CmaCh01G002560 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh01G002560
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptioneukaryotic initiation factor 4A-III
LocationCma_Chr01: 1186484 .. 1190363 (+)
RNA-Seq ExpressionCmaCh01G002560
SyntenyCmaCh01G002560
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GACGGTCAGGATTCGATCCCACTGTGGAAAATTGGATAGGATTTTATTAGTCCGTACACAAGACAATAACGTTTTCCTCTTTCTTCCTTGATCGTTCATCTGCCCTAATCTGTCGTTTCATCTCAATTTCTTTCCCTCACCGGCGTCGGGAATTATCGAGAAATCTCTCCGGGAAGTCGATTTCATAGATCTCGGTAACTACGCCAACGGACATGGCGGCTGCAGCTACAACAAGCGTTGTGCCGCCGAATCGGCGGCGAGCGATTACCGCGCCGGAAGATAAGTTGGAATTCGAGACGACGGAGGGGGTTGAGCCGATCTTGAGTTTCGATCAGATGGGTATTAAGGATGATCTTCTCCGTGGAATATATGCATATGGTTTTGAAAAGCCCTCGGCCATTCAGCAAAGAGCCGTGAGGCCTATTATCGATGGTCGAGATGTCATTGCTCAGGCCCAATCTGGAACTGGCAAGACCTCCATGATTGCCCTTACCGTTTGCCAGATGGTTGATACCTCCAGCAGAGAGTGAGTTCCGTTTACGATGGAATATTCAATGTTCCACTTTTCTCTCCCTCTTTCTTTCTCTCTATTCTGTTTATTGCGTGCTTAAAATCTATTGTCGTTGTTTAGTTACTTGGCTAAAAATTCCAACTTGAAAGACAAAGATTGTTCGGATGTCAGTTGGTTCTCTCCACAAGAAGTTATGGCCTCGAGTTGACATTTTGGAAATTTTGCAAGGAAGCCAAATTTAATAAAACCTCTAAAAAATAACGATACATTCGTTTTTTTTTTCTCTAAAAGTTCGGAACATCTGATATTAATGTAATACGATCTTTTAAACTTCAAATTCATATGGTAATTATGCATCTTAATTCCAATCAGGGTGCAGGCCTTGATCTTGTCCCCTACGAGAGAATTGGCTACTCAGACCGAGAAGGTTATATTGGCAATTGGCGACTACATTAATATACAAGCACACGCATGTATTGGAGGCAAAAGTGTGGGTGAAGATATTAGAAAGCTTGAGTTTGGAGTTCAGGTTGTGTCAGGAACTCCTGGAAGAGTCTGTGACATGATCAAGAGAAGAACATTGCGTACCAGAGCCATTAAGTTGTTAGTTCTGGTAAGTTTGGGTGCCCTTCAATTTGTTTCTCTTGTTACCAGTTTTCTACGTTTTCATTTCATTTTCTAATATTGACTCTTTCAGGATGAATCTGATGAGATGCTGAGCAGAGGGTTTAAAGACCAAATTTATGATGTATATCGATATCTCCCACCAGAGCTTCAGGTTCTTTCTCTCTCCTTCCCTTCCCCACATTGTTTGTATATGATTGTGCCCATGAGTGGCCAAGAGGATACGTAAATGAACTGCAGATCCGTCCTTGAGTTGGTTGCTGAACACGACTTTAACATGCTCAACGTGAAAAATAATACTTAGTATATGTGTTCTTCACAGAAAGATGAAACCTTTGTATGTACAATAGTATTTTTTCTGCAATAATGCATGTAGTCGATGAATTATTTTGTGAAGGAAGAAATAGCGGGATTGTTTTATCAGGGCTGCTTTATCGAGTAATAACAGGACTGTTTAATTGAATGGGTTTTTATAGTTTCTCTGAAGTCTTTGAAGTGCCAGATACTTGATCTCATCTAACTGTATTTTAATTTGTATTTTTGCTTGAATTTGTTTCCTTCATGTGCCAGTTTGTAAATGACGTGATATTACTAACATGTTCCTGTACGTATGGTTTTTATAAATGTTATTGTGTGTAACCTCACGCACTCTTTACACGTATGGTTATGGGCAACCAGTGCTTTTCACCTTTTTTATGTAGTTTTATACTTTCTGCCTGTCTCTAGAACCAGATCTGTTGGTTAATCCAATGAGTTTCCCCCCCTCAATTTTAATTGTAGGTTGTCTTGATTTCTGCTACTCTTCCTCATGAAATCTTGGAGATGACGAATAAGTTTATGACAGATCCTGTGAGGATCCTTGTGAAGCGTGATGAGTTAACTTTGGAGGTTTGTAATATTTTTATTCTAGATGAATAAGTGCCTTTTGGATATAAATCACACCCTGATGCATACTTTTAATTTGACAGGGTATTAAGCAATTCTTCGTTGCAGTTGAGAGGGAAGAGTGGAAATTTGATACCTTATGTGATCTTTATGACACCTTGACTATCACCCAAGCCGTAATTTTCTGTAACACCAAGCGTAAGGTAATGTGATTGACAATTTTGTTTTAGATCTCCTAAAATGATCTTCTCTAGATCTTGTCAGACTCACCCTGACGCAGCAAATGTTCTAATTTTGGCCCTTTTGTATCTGTTTTCCTTTTTGCATGATTTTGAATATGTCGTGATCTTCTGCCTCCAGGTTGAATGGCTGACTGAGAAGATGCGCAGCAATAACTTCACAGTTTCACATATGCATGGAGACATGCCTCAAAAGGAAAGAGATGCAATTATGGGGGAGTTTCGATCGGGAACTACCCGTGTCCTAATCACTACTGATGTTTGGGCCCGAGGGCTTGATGTTCAGCAGGCAAGTTGTTGATTCGTTATTTTATTGGTTTTTTTTGTCAATGCACCTCACAAGTTAAAGTAACTAAATTTCAACCTGGTAATGGTTCAAGTTCGAATGGATAACTGATTATATGATCTGATCAAGTAATACATTTGCTCCATATTTTAACTTCTTTTAATGTTGAGTTGAGTTGTCTCTATAATGTCGGTAGAATGTAATTAGAGATTATATCTACATAATGATTTCTGGGTATCTTGTGGATCATAAGCATCAGTTCAATGGTCTTGTAGTTAATGAACATACCGTTAGTTTCAGTTGACTACTTAGATATTTTGTGGACTATATGCATCAGTTATAAGTTATGAAGATTTCTAAGGTTTAGACATTTTGTGGAAAAATGACCATGAGGATCTTTCTCCATTCTTGGAGAATCACTGGTTAGTTCTTTTTCATTCTCTAGGAAGCATATTAGAAAAATATCTCTGCATGATTAGATATAGAGATAGAGAGAAGATTGGGTACTGAATTCGGTAAGGACAAAAAATATACTTTTTCCTATTTTATGTTAATGAATTGCTGACTGTGGCAAGTATCCTGTGAAATCTAGAAACTCTTATGTTTATTTGCTTCAACTTCTGTCAGGTATCACTAGTGATAAATTATGACCTTCCAAACAATCGAGAACTTTACATTCACAGGATAGGAAGGTCTGGTCGGTTTGGGCGCAAGGTATGATACTCTGTTCTGTATTTCAGTTGATTTCCTACTATGCACTTAAAGTGAACATAATGGAGTGATTAAACTAGAATGGAGAACTGGCTCTGCTTCTGAATGGCGTTTGATGTTCGCTTTATTTCTGTTAAAACTTTTAACGTGAATTTATGGCTTTAACGCAGGGTGTTGCTATCAACTTCGTGAAAAGTGATGACATTAAGATCCTGAGAGATATTGAACAGTACTACAGTACCCAGATTGATGAGATGCCCATGAACGTTGCTGATTTGATTTAGCACTGCTTGATAATATTGTGTGCTGTGTGGAAGATTTGTGTTAATATGCTCGCACATGAGGTTTTGTGTCCTTTTAAGTGCCTCTTTATTGATGATATTTAGCATAGGAAGAAGTTTTCACAGCAACCTTTTTCTGAGTGGCTTGGAGTGTTCATAAGTTGTATAATACCCCTGCGGTTGTTTTTCATTTTCTCAAGGGAAAGGAACTCAGATTATATCTTTGATTGACTTGAAATATATAATATATTCTGAGATTAGTTGTGTTACGATTCCTATGTGTACAGTTTCATCAGTTTCATCAATATTCTTGAGCTCACTTAGAACAGTAGAGAAGGTTGAG

mRNA sequence

GACGGTCAGGATTCGATCCCACTGTGGAAAATTGGATAGGATTTTATTAGTCCGTACACAAGACAATAACGTTTTCCTCTTTCTTCCTTGATCGTTCATCTGCCCTAATCTGTCGTTTCATCTCAATTTCTTTCCCTCACCGGCGTCGGGAATTATCGAGAAATCTCTCCGGGAAGTCGATTTCATAGATCTCGGTAACTACGCCAACGGACATGGCGGCTGCAGCTACAACAAGCGTTGTGCCGCCGAATCGGCGGCGAGCGATTACCGCGCCGGAAGATAAGTTGGAATTCGAGACGACGGAGGGGGTTGAGCCGATCTTGAGTTTCGATCAGATGGGTATTAAGGATGATCTTCTCCGTGGAATATATGCATATGGTTTTGAAAAGCCCTCGGCCATTCAGCAAAGAGCCGTGAGGCCTATTATCGATGGTCGAGATGTCATTGCTCAGGCCCAATCTGGAACTGGCAAGACCTCCATGATTGCCCTTACCGTTTGCCAGATGGTTGATACCTCCAGCAGAGAGGTGCAGGCCTTGATCTTGTCCCCTACGAGAGAATTGGCTACTCAGACCGAGAAGGTTATATTGGCAATTGGCGACTACATTAATATACAAGCACACGCATGTATTGGAGGCAAAAGTGTGGGTGAAGATATTAGAAAGCTTGAGTTTGGAGTTCAGGTTGTGTCAGGAACTCCTGGAAGAGTCTGTGACATGATCAAGAGAAGAACATTGCGTACCAGAGCCATTAAGTTGTTAGTTCTGGATGAATCTGATGAGATGCTGAGCAGAGGGTTTAAAGACCAAATTTATGATGTATATCGATATCTCCCACCAGAGCTTCAGGTTGTCTTGATTTCTGCTACTCTTCCTCATGAAATCTTGGAGATGACGAATAAGTTTATGACAGATCCTGTGAGGATCCTTGTGAAGCGTGATGAGTTAACTTTGGAGGGTATTAAGCAATTCTTCGTTGCAGTTGAGAGGGAAGAGTGGAAATTTGATACCTTATGTGATCTTTATGACACCTTGACTATCACCCAAGCCGTAATTTTCTGTAACACCAAGCGTAAGGTTGAATGGCTGACTGAGAAGATGCGCAGCAATAACTTCACAGTTTCACATATGCATGGAGACATGCCTCAAAAGGAAAGAGATGCAATTATGGGGGAGTTTCGATCGGGAACTACCCGTGTCCTAATCACTACTGATGTTTGGGCCCGAGGGCTTGATGTATCACTAGTGATAAATTATGACCTTCCAAACAATCGAGAACTTTACATTCACAGGATAGGAAGGTCTGGTCGGTTTGGGCGCAAGGGTGTTGCTATCAACTTCGTGAAAAGTGATGACATTAAGATCCTGAGAGATATTGAACAGTACTACAGTACCCAGATTGATGAGATGCCCATGAACGTTGCTGATTTGATTTAGCACTGCTTGATAATATTGTGTGCTGTGTGGAAGATTTGTGTTAATATGCTCGCACATGAGGTTTTGTGTCCTTTTAAGTGCCTCTTTATTGATGATATTTAGCATAGGAAGAAGTTTTCACAGCAACCTTTTTCTGAGTGGCTTGGAGTGTTCATAAGTTGTATAATACCCCTGCGGTTGTTTTTCATTTTCTCAAGGGAAAGGAACTCAGATTATATCTTTGATTGACTTGAAATATATAATATATTCTGAGATTAGTTGTGTTACGATTCCTATGTGTACAGTTTCATCAGTTTCATCAATATTCTTGAGCTCACTTAGAACAGTAGAGAAGGTTGAG

Coding sequence (CDS)

ATGGCGGCTGCAGCTACAACAAGCGTTGTGCCGCCGAATCGGCGGCGAGCGATTACCGCGCCGGAAGATAAGTTGGAATTCGAGACGACGGAGGGGGTTGAGCCGATCTTGAGTTTCGATCAGATGGGTATTAAGGATGATCTTCTCCGTGGAATATATGCATATGGTTTTGAAAAGCCCTCGGCCATTCAGCAAAGAGCCGTGAGGCCTATTATCGATGGTCGAGATGTCATTGCTCAGGCCCAATCTGGAACTGGCAAGACCTCCATGATTGCCCTTACCGTTTGCCAGATGGTTGATACCTCCAGCAGAGAGGTGCAGGCCTTGATCTTGTCCCCTACGAGAGAATTGGCTACTCAGACCGAGAAGGTTATATTGGCAATTGGCGACTACATTAATATACAAGCACACGCATGTATTGGAGGCAAAAGTGTGGGTGAAGATATTAGAAAGCTTGAGTTTGGAGTTCAGGTTGTGTCAGGAACTCCTGGAAGAGTCTGTGACATGATCAAGAGAAGAACATTGCGTACCAGAGCCATTAAGTTGTTAGTTCTGGATGAATCTGATGAGATGCTGAGCAGAGGGTTTAAAGACCAAATTTATGATGTATATCGATATCTCCCACCAGAGCTTCAGGTTGTCTTGATTTCTGCTACTCTTCCTCATGAAATCTTGGAGATGACGAATAAGTTTATGACAGATCCTGTGAGGATCCTTGTGAAGCGTGATGAGTTAACTTTGGAGGGTATTAAGCAATTCTTCGTTGCAGTTGAGAGGGAAGAGTGGAAATTTGATACCTTATGTGATCTTTATGACACCTTGACTATCACCCAAGCCGTAATTTTCTGTAACACCAAGCGTAAGGTTGAATGGCTGACTGAGAAGATGCGCAGCAATAACTTCACAGTTTCACATATGCATGGAGACATGCCTCAAAAGGAAAGAGATGCAATTATGGGGGAGTTTCGATCGGGAACTACCCGTGTCCTAATCACTACTGATGTTTGGGCCCGAGGGCTTGATGTATCACTAGTGATAAATTATGACCTTCCAAACAATCGAGAACTTTACATTCACAGGATAGGAAGGTCTGGTCGGTTTGGGCGCAAGGGTGTTGCTATCAACTTCGTGAAAAGTGATGACATTAAGATCCTGAGAGATATTGAACAGTACTACAGTACCCAGATTGATGAGATGCCCATGAACGTTGCTGATTTGATTTAG

Protein sequence

MAAAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKPSAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQTEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNNFTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Homology
BLAST of CmaCh01G002560 vs. ExPASy Swiss-Prot
Match: P41380 (Eukaryotic initiation factor 4A-3 OS=Nicotiana plumbaginifolia OX=4092 PE=2 SV=1)

HSP 1 Score: 707.2 bits (1824), Expect = 1.1e-202
Identity = 357/389 (91.77%), Postives = 370/389 (95.12%), Query Frame = 0

Query: 22  EDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKPSAIQQRAVRPIIDGRDVIAQA 81
           ED+L FET++GVEPI SF +MGIKDDLLRG+Y YGFEKPSAIQQRAV PII GRDVIAQA
Sbjct: 3   EDRLVFETSKGVEPIASFAEMGIKDDLLRGVYQYGFEKPSAIQQRAVLPIISGRDVIAQA 62

Query: 82  QSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQTEKVILAIGDYINIQAHACIG 141
           QSGTGKTSMIALTVCQ+VDT S EVQALILSPTRELA QTEKVILAIGDYIN+QAHACIG
Sbjct: 63  QSGTGKTSMIALTVCQIVDTKSSEVQALILSPTRELAAQTEKVILAIGDYINVQAHACIG 122

Query: 142 GKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLSRGFKDQIY 201
           GKSVGEDIRKLE GVQVVSGTPGRVCDMIKRRTLRTR IKLL+LDESDEMLSRGFKDQIY
Sbjct: 123 GKSVGEDIRKLEHGVQVVSGTPGRVCDMIKRRTLRTRGIKLLILDESDEMLSRGFKDQIY 182

Query: 202 DVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQFFVAVEREE 261
           DVYRYLPPELQVVLISATLP+EILE+T+KFMTDPVRILVKRDELTLEGIKQFFVAVE+EE
Sbjct: 183 DVYRYLPPELQVVLISATLPNEILEITSKFMTDPVRILVKRDELTLEGIKQFFVAVEKEE 242

Query: 262 WKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNNFTVSHMHGDMPQKERDAIMGE 321
           WKFDTLCDLYDTLTITQAVIFCNTKRKV+WLT KMR NNFTVS MHGDMPQKERDAIM E
Sbjct: 243 WKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTSKMRENNFTVSSMHGDMPQKERDAIMAE 302

Query: 322 FRSGTTRVLITTDVWARGLD---VSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVK 381
           FR GTTRVLITTDVWARGLD   VSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVK
Sbjct: 303 FRGGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVK 362

Query: 382 SDDIKILRDIEQYYSTQIDEMPMNVADLI 408
           SDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 363 SDDIKILRDIEQYYSTQIDEMPMNVADLI 391

BLAST of CmaCh01G002560 vs. ExPASy Swiss-Prot
Match: Q5VNM3 (Eukaryotic initiation factor 4A-III homolog A OS=Oryza sativa subsp. japonica OX=39947 GN=EIF4A3A PE=1 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 3.8e-200
Identity = 357/408 (87.50%), Postives = 377/408 (92.40%), Query Frame = 0

Query: 3   AAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKPSA 62
           AAATTS     RR      ++ L FET+ GVE I SFDQMGI+DDLLRGIYAYGFEKPSA
Sbjct: 2   AAATTS-----RRGPGAMDDENLTFETSPGVEVISSFDQMGIRDDLLRGIYAYGFEKPSA 61

Query: 63  IQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQTE 122
           IQQRAV PII GRDVIAQAQSGTGKTSMI+L+VCQ+VDT+ REVQALILSPTRELA QTE
Sbjct: 62  IQQRAVLPIISGRDVIAQAQSGTGKTSMISLSVCQIVDTAVREVQALILSPTRELAAQTE 121

Query: 123 KVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAIKL 182
           +V+LAIGD+INIQ HACIGGKS+GEDIRKLE GV VVSGTPGRVCDMIKRRTLRTRAIKL
Sbjct: 122 RVMLAIGDFINIQVHACIGGKSIGEDIRKLEHGVHVVSGTPGRVCDMIKRRTLRTRAIKL 181

Query: 183 LVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILVKR 242
           L+LDE+DEML RGFKDQIYDVYRYLPPELQV LISATLPHEILEMT+KFMTDPVRILVKR
Sbjct: 182 LILDEADEMLGRGFKDQIYDVYRYLPPELQVCLISATLPHEILEMTSKFMTDPVRILVKR 241

Query: 243 DELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNNFT 302
           DELTLEGIKQFFVAVE+EEWKFDTLCDLYDTLTITQAVIFCNTKRKV+WLTE+MRSNNFT
Sbjct: 242 DELTLEGIKQFFVAVEKEEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTERMRSNNFT 301

Query: 303 VSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLD---VSLVINYDLPNNRELYIH 362
           VS MHGDMPQKERDAIMGEFRSG TRVLITTDVWARGLD   VSLVINYDLPNNRELYIH
Sbjct: 302 VSAMHGDMPQKERDAIMGEFRSGATRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIH 361

Query: 363 RIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 408
           RIGRSGRFGRKGVAINFVK +DI+ILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 362 RIGRSGRFGRKGVAINFVKKEDIRILRDIEQYYSTQIDEMPMNVADLI 404

BLAST of CmaCh01G002560 vs. ExPASy Swiss-Prot
Match: Q10I26 (Eukaryotic initiation factor 4A-III homolog B OS=Oryza sativa subsp. japonica OX=39947 GN=EIF4A3B PE=1 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 3.8e-200
Identity = 357/408 (87.50%), Postives = 377/408 (92.40%), Query Frame = 0

Query: 3   AAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKPSA 62
           AAATTS     RR      ++ L FET+ GVE I SFDQMGI++DLLRGIYAYGFEKPSA
Sbjct: 2   AAATTS-----RRGPGAMDDENLTFETSPGVEVISSFDQMGIREDLLRGIYAYGFEKPSA 61

Query: 63  IQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQTE 122
           IQQRAV PII GRDVIAQAQSGTGKTSMI+L+VCQ+VDT+ REVQALILSPTRELA QTE
Sbjct: 62  IQQRAVLPIISGRDVIAQAQSGTGKTSMISLSVCQIVDTAVREVQALILSPTRELAAQTE 121

Query: 123 KVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAIKL 182
           +V+LAIGDYINIQ HACIGGKS+GEDIRKLE GV VVSGTPGRVCDMIKRRTLRTRAIKL
Sbjct: 122 RVMLAIGDYINIQVHACIGGKSIGEDIRKLEHGVHVVSGTPGRVCDMIKRRTLRTRAIKL 181

Query: 183 LVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILVKR 242
           L+LDE+DEML RGFKDQIYDVYRYLPPELQV LISATLPHEILEMT+KFMTDPVRILVKR
Sbjct: 182 LILDEADEMLGRGFKDQIYDVYRYLPPELQVCLISATLPHEILEMTSKFMTDPVRILVKR 241

Query: 243 DELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNNFT 302
           DELTLEGIKQFFVAVE+EEWKFDTLCDLYDTLTITQAVIFCNTKRKV+WLTE+MRSNNFT
Sbjct: 242 DELTLEGIKQFFVAVEKEEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTERMRSNNFT 301

Query: 303 VSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLD---VSLVINYDLPNNRELYIH 362
           VS MHGDMPQKERDAIMGEFRSG TRVLITTDVWARGLD   VSLVINYDLPNNRELYIH
Sbjct: 302 VSAMHGDMPQKERDAIMGEFRSGATRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIH 361

Query: 363 RIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 408
           RIGRSGRFGRKGVAINFVK +DI+ILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 362 RIGRSGRFGRKGVAINFVKKEDIRILRDIEQYYSTQIDEMPMNVADLI 404

BLAST of CmaCh01G002560 vs. ExPASy Swiss-Prot
Match: Q94A52 (Eukaryotic initiation factor 4A-III homolog OS=Arabidopsis thaliana OX=3702 GN=EIF4A3 PE=1 SV=2)

HSP 1 Score: 686.8 bits (1771), Expect = 1.5e-196
Identity = 346/397 (87.15%), Postives = 371/397 (93.45%), Query Frame = 0

Query: 14  RRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKPSAIQQRAVRPIID 73
           RR      +DKL FETT+G+EPI SF+ MGIK+D+LRG+Y YGFEKPSAIQQRAV PI+ 
Sbjct: 12  RRGGGAMDDDKLVFETTDGIEPITSFNDMGIKEDVLRGVYEYGFEKPSAIQQRAVMPILQ 71

Query: 74  GRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQTEKVILAIGDYIN 133
           GRDVIAQAQSGTGKTSMIAL+VCQ+VDTSSREVQALILSPTRELATQTEK I AIG + N
Sbjct: 72  GRDVIAQAQSGTGKTSMIALSVCQVVDTSSREVQALILSPTRELATQTEKTIQAIGLHAN 131

Query: 134 IQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLS 193
           IQAHACIGG SVGEDIRKLE GV VVSGTPGRVCDMIKRR+LRTRAIKLL+LDESDEMLS
Sbjct: 132 IQAHACIGGNSVGEDIRKLEHGVHVVSGTPGRVCDMIKRRSLRTRAIKLLILDESDEMLS 191

Query: 194 RGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQF 253
           RGFKDQIYDVYRYLPP+LQV L+SATLPHEILEMT+KFMT+PV+ILVKRDELTLEGIKQF
Sbjct: 192 RGFKDQIYDVYRYLPPDLQVCLVSATLPHEILEMTSKFMTEPVKILVKRDELTLEGIKQF 251

Query: 254 FVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNNFTVSHMHGDMPQK 313
           FVAVE+EEWKFDTLCDLYDTLTITQAVIFCNTKRKV++L+EKMRS+NFTVS MHGDMPQK
Sbjct: 252 FVAVEKEEWKFDTLCDLYDTLTITQAVIFCNTKRKVDYLSEKMRSHNFTVSSMHGDMPQK 311

Query: 314 ERDAIMGEFRSGTTRVLITTDVWARGLD---VSLVINYDLPNNRELYIHRIGRSGRFGRK 373
           ERDAIM EFRSG +RVLITTDVWARG+D   VSLVINYDLPNNRELYIHRIGRSGRFGRK
Sbjct: 312 ERDAIMNEFRSGDSRVLITTDVWARGIDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRK 371

Query: 374 GVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 408
           GVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 372 GVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 408

BLAST of CmaCh01G002560 vs. ExPASy Swiss-Prot
Match: B7ZTW1 (Eukaryotic initiation factor 4A-III OS=Xenopus tropicalis OX=8364 GN=eif4a3 PE=2 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 4.6e-182
Identity = 322/415 (77.59%), Postives = 367/415 (88.43%), Query Frame = 0

Query: 1   MAAAATTS---VVPPNRRRAITAPED--KLEFETTEGVEPILSFDQMGIKDDLLRGIYAY 60
           MAAAA T+   V   + R+ +   ED  K+EFET+E V+   +FD MG+++DLLRGIYAY
Sbjct: 1   MAAAAVTAVAGVTSGSSRKRLLREEDMTKVEFETSEEVDVTPTFDTMGLREDLLRGIYAY 60

Query: 61  GFEKPSAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTR 120
           GFEKPSAIQQRA++ II GRDVIAQ+QSGTGKT+   ++V Q +D   RE QALIL+PTR
Sbjct: 61  GFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFCVSVLQCLDIQVRETQALILAPTR 120

Query: 121 ELATQTEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTL 180
           ELA Q +KV+LA+GDY+N+Q HACIGG +VGEDIRKL++G  VV+GTPGRV DMI+RR+L
Sbjct: 121 ELAGQIQKVLLALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSL 180

Query: 181 RTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDP 240
           RTRAIK+LVLDE+DEML++GFK+QIYDVYRYLPP  QV LISATLPHEILEMTNKFMTDP
Sbjct: 181 RTRAIKMLVLDEADEMLNKGFKEQIYDVYRYLPPATQVCLISATLPHEILEMTNKFMTDP 240

Query: 241 VRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEK 300
           +RILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKV+WLTEK
Sbjct: 241 IRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEK 300

Query: 301 MRSNNFTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLD---VSLVINYDLPN 360
           MR  NFTVS MHGDMPQKER++IM EFRSG +RVLI+TDVWARGLD   VSL+INYDLPN
Sbjct: 301 MREANFTVSSMHGDMPQKERESIMKEFRSGASRVLISTDVWARGLDVPQVSLIINYDLPN 360

Query: 361 NRELYIHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 408
           NRELYIHRIGRSGR+GRKGVAINFVK+DDI+ILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 NRELYIHRIGRSGRYGRKGVAINFVKNDDIRILRDIEQYYSTQIDEMPMNVADLI 415

BLAST of CmaCh01G002560 vs. ExPASy TrEMBL
Match: A0A6J1K814 (eukaryotic initiation factor 4A-3-like OS=Cucurbita maxima OX=3661 GN=LOC111493076 PE=4 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 4.9e-227
Identity = 407/410 (99.27%), Postives = 407/410 (99.27%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120
           SAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLD---VSLVINYDLPNNRELY 360
           FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLD   VSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 408
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of CmaCh01G002560 vs. ExPASy TrEMBL
Match: A0A6J1GB02 (eukaryotic initiation factor 4A-3-like OS=Cucurbita moschata OX=3662 GN=LOC111452540 PE=4 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 4.9e-227
Identity = 407/410 (99.27%), Postives = 407/410 (99.27%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120
           SAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLD---VSLVINYDLPNNRELY 360
           FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLD   VSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 408
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of CmaCh01G002560 vs. ExPASy TrEMBL
Match: A0A6J1F3Q9 (eukaryotic initiation factor 4A-3 OS=Cucurbita moschata OX=3662 GN=LOC111442104 PE=4 SV=1)

HSP 1 Score: 791.6 bits (2043), Expect = 1.6e-225
Identity = 402/410 (98.05%), Postives = 407/410 (99.27%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRRA+TAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRAVTAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120
           SAIQQRAVRPII+GRDVIAQAQSGTGKTSMIALTVCQ+VDTSSREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQVVDTSSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAH+CIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHSCIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLL+LDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLILDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLD---VSLVINYDLPNNRELY 360
           FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLD   VSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 408
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of CmaCh01G002560 vs. ExPASy TrEMBL
Match: A0A5A7UAL9 (Eukaryotic initiation factor 4A-3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold43055G00150 PE=4 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 3.5e-225
Identity = 402/407 (98.77%), Postives = 406/407 (99.75%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRR I+APEDKLEFETTEGVEPIL+FDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRR-ISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120
           SAIQQRAVRPII+GRDVIAQAQSGTGKTSMIALTVCQMVDT+SREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVSLVINYDLPNNRELYIHR 360
           FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVSLVINYDLPNNRELYIHR
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVSLVINYDLPNNRELYIHR 360

Query: 361 IGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 408
           IGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 406

BLAST of CmaCh01G002560 vs. ExPASy TrEMBL
Match: A0A6J1J605 (eukaryotic initiation factor 4A-3 OS=Cucurbita maxima OX=3661 GN=LOC111482085 PE=4 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 4.6e-225
Identity = 401/410 (97.80%), Postives = 407/410 (99.27%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRRA++APEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRAVSAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120
           SAIQQRAVRPII+GRDVIAQAQSGTGKTSMIALTVCQ+VDTSSREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQVVDTSSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAH+CIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHSCIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLL+LDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLILDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLD---VSLVINYDLPNNRELY 360
           FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLD   VSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 408
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of CmaCh01G002560 vs. NCBI nr
Match: XP_022949081.1 (eukaryotic initiation factor 4A-3-like [Cucurbita moschata] >XP_022998447.1 eukaryotic initiation factor 4A-3-like [Cucurbita maxima] >XP_023525647.1 eukaryotic initiation factor 4A-3-like [Cucurbita pepo subsp. pepo] >KAG6606907.1 hypothetical protein SDJN03_00249, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 796.6 bits (2056), Expect = 1.0e-226
Identity = 407/410 (99.27%), Postives = 407/410 (99.27%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120
           SAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLD---VSLVINYDLPNNRELY 360
           FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLD   VSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 408
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of CmaCh01G002560 vs. NCBI nr
Match: XP_022935146.1 (eukaryotic initiation factor 4A-3 [Cucurbita moschata] >XP_023528747.1 eukaryotic initiation factor 4A-3 [Cucurbita pepo subsp. pepo] >KAG6581546.1 hypothetical protein SDJN03_21548, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 791.6 bits (2043), Expect = 3.3e-225
Identity = 402/410 (98.05%), Postives = 407/410 (99.27%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRRA+TAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRAVTAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120
           SAIQQRAVRPII+GRDVIAQAQSGTGKTSMIALTVCQ+VDTSSREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQVVDTSSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAH+CIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHSCIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLL+LDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLILDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLD---VSLVINYDLPNNRELY 360
           FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLD   VSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 408
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of CmaCh01G002560 vs. NCBI nr
Match: KAA0052762.1 (eukaryotic initiation factor 4A-3 [Cucumis melo var. makuwa])

HSP 1 Score: 790.4 bits (2040), Expect = 7.3e-225
Identity = 402/407 (98.77%), Postives = 406/407 (99.75%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRR I+APEDKLEFETTEGVEPIL+FDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRR-ISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120
           SAIQQRAVRPII+GRDVIAQAQSGTGKTSMIALTVCQMVDT+SREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVSLVINYDLPNNRELYIHR 360
           FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVSLVINYDLPNNRELYIHR
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVSLVINYDLPNNRELYIHR 360

Query: 361 IGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 408
           IGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 406

BLAST of CmaCh01G002560 vs. NCBI nr
Match: XP_022983495.1 (eukaryotic initiation factor 4A-3 [Cucurbita maxima] >KAG7018051.1 hypothetical protein SDJN02_19917, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 790.0 bits (2039), Expect = 9.5e-225
Identity = 401/410 (97.80%), Postives = 407/410 (99.27%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRRA++APEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRAVSAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120
           SAIQQRAVRPII+GRDVIAQAQSGTGKTSMIALTVCQ+VDTSSREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQVVDTSSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAH+CIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHSCIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLL+LDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLILDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLD---VSLVINYDLPNNRELY 360
           FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLD   VSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 408
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of CmaCh01G002560 vs. NCBI nr
Match: XP_004148574.1 (eukaryotic initiation factor 4A-3 [Cucumis sativus] >KGN58518.1 hypothetical protein Csa_001953 [Cucumis sativus])

HSP 1 Score: 789.6 bits (2038), Expect = 1.2e-224
Identity = 402/410 (98.05%), Postives = 406/410 (99.02%), Query Frame = 0

Query: 1   MAAAATTSVVPPNRRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKP 60
           MAAAATTSVVPPNRRR I+APEDKLEFETTEGVEPIL+FDQMGIKDDLLRGIYAYGFEKP
Sbjct: 1   MAAAATTSVVPPNRRRTISAPEDKLEFETTEGVEPILTFDQMGIKDDLLRGIYAYGFEKP 60

Query: 61  SAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQ 120
           SAIQQRAVRPII+GRDVIAQAQSGTGKTSMIALTVCQMVDT+SREVQALILSPTRELATQ
Sbjct: 61  SAIQQRAVRPIIEGRDVIAQAQSGTGKTSMIALTVCQMVDTTSREVQALILSPTRELATQ 120

Query: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180
           TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI
Sbjct: 121 TEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAI 180

Query: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240
           KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV
Sbjct: 181 KLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILV 240

Query: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300
           KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN
Sbjct: 241 KRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNN 300

Query: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLD---VSLVINYDLPNNRELY 360
           FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLD   VSLVINYDLPNNRELY
Sbjct: 301 FTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELY 360

Query: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 408
           IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 361 IHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 410

BLAST of CmaCh01G002560 vs. TAIR 10
Match: AT3G19760.1 (eukaryotic initiation factor 4A-III )

HSP 1 Score: 686.8 bits (1771), Expect = 1.1e-197
Identity = 346/397 (87.15%), Postives = 371/397 (93.45%), Query Frame = 0

Query: 14  RRRAITAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKPSAIQQRAVRPIID 73
           RR      +DKL FETT+G+EPI SF+ MGIK+D+LRG+Y YGFEKPSAIQQRAV PI+ 
Sbjct: 12  RRGGGAMDDDKLVFETTDGIEPITSFNDMGIKEDVLRGVYEYGFEKPSAIQQRAVMPILQ 71

Query: 74  GRDVIAQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQTEKVILAIGDYIN 133
           GRDVIAQAQSGTGKTSMIAL+VCQ+VDTSSREVQALILSPTRELATQTEK I AIG + N
Sbjct: 72  GRDVIAQAQSGTGKTSMIALSVCQVVDTSSREVQALILSPTRELATQTEKTIQAIGLHAN 131

Query: 134 IQAHACIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLS 193
           IQAHACIGG SVGEDIRKLE GV VVSGTPGRVCDMIKRR+LRTRAIKLL+LDESDEMLS
Sbjct: 132 IQAHACIGGNSVGEDIRKLEHGVHVVSGTPGRVCDMIKRRSLRTRAIKLLILDESDEMLS 191

Query: 194 RGFKDQIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQF 253
           RGFKDQIYDVYRYLPP+LQV L+SATLPHEILEMT+KFMT+PV+ILVKRDELTLEGIKQF
Sbjct: 192 RGFKDQIYDVYRYLPPDLQVCLVSATLPHEILEMTSKFMTEPVKILVKRDELTLEGIKQF 251

Query: 254 FVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNNFTVSHMHGDMPQK 313
           FVAVE+EEWKFDTLCDLYDTLTITQAVIFCNTKRKV++L+EKMRS+NFTVS MHGDMPQK
Sbjct: 252 FVAVEKEEWKFDTLCDLYDTLTITQAVIFCNTKRKVDYLSEKMRSHNFTVSSMHGDMPQK 311

Query: 314 ERDAIMGEFRSGTTRVLITTDVWARGLD---VSLVINYDLPNNRELYIHRIGRSGRFGRK 373
           ERDAIM EFRSG +RVLITTDVWARG+D   VSLVINYDLPNNRELYIHRIGRSGRFGRK
Sbjct: 312 ERDAIMNEFRSGDSRVLITTDVWARGIDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRK 371

Query: 374 GVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 408
           GVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI
Sbjct: 372 GVAINFVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 408

BLAST of CmaCh01G002560 vs. TAIR 10
Match: AT1G51380.1 (DEA(D/H)-box RNA helicase family protein )

HSP 1 Score: 570.1 bits (1468), Expect = 1.4e-162
Identity = 286/392 (72.96%), Postives = 337/392 (85.97%), Query Frame = 0

Query: 19  TAPEDKLEFETTEGVEPILSFDQMGIKDDLLRGIYAYGFEKPSAIQQRAVRPIIDGRDVI 78
           T  E+ L FETT+G++PI SFD MG+ D +LRG+Y YG++KPS IQQRA+ PI+ GRDVI
Sbjct: 4   TLDEENLVFETTKGIKPIKSFDDMGMNDKVLRGVYDYGYKKPSEIQQRALVPILKGRDVI 63

Query: 79  AQAQSGTGKTSMIALTVCQMVDTSSREVQALILSPTRELATQTEKVILAIGDYINIQAHA 138
           AQAQSGTGKTSMIA++VCQ+V+ SSR+VQ L+LSP+RELA+QTEK I AIG + NIQAHA
Sbjct: 64  AQAQSGTGKTSMIAISVCQIVNISSRKVQVLVLSPSRELASQTEKTIQAIGAHTNIQAHA 123

Query: 139 CIGGKSVGEDIRKLEFGVQVVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLSRGFKD 198
           CIGGKS+GEDI+KLE GV  VSGTPGRV DMIKR +L+T+A+KLLVLDESDEMLS+G KD
Sbjct: 124 CIGGKSIGEDIKKLERGVHAVSGTPGRVYDMIKRGSLQTKAVKLLVLDESDEMLSKGLKD 183

Query: 199 QIYDVYRYLPPELQVVLISATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQFFVAVE 258
           QIYDVYR LP ++QV LISATLP EILEMT KFMTDPVRILVK DELTLEGIKQ++V V+
Sbjct: 184 QIYDVYRALPHDIQVCLISATLPQEILEMTEKFMTDPVRILVKPDELTLEGIKQYYVDVD 243

Query: 259 REEWKFDTLCDLYDTLTITQAVIFCNTKRKVEWLTEKMRSNNFTVSHMHGDMPQKERDAI 318
           +EEWKFDTLCDLY  LTI QA+IFCNT++KV+WLTEKMRS+NF VS MHGD  QKERD I
Sbjct: 244 KEEWKFDTLCDLYGRLTINQAIIFCNTRQKVDWLTEKMRSSNFIVSSMHGDKRQKERDDI 303

Query: 319 MGEFRSGTTRVLITTDVWARGLD---VSLVINYDLPNNRELYIHRIGRSGRFGRKGVAIN 378
           M +FRS  +RVLI +DVWARG+D   VS VINYD+PNN ELYIHRIGR+GRFGR+GVAIN
Sbjct: 304 MNQFRSFKSRVLIASDVWARGIDVQTVSHVINYDIPNNPELYIHRIGRAGRFGREGVAIN 363

Query: 379 FVKSDDIKILRDIEQYYSTQIDEMPMNVADLI 408
           FVKS D+K L+DIE++Y T+I EMP   ADL+
Sbjct: 364 FVKSSDMKDLKDIERHYGTKIREMP---ADLV 392

BLAST of CmaCh01G002560 vs. TAIR 10
Match: AT3G13920.1 (eukaryotic translation initiation factor 4A1 )

HSP 1 Score: 493.0 bits (1268), Expect = 2.2e-139
Identity = 246/373 (65.95%), Postives = 302/373 (80.97%), Query Frame = 0

Query: 38  SFDQMGIKDDLLRGIYAYGFEKPSAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQ 97
           SFD MG++++LLRGIYAYGFEKPSAIQQR + P   G DVI QAQSGTGKT+     V Q
Sbjct: 40  SFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGVLQ 99

Query: 98  MVDTSSREVQALILSPTRELATQTEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQ 157
            +D S  + QAL+L+PTRELA Q EKV+ A+GDY+ ++ HAC+GG SV ED R L+ GV 
Sbjct: 100 QLDFSLIQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVH 159

Query: 158 VVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLIS 217
           VV GTPGRV DM+KR++LR   IK+ VLDE+DEMLSRGFKDQIYD+++ LPP++QV + S
Sbjct: 160 VVVGTPGRVFDMLKRQSLRADNIKMFVLDEADEMLSRGFKDQIYDIFQLLPPKIQVGVFS 219

Query: 218 ATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTIT 277
           AT+P E LE+T KFM+ PVRILVKRDELTLEGIKQF+V VE+EEWK +TLCDLY+TL IT
Sbjct: 220 ATMPPEALEITRKFMSKPVRILVKRDELTLEGIKQFYVNVEKEEWKLETLCDLYETLAIT 279

Query: 278 QAVIFCNTKRKVEWLTEKMRSNNFTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWA 337
           Q+VIF NT+RKV+WLT+KMRS + TVS  HGDM Q  RD IM EFRSG++RVLITTD+ A
Sbjct: 280 QSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLA 339

Query: 338 RGLD---VSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYST 397
           RG+D   VSLVIN+DLP   E Y+HRIGRSGRFGRKGVAINFV  DD ++L DI+++Y+ 
Sbjct: 340 RGIDVQQVSLVINFDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNV 399

Query: 398 QIDEMPMNVADLI 408
            ++E+P NVADL+
Sbjct: 400 VVEELPSNVADLL 412

BLAST of CmaCh01G002560 vs. TAIR 10
Match: AT3G13920.3 (eukaryotic translation initiation factor 4A1 )

HSP 1 Score: 493.0 bits (1268), Expect = 2.2e-139
Identity = 246/373 (65.95%), Postives = 302/373 (80.97%), Query Frame = 0

Query: 38  SFDQMGIKDDLLRGIYAYGFEKPSAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQ 97
           SFD MG++++LLRGIYAYGFEKPSAIQQR + P   G DVI QAQSGTGKT+     V Q
Sbjct: 30  SFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGVLQ 89

Query: 98  MVDTSSREVQALILSPTRELATQTEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQ 157
            +D S  + QAL+L+PTRELA Q EKV+ A+GDY+ ++ HAC+GG SV ED R L+ GV 
Sbjct: 90  QLDFSLIQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVH 149

Query: 158 VVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLIS 217
           VV GTPGRV DM+KR++LR   IK+ VLDE+DEMLSRGFKDQIYD+++ LPP++QV + S
Sbjct: 150 VVVGTPGRVFDMLKRQSLRADNIKMFVLDEADEMLSRGFKDQIYDIFQLLPPKIQVGVFS 209

Query: 218 ATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTIT 277
           AT+P E LE+T KFM+ PVRILVKRDELTLEGIKQF+V VE+EEWK +TLCDLY+TL IT
Sbjct: 210 ATMPPEALEITRKFMSKPVRILVKRDELTLEGIKQFYVNVEKEEWKLETLCDLYETLAIT 269

Query: 278 QAVIFCNTKRKVEWLTEKMRSNNFTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWA 337
           Q+VIF NT+RKV+WLT+KMRS + TVS  HGDM Q  RD IM EFRSG++RVLITTD+ A
Sbjct: 270 QSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLA 329

Query: 338 RGLD---VSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYST 397
           RG+D   VSLVIN+DLP   E Y+HRIGRSGRFGRKGVAINFV  DD ++L DI+++Y+ 
Sbjct: 330 RGIDVQQVSLVINFDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNV 389

Query: 398 QIDEMPMNVADLI 408
            ++E+P NVADL+
Sbjct: 390 VVEELPSNVADLL 402

BLAST of CmaCh01G002560 vs. TAIR 10
Match: AT1G54270.1 (eif4a-2 )

HSP 1 Score: 488.0 bits (1255), Expect = 7.2e-138
Identity = 243/373 (65.15%), Postives = 301/373 (80.70%), Query Frame = 0

Query: 38  SFDQMGIKDDLLRGIYAYGFEKPSAIQQRAVRPIIDGRDVIAQAQSGTGKTSMIALTVCQ 97
           SFD MG++++LLRGIYAYGFEKPSAIQQR + P   G DVI QAQSGTGKT+     V Q
Sbjct: 40  SFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGVLQ 99

Query: 98  MVDTSSREVQALILSPTRELATQTEKVILAIGDYINIQAHACIGGKSVGEDIRKLEFGVQ 157
            +D +  + QAL+L+PTRELA Q EKV+ A+GDY  ++ HAC+GG SV ED R L+ GV 
Sbjct: 100 QLDYALLQCQALVLAPTRELAQQIEKVMRALGDYQGVKVHACVGGTSVREDQRILQAGVH 159

Query: 158 VVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPELQVVLIS 217
           VV GTPGRV DM++R++LR   IK+ VLDE+DEMLSRGFKDQIYD+++ LPP++QV + S
Sbjct: 160 VVVGTPGRVFDMLRRQSLRPDCIKMFVLDEADEMLSRGFKDQIYDIFQLLPPKIQVGVFS 219

Query: 218 ATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTIT 277
           AT+P E LE+T KFM+ PVRILVKRDELTLEGIKQF+V VE+E+WK +TLCDLY+TL IT
Sbjct: 220 ATMPPEALEITRKFMSKPVRILVKRDELTLEGIKQFYVNVEKEDWKLETLCDLYETLAIT 279

Query: 278 QAVIFCNTKRKVEWLTEKMRSNNFTVSHMHGDMPQKERDAIMGEFRSGTTRVLITTDVWA 337
           Q+VIF NT+RKV+WLT+KMRS + TVS  HGDM Q  RD IM EFRSG++RVLITTD+ A
Sbjct: 280 QSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLA 339

Query: 338 RGLD---VSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVKSDDIKILRDIEQYYST 397
           RG+D   VSLVIN+DLP   E Y+HRIGRSGRFGRKGVAINFV  DD ++L DI+++Y+ 
Sbjct: 340 RGIDVQQVSLVINFDLPTQPENYLHRIGRSGRFGRKGVAINFVTLDDQRMLFDIQKFYNV 399

Query: 398 QIDEMPMNVADLI 408
            ++E+P NVADL+
Sbjct: 400 VVEELPSNVADLL 412

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P413801.1e-20291.77Eukaryotic initiation factor 4A-3 OS=Nicotiana plumbaginifolia OX=4092 PE=2 SV=1[more]
Q5VNM33.8e-20087.50Eukaryotic initiation factor 4A-III homolog A OS=Oryza sativa subsp. japonica OX... [more]
Q10I263.8e-20087.50Eukaryotic initiation factor 4A-III homolog B OS=Oryza sativa subsp. japonica OX... [more]
Q94A521.5e-19687.15Eukaryotic initiation factor 4A-III homolog OS=Arabidopsis thaliana OX=3702 GN=E... [more]
B7ZTW14.6e-18277.59Eukaryotic initiation factor 4A-III OS=Xenopus tropicalis OX=8364 GN=eif4a3 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1K8144.9e-22799.27eukaryotic initiation factor 4A-3-like OS=Cucurbita maxima OX=3661 GN=LOC1114930... [more]
A0A6J1GB024.9e-22799.27eukaryotic initiation factor 4A-3-like OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1F3Q91.6e-22598.05eukaryotic initiation factor 4A-3 OS=Cucurbita moschata OX=3662 GN=LOC111442104 ... [more]
A0A5A7UAL93.5e-22598.77Eukaryotic initiation factor 4A-3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A6J1J6054.6e-22597.80eukaryotic initiation factor 4A-3 OS=Cucurbita maxima OX=3661 GN=LOC111482085 PE... [more]
Match NameE-valueIdentityDescription
XP_022949081.11.0e-22699.27eukaryotic initiation factor 4A-3-like [Cucurbita moschata] >XP_022998447.1 euka... [more]
XP_022935146.13.3e-22598.05eukaryotic initiation factor 4A-3 [Cucurbita moschata] >XP_023528747.1 eukaryoti... [more]
KAA0052762.17.3e-22598.77eukaryotic initiation factor 4A-3 [Cucumis melo var. makuwa][more]
XP_022983495.19.5e-22597.80eukaryotic initiation factor 4A-3 [Cucurbita maxima] >KAG7018051.1 hypothetical ... [more]
XP_004148574.11.2e-22498.05eukaryotic initiation factor 4A-3 [Cucumis sativus] >KGN58518.1 hypothetical pro... [more]
Match NameE-valueIdentityDescription
AT3G19760.11.1e-19787.15eukaryotic initiation factor 4A-III [more]
AT1G51380.11.4e-16272.96DEA(D/H)-box RNA helicase family protein [more]
AT3G13920.12.2e-13965.95eukaryotic translation initiation factor 4A1 [more]
AT3G13920.32.2e-13965.95eukaryotic translation initiation factor 4A1 [more]
AT1G54270.17.2e-13865.15eif4a-2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014001Helicase superfamily 1/2, ATP-binding domainSMARTSM00487ultradead3coord: 56..253
e-value: 9.4E-52
score: 187.9
IPR014001Helicase superfamily 1/2, ATP-binding domainPROSITEPS51192HELICASE_ATP_BIND_1coord: 68..238
score: 25.31591
IPR001650Helicase, C-terminalSMARTSM00490helicmild6coord: 290..368
e-value: 1.2E-29
score: 114.5
IPR001650Helicase, C-terminalPFAMPF00271Helicase_Ccoord: 263..368
e-value: 1.7E-27
score: 95.9
IPR001650Helicase, C-terminalPROSITEPS51194HELICASE_CTERcoord: 249..407
score: 22.489365
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 27..245
e-value: 5.5E-64
score: 217.3
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 246..407
e-value: 3.1E-56
score: 191.2
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 105..390
IPR011545DEAD/DEAH box helicase domainPFAMPF00270DEADcoord: 62..224
e-value: 6.4E-41
score: 140.0
NoneNo IPR availablePANTHERPTHR47958ATP-DEPENDENT RNA HELICASE DBP3coord: 22..400
NoneNo IPR availablePANTHERPTHR47958:SF55BNAC03G41130D PROTEINcoord: 22..400
NoneNo IPR availableCDDcd18787SF2_C_DEADcoord: 250..377
e-value: 1.31259E-59
score: 188.1
NoneNo IPR availableCDDcd18045DEADc_EIF4AIII_DDX48coord: 39..239
e-value: 1.29384E-122
score: 351.77
IPR014014RNA helicase, DEAD-box type, Q motifPROSITEPS51195Q_MOTIFcoord: 37..65
score: 10.86482

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G002560.1CmaCh01G002560.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006413 translational initiation
cellular_component GO:0071013 catalytic step 2 spliceosome
cellular_component GO:0005730 nucleolus
molecular_function GO:0005524 ATP binding
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003729 mRNA binding
molecular_function GO:0003724 RNA helicase activity
molecular_function GO:0003743 translation initiation factor activity
molecular_function GO:0003676 nucleic acid binding