CmaCh01G001480 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh01G001480
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionpalmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like
LocationCma_Chr01: 658657 .. 660202 (-)
RNA-Seq ExpressionCmaCh01G001480
SyntenyCmaCh01G001480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTCTTGCTCCTCAAATGGAGGCAGCAACGAAGGAGGTGGAAGATAAAAATACCGATGAGAAGGCTATGGCCGTGAATGACAAAGCTCCAGTGAGGCCCCGTGGAAGGCCCTTTTTTCAAAGAAGATGGACAACTTTGGACAAACGTTCTGCTGGTCTTCTTTTGGTCTTGCATTTGCTTTGTCTCTTGGCGCCATTTCACTTCACTTGGTCAGCCTTCTGCCTCGCCTTTCTACTCTACAATTTCACGGGCCTCTTCGGTTTGACGCTTTCGTACCACCGACATCTCTCGCACAAGAGTTTCAAGCTTCCCAAGTGGCTTGAATACACGTTCGCTTATATTGGAGTCCTTACTCTTCAGGTTAATCATGTGTTATTTACCTTTCTAAATTTTTATTCTCATTTTTTTTTTCTTTTTTCATTAAGTATTACCTTTGTTATTATAGGGCGATCCAATTGATTGGGTGAGTACACACAGATATCATCATCAATTTGTCGATACAGACAGAGATCCACACAGTCCAAAACAAGGATTTTGGTTTGGTCATATTGCATGGATATTTGATTCGTATGGTTTGACTAAGAAAGTTTGTCCGAAACACGTTGAAGATTTTGAGGAAAGGAATCGAAAACGAAAGCGTTTTAGAAATATTTTTGTGGGATGTTTGAAATATGGAAGACCAAACAACGTGGAAGATTTACAAAAGCAGGCCTTTTATAGGTTTCTTCGTACAACAAACATTCTTCATCATTTTGGTCTTGCAATTGTCCTCAATGCCGTCGGAGGGCTTCCCTTCCTCGTATGGGGAATGGTAAGATTTTTCCTCGTACAATATCATACTACTTTTAATTTAAGGAAAGTGTTCGTAGGTTCATGTAATCAAGACTCAAATGGAACCTTTGTAGTAAGTGTAGTGTAACCGTTTAAGTATTCCGATAGTAGATATTAACTTTTTTGAACTCTCCAATCTATGTGGAATCTCACCATCTTCTTCCATTCGGGGTTCAGCGTCCTTGCTGGCACACTGCCATTGCATTAGTTAAGAAGGCTTTCTCTAAAAGTTTTTAAAATGCATAAGCTAAGAAGATTTTTCCACACTAAGTGAACATAATTTGATGGTTTGAATTGATCGAATAAATGCAGAGTGTAAGAACGGTATGCTTTCAACATGTCACATCTCCCTAAACTCTATCGGCCACATGTGGGGAAACCAGCAATGGAACACCGGGGATCAGTCAAGAAACAACTGGTTCGTCGAAGTTTTTTTTCGTTTTTACATTACATTTTCTTTTGTTGTCGTTGAGTAAATGAGCTAATGAAACGATTGGTTTTTAGGGTATTGGCATTGCTTATATTTGGGGAAGGGTGGCACAACCACCACCATGCATTTGAGTATTCAGCTCGACATGGCCTAGAATGGTGGCAGCTCGATGTAACCTGGTGGGTGATTTTGTTGCTTCAAGCTCTTGGGTTAGCAACCGATTTGAAATTACCCTCTCAACATCACATGCAGAAGTTGGTGTTTCAACCTAAATGCGAGTGA

mRNA sequence

ATGTCTCTTGCTCCTCAAATGGAGGCAGCAACGAAGGAGGTGGAAGATAAAAATACCGATGAGAAGGCTATGGCCGTGAATGACAAAGCTCCAGTGAGGCCCCGTGGAAGGCCCTTTTTTCAAAGAAGATGGACAACTTTGGACAAACGTTCTGCTGGTCTTCTTTTGGTCTTGCATTTGCTTTGTCTCTTGGCGCCATTTCACTTCACTTGGTCAGCCTTCTGCCTCGCCTTTCTACTCTACAATTTCACGGGCCTCTTCGGTTTGACGCTTTCGTACCACCGACATCTCTCGCACAAGAGTTTCAAGCTTCCCAAGTGGCTTGAATACACGTTCGCTTATATTGGAGTCCTTACTCTTCAGGGCGATCCAATTGATTGGGTGAGTACACACAGATATCATCATCAATTTGTCGATACAGACAGAGATCCACACAGTCCAAAACAAGGATTTTGGTTTGGTCATATTGCATGGATATTTGATTCGTATGGTTTGACTAAGAAAGTTTGTCCGAAACACGTTGAAGATTTTGAGGAAAGGAATCGAAAACGAAAGCGTTTTAGAAATATTTTTGTGGGATGTTTGAAATATGGAAGACCAAACAACGTGGAAGATTTACAAAAGCAGGCCTTTTATAGGTTTCTTCGTACAACAAACATTCTTCATCATTTTGGTCTTGCAATTGTCCTCAATGCCGTCGGAGGGCTTCCCTTCCTCGTATGGGGAATGCGTCCTTGCTGGCACACTGCCATTGCATTAGTTAAGAAGGCTTTCTCTAAAAAGTGTAAGAACGGTATGCTTTCAACATGTCACATCTCCCTAAACTCTATCGGCCACATGTGGGGAAACCAGCAATGGAACACCGGGGATCAGTCAAGAAACAACTGGGTATTGGCATTGCTTATATTTGGGGAAGGGTGGCACAACCACCACCATGCATTTGAGTATTCAGCTCGACATGGCCTAGAATGGTGGCAGCTCGATGTAACCTGGTGGGTGATTTTGTTGCTTCAAGCTCTTGGGTTAGCAACCGATTTGAAATTACCCTCTCAACATCACATGCAGAAGTTGGTGTTTCAACCTAAATGCGAGTGA

Coding sequence (CDS)

ATGTCTCTTGCTCCTCAAATGGAGGCAGCAACGAAGGAGGTGGAAGATAAAAATACCGATGAGAAGGCTATGGCCGTGAATGACAAAGCTCCAGTGAGGCCCCGTGGAAGGCCCTTTTTTCAAAGAAGATGGACAACTTTGGACAAACGTTCTGCTGGTCTTCTTTTGGTCTTGCATTTGCTTTGTCTCTTGGCGCCATTTCACTTCACTTGGTCAGCCTTCTGCCTCGCCTTTCTACTCTACAATTTCACGGGCCTCTTCGGTTTGACGCTTTCGTACCACCGACATCTCTCGCACAAGAGTTTCAAGCTTCCCAAGTGGCTTGAATACACGTTCGCTTATATTGGAGTCCTTACTCTTCAGGGCGATCCAATTGATTGGGTGAGTACACACAGATATCATCATCAATTTGTCGATACAGACAGAGATCCACACAGTCCAAAACAAGGATTTTGGTTTGGTCATATTGCATGGATATTTGATTCGTATGGTTTGACTAAGAAAGTTTGTCCGAAACACGTTGAAGATTTTGAGGAAAGGAATCGAAAACGAAAGCGTTTTAGAAATATTTTTGTGGGATGTTTGAAATATGGAAGACCAAACAACGTGGAAGATTTACAAAAGCAGGCCTTTTATAGGTTTCTTCGTACAACAAACATTCTTCATCATTTTGGTCTTGCAATTGTCCTCAATGCCGTCGGAGGGCTTCCCTTCCTCGTATGGGGAATGCGTCCTTGCTGGCACACTGCCATTGCATTAGTTAAGAAGGCTTTCTCTAAAAAGTGTAAGAACGGTATGCTTTCAACATGTCACATCTCCCTAAACTCTATCGGCCACATGTGGGGAAACCAGCAATGGAACACCGGGGATCAGTCAAGAAACAACTGGGTATTGGCATTGCTTATATTTGGGGAAGGGTGGCACAACCACCACCATGCATTTGAGTATTCAGCTCGACATGGCCTAGAATGGTGGCAGCTCGATGTAACCTGGTGGGTGATTTTGTTGCTTCAAGCTCTTGGGTTAGCAACCGATTTGAAATTACCCTCTCAACATCACATGCAGAAGTTGGTGTTTCAACCTAAATGCGAGTGA

Protein sequence

MSLAPQMEAATKEVEDKNTDEKAMAVNDKAPVRPRGRPFFQRRWTTLDKRSAGLLLVLHLLCLLAPFHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTLQGDPIDWVSTHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEERNRKRKRFRNIFVGCLKYGRPNNVEDLQKQAFYRFLRTTNILHHFGLAIVLNAVGGLPFLVWGMRPCWHTAIALVKKAFSKKCKNGMLSTCHISLNSIGHMWGNQQWNTGDQSRNNWVLALLIFGEGWHNHHHAFEYSARHGLEWWQLDVTWWVILLLQALGLATDLKLPSQHHMQKLVFQPKCE
Homology
BLAST of CmaCh01G001480 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 357.1 bits (915), Expect = 2.4e-97
Identity = 173/325 (53.23%), Postives = 213/325 (65.54%), Query Frame = 0

Query: 39  FFQRRWTTLDKRSAGLLLVLHLLCLLAPFHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLS 98
           +++R W  +D  +  ++L +HLL LLAPF F W A  +AF LY  TGL G+TLS+HR+LS
Sbjct: 93  WWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLS 152

Query: 99  HKSFKLPKWLEYTFAYIGVLTLQGDPIDWVSTHRYHHQFVDTDRDPHSPKQGFWFGHIAW 158
           HK+FKLPKWLEY FAY G   LQG+PIDWVSTHRYHHQF D+DRDPHSP  GFWF H+ W
Sbjct: 153 HKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNW 212

Query: 159 IFDSYGLTKKVCPKHVEDFEERNRKRKRFRNIFVGCLKYGRPNNVEDLQKQAFYRFLRTT 218
           +FD+  +T++                             G PNNV DL+KQ FYRFLRTT
Sbjct: 213 MFDTNTITQRC----------------------------GEPNNVGDLEKQPFYRFLRTT 272

Query: 219 NILHHFGLAIVLNAVGGLPFLVWGM--RPCWHTAIALVKKAFSKKCKNGMLSTCHIS--L 278
            ILH   LA+ L A+GG PF+VWGM  R  W                       HI+  +
Sbjct: 273 YILHPLALAVALYAMGGFPFIVWGMGVRIVW---------------------VYHITWLV 332

Query: 279 NSIGHMWGNQQWNTGDQSRNNWVLALLIFGEGWHNHHHAFEYSARHGLEWWQLDVTWWVI 338
           NS  H+WG Q WNTGD S+NNW +A L FGEGWHN+HHAFE+SARHGLEWWQLD+TW+V+
Sbjct: 333 NSACHVWGKQAWNTGDLSKNNWWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVV 368

Query: 339 LLLQALGLATDLKLPSQHHMQKLVF 360
             LQA+GLATD+KLPS+   Q++ F
Sbjct: 393 KFLQAIGLATDVKLPSEAQKQRMAF 368

BLAST of CmaCh01G001480 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 1.6e-85
Identity = 156/344 (45.35%), Postives = 213/344 (61.92%), Query Frame = 0

Query: 16  DKNTDEKAMAVNDKAPVRPRGRPFFQRRWTTLDKRSAGLLLVLHLLCLLAPFHFTWSAFC 75
           +K  + K MA  DKA +  + R  ++R+W  LD   A   L +H LCLLAPF+FTW A  
Sbjct: 7   EKEENNKKMAA-DKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALR 66

Query: 76  LAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTLQGDPIDWVSTHRYHH 135
           +A ++Y   GL G+T+SYHR+L+H+SFK+PKWLEY FAY G+L +QGDPIDWVSTHRYHH
Sbjct: 67  VALIVYTVGGL-GITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHH 126

Query: 136 QFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEERNRKRKRFRNIFVGCL 195
           QF D+DRDPHSP +GFWF H+ W+FD+  L +K                           
Sbjct: 127 QFTDSDRDPHSPNEGFWFSHLLWLFDTGYLVEKC-------------------------- 186

Query: 196 KYGRPNNVEDLQKQAFYRFLRTTNILHHFGLAIVLNAVGGLPFLVWGMRPCWHTAIALVK 255
             GR  NVEDL++Q +Y+FL+ T + H      +L   GGL FL WGM            
Sbjct: 187 --GRRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGM------------ 246

Query: 256 KAFSKKCKNGMLSTCHIS--LNSIGHMWGNQQWNTGDQSRNNWVLALLIFGEGWHNHHHA 315
                    G+    H++  +NS+ H+WG++ W T D SRN W L++  FGE WHN+HHA
Sbjct: 247 -------GIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRNVWWLSVFSFGESWHNNHHA 301

Query: 316 FEYSARHGLEWWQLDVTWWVILLLQALGLATDLKLPSQHHMQKL 358
           FE SAR GLEWWQ+D++W+++  L+ +GLATD+KLPS+   +++
Sbjct: 307 FESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQRRRM 301

BLAST of CmaCh01G001480 vs. ExPASy Swiss-Prot
Match: Q9FV68 (Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 1.6e-85
Identity = 160/324 (49.38%), Postives = 207/324 (63.89%), Query Frame = 0

Query: 35  RGRPFFQRRWTTLDKRSAGLLLVLHLLCLLAPFHFTWSAFCLAFLLYNF-TGLFGLTLSY 94
           + + F++R+W   D ++A  LLVLH L  +APF+F+W AF ++F+L  F +G+ G+TL +
Sbjct: 72  KAKGFWRRKWNPRDIQNAVTLLVLHALAAMAPFYFSWDAFWISFILLGFASGVLGITLCF 131

Query: 95  HRHLSHKSFKLPKWLEYTFAYIGVLTLQGDPIDWVSTHRYHHQFVDTDRDPHSPKQGFWF 154
           HR L+H  FKLPK +EY FAY G L LQGDP++WVS HRYHHQFVDT+RD HSP QGFWF
Sbjct: 132 HRCLTHGGFKLPKLVEYFFAYCGSLALQGDPMEWVSNHRYHHQFVDTERDVHSPTQGFWF 191

Query: 155 GHIAWIFDSYGLTKKVCPKHVEDFEERNRKRKRFRNIFVGCLKYGRPNNVEDLQKQAFYR 214
            HI W+ D                          +++FV   + GR NNV DL+KQAFYR
Sbjct: 192 CHIGWVLD--------------------------KDLFVE-KRGGRRNNVNDLKKQAFYR 251

Query: 215 FLRTTNILHHFGLAIVLNAVGGLPFLVWGMRPCWHTAIALVKKAFSKKCKNGMLSTCHIS 274
           FL+ T + H   L  +L  VGG P++VWGM         LV    S             +
Sbjct: 252 FLQKTYMYHQLALIALLYYVGGFPYIVWGM------GFRLVFMFHST-----------FA 311

Query: 275 LNSIGHMWGNQQWNTGDQSRNNWVLALLIFGEGWHNHHHAFEYSARHGLEWWQLDVTWWV 334
           +NS+ H WG + WNTGD S NN  +AL  FGEGWHN+HHAFE SARHGLEWW++DVTW+V
Sbjct: 312 INSVCHKWGGRPWNTGDLSTNNMFVALCAFGEGWHNNHHAFEQSARHGLEWWEIDVTWYV 351

Query: 335 ILLLQALGLATDLKLPSQHHMQKL 358
           I  LQA+GLAT++KLP++   QKL
Sbjct: 372 IRTLQAIGLATNVKLPTEAQKQKL 351

BLAST of CmaCh01G001480 vs. ExPASy Swiss-Prot
Match: P0DOW3 (Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 5.2e-84
Identity = 158/321 (49.22%), Postives = 201/321 (62.62%), Query Frame = 0

Query: 42  RRWTTLDKRSAGLLLVLHLLCLLAPFHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKS 101
           R W + D     L+  LH+LCL APF F+WSAF L+  LY   G+FG TLSYHR+L+H+S
Sbjct: 35  RVWNSTDIFHIILVGGLHVLCLSAPFTFSWSAFWLSLTLYAVCGVFGTTLSYHRNLTHRS 94

Query: 102 FKLPKWLEYTFAYIGVLTLQGDPIDWVSTHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFD 161
           FKLPK+LEY FAY+G+  LQGDP+ WVSTHRYHH+F DT  DPHSP +GFWF HI W+FD
Sbjct: 95  FKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKFTDTYLDPHSPIEGFWFSHIFWLFD 154

Query: 162 SYGLTKKVCPKHVEDFEERNRKRKRFRNIFVGCLKYGRPNNVEDLQKQAFYRFLRTTNIL 221
           S                         + I   C   GR  N  DL KQ++YRFL  T + 
Sbjct: 155 S-------------------------KYILEEC---GRYENAGDLLKQSYYRFLERTFVF 214

Query: 222 HHFGLAIVLNAVGGLPFLVWGM--RPCWHTAIALVKKAFSKKCKNGMLSTCHISLNSIGH 281
           H +  A +L   GG PF+VWGM  R  W    + +                   +NS+ H
Sbjct: 215 HVYLQAALLYMFGGFPFIVWGMAVRTVWGFHASWL-------------------VNSVCH 274

Query: 282 MWGNQQWNTGDQSRNNWVLALLIFGEGWHNHHHAFEYSARHGLEWWQLDVTWWVILLLQA 341
            +G+Q W+TGD S NNW +A+L  GEGWHN+HHAFEYSARHG+EWWQ+D TW+VI LL+ 
Sbjct: 275 RYGHQAWDTGDLSTNNWFIAMLTSGEGWHNNHHAFEYSARHGIEWWQIDTTWYVIKLLEY 308

Query: 342 LGLATDLKLPSQHHMQKLVFQ 361
           LGLATD+K+PS+ H +K+ F+
Sbjct: 335 LGLATDVKVPSEVHKRKMSFK 308

BLAST of CmaCh01G001480 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 6.8e-84
Identity = 156/335 (46.57%), Postives = 209/335 (62.39%), Query Frame = 0

Query: 29  KAPVRPRGRPF-FQRRWTTLDKRSAGLLLVLHLLCLLAPFHFTWSAFCLAFLLYNFTGLF 88
           + PV+ + +   + R+W + D      +  LH+LCL AP  F+W +F + F LY   G+F
Sbjct: 24  EVPVKEKTKKITWVRKWNSTDVFHILFIGGLHVLCLFAPSTFSWKSFWVCFALYAICGVF 83

Query: 89  GLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTLQGDPIDWVSTHRYHHQFVDTDRDPHSP 148
           G TLS+HR+L+H+SFKLPK+LEY FAY+G+  LQGDP+ WVSTHRYHH++ DT  DPHSP
Sbjct: 84  GTTLSFHRNLTHRSFKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKYTDTYLDPHSP 143

Query: 149 KQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEERNRKRKRFRNIFVGCLKYGRPNNVEDLQ 208
            +GFWF HI W+FDS         K++ +                   K GR  N  DL 
Sbjct: 144 IEGFWFCHIFWLFDS---------KYIIE-------------------KCGRYENAGDLM 203

Query: 209 KQAFYRFLRTTNILHHFGLAIVLNAVGGLPFLVWGMRPCWHTAIALVKKAFSKKCKNGML 268
           KQ++YRFL  T + H +  A +L   GG PF+VWGM     T +                
Sbjct: 204 KQSYYRFLERTFVYHVYLQAALLYLFGGFPFIVWGM--AVRTILGF-------------- 263

Query: 269 STCHIS--LNSIGHMWGNQQWNTGDQSRNNWVLALLIFGEGWHNHHHAFEYSARHGLEWW 328
              H+S  +NS+ H WGN+ WNTGD S NNW +A+L  GEGWHN+HHAFEYSARHG+EWW
Sbjct: 264 ---HLSWLVNSVCHRWGNRPWNTGDLSTNNWFIAMLTSGEGWHNNHHAFEYSARHGIEWW 311

Query: 329 QLDVTWWVILLLQALGLATDLKLPSQHHMQKLVFQ 361
           Q+D TW++I LL+ LGLATD+K+PS+ H +K+ F+
Sbjct: 324 QIDTTWYIIKLLEYLGLATDIKVPSEIHKRKMSFK 311

BLAST of CmaCh01G001480 vs. ExPASy TrEMBL
Match: A0A6J1GCG5 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111452890 PE=3 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 5.1e-191
Identity = 325/362 (89.78%), Postives = 330/362 (91.16%), Query Frame = 0

Query: 7   MEAATKEVEDKNTDEKAMAVNDKAPVRPRGRPFFQRRWTTLDKRSAGLLLVLHLLCLLAP 66
           MEAATKEVEDKN DEKAMAVNDKAPVRPRGRPFFQRRWTTLDKRSAGLLL LHLLCLLAP
Sbjct: 1   MEAATKEVEDKNNDEKAMAVNDKAPVRPRGRPFFQRRWTTLDKRSAGLLLFLHLLCLLAP 60

Query: 67  FHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTLQGDPID 126
           FHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTLQGDPID
Sbjct: 61  FHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTLQGDPID 120

Query: 127 WVSTHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEERNRKRKR 186
           W+STHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEERNRK KR
Sbjct: 121 WMSTHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEERNRKPKR 180

Query: 187 FRNIFVGCLKYGRPNNVEDLQKQAFYRFLRTTNILHHFGLAIVLNAVGGLPFLVWGMRPC 246
           FRNIFVGCLKYGRPNNVEDLQKQAFYRFLRTT ILHHFGLAIVL AVGGLPFLVWGM   
Sbjct: 181 FRNIFVGCLKYGRPNNVEDLQKQAFYRFLRTTYILHHFGLAIVLYAVGGLPFLVWGM--- 240

Query: 247 WHTAIALVKKAFSKKCKNGMLSTC--HI--SLNSIGHMWGNQQWNTGDQSRNNWVLALLI 306
                             G+ + C  H+  SLNSIGHMWGNQQWNTGDQSRNNWVLALLI
Sbjct: 241 ------------------GVRTVCFQHVTFSLNSIGHMWGNQQWNTGDQSRNNWVLALLI 300

Query: 307 FGEGWHNHHHAFEYSARHGLEWWQLDVTWWVILLLQALGLATDLKLPSQHHMQKLVFQPK 365
           FGEGWHN+HHAFEYSARHGLEWWQLDVTWWVILLLQALGLATDLKLPSQHHMQKLVFQPK
Sbjct: 301 FGEGWHNNHHAFEYSARHGLEWWQLDVTWWVILLLQALGLATDLKLPSQHHMQKLVFQPK 341

BLAST of CmaCh01G001480 vs. ExPASy TrEMBL
Match: A0A6J1KHF9 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111493270 PE=3 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 2.6e-179
Identity = 307/366 (83.88%), Postives = 319/366 (87.16%), Query Frame = 0

Query: 1   MSLAPQMEAATKEVEDKNTDEKAMAVNDKAPVRPRGRPFFQRRWTTLDKRSAGLLLVLHL 60
           MSLAPQMEAATKEVEDKNTDEKAMAVNDKAPVRPRGRPFFQRRWTTLDKRSAGLLLVLHL
Sbjct: 1   MSLAPQMEAATKEVEDKNTDEKAMAVNDKAPVRPRGRPFFQRRWTTLDKRSAGLLLVLHL 60

Query: 61  LCLLAPFHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTL 120
           LCLLAPFHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTL
Sbjct: 61  LCLLAPFHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTL 120

Query: 121 QGDPIDWVSTHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEER 180
           QGDPIDWVSTHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEER
Sbjct: 121 QGDPIDWVSTHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEER 180

Query: 181 NRKRKRFRNIFVGCLKYGRPNNVEDLQKQAFYRFLRTTNILHHFGLAIVLNAVGGLPFLV 240
           NRKRKRFRNIFVGCLKYGRPNNVEDLQKQAFYRFLRTTNILHHFGLAIVLNAVGGLPFLV
Sbjct: 181 NRKRKRFRNIFVGCLKYGRPNNVEDLQKQAFYRFLRTTNILHHFGLAIVLNAVGGLPFLV 240

Query: 241 WGM--RPCWHTAIALVKKAFSKKCKNGMLSTCHIS--LNSIGHMWGNQQWNTGDQSRNNW 300
           WGM  R  W                       HI+  +NS  H+WG + W+T D S+NNW
Sbjct: 241 WGMGVRLVW---------------------VLHITFFVNSACHIWGYRLWDTNDLSKNNW 300

Query: 301 VLALLIFGEGWHNHHHAFEYSARHGLEWWQLDVTWWVILLLQALGLATDLKLPSQHHMQK 360
           V+ LL FGEGWHN+HHAFEYS+RHG EWW+ D+TW VIL LQALGLATD+KLPS  H QK
Sbjct: 301 VIGLLAFGEGWHNNHHAFEYSSRHGHEWWEFDLTWCVILFLQALGLATDVKLPSPRHQQK 345

Query: 361 LVFQPK 363
           L   PK
Sbjct: 361 LALNPK 345

BLAST of CmaCh01G001480 vs. ExPASy TrEMBL
Match: A0A6J1KE40 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111493021 PE=3 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 1.5e-163
Identity = 279/360 (77.50%), Postives = 306/360 (85.00%), Query Frame = 0

Query: 7   MEAATKEVEDKNTDEKAMAVNDKAPVRPRGRPFFQRRWTTLDKRSAGLLLVLHLLCLLAP 66
           MEA  KE+ED N D+KAMAV D+APVR   RP F+R+WT++DKR+AG +L +HLLCLLAP
Sbjct: 1   MEAIKKELEDTNNDQKAMAVEDEAPVRSGQRPIFKRKWTSVDKRTAGAVLAMHLLCLLAP 60

Query: 67  FHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTLQGDPID 126
           FHFTW AF LA +LY  TGLFGLTLSYHRHL+HKSFKLPKWLEYTFAYIGV T QGDPID
Sbjct: 61  FHFTWPAFWLAVVLYIITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTYQGDPID 120

Query: 127 WVSTHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEERNRKRKR 186
           WVSTHRYHHQFVDT+RDPH+P QGFWFGH+AWIFDSYGLTKKV PKHVE+FEERNRK K 
Sbjct: 121 WVSTHRYHHQFVDTERDPHTPTQGFWFGHVAWIFDSYGLTKKVSPKHVENFEERNRKGKL 180

Query: 187 FRNIFVGCLKYGRPNNVEDLQKQAFYRFLRTTNILHHFGLAIVLNAVGGLPFLVWGMRPC 246
           FRNIFVG LKYGRPNNVEDLQKQAFYRFLR T ILHH GLA+VL AVGGLPFLVWGM   
Sbjct: 181 FRNIFVGYLKYGRPNNVEDLQKQAFYRFLRRTYILHHVGLAVVLYAVGGLPFLVWGM--- 240

Query: 247 WHTAIALVKKAFSKKCKNGMLSTCHI--SLNSIGHMWGNQQWNTGDQSRNNWVLALLIFG 306
               + +V             S  HI  ++NS+GHMWGNQQWNTGDQSRNNWVLALLIFG
Sbjct: 241 ---GVRIV-------------SFQHITFAVNSLGHMWGNQQWNTGDQSRNNWVLALLIFG 300

Query: 307 EGWHNHHHAFEYSARHGLEWWQLDVTWWVILLLQALGLATDLKLPSQHHMQKLVFQPKCE 365
           EGWHN+HHAFEYSARHGLEWWQLDVTWWVIL+L+A+GLATD+KLPSQHHMQKL  QPKCE
Sbjct: 301 EGWHNNHHAFEYSARHGLEWWQLDVTWWVILVLEAMGLATDVKLPSQHHMQKLGMQPKCE 341

BLAST of CmaCh01G001480 vs. ExPASy TrEMBL
Match: A0A6J1DKS5 (acyl-CoA C20 Delta5-desaturase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111020979 PE=3 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 2.5e-161
Identity = 277/368 (75.27%), Postives = 305/368 (82.88%), Query Frame = 0

Query: 1   MSLAPQMEAATKEVEDKNTDEKAMAVNDKAPVRPRGRPFFQRRWTTLDKRSAGLLLVLHL 60
           M LAP+ME   + +EDKN D+KA+   D+A VR R RP F+R+WT+LDKRSAG LLV+HL
Sbjct: 1   MLLAPEMEETRRLLEDKNNDQKAVVGEDEAAVRDRRRPIFRRKWTSLDKRSAGALLVMHL 60

Query: 61  LCLLAPFHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTL 120
           LCLLAPF FTW AF LAF+LY  TGLFGLTLSYHRHL+HKSFKLPKWLEYTFAYIGV T 
Sbjct: 61  LCLLAPFQFTWPAFWLAFVLYIITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTF 120

Query: 121 QGDPIDWVSTHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEER 180
           QGDPIDWVSTHRYHHQFVDT+RDPH+P QGFWFGHIAWI+DSYGLTKKVCPK+V DFE R
Sbjct: 121 QGDPIDWVSTHRYHHQFVDTERDPHTPTQGFWFGHIAWIWDSYGLTKKVCPKYVRDFEVR 180

Query: 181 NRKRKRFRNIFVGCLKYGRPNNVEDLQKQAFYRFLRTTNILHHFGLAIVLNAVGGLPFLV 240
           + KR R RN+FVG LKYGRPNN EDLQKQAFYRFLR T ILHH GLA+VL AVGGLPFLV
Sbjct: 181 DGKRSRLRNMFVGYLKYGRPNNAEDLQKQAFYRFLRRTYILHHVGLAVVLYAVGGLPFLV 240

Query: 241 WGMRPCWHTAIALVKKAFSKKCKNGMLSTC--HI--SLNSIGHMWGNQQWNTGDQSRNNW 300
           WGM                     G+ + C  H+  S+NS+GHMWGNQQWNTGDQSRNNW
Sbjct: 241 WGM---------------------GVRTVCFQHVTFSVNSLGHMWGNQQWNTGDQSRNNW 300

Query: 301 VLALLIFGEGWHNHHHAFEYSARHGLEWWQLDVTWWVILLLQALGLATDLKLPSQHHMQK 360
           VLA+LIFGEGWHN+HHAFEYSARHGLEWWQLDVTWWVILLLQALGLATD+KLPSQ+HMQK
Sbjct: 301 VLAMLIFGEGWHNNHHAFEYSARHGLEWWQLDVTWWVILLLQALGLATDVKLPSQNHMQK 347

Query: 361 LVFQPKCE 365
           L  QPK E
Sbjct: 361 LAIQPKYE 347

BLAST of CmaCh01G001480 vs. ExPASy TrEMBL
Match: A0A5A7TMC1 (Delta-9 acyl-lipid desaturase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001850 PE=3 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 5.5e-161
Identity = 276/362 (76.24%), Postives = 299/362 (82.60%), Query Frame = 0

Query: 7   MEAATKEVEDKNTDEKAMAVNDKAPVRPRGRPFFQRRWTTLDKRSAGLLLVLHLLCLLAP 66
           ME   KE+EDKN+D+KAMAV DKA VR R RPFF+R+WTTLDKRSAG  LV+H LCLLAP
Sbjct: 1   METIRKELEDKNSDQKAMAVGDKARVRSRRRPFFRRKWTTLDKRSAGAFLVMHFLCLLAP 60

Query: 67  FHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTLQGDPID 126
           F+FTW AF LA  LY  TGLFGLTLSYHRHL+HKSFKLPKWLEYTFAYIGV TLQGDPID
Sbjct: 61  FYFTWPAFWLAVALYIITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTLQGDPID 120

Query: 127 WVSTHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEERNRKRKR 186
           WVSTHRYHHQFVDT+RDPH+P QGF FGHIAWIFDSYGLTKKVCPKHVEDFE ++ KRK 
Sbjct: 121 WVSTHRYHHQFVDTERDPHTPTQGFLFGHIAWIFDSYGLTKKVCPKHVEDFEAKDEKRKS 180

Query: 187 FRNIFVGCLKYGRPNNVEDLQKQAFYRFLRTTNILHHFGLAIVLNAVGGLPFLVWGMRPC 246
            +N+FVG LKYGRPNN EDLQKQAFYRFLR T ILHHFGLAI+L AVGGLPFL WGM   
Sbjct: 181 TKNMFVGYLKYGRPNNAEDLQKQAFYRFLRRTYILHHFGLAIILYAVGGLPFLTWGM--- 240

Query: 247 WHTAIALVKKAFSKKCKNGMLSTC--HI--SLNSIGHMWGNQQWNTGDQSRNNWVLALLI 306
                             G+ + C  H+  ++NS+GHMWGNQ WNTGDQSRNNWVLALLI
Sbjct: 241 ------------------GVRTVCFQHVTFAVNSLGHMWGNQPWNTGDQSRNNWVLALLI 300

Query: 307 FGEGWHNHHHAFEYSARHGLEWWQLDVTWWVILLLQALGLATDLKLPSQHHMQKLVFQPK 365
           FGEGWHN+HHAFEYSARHGLEWWQ DVTWWVIL LQALGLATDLKLPS+HHM KL  QPK
Sbjct: 301 FGEGWHNNHHAFEYSARHGLEWWQFDVTWWVILFLQALGLATDLKLPSKHHMHKLAIQPK 341

BLAST of CmaCh01G001480 vs. NCBI nr
Match: XP_023524612.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 679.5 bits (1752), Expect = 1.6e-191
Identity = 328/367 (89.37%), Postives = 334/367 (91.01%), Query Frame = 0

Query: 1   MSLAPQMEAATKEVEDKNTDEKAMAVNDKAPVRPRGRPFFQRRWTTLDKRSAGLLLVLHL 60
           MSLAPQMEAATKEVE KN DEKAMAVNDKA VRPRGRPFFQRRWTTLDKRSAGLLLVLHL
Sbjct: 1   MSLAPQMEAATKEVEHKNDDEKAMAVNDKASVRPRGRPFFQRRWTTLDKRSAGLLLVLHL 60

Query: 61  LCLLAPFHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTL 120
           LCLLAPFHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTL
Sbjct: 61  LCLLAPFHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTL 120

Query: 121 QGDPIDWVSTHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEER 180
           QGDPIDWVSTHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEER
Sbjct: 121 QGDPIDWVSTHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEER 180

Query: 181 NRKRKRFRNIFVGCLKYGRPNNVEDLQKQAFYRFLRTTNILHHFGLAIVLNAVGGLPFLV 240
           NRKRKRFRNIFVGCLKYGRPNN+EDLQKQAFYRFLRTT ILHHFGLAIVL AVGGLPFLV
Sbjct: 181 NRKRKRFRNIFVGCLKYGRPNNLEDLQKQAFYRFLRTTYILHHFGLAIVLYAVGGLPFLV 240

Query: 241 WGMRPCWHTAIALVKKAFSKKCKNGMLSTC--HI--SLNSIGHMWGNQQWNTGDQSRNNW 300
           WGM                     G+ + C  H+  SLNSIGHMWGNQQWNTGDQSRNNW
Sbjct: 241 WGM---------------------GVRTVCFQHVTFSLNSIGHMWGNQQWNTGDQSRNNW 300

Query: 301 VLALLIFGEGWHNHHHAFEYSARHGLEWWQLDVTWWVILLLQALGLATDLKLPSQHHMQK 360
           VLALLIFGEGWHN+HHAFEYSARHGLEWWQLDVTWWVILLLQALGLATDLKLPSQHHMQK
Sbjct: 301 VLALLIFGEGWHNNHHAFEYSARHGLEWWQLDVTWWVILLLQALGLATDLKLPSQHHMQK 346

Query: 361 LVFQPKC 364
           LVF+ KC
Sbjct: 361 LVFRRKC 346

BLAST of CmaCh01G001480 vs. NCBI nr
Match: XP_022949578.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 676.8 bits (1745), Expect = 1.1e-190
Identity = 325/362 (89.78%), Postives = 330/362 (91.16%), Query Frame = 0

Query: 7   MEAATKEVEDKNTDEKAMAVNDKAPVRPRGRPFFQRRWTTLDKRSAGLLLVLHLLCLLAP 66
           MEAATKEVEDKN DEKAMAVNDKAPVRPRGRPFFQRRWTTLDKRSAGLLL LHLLCLLAP
Sbjct: 1   MEAATKEVEDKNNDEKAMAVNDKAPVRPRGRPFFQRRWTTLDKRSAGLLLFLHLLCLLAP 60

Query: 67  FHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTLQGDPID 126
           FHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTLQGDPID
Sbjct: 61  FHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTLQGDPID 120

Query: 127 WVSTHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEERNRKRKR 186
           W+STHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEERNRK KR
Sbjct: 121 WMSTHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEERNRKPKR 180

Query: 187 FRNIFVGCLKYGRPNNVEDLQKQAFYRFLRTTNILHHFGLAIVLNAVGGLPFLVWGMRPC 246
           FRNIFVGCLKYGRPNNVEDLQKQAFYRFLRTT ILHHFGLAIVL AVGGLPFLVWGM   
Sbjct: 181 FRNIFVGCLKYGRPNNVEDLQKQAFYRFLRTTYILHHFGLAIVLYAVGGLPFLVWGM--- 240

Query: 247 WHTAIALVKKAFSKKCKNGMLSTC--HI--SLNSIGHMWGNQQWNTGDQSRNNWVLALLI 306
                             G+ + C  H+  SLNSIGHMWGNQQWNTGDQSRNNWVLALLI
Sbjct: 241 ------------------GVRTVCFQHVTFSLNSIGHMWGNQQWNTGDQSRNNWVLALLI 300

Query: 307 FGEGWHNHHHAFEYSARHGLEWWQLDVTWWVILLLQALGLATDLKLPSQHHMQKLVFQPK 365
           FGEGWHN+HHAFEYSARHGLEWWQLDVTWWVILLLQALGLATDLKLPSQHHMQKLVFQPK
Sbjct: 301 FGEGWHNNHHAFEYSARHGLEWWQLDVTWWVILLLQALGLATDLKLPSQHHMQKLVFQPK 341

BLAST of CmaCh01G001480 vs. NCBI nr
Match: XP_022998683.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 637.9 bits (1644), Expect = 5.4e-179
Identity = 307/366 (83.88%), Postives = 319/366 (87.16%), Query Frame = 0

Query: 1   MSLAPQMEAATKEVEDKNTDEKAMAVNDKAPVRPRGRPFFQRRWTTLDKRSAGLLLVLHL 60
           MSLAPQMEAATKEVEDKNTDEKAMAVNDKAPVRPRGRPFFQRRWTTLDKRSAGLLLVLHL
Sbjct: 1   MSLAPQMEAATKEVEDKNTDEKAMAVNDKAPVRPRGRPFFQRRWTTLDKRSAGLLLVLHL 60

Query: 61  LCLLAPFHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTL 120
           LCLLAPFHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTL
Sbjct: 61  LCLLAPFHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTL 120

Query: 121 QGDPIDWVSTHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEER 180
           QGDPIDWVSTHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEER
Sbjct: 121 QGDPIDWVSTHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEER 180

Query: 181 NRKRKRFRNIFVGCLKYGRPNNVEDLQKQAFYRFLRTTNILHHFGLAIVLNAVGGLPFLV 240
           NRKRKRFRNIFVGCLKYGRPNNVEDLQKQAFYRFLRTTNILHHFGLAIVLNAVGGLPFLV
Sbjct: 181 NRKRKRFRNIFVGCLKYGRPNNVEDLQKQAFYRFLRTTNILHHFGLAIVLNAVGGLPFLV 240

Query: 241 WGM--RPCWHTAIALVKKAFSKKCKNGMLSTCHIS--LNSIGHMWGNQQWNTGDQSRNNW 300
           WGM  R  W                       HI+  +NS  H+WG + W+T D S+NNW
Sbjct: 241 WGMGVRLVW---------------------VLHITFFVNSACHIWGYRLWDTNDLSKNNW 300

Query: 301 VLALLIFGEGWHNHHHAFEYSARHGLEWWQLDVTWWVILLLQALGLATDLKLPSQHHMQK 360
           V+ LL FGEGWHN+HHAFEYS+RHG EWW+ D+TW VIL LQALGLATD+KLPS  H QK
Sbjct: 301 VIGLLAFGEGWHNNHHAFEYSSRHGHEWWEFDLTWCVILFLQALGLATDVKLPSPRHQQK 345

Query: 361 LVFQPK 363
           L   PK
Sbjct: 361 LALNPK 345

BLAST of CmaCh01G001480 vs. NCBI nr
Match: KAG6606804.1 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 633.3 bits (1632), Expect = 1.3e-177
Identity = 308/362 (85.08%), Postives = 317/362 (87.57%), Query Frame = 0

Query: 7   MEAATKEVEDKNTDEKAMAVNDKAPVRPRGRPFFQRRWTTLDKRSAGLLLVLHLLCLLAP 66
           MEAATKEVEDKN DEKAMAVNDKAPVRPRGRPFFQRRWTTLDKRSAGLLL LHLLCLLAP
Sbjct: 1   MEAATKEVEDKNNDEKAMAVNDKAPVRPRGRPFFQRRWTTLDKRSAGLLLFLHLLCLLAP 60

Query: 67  FHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTLQGDPID 126
           FHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTLQGDPID
Sbjct: 61  FHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTLQGDPID 120

Query: 127 WVSTHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEERNRKRKR 186
           WVSTHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEERNRKRKR
Sbjct: 121 WVSTHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEERNRKRKR 180

Query: 187 FRNIFVGCLKYGRPNNVEDLQKQAFYRFLRTTNILHHFGLAIVLNAVGGLPFLVWGMRPC 246
           FRNIFVGCLKYGRPNNVEDLQKQAFYRFLRTT ILHHFGLAIVL AVGGLPFLVWGM   
Sbjct: 181 FRNIFVGCLKYGRPNNVEDLQKQAFYRFLRTTYILHHFGLAIVLYAVGGLPFLVWGM--- 240

Query: 247 WHTAIALVKKAFSKKCKNGMLSTC--HI--SLNSIGHMWGNQQWNTGDQSRNNWVLALLI 306
                             G+ + C  H+  SLNSIGHMWGNQQWNTGDQSRNNWVLALLI
Sbjct: 241 ------------------GVRTVCFQHVTFSLNSIGHMWGNQQWNTGDQSRNNWVLALLI 300

Query: 307 FGEGWHNHHHAFEYSARHGLEWWQLDVTWWVILLLQALGLATDLKLPSQHHMQKLVFQPK 365
           FGEGWHN+HHAFEYSARHGLEWWQLDVTWWVILLLQALG      +PS    +K + + K
Sbjct: 301 FGEGWHNNHHAFEYSARHGLEWWQLDVTWWVILLLQALG------VPSSKCYEKFLKEEK 335

BLAST of CmaCh01G001480 vs. NCBI nr
Match: XP_038903942.1 (acyl-CoA C20 Delta5-desaturase-like [Benincasa hispida])

HSP 1 Score: 585.9 bits (1509), Expect = 2.4e-163
Identity = 281/369 (76.15%), Postives = 303/369 (82.11%), Query Frame = 0

Query: 1   MSLAPQMEAATKEVEDKNTDEKAMAVNDKAPVRPRG-RPFFQRRWTTLDKRSAGLLLVLH 60
           MSL PQME   KE+EDKN D KAMAV D+    P G RP F+R+WTTLDKRSAG  LV+H
Sbjct: 1   MSLIPQMEGTRKELEDKNIDRKAMAVEDECRAMPPGRRPIFRRKWTTLDKRSAGAFLVMH 60

Query: 61  LLCLLAPFHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLT 120
            LCLLAPFHFTW AF LAF+LY  TGLFGLTLSYHRHL+HKSFKLPKWLEYTFAYIGV T
Sbjct: 61  FLCLLAPFHFTWPAFWLAFVLYVITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHT 120

Query: 121 LQGDPIDWVSTHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEE 180
            QGDPIDWVSTHRYHHQFVDT+RDPH+P QGFWFGHIAWIFDSYGLTKKV PKHVEDFE+
Sbjct: 121 FQGDPIDWVSTHRYHHQFVDTERDPHTPTQGFWFGHIAWIFDSYGLTKKVSPKHVEDFEK 180

Query: 181 RNRKRKRFRNIFVGCLKYGRPNNVEDLQKQAFYRFLRTTNILHHFGLAIVLNAVGGLPFL 240
           R+ KRK   NIFVG +KYGRPNNVEDLQKQAFYRFLR T ILHHFGLA+VL  VGGLPFL
Sbjct: 181 RDEKRKSLSNIFVGYVKYGRPNNVEDLQKQAFYRFLRRTYILHHFGLAVVLYLVGGLPFL 240

Query: 241 VWGMRPCWHTAIALVKKAFSKKCKNGMLSTC--HI--SLNSIGHMWGNQQWNTGDQSRNN 300
           VWGM                     G+ + C  H+  ++NS+GHMWGNQQWNTGDQSRNN
Sbjct: 241 VWGM---------------------GVRTVCFQHVTFAVNSLGHMWGNQQWNTGDQSRNN 300

Query: 301 WVLALLIFGEGWHNHHHAFEYSARHGLEWWQLDVTWWVILLLQALGLATDLKLPSQHHMQ 360
           WVLALLIFGEGWHN+HHAFEYSARHGLEWWQLD TWWVIL L+A+GLATDLKLPSQHHMQ
Sbjct: 301 WVLALLIFGEGWHNNHHAFEYSARHGLEWWQLDATWWVILFLEAVGLATDLKLPSQHHMQ 348

Query: 361 KLVFQPKCE 365
           KL  QPK E
Sbjct: 361 KLAIQPKSE 348

BLAST of CmaCh01G001480 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 357.1 bits (915), Expect = 1.7e-98
Identity = 173/325 (53.23%), Postives = 213/325 (65.54%), Query Frame = 0

Query: 39  FFQRRWTTLDKRSAGLLLVLHLLCLLAPFHFTWSAFCLAFLLYNFTGLFGLTLSYHRHLS 98
           +++R W  +D  +  ++L +HLL LLAPF F W A  +AF LY  TGL G+TLS+HR+LS
Sbjct: 93  WWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLS 152

Query: 99  HKSFKLPKWLEYTFAYIGVLTLQGDPIDWVSTHRYHHQFVDTDRDPHSPKQGFWFGHIAW 158
           HK+FKLPKWLEY FAY G   LQG+PIDWVSTHRYHHQF D+DRDPHSP  GFWF H+ W
Sbjct: 153 HKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNW 212

Query: 159 IFDSYGLTKKVCPKHVEDFEERNRKRKRFRNIFVGCLKYGRPNNVEDLQKQAFYRFLRTT 218
           +FD+  +T++                             G PNNV DL+KQ FYRFLRTT
Sbjct: 213 MFDTNTITQRC----------------------------GEPNNVGDLEKQPFYRFLRTT 272

Query: 219 NILHHFGLAIVLNAVGGLPFLVWGM--RPCWHTAIALVKKAFSKKCKNGMLSTCHIS--L 278
            ILH   LA+ L A+GG PF+VWGM  R  W                       HI+  +
Sbjct: 273 YILHPLALAVALYAMGGFPFIVWGMGVRIVW---------------------VYHITWLV 332

Query: 279 NSIGHMWGNQQWNTGDQSRNNWVLALLIFGEGWHNHHHAFEYSARHGLEWWQLDVTWWVI 338
           NS  H+WG Q WNTGD S+NNW +A L FGEGWHN+HHAFE+SARHGLEWWQLD+TW+V+
Sbjct: 333 NSACHVWGKQAWNTGDLSKNNWWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVV 368

Query: 339 LLLQALGLATDLKLPSQHHMQKLVF 360
             LQA+GLATD+KLPS+   Q++ F
Sbjct: 393 KFLQAIGLATDVKLPSEAQKQRMAF 368

BLAST of CmaCh01G001480 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 317.8 bits (813), Expect = 1.2e-86
Identity = 156/344 (45.35%), Postives = 213/344 (61.92%), Query Frame = 0

Query: 16  DKNTDEKAMAVNDKAPVRPRGRPFFQRRWTTLDKRSAGLLLVLHLLCLLAPFHFTWSAFC 75
           +K  + K MA  DKA +  + R  ++R+W  LD   A   L +H LCLLAPF+FTW A  
Sbjct: 7   EKEENNKKMAA-DKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALR 66

Query: 76  LAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTLQGDPIDWVSTHRYHH 135
           +A ++Y   GL G+T+SYHR+L+H+SFK+PKWLEY FAY G+L +QGDPIDWVSTHRYHH
Sbjct: 67  VALIVYTVGGL-GITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHH 126

Query: 136 QFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEERNRKRKRFRNIFVGCL 195
           QF D+DRDPHSP +GFWF H+ W+FD+  L +K                           
Sbjct: 127 QFTDSDRDPHSPNEGFWFSHLLWLFDTGYLVEKC-------------------------- 186

Query: 196 KYGRPNNVEDLQKQAFYRFLRTTNILHHFGLAIVLNAVGGLPFLVWGMRPCWHTAIALVK 255
             GR  NVEDL++Q +Y+FL+ T + H      +L   GGL FL WGM            
Sbjct: 187 --GRRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGM------------ 246

Query: 256 KAFSKKCKNGMLSTCHIS--LNSIGHMWGNQQWNTGDQSRNNWVLALLIFGEGWHNHHHA 315
                    G+    H++  +NS+ H+WG++ W T D SRN W L++  FGE WHN+HHA
Sbjct: 247 -------GIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRNVWWLSVFSFGESWHNNHHA 301

Query: 316 FEYSARHGLEWWQLDVTWWVILLLQALGLATDLKLPSQHHMQKL 358
           FE SAR GLEWWQ+D++W+++  L+ +GLATD+KLPS+   +++
Sbjct: 307 FESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQRRRM 301

BLAST of CmaCh01G001480 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 306.6 bits (784), Expect = 2.7e-83
Identity = 155/351 (44.16%), Postives = 207/351 (58.97%), Query Frame = 0

Query: 9   AATKEVEDKNTDEKAMAVNDKAPVRPRGRPFFQRRWTTLDKRSAGLLLVLHLLCLLAPFH 68
           +  +E   KN    A AV +K   + R   F+ RRW  LD         +H L LLAPF+
Sbjct: 5   STVEENHQKNPSTPA-AVEEK---KKRRWVFWDRRWRRLDYVKFSASFTVHSLALLAPFY 64

Query: 69  FTWSAFCLAFLLYNFTGLFGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTLQGDPIDWV 128
           FTWSA  + FL Y   GL G+T+SYHR+L+H+SFK+PKWLEY  AY  +L +QGDPIDWV
Sbjct: 65  FTWSALWVTFLFYTIGGL-GITVSYHRNLAHRSFKVPKWLEYLLAYCALLAIQGDPIDWV 124

Query: 129 STHRYHHQFVDTDRDPHSPKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEERNRKRKRFR 188
           STHRYHHQF D++RDPHSPK+GFWF H+ WI+DS  L  K                    
Sbjct: 125 STHRYHHQFTDSERDPHSPKEGFWFSHLLWIYDSAYLVSKC------------------- 184

Query: 189 NIFVGCLKYGRPNNVEDLQKQAFYRFLRTTNILHHFGLAIVLNAVGGLPFLVWGMRPCWH 248
                    GR  NVEDL++Q FYRFL+ T + H  GL   L  +GG+ F+ WGM     
Sbjct: 185 ---------GRRANVEDLKRQWFYRFLQKTVLFHILGLGFFLFYLGGMSFVTWGMGV--- 244

Query: 249 TAIALVKKAFSKKCKNGMLSTCHIS--LNSIGHMWGNQQWNTGDQSRNNWVLALLIFGEG 308
                           G     H++  +NS+ H+WG + W T D SRN W L++  FGE 
Sbjct: 245 ----------------GAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVFSFGES 303

Query: 309 WHNHHHAFEYSARHGLEWWQLDVTWWVILLLQALGLATDLKLPSQHHMQKL 358
           WHN+HHAFE SAR GLEWWQ+D++W+++   + +GLATD+K+P++   +++
Sbjct: 305 WHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVPTEAQRRRM 303

BLAST of CmaCh01G001480 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 302.0 bits (772), Expect = 6.5e-82
Identity = 150/319 (47.02%), Postives = 195/319 (61.13%), Query Frame = 0

Query: 37  RPFFQRRWTTLDKRSAGLLLVLHLLCLLAPFHFTWSAFCLAFLLYNFTGLFGLTLSYHRH 96
           R F++R WT+ D     + +  HLL LLAPF+F+W AF +   L    G+  +TLSYHR+
Sbjct: 84  RSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGIC-ITLSYHRN 143

Query: 97  LSHKSFKLPKWLEYTFAYIGVLTLQGDPIDWVSTHRYHHQFVDTDRDPHSPKQGFWFGHI 156
           LSH+SF LPKWLEY FAY GVL  QGDPI+WVS HRYHH+  +T RDPHSP QGFWF H+
Sbjct: 144 LSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGFWFSHM 203

Query: 157 AWIFDSYGLTKKVCPKHVEDFEERNRKRKRFRNIFVGCLKYGRPNNVEDLQKQAFYRFLR 216
           AWIFD+                          +I   C   G   NV+DL +Q FYRFL+
Sbjct: 204 AWIFDT-------------------------SSILENC---GGEENVDDLVRQPFYRFLQ 263

Query: 217 TTNILHHFGLAIVLNAVGGLPFLVWGMRPCWHTAIALVKKAFSKKCKNGMLSTCHISLNS 276
            T +LH    + +    GG+P LVWG+       + L                    +NS
Sbjct: 264 RTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLT-----------------FLVNS 323

Query: 277 IGHMWGNQQWNTGDQSRNNWVLALLIFGEGWHNHHHAFEYSARHGLEWWQLDVTWWVILL 336
           + H+WG + WNT D S+NNW +A+L  GEGWHN+HHAFE+SARHGLEWWQLD+TW +I  
Sbjct: 324 VCHIWGTRAWNTSDFSKNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRF 356

Query: 337 LQALGLATDLKLPSQHHMQ 356
           L+A+GLAT++KLP++  M+
Sbjct: 384 LEAIGLATNVKLPTETQMK 356

BLAST of CmaCh01G001480 vs. TAIR 10
Match: AT1G06100.1 (Fatty acid desaturase family protein )

HSP 1 Score: 295.0 bits (754), Expect = 8.0e-80
Identity = 153/342 (44.74%), Postives = 195/342 (57.02%), Query Frame = 0

Query: 27  NDKAPVRPRGRPFFQRRWTTLDKRSAGLLLVLHLLCLLAPFHFTWSAFCLAFLLYNFTGL 86
           + K  VR   R +  R+WT  D   A  +  +HLLCLLAPF++ W AF    +L   T L
Sbjct: 11  SQKKSVRKEKRAYVLRKWTQFDVGRASTVGTVHLLCLLAPFNYKWEAFRFGIILAILTNL 70

Query: 87  FGLTLSYHRHLSHKSFKLPKWLEYTFAYIGVLTLQGDPIDWVSTHRYHHQFVDTDRDPHS 146
             +T SYHR+L+H+SFKLPKWLEY FAY  +L LQGDP+DWVS HR+HHQF D+DRDPHS
Sbjct: 71  C-ITFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDWVSIHRFHHQFTDSDRDPHS 130

Query: 147 PKQGFWFGHIAWIFDSYGLTKKVCPKHVEDFEERNRKRKRFRNIFVGCLKYGRPNNVEDL 206
           P +GFWF H+ WIFD+  + +K                             GR NNV DL
Sbjct: 131 PIEGFWFSHVLWIFDTDYIREKC----------------------------GRRNNVMDL 190

Query: 207 QKQAFYRFLRTTNILHHFGLAIVLNAVGGLPFLVW--------GMRPCWHTAIALVKKAF 266
           ++Q FYRFL+ T +LH      ++   GGLP+L W        G    W           
Sbjct: 191 KQQWFYRFLKKTLVLHILAFWTLIYLWGGLPYLTWTVGFGGVIGYHGTW----------- 250

Query: 267 SKKCKNGMLSTCHISLNSIGHMWGNQQWNTGDQSRNNWVLALLIFGEGWHNHHHAFEYSA 326
                          +NS  H+ G+Q W T D SRN W LALL  GE WHN+HHAFE SA
Sbjct: 251 --------------LVNSACHICGSQAWQTNDTSRNVWWLALLTMGESWHNNHHAFETSA 298

Query: 327 RHGLEWWQLDVTWWVILLLQALGLATDLKLPSQHHMQKLVFQ 361
           RHGLEW+QLD+TW++I   QALGLAT++KLP+    +K+  +
Sbjct: 311 RHGLEWYQLDITWYLIWFFQALGLATNVKLPTDAQKRKMAIR 298

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949X02.4e-9753.23Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab... [more]
O657971.6e-8545.35Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=... [more]
Q9FV681.6e-8549.38Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1[more]
P0DOW35.2e-8449.22Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1[more]
P0DOW26.8e-8446.57Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GCG55.1e-19189.78palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1KHF92.6e-17983.88palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1KE401.5e-16377.50palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1DKS52.5e-16175.27acyl-CoA C20 Delta5-desaturase-like isoform X1 OS=Momordica charantia OX=3673 GN... [more]
A0A5A7TMC15.5e-16176.24Delta-9 acyl-lipid desaturase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
Match NameE-valueIdentityDescription
XP_023524612.11.6e-19189.37palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C... [more]
XP_022949578.11.1e-19089.78palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C... [more]
XP_022998683.15.4e-17983.88palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C... [more]
KAG6606804.11.3e-17785.08Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic, partia... [more]
XP_038903942.12.4e-16376.15acyl-CoA C20 Delta5-desaturase-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G15850.11.7e-9853.23fatty acid desaturase 5 [more]
AT1G06080.11.2e-8645.35delta 9 desaturase 1 [more]
AT2G31360.12.7e-8344.1616:0delta9 desaturase 2 [more]
AT3G15870.16.5e-8247.02Fatty acid desaturase family protein [more]
AT1G06100.18.0e-8044.74Fatty acid desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015876Acyl-CoA desaturasePRINTSPR00075FACDDSATRASEcoord: 94..114
score: 32.83
coord: 131..160
score: 47.72
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 18..359
IPR015876Acyl-CoA desaturaseCDDcd03505Delta9-FADS-likecoord: 71..347
e-value: 1.42798E-67
score: 208.949
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 70..333
e-value: 8.6E-16
score: 58.5
NoneNo IPR availablePANTHERPTHR11351:SF87FATTY ACID DESATURASE, TYPE 1, CORE-RELATEDcoord: 18..359

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G001480.1CmaCh01G001480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042149 cellular response to glucose starvation
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0006468 protein phosphorylation
biological_process GO:0050790 regulation of catalytic activity
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0031588 nucleotide-activated protein kinase complex
cellular_component GO:0005634 nucleus
molecular_function GO:0009979 16:0 monogalactosyldiacylglycerol desaturase activity
molecular_function GO:0016208 AMP binding
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water
molecular_function GO:0019901 protein kinase binding
molecular_function GO:0019887 protein kinase regulator activity