CmaCh01G001140 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh01G001140
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionnitrate regulatory gene2 protein-like
LocationCma_Chr01: 517164 .. 520209 (-)
RNA-Seq ExpressionCmaCh01G001140
SyntenyCmaCh01G001140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCCCAGCTTTTTTTTTTTTTTTTTTCTTTTTTTCTGTTTGGGTTCTCAAATCCTTCATGCTCAAGTCTTGATTCCGCCATCGCCGCCGATTAGCTGAGCTTAATGGGTTGTTCTCAGTCGAAGATCGATAATGAAGAAGCGATAGCCCGTTGTAAAGAACGGAAGATTCATATGAAGGAAGCGGCGACGGCTCGAAACGCTTTCGCCGCTGCGCACTCCGCCTATTCTATGTCGCTGAAAAATATTGGAGCTGCTTTAAGTGATTATGCCCATGGTGAGGTTGAAAATTCCCAATTTGTTGATGTATCTACTCAACCTAATTCTGCCATTACTTCCGCTCCCGCCCCTATTGAACCTTTTCCGCCGCCGCCGCCTTTGCCTCCTTCTAATTTTCCCAATTCTCTTGAAAGGGCGGCCACCATGCCTGAGATGAATGTGCACAAATCCGATCTCAAGCCAGGATCGCCGATTATTGAGGAAGAGGATAAAAATGAAAATGAAGGCTCTGTTGGTGGGCTGAGGAGGAGGAGAAGCAACAAAAGTAAAAGGGATGAAGGGAGTAGCCGAAATAGAAATTCGGAGCTAAATGACAATTTGGCCCATGCATCGCCGCAAGTGCCGCCGCCGCCACCATCTGAGAACCGGCACATTCCACCGCCCCCACAACAAAATTCAACTTACGATTATTTCTTCTCTATGGATTTACCCGTCTCGACTTTCTCTGAAGTTGATGAGGTATACATTAACAGAGAGGAGATTGATATAAAGCCCAAGGTAGTGGACAGTGACGATATTGATGAGCAGAGAAGAAGTGTAAAGGCTGAGACAGTGGAGCCATTGCTCGAGGAGCCGGTGGAGCCGCCTCCCTGCCTGCCTGCAGAACCTGCGACTTCCGTGTCGAAGAGCTCGAAGAAGACGAACCAAGCAGGATCTATGGGGTCCACAGAGGGAAAGAGGATGGTTAAGCCAAATTTGAATTTGTTGCAGATATTTATAGATATTGATGATCATTTTCTCAAGTCTTGTGAGAGTGCCCGTGAAGTGTCCAAGATGCTTGAGGCAACACGATTACACTATCATTCAAACTTTGCTGATAATCGAGGTATCATTCAAGCTTGTTCTTATATCTTTTCTTCAAAAGAATATGATGTACTTTTCTTTAGGTCACATTGACCACTCTGCCAGGGTGATGCGTGTTATTGCATGGAATCGATCATTTAGGGGACTGCCTAATATGGATGATGGAAAAGATGATTTCTATCCAGAAGAACAAGAAACTCATGCAACCGTGTTAGATAAACTACTGGCATGGGAAAAGAAGCTGTCCGATGAAGTGAAGGTATATGGAATACTCTTCTTCCTATCCGAATATTTAAGCATCTCTTTTGGCTCCTCATTTTCACAGACAATCTTCTAACATGATATAATTTCATCATTGGATTGCCAACTCCTTTTAACTCATGAAAAACAATGGAAGTTCATACCAATAGCAAAAATGTGATGTCTTTTTACCATTTTTAGTTATATTCTGTTCTAAAAAATCTGCTCTGTGATTCATGAGCGCTCTTGACACCATCCTTTTGATCCTTGGAGCCTTCAGGTCACCATTTATGCATGTACCTATTTGCAATCTTGTTCTCTTTGAAGTAATAGGCTAGGCAGGCACTATATGGGTTGAGGCTTTTGATGTTCCTTGATCTTATTGAAATAAATTTGTGTTTCTACGATGGAGCAGAGTGGCAACATGATGCGACTAAAAAACTTTCTTTTAACTGAATTTTATTTGTGATATTACGATTGACAGGCCGGTGAACTTATGAAATTTGAATACCAAAAGAAGGTTGCTACATTGAATAGACTGAAGAAACGAGATTCTAATGTAGAAGCATTGGAGAAAGCAAAAGCAGCAGTAAGTCATCTGCACACTAGATATATTGTTGACATGCAATCCTTGGATTCAACTGTCTCAGAGATTAGTCGTCTACGAGACGAACAGTTATACCCAAAACTTGTTCAGCTTGTTAATGGGTGAGTTGCGTGAGAATGCACATGCATATAGTTGTTCATTGTACTTTATTGTTGCTTATTGTCCCTCATTAATATAAGTAAAATGATTCTACATGATTTTGTTGGTTTACATATTTCTACTTTAGATGCACGGTTTCGTGGTATGGTAGCGCATTGCTGACATTCGTATCAAAACCATTTTTATGATTCTAATGTTGTTGTTCCACAGGATGGCGACGATGTGGGATACGATGCGAGCTCATCATGAAATGCAATTGAAGATCGTAAGTGCATTGAGATCGATGGATCTTTCTCAATCCCCAAAAGAAACTAGTACCCATCATTACGAGCGCACGGTTCAGCTGTGTGGTGTTGTGAGAGATTGGCATTCACAGTTTGAGAAGCTTGTGCGGTGTCAAAAAGATTACATTAAAGCCTTAAACAGTTGGTTGAAACAAAATCTAGTTCCTATAGAGAGTAGTTTGAAAGAGAAGGTTTCTTCTCCACCCAGAGCTCAAAGTCCTCCAATTCATAAACTCCTCCTCGCTTGGGATGATCAACTCGATAGACTCCCAGATGAACATCTCAAAACTGCCATATTCACCTTTGGGGGTGTGATTAATACCATTATGCTGCAGCAGGATGACGAGAGGAAATTGATGTTAAAGTGGGAGGAGACCAAGAAAGAGCTCGAGCGCAAGGAGCGACATTTTAACGACTGGCATTACAAATACCAGCAACGAAGAACGCCTGATGAGTTGGACCCTGAAAAGTCTGAAGATAACAGTGAAGATTCCGTAGTTACGGAGAAGTTAATCGTGGTAGAGTCCTTGAAAAGGAGATTAGAAGAGGAAAAAGAAACTCATGCGAAGCAATGCCTTCATGTGAGGGAGAAATCATTGTTAAACCTTAAGAATCAGCTGCCAGAACTCTTCAGGGCTTTGTCAGAATTCTCTTTTTCCAGTTCGGAGATGTACAAGAACTTGAGATCGGTTTGTCAAGTCTAGATTGAAGCAACAAACTTTAAACCAAATCCTA

mRNA sequence

ATCCCAGCTTTTTTTTTTTTTTTTTTCTTTTTTTCTGTTTGGGTTCTCAAATCCTTCATGCTCAAGTCTTGATTCCGCCATCGCCGCCGATTAGCTGAGCTTAATGGGTTGTTCTCAGTCGAAGATCGATAATGAAGAAGCGATAGCCCGTTGTAAAGAACGGAAGATTCATATGAAGGAAGCGGCGACGGCTCGAAACGCTTTCGCCGCTGCGCACTCCGCCTATTCTATGTCGCTGAAAAATATTGGAGCTGCTTTAAGTGATTATGCCCATGGTGAGGTTGAAAATTCCCAATTTGTTGATGTATCTACTCAACCTAATTCTGCCATTACTTCCGCTCCCGCCCCTATTGAACCTTTTCCGCCGCCGCCGCCTTTGCCTCCTTCTAATTTTCCCAATTCTCTTGAAAGGGCGGCCACCATGCCTGAGATGAATGTGCACAAATCCGATCTCAAGCCAGGATCGCCGATTATTGAGGAAGAGGATAAAAATGAAAATGAAGGCTCTGTTGGTGGGCTGAGGAGGAGGAGAAGCAACAAAAGTAAAAGGGATGAAGGGAGTAGCCGAAATAGAAATTCGGAGCTAAATGACAATTTGGCCCATGCATCGCCGCAAGTGCCGCCGCCGCCACCATCTGAGAACCGGCACATTCCACCGCCCCCACAACAAAATTCAACTTACGATTATTTCTTCTCTATGGATTTACCCGTCTCGACTTTCTCTGAAGTTGATGAGGTATACATTAACAGAGAGGAGATTGATATAAAGCCCAAGGTAGTGGACAGTGACGATATTGATGAGCAGAGAAGAAGTGTAAAGGCTGAGACAGTGGAGCCATTGCTCGAGGAGCCGGTGGAGCCGCCTCCCTGCCTGCCTGCAGAACCTGCGACTTCCGTGTCGAAGAGCTCGAAGAAGACGAACCAAGCAGGATCTATGGGGTCCACAGAGGGAAAGAGGATGGTTAAGCCAAATTTGAATTTGTTGCAGATATTTATAGATATTGATGATCATTTTCTCAAGTCTTGTGAGAGTGCCCGTGAAGTGTCCAAGATGCTTGAGGCAACACGATTACACTATCATTCAAACTTTGCTGATAATCGAGGTCACATTGACCACTCTGCCAGGGTGATGCGTGTTATTGCATGGAATCGATCATTTAGGGGACTGCCTAATATGGATGATGGAAAAGATGATTTCTATCCAGAAGAACAAGAAACTCATGCAACCGTGTTAGATAAACTACTGGCATGGGAAAAGAAGCTGTCCGATGAAGTGAAGGCCGGTGAACTTATGAAATTTGAATACCAAAAGAAGGTTGCTACATTGAATAGACTGAAGAAACGAGATTCTAATGTAGAAGCATTGGAGAAAGCAAAAGCAGCAGTAAGTCATCTGCACACTAGATATATTGTTGACATGCAATCCTTGGATTCAACTGTCTCAGAGATTAGTCGTCTACGAGACGAACAGTTATACCCAAAACTTGTTCAGCTTGTTAATGGGATGGCGACGATGTGGGATACGATGCGAGCTCATCATGAAATGCAATTGAAGATCGTAAGTGCATTGAGATCGATGGATCTTTCTCAATCCCCAAAAGAAACTAGTACCCATCATTACGAGCGCACGGTTCAGCTGTGTGGTGTTGTGAGAGATTGGCATTCACAGTTTGAGAAGCTTGTGCGGTGTCAAAAAGATTACATTAAAGCCTTAAACAGTTGGTTGAAACAAAATCTAGTTCCTATAGAGAGTAGTTTGAAAGAGAAGGTTTCTTCTCCACCCAGAGCTCAAAGTCCTCCAATTCATAAACTCCTCCTCGCTTGGGATGATCAACTCGATAGACTCCCAGATGAACATCTCAAAACTGCCATATTCACCTTTGGGGGTGTGATTAATACCATTATGCTGCAGCAGGATGACGAGAGGAAATTGATGTTAAAGTGGGAGGAGACCAAGAAAGAGCTCGAGCGCAAGGAGCGACATTTTAACGACTGGCATTACAAATACCAGCAACGAAGAACGCCTGATGAGTTGGACCCTGAAAAGTCTGAAGATAACAGTGAAGATTCCGTAGTTACGGAGAAGTTAATCGTGGTAGAGTCCTTGAAAAGGAGATTAGAAGAGGAAAAAGAAACTCATGCGAAGCAATGCCTTCATGTGAGGGAGAAATCATTGTTAAACCTTAAGAATCAGCTGCCAGAACTCTTCAGGGCTTTGTCAGAATTCTCTTTTTCCAGTTCGGAGATGTACAAGAACTTGAGATCGGTTTGTCAAGTCTAGATTGAAGCAACAAACTTTAAACCAAATCCTA

Coding sequence (CDS)

ATGGGTTGTTCTCAGTCGAAGATCGATAATGAAGAAGCGATAGCCCGTTGTAAAGAACGGAAGATTCATATGAAGGAAGCGGCGACGGCTCGAAACGCTTTCGCCGCTGCGCACTCCGCCTATTCTATGTCGCTGAAAAATATTGGAGCTGCTTTAAGTGATTATGCCCATGGTGAGGTTGAAAATTCCCAATTTGTTGATGTATCTACTCAACCTAATTCTGCCATTACTTCCGCTCCCGCCCCTATTGAACCTTTTCCGCCGCCGCCGCCTTTGCCTCCTTCTAATTTTCCCAATTCTCTTGAAAGGGCGGCCACCATGCCTGAGATGAATGTGCACAAATCCGATCTCAAGCCAGGATCGCCGATTATTGAGGAAGAGGATAAAAATGAAAATGAAGGCTCTGTTGGTGGGCTGAGGAGGAGGAGAAGCAACAAAAGTAAAAGGGATGAAGGGAGTAGCCGAAATAGAAATTCGGAGCTAAATGACAATTTGGCCCATGCATCGCCGCAAGTGCCGCCGCCGCCACCATCTGAGAACCGGCACATTCCACCGCCCCCACAACAAAATTCAACTTACGATTATTTCTTCTCTATGGATTTACCCGTCTCGACTTTCTCTGAAGTTGATGAGGTATACATTAACAGAGAGGAGATTGATATAAAGCCCAAGGTAGTGGACAGTGACGATATTGATGAGCAGAGAAGAAGTGTAAAGGCTGAGACAGTGGAGCCATTGCTCGAGGAGCCGGTGGAGCCGCCTCCCTGCCTGCCTGCAGAACCTGCGACTTCCGTGTCGAAGAGCTCGAAGAAGACGAACCAAGCAGGATCTATGGGGTCCACAGAGGGAAAGAGGATGGTTAAGCCAAATTTGAATTTGTTGCAGATATTTATAGATATTGATGATCATTTTCTCAAGTCTTGTGAGAGTGCCCGTGAAGTGTCCAAGATGCTTGAGGCAACACGATTACACTATCATTCAAACTTTGCTGATAATCGAGGTCACATTGACCACTCTGCCAGGGTGATGCGTGTTATTGCATGGAATCGATCATTTAGGGGACTGCCTAATATGGATGATGGAAAAGATGATTTCTATCCAGAAGAACAAGAAACTCATGCAACCGTGTTAGATAAACTACTGGCATGGGAAAAGAAGCTGTCCGATGAAGTGAAGGCCGGTGAACTTATGAAATTTGAATACCAAAAGAAGGTTGCTACATTGAATAGACTGAAGAAACGAGATTCTAATGTAGAAGCATTGGAGAAAGCAAAAGCAGCAGTAAGTCATCTGCACACTAGATATATTGTTGACATGCAATCCTTGGATTCAACTGTCTCAGAGATTAGTCGTCTACGAGACGAACAGTTATACCCAAAACTTGTTCAGCTTGTTAATGGGATGGCGACGATGTGGGATACGATGCGAGCTCATCATGAAATGCAATTGAAGATCGTAAGTGCATTGAGATCGATGGATCTTTCTCAATCCCCAAAAGAAACTAGTACCCATCATTACGAGCGCACGGTTCAGCTGTGTGGTGTTGTGAGAGATTGGCATTCACAGTTTGAGAAGCTTGTGCGGTGTCAAAAAGATTACATTAAAGCCTTAAACAGTTGGTTGAAACAAAATCTAGTTCCTATAGAGAGTAGTTTGAAAGAGAAGGTTTCTTCTCCACCCAGAGCTCAAAGTCCTCCAATTCATAAACTCCTCCTCGCTTGGGATGATCAACTCGATAGACTCCCAGATGAACATCTCAAAACTGCCATATTCACCTTTGGGGGTGTGATTAATACCATTATGCTGCAGCAGGATGACGAGAGGAAATTGATGTTAAAGTGGGAGGAGACCAAGAAAGAGCTCGAGCGCAAGGAGCGACATTTTAACGACTGGCATTACAAATACCAGCAACGAAGAACGCCTGATGAGTTGGACCCTGAAAAGTCTGAAGATAACAGTGAAGATTCCGTAGTTACGGAGAAGTTAATCGTGGTAGAGTCCTTGAAAAGGAGATTAGAAGAGGAAAAAGAAACTCATGCGAAGCAATGCCTTCATGTGAGGGAGAAATCATTGTTAAACCTTAAGAATCAGCTGCCAGAACTCTTCAGGGCTTTGTCAGAATTCTCTTTTTCCAGTTCGGAGATGTACAAGAACTTGAGATCGGTTTGTCAAGTCTAG

Protein sequence

MGCSQSKIDNEEAIARCKERKIHMKEAATARNAFAAAHSAYSMSLKNIGAALSDYAHGEVENSQFVDVSTQPNSAITSAPAPIEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPGSPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLAHASPQVPPPPPSENRHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINREEIDIKPKVVDSDDIDEQRRSVKAETVEPLLEEPVEPPPCLPAEPATSVSKSSKKTNQAGSMGSTEGKRMVKPNLNLLQIFIDIDDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVIAWNRSFRGLPNMDDGKDDFYPEEQETHATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVATLNRLKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHEMQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSWLKQNLVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLDRLPDEHLKTAIFTFGGVINTIMLQQDDERKLMLKWEETKKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNSEDSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLRSVCQV
Homology
BLAST of CmaCh01G001140 vs. ExPASy Swiss-Prot
Match: Q9AQW1 (Protein ROLLING AND ERECT LEAF 2 OS=Oryza sativa subsp. japonica OX=39947 GN=REL2 PE=2 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 3.7e-57
Identity = 227/767 (29.60%), Postives = 346/767 (45.11%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAIARCKERKIHMKEAATARNAFAAAHSAYSMSLKNIGAALSDYAHGEV 60
           MGC+ SK++ E+ + RCKER+ HMKEA  +R   A+AH+ Y  SL+   AALS +A G  
Sbjct: 1   MGCTASKVEQEDTVRRCKERRRHMKEAVASRQQLASAHADYLRSLRLTAAALSRFAQG-- 60

Query: 61  ENSQFVDVSTQPNSAITSAPAPIEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPG 120
             S  V   T P    T+APA + P P PPP P S   +SL      P +  H+    P 
Sbjct: 61  HPSLAVSHHTAPVLLTTAAPA-LAPTPTPPP-PSSTASSSL--PPPTPLLPKHQQAPPPP 120

Query: 121 SPIIEEEDKN--ENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLAHASPQVPPPPPS 180
            P    +           GG RR +      D   +    S          P V  P  S
Sbjct: 121 PPTQSHQPPPPVAVRAPRGGPRRLKVPHILSDSSVASPARSSFR------KPVVGTPSSS 180

Query: 181 E----NRHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEV-----YINREEIDIKPKVVDSD 240
                    PP P  +  +D   +     +   E++E      Y++   +  + +V D D
Sbjct: 181 SAWDWENFYPPSPPDSEFFDRRKADLEEANRLRELEEEEKARGYLHPHHLKEEDEVDDDD 240

Query: 241 D-----------IDEQRRSVKAETVEPLLEEPV-----------------EPPPCLPAEP 300
           D            D+        T E   EE                     P    A P
Sbjct: 241 DEREEEMHCGGWEDDDDHYASTTTSETRSEEGEMGNRSECGFAARSEYGGTAPSEYAAAP 300

Query: 301 ATSVSKSSKKTNQAGSMGST----EGKRMVKPNLNLLQIFIDIDDHFLKSCESAREVSKM 360
                +   + ++AG   ST       RMV  +  L +I   I+++F+K+ E+   VS++
Sbjct: 301 LPLPLRRRDERSEAGDSSSTVTAAAEMRMVIRHRTLAEIVAAIEEYFVKAAEAGNGVSEL 360

Query: 361 LEATRLHYHSNFADNRGHIDHSARVMRVIA--WNRS--FRGLPNMDDGKDDFYPEEQETH 420
           LEA+R     NF   +  + HS  ++  ++  W           +D    +    E ++H
Sbjct: 361 LEASRAQLDRNFRQLKKTVYHSNSLLSSLSSTWTSKPPLAVRYKLDTNALEMESMEGKSH 420

Query: 421 ATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVATLNRLKKRDSNVEALEKAKAAVSHLHT 480
            + L++LLAWEKKL  EVKA E +K E++KK++TL  L+ R  +   L+K KA+++ L +
Sbjct: 421 GSTLERLLAWEKKLYQEVKARESVKIEHEKKLSTLQSLEYRGRDSTKLDKTKASINKLQS 480

Query: 481 RYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHEMQLKIVSALRSMD 540
             IV  Q+  +T S I R+RD +L P+LV+L   + +MW +M   HE+Q +IV  +R + 
Sbjct: 481 LIIVTSQAATTTSSAIVRVRDNELAPQLVELCFALLSMWRSMNHFHEIQNEIVQQVRGLV 540

Query: 541 LSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSWLKQNLVPIESSLK 600
            +   + TS  H   T  L   V  WHS F +L++ Q+DYI+AL  WLK  L  ++S++ 
Sbjct: 541 DNSMAESTSDLHRLATRDLEAAVSAWHSNFNRLIKYQRDYIRALYGWLKLTLFQVDSNI- 600

Query: 601 EKVSSPPRAQSPPIHKLLLA----WDDQLDRLPDEHLKTAIFTFGGVINTIMLQQDDERK 660
                P  A +  I + L      W   LDRLPD     AI +F  V++ I  +Q +E K
Sbjct: 601 -----PQEAYTSLISRELTTFCDEWKQALDRLPDASASEAIKSFVNVVHVIYTKQAEEMK 660

Query: 661 LMLKWEETKKELERKERHFNDWHYKYQQRRTPDELD-PEKSEDNSED------SVVTEKL 710
           +  + E   KELE+K         KY Q  +   L  P    D  E         + EK 
Sbjct: 661 IKKRTETYSKELEKKTNSLRAIEKKYYQSYSMVGLGLPGSGRDGIESHSFDARDPLAEKK 720

BLAST of CmaCh01G001140 vs. ExPASy Swiss-Prot
Match: A0A178VBJ0 (Protein ALTERED PHOSPHATE STARVATION RESPONSE 1 OS=Arabidopsis thaliana OX=3702 GN=APSR1 PE=2 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 2.0e-55
Identity = 197/731 (26.95%), Postives = 316/731 (43.23%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAIARCKERKIHMKEAATARNAFAAAHSAYSMSLKNIGAALSDYAHGEV 60
           MGC QS+ID++E ++RCK RK ++K    AR   + +H+ Y  SL+ +G++L  +     
Sbjct: 1   MGCCQSRIDSKEIVSRCKARKRYLKHLVKARQTLSVSHALYLRSLRAVGSSLVHF----- 60

Query: 61  ENSQFVDVSTQPNSAITSAPAPIEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPG 120
            +S+   +    N    S P P  P PPPPPL P +   +     T              
Sbjct: 61  -SSKETPLHLHHNPPSPSPPPPPPPRPPPPPLSPGSETTTWTTTTT-------------- 120

Query: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLAHASPQVPPPPPSEN 180
                                                           S  +PPPPP   
Sbjct: 121 ------------------------------------------------SSVLPPPPPPP- 180

Query: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINREEIDIKPKVVDSDDIDEQRRSVKA 240
              PPPP  +ST+D++     P  + SE +      EE     +       D       A
Sbjct: 181 ---PPPPPPSSTWDFWDPFIPPPPSSSEEEW----EEETTTATRTATGTGSD------AA 240

Query: 241 ETVEPLLEEPVEPPPCLPAEPATSVSKSSKKTNQAGSMGSTEGKRMVKPNLNLLQIFIDI 300
            T  P    P         + ++ VS  SK T    + GS     + +   +L++I  ++
Sbjct: 241 VTTAPTTATP---------QASSVVSGFSKDTMTTTTTGSELAVVVSRNGKDLMEIIKEV 300

Query: 301 DDHFLKSCESAREVSKMLE----ATRLHYHSNFADNRGHIDHSARVMRVIAWNRSF---- 360
           D++FLK+ +S   +S +LE     T    HS         ++   +     W R F    
Sbjct: 301 DEYFLKAADSGAPLSSLLEISTSITDFSGHSKSGKMYSSSNYECNLNPTSFWTRGFAPSK 360

Query: 361 ----RGLPNMDDGKDDFYPEEQETHATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVATL 420
               R    +  G          +H++ +D+L AWEKKL  EVK  E +K +++KKV  +
Sbjct: 361 LSEYRNAGGVIGGNCIV-----GSHSSTVDRLYAWEKKLYQEVKYAESIKMDHEKKVEQV 420

Query: 421 NRLKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGM 480
            RL+ + +     EKAK  V  L ++  V  Q++ S  +EI +LR+ +LYP+LV+LV G+
Sbjct: 421 RRLEMKRAEYVKTEKAKKDVEKLESQLSVSSQAIQSASNEIIKLRETELYPQLVELVKGL 480

Query: 481 ATMWDTMRAHHEMQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVR 540
             MW +M   H++Q  IV  L+ ++   S + TS  H + T+QL   V+ WH  F  LV+
Sbjct: 481 MCMWRSMYESHQVQTHIVQQLKYLNTIPSTEPTSELHRQSTLQLELEVQQWHHSFCNLVK 540

Query: 541 CQKDYIKALNSWLKQNLVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLDRLPDEHLKT 600
            Q+DYI++L  WL+ +L     +   + S   +     I+     W   +DR+PD+    
Sbjct: 541 AQRDYIQSLTGWLRLSLFQFSKNPLVRSSYESK-----IYSFCEEWHLAIDRIPDKVASE 600

Query: 601 AIFTFGGVINTIMLQQDDERKLMLKWEETKKELERKERHFNDWHYKYQQRRTPDELDPEK 660
            I +F   ++ I+ QQ DE K   + E   K+ E+K         KY     P       
Sbjct: 601 GIKSFLTAVHGIVAQQADEHKQKKRTESMLKDFEKKSASLRALESKYSPYSVP------- 621

Query: 661 SEDNSEDSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEF 720
             ++ + + V EK + VE LK + EEEK  H K     R  +L NL+   P +F+A+  F
Sbjct: 661 --ESRKKNPVIEKRVKVEMLKGKAEEEKSKHEKSVSVTRAMTLNNLQMGFPHVFQAMVGF 621

BLAST of CmaCh01G001140 vs. ExPASy Swiss-Prot
Match: Q93YU8 (Nitrate regulatory gene2 protein OS=Arabidopsis thaliana OX=3702 GN=NRG2 PE=1 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 8.6e-54
Identity = 222/801 (27.72%), Postives = 366/801 (45.69%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAIARCKERKIHMKEAATARNAFAAAHSAYSMSLKNIGAALSDYAHGEV 60
           MGC+ SK+DNE+A+ RCK+R+  MKEA  AR+  AAAH+ Y  SL+  G+ALS +A GE 
Sbjct: 1   MGCAASKLDNEDAVRRCKDRRRLMKEAVYARHHLAAAHADYCRSLRITGSALSSFASGEP 60

Query: 61  ENSQFVDVSTQ-PNSAITSAPAPIEPFPP----PPPLPPSNFPNSLERAATMPEMNVHKS 120
                + VS Q P   + + P P+    P    PP   PS  P+S+   +T P +   K 
Sbjct: 61  -----LSVSDQTPAVFLHTPPPPLSEQSPAKFVPPRFSPSPAPSSVYPPSTSPSVASSKQ 120

Query: 121 DLKPGSPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGS--------SRNRNSELNDNLAH 180
                +       +         L     + S R E S        S  +NS  +   +H
Sbjct: 121 PSVMSTSSNRRRKQQPKPRLPHILSESSPSSSPRSERSNFMPNLYPSAYQNSTYSATPSH 180

Query: 181 ASPQ------VPPPPP---------------SENRHIPPPPQQ-NSTYDYFFSMDLPVST 240
           AS         PP PP               S+NR      +   S YD+F +       
Sbjct: 181 ASSVWNWENFYPPSPPDSEFFNRKAQEKKHNSDNRFNDEDTETVRSEYDFFDTRKQKQKQ 240

Query: 241 FSEV-----DEVYINREEIDI-------------KPKVVDSDDIDEQRRS---------- 300
           F  +     +E    REE+                    + ++ D+ R S          
Sbjct: 241 FESMRNQVEEETETEREEVQCSEWEDHDHYSTTSSSDAAEEEEEDDDRESISEVGTRSEF 300

Query: 301 ---VKAETVEPLLEEPVEPPPCLPAEPATSVSKSSKKTNQAGSM---GSTEGKRMVKPNL 360
              V++ ++    ++P   P        +   K+   T  +GS    G     +MV  + 
Sbjct: 301 GSTVRSNSMRRHHQQPSPMPQVYGGAEQSKYDKADDATISSGSYRGGGDIADMKMVVRHR 360

Query: 361 NLLQIFIDIDDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVIA--W- 420
           +L +I   I ++F K+  S  +VS+MLE  R     +F+  +  + HS+ ++  ++  W 
Sbjct: 361 DLKEIIDAIKENFDKAAASGEQVSQMLELGRAELDRSFSQLKKTVIHSSSLLSNLSSTWT 420

Query: 421 NRSFRGLPNMDDGKDDFYPEEQETHATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVATL 480
           ++    +    D      P   ++  + LD+LLAWEKKL +E+KA E  K E++KK++ L
Sbjct: 421 SKPPLAVKYRIDTTALDQPNSSKSLCSTLDRLLAWEKKLYEEIKAREGFKIEHEKKLSQL 480

Query: 481 NRLKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGM 540
              + +  +   L+K KA+++ L +  IV  Q++ +T + I RLRD  L P+LV+L +G 
Sbjct: 481 QSQEYKGEDEAKLDKTKASITRLQSLIIVTSQAVTTTSTAIIRLRDTDLVPQLVELCHGF 540

Query: 541 ATMWDTMRAHHEMQLKIVSALRSM-DLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLV 600
             MW +M  +HE Q  IV  +R + + S   + TS  H + T  L   V  WHS F  L+
Sbjct: 541 MYMWKSMHQYHETQNSIVEQVRGLINRSGKGESTSELHRQATRDLESAVSSWHSSFSSLI 600

Query: 601 RCQKDYIKALNSWLKQNLVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLDRLPDEHLK 660
           + Q+D+I ++++W K  L+P+     ++ ++    +    +     W   LDR+PD    
Sbjct: 601 KFQRDFIHSVHAWFKLTLLPV----CQEDAANHHKEPLDAYAFCDEWKLALDRIPDTVAS 660

Query: 661 TAIFTFGGVINTIMLQQDDERKLMLKWEETKKELERKERHFNDWHYKYQQRRTPDELD-P 720
            AI +F  V++ I  +Q DE K+  + E   KELE+K     +   KY Q  +   +  P
Sbjct: 661 EAIKSFINVVHVISAKQADEHKIKKRTESASKELEKKASSVRNLERKYYQSYSMVGVGLP 720

Query: 721 EKSEDNSE----DSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELF 724
           E   DN         +++K   +   +RR+EEE   ++K     R  +L NL+  LP +F
Sbjct: 721 ESGPDNQHMLDARDPLSDKKSELAVCQRRVEEEMVKYSKAIEVTRAMTLNNLQTGLPGVF 780

BLAST of CmaCh01G001140 vs. ExPASy TrEMBL
Match: A0A6J1K8S0 (nitrate regulatory gene2 protein-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493306 PE=4 SV=1)

HSP 1 Score: 1406.3 bits (3639), Expect = 0.0e+00
Identity = 725/725 (100.00%), Postives = 725/725 (100.00%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAIARCKERKIHMKEAATARNAFAAAHSAYSMSLKNIGAALSDYAHGEV 60
           MGCSQSKIDNEEAIARCKERKIHMKEAATARNAFAAAHSAYSMSLKNIGAALSDYAHGEV
Sbjct: 1   MGCSQSKIDNEEAIARCKERKIHMKEAATARNAFAAAHSAYSMSLKNIGAALSDYAHGEV 60

Query: 61  ENSQFVDVSTQPNSAITSAPAPIEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPG 120
           ENSQFVDVSTQPNSAITSAPAPIEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPG
Sbjct: 61  ENSQFVDVSTQPNSAITSAPAPIEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPG 120

Query: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLAHASPQVPPPPPSEN 180
           SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLAHASPQVPPPPPSEN
Sbjct: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLAHASPQVPPPPPSEN 180

Query: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINREEIDIKPKVVDSDDIDEQRRSVKA 240
           RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINREEIDIKPKVVDSDDIDEQRRSVKA
Sbjct: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINREEIDIKPKVVDSDDIDEQRRSVKA 240

Query: 241 ETVEPLLEEPVEPPPCLPAEPATSVSKSSKKTNQAGSMGSTEGKRMVKPNLNLLQIFIDI 300
           ETVEPLLEEPVEPPPCLPAEPATSVSKSSKKTNQAGSMGSTEGKRMVKPNLNLLQIFIDI
Sbjct: 241 ETVEPLLEEPVEPPPCLPAEPATSVSKSSKKTNQAGSMGSTEGKRMVKPNLNLLQIFIDI 300

Query: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVIAWNRSFRGLPNMDD 360
           DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVIAWNRSFRGLPNMDD
Sbjct: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVIAWNRSFRGLPNMDD 360

Query: 361 GKDDFYPEEQETHATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVATLNRLKKRDSNVEA 420
           GKDDFYPEEQETHATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVATLNRLKKRDSNVEA
Sbjct: 361 GKDDFYPEEQETHATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVATLNRLKKRDSNVEA 420

Query: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHE 480
           LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHE
Sbjct: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHE 480

Query: 481 MQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540
           MQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW
Sbjct: 481 MQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540

Query: 541 LKQNLVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLDRLPDEHLKTAIFTFGGVINTI 600
           LKQNLVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLDRLPDEHLKTAIFTFGGVINTI
Sbjct: 541 LKQNLVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLDRLPDEHLKTAIFTFGGVINTI 600

Query: 601 MLQQDDERKLMLKWEETKKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNSEDSVVTE 660
           MLQQDDERKLMLKWEETKKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNSEDSVVTE
Sbjct: 601 MLQQDDERKLMLKWEETKKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNSEDSVVTE 660

Query: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR 720
           KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR
Sbjct: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR 720

Query: 721 SVCQV 726
           SVCQV
Sbjct: 721 SVCQV 725

BLAST of CmaCh01G001140 vs. ExPASy TrEMBL
Match: A0A6J1G9I1 (nitrate regulatory gene2 protein-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111452183 PE=4 SV=1)

HSP 1 Score: 1347.0 bits (3485), Expect = 0.0e+00
Identity = 694/725 (95.72%), Postives = 709/725 (97.79%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAIARCKERKIHMKEAATARNAFAAAHSAYSMSLKNIGAALSDYAHGEV 60
           MGCSQSKIDNEEA+ARCKERKIHMKEA T RNAFAAAHSAYSMSLKNIGA LSDYAHGEV
Sbjct: 1   MGCSQSKIDNEEAVARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAVLSDYAHGEV 60

Query: 61  ENSQFVDVSTQPNSAITSAPAPIEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPG 120
           EN QFV VSTQPNSAITSAPAP EPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKP 
Sbjct: 61  ENPQFVYVSTQPNSAITSAPAPSEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPE 120

Query: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLAHASPQVPPPPPSEN 180
           SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLA+ASPQVPPPP SEN
Sbjct: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLANASPQVPPPPQSEN 180

Query: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINREEIDIKPKVVDSDDIDEQRRSVKA 240
           RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYIN+EEIDIKPKVVDSDDIDEQR+SVKA
Sbjct: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVVDSDDIDEQRKSVKA 240

Query: 241 ETVEPLLEEPVEPPPCLPAEPATSVSKSSKKTNQAGSMGSTEGKRMVKPNLNLLQIFIDI 300
           ET EPLLEEPVEPPP LPAEPAT+V+KSSKK NQAGSMGSTEGKRMVKP+LNLLQIFIDI
Sbjct: 241 ETAEPLLEEPVEPPPSLPAEPATAVAKSSKKANQAGSMGSTEGKRMVKPDLNLLQIFIDI 300

Query: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVIAWNRSFRGLPNMDD 360
           DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVI WNRSFRGLPNMDD
Sbjct: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMDD 360

Query: 361 GKDDFYPEEQETHATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVATLNRLKKRDSNVEA 420
           GKDD Y EEQETHATVLDKLLAWEKKL DEVKAGE+MKFEYQKKVATLNRLKKRDSNVEA
Sbjct: 361 GKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEA 420

Query: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHE 480
           LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMAT+WDTMRAHHE
Sbjct: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATVWDTMRAHHE 480

Query: 481 MQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540
           MQLKIVSALR MDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW
Sbjct: 481 MQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540

Query: 541 LKQNLVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLDRLPDEHLKTAIFTFGGVINTI 600
           LKQN+VPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQL+RLPDEHLKTAIFTFGGVI+TI
Sbjct: 541 LKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVISTI 600

Query: 601 MLQQDDERKLMLKWEETKKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNSEDSVVTE 660
           MLQQDDERKLMLKWEET+KELERKERHFNDWHYKYQQRRTPDELDPEKSEDN++DSVVTE
Sbjct: 601 MLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDSVVTE 660

Query: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR 720
           KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR
Sbjct: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR 720

Query: 721 SVCQV 726
           SVCQV
Sbjct: 721 SVCQV 725

BLAST of CmaCh01G001140 vs. ExPASy TrEMBL
Match: A0A6J1KHK4 (nitrate regulatory gene2 protein-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493306 PE=4 SV=1)

HSP 1 Score: 1207.2 bits (3122), Expect = 0.0e+00
Identity = 619/619 (100.00%), Postives = 619/619 (100.00%), Query Frame = 0

Query: 107 MPEMNVHKSDLKPGSPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLA 166
           MPEMNVHKSDLKPGSPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLA
Sbjct: 1   MPEMNVHKSDLKPGSPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLA 60

Query: 167 HASPQVPPPPPSENRHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINREEIDIKPKVV 226
           HASPQVPPPPPSENRHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINREEIDIKPKVV
Sbjct: 61  HASPQVPPPPPSENRHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINREEIDIKPKVV 120

Query: 227 DSDDIDEQRRSVKAETVEPLLEEPVEPPPCLPAEPATSVSKSSKKTNQAGSMGSTEGKRM 286
           DSDDIDEQRRSVKAETVEPLLEEPVEPPPCLPAEPATSVSKSSKKTNQAGSMGSTEGKRM
Sbjct: 121 DSDDIDEQRRSVKAETVEPLLEEPVEPPPCLPAEPATSVSKSSKKTNQAGSMGSTEGKRM 180

Query: 287 VKPNLNLLQIFIDIDDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVI 346
           VKPNLNLLQIFIDIDDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVI
Sbjct: 181 VKPNLNLLQIFIDIDDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVI 240

Query: 347 AWNRSFRGLPNMDDGKDDFYPEEQETHATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVA 406
           AWNRSFRGLPNMDDGKDDFYPEEQETHATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVA
Sbjct: 241 AWNRSFRGLPNMDDGKDDFYPEEQETHATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVA 300

Query: 407 TLNRLKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVN 466
           TLNRLKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVN
Sbjct: 301 TLNRLKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVN 360

Query: 467 GMATMWDTMRAHHEMQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKL 526
           GMATMWDTMRAHHEMQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKL
Sbjct: 361 GMATMWDTMRAHHEMQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKL 420

Query: 527 VRCQKDYIKALNSWLKQNLVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLDRLPDEHL 586
           VRCQKDYIKALNSWLKQNLVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLDRLPDEHL
Sbjct: 421 VRCQKDYIKALNSWLKQNLVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLDRLPDEHL 480

Query: 587 KTAIFTFGGVINTIMLQQDDERKLMLKWEETKKELERKERHFNDWHYKYQQRRTPDELDP 646
           KTAIFTFGGVINTIMLQQDDERKLMLKWEETKKELERKERHFNDWHYKYQQRRTPDELDP
Sbjct: 481 KTAIFTFGGVINTIMLQQDDERKLMLKWEETKKELERKERHFNDWHYKYQQRRTPDELDP 540

Query: 647 EKSEDNSEDSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALS 706
           EKSEDNSEDSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALS
Sbjct: 541 EKSEDNSEDSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALS 600

Query: 707 EFSFSSSEMYKNLRSVCQV 726
           EFSFSSSEMYKNLRSVCQV
Sbjct: 601 EFSFSSSEMYKNLRSVCQV 619

BLAST of CmaCh01G001140 vs. ExPASy TrEMBL
Match: A0A6J1G9J8 (nitrate regulatory gene2 protein-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452183 PE=4 SV=1)

HSP 1 Score: 1160.6 bits (3001), Expect = 0.0e+00
Identity = 595/619 (96.12%), Postives = 609/619 (98.38%), Query Frame = 0

Query: 107 MPEMNVHKSDLKPGSPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLA 166
           MPEMNVHKSDLKP SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLA
Sbjct: 1   MPEMNVHKSDLKPESPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLA 60

Query: 167 HASPQVPPPPPSENRHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINREEIDIKPKVV 226
           +ASPQVPPPP SENRHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYIN+EEIDIKPKVV
Sbjct: 61  NASPQVPPPPQSENRHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVV 120

Query: 227 DSDDIDEQRRSVKAETVEPLLEEPVEPPPCLPAEPATSVSKSSKKTNQAGSMGSTEGKRM 286
           DSDDIDEQR+SVKAET EPLLEEPVEPPP LPAEPAT+V+KSSKK NQAGSMGSTEGKRM
Sbjct: 121 DSDDIDEQRKSVKAETAEPLLEEPVEPPPSLPAEPATAVAKSSKKANQAGSMGSTEGKRM 180

Query: 287 VKPNLNLLQIFIDIDDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVI 346
           VKP+LNLLQIFIDIDDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVI
Sbjct: 181 VKPDLNLLQIFIDIDDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVI 240

Query: 347 AWNRSFRGLPNMDDGKDDFYPEEQETHATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVA 406
            WNRSFRGLPNMDDGKDD Y EEQETHATVLDKLLAWEKKL DEVKAGE+MKFEYQKKVA
Sbjct: 241 TWNRSFRGLPNMDDGKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVA 300

Query: 407 TLNRLKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVN 466
           TLNRLKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVN
Sbjct: 301 TLNRLKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVN 360

Query: 467 GMATMWDTMRAHHEMQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKL 526
           GMAT+WDTMRAHHEMQLKIVSALR MDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKL
Sbjct: 361 GMATVWDTMRAHHEMQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKL 420

Query: 527 VRCQKDYIKALNSWLKQNLVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLDRLPDEHL 586
           VRCQKDYIKALNSWLKQN+VPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQL+RLPDEHL
Sbjct: 421 VRCQKDYIKALNSWLKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHL 480

Query: 587 KTAIFTFGGVINTIMLQQDDERKLMLKWEETKKELERKERHFNDWHYKYQQRRTPDELDP 646
           KTAIFTFGGVI+TIMLQQDDERKLMLKWEET+KELERKERHFNDWHYKYQQRRTPDELDP
Sbjct: 481 KTAIFTFGGVISTIMLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDP 540

Query: 647 EKSEDNSEDSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALS 706
           EKSEDN++DSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALS
Sbjct: 541 EKSEDNTQDSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALS 600

Query: 707 EFSFSSSEMYKNLRSVCQV 726
           EFSFSSSEMYKNLRSVCQV
Sbjct: 601 EFSFSSSEMYKNLRSVCQV 619

BLAST of CmaCh01G001140 vs. ExPASy TrEMBL
Match: A0A6J1ISF0 (nitrate regulatory gene2 protein-like OS=Cucurbita maxima OX=3661 GN=LOC111480123 PE=4 SV=1)

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 605/735 (82.31%), Postives = 662/735 (90.07%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAIARCKERKIHMKEAATARNAFAAAHSAYSMSLKNIGAALSDYAHGEV 60
           MGCSQSKI+NEEAIARCKERKIHMKEA T RNAFAAAHSAYSMSLKN GAALSDYAHGEV
Sbjct: 1   MGCSQSKIENEEAIARCKERKIHMKEAVTFRNAFAAAHSAYSMSLKNTGAALSDYAHGEV 60

Query: 61  ENSQFVDVSTQPNSAITSA-PAPIEPF-PPPPPLPPSNFPNSLERAATMPEMNVHKSDLK 120
           +N+QFV VSTQPNSA+TSA  A  EPF PPPPPLPPSNF + L+RAATMPE+N++K DLK
Sbjct: 61  QNTQFVPVSTQPNSALTSATAASFEPFPPPPPPLPPSNFHSPLQRAATMPEINMYKPDLK 120

Query: 121 PGSPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNS-ELNDNLAHASPQVPPPPP 180
           PGSPIIEEE++NENEGSVG LRRRRSNKSK DEGSSRNRNS ELN++LA ASP V PPPP
Sbjct: 121 PGSPIIEEEEENENEGSVGALRRRRSNKSKGDEGSSRNRNSAELNEDLAGASPPV-PPPP 180

Query: 181 SENRHIPPPPQQNSTYDYFFSMD-LPVSTFSEVDEVYINREEIDIK-----PKVVDSDDI 240
           SENRHIPPPPQQ+STYDYFFS+D +PVST SEV+EV IN+ EI+ K      K VD+ DI
Sbjct: 181 SENRHIPPPPQQDSTYDYFFSVDNIPVSTLSEVEEVQINKAEIERKSFDKMSKGVDNHDI 240

Query: 241 DEQRRSVKAETVEPLLEEPVEPPPCLPAEPATSV-SKSSKKTNQAGSMGSTEGKRMVKPN 300
           +E+  S KAETVE +LEEPV PPP  P+   +SV +KS KK  Q GSMG+ +GKRMVKPN
Sbjct: 241 EERGISGKAETVESVLEEPVAPPPAPPSVAESSVAAKSLKKMKQGGSMGAMDGKRMVKPN 300

Query: 301 LNLLQIFIDIDDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVIAWNR 360
           +NLL IF +IDD+FL++ ESA EVSKMLEATRLHYHSNFADNRGHIDHSARVMRVI WNR
Sbjct: 301 VNLLLIFTNIDDNFLQASESAHEVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNR 360

Query: 361 SFRGLPNMDDGKDDFYPEEQETHATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVATLNR 420
           SFRGL NMDDGKDDFY EEQETHATVLDKLLAWEKKL DEVKAGELMKFEYQKKVATLNR
Sbjct: 361 SFRGLANMDDGKDDFYAEEQETHATVLDKLLAWEKKLYDEVKAGELMKFEYQKKVATLNR 420

Query: 421 LKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMAT 480
           LKKRDSN EALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGM+ 
Sbjct: 421 LKKRDSNAEALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMSL 480

Query: 481 MWDTMRAHHEMQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQ 540
           MWDTMRAHHE QLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVR+WHSQFEKLVRCQ
Sbjct: 481 MWDTMRAHHETQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVREWHSQFEKLVRCQ 540

Query: 541 KDYIKALNSWLKQNLVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLDRLPDEHLKTAI 600
           KDYI+ALNSWLK NL+PIESSL+EKVSSPPR QSPPI KLL+AW DQL+RLPDEHL+TAI
Sbjct: 541 KDYIRALNSWLKLNLIPIESSLREKVSSPPRVQSPPIQKLLIAWHDQLERLPDEHLRTAI 600

Query: 601 FTFGGVINTIMLQQDDERKLMLKWEETKKELERKERHFNDWHYKYQQRRTPDELDPEKSE 660
           FTFG VINTIMLQQD+ERKL  KWEET KELERK+RHFN+WH KYQQRR PDELDPE+SE
Sbjct: 601 FTFGAVINTIMLQQDEERKLKTKWEETGKELERKQRHFNEWHNKYQQRRMPDELDPERSE 660

Query: 661 DNSEDSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSF 720
           +N++D+ VT+KL+ VE LK+RLEEE ETHAKQCLHVREKSL++LKNQLP+LFRALSEFS 
Sbjct: 661 ENTQDAAVTDKLVAVELLKKRLEEEIETHAKQCLHVREKSLVSLKNQLPDLFRALSEFSL 720

Query: 721 SSSEMYKNLRSVCQV 726
           +SSEMYKNLRS+CQV
Sbjct: 721 ASSEMYKNLRSICQV 734

BLAST of CmaCh01G001140 vs. NCBI nr
Match: XP_022998727.1 (nitrate regulatory gene2 protein-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1406.3 bits (3639), Expect = 0.0e+00
Identity = 725/725 (100.00%), Postives = 725/725 (100.00%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAIARCKERKIHMKEAATARNAFAAAHSAYSMSLKNIGAALSDYAHGEV 60
           MGCSQSKIDNEEAIARCKERKIHMKEAATARNAFAAAHSAYSMSLKNIGAALSDYAHGEV
Sbjct: 1   MGCSQSKIDNEEAIARCKERKIHMKEAATARNAFAAAHSAYSMSLKNIGAALSDYAHGEV 60

Query: 61  ENSQFVDVSTQPNSAITSAPAPIEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPG 120
           ENSQFVDVSTQPNSAITSAPAPIEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPG
Sbjct: 61  ENSQFVDVSTQPNSAITSAPAPIEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPG 120

Query: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLAHASPQVPPPPPSEN 180
           SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLAHASPQVPPPPPSEN
Sbjct: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLAHASPQVPPPPPSEN 180

Query: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINREEIDIKPKVVDSDDIDEQRRSVKA 240
           RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINREEIDIKPKVVDSDDIDEQRRSVKA
Sbjct: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINREEIDIKPKVVDSDDIDEQRRSVKA 240

Query: 241 ETVEPLLEEPVEPPPCLPAEPATSVSKSSKKTNQAGSMGSTEGKRMVKPNLNLLQIFIDI 300
           ETVEPLLEEPVEPPPCLPAEPATSVSKSSKKTNQAGSMGSTEGKRMVKPNLNLLQIFIDI
Sbjct: 241 ETVEPLLEEPVEPPPCLPAEPATSVSKSSKKTNQAGSMGSTEGKRMVKPNLNLLQIFIDI 300

Query: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVIAWNRSFRGLPNMDD 360
           DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVIAWNRSFRGLPNMDD
Sbjct: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVIAWNRSFRGLPNMDD 360

Query: 361 GKDDFYPEEQETHATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVATLNRLKKRDSNVEA 420
           GKDDFYPEEQETHATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVATLNRLKKRDSNVEA
Sbjct: 361 GKDDFYPEEQETHATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVATLNRLKKRDSNVEA 420

Query: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHE 480
           LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHE
Sbjct: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHE 480

Query: 481 MQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540
           MQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW
Sbjct: 481 MQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540

Query: 541 LKQNLVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLDRLPDEHLKTAIFTFGGVINTI 600
           LKQNLVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLDRLPDEHLKTAIFTFGGVINTI
Sbjct: 541 LKQNLVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLDRLPDEHLKTAIFTFGGVINTI 600

Query: 601 MLQQDDERKLMLKWEETKKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNSEDSVVTE 660
           MLQQDDERKLMLKWEETKKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNSEDSVVTE
Sbjct: 601 MLQQDDERKLMLKWEETKKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNSEDSVVTE 660

Query: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR 720
           KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR
Sbjct: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR 720

Query: 721 SVCQV 726
           SVCQV
Sbjct: 721 SVCQV 725

BLAST of CmaCh01G001140 vs. NCBI nr
Match: XP_023524816.1 (nitrate regulatory gene2 protein-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1355.9 bits (3508), Expect = 0.0e+00
Identity = 699/725 (96.41%), Postives = 711/725 (98.07%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAIARCKERKIHMKEAATARNAFAAAHSAYSMSLKNIGAALSDYAHGEV 60
           MGCSQSKIDNEEAIARCKERKIHMKEA T RNAFAAAHSAYSMSLKNIGAALSDYAHGEV
Sbjct: 1   MGCSQSKIDNEEAIARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAALSDYAHGEV 60

Query: 61  ENSQFVDVSTQPNSAITSAPAPIEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPG 120
           EN QFVDVSTQPNSAITSAPAPIEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPG
Sbjct: 61  ENPQFVDVSTQPNSAITSAPAPIEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPG 120

Query: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLAHASPQVPPPPPSEN 180
           SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELND LA+ASPQVPPPPPSEN
Sbjct: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDILANASPQVPPPPPSEN 180

Query: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINREEIDIKPKVVDSDDIDEQRRSVKA 240
           RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYIN+EEIDIKPKVVD DDIDE+RRSVKA
Sbjct: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVVDIDDIDERRRSVKA 240

Query: 241 ETVEPLLEEPVEPPPCLPAEPATSVSKSSKKTNQAGSMGSTEGKRMVKPNLNLLQIFIDI 300
           ET EPLLEEPVEPPP LPAEPAT+V+KSSKKTNQAGSMGS EGKRMVKPNLNLLQIFIDI
Sbjct: 241 ETAEPLLEEPVEPPPSLPAEPATAVAKSSKKTNQAGSMGSIEGKRMVKPNLNLLQIFIDI 300

Query: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVIAWNRSFRGLPNMDD 360
           DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVI WNRSFRGLPNMDD
Sbjct: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMDD 360

Query: 361 GKDDFYPEEQETHATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVATLNRLKKRDSNVEA 420
           GKDD Y EEQETHATVLDKLLAWEKKL DEVKAGE+MKFEYQKKVATLNRLKKRDSNVEA
Sbjct: 361 GKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEA 420

Query: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHE 480
           LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHE
Sbjct: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHE 480

Query: 481 MQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540
           MQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW
Sbjct: 481 MQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540

Query: 541 LKQNLVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLDRLPDEHLKTAIFTFGGVINTI 600
           LKQN+VPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQL+RLPDEHLKTAIFTFGGVINTI
Sbjct: 541 LKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVINTI 600

Query: 601 MLQQDDERKLMLKWEETKKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNSEDSVVTE 660
           MLQQDDERKLMLKWEET+KELERKERHFNDWHYKYQQRRTPDELDPEKSEDN++DSVVTE
Sbjct: 601 MLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDSVVTE 660

Query: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR 720
           KLI VES KRRLEEEKETHAKQCLHVREKSLL+LKNQLPELFRALSEFSFSSSEMYKNLR
Sbjct: 661 KLIEVESWKRRLEEEKETHAKQCLHVREKSLLSLKNQLPELFRALSEFSFSSSEMYKNLR 720

Query: 721 SVCQV 726
           SVCQV
Sbjct: 721 SVCQV 725

BLAST of CmaCh01G001140 vs. NCBI nr
Match: KAG7036484.1 (hypothetical protein SDJN02_00101, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1348.2 bits (3488), Expect = 0.0e+00
Identity = 694/725 (95.72%), Postives = 709/725 (97.79%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAIARCKERKIHMKEAATARNAFAAAHSAYSMSLKNIGAALSDYAHGEV 60
           MGCSQSKIDNEEAIARCKERKIHMKEA T RNAFAAAHSAYSMSLKNIGAALSDYAHGEV
Sbjct: 1   MGCSQSKIDNEEAIARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAALSDYAHGEV 60

Query: 61  ENSQFVDVSTQPNSAITSAPAPIEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPG 120
           EN QFVDVSTQPNSAIT+APAP EPFPPPPPLPPSNFPNSLERAATMPEMN HKSDLKPG
Sbjct: 61  ENPQFVDVSTQPNSAITTAPAPSEPFPPPPPLPPSNFPNSLERAATMPEMNGHKSDLKPG 120

Query: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLAHASPQVPPPPPSEN 180
           SPIIE+ED+NENEGS G LRRRRSNKSKRDEGSSRNRNSELNDNLA+ASPQVPPPP SEN
Sbjct: 121 SPIIEKEDENENEGSAGALRRRRSNKSKRDEGSSRNRNSELNDNLANASPQVPPPPQSEN 180

Query: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINREEIDIKPKVVDSDDIDEQRRSVKA 240
           RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYIN+EEIDIKPKVVDSDDIDEQR+SVKA
Sbjct: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVVDSDDIDEQRKSVKA 240

Query: 241 ETVEPLLEEPVEPPPCLPAEPATSVSKSSKKTNQAGSMGSTEGKRMVKPNLNLLQIFIDI 300
           ET EPLLEEPVEPPP LPAEPAT+V+KSSKK NQAGSMGSTEGKRMVKPNLNLLQIFIDI
Sbjct: 241 ETAEPLLEEPVEPPPSLPAEPATAVAKSSKKANQAGSMGSTEGKRMVKPNLNLLQIFIDI 300

Query: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVIAWNRSFRGLPNMDD 360
           DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVI WNRSFRGLPNMDD
Sbjct: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMDD 360

Query: 361 GKDDFYPEEQETHATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVATLNRLKKRDSNVEA 420
           GKDD Y EEQETHATVLDKLLAWEKKL DEVKAGE+MKFEYQKKVATLNRLKKRDSNVEA
Sbjct: 361 GKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEA 420

Query: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHE 480
           LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHE
Sbjct: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHE 480

Query: 481 MQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540
           MQLKIVSALR MDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW
Sbjct: 481 MQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540

Query: 541 LKQNLVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLDRLPDEHLKTAIFTFGGVINTI 600
           LKQN+VPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQL+RLPDEHLKTAIFTFGGVINTI
Sbjct: 541 LKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVINTI 600

Query: 601 MLQQDDERKLMLKWEETKKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNSEDSVVTE 660
           MLQQDDERKLMLKWEET+KELERKERHFNDWHYKYQQRRTPDELDPEKSEDN++DSVVTE
Sbjct: 601 MLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDSVVTE 660

Query: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR 720
           KLIVVESLKRRLEEEKETHAKQCLHVREKSLL+LKNQLPELFRALSEFSFSSSEMYKNLR
Sbjct: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLSLKNQLPELFRALSEFSFSSSEMYKNLR 720

Query: 721 SVCQV 726
           SVCQV
Sbjct: 721 SVCQV 725

BLAST of CmaCh01G001140 vs. NCBI nr
Match: XP_022948532.1 (nitrate regulatory gene2 protein-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1347.0 bits (3485), Expect = 0.0e+00
Identity = 694/725 (95.72%), Postives = 709/725 (97.79%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAIARCKERKIHMKEAATARNAFAAAHSAYSMSLKNIGAALSDYAHGEV 60
           MGCSQSKIDNEEA+ARCKERKIHMKEA T RNAFAAAHSAYSMSLKNIGA LSDYAHGEV
Sbjct: 1   MGCSQSKIDNEEAVARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAVLSDYAHGEV 60

Query: 61  ENSQFVDVSTQPNSAITSAPAPIEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPG 120
           EN QFV VSTQPNSAITSAPAP EPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKP 
Sbjct: 61  ENPQFVYVSTQPNSAITSAPAPSEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPE 120

Query: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLAHASPQVPPPPPSEN 180
           SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLA+ASPQVPPPP SEN
Sbjct: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLANASPQVPPPPQSEN 180

Query: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINREEIDIKPKVVDSDDIDEQRRSVKA 240
           RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYIN+EEIDIKPKVVDSDDIDEQR+SVKA
Sbjct: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVVDSDDIDEQRKSVKA 240

Query: 241 ETVEPLLEEPVEPPPCLPAEPATSVSKSSKKTNQAGSMGSTEGKRMVKPNLNLLQIFIDI 300
           ET EPLLEEPVEPPP LPAEPAT+V+KSSKK NQAGSMGSTEGKRMVKP+LNLLQIFIDI
Sbjct: 241 ETAEPLLEEPVEPPPSLPAEPATAVAKSSKKANQAGSMGSTEGKRMVKPDLNLLQIFIDI 300

Query: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVIAWNRSFRGLPNMDD 360
           DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVI WNRSFRGLPNMDD
Sbjct: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMDD 360

Query: 361 GKDDFYPEEQETHATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVATLNRLKKRDSNVEA 420
           GKDD Y EEQETHATVLDKLLAWEKKL DEVKAGE+MKFEYQKKVATLNRLKKRDSNVEA
Sbjct: 361 GKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEA 420

Query: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHE 480
           LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMAT+WDTMRAHHE
Sbjct: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATVWDTMRAHHE 480

Query: 481 MQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540
           MQLKIVSALR MDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW
Sbjct: 481 MQLKIVSALRWMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540

Query: 541 LKQNLVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLDRLPDEHLKTAIFTFGGVINTI 600
           LKQN+VPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQL+RLPDEHLKTAIFTFGGVI+TI
Sbjct: 541 LKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVISTI 600

Query: 601 MLQQDDERKLMLKWEETKKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNSEDSVVTE 660
           MLQQDDERKLMLKWEET+KELERKERHFNDWHYKYQQRRTPDELDPEKSEDN++DSVVTE
Sbjct: 601 MLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDSVVTE 660

Query: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR 720
           KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR
Sbjct: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR 720

Query: 721 SVCQV 726
           SVCQV
Sbjct: 721 SVCQV 725

BLAST of CmaCh01G001140 vs. NCBI nr
Match: KAG6606771.1 (Protein ROLLING AND ERECT LEAF 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1341.3 bits (3470), Expect = 0.0e+00
Identity = 691/721 (95.84%), Postives = 705/721 (97.78%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAIARCKERKIHMKEAATARNAFAAAHSAYSMSLKNIGAALSDYAHGEV 60
           MGCSQSKIDNEEAIARCKERKIHMKEA T RNAFAAAHSAYSMSLKNIGAALSDYAHGEV
Sbjct: 1   MGCSQSKIDNEEAIARCKERKIHMKEAVTTRNAFAAAHSAYSMSLKNIGAALSDYAHGEV 60

Query: 61  ENSQFVDVSTQPNSAITSAPAPIEPFPPPPPLPPSNFPNSLERAATMPEMNVHKSDLKPG 120
           EN QFVDVSTQPNSAITSAPAP EPFPPPPPLPP NFPNSLERAATMPEMN HKSDLKPG
Sbjct: 61  ENPQFVDVSTQPNSAITSAPAPSEPFPPPPPLPPPNFPNSLERAATMPEMNGHKSDLKPG 120

Query: 121 SPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNLAHASPQVPPPPPSEN 180
           SPIIE+ED+NENEGS G LRRRRSNKSKRDEGSSRNRNSELNDNLA+ASPQVPPPP SEN
Sbjct: 121 SPIIEKEDENENEGSAGALRRRRSNKSKRDEGSSRNRNSELNDNLANASPQVPPPPQSEN 180

Query: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINREEIDIKPKVVDSDDIDEQRRSVKA 240
           RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYIN+EEIDIKPKVVDSDDIDEQR+SVKA
Sbjct: 181 RHIPPPPQQNSTYDYFFSMDLPVSTFSEVDEVYINKEEIDIKPKVVDSDDIDEQRKSVKA 240

Query: 241 ETVEPLLEEPVEPPPCLPAEPATSVSKSSKKTNQAGSMGSTEGKRMVKPNLNLLQIFIDI 300
           ET EPLLEEPVEPPP LPAEPAT+V+KSSKK NQAGSMGSTEGKRMVKPNLNLLQIFIDI
Sbjct: 241 ETAEPLLEEPVEPPPTLPAEPATAVAKSSKKANQAGSMGSTEGKRMVKPNLNLLQIFIDI 300

Query: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVIAWNRSFRGLPNMDD 360
           DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVI WNRSFRGLPNMDD
Sbjct: 301 DDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGLPNMDD 360

Query: 361 GKDDFYPEEQETHATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVATLNRLKKRDSNVEA 420
           GKDD Y EEQETHATVLDKLLAWEKKL DEVKAGE+MKFEYQKKVATLNRLKKRDSNVEA
Sbjct: 361 GKDDSYAEEQETHATVLDKLLAWEKKLYDEVKAGEVMKFEYQKKVATLNRLKKRDSNVEA 420

Query: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHE 480
           LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHE
Sbjct: 421 LEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHE 480

Query: 481 MQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540
           MQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW
Sbjct: 481 MQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSW 540

Query: 541 LKQNLVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLDRLPDEHLKTAIFTFGGVINTI 600
           LKQN+VPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQL+RLPDEHLKTAIFTFGGVINTI
Sbjct: 541 LKQNVVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLERLPDEHLKTAIFTFGGVINTI 600

Query: 601 MLQQDDERKLMLKWEETKKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNSEDSVVTE 660
           MLQQDDERKLMLKWEET+KELERKERHFNDWHYKYQQRRTPDELDPEKSEDN++DSVVTE
Sbjct: 601 MLQQDDERKLMLKWEETEKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNTQDSVVTE 660

Query: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLR 720
           KLIVVESLKRRLEEEKETHAKQCLHVREKSLL+LKNQLPELFRALSEFSFSSSEMYKNLR
Sbjct: 661 KLIVVESLKRRLEEEKETHAKQCLHVREKSLLSLKNQLPELFRALSEFSFSSSEMYKNLR 720

Query: 721 S 722
           S
Sbjct: 721 S 721

BLAST of CmaCh01G001140 vs. TAIR 10
Match: AT1G52320.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF630 (InterPro:IPR006868), Protein of unknown function DUF632 (InterPro:IPR006867); BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF630 and DUF632) (TAIR:AT5G25590.1); Has 8725 Blast hits to 7476 proteins in 620 species: Archae - 10; Bacteria - 622; Metazoa - 3286; Fungi - 1319; Plants - 1442; Viruses - 221; Other Eukaryotes - 1825 (source: NCBI BLink). )

HSP 1 Score: 661.0 bits (1704), Expect = 1.1e-189
Identity = 402/779 (51.60%), Postives = 513/779 (65.85%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAIARCKERKIHMKEAATARNAFAAAHSAYSMSLKNIGAALSDYAHGE- 60
           MGC+QSKI+NEEA+ RCKERK  MK+A TARNAFAAAHSAY+M+LKN GAALSDY+HGE 
Sbjct: 1   MGCAQSKIENEEAVTRCKERKQLMKDAVTARNAFAAAHSAYAMALKNTGAALSDYSHGEF 60

Query: 61  -VEN--------------------SQFVDVSTQP------NSAITSAPAPIEPFPPPPPL 120
            V N                    S  +  ST P      +S+  + P PI    PPPP 
Sbjct: 61  LVSNHSSSSAAAAIASTSSLPTAISPPLPSSTAPVSNSTASSSSAAVPQPIPDTLPPPPP 120

Query: 121 PPSNFPNSLERAATMPEMNVHKSDLKPGSPI--IEE--------EDKNENEGSVGGLRRR 180
           PP   P  L+RAATMPEMN        GS +  IEE        +D ++++ S    R R
Sbjct: 121 PP---PLPLQRAATMPEMNGRSGGGHAGSGLNGIEEDGALDNDDDDDDDDDDSEMENRDR 180

Query: 181 RSNKSKRDEGSSRNRNSELNDNLAHASPQVPPPPPSENRHIPPP---------PQQNSTY 240
              KS+   GS+R   + + D+        PPPP + +R IPPP          QQ   Y
Sbjct: 181 LIRKSRSRGGSTRGNRTTIEDHHLQEEKAPPPPPLANSRPIPPPRQHQHQHQQQQQQPFY 240

Query: 241 DYFFS--MDLPVSTFSEVDEVYINREEIDIKPK----VVDSDDIDEQRRSVKAETVE--- 300
           DYFF    ++P +T  +       +    + P+    VV  DD DE+    + E  E   
Sbjct: 241 DYFFPNVENMPGTTLEDTPPQPQPQPTRPVPPQPHSPVVTEDDEDEEEEEEEEEEEEETV 300

Query: 301 ----PLLEEPVE--PPPCLPAEPATSVSKSSKKTNQAGSMGSTEGKRMVKPNLNLLQIFI 360
               PL+EE  +      +  E  T++ +  KK+   G  G   G RM     +L  +FI
Sbjct: 301 IERKPLVEERPKRVEEVTIELEKVTNL-RGMKKSKGIGIPGERRGMRMPVTATHLANVFI 360

Query: 361 DIDDHFLKSCESAREVSKMLEATRLHYHSNFADNRGHIDHSARVMRVIAWNRSFRGLPNM 420
           ++DD+FLK+ ESA +VSKMLEATRLHYHSNFADNRGHIDHSARVMRVI WNRSFRG+PN 
Sbjct: 361 ELDDNFLKASESAHDVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGIPNA 420

Query: 421 DDGKDDFYPEEQETHATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVATLNRLKKRDSNV 480
           DDGKDD   EE ETHATVLDKLLAWEKKL DEVKAGELMK EYQKKVA LNR+KKR  + 
Sbjct: 421 DDGKDDVDLEENETHATVLDKLLAWEKKLYDEVKAGELMKIEYQKKVAHLNRVKKRGGHS 480

Query: 481 EALEKAKAAVSHLHTRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAH 540
           ++LE+AKAAVSHLHTRYIVDMQS+DSTVSEI+RLRDEQLY KLV LV  M  MW+ M+ H
Sbjct: 481 DSLERAKAAVSHLHTRYIVDMQSMDSTVSEINRLRDEQLYLKLVHLVEAMGKMWEMMQIH 540

Query: 541 HEMQLKIVSALRSMDLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALN 600
           H+ Q +I   LRS+D+SQ+ KET+ HH+ERT+QL  VV++WH+QF +++  QK+YIKAL 
Sbjct: 541 HQRQAEISKVLRSLDVSQAVKETNDHHHERTIQLLAVVQEWHTQFCRMIDHQKEYIKALG 600

Query: 601 SWLKQNLVPIESSLKEKVSSPPRAQSPPIHKLLLAWDDQLDRLPDEHLKTAIFTFGGVIN 660
            WLK NL+PIES+LKEKVSSPPR  +P I KLL AW D+LD++PDE  K+AI  F  V++
Sbjct: 601 GWLKLNLIPIESTLKEKVSSPPRVPNPAIQKLLHAWYDRLDKIPDEMAKSAIINFAAVVS 660

Query: 661 TIMLQQDDERKLMLKWEETKKELERKERHFNDWHYKYQQRRTPDELDPEKSEDNSEDSVV 718
           TIM QQ+DE  L  K EET+KEL RK R F DW++KY Q+R P+ ++P++++++  D V 
Sbjct: 661 TIMQQQEDEISLRNKCEETRKELGRKIRQFEDWYHKYIQKRGPEGMNPDEADNDHNDEVA 720

BLAST of CmaCh01G001140 vs. TAIR 10
Match: AT5G25590.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 595.9 bits (1535), Expect = 4.4e-170
Identity = 366/774 (47.29%), Postives = 476/774 (61.50%), Query Frame = 0

Query: 1   MGCSQSKIDNEEAIARCKERKIHMKEAATARNAFAAAHSAYSMSLKNIGAALSDYAHGEV 60
           MGC+QS++DNEEA+ARCKER+  +KEA +A  AFAA H AY+++LKN GAALSDY HGE 
Sbjct: 1   MGCAQSRVDNEEAVARCKERRNVIKEAVSASKAFAAGHFAYAIALKNTGAALSDYGHGES 60

Query: 61  ENSQFVDV-------------STQPNSAITSAPAPIEPFPPPPPLPPSNFPNSLERAATM 120
           +     DV             +  P S     P PIE  PPPPP  P   P+ ++RA ++
Sbjct: 61  DQKALDDVLLDQQHYEKQSRNNVDPASPQPPPPPPIENLPPPPPPLPKFSPSPIKRAISL 120

Query: 121 PEMNV--HKSDLKPGSPIIEEEDKNENEGSVGGLRRRRSNKSKRDEGSSRNRNSELNDNL 180
           P M V   K     G  I EEE+  E E  V G  R  + + +          S     L
Sbjct: 121 PSMAVRGRKVQTLDGMAIEEEEEDEEEEEEVKGSGRDTAQEEEEPRTPENVGKSNGRKRL 180

Query: 181 AHASPQVPPPPPSENRHIPPPPQQNSTYDYFF---SMDLPVSTFSEVDEVYINREEIDIK 240
              +P++          +   P  +  +DYFF   +M  P     EV   Y N+      
Sbjct: 181 EKTTPEI----------VSASPANSMAWDYFFMVENMPGPNLDDREVRNGYENQSSHFQF 240

Query: 241 PKVVDSDDIDEQRRSV---------KAETVEPLLEEPVEPPPCLPAEP------------ 300
            +  D ++ +E+R  +           E +EP   E VE       E             
Sbjct: 241 NEEDDEEEEEEERSGIYRKKSGSGKVVEEMEPKTPEKVEEEEEEDEEEDEEEEEEEEEEV 300

Query: 301 --ATSVSKSSKKTNQAGSMGSTEGKRMV-------KPNLNLLQIFIDIDDHFLKSCESAR 360
                  K  K   +  S    E +R V         ++NL++I  +IDD FLK+ E A+
Sbjct: 301 VVEVKKKKKGKAKIEHSSTAPPEFRRAVAKTSAAASSSVNLMKILDEIDDRFLKASECAQ 360

Query: 361 EVSKMLEATRLHYHSNFADNRGHIDHSARVMRVIAWNRSFRGLPNMDDGKDDFYPEEQET 420
           EVSKMLEATRLHYHSNFADNRG++DHSARVMRVI WN+S RG+ N + GKDD   +E ET
Sbjct: 361 EVSKMLEATRLHYHSNFADNRGYVDHSARVMRVITWNKSLRGISNGEGGKDDQESDEHET 420

Query: 421 HATVLDKLLAWEKKLSDEVKAGELMKFEYQKKVATLNRLKKRDSNVEALEKAKAAVSHLH 480
           HATVLDKLLAWEKKL DEVK GELMK EYQKKV+ LNR KKR ++ E +EK KAAVSHLH
Sbjct: 421 HATVLDKLLAWEKKLYDEVKQGELMKIEYQKKVSLLNRHKKRGASAETVEKTKAAVSHLH 480

Query: 481 TRYIVDMQSLDSTVSEISRLRDEQLYPKLVQLVNGMATMWDTMRAHHEMQLKIVSALRSM 540
           TRYIVDMQS+DSTVSE++RLRD+QLYP+LV LV GMA MW  M  HH+ QL IV  L+++
Sbjct: 481 TRYIVDMQSMDSTVSEVNRLRDDQLYPRLVALVEGMAKMWTNMCIHHDTQLGIVGELKAL 540

Query: 541 DLSQSPKETSTHHYERTVQLCGVVRDWHSQFEKLVRCQKDYIKALNSWLKQNLVPIESSL 600
           ++S S KET+  H+ +T Q C V+ +WH QF+ LV  QK YI +LN+WLK NL+PIESSL
Sbjct: 541 EISTSLKETTKQHHHQTRQFCTVLEEWHVQFDTLVTHQKQYINSLNNWLKLNLIPIESSL 600

Query: 601 KEKVSSPPRAQSPPIHKLLLAWDDQLDRLPDEHLKTAIFTFGGVINTIMLQQDDERKLML 660
           KEKVSSPPR Q PPI  LL +W D+L++LPDE  K+AI +F  VI TI+L Q++E KL  
Sbjct: 601 KEKVSSPPRPQRPPIQALLHSWHDRLEKLPDEVAKSAISSFAAVIKTILLHQEEEMKLKE 660

Query: 661 KWEETKKELERKERHFNDWHYKYQQRRTPDELDPEKSED--NSEDSVVTEKLIVVESLKR 720
           K EET++E  RK++ F DW+ K+ Q+R P E + E  +D   S    VTE+ I VE+LK+
Sbjct: 661 KCEETRREFIRKKQGFEDWYQKHLQKRGPTE-EAEGGDDATTSSRDHVTERRIAVETLKK 720

Query: 721 RLEEEKETHAKQCLHVREKSLLNLKNQLPELFRALSEFSFSSSEMYKNLRSVCQ 725
           RLEEE+E H + C+ VREKSL +LK +LPE+FRALS+++ + ++ Y+ LR + Q
Sbjct: 721 RLEEEEEAHQRHCVQVREKSLNSLKIRLPEIFRALSDYAHACADSYEKLRIISQ 763

BLAST of CmaCh01G001140 vs. TAIR 10
Match: AT1G52320.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF632 (InterPro:IPR006867); BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF630 and DUF632) (TAIR:AT5G25590.1); Has 517 Blast hits to 513 proteins in 62 species: Archae - 6; Bacteria - 6; Metazoa - 50; Fungi - 2; Plants - 427; Viruses - 0; Other Eukaryotes - 26 (source: NCBI BLink). )

HSP 1 Score: 549.7 bits (1415), Expect = 3.6e-156
Identity = 280/448 (62.50%), Postives = 351/448 (78.35%), Query Frame = 0

Query: 270 KKTNQAGSMGSTEGKRMVKPNLNLLQIFIDIDDHFLKSCESAREVSKMLEATRLHYHSNF 329
           KK+   G  G   G RM     +L  +FI++DD+FLK+ ESA +VSKMLEATRLHYHSNF
Sbjct: 2   KKSKGIGIPGERRGMRMPVTATHLANVFIELDDNFLKASESAHDVSKMLEATRLHYHSNF 61

Query: 330 ADNRGHIDHSARVMRVIAWNRSFRGLPNMDDGKDDFYPEEQETHATVLDKLLAWEKKLSD 389
           ADNRGHIDHSARVMRVI WNRSFRG+PN DDGKDD   EE ETHATVLDKLLAWEKKL D
Sbjct: 62  ADNRGHIDHSARVMRVITWNRSFRGIPNADDGKDDVDLEENETHATVLDKLLAWEKKLYD 121

Query: 390 EVKAGELMKFEYQKKVATLNRLKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEI 449
           EVKAGELMK EYQKKVA LNR+KKR  + ++LE+AKAAVSHLHTRYIVDMQS+DSTVSEI
Sbjct: 122 EVKAGELMKIEYQKKVAHLNRVKKRGGHSDSLERAKAAVSHLHTRYIVDMQSMDSTVSEI 181

Query: 450 SRLRDEQLYPKLVQLVNGMATMWDTMRAHHEMQLKIVSALRSMDLSQSPKETSTHHYERT 509
           +RLRDEQLY KLV LV  M  MW+ M+ HH+ Q +I   LRS+D+SQ+ KET+ HH+ERT
Sbjct: 182 NRLRDEQLYLKLVHLVEAMGKMWEMMQIHHQRQAEISKVLRSLDVSQAVKETNDHHHERT 241

Query: 510 VQLCGVVRDWHSQFEKLVRCQKDYIKALNSWLKQNLVPIESSLKEKVSSPPRAQSPPIHK 569
           +QL  VV++WH+QF +++  QK+YIKAL  WLK NL+PIES+LKEKVSSPPR  +P I K
Sbjct: 242 IQLLAVVQEWHTQFCRMIDHQKEYIKALGGWLKLNLIPIESTLKEKVSSPPRVPNPAIQK 301

Query: 570 LLLAWDDQLDRLPDEHLKTAIFTFGGVINTIMLQQDDERKLMLKWEETKKELERKERHFN 629
           LL AW D+LD++PDE  K+AI  F  V++TIM QQ+DE  L  K EET+KEL RK R F 
Sbjct: 302 LLHAWYDRLDKIPDEMAKSAIINFAAVVSTIMQQQEDEISLRNKCEETRKELGRKIRQFE 361

Query: 630 DWHYKYQQRRTPDELDPEKSEDNSEDSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREK 689
           DW++KY Q+R P+ ++P++++++  D V   +   VE +K+RLEEE+E + +Q   VREK
Sbjct: 362 DWYHKYIQKRGPEGMNPDEADNDHNDEVAVRQ-FNVEQIKKRLEEEEEAYHRQSHQVREK 421

Query: 690 SLLNLKNQLPELFRALSEFSFSSSEMYK 718
           SL +L+ +LPELF+A+SE ++S S+MY+
Sbjct: 422 SLASLRTRLPELFQAMSEVAYSCSDMYR 448

BLAST of CmaCh01G001140 vs. TAIR 10
Match: AT1G52320.3 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF632 (InterPro:IPR006867); BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF630 and DUF632) (TAIR:AT5G25590.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 549.7 bits (1415), Expect = 3.6e-156
Identity = 280/448 (62.50%), Postives = 351/448 (78.35%), Query Frame = 0

Query: 270 KKTNQAGSMGSTEGKRMVKPNLNLLQIFIDIDDHFLKSCESAREVSKMLEATRLHYHSNF 329
           KK+   G  G   G RM     +L  +FI++DD+FLK+ ESA +VSKMLEATRLHYHSNF
Sbjct: 2   KKSKGIGIPGERRGMRMPVTATHLANVFIELDDNFLKASESAHDVSKMLEATRLHYHSNF 61

Query: 330 ADNRGHIDHSARVMRVIAWNRSFRGLPNMDDGKDDFYPEEQETHATVLDKLLAWEKKLSD 389
           ADNRGHIDHSARVMRVI WNRSFRG+PN DDGKDD   EE ETHATVLDKLLAWEKKL D
Sbjct: 62  ADNRGHIDHSARVMRVITWNRSFRGIPNADDGKDDVDLEENETHATVLDKLLAWEKKLYD 121

Query: 390 EVKAGELMKFEYQKKVATLNRLKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEI 449
           EVKAGELMK EYQKKVA LNR+KKR  + ++LE+AKAAVSHLHTRYIVDMQS+DSTVSEI
Sbjct: 122 EVKAGELMKIEYQKKVAHLNRVKKRGGHSDSLERAKAAVSHLHTRYIVDMQSMDSTVSEI 181

Query: 450 SRLRDEQLYPKLVQLVNGMATMWDTMRAHHEMQLKIVSALRSMDLSQSPKETSTHHYERT 509
           +RLRDEQLY KLV LV  M  MW+ M+ HH+ Q +I   LRS+D+SQ+ KET+ HH+ERT
Sbjct: 182 NRLRDEQLYLKLVHLVEAMGKMWEMMQIHHQRQAEISKVLRSLDVSQAVKETNDHHHERT 241

Query: 510 VQLCGVVRDWHSQFEKLVRCQKDYIKALNSWLKQNLVPIESSLKEKVSSPPRAQSPPIHK 569
           +QL  VV++WH+QF +++  QK+YIKAL  WLK NL+PIES+LKEKVSSPPR  +P I K
Sbjct: 242 IQLLAVVQEWHTQFCRMIDHQKEYIKALGGWLKLNLIPIESTLKEKVSSPPRVPNPAIQK 301

Query: 570 LLLAWDDQLDRLPDEHLKTAIFTFGGVINTIMLQQDDERKLMLKWEETKKELERKERHFN 629
           LL AW D+LD++PDE  K+AI  F  V++TIM QQ+DE  L  K EET+KEL RK R F 
Sbjct: 302 LLHAWYDRLDKIPDEMAKSAIINFAAVVSTIMQQQEDEISLRNKCEETRKELGRKIRQFE 361

Query: 630 DWHYKYQQRRTPDELDPEKSEDNSEDSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREK 689
           DW++KY Q+R P+ ++P++++++  D V   +   VE +K+RLEEE+E + +Q   VREK
Sbjct: 362 DWYHKYIQKRGPEGMNPDEADNDHNDEVAVRQ-FNVEQIKKRLEEEEEAYHRQSHQVREK 421

Query: 690 SLLNLKNQLPELFRALSEFSFSSSEMYK 718
           SL +L+ +LPELF+A+SE ++S S+MY+
Sbjct: 422 SLASLRTRLPELFQAMSEVAYSCSDMYR 448

BLAST of CmaCh01G001140 vs. TAIR 10
Match: AT1G52320.4 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF632 (InterPro:IPR006867); BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF630 and DUF632) (TAIR:AT5G25590.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 549.7 bits (1415), Expect = 3.6e-156
Identity = 280/448 (62.50%), Postives = 351/448 (78.35%), Query Frame = 0

Query: 270 KKTNQAGSMGSTEGKRMVKPNLNLLQIFIDIDDHFLKSCESAREVSKMLEATRLHYHSNF 329
           KK+   G  G   G RM     +L  +FI++DD+FLK+ ESA +VSKMLEATRLHYHSNF
Sbjct: 2   KKSKGIGIPGERRGMRMPVTATHLANVFIELDDNFLKASESAHDVSKMLEATRLHYHSNF 61

Query: 330 ADNRGHIDHSARVMRVIAWNRSFRGLPNMDDGKDDFYPEEQETHATVLDKLLAWEKKLSD 389
           ADNRGHIDHSARVMRVI WNRSFRG+PN DDGKDD   EE ETHATVLDKLLAWEKKL D
Sbjct: 62  ADNRGHIDHSARVMRVITWNRSFRGIPNADDGKDDVDLEENETHATVLDKLLAWEKKLYD 121

Query: 390 EVKAGELMKFEYQKKVATLNRLKKRDSNVEALEKAKAAVSHLHTRYIVDMQSLDSTVSEI 449
           EVKAGELMK EYQKKVA LNR+KKR  + ++LE+AKAAVSHLHTRYIVDMQS+DSTVSEI
Sbjct: 122 EVKAGELMKIEYQKKVAHLNRVKKRGGHSDSLERAKAAVSHLHTRYIVDMQSMDSTVSEI 181

Query: 450 SRLRDEQLYPKLVQLVNGMATMWDTMRAHHEMQLKIVSALRSMDLSQSPKETSTHHYERT 509
           +RLRDEQLY KLV LV  M  MW+ M+ HH+ Q +I   LRS+D+SQ+ KET+ HH+ERT
Sbjct: 182 NRLRDEQLYLKLVHLVEAMGKMWEMMQIHHQRQAEISKVLRSLDVSQAVKETNDHHHERT 241

Query: 510 VQLCGVVRDWHSQFEKLVRCQKDYIKALNSWLKQNLVPIESSLKEKVSSPPRAQSPPIHK 569
           +QL  VV++WH+QF +++  QK+YIKAL  WLK NL+PIES+LKEKVSSPPR  +P I K
Sbjct: 242 IQLLAVVQEWHTQFCRMIDHQKEYIKALGGWLKLNLIPIESTLKEKVSSPPRVPNPAIQK 301

Query: 570 LLLAWDDQLDRLPDEHLKTAIFTFGGVINTIMLQQDDERKLMLKWEETKKELERKERHFN 629
           LL AW D+LD++PDE  K+AI  F  V++TIM QQ+DE  L  K EET+KEL RK R F 
Sbjct: 302 LLHAWYDRLDKIPDEMAKSAIINFAAVVSTIMQQQEDEISLRNKCEETRKELGRKIRQFE 361

Query: 630 DWHYKYQQRRTPDELDPEKSEDNSEDSVVTEKLIVVESLKRRLEEEKETHAKQCLHVREK 689
           DW++KY Q+R P+ ++P++++++  D V   +   VE +K+RLEEE+E + +Q   VREK
Sbjct: 362 DWYHKYIQKRGPEGMNPDEADNDHNDEVAVRQ-FNVEQIKKRLEEEEEAYHRQSHQVREK 421

Query: 690 SLLNLKNQLPELFRALSEFSFSSSEMYK 718
           SL +L+ +LPELF+A+SE ++S S+MY+
Sbjct: 422 SLASLRTRLPELFQAMSEVAYSCSDMYR 448

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9AQW13.7e-5729.60Protein ROLLING AND ERECT LEAF 2 OS=Oryza sativa subsp. japonica OX=39947 GN=REL... [more]
A0A178VBJ02.0e-5526.95Protein ALTERED PHOSPHATE STARVATION RESPONSE 1 OS=Arabidopsis thaliana OX=3702 ... [more]
Q93YU88.6e-5427.72Nitrate regulatory gene2 protein OS=Arabidopsis thaliana OX=3702 GN=NRG2 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1K8S00.0e+00100.00nitrate regulatory gene2 protein-like isoform X1 OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1G9I10.0e+0095.72nitrate regulatory gene2 protein-like isoform X1 OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1KHK40.0e+00100.00nitrate regulatory gene2 protein-like isoform X2 OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1G9J80.0e+0096.12nitrate regulatory gene2 protein-like isoform X2 OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1ISF00.0e+0082.31nitrate regulatory gene2 protein-like OS=Cucurbita maxima OX=3661 GN=LOC11148012... [more]
Match NameE-valueIdentityDescription
XP_022998727.10.0e+00100.00nitrate regulatory gene2 protein-like isoform X1 [Cucurbita maxima][more]
XP_023524816.10.0e+0096.41nitrate regulatory gene2 protein-like isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG7036484.10.0e+0095.72hypothetical protein SDJN02_00101, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022948532.10.0e+0095.72nitrate regulatory gene2 protein-like isoform X1 [Cucurbita moschata][more]
KAG6606771.10.0e+0095.84Protein ROLLING AND ERECT LEAF 2, partial [Cucurbita argyrosperma subsp. sororia... [more]
Match NameE-valueIdentityDescription
AT1G52320.21.1e-18951.60unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-termin... [more]
AT5G25590.14.4e-17047.29Protein of unknown function (DUF630 and DUF632) [more]
AT1G52320.13.6e-15662.50unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-termin... [more]
AT1G52320.33.6e-15662.50unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-termin... [more]
AT1G52320.43.6e-15662.50unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-termin... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 669..689
NoneNo IPR availableCOILSCoilCoilcoord: 604..624
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 169..184
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 262..280
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 119..157
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 227..246
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 81..97
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 71..194
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 227..284
NoneNo IPR availablePANTHERPTHR21450UNCHARACTERIZEDcoord: 1..724
NoneNo IPR availablePANTHERPTHR21450:SF33PROTEIN, PUTATIVE, 48652-45869-RELATEDcoord: 1..724
IPR006867Domain of unknown function DUF632PFAMPF04782DUF632coord: 293..598
e-value: 9.1E-99
score: 330.8
IPR006868Domain of unknown function DUF630PFAMPF04783DUF630coord: 1..59
e-value: 1.0E-23
score: 83.3

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G001140.1CmaCh01G001140.1mRNA