQDGMLKHK-----------------------GKLSGRH 433
           +  QP KAIVGANAFAHESGIHQDGMLKHK                       GKLSGRH
Sbjct: 372 MQTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIMSPEEIGLERSNDAGIVLGKLSGRH 431

Query: 434 ALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD 448
           ALK RL ELGY LD+  L N+FWRFKAVAEQKKRVTD
Sbjct: 432 ALKDRLNELGYVLDDGQLSNLFWRFKAVAEQKKRVTD 468

[Close]

BLAST of CmaCh01G003510 vs. ExPASy Swiss-Prot
Match: Q9LPR4 (2-isopropylmalate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPMS1 PE=1 SV=2)

HSP 1 Score: 619.0 bits (1595), Expect = 4.2e-176
Identity = 306/396 (77.27%), Postives = 345/396 (87.12%), Query Frame = 0


Query: 75  RPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKLDIARQLVKLGVDIIEAGFPAA 134
           RP YIPNRI D +YVR+FDTTLRDGEQSPGA+LT+KEKLDIARQL KLGVDIIEAGFPAA
Sbjct: 75  RPEYIPNRISDPNYVRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAA 134

Query: 135 SKEDFEAVKMIAAEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTFIAT 194
           SK+DFEAVK IA  VGN VDE+GYVPVICGLSRCN+ DI  AW+AVKYAKRPRIHTFIAT
Sbjct: 135 SKDDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIERAWDAVKYAKRPRIHTFIAT 194

Query: 195 SEIHMEHKLRKTREQVIDIARNMVRFAQSLGCDDVEFSPEDAGRSDREFLYQILGEVIKA 254
           S+IH+E+KL+KT+ +VI+IAR+MVRFA+SLGC+DVEFSPEDAGRS+RE+LY+ILGEVIKA
Sbjct: 195 SDIHLEYKLKKTKAEVIEIARSMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKA 254

Query: 255 GATTLNIPDTVGYTMPVEFGNLIADLKANTPGIDNVIISTHCQNDLGLATSNTVAGACAG 314
           GATTLNIPDTVG T+P EFG LI DLKANTPGI+NV+ISTHCQNDLGL+T+NT++GA AG
Sbjct: 255 GATTLNIPDTVGITLPSEFGQLITDLKANTPGIENVVISTHCQNDLGLSTANTLSGAHAG 314

Query: 315 ARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRHIFLTSKMVEEYTGL 374
           ARQ+EVTINGIGERAGNASLEEVVMA++CRG+HVLGGL TGI++RHI +TSKMVEEYTG+
Sbjct: 315 ARQMEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGM 374

Query: 375 NVQPRKAIVGANAFAHESGIHQDGMLKHK-----------------------GKLSGRHA 434
             QP KAIVGANAFAHESGIHQDGMLKHK                       GKLSGRHA
Sbjct: 375 QTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEEIGLERSNDAGIVLGKLSGRHA 434

Query: 435 LKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD 448
           LK RL ELGY+LD+E L  +FWRFK VAEQKKRVTD
Sbjct: 435 LKDRLTELGYQLDDEQLSTIFWRFKTVAEQKKRVTD 470

[Close]

BLAST of CmaCh01G003510 vs. ExPASy Swiss-Prot
Match: O04973 (2-isopropylmalate synthase A OS=Solanum pennellii OX=28526 GN=IPMSA PE=2 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 4.4e-173
Identity = 298/399 (74.69%), Postives = 348/399 (87.22%), Query Frame = 0


Query: 72  SIRRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKLDIARQLVKLGVDIIEAGF 131
           S RRP Y+P++I D  YVRIFDTTLRDGEQSPGA++T KEKLD+ARQL KLGVDIIEAGF
Sbjct: 31  SNRRPEYVPSKISDPKYVRIFDTTLRDGEQSPGATMTTKEKLDVARQLAKLGVDIIEAGF 90

Query: 132 PAASKEDFEAVKMIAAEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTF 191
           PA+S+ DFE+VK+IA E+GN  DE+G+VPVICGLSRCN++DI  AWEAVKYAK+PR+HTF
Sbjct: 91  PASSEADFESVKLIAEEIGNNTDENGFVPVICGLSRCNKSDIDKAWEAVKYAKKPRVHTF 150

Query: 192 IATSEIHMEHKLRKTREQVIDIARNMVRFAQSLGCDDVEFSPEDAGRSDREFLYQILGEV 251
           IATSEIHM++KL+ +REQV++ AR+MV +A+SLGC+DVEFSPEDAGRSDREFLY ILGEV
Sbjct: 151 IATSEIHMKYKLKMSREQVVEKARSMVAYARSLGCEDVEFSPEDAGRSDREFLYDILGEV 210

Query: 252 IKAGATTLNIPDTVGYTMPVEFGNLIADLKANTPGIDNVIISTHCQNDLGLATSNTVAGA 311
           IKAGATTLNIPDTVGYT+P EFG LI D+KANTPGI+NVIISTHCQNDLGL+T+NT+AGA
Sbjct: 211 IKAGATTLNIPDTVGYTVPSEFGQLITDIKANTPGIENVIISTHCQNDLGLSTANTLAGA 270

Query: 312 CAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRHIFLTSKMVEEY 371
           CAGARQLEVTINGIGERAGNASLEEVVMAL+CRGE VLGGL TGIN++HI  +SKMVEEY
Sbjct: 271 CAGARQLEVTINGIGERAGNASLEEVVMALKCRGEQVLGGLYTGINTQHIVPSSKMVEEY 330

Query: 372 TGLNVQPRKAIVGANAFAHESGIHQDGMLKHK-----------------------GKLSG 431
           +GL VQP KAIVGANAFAHESGIHQDGMLKHK                       GKLSG
Sbjct: 331 SGLQVQPHKAIVGANAFAHESGIHQDGMLKHKDTYEIISPDDVGLSRSNEAGIVLGKLSG 390

Query: 432 RHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD 448
           RHALKS++LELGY++D + L+++FWRFK+VAE+KK++TD
Sbjct: 391 RHALKSKMLELGYDIDGKELEDLFWRFKSVAEKKKKITD 429

[Close]

BLAST of CmaCh01G003510 vs. ExPASy Swiss-Prot
Match: O04974 (2-isopropylmalate synthase B OS=Solanum pennellii OX=28526 GN=IPMSB PE=2 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 2.4e-163
Identity = 287/400 (71.75%), Postives = 337/400 (84.25%), Query Frame = 0


Query: 72  SIRR-PPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKLDIARQLVKLGVDIIEAG 131
           SIRR P Y P+ IPD +YVRIFDTTLRDGEQSPGA++T KEKLD+ARQ  KLGVDIIEAG
Sbjct: 53  SIRRIPEYTPSHIPDPNYVRIFDTTLRDGEQSPGATMTTKEKLDVARQSAKLGVDIIEAG 112

Query: 132 FPAASKEDFEAVKMIAAEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHT 191
           FPA+S+ D EAVK+IA EVGN V E+ YVPVICGL+RCN+ DI  AWEAVKYAK+PRIHT
Sbjct: 113 FPASSEADLEAVKLIAKEVGNGVYEEEYVPVICGLARCNKKDIDKAWEAVKYAKKPRIHT 172

Query: 192 FIATSEIHMEHKLRKTREQVIDIARNMVRFAQSLGCDDVEFSPEDAGRSDREFLYQILGE 251
           FIATSE+HM +KL+ +R+QV++ AR+MV +A+S+GC+DVEFSPEDAGRSD EFLY ILGE
Sbjct: 173 FIATSEVHMNYKLKMSRDQVVEKARSMVAYARSIGCEDVEFSPEDAGRSDPEFLYHILGE 232

Query: 252 VIKAGATTLNIPDTVGYTMPVEFGNLIADLKANTPGIDNVIISTHCQNDLGLATSNTVAG 311
           VIKAGATTLNIPDTVGYT+P EFG LIA +KANTPG+++VIISTHCQNDLGL+T+NT+AG
Sbjct: 233 VIKAGATTLNIPDTVGYTVPEEFGQLIAKIKANTPGVEDVIISTHCQNDLGLSTANTLAG 292

Query: 312 ACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRHIFLTSKMVEE 371
           ACAGARQLEVTINGIGERAGNASLEEVVMAL+CRGE VLGGL TGIN++HI ++SKMVE 
Sbjct: 293 ACAGARQLEVTINGIGERAGNASLEEVVMALKCRGEQVLGGLYTGINTQHILMSSKMVEG 352

Query: 372 YTGLNVQPRKAIVGANAFAHESGIHQDGMLKHK-----------------------GKLS 431
            +GL+VQP KAIVGANAF HESGIHQDGMLKHK                       GKLS
Sbjct: 353 ISGLHVQPHKAIVGANAFVHESGIHQDGMLKHKDTYEIISPEDIGLNRANESGIVFGKLS 412

Query: 432 GRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD 448
           G    K ++LELGYE++ + LD++FWRFK+VAE+KK++TD
Sbjct: 413 GVMLCKPKMLELGYEIEGKELDDLFWRFKSVAEKKKKITD 452

[Close]

BLAST of CmaCh01G003510 vs. ExPASy Swiss-Prot
Match: Q39891 (Probable 2-isopropylmalate synthase OS=Glycine max OX=3847 GN=GMN56 PE=2 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 6.4e-156
Identity = 284/409 (69.44%), Postives = 323/409 (78.97%), Query Frame = 0


Query: 62  SVVSNSKSNSSIRRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKLDIARQLVK 121
           S  S+     S  RP YIPN IPDSSYVRI DTTLRDGEQSPGA++TAKEKLDIARQLVK
Sbjct: 6   STPSSQSPKLSHLRPQYIPNHIPDSSYVRILDTTLRDGEQSPGATMTAKEKLDIARQLVK 65

Query: 122 LGVDIIEAGFPAASKEDFEAVKMIAAEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAVK 181
           LGVDII+ GFP+AS  DF AVKMIA EVGNAVD+DGYVPVI G  RC E DI TAWEAVK
Sbjct: 66  LGVDIIQPGFPSASNSDFMAVKMIAQEVGNAVDDDGYVPVIAGFCRCVEKDISTAWEAVK 125

Query: 182 YAKRPRIHTFIATSEIHMEHKLRKTREQVIDIARNMVRFAQSLGCDDVEFSPEDAGRSDR 241
           YAKRPR+ T IATS IHMEHKLRK+++QVI IAR+MV+FA+SLGC+D++F  EDA RSDR
Sbjct: 126 YAKRPRLCTSIATSPIHMEHKLRKSKDQVIQIARDMVKFARSLGCNDIQFGAEDATRSDR 185

Query: 242 EFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADLKANTPGIDNVIISTHCQNDLG 301
           EFLY+ILG VI+AGATT+NI DTVG  MP+E G LI D+K NTPGI NVIISTHC NDLG
Sbjct: 186 EFLYEILGVVIEAGATTVNIADTVGIVMPLELGKLIVDIKDNTPGIANVIISTHCHNDLG 245

Query: 302 LATSNTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRHI 361
           LAT+NT+ GA  GARQLEVTINGIGERAGNASLEEVVMAL  +G+H L GL T IN+RHI
Sbjct: 246 LATANTIEGARTGARQLEVTINGIGERAGNASLEEVVMALASKGDHALNGLYTRINTRHI 305

Query: 362 FLTSKMVEEYTGLNVQPRKAIVGANAFAHESGIHQDGMLKHK------------------ 421
             TSKMVEEY+G+++QP K +VGANAF H SGIHQDGMLKHK                  
Sbjct: 306 LETSKMVEEYSGMHLQPHKPLVGANAFVHASGIHQDGMLKHKGTYETISPEEIGHKRTTR 365

Query: 422 -----GKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD 448
                GKLSG  AL+ RL ELGY+L  + +D+VFW+FKA+AE+KK VTD
Sbjct: 366 IGIVLGKLSGSQALRKRLEELGYDLKEDEVDSVFWQFKAMAEKKKVVTD 414

[Close]

BLAST of CmaCh01G003510 vs. ExPASy TrEMBL
Match: A0A6J1G9D1 (2-isopropylmalate synthase OS=Cucurbita moschata OX=3662 GN=LOC111452159 PE=3 SV=1)

HSP 1 Score: 851.3 bits (2198), Expect = 1.9e-243
Identity = 435/470 (92.55%), Postives = 442/470 (94.04%), Query Frame = 0


Query: 1   MAVPVTLSSPNHFFVRHSNSQFPSCHRSYNRNLQALKNSSWSLSFRTVADNVSRIYCSRT 60
           MAVPVTLSSPNHF VRHSNSQFPSCHR YNRNLQALKNSS SLSFRTVADNVSRIYCS T
Sbjct: 1   MAVPVTLSSPNHFLVRHSNSQFPSCHRRYNRNLQALKNSSRSLSFRTVADNVSRIYCSHT 60

Query: 61  ESVVSNSKSNSSIRRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKLDIARQLV 120
           ESVVSNSKSNSSI+RPPYIPNRIPDSSYVR+FDTTLRDGEQSPGASLTAKEKLDIARQLV
Sbjct: 61  ESVVSNSKSNSSIKRPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIARQLV 120

Query: 121 KLGVDIIEAGFPAASKEDFEAVKMIAAEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAV 180
           KLGVDIIEAGFPAASKEDFEAVKMIAAEVGNAV+EDGYVPVICGLSRCNENDIRTAWEAV
Sbjct: 121 KLGVDIIEAGFPAASKEDFEAVKMIAAEVGNAVNEDGYVPVICGLSRCNENDIRTAWEAV 180

Query: 181 KYAKRPRIHTFIATSEIHMEHKLRKTREQVIDIARNMVRFAQSLGCDDVEFSPEDAGRSD 240
           KYAKRPRIHTFIATSEIHMEHKLRKTREQVI+IARNMVRFA+SLGCDDVEFSPEDAGRSD
Sbjct: 181 KYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDAGRSD 240

Query: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADLKANTPGIDNVIISTHCQNDL 300
           REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIAD+KANTPGIDNVIISTHCQNDL
Sbjct: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHCQNDL 300

Query: 301 GLATSNTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360
           GLAT+NTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH
Sbjct: 301 GLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360

Query: 361 IFLTSKMVEEYTGLNVQPRKAIVGANAFAHESGIHQDGMLKHK----------------- 420
           IFLTSKMVEEYTGLNVQP KAIVGANAFAHESGIHQDGMLKHK                 
Sbjct: 361 IFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSN 420

Query: 421 ------GKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD 448
                 GKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD
Sbjct: 421 DAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD 470

[Close]

BLAST of CmaCh01G003510 vs. ExPASy TrEMBL
Match: A0A6J1J7V5 (2-isopropylmalate synthase OS=Cucurbita maxima OX=3661 GN=LOC111482524 PE=3 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 1.6e-226
Identity = 406/470 (86.38%), Postives = 424/470 (90.21%), Query Frame = 0


Query: 1   MAVPVTLSSPNHFFVRHSNSQFPSCHRSYNRNLQALKNSSWSLSFRTVADNVSRIYCSRT 60
           MA+P   S PN F VR  NSQF + HR Y+RNLQALKNSS SLSFRT AD+VSRIYC + 
Sbjct: 1   MAMPAAFSRPNQFLVRQPNSQFSARHRIYSRNLQALKNSSRSLSFRTAADHVSRIYCCQK 60

Query: 61  ESVVSNSKSNSSIRRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKLDIARQLV 120
           ES+VSNS+S SSIRRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEK+DIARQL 
Sbjct: 61  ESIVSNSESKSSIRRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKVDIARQLT 120

Query: 121 KLGVDIIEAGFPAASKEDFEAVKMIAAEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAV 180
           KLGVDIIEAGFPAASKEDFEAVKMIA EVGNAVDEDGYVPVICGLSRCNE DIRTAWEAV
Sbjct: 121 KLGVDIIEAGFPAASKEDFEAVKMIAKEVGNAVDEDGYVPVICGLSRCNEKDIRTAWEAV 180

Query: 181 KYAKRPRIHTFIATSEIHMEHKLRKTREQVIDIARNMVRFAQSLGCDDVEFSPEDAGRSD 240
           KY+KRPRIHTFIATSEIHMEHKLRKTREQVI+IARNMV+FA+SLGCDDVEFSPEDAGRSD
Sbjct: 181 KYSKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVQFARSLGCDDVEFSPEDAGRSD 240

Query: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADLKANTPGIDNVIISTHCQNDL 300
           REFLYQILGEVIKAGATTLNIPDTVGYTMP EFG LIAD+K+NTPGI+NVIISTHCQNDL
Sbjct: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISTHCQNDL 300

Query: 301 GLATSNTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360
           GLAT+NTVAGACAGARQ+EVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH
Sbjct: 301 GLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360

Query: 361 IFLTSKMVEEYTGLNVQPRKAIVGANAFAHESGIHQDGMLKHK----------------- 420
           IFLTSKMVEEYTGLNVQP KAIVGANAFAHESGIHQDGMLKHK                 
Sbjct: 361 IFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIIAPEDIGYERSN 420

Query: 421 ------GKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD 448
                 GKLSGRHALKSRL+ELGYELDNE LDNVFWRFKAVAEQKKRVTD
Sbjct: 421 DAGIVLGKLSGRHALKSRLVELGYELDNEKLDNVFWRFKAVAEQKKRVTD 470

[Close]

BLAST of CmaCh01G003510 vs. ExPASy TrEMBL
Match: A0A6J1F3K1 (2-isopropylmalate synthase OS=Cucurbita moschata OX=3662 GN=LOC111441856 PE=3 SV=1)

HSP 1 Score: 785.0 bits (2026), Expect = 1.7e-223
Identity = 402/470 (85.53%), Postives = 419/470 (89.15%), Query Frame = 0


Query: 1   MAVPVTLSSPNHFFVRHSNSQFPSCHRSYNRNLQALKNSSWSLSFRTVADNVSRIYCSRT 60
           MA+P  LS PN F VR  NSQF + HR Y+RNLQALKN S SLSFRT  D+VSR+YC +T
Sbjct: 1   MAMPAALSRPNQFLVRQPNSQFSARHRIYSRNLQALKNPSRSLSFRTATDHVSRVYCCQT 60

Query: 61  ESVVSNSKSNSSIRRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKLDIARQLV 120
           E VV NS+S SSIRRP YIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKLDIARQL 
Sbjct: 61  ERVVLNSESKSSIRRPSYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKLDIARQLA 120

Query: 121 KLGVDIIEAGFPAASKEDFEAVKMIAAEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAV 180
           KLGVDIIEAGFPAASKEDFEAVKMIA EVGNAVD DGYVPVICGLSRCNE DIRTAWEAV
Sbjct: 121 KLGVDIIEAGFPAASKEDFEAVKMIAKEVGNAVDADGYVPVICGLSRCNEKDIRTAWEAV 180

Query: 181 KYAKRPRIHTFIATSEIHMEHKLRKTREQVIDIARNMVRFAQSLGCDDVEFSPEDAGRSD 240
           KY+KRPRIHTFIATSEIHMEHKLRKTREQVI+IARNMV+FA+SLGCDDVEFSPEDAGRSD
Sbjct: 181 KYSKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVQFARSLGCDDVEFSPEDAGRSD 240

Query: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADLKANTPGIDNVIISTHCQNDL 300
           REFLYQILGEVIKAGATTLNIPDTVGYTMP EFG LIAD+K+NTPGI+NVIISTHCQNDL
Sbjct: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISTHCQNDL 300

Query: 301 GLATSNTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360
           GLAT+NTVAGACAGARQ+EVTINGIGERAGNASLEEVVMALQCRGEHVLGGL TGINSRH
Sbjct: 301 GLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGINSRH 360

Query: 361 IFLTSKMVEEYTGLNVQPRKAIVGANAFAHESGIHQDGMLKHK----------------- 420
           IFLTSKMVEEYTGLNVQP KAIVGANAFAHESGIHQDGMLKHK                 
Sbjct: 361 IFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIIAPEDIGYERSN 420

Query: 421 ------GKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD 448
                 GKLSGRHALKSRL+ELGYELDNE LDNVFWRFKAVAEQKKRVTD
Sbjct: 421 DAGIVLGKLSGRHALKSRLVELGYELDNEKLDNVFWRFKAVAEQKKRVTD 470

[Close]

BLAST of CmaCh01G003510 vs. ExPASy TrEMBL
Match: A0A5D3BP53 (2-isopropylmalate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G00350 PE=3 SV=1)

HSP 1 Score: 771.5 bits (1991), Expect = 1.9e-219
Identity = 398/473 (84.14%), Postives = 413/473 (87.32%), Query Frame = 0


Query: 1   MAVPVTLSSPNHFFVRHSNSQFPSCHRSYNRNLQALKNSSWSLSFRTVA---DNVSRIYC 60
           MA+P  LS PNHF  R S+S FP+ H  Y RN Q LKN S SLSF+T A   DNVSRIYC
Sbjct: 1   MALPAALSRPNHFLARQSDSLFPARHPIYTRNFQVLKNPSRSLSFKTAAAAPDNVSRIYC 60

Query: 61  SRTESVVSNSKSNSSIRRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKLDIAR 120
             TESVVSNS   SS RRPPYIPNRIPD SYVRIFDTTLRDGEQSPGASLT KEKLDIAR
Sbjct: 61  CLTESVVSNSGPKSSTRRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTTKEKLDIAR 120

Query: 121 QLVKLGVDIIEAGFPAASKEDFEAVKMIAAEVGNAVDEDGYVPVICGLSRCNENDIRTAW 180
           QL KLGVDIIEAGFPAASKEDFEAVKMIA E+G+AVDEDGYVPVICGLSRCNE DIRTAW
Sbjct: 121 QLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGSAVDEDGYVPVICGLSRCNEKDIRTAW 180

Query: 181 EAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIDIARNMVRFAQSLGCDDVEFSPEDAG 240
           EAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVI+IARNMVRFA+SLGCDDVEFSPEDAG
Sbjct: 181 EAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDAG 240

Query: 241 RSDREFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADLKANTPGIDNVIISTHCQ 300
           RSDREFLYQILGEVIKAGATTLNIPDTVGYTMP EFG LIAD+K+NTPGI+NVIISTHCQ
Sbjct: 241 RSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISTHCQ 300

Query: 301 NDLGLATSNTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGIN 360
           NDLGLAT+NTVAGACAGARQ+EVTINGIGERAGNASLEEVVMALQCRGEHVLGGL TGIN
Sbjct: 301 NDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGIN 360

Query: 361 SRHIFLTSKMVEEYTGLNVQPRKAIVGANAFAHESGIHQDGMLKHK-------------- 420
            RHIFLTSKMVEE+TGLNVQP KAIVGANAFAH SGIHQDGMLKHK              
Sbjct: 361 PRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGYE 420

Query: 421 ---------GKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD 448
                    GKLSGRHALKSRLLELGYELD+ENL NVFWRFKAVAEQKKRVTD
Sbjct: 421 RSNDAGIVLGKLSGRHALKSRLLELGYELDSENLHNVFWRFKAVAEQKKRVTD 473

[Close]

BLAST of CmaCh01G003510 vs. ExPASy TrEMBL
Match: A0A1S3C4S6 (2-isopropylmalate synthase OS=Cucumis melo OX=3656 GN=LOC103496846 PE=3 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 4.2e-219
Identity = 398/476 (83.61%), Postives = 413/476 (86.76%), Query Frame = 0


Query: 1   MAVPVTLSSPNHFFVRHSNSQFPSCHRSYNRNLQALKNSSWSLSFRTVA------DNVSR 60
           MA+P  LS PNHF  R S+S FP+ H  Y RN Q LKN S SLSF+T A      DNVSR
Sbjct: 1   MALPAALSRPNHFLARQSDSLFPARHPIYTRNFQVLKNPSRSLSFKTAAAAAAAPDNVSR 60

Query: 61  IYCSRTESVVSNSKSNSSIRRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKLD 120
           IYC  TESVVSNS   SS RRPPYIPNRIPD SYVRIFDTTLRDGEQSPGASLT KEKLD
Sbjct: 61  IYCCLTESVVSNSGPKSSTRRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTTKEKLD 120

Query: 121 IARQLVKLGVDIIEAGFPAASKEDFEAVKMIAAEVGNAVDEDGYVPVICGLSRCNENDIR 180
           IARQL KLGVDIIEAGFPAASKEDFEAVKMIA E+G+AVDEDGYVPVICGLSRCNE DIR
Sbjct: 121 IARQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGSAVDEDGYVPVICGLSRCNEKDIR 180

Query: 181 TAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIDIARNMVRFAQSLGCDDVEFSPE 240
           TAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVI+IARNMVRFA+SLGCDDVEFSPE
Sbjct: 181 TAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPE 240

Query: 241 DAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADLKANTPGIDNVIIST 300
           DAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMP EFG LIAD+K+NTPGI+NVIIST
Sbjct: 241 DAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIIST 300

Query: 301 HCQNDLGLATSNTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRT 360
           HCQNDLGLAT+NTVAGACAGARQ+EVTINGIGERAGNASLEEVVMALQCRGEHVLGGL T
Sbjct: 301 HCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHT 360

Query: 361 GINSRHIFLTSKMVEEYTGLNVQPRKAIVGANAFAHESGIHQDGMLKHK----------- 420
           GIN RHIFLTSKMVEE+TGLNVQP KAIVGANAFAH SGIHQDGMLKHK           
Sbjct: 361 GINPRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDI 420

Query: 421 ------------GKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD 448
                       GKLSGRHALKSRLLELGYELD+ENL NVFWRFKAVAEQKKRVTD
Sbjct: 421 GYERSNDAGIVLGKLSGRHALKSRLLELGYELDSENLHNVFWRFKAVAEQKKRVTD 476

[Close]

BLAST of CmaCh01G003510 vs. NCBI nr
Match: XP_023523405.1 (2-isopropylmalate synthase 2, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 852.0 bits (2200), Expect = 2.3e-243
Identity = 436/470 (92.77%), Postives = 440/470 (93.62%), Query Frame = 0


Query: 1   MAVPVTLSSPNHFFVRHSNSQFPSCHRSYNRNLQALKNSSWSLSFRTVADNVSRIYCSRT 60
           MAVPVTLSSPNHF VRHSNSQFPSCHR YNRN QALKNSS SLSFRTVADNVSRIYCS T
Sbjct: 1   MAVPVTLSSPNHFLVRHSNSQFPSCHRIYNRNFQALKNSSRSLSFRTVADNVSRIYCSHT 60

Query: 61  ESVVSNSKSNSSIRRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKLDIARQLV 120
           ESVVSNSKSNSSIRRPPYIPNRIPDSSYVR+FDTTLRDGEQSPGASLTAKEKLDIARQLV
Sbjct: 61  ESVVSNSKSNSSIRRPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIARQLV 120

Query: 121 KLGVDIIEAGFPAASKEDFEAVKMIAAEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAV 180
           KLGVDIIEAGFPAASKEDFEAVKMIAAEVGNAVD DGYVPVICGLSRCNENDIRTAWEAV
Sbjct: 121 KLGVDIIEAGFPAASKEDFEAVKMIAAEVGNAVDSDGYVPVICGLSRCNENDIRTAWEAV 180

Query: 181 KYAKRPRIHTFIATSEIHMEHKLRKTREQVIDIARNMVRFAQSLGCDDVEFSPEDAGRSD 240
           KYAKRPRIHTFIATSEIHMEHKLRKTREQVIDIARNMVRFA+SLGCDDVEFSPEDAGRSD
Sbjct: 181 KYAKRPRIHTFIATSEIHMEHKLRKTREQVIDIARNMVRFARSLGCDDVEFSPEDAGRSD 240

Query: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADLKANTPGIDNVIISTHCQNDL 300
           REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIAD+KANTPGIDNVIISTHCQNDL
Sbjct: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHCQNDL 300

Query: 301 GLATSNTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360
           GLAT+NTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH
Sbjct: 301 GLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360

Query: 361 IFLTSKMVEEYTGLNVQPRKAIVGANAFAHESGIHQDGMLKHK----------------- 420
           IFLTSKMVEEYTGLNVQP KAIVGANAFAHESGIHQDGMLKHK                 
Sbjct: 361 IFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSN 420

Query: 421 ------GKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD 448
                 GKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD
Sbjct: 421 DAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD 470

[Close]

BLAST of CmaCh01G003510 vs. NCBI nr
Match: XP_022948501.1 (2-isopropylmalate synthase 2, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 851.3 bits (2198), Expect = 3.9e-243
Identity = 435/470 (92.55%), Postives = 442/470 (94.04%), Query Frame = 0


Query: 1   MAVPVTLSSPNHFFVRHSNSQFPSCHRSYNRNLQALKNSSWSLSFRTVADNVSRIYCSRT 60
           MAVPVTLSSPNHF VRHSNSQFPSCHR YNRNLQALKNSS SLSFRTVADNVSRIYCS T
Sbjct: 1   MAVPVTLSSPNHFLVRHSNSQFPSCHRRYNRNLQALKNSSRSLSFRTVADNVSRIYCSHT 60

Query: 61  ESVVSNSKSNSSIRRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKLDIARQLV 120
           ESVVSNSKSNSSI+RPPYIPNRIPDSSYVR+FDTTLRDGEQSPGASLTAKEKLDIARQLV
Sbjct: 61  ESVVSNSKSNSSIKRPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIARQLV 120

Query: 121 KLGVDIIEAGFPAASKEDFEAVKMIAAEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAV 180
           KLGVDIIEAGFPAASKEDFEAVKMIAAEVGNAV+EDGYVPVICGLSRCNENDIRTAWEAV
Sbjct: 121 KLGVDIIEAGFPAASKEDFEAVKMIAAEVGNAVNEDGYVPVICGLSRCNENDIRTAWEAV 180

Query: 181 KYAKRPRIHTFIATSEIHMEHKLRKTREQVIDIARNMVRFAQSLGCDDVEFSPEDAGRSD 240
           KYAKRPRIHTFIATSEIHMEHKLRKTREQVI+IARNMVRFA+SLGCDDVEFSPEDAGRSD
Sbjct: 181 KYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDAGRSD 240

Query: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADLKANTPGIDNVIISTHCQNDL 300
           REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIAD+KANTPGIDNVIISTHCQNDL
Sbjct: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHCQNDL 300

Query: 301 GLATSNTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360
           GLAT+NTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH
Sbjct: 301 GLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360

Query: 361 IFLTSKMVEEYTGLNVQPRKAIVGANAFAHESGIHQDGMLKHK----------------- 420
           IFLTSKMVEEYTGLNVQP KAIVGANAFAHESGIHQDGMLKHK                 
Sbjct: 361 IFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSN 420

Query: 421 ------GKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD 448
                 GKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD
Sbjct: 421 DAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD 470

[Close]

BLAST of CmaCh01G003510 vs. NCBI nr
Match: KAG6607000.1 (2-isopropylmalate synthase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 851.3 bits (2198), Expect = 3.9e-243
Identity = 435/470 (92.55%), Postives = 442/470 (94.04%), Query Frame = 0


Query: 1   MAVPVTLSSPNHFFVRHSNSQFPSCHRSYNRNLQALKNSSWSLSFRTVADNVSRIYCSRT 60
           MAVPVTLSSPNHF VRHSNSQFPSCHR YNRNLQALKNSS SLSFRTVADNVSRIYCS T
Sbjct: 1   MAVPVTLSSPNHFLVRHSNSQFPSCHRRYNRNLQALKNSSRSLSFRTVADNVSRIYCSHT 60

Query: 61  ESVVSNSKSNSSIRRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKLDIARQLV 120
           ESVVSNSKSNSSI+RPPYIPNRIPDSSYVR+FDTTLRDGEQSPGASLTAKEKLDIARQLV
Sbjct: 61  ESVVSNSKSNSSIKRPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIARQLV 120

Query: 121 KLGVDIIEAGFPAASKEDFEAVKMIAAEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAV 180
           KLGVDIIEAGFPAASKEDFEAVKMIAAEVGNAV+EDGYVPVICGLSRCNENDIRTAWEAV
Sbjct: 121 KLGVDIIEAGFPAASKEDFEAVKMIAAEVGNAVNEDGYVPVICGLSRCNENDIRTAWEAV 180

Query: 181 KYAKRPRIHTFIATSEIHMEHKLRKTREQVIDIARNMVRFAQSLGCDDVEFSPEDAGRSD 240
           KYAKRPRIHTFIATSEIHMEHKLRKTREQVI+IARNMVRFA+SLGCDDVEFSPEDAGRSD
Sbjct: 181 KYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDAGRSD 240

Query: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADLKANTPGIDNVIISTHCQNDL 300
           REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIAD+KANTPGIDNVIISTHCQNDL
Sbjct: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHCQNDL 300

Query: 301 GLATSNTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360
           GLAT+NTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH
Sbjct: 301 GLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360

Query: 361 IFLTSKMVEEYTGLNVQPRKAIVGANAFAHESGIHQDGMLKHK----------------- 420
           IFLTSKMVEEYTGLNVQP KAIVGANAFAHESGIHQDGMLKHK                 
Sbjct: 361 IFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSN 420

Query: 421 ------GKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD 448
                 GKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD
Sbjct: 421 DAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD 470

[Close]

BLAST of CmaCh01G003510 vs. NCBI nr
Match: KAG7036701.1 (2-isopropylmalate synthase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 850.1 bits (2195), Expect = 8.6e-243
Identity = 434/470 (92.34%), Postives = 442/470 (94.04%), Query Frame = 0


Query: 1   MAVPVTLSSPNHFFVRHSNSQFPSCHRSYNRNLQALKNSSWSLSFRTVADNVSRIYCSRT 60
           MAVPVTLSSPNHF VRHSNSQFPSCHR YNRNLQALKNSS SLSFRTVADNVSRIYCS T
Sbjct: 1   MAVPVTLSSPNHFLVRHSNSQFPSCHRRYNRNLQALKNSSRSLSFRTVADNVSRIYCSHT 60

Query: 61  ESVVSNSKSNSSIRRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKLDIARQLV 120
           ESVVSNSKSNSSI+RPPYIPNRIPDSSYVR+FDTTLRDGEQSPGASLTAKEKLDIARQLV
Sbjct: 61  ESVVSNSKSNSSIKRPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIARQLV 120

Query: 121 KLGVDIIEAGFPAASKEDFEAVKMIAAEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAV 180
           KLGVDIIEAGFPAASKEDFEAVKMIAA+VGNAV+EDGYVPVICGLSRCNENDIRTAWEAV
Sbjct: 121 KLGVDIIEAGFPAASKEDFEAVKMIAADVGNAVNEDGYVPVICGLSRCNENDIRTAWEAV 180

Query: 181 KYAKRPRIHTFIATSEIHMEHKLRKTREQVIDIARNMVRFAQSLGCDDVEFSPEDAGRSD 240
           KYAKRPRIHTFIATSEIHMEHKLRKTREQVI+IARNMVRFA+SLGCDDVEFSPEDAGRSD
Sbjct: 181 KYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDAGRSD 240

Query: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADLKANTPGIDNVIISTHCQNDL 300
           REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIAD+KANTPGIDNVIISTHCQNDL
Sbjct: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHCQNDL 300

Query: 301 GLATSNTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360
           GLAT+NTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH
Sbjct: 301 GLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360

Query: 361 IFLTSKMVEEYTGLNVQPRKAIVGANAFAHESGIHQDGMLKHK----------------- 420
           IFLTSKMVEEYTGLNVQP KAIVGANAFAHESGIHQDGMLKHK                 
Sbjct: 361 IFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSN 420

Query: 421 ------GKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD 448
                 GKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD
Sbjct: 421 DAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD 470

[Close]

BLAST of CmaCh01G003510 vs. NCBI nr
Match: XP_023522725.1 (2-isopropylmalate synthase 2, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 795.8 bits (2054), Expect = 1.9e-226
Identity = 407/470 (86.60%), Postives = 423/470 (90.00%), Query Frame = 0


Query: 1   MAVPVTLSSPNHFFVRHSNSQFPSCHRSYNRNLQALKNSSWSLSFRTVADNVSRIYCSRT 60
           MA+P  LS PN F VR  NSQF + HR Y+RNLQ LKN S SLSFRT AD+VSR+YC +T
Sbjct: 1   MAMPAALSRPNQFLVRQPNSQFSARHRIYSRNLQVLKNPSRSLSFRTAADHVSRVYCCQT 60

Query: 61  ESVVSNSKSNSSIRRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKLDIARQLV 120
           E VV NS+S SSIRRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEK+DIARQL 
Sbjct: 61  ERVVLNSESKSSIRRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKVDIARQLA 120

Query: 121 KLGVDIIEAGFPAASKEDFEAVKMIAAEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAV 180
           KLGVDIIEAGFPAASKEDFEAVKMIA EVGNAVDEDGYVPVICGLSRCNE DIRTAWEAV
Sbjct: 121 KLGVDIIEAGFPAASKEDFEAVKMIAKEVGNAVDEDGYVPVICGLSRCNEKDIRTAWEAV 180

Query: 181 KYAKRPRIHTFIATSEIHMEHKLRKTREQVIDIARNMVRFAQSLGCDDVEFSPEDAGRSD 240
           KY+KRPRIHTFIATSEIHMEHKLRKTREQVI+IARNMV+FA+SLGCDDVEFSPEDAGRSD
Sbjct: 181 KYSKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVQFARSLGCDDVEFSPEDAGRSD 240

Query: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADLKANTPGIDNVIISTHCQNDL 300
           REFLYQILGEVIKAGATTLNIPDTVGYTMP EFG LIAD+K+NTPGI+NVIISTHCQNDL
Sbjct: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISTHCQNDL 300

Query: 301 GLATSNTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360
           GLAT+NTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH
Sbjct: 301 GLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360

Query: 361 IFLTSKMVEEYTGLNVQPRKAIVGANAFAHESGIHQDGMLKHK----------------- 420
           IFLTSKMVEEYTGLNVQP KAIVGANAFAHESGIHQDGMLKHK                 
Sbjct: 361 IFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSN 420

Query: 421 ------GKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD 448
                 GKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD
Sbjct: 421 DAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD 470

[Close]

BLAST of CmaCh01G003510 vs. TAIR 10
Match: AT1G74040.1 (2-isopropylmalate synthase 1 )

HSP 1 Score: 622.5 bits (1604), Expect = 2.7e-178
Identity = 311/397 (78.34%), Postives = 348/397 (87.66%), Query Frame = 0


Query: 74  RRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKLDIARQLVKLGVDIIEAGFPA 133
           RRP YIPNRI D +YVRIFDTTLRDGEQSPGA+LT+KEKLDIARQL KLGVDIIEAGFPA
Sbjct: 72  RRPNYIPNRISDPNYVRIFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPA 131

Query: 134 ASKEDFEAVKMIAAEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTFIA 193
           ASK+DFEAVK IA  VGN VDE+GYVPVICGLSRCN+ DI TAWEAVKYAKRPRIHTFIA
Sbjct: 132 ASKDDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIETAWEAVKYAKRPRIHTFIA 191

Query: 194 TSEIHMEHKLRKTREQVIDIARNMVRFAQSLGCDDVEFSPEDAGRSDREFLYQILGEVIK 253
           TS+IH+++KL+K++E+VI+IARNMVRFA+SLGC+DVEFSPEDAGRS+RE+LY+ILGEVIK
Sbjct: 192 TSDIHLKYKLKKSKEEVIEIARNMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVIK 251

Query: 254 AGATTLNIPDTVGYTMPVEFGNLIADLKANTPGIDNVIISTHCQNDLGLATSNTVAGACA 313
           AGATTLNIPDTVG T+P EFG LIAD+KANTPGI NVIISTHCQNDLGL+T+NT++GA +
Sbjct: 252 AGATTLNIPDTVGITLPSEFGQLIADIKANTPGIQNVIISTHCQNDLGLSTANTLSGAHS 311

Query: 314 GARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRHIFLTSKMVEEYTG 373
           GARQ+EVTINGIGERAGNASLEEVVMA++CRG+HVLGGL TGI++RHI +TSKMVEEYTG
Sbjct: 312 GARQVEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTG 371

Query: 374 LNVQPRKAIVGANAFAHESGIHQDGMLKHK-----------------------GKLSGRH 433
           +  QP KAIVGANAFAHESGIHQDGMLKHK                       GKLSGRH
Sbjct: 372 MQTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIMSPEEIGLERSNDAGIVLGKLSGRH 431

Query: 434 ALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD 448
           ALK RL ELGY LD+  L N+FWRFKAVAEQKKRVTD
Sbjct: 432 ALKDRLNELGYVLDDGQLSNLFWRFKAVAEQKKRVTD 468

[Close]

BLAST of CmaCh01G003510 vs. TAIR 10
Match: AT1G18500.1 (methylthioalkylmalate synthase-like 4 )

HSP 1 Score: 619.0 bits (1595), Expect = 3.0e-177
Identity = 306/396 (77.27%), Postives = 345/396 (87.12%), Query Frame = 0


Query: 75  RPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKLDIARQLVKLGVDIIEAGFPAA 134
           RP YIPNRI D +YVR+FDTTLRDGEQSPGA+LT+KEKLDIARQL KLGVDIIEAGFPAA
Sbjct: 75  RPEYIPNRISDPNYVRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAA 134

Query: 135 SKEDFEAVKMIAAEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTFIAT 194
           SK+DFEAVK IA  VGN VDE+GYVPVICGLSRCN+ DI  AW+AVKYAKRPRIHTFIAT
Sbjct: 135 SKDDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIERAWDAVKYAKRPRIHTFIAT 194

Query: 195 SEIHMEHKLRKTREQVIDIARNMVRFAQSLGCDDVEFSPEDAGRSDREFLYQILGEVIKA 254
           S+IH+E+KL+KT+ +VI+IAR+MVRFA+SLGC+DVEFSPEDAGRS+RE+LY+ILGEVIKA
Sbjct: 195 SDIHLEYKLKKTKAEVIEIARSMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKA 254

Query: 255 GATTLNIPDTVGYTMPVEFGNLIADLKANTPGIDNVIISTHCQNDLGLATSNTVAGACAG 314
           GATTLNIPDTVG T+P EFG LI DLKANTPGI+NV+ISTHCQNDLGL+T+NT++GA AG
Sbjct: 255 GATTLNIPDTVGITLPSEFGQLITDLKANTPGIENVVISTHCQNDLGLSTANTLSGAHAG 314

Query: 315 ARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRHIFLTSKMVEEYTGL 374
           ARQ+EVTINGIGERAGNASLEEVVMA++CRG+HVLGGL TGI++RHI +TSKMVEEYTG+
Sbjct: 315 ARQMEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGM 374

Query: 375 NVQPRKAIVGANAFAHESGIHQDGMLKHK-----------------------GKLSGRHA 434
             QP KAIVGANAFAHESGIHQDGMLKHK                       GKLSGRHA
Sbjct: 375 QTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEEIGLERSNDAGIVLGKLSGRHA 434

Query: 435 LKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD 448
           LK RL ELGY+LD+E L  +FWRFK VAEQKKRVTD
Sbjct: 435 LKDRLTELGYQLDDEQLSTIFWRFKTVAEQKKRVTD 470

[Close]

BLAST of CmaCh01G003510 vs. TAIR 10
Match: AT5G23010.1 (methylthioalkylmalate synthase 1 )

HSP 1 Score: 485.3 bits (1248), Expect = 5.2e-137
Identity = 247/423 (58.39%), Postives = 315/423 (74.47%), Query Frame = 0


Query: 55  IYCSRTESVVSNSKSNSS------IRRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLT 114
           ++ S   SV  N+ ++S+       R P YIPN++PD +YVR+FDTTLRDGEQSPG SLT
Sbjct: 45  LFISCCSSVSKNAATSSTDLKPVVERWPEYIPNKLPDGNYVRVFDTTLRDGEQSPGGSLT 104

Query: 115 AKEKLDIARQLVKLGVDIIEAGFPAASKEDFEAVKMIAAEVGNAVDED-GYVPVICGLSR 174
             +KL+IARQL KL VDI+E GFP +S+E+ E +K IA  VGN VDE+ GYVPVIC ++R
Sbjct: 105 PPQKLEIARQLAKLRVDIMEVGFPGSSEEELETIKTIAKTVGNEVDEETGYVPVICAIAR 164

Query: 175 CNENDIRTAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIDIARNMVRFAQSLGCD 234
           C   DI   WEA+KYAKRPRI  F +TS+IHM++KL+KT+E+VI++A + +RFA+SLG +
Sbjct: 165 CKHRDIEATWEALKYAKRPRILVFTSTSDIHMKYKLKKTQEEVIEMAVSSIRFAKSLGFN 224

Query: 235 DVEFSPEDAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADLKANTPGI 294
           D++F  ED GRSD++FL +ILGE IKAG T + I DTVG  MP E+G L+  LKANTPGI
Sbjct: 225 DIQFGCEDGGRSDKDFLCKILGEAIKAGVTVVTIGDTVGINMPHEYGELVTYLKANTPGI 284

Query: 295 DNVIISTHCQNDLGLATSNTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEH 354
           D+V+++ HC NDLGLAT+N++AG  AGARQ+EVTINGIGER+GNASLEEVVMAL+CRG +
Sbjct: 285 DDVVVAVHCHNDLGLATANSIAGIRAGARQVEVTINGIGERSGNASLEEVVMALKCRGAY 344

Query: 355 VLGGLRTGINSRHIFLTSKMVEEYTGLNVQPRKAIVGANAFAHESGIHQDGMLKHK---- 414
           V+ G+ T I++R I  TSKMV+EYTGL VQ  K IVGAN F HESGIHQDG+LK++    
Sbjct: 345 VINGVYTKIDTRQIMATSKMVQEYTGLYVQAHKPIVGANCFVHESGIHQDGILKNRSTYE 404

Query: 415 -------------------GKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKR 448
                              GKLSGRHA+K RL ELGYELD+E L+ VF  F+ + + KKR
Sbjct: 405 ILSPEDIGIVKSQNSGLVLGKLSGRHAVKDRLKELGYELDDEKLNAVFSLFRDLTKNKKR 464

[Close]

BLAST of CmaCh01G003510 vs. TAIR 10
Match: AT5G23020.1 (2-isopropylmalate synthase 2 )

HSP 1 Score: 480.7 bits (1236), Expect = 1.3e-135
Identity = 233/398 (58.54%), Postives = 305/398 (76.63%), Query Frame = 0


Query: 74  RRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKLDIARQLVKLGVDIIEAGFPA 133
           RRP YIPN++P  +YVR+ DTTLRDGEQSPGA+LT  +KL+IARQL KL VDI+E GFP 
Sbjct: 70  RRPEYIPNKLPHKNYVRVLDTTLRDGEQSPGAALTPPQKLEIARQLAKLRVDIMEVGFPV 129

Query: 134 ASKEDFEAVKMIAAEVGNAVDED-GYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTFI 193
           +S+E+FEA+K IA  VGN VDE+ GYVPVICG++RC + DI   WEA+KYAKRPR+  F 
Sbjct: 130 SSEEEFEAIKTIAKTVGNEVDEETGYVPVICGIARCKKRDIEATWEALKYAKRPRVMLFT 189

Query: 194 ATSEIHMEHKLRKTREQVIDIARNMVRFAQSLGCDDVEFSPEDAGRSDREFLYQILGEVI 253
           +TSEIHM++KL+KT+E+VI++A N V++A+SLG  D++F  ED GR++++F+ +ILGE I
Sbjct: 190 STSEIHMKYKLKKTKEEVIEMAVNSVKYAKSLGFKDIQFGCEDGGRTEKDFICKILGESI 249

Query: 254 KAGATTLNIPDTVGYTMPVEFGNLIADLKANTPGIDNVIISTHCQNDLGLATSNTVAGAC 313
           KAGATT+   DTVG  MP EFG L+A +  NTPG D+++ + HC NDLG+AT+NT++G C
Sbjct: 250 KAGATTVGFADTVGINMPQEFGELVAYVIENTPGADDIVFAIHCHNDLGVATANTISGIC 309

Query: 314 AGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRHIFLTSKMVEEYT 373
           AGARQ+EVTINGIGER+GNA LEEVVMAL+CRGE ++ G+ T I+SR I  TSKMV+E+T
Sbjct: 310 AGARQVEVTINGIGERSGNAPLEEVVMALKCRGESLMDGVYTKIDSRQIMATSKMVQEHT 369

Query: 374 GLNVQPRKAIVGANAFAHESGIHQDGMLKHK-----------------------GKLSGR 433
           G+ VQP K IVG N F HESGIHQDG+LK++                       GKLSGR
Sbjct: 370 GMYVQPHKPIVGDNCFVHESGIHQDGILKNRSTYEILSPEDVGIVKSENSGIVLGKLSGR 429

Query: 434 HALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTD 448
           HA+K RL ELGYE+ +E  +++F R++ + + KKR+TD
Sbjct: 430 HAVKDRLKELGYEISDEKFNDIFSRYRELTKDKKRITD 467

The following BLAST results are available for this feature:

BLAST of CmaCh01G003510 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C550	3.8e-177	78.34	2-isopropylmalate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=I...	[more]
Q9LPR4	4.2e-176	77.27	2-isopropylmalate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=I...	[more]
O04973	4.4e-173	74.69	2-isopropylmalate synthase A OS=Solanum pennellii OX=28526 GN=IPMSA PE=2 SV=1	[more]
O04974	2.4e-163	71.75	2-isopropylmalate synthase B OS=Solanum pennellii OX=28526 GN=IPMSB PE=2 SV=1	[more]
Q39891	6.4e-156	69.44	Probable 2-isopropylmalate synthase OS=Glycine max OX=3847 GN=GMN56 PE=2 SV=1	[more]

BLAST of CmaCh01G003510 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G9D1	1.9e-243	92.55	2-isopropylmalate synthase OS=Cucurbita moschata OX=3662 GN=LOC111452159 PE=3 SV...	[more]
A0A6J1J7V5	1.6e-226	86.38	2-isopropylmalate synthase OS=Cucurbita maxima OX=3661 GN=LOC111482524 PE=3 SV=1	[more]
A0A6J1F3K1	1.7e-223	85.53	2-isopropylmalate synthase OS=Cucurbita moschata OX=3662 GN=LOC111441856 PE=3 SV...	[more]
A0A5D3BP53	1.9e-219	84.14	2-isopropylmalate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A1S3C4S6	4.2e-219	83.61	2-isopropylmalate synthase OS=Cucumis melo OX=3656 GN=LOC103496846 PE=3 SV=1	[more]

BLAST of CmaCh01G003510 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023523405.1	2.3e-243	92.77	2-isopropylmalate synthase 2, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
XP_022948501.1	3.9e-243	92.55	2-isopropylmalate synthase 2, chloroplastic-like [Cucurbita moschata]	[more]
KAG6607000.1	3.9e-243	92.55	2-isopropylmalate synthase 2, chloroplastic, partial [Cucurbita argyrosperma sub...	[more]
KAG7036701.1	8.6e-243	92.34	2-isopropylmalate synthase 2, chloroplastic, partial [Cucurbita argyrosperma sub...	[more]
XP_023522725.1	1.9e-226	86.60	2-isopropylmalate synthase 2, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmaCh01G003510 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G74040.1	2.7e-178	78.34	2-isopropylmalate synthase 1	[more]
AT1G18500.1	3.0e-177	77.27	methylthioalkylmalate synthase-like 4	[more]
AT5G23010.1	5.2e-137	58.39	methylthioalkylmalate synthase 1	[more]
AT5G23020.1	1.3e-135	58.54	2-isopropylmalate synthase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000891	Pyruvate carboxyltransferase	PFAM	PF00682	HMGL-like	coord: 89..368 e-value: 5.7E-93 score: 311.3
IPR000891	Pyruvate carboxyltransferase	PROSITE	PS50991	PYR_CT	coord: 89..362 score: 40.675144
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 75..422 e-value: 1.7E-124 score: 417.5
None	No IPR available	PANTHER	PTHR10277:SF74	2-ISOPROPYLMALATE SYNTHASE-RELATED	coord: 404..448 coord: 66..404
None	No IPR available	PANTHER	PTHR10277	HOMOCITRATE SYNTHASE-RELATED	coord: 404..448 coord: 66..404
None	No IPR available	CDD	cd07940	DRE_TIM_IPMS	coord: 91..370 e-value: 8.60927E-180 score: 501.208
None	No IPR available	SUPERFAMILY	51569	Aldolase	coord: 86..386
IPR002034	Alpha-isopropylmalate/homocitrate synthase, conserved site	PROSITE	PS00815	AIPM_HOMOCIT_SYNTH_1	coord: 96..112

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh01G003510.1	CmaCh01G003510.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009098	leucine biosynthetic process
biological_process	GO:0019752	carboxylic acid metabolic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0003852	2-isopropylmalate synthase activity
molecular_function	GO:0046912	acyltransferase, acyl groups converted into alkyl on transfer
molecular_function	GO:0003824	catalytic activity