CmaCh01G001250 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh01G001250
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	caffeoylshikimate esterase
Location	Cma_Chr01: 567526 .. 568500 (-)
RNA-Seq Expression	CmaCh01G001250
Synteny	CmaCh01G001250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCCCGGAACCACCAGCGCCGGAAACTCTGACGAACTACTGGGGCGACCAACCGGAGGAAGAATTCTACGCCTCACAAGGAGTTCGAAACTCCAAATCCTTCTTCGAAACCCCCCATGGCAAGCTCTTCACACAGAGCTTCATTCCTCTGGACTGGCCAGAGCCCAAAGGCACCGTCTACATGACTCACGGCTACGGCTCCGACACCGGCTGGATGTTCCAAAAGATCTGTCTCAGCTATGCCTCTTGGGGCTACGCCGTCTTCGCCGCCGATCTCCTTGGCCATGGCCGCTCCGATGGCCTCCGCTGCTACCTCGGCGATATGGAAAAAGTCGCCGCCGCCTCCCTCTCTTTCTTCCTCCACACCCGCCGCAGCGAGCCCTACTGCCATCTCCCTGCCTTCCTCTTCGGCGAGTCCATGGGCGGTGCCGCCACCATGCTCATGTACTTCCAGTCAGATCCAGACACCTGGACCGGTCTGATCTTCTCGGCGCCGCTTTTTGTCATTCCGGAAAACATGAAGCCGAGCAAACTCCGCCTCTTCCTCTACGGCCTCCTCTTCGGCGTCGCCGACACTTGGGCCGCCATGCCGGACAATAAAATGGTGGGAAAGGCGATTAAGGATCCGGAGAAGTTGAAAATCATAGCGGCGAATCCTAGACGGTACACAGGACCACCGAGGGTTGGGACAATGAGGGAATTGGTTAGAGTTACGCAGTACATTAGAGACAATTTTTCGAGGGTTACGGCGCCATTTCTGACGGTGCACGGGACGGCGGACGGAGTGACGTGTCCGTCGTCGTCGGAATTACTGTACGAGAAGGCGGCGAGCGTGGATAAGACCTTGAAGCTTTACGAGGGGATGTACCATTCGTTAATTCAAGGGGAGCCGGATGAGAATATGGAAATTGTTCTGAGGGATATGAGGGAGTGGATCGACGAGAGGGTTCAGAGATATGGATCGAAGAAATAG

mRNA sequence

ATGGCCCCGGAACCACCAGCGCCGGAAACTCTGACGAACTACTGGGGCGACCAACCGGAGGAAGAATTCTACGCCTCACAAGGAGTTCGAAACTCCAAATCCTTCTTCGAAACCCCCCATGGCAAGCTCTTCACACAGAGCTTCATTCCTCTGGACTGGCCAGAGCCCAAAGGCACCGTCTACATGACTCACGGCTACGGCTCCGACACCGGCTGGATGTTCCAAAAGATCTGTCTCAGCTATGCCTCTTGGGGCTACGCCGTCTTCGCCGCCGATCTCCTTGGCCATGGCCGCTCCGATGGCCTCCGCTGCTACCTCGGCGATATGGAAAAAGTCGCCGCCGCCTCCCTCTCTTTCTTCCTCCACACCCGCCGCAGCGAGCCCTACTGCCATCTCCCTGCCTTCCTCTTCGGCGAGTCCATGGGCGGTGCCGCCACCATGCTCATGTACTTCCAGTCAGATCCAGACACCTGGACCGGTCTGATCTTCTCGGCGCCGCTTTTTGTCATTCCGGAAAACATGAAGCCGAGCAAACTCCGCCTCTTCCTCTACGGCCTCCTCTTCGGCGTCGCCGACACTTGGGCCGCCATGCCGGACAATAAAATGGTGGGAAAGGCGATTAAGGATCCGGAGAAGTTGAAAATCATAGCGGCGAATCCTAGACGGTACACAGGACCACCGAGGGTTGGGACAATGAGGGAATTGGTTAGAGTTACGCAGTACATTAGAGACAATTTTTCGAGGGTTACGGCGCCATTTCTGACGGTGCACGGGACGGCGGACGGAGTGACGTGTCCGTCGTCGTCGGAATTACTGTACGAGAAGGCGGCGAGCGTGGATAAGACCTTGAAGCTTTACGAGGGGATGTACCATTCGTTAATTCAAGGGGAGCCGGATGAGAATATGGAAATTGTTCTGAGGGATATGAGGGAGTGGATCGACGAGAGGGTTCAGAGATATGGATCGAAGAAATAG

Coding sequence (CDS)

ATGGCCCCGGAACCACCAGCGCCGGAAACTCTGACGAACTACTGGGGCGACCAACCGGAGGAAGAATTCTACGCCTCACAAGGAGTTCGAAACTCCAAATCCTTCTTCGAAACCCCCCATGGCAAGCTCTTCACACAGAGCTTCATTCCTCTGGACTGGCCAGAGCCCAAAGGCACCGTCTACATGACTCACGGCTACGGCTCCGACACCGGCTGGATGTTCCAAAAGATCTGTCTCAGCTATGCCTCTTGGGGCTACGCCGTCTTCGCCGCCGATCTCCTTGGCCATGGCCGCTCCGATGGCCTCCGCTGCTACCTCGGCGATATGGAAAAAGTCGCCGCCGCCTCCCTCTCTTTCTTCCTCCACACCCGCCGCAGCGAGCCCTACTGCCATCTCCCTGCCTTCCTCTTCGGCGAGTCCATGGGCGGTGCCGCCACCATGCTCATGTACTTCCAGTCAGATCCAGACACCTGGACCGGTCTGATCTTCTCGGCGCCGCTTTTTGTCATTCCGGAAAACATGAAGCCGAGCAAACTCCGCCTCTTCCTCTACGGCCTCCTCTTCGGCGTCGCCGACACTTGGGCCGCCATGCCGGACAATAAAATGGTGGGAAAGGCGATTAAGGATCCGGAGAAGTTGAAAATCATAGCGGCGAATCCTAGACGGTACACAGGACCACCGAGGGTTGGGACAATGAGGGAATTGGTTAGAGTTACGCAGTACATTAGAGACAATTTTTCGAGGGTTACGGCGCCATTTCTGACGGTGCACGGGACGGCGGACGGAGTGACGTGTCCGTCGTCGTCGGAATTACTGTACGAGAAGGCGGCGAGCGTGGATAAGACCTTGAAGCTTTACGAGGGGATGTACCATTCGTTAATTCAAGGGGAGCCGGATGAGAATATGGAAATTGTTCTGAGGGATATGAGGGAGTGGATCGACGAGAGGGTTCAGAGATATGGATCGAAGAAATAG

Protein sequence

MAPEPPAPETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLDWPEPKGTVYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFFLHTRRSEPYCHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLRLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDENMEIVLRDMREWIDERVQRYGSKK

Homology

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BLAST of CmaCh01G001250 vs. ExPASy Swiss-Prot
Match: Q9C942 (Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 2.8e-153
Identity = 252/319 (79.00%), Postives = 286/319 (89.66%), Query Frame = 0


Query: 5   PPAPETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLDWPEPKGTVYMTH 64
           P  P    N+WG  PEEE+Y SQGVRNSKS+FETP+GKLFTQSF+PLD  E KGTVYM+H
Sbjct: 13  PATPPPPPNFWGTMPEEEYYTSQGVRNSKSYFETPNGKLFTQSFLPLD-GEIKGTVYMSH 72

Query: 65  GYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFFLHTR 124
           GYGSDT WMFQKIC+S++SWGYAVFAADLLGHGRSDG+RCY+GDMEKVAA SL+FF H R
Sbjct: 73  GYGSDTSWMFQKICMSFSSWGYAVFAADLLGHGRSDGIRCYMGDMEKVAATSLAFFKHVR 132

Query: 125 RSEPYCHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLRLFLY 184
            S+PY  LPAFLFGESMGG  T+LMYFQS+P+TWTGL+FSAPLFVIPE+MKPSK  LF Y
Sbjct: 133 CSDPYKDLPAFLFGESMGGLVTLLMYFQSEPETWTGLMFSAPLFVIPEDMKPSKAHLFAY 192

Query: 185 GLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRD 244
           GLLFG+ADTWAAMPDNKMVGKAIKDPEKLKIIA+NP+RYTG PRVGTMREL+R TQY+++
Sbjct: 193 GLLFGLADTWAAMPDNKMVGKAIKDPEKLKIIASNPQRYTGKPRVGTMRELLRKTQYVQE 252

Query: 245 NFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDENMEI 304
           NF +VT P  T HGTADGVTCP+SS+LLYEKA+S DKTLK+YEGMYHSLIQGEPDEN EI
Sbjct: 253 NFGKVTIPVFTAHGTADGVTCPTSSKLLYEKASSADKTLKIYEGMYHSLIQGEPDENAEI 312

Query: 305 VLRDMREWIDERVQRYGSK 324
           VL+DMREWIDE+V++YGSK
Sbjct: 313 VLKDMREWIDEKVKKYGSK 330

[Close]

BLAST of CmaCh01G001250 vs. ExPASy Swiss-Prot
Match: A0QNZ7 (Monoacylglycerol lipase OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) OX=246196 GN=MSMEG_0220 PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 2.3e-22
Identity = 73/260 (28.08%), Postives = 119/260 (45.77%), Query Frame = 0


Query: 55  EPKGTVYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAA 114
           +P+G V + HGY    G  +  +   + + G  V+A D  GHGRS G R +L D+ +   
Sbjct: 29  DPRGVVVLAHGYAEHAG-RYHHVAQRFGAAGLLVYALDHRGHGRSGGKRVHLRDLSEFVE 88

Query: 115 ASLSFFLHTRRSEPYCHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENM 174
              +         P   LP  + G SMGG   +  Y    P  ++ ++ S P     + +
Sbjct: 89  DFRTLVGIAANDHP--TLPRIVLGHSMGG-GIVFAYGARYPGEYSAMVLSGPAVNAHDGV 148

Query: 175 KPSKLRLFLYGLLFGVADTWAAMPDNKMVGKAI-KDPEKLKIIAANPRRYTGPPRVGTMR 234
            P      L  +   +      +P   +   A+ +DPE +    A+P  + G    G  R
Sbjct: 149 SP-----VLVAVAKVLGKLAPGIPVENLDADAVSRDPEVVAAYKADPMVHHGKLPAGIAR 208

Query: 235 ELVRVTQYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSL 294
            L+ + Q +    + +TAP L VHG  D +   + S LL ++ AS D  LK+Y G+YH +
Sbjct: 209 ALIGLGQSMPQRAAALTAPLLVVHGDKDRLIPVAGSRLLVDRVASEDVHLKVYPGLYHEV 268

Query: 295 IQGEPDENMEIVLRDMREWI 314
              EP++  ++VL D+  WI
Sbjct: 269 F-NEPEQ--KLVLDDVTSWI 276

[Close]

BLAST of CmaCh01G001250 vs. ExPASy Swiss-Prot
Match: Q8R431 (Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 2.8e-20
Identity = 74/269 (27.51%), Postives = 133/269 (49.44%), Query Frame = 0


Query: 56  PKGTVYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAA 115
           PK  ++++HG G   G  + ++          VFA D +GHG+S+G R  + D + V   
Sbjct: 41  PKALIFVSHGAGEHCG-RYDELAQMLKRLDMLVFAHDHVGHGQSEGERMVVSDFQ-VFVR 100

Query: 116 SLSFFLHTRRSEPYCHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMK 175
            L   ++T + + Y  +P FL G SMGGA ++L   +  P  ++G+I  +PL ++     
Sbjct: 101 DLLQHVNTVQKD-YPEVPVFLLGHSMGGAISILAAAER-PTHFSGMILISPL-ILANPES 160

Query: 176 PSKLRLFLYGLL-FGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRE 235
            S L++    LL F + +      D+ ++    ++  ++ +  ++P       +V    +
Sbjct: 161 ASTLKVLAAKLLNFVLPNISLGRIDSSVLS---RNKSEVDLYNSDPLICHAGVKVCFGIQ 220

Query: 236 LVRVTQYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLI 295
           L+     +     R+T PFL + G+AD +     + LL E + S DKTLK+YEG YH ++
Sbjct: 221 LLNAVSRVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSPSQDKTLKMYEGAYH-VL 280

Query: 296 QGEPDENMEIVLRDMREWIDERVQRYGSK 324
             E  E    VL ++  W+  R+   G++
Sbjct: 281 HKELPEVTNSVLHEINTWVSHRIAVAGAR 300

[Close]

BLAST of CmaCh01G001250 vs. ExPASy Swiss-Prot
Match: O35678 (Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 3.6e-20
Identity = 72/268 (26.87%), Postives = 129/268 (48.13%), Query Frame = 0


Query: 56  PKGTVYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAA 115
           PK  ++++HG G   G  + ++          VFA D +GHG+S+G R  + D +     
Sbjct: 41  PKALIFVSHGAGEHCG-RYDELAHMLKGLDMLVFAHDHVGHGQSEGERMVVSDFQVFVRD 100

Query: 116 SLSFFLHTRRSEPYCHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMK 175
            L      ++  P   +P FL G SMGGA ++L+  +  P  ++G++  +PL V+     
Sbjct: 101 VLQHVDTIQKDYP--DVPIFLLGHSMGGAISILVAAER-PTYFSGMVLISPL-VLANPES 160

Query: 176 PSKLRLFLYGLL-FGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRE 235
            S L++    LL F + +      D+ ++    ++  ++ +  ++P       +V    +
Sbjct: 161 ASTLKVLAAKLLNFVLPNMTLGRIDSSVLS---RNKSEVDLYNSDPLVCRAGLKVCFGIQ 220

Query: 236 LVRVTQYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLI 295
           L+     +     R+T PFL + G+AD +     + LL E + S DKTLK+YEG YH ++
Sbjct: 221 LLNAVARVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSRSQDKTLKMYEGAYH-VL 280

Query: 296 QGEPDENMEIVLRDMREWIDERVQRYGS 323
             E  E    VL ++  W+  R+   G+
Sbjct: 281 HRELPEVTNSVLHEVNSWVSHRIAAAGA 299

[Close]

BLAST of CmaCh01G001250 vs. ExPASy Swiss-Prot
Match: Q99685 (Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2)

HSP 1 Score: 99.0 bits (245), Expect = 1.1e-19
Identity = 77/274 (28.10%), Postives = 125/274 (45.62%), Query Frame = 0


Query: 56  PKGTVYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAA 115
           PK  ++++HG G  +G  ++++          VFA D +GHG+S+G R  + D       
Sbjct: 41  PKALIFVSHGAGEHSG-RYEELARMLMGLDLLVFAHDHVGHGQSEGERMVVSDFHVFVRD 100

Query: 116 SLSFFLHTRRSEPYCHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVI-PENM 175
            L      ++  P   LP FL G SMGGA  +L   +  P  + G++  +PL +  PE+ 
Sbjct: 101 VLQHVDSMQKDYP--GLPVFLLGHSMGGAIAILTAAER-PGHFAGMVLISPLVLANPESA 160

Query: 176 KPSK------LRLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPR 235
              K      L L L  L  G  D+ + +  NK          ++ I  ++P       +
Sbjct: 161 TTFKVLAAKVLNLVLPNLSLGPIDS-SVLSRNK---------TEVDIYNSDPLICRAGLK 220

Query: 236 VGTMRELVRVTQYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEG 295
           V    +L+     +     ++T PFL + G+AD +     + LL E A S DKTLK+YEG
Sbjct: 221 VCFGIQLLNAVSRVERALPKLTVPFLLLQGSADRLCDSKGAYLLMELAKSQDKTLKIYEG 280

Query: 296 MYHSLIQGEPDENMEIVLRDMREWIDERVQRYGS 323
            YH ++  E  E    V  ++  W+ +R    G+
Sbjct: 281 AYH-VLHKELPEVTNSVFHEINMWVSQRTATAGT 299

[Close]

BLAST of CmaCh01G001250 vs. ExPASy TrEMBL
Match: A0A6J1K763 (caffeoylshikimate esterase OS=Cucurbita maxima OX=3661 GN=LOC111492898 PE=4 SV=1)

HSP 1 Score: 678.7 bits (1750), Expect = 1.2e-191
Identity = 324/324 (100.00%), Postives = 324/324 (100.00%), Query Frame = 0


Query: 1   MAPEPPAPETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLDWPEPKGTV 60
           MAPEPPAPETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLDWPEPKGTV
Sbjct: 1   MAPEPPAPETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLDWPEPKGTV 60

Query: 61  YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFF 120
           YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFF
Sbjct: 61  YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFF 120

Query: 121 LHTRRSEPYCHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR 180
           LHTRRSEPYCHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR
Sbjct: 121 LHTRRSEPYCHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR 180

Query: 181 LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ 240
           LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ
Sbjct: 181 LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ 240

Query: 241 YIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDE 300
           YIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDE
Sbjct: 241 YIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDE 300

Query: 301 NMEIVLRDMREWIDERVQRYGSKK 325
           NMEIVLRDMREWIDERVQRYGSKK
Sbjct: 301 NMEIVLRDMREWIDERVQRYGSKK 324

[Close]

BLAST of CmaCh01G001250 vs. ExPASy TrEMBL
Match: A0A6J1GBG1 (caffeoylshikimate esterase OS=Cucurbita moschata OX=3662 GN=LOC111452643 PE=4 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 1.0e-187
Identity = 321/324 (99.07%), Postives = 321/324 (99.07%), Query Frame = 0


Query: 1   MAPEPPAPETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLDWPEPKGTV 60
           MAPEP APETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLD PEPKGTV
Sbjct: 1   MAPEPAAPETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLDSPEPKGTV 60

Query: 61  YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFF 120
           YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFF
Sbjct: 61  YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFF 120

Query: 121 LHTRRSEPYCHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR 180
           LHTRRSEPY HLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR
Sbjct: 121 LHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR 180

Query: 181 LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ 240
           LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ
Sbjct: 181 LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ 240

Query: 241 YIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDE 300
           YIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDE
Sbjct: 241 YIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDE 300

Query: 301 NMEIVLRDMREWIDERVQRYGSKK 325
           NMEIVLRDMREWIDERVQRYGSKK
Sbjct: 301 NMEIVLRDMREWIDERVQRYGSKK 324

[Close]

BLAST of CmaCh01G001250 vs. ExPASy TrEMBL
Match: A0A5D3DA65 (Caffeoylshikimate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00400 PE=4 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 2.4e-176
Identity = 302/324 (93.21%), Postives = 311/324 (95.99%), Query Frame = 0


Query: 1   MAPEPPAPETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLDWPEPKGTV 60
           M PEPP      N+WGDQPEEEFYASQGVRN+KSFFETPHGKLFTQSFIPLD P+ KGTV
Sbjct: 1   MPPEPP-----LNFWGDQPEEEFYASQGVRNTKSFFETPHGKLFTQSFIPLDSPDLKGTV 60

Query: 61  YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFF 120
           YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDM+K+AAASLSFF
Sbjct: 61  YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSFF 120

Query: 121 LHTRRSEPYCHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR 180
           LHTRRSEPY HLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR
Sbjct: 121 LHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR 180

Query: 181 LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ 240
           LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ
Sbjct: 181 LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ 240

Query: 241 YIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDE 300
           YIRDNFS+VTAPFLTVHGTADGVTCPSSS+LLYEKAASVDKTLKLY+GMYHSLIQGEPDE
Sbjct: 241 YIRDNFSKVTAPFLTVHGTADGVTCPSSSQLLYEKAASVDKTLKLYDGMYHSLIQGEPDE 300

Query: 301 NMEIVLRDMREWIDERVQRYGSKK 325
           N+EIVLRDMREWIDER  RYG KK
Sbjct: 301 NVEIVLRDMREWIDERALRYGPKK 319

[Close]

BLAST of CmaCh01G001250 vs. ExPASy TrEMBL
Match: A0A1S3BHJ4 (caffeoylshikimate esterase OS=Cucumis melo OX=3656 GN=LOC103489918 PE=4 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 2.4e-176
Identity = 302/324 (93.21%), Postives = 311/324 (95.99%), Query Frame = 0


Query: 1   MAPEPPAPETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLDWPEPKGTV 60
           M PEPP      N+WGDQPEEEFYASQGVRN+KSFFETPHGKLFTQSFIPLD P+ KGTV
Sbjct: 1   MPPEPP-----LNFWGDQPEEEFYASQGVRNTKSFFETPHGKLFTQSFIPLDSPDLKGTV 60

Query: 61  YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFF 120
           YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDM+K+AAASLSFF
Sbjct: 61  YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSFF 120

Query: 121 LHTRRSEPYCHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR 180
           LHTRRSEPY HLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR
Sbjct: 121 LHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR 180

Query: 181 LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ 240
           LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ
Sbjct: 181 LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ 240

Query: 241 YIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDE 300
           YIRDNFS+VTAPFLTVHGTADGVTCPSSS+LLYEKAASVDKTLKLY+GMYHSLIQGEPDE
Sbjct: 241 YIRDNFSKVTAPFLTVHGTADGVTCPSSSQLLYEKAASVDKTLKLYDGMYHSLIQGEPDE 300

Query: 301 NMEIVLRDMREWIDERVQRYGSKK 325
           N+EIVLRDMREWIDER  RYG KK
Sbjct: 301 NVEIVLRDMREWIDERALRYGPKK 319

[Close]

BLAST of CmaCh01G001250 vs. ExPASy TrEMBL
Match: A0A0A0LBT8 (Hydrolase_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G539670 PE=4 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 2.9e-174
Identity = 297/319 (93.10%), Postives = 306/319 (95.92%), Query Frame = 0


Query: 6   PAPETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLDWPEPKGTVYMTHG 65
           P PE   N+WGDQPEEEFYASQGVRN+KSFFET HGKLFTQSFIPLD+P+ KGTVYMTHG
Sbjct: 7   PPPEPPLNFWGDQPEEEFYASQGVRNTKSFFETSHGKLFTQSFIPLDFPDLKGTVYMTHG 66

Query: 66  YGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFFLHTRR 125
           YGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDM+K+AAASLSFFLHTRR
Sbjct: 67  YGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSFFLHTRR 126

Query: 126 SEPYCHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLRLFLYG 185
           SEPY HLPAFLFGESMG AATMLMY QSDPDTWTGLIFSAPLFVIPENMKPSKLRLFLYG
Sbjct: 127 SEPYRHLPAFLFGESMGCAATMLMYLQSDPDTWTGLIFSAPLFVIPENMKPSKLRLFLYG 186

Query: 186 LLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDN 245
           LLFGVADTWAAMPDNKMVGKAIKDP+KLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDN
Sbjct: 187 LLFGVADTWAAMPDNKMVGKAIKDPQKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDN 246

Query: 246 FSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDENMEIV 305
           FSRVTAPFLTVHGTADGVTCPSSSELLYEKA SVDKTLKLY+GMYHSLIQGEPDEN+EIV
Sbjct: 247 FSRVTAPFLTVHGTADGVTCPSSSELLYEKATSVDKTLKLYDGMYHSLIQGEPDENVEIV 306

Query: 306 LRDMREWIDERVQRYGSKK 325
           LRDMREWIDER  RYG KK
Sbjct: 307 LRDMREWIDERALRYGPKK 325

[Close]

BLAST of CmaCh01G001250 vs. NCBI nr
Match: XP_022998172.1 (caffeoylshikimate esterase [Cucurbita maxima])

HSP 1 Score: 678.7 bits (1750), Expect = 2.5e-191
Identity = 324/324 (100.00%), Postives = 324/324 (100.00%), Query Frame = 0


Query: 1   MAPEPPAPETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLDWPEPKGTV 60
           MAPEPPAPETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLDWPEPKGTV
Sbjct: 1   MAPEPPAPETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLDWPEPKGTV 60

Query: 61  YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFF 120
           YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFF
Sbjct: 61  YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFF 120

Query: 121 LHTRRSEPYCHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR 180
           LHTRRSEPYCHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR
Sbjct: 121 LHTRRSEPYCHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR 180

Query: 181 LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ 240
           LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ
Sbjct: 181 LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ 240

Query: 241 YIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDE 300
           YIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDE
Sbjct: 241 YIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDE 300

Query: 301 NMEIVLRDMREWIDERVQRYGSKK 325
           NMEIVLRDMREWIDERVQRYGSKK
Sbjct: 301 NMEIVLRDMREWIDERVQRYGSKK 324

[Close]

BLAST of CmaCh01G001250 vs. NCBI nr
Match: XP_023525534.1 (caffeoylshikimate esterase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 667.2 bits (1720), Expect = 7.4e-188
Identity = 321/324 (99.07%), Postives = 322/324 (99.38%), Query Frame = 0


Query: 1   MAPEPPAPETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLDWPEPKGTV 60
           MAPEPPAPETLTNYWGDQPEEEFYASQGV+NSKSFFETPHGKLFTQSFIPLD PEPKGTV
Sbjct: 1   MAPEPPAPETLTNYWGDQPEEEFYASQGVQNSKSFFETPHGKLFTQSFIPLDSPEPKGTV 60

Query: 61  YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFF 120
           YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFF
Sbjct: 61  YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFF 120

Query: 121 LHTRRSEPYCHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR 180
           LHTRRSEPY HLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR
Sbjct: 121 LHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR 180

Query: 181 LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ 240
           LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ
Sbjct: 181 LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ 240

Query: 241 YIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDE 300
           YIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDE
Sbjct: 241 YIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDE 300

Query: 301 NMEIVLRDMREWIDERVQRYGSKK 325
           NMEIVLRDMREWIDERVQRYGSKK
Sbjct: 301 NMEIVLRDMREWIDERVQRYGSKK 324

[Close]

BLAST of CmaCh01G001250 vs. NCBI nr
Match: KAG6606779.1 (Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036492.1 Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 666.8 bits (1719), Expect = 9.7e-188
Identity = 320/324 (98.77%), Postives = 322/324 (99.38%), Query Frame = 0


Query: 1   MAPEPPAPETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLDWPEPKGTV 60
           MAPEPPAPETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLD PEPKGTV
Sbjct: 1   MAPEPPAPETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLDSPEPKGTV 60

Query: 61  YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFF 120
           YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFF
Sbjct: 61  YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFF 120

Query: 121 LHTRRSEPYCHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR 180
           LHTRRSEPY HLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR
Sbjct: 121 LHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR 180

Query: 181 LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ 240
           LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ
Sbjct: 181 LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ 240

Query: 241 YIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDE 300
           YIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKT+KLYEGMYHSLIQGEPDE
Sbjct: 241 YIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTMKLYEGMYHSLIQGEPDE 300

Query: 301 NMEIVLRDMREWIDERVQRYGSKK 325
           NMEIVLRDMREWIDERVQRYGSK+
Sbjct: 301 NMEIVLRDMREWIDERVQRYGSKR 324

[Close]

BLAST of CmaCh01G001250 vs. NCBI nr
Match: XP_022949228.1 (caffeoylshikimate esterase [Cucurbita moschata])

HSP 1 Score: 665.6 bits (1716), Expect = 2.2e-187
Identity = 321/324 (99.07%), Postives = 321/324 (99.07%), Query Frame = 0


Query: 1   MAPEPPAPETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLDWPEPKGTV 60
           MAPEP APETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLD PEPKGTV
Sbjct: 1   MAPEPAAPETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLDSPEPKGTV 60

Query: 61  YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFF 120
           YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFF
Sbjct: 61  YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFF 120

Query: 121 LHTRRSEPYCHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR 180
           LHTRRSEPY HLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR
Sbjct: 121 LHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR 180

Query: 181 LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ 240
           LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ
Sbjct: 181 LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ 240

Query: 241 YIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDE 300
           YIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDE
Sbjct: 241 YIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDE 300

Query: 301 NMEIVLRDMREWIDERVQRYGSKK 325
           NMEIVLRDMREWIDERVQRYGSKK
Sbjct: 301 NMEIVLRDMREWIDERVQRYGSKK 324

[Close]

BLAST of CmaCh01G001250 vs. NCBI nr
Match: XP_008447481.1 (PREDICTED: caffeoylshikimate esterase [Cucumis melo] >KAA0038136.1 caffeoylshikimate esterase [Cucumis melo var. makuwa] >TYK20471.1 caffeoylshikimate esterase [Cucumis melo var. makuwa])

HSP 1 Score: 627.9 bits (1618), Expect = 5.0e-176
Identity = 302/324 (93.21%), Postives = 311/324 (95.99%), Query Frame = 0


Query: 1   MAPEPPAPETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLDWPEPKGTV 60
           M PEPP      N+WGDQPEEEFYASQGVRN+KSFFETPHGKLFTQSFIPLD P+ KGTV
Sbjct: 1   MPPEPP-----LNFWGDQPEEEFYASQGVRNTKSFFETPHGKLFTQSFIPLDSPDLKGTV 60

Query: 61  YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFF 120
           YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDM+K+AAASLSFF
Sbjct: 61  YMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSFF 120

Query: 121 LHTRRSEPYCHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR 180
           LHTRRSEPY HLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR
Sbjct: 121 LHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLR 180

Query: 181 LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ 240
           LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ
Sbjct: 181 LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQ 240

Query: 241 YIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDE 300
           YIRDNFS+VTAPFLTVHGTADGVTCPSSS+LLYEKAASVDKTLKLY+GMYHSLIQGEPDE
Sbjct: 241 YIRDNFSKVTAPFLTVHGTADGVTCPSSSQLLYEKAASVDKTLKLYDGMYHSLIQGEPDE 300

Query: 301 NMEIVLRDMREWIDERVQRYGSKK 325
           N+EIVLRDMREWIDER  RYG KK
Sbjct: 301 NVEIVLRDMREWIDERALRYGPKK 319

[Close]

BLAST of CmaCh01G001250 vs. TAIR 10
Match: AT1G52760.1 (lysophospholipase 2 )

HSP 1 Score: 542.7 bits (1397), Expect = 2.0e-154
Identity = 252/319 (79.00%), Postives = 286/319 (89.66%), Query Frame = 0


Query: 5   PPAPETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLDWPEPKGTVYMTH 64
           P  P    N+WG  PEEE+Y SQGVRNSKS+FETP+GKLFTQSF+PLD  E KGTVYM+H
Sbjct: 13  PATPPPPPNFWGTMPEEEYYTSQGVRNSKSYFETPNGKLFTQSFLPLD-GEIKGTVYMSH 72

Query: 65  GYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFFLHTR 124
           GYGSDT WMFQKIC+S++SWGYAVFAADLLGHGRSDG+RCY+GDMEKVAA SL+FF H R
Sbjct: 73  GYGSDTSWMFQKICMSFSSWGYAVFAADLLGHGRSDGIRCYMGDMEKVAATSLAFFKHVR 132

Query: 125 RSEPYCHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLRLFLY 184
            S+PY  LPAFLFGESMGG  T+LMYFQS+P+TWTGL+FSAPLFVIPE+MKPSK  LF Y
Sbjct: 133 CSDPYKDLPAFLFGESMGGLVTLLMYFQSEPETWTGLMFSAPLFVIPEDMKPSKAHLFAY 192

Query: 185 GLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRD 244
           GLLFG+ADTWAAMPDNKMVGKAIKDPEKLKIIA+NP+RYTG PRVGTMREL+R TQY+++
Sbjct: 193 GLLFGLADTWAAMPDNKMVGKAIKDPEKLKIIASNPQRYTGKPRVGTMRELLRKTQYVQE 252

Query: 245 NFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDENMEI 304
           NF +VT P  T HGTADGVTCP+SS+LLYEKA+S DKTLK+YEGMYHSLIQGEPDEN EI
Sbjct: 253 NFGKVTIPVFTAHGTADGVTCPTSSKLLYEKASSADKTLKIYEGMYHSLIQGEPDENAEI 312

Query: 305 VLRDMREWIDERVQRYGSK 324
           VL+DMREWIDE+V++YGSK
Sbjct: 313 VLKDMREWIDEKVKKYGSK 330

[Close]

BLAST of CmaCh01G001250 vs. TAIR 10
Match: AT1G11090.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 267.7 bits (683), Expect = 1.2e-71
Identity = 135/310 (43.55%), Postives = 196/310 (63.23%), Query Frame = 0


Query: 9   ETLTNYWGDQPEEEFYASQGVRNSKSFFETPHG-KLFTQSFIPLDWPEPKGTVYMTHGYG 68
           ET  N+WG+  EE+++  +G+  SKSFF +P G  LFT+S++P     P+G ++M HGYG
Sbjct: 7   ETEINFWGETSEEDYFNLKGIIGSKSFFTSPRGLNLFTRSWLPSSSSPPRGLIFMVHGYG 66

Query: 69  SDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFFLHTRRSE 128
           +D  W FQ   +  A  G+A FA D+ GHGRSDG+R Y+  ++ V    +SFF   +++ 
Sbjct: 67  NDVSWTFQSTPIFLAQMGFACFALDIEGHGRSDGVRAYVPSVDLVVDDIISFFNSIKQNP 126

Query: 129 PYCHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPS-KLRLFLYGL 188
            +  LP FLFGESMGGA  +L+ F +DP  + G +  AP+  I + ++P   +  FL  +
Sbjct: 127 KFQGLPRFLFGESMGGAICLLIQF-ADPLGFDGAVLVAPMCKISDKVRPKWPVDQFLI-M 186

Query: 189 LFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNF 248
           +     TWA +P   ++ K+IK  EK  I   NP RY   PR+GT+ EL+RVT Y+    
Sbjct: 187 ISRFLPTWAIVPTEDLLEKSIKVEEKKPIAKRNPMRYNEKPRLGTVMELLRVTDYLGKKL 246

Query: 249 SRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDENMEIVL 308
             V+ PF+ VHG+AD VT P  S  LYE A S DKTLK+Y+GM HS++ GEPD+N+EIV 
Sbjct: 247 KDVSIPFIIVHGSADAVTDPEVSRELYEHAKSKDKTLKIYDGMMHSMLFGEPDDNIEIVR 306

Query: 309 RDMREWIDER 317
           +D+  W+++R
Sbjct: 307 KDIVSWLNDR 314

[Close]

BLAST of CmaCh01G001250 vs. TAIR 10
Match: AT2G47630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 203.8 bits (517), Expect = 2.2e-52
Identity = 110/293 (37.54%), Postives = 156/293 (53.24%), Query Frame = 0


Query: 24  YASQGVRNSKSFFETPHGKLFTQSFIPLDWPEPKGTVYMTHGYGSDTGWMFQKICLSYAS 83
           Y  + VRNS+        +LF   +IP     PK  V++ HGYG +     ++  +  AS
Sbjct: 8   YHEEYVRNSRGV------ELFACRWIPSSSSSPKALVFLCHGYGMECSDSMKECGIRLAS 67

Query: 84  WGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFFLHTRRSEPYCHLPAFLFGESMGG 143
            GYAVF  D  GHGRS G RCY+     V      ++      E Y     FL+GESMGG
Sbjct: 68  AGYAVFGMDYEGHGRSMGSRCYIKKFANVVNDCYDYYTSICAQEEYMDKGRFLYGESMGG 127

Query: 144 AATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLRLFLYGLLFGVADTWAAMPDNKMV 203
           A T+L++ + DP  W G I  AP+  I E +KP  + + L   +  +   W  +P   ++
Sbjct: 128 AVTLLLH-KKDPLFWNGAILVAPMCKISEKVKPHPIVINLLTRVEEIIPKWKIVPTKDVI 187

Query: 204 GKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNFSRVTAPFLTVHGTADGV 263
             A KD  K + +  N   Y   PR+ T  E++R +  + D    +T PF  +HG AD V
Sbjct: 188 DAAFKDLVKREEVRNNKLIYQDKPRLKTALEMLRTSMNLEDTLHEITMPFFVLHGEADTV 247

Query: 264 TCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDENMEIVLRDMREWIDER 317
           T P  S+ LYEKA++ DKTLKLY GM+H+L  GEPD N+++V  D+  W+D R
Sbjct: 248 TDPEVSKALYEKASTRDKTLKLYPGMWHALTSGEPDCNVDLVFADIINWLDLR 293

[Close]

BLAST of CmaCh01G001250 vs. TAIR 10
Match: AT3G62860.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 201.1 bits (510), Expect = 1.4e-51
Identity = 103/293 (35.15%), Postives = 155/293 (52.90%), Query Frame = 0


Query: 24  YASQGVRNSKSFFETPHGKLFTQSFIPLDWPEPKGTVYMTHGYGSDTGWMFQKICLSYAS 83
           Y  + ++NS+        +LF   ++P     P+  V++ HGYG +     ++  +  AS
Sbjct: 8   YEEEYIKNSRDV------ELFACRWLPSS--SPRALVFLCHGYGMECSSFMRECGIRLAS 67

Query: 84  WGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSFFLHTRRSEPYCHLPAFLFGESMGG 143
            GYAVF  D  GHGRS G RCY+     +      ++      E Y     FL+GESMGG
Sbjct: 68  AGYAVFGMDYEGHGRSKGARCYIKKFSNIVNDCFDYYTSISAQEEYKEKGRFLYGESMGG 127

Query: 144 AATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLRLFLYGLLFGVADTWAAMPDNKMV 203
           A  +L++ + DP  W G +  AP+  I E +KP  + + L   +  +   W  +P   ++
Sbjct: 128 AVALLLH-KKDPSFWNGALLVAPMCKISEKVKPHPVVINLLTRVEDIIPKWKIVPTKDVI 187

Query: 204 GKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNFSRVTAPFLTVHGTADGV 263
             A KDP K + I  N   Y   PR+ T  E++R +  + D    +T PF  +HG AD V
Sbjct: 188 DAAFKDPVKREEIRNNKLIYQDKPRLKTALEMLRTSMDLEDTLHEITLPFFVLHGEADIV 247

Query: 264 TCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPDENMEIVLRDMREWIDER 317
           T P  S+ L+EKA++ DKT+KLY GM+H L  GEPD N+++V  D+  W+D R
Sbjct: 248 TDPEISKALFEKASTRDKTIKLYPGMWHGLTSGEPDANVDLVFADIVNWLDAR 291

[Close]

BLAST of CmaCh01G001250 vs. TAIR 10
Match: AT5G14980.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 198.0 bits (502), Expect = 1.2e-50
Identity = 114/318 (35.85%), Postives = 167/318 (52.52%), Query Frame = 0


Query: 1   MAPEPPAPETLTNYWGDQPEEEFYASQGVRNSKSFFETPHG-KLFTQSFIPLDWPEPKGT 60
           M   P +    ++ +G     EFY+   V +S ++   P G KLFTQ + PL+ P P G 
Sbjct: 1   MGLHPVSEANASSPFGSLSAAEFYSRHSVTHSSAYVTNPTGLKLFTQWWTPLNRP-PLGL 60

Query: 61  VYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSF 120
           + + HG+  ++ W  Q   + +A  GY   A D  GHG SDGL  ++ ++  +    +SF
Sbjct: 61  IAVVHGFTGESSWFLQLTSVLFAKSGYLTCAIDHQGHGFSDGLTAHIPNINLIVDDCISF 120

Query: 121 FLHTRRSEPYCHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKL 180
           F   R+      LPAFL+ ES+GGA  + +  +     W GLI S  +  I    KP   
Sbjct: 121 FDDFRKRHASSFLPAFLYSESLGGAIALYITLR-QKHQWNGLILSGAMCSISHKFKPPWP 180

Query: 181 RLFLYGLLFGVADTWAAMPD-NKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRV 240
              L  L   +  TW  +P    + G + K+P K K+  ANP R  G PR  T  ELVRV
Sbjct: 181 LQHLLTLAATLIPTWRVVPTRGSIAGVSFKEPWKRKLAYANPNRTVGKPRAATAYELVRV 240

Query: 241 TQYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEP 300
            + +++ F  V  P + VHG  D V  P+S E LY + +S DKT+K+Y GM+H LI GE 
Sbjct: 241 CEDLQNRFEEVEVPLMIVHGRDDVVCDPASVEELYRRCSSRDKTIKIYPGMWHQLI-GES 300

Query: 301 DENMEIVLRDMREWIDER 317
           +EN+++V  D+ +WI  R
Sbjct: 301 EENVDLVFGDVLDWIKTR 315

The following BLAST results are available for this feature:

BLAST of CmaCh01G001250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C942	2.8e-153	79.00	Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1	[more]
A0QNZ7	2.3e-22	28.08	Monoacylglycerol lipase OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(...	[more]
Q8R431	2.8e-20	27.51	Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1	[more]
O35678	3.6e-20	26.87	Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1	[more]
Q99685	1.1e-19	28.10	Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2	[more]

BLAST of CmaCh01G001250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K763	1.2e-191	100.00	caffeoylshikimate esterase OS=Cucurbita maxima OX=3661 GN=LOC111492898 PE=4 SV=1	[more]
A0A6J1GBG1	1.0e-187	99.07	caffeoylshikimate esterase OS=Cucurbita moschata OX=3662 GN=LOC111452643 PE=4 SV...	[more]
A0A5D3DA65	2.4e-176	93.21	Caffeoylshikimate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A1S3BHJ4	2.4e-176	93.21	caffeoylshikimate esterase OS=Cucumis melo OX=3656 GN=LOC103489918 PE=4 SV=1	[more]
A0A0A0LBT8	2.9e-174	93.10	Hydrolase_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G539670...	[more]

BLAST of CmaCh01G001250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022998172.1	2.5e-191	100.00	caffeoylshikimate esterase [Cucurbita maxima]	[more]
XP_023525534.1	7.4e-188	99.07	caffeoylshikimate esterase [Cucurbita pepo subsp. pepo]	[more]
KAG6606779.1	9.7e-188	98.77	Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. sororia] >KAG...	[more]
XP_022949228.1	2.2e-187	99.07	caffeoylshikimate esterase [Cucurbita moschata]	[more]
XP_008447481.1	5.0e-176	93.21	PREDICTED: caffeoylshikimate esterase [Cucumis melo] >KAA0038136.1 caffeoylshiki...	[more]

BLAST of CmaCh01G001250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52760.1	2.0e-154	79.00	lysophospholipase 2	[more]
AT1G11090.1	1.2e-71	43.55	alpha/beta-Hydrolases superfamily protein	[more]
AT2G47630.1	2.2e-52	37.54	alpha/beta-Hydrolases superfamily protein	[more]
AT3G62860.1	1.4e-51	35.15	alpha/beta-Hydrolases superfamily protein	[more]
AT5G14980.1	1.2e-50	35.85	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000073	Alpha/beta hydrolase fold-1	PRINTS	PR00111	ABHYDROLASE	coord: 85..100 score: 41.81 coord: 136..149 score: 41.63
IPR022742	Serine aminopeptidase, S33	PFAM	PF12146	Hydrolase_4	coord: 55..298 e-value: 1.5E-50 score: 171.7
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 25..323 e-value: 5.0E-50 score: 172.4
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 22..317
None	No IPR available	PANTHER	PTHR11614:SF163	CAFFEOYLSHIKIMATE ESTERASE	coord: 15..322
None	No IPR available	PANTHER	PTHR11614	PHOSPHOLIPASE-RELATED	coord: 15..322

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh01G001250.1	CmaCh01G001250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0016298	lipase activity