CmaCh01G000450 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh01G000450
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionprotein BIG GRAIN 1-like B
LocationCma_Chr01: 166101 .. 167192 (-)
RNA-Seq ExpressionCmaCh01G000450
SyntenyCmaCh01G000450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTTGTGGGATAAGTCGTTTCAGAGACACGATCAAGACCTCCCACAACGCCGTCGTAGTCGCCGTGACAACCCTTCCTTCTCCTCCTCTCTTCTCGACGCCATCTACCGTTCCATCGACGAATCCAACCACCAGGATGAAGACGGCCACCTCTTTTTTTACAGAGAAACCACCATGAGAACAAAACAGAGTGTGGTTACCGCCAGGACAGAGGACGACGAAGCCGTCAAATTCCGGCGAGCTTGTATGATCGAGAAGTGGATGGATAGGAAAAAAGTTAGAAACATTCGCGATTTTACTGTTTCCAGCTCCAGCTCGTCGGAATCCACCTCCACCGCCGGCAGAAGATACTCTGCGTCGGAATCGGAGCTTCTGTCACGTGCTCTGCACCGACAAAAGCCGATCAAAACTACTCCGTGGACTCAAACCGAAACCCAAAAGGCAAAGCACGAAAATGGGTTGGTTAAAACGAAATCCAAAGCTTCAAAAATCTACCACGATTTGAAAAAGGTGAAGCAGCCGATATCCCCCGGCGGCCGCCTCGCAAATTTTCTCAACTCCCTCTTCAATGGCGGAAGCCCAAAAACGAAGCAAAAGATGCCAAATCCTTCGACGAATCCAACGAGTATGGATTACGACATGCCGACAAAAGCGAAATCCCAGGGATCGACATGTTCCTCTGCTTCGTCATTTTCGAGGTCTTGTCTGAGCAAAACGCCATCTTCGAGAGGAAACGTTAAGAGATCGGTTCGGTTCTGTCCTGTGAGTGAAATTGTGGACGAGGATTGTGAGGCATATGGGCACAAAAGTTTGCAGAAGCTAGAGGAACCCATTATGAAGAAAGGGTTGAAAAGTTATGGGAATTTTGAAACAGAGGAAGTGAAAACAGAGCATCCGAGGAAGATTAAGAGAAATGAAGGGTTTGAGTACGAGGATGAAGATGATGCAGTGAGCTGTTCGAGTTCTGATCTGTTTGAATTGGATAATCTTTCAGTCATTGGAATCGAAAGGTTCAGAGAGGAATTGCCAGTTTATGAAACTACAAATTTCAAAACAAATTGTGCCATTGCCAAGGGATTGCTTGTGTAG

mRNA sequence

ATGGATTTGTGGGATAAGTCGTTTCAGAGACACGATCAAGACCTCCCACAACGCCGTCGTAGTCGCCGTGACAACCCTTCCTTCTCCTCCTCTCTTCTCGACGCCATCTACCGTTCCATCGACGAATCCAACCACCAGGATGAAGACGGCCACCTCTTTTTTTACAGAGAAACCACCATGAGAACAAAACAGAGTGTGGTTACCGCCAGGACAGAGGACGACGAAGCCGTCAAATTCCGGCGAGCTTGTATGATCGAGAAGTGGATGGATAGGAAAAAAGTTAGAAACATTCGCGATTTTACTGTTTCCAGCTCCAGCTCGTCGGAATCCACCTCCACCGCCGGCAGAAGATACTCTGCGTCGGAATCGGAGCTTCTGTCACGTGCTCTGCACCGACAAAAGCCGATCAAAACTACTCCGTGGACTCAAACCGAAACCCAAAAGGCAAAGCACGAAAATGGGTTGGTTAAAACGAAATCCAAAGCTTCAAAAATCTACCACGATTTGAAAAAGGTGAAGCAGCCGATATCCCCCGGCGGCCGCCTCGCAAATTTTCTCAACTCCCTCTTCAATGGCGGAAGCCCAAAAACGAAGCAAAAGATGCCAAATCCTTCGACGAATCCAACGAGTATGGATTACGACATGCCGACAAAAGCGAAATCCCAGGGATCGACATGTTCCTCTGCTTCGTCATTTTCGAGGTCTTGTCTGAGCAAAACGCCATCTTCGAGAGGAAACGTTAAGAGATCGGTTCGGTTCTGTCCTGTGAGTGAAATTGTGGACGAGGATTGTGAGGCATATGGGCACAAAAGTTTGCAGAAGCTAGAGGAACCCATTATGAAGAAAGGGTTGAAAAGTTATGGGAATTTTGAAACAGAGGAAGTGAAAACAGAGCATCCGAGGAAGATTAAGAGAAATGAAGGGTTTGAGTACGAGGATGAAGATGATGCAGTGAGCTGTTCGAGTTCTGATCTGTTTGAATTGGATAATCTTTCAGTCATTGGAATCGAAAGGTTCAGAGAGGAATTGCCAGTTTATGAAACTACAAATTTCAAAACAAATTGTGCCATTGCCAAGGGATTGCTTGTGTAG

Coding sequence (CDS)

ATGGATTTGTGGGATAAGTCGTTTCAGAGACACGATCAAGACCTCCCACAACGCCGTCGTAGTCGCCGTGACAACCCTTCCTTCTCCTCCTCTCTTCTCGACGCCATCTACCGTTCCATCGACGAATCCAACCACCAGGATGAAGACGGCCACCTCTTTTTTTACAGAGAAACCACCATGAGAACAAAACAGAGTGTGGTTACCGCCAGGACAGAGGACGACGAAGCCGTCAAATTCCGGCGAGCTTGTATGATCGAGAAGTGGATGGATAGGAAAAAAGTTAGAAACATTCGCGATTTTACTGTTTCCAGCTCCAGCTCGTCGGAATCCACCTCCACCGCCGGCAGAAGATACTCTGCGTCGGAATCGGAGCTTCTGTCACGTGCTCTGCACCGACAAAAGCCGATCAAAACTACTCCGTGGACTCAAACCGAAACCCAAAAGGCAAAGCACGAAAATGGGTTGGTTAAAACGAAATCCAAAGCTTCAAAAATCTACCACGATTTGAAAAAGGTGAAGCAGCCGATATCCCCCGGCGGCCGCCTCGCAAATTTTCTCAACTCCCTCTTCAATGGCGGAAGCCCAAAAACGAAGCAAAAGATGCCAAATCCTTCGACGAATCCAACGAGTATGGATTACGACATGCCGACAAAAGCGAAATCCCAGGGATCGACATGTTCCTCTGCTTCGTCATTTTCGAGGTCTTGTCTGAGCAAAACGCCATCTTCGAGAGGAAACGTTAAGAGATCGGTTCGGTTCTGTCCTGTGAGTGAAATTGTGGACGAGGATTGTGAGGCATATGGGCACAAAAGTTTGCAGAAGCTAGAGGAACCCATTATGAAGAAAGGGTTGAAAAGTTATGGGAATTTTGAAACAGAGGAAGTGAAAACAGAGCATCCGAGGAAGATTAAGAGAAATGAAGGGTTTGAGTACGAGGATGAAGATGATGCAGTGAGCTGTTCGAGTTCTGATCTGTTTGAATTGGATAATCTTTCAGTCATTGGAATCGAAAGGTTCAGAGAGGAATTGCCAGTTTATGAAACTACAAATTTCAAAACAAATTGTGCCATTGCCAAGGGATTGCTTGTGTAG

Protein sequence

MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRETTMRTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSASESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGGRLANFLNSLFNGGSPKTKQKMPNPSTNPTSMDYDMPTKAKSQGSTCSSASSFSRSCLSKTPSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGNFETEEVKTEHPRKIKRNEGFEYEDEDDAVSCSSSDLFELDNLSVIGIERFREELPVYETTNFKTNCAIAKGLLV
Homology
BLAST of CmaCh01G000450 vs. ExPASy Swiss-Prot
Match: Q9SLL2 (Protein BIG GRAIN 1-like B OS=Arabidopsis thaliana OX=3702 GN=At1g54200 PE=1 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 1.1e-52
Identity = 176/406 (43.35%), Postives = 216/406 (53.20%), Query Frame = 0

Query: 1   MDLWDKSFQRHDQDLPQRRRSRRD--NPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRET 60
           MD WD        D   R R+RRD  +PSFSS+LLD IYRSID+S+           R+T
Sbjct: 1   MDPWDT--YNIPLDHQHRLRNRRDHRHPSFSSTLLDQIYRSIDDSSTNSSS-----MRKT 60

Query: 61  TMRTKQSV-VTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRR 120
             + ++   V+A   DD    F R+         K ++ I       SSSS S S+    
Sbjct: 61  KHQNREDTRVSANRRDD----FNRS---------KNLKTIEPVFFKHSSSSSSDSSG--- 120

Query: 121 YSASESELLSR------ALHRQKPIKTT-------PWTQTETQKAKHENG-LVKTKSKAS 180
           +S+SES+   R      A+   KPI+TT       P         K E+G  +KTKSKA 
Sbjct: 121 FSSSESDYFYRRSKSSPAISHPKPIRTTVERFERSPQNHRPNSSNKQEHGSFLKTKSKAL 180

Query: 181 KIYHDLKKVKQPISPGGRLANFLNSLFNG-GSPKTKQKMPNPSTNPTSMDYDMPTKAKSQ 240
           KIY DLKKVKQPISPGGRLA FLNS+F G G+ K   K+    T+ T+        A S 
Sbjct: 181 KIYSDLKKVKQPISPGGRLATFLNSIFTGAGNTKKLNKINTTVTSTTA-----AAAAASS 240

Query: 241 GSTCSSASSFSRSCLSKTPSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKK 300
            +TCSSASSFSRSCLSKTPSS    KRSVRFCPV+ I DED   Y +K+           
Sbjct: 241 TTTCSSASSFSRSCLSKTPSSSEKSKRSVRFCPVNVIFDEDSSKYNNKN----------- 300

Query: 301 GLKSYGNFETE----------EVKTEHPRKI-----------KRN--------EGFEYED 360
             K YGN E E           V  E+ R I           K+N        E  E +D
Sbjct: 301 -NKVYGNNEREYESIRHTLENRVMEENRRVIEAAKELLRSYQKKNKEVIEVSVEDDEEDD 360

BLAST of CmaCh01G000450 vs. ExPASy Swiss-Prot
Match: Q9LVK2 (Protein BIG GRAIN 1-like A OS=Arabidopsis thaliana OX=3702 GN=At3g13980 PE=3 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 2.2e-50
Identity = 157/358 (43.85%), Postives = 199/358 (55.59%), Query Frame = 0

Query: 21  SRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRETTMRTKQSVVTARTEDDEAVKFR 80
           S   NPSFSS+LLD IYRSID+S+       +   +    +   S+   R  +   +  R
Sbjct: 12  SHHRNPSFSSTLLDQIYRSIDDSSPPPPLESIKKKKHHHQQRNASLHEDR--EISPIYHR 71

Query: 81  RACMIEKWMDRKKVRNIRDFTVSSS-----SSSESTSTAGRRYSASESELLSRALHRQKP 140
           R+   +    R+K   +R    SSS     SSSES S  GR  S++     SR   + KP
Sbjct: 72  RSIAADFERSRRKTDFLRHSNSSSSDSSGFSSSESDSFHGRSKSSASPPSSSR--QQPKP 131

Query: 141 IKTTPWTQTE-TQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGGRLANFLNSLFNGGS 200
           I+T+    +   QK K   G ++TKSKA KIY DLKKVKQPISPGGRLA FLNSLF   +
Sbjct: 132 IRTSSVDHSSAVQKPKELGGFLRTKSKALKIYSDLKKVKQPISPGGRLATFLNSLFTNAA 191

Query: 201 PKTKQKMPNPSTNPTSMDYDMPTKAKSQGSTCSSASSFSRSCLSKTPSSRGNVKRSVRFC 260
              K+   +  T   ++      +     STCSSASSFSRSCLSKTPSS G  KRSVRFC
Sbjct: 192 TNPKK---HKKTTTVAV-----VEEPHSSSTCSSASSFSRSCLSKTPSSSGKSKRSVRFC 251

Query: 261 PVSEIVDEDCE-----AYGHKSLQKLEEPIMKKGLKSYGNFETEEVKTEHPRK---IKRN 320
           PV+ I+DED       AY ++ L    E    +  +       + ++T H +        
Sbjct: 252 PVNVILDEDSSFTMPYAYNNERLYDNNEAKRVEEHRRVIQAAKDLLRTYHNKNKVTTTNI 311

Query: 321 EGFEYEDEDDAVSCSSSDLFELDNLSVIGIERFREELPVYETTNF-KTNCAIAKGLLV 364
              E +DEDDA SC+SSDLFEL+NLS IGIER+REELPVYETT     N  IA GL+V
Sbjct: 312 NNVEEDDEDDAASCASSDLFELENLSAIGIERYREELPVYETTRLDNMNRVIATGLIV 357

BLAST of CmaCh01G000450 vs. ExPASy Swiss-Prot
Match: Q9M2B3 (Protein BIG GRAIN 1-like C OS=Arabidopsis thaliana OX=3702 GN=At3g42800 PE=2 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 1.1e-33
Identity = 136/375 (36.27%), Postives = 189/375 (50.40%), Query Frame = 0

Query: 16  PQRRRSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRETTMRTKQSVVTARTEDDE 75
           PQR+R+    PSFSSS+LD++YRSIDES+    D  L       + +  S  +   +DD+
Sbjct: 4   PQRKRT----PSFSSSVLDSVYRSIDESDGLQSD--LKGSINENVSSSSSSPSPNKKDDK 63

Query: 76  AVKFRRACMIEK-WM-DRKKVRNIRDFTVSSSSSSESTSTAGRRYSASESELLSRALHRQ 135
               RRA M E+ W+  R           SS SSSE+ S   +R     +E   R+   +
Sbjct: 64  LTTLRRAIMDEEHWLYARSSTTTTNSSDSSSFSSSEAESYRTKRRLRKLAEQGKRSGDER 123

Query: 136 KPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGGRLANFLNSLFNGG 195
           +  K T         +K ++     K KA KI  +LK+ KQP+SPG RL +FLNS+F   
Sbjct: 124 QRTKRTVMDNDSRLFSKSDD---DKKPKAVKIIEELKRSKQPVSPGARLTSFLNSIFQSN 183

Query: 196 SPKTKQKMPNPSTNPTSMDYDMPTKAKSQGSTCSSASSFSRSCLSKTPSSRGN------- 255
           + K                     K  S G T    SS S+SC S+T +   N       
Sbjct: 184 AKK--------------------VKLCSVGKTTDVKSSSSKSCFSRTRNKTDNNNNNCKK 243

Query: 256 VKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPI----MKKGLKSYGNFETEEVKT-EHPR 315
           ++RS+RF PV   +D DC  Y  K + ++ +PI     KK +K       EE+KT +H  
Sbjct: 244 LERSIRFYPVRVTIDGDCRDYAQKHITRVRKPIPEFTAKKSVK-------EEIKTNDHHT 303

Query: 316 K---IKRNEGFE----------YEDEDDAVSCSSSDLFELDNLSVIGIERFREELPVYET 364
           +   I RN G +           E+E+DA S SSSDLFELD+   IG+ R+ +ELPVYET
Sbjct: 304 EFTCITRNIGLKDFVRSNKYEGKEEEEDAWSHSSSDLFELDSYR-IGMGRYLKELPVYET 341

BLAST of CmaCh01G000450 vs. ExPASy Swiss-Prot
Match: Q9LYH0 (Protein BIG GRAIN 1-like D OS=Arabidopsis thaliana OX=3702 GN=At5g12050 PE=2 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 3.6e-24
Identity = 133/389 (34.19%), Postives = 182/389 (46.79%), Query Frame = 0

Query: 20  RSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRETTMRTKQSVVTARTEDDEAVKF 79
           +S+R NPSFSS+LLD IY SID    + +    +     T   KQS+VT    D      
Sbjct: 3   KSKR-NPSFSSTLLDEIYNSIDPKTQKTQP---YVGSVNTTTKKQSIVTRSVPD------ 62

Query: 80  RRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSASESELL-------SRAL-- 139
                       +K+   R F  S SSSS+S S+    +S+S++EL        SR L  
Sbjct: 63  ------------RKIHRDR-FFGSVSSSSDSNSSI---FSSSDTELTHGKKTTSSRPLCF 122

Query: 140 --HRQKPIKTTPWTQTETQKA-----------------KHENGLVKTKSKAS-KIYHDLK 199
              + KP KT   T     +A                 +H+     T+++ S K   + K
Sbjct: 123 GPSKTKPRKTEDKTLFHQNRATRVYDDYDYASDVPKFNRHDENWENTRNRRSVKSSGNQK 182

Query: 200 KVKQPISPGGRLANFLNSLFNGGSPKTKQKMPNPS---------TNPTSMDYDMPTKAKS 259
           K K P SPGGR+ NFLNSLF+  S ++      P             TS DY   T    
Sbjct: 183 KPKTPASPGGRIVNFLNSLFSNNSKQSNAVKSYPRKTSYDDSAYVRKTSNDYHSST---- 242

Query: 260 QGSTCSSASSFSRSCLSK-TPSSRGNVKRSVRFCPVSEIV-------DEDCEAYGH--KS 319
             +TCSSASSFSRSC++K    S G +KRSVRF PV+ IV       +ED  + G+  KS
Sbjct: 243 --TTCSSASSFSRSCMNKGYEKSSGRIKRSVRFSPVNVIVPESFTSKEEDYFSNGNARKS 302

Query: 320 LQKLEEPIMKKGLKSYGNFETEEVKTEHPRKIKRNEGFE-YE------DEDDAVSCSSSD 350
           ++K  E   ++ ++        +    H   + +  GFE YE      D+DD  S SSSD
Sbjct: 303 VKKNVEDGGRRSVEEIAREFLRDYHKNHENSLVKTNGFEDYEDDDEDDDDDDVASDSSSD 356

BLAST of CmaCh01G000450 vs. ExPASy Swiss-Prot
Match: Q10R09 (Protein BIG GRAIN 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BG1 PE=1 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 1.5e-22
Identity = 118/349 (33.81%), Postives = 166/349 (47.56%), Query Frame = 0

Query: 19  RRSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRETTMRTKQSVVTARTEDDEAV- 78
           RR   D PSFSS+LLDAIY+S+DE     + GH            ++V  A  +  EA  
Sbjct: 15  RRYVADQPSFSSTLLDAIYKSMDE-----QPGH-----GGGATGVEAVAAAAKKQHEAAL 74

Query: 79  ---KFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSASESELLSRALHRQK 138
               + +  +   +  R    +      ++SSSSE +S  G  +S+SE+E  S    R +
Sbjct: 75  HYGNYYKPSLAGSYRARAPGPH-----ATTSSSSECSSYGG--FSSSEAE--SSHHRRLR 134

Query: 139 PIKTT-PWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGGRLANFLNSLFNGG 198
           PI+TT P         K      K K   + I   L+ +++P SPG RLA FLNS+F G 
Sbjct: 135 PIRTTVPGGAPGPAPEK------KAKKPGASIRAKLRDLRKPASPGARLAGFLNSIFAG- 194

Query: 199 SPKTKQKMPNPSTNPTSMDYDMPTKAKSQGSTCSSASSFSRSCLSKTPSSRGNVKRSVRF 258
               K+    P           P+      S CS+ASS+SRSCLSKTPS+RG  KR+VRF
Sbjct: 195 ----KRAPATP-----------PSATAGAESACSTASSYSRSCLSKTPSTRGQAKRTVRF 254

Query: 259 CPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGNFETEEVKTEHPRKIKRNEGFEYED 318
                 +D D E+    ++                +     V+      ++R E  E ++
Sbjct: 255 ------LDSDTESLASSTVV---------------DRRRVPVEAVQQMLLQRME-MESDE 300

Query: 319 EDDAVSCSSSDLFELDNLSVIGI--ERFREELPVYETTNFKTNCAIAKG 361
           +DD  S +SSDLFEL+N + I      +R+ELPVYETT    N AI  G
Sbjct: 315 DDDESSDASSDLFELENFAAIAPAGAAYRDELPVYETTRVALNRAIGHG 300

BLAST of CmaCh01G000450 vs. ExPASy TrEMBL
Match: A0A6J1KB46 (protein BIG GRAIN 1-like B OS=Cucurbita maxima OX=3661 GN=LOC111493335 PE=3 SV=1)

HSP 1 Score: 706.1 bits (1821), Expect = 7.8e-200
Identity = 363/363 (100.00%), Postives = 363/363 (100.00%), Query Frame = 0

Query: 1   MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRETTM 60
           MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRETTM
Sbjct: 84  MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRETTM 143

Query: 61  RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA 120
           RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA
Sbjct: 144 RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA 203

Query: 121 SESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGG 180
           SESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGG
Sbjct: 204 SESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGG 263

Query: 181 RLANFLNSLFNGGSPKTKQKMPNPSTNPTSMDYDMPTKAKSQGSTCSSASSFSRSCLSKT 240
           RLANFLNSLFNGGSPKTKQKMPNPSTNPTSMDYDMPTKAKSQGSTCSSASSFSRSCLSKT
Sbjct: 264 RLANFLNSLFNGGSPKTKQKMPNPSTNPTSMDYDMPTKAKSQGSTCSSASSFSRSCLSKT 323

Query: 241 PSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGNFETEEVKTEHP 300
           PSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGNFETEEVKTEHP
Sbjct: 324 PSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGNFETEEVKTEHP 383

Query: 301 RKIKRNEGFEYEDEDDAVSCSSSDLFELDNLSVIGIERFREELPVYETTNFKTNCAIAKG 360
           RKIKRNEGFEYEDEDDAVSCSSSDLFELDNLSVIGIERFREELPVYETTNFKTNCAIAKG
Sbjct: 384 RKIKRNEGFEYEDEDDAVSCSSSDLFELDNLSVIGIERFREELPVYETTNFKTNCAIAKG 443

Query: 361 LLV 364
           LLV
Sbjct: 444 LLV 446

BLAST of CmaCh01G000450 vs. ExPASy TrEMBL
Match: A0A6J1GAP7 (protein BIG GRAIN 1-like B OS=Cucurbita moschata OX=3662 GN=LOC111452438 PE=3 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 7.1e-193
Identity = 355/369 (96.21%), Postives = 358/369 (97.02%), Query Frame = 0

Query: 1   MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRETTM 60
           MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESN QDEDGHLFFYRETTM
Sbjct: 1   MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNDQDEDGHLFFYRETTM 60

Query: 61  RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA 120
           RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA
Sbjct: 61  RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA 120

Query: 121 SESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGG 180
           SESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGG
Sbjct: 121 SESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGG 180

Query: 181 RLANFLNSLFNGGSPKTKQKMPNPSTNPTSMDYDMPTKAKSQGSTCSSASSFSRSCLSKT 240
           RLANFLNSLFNGGSPKTKQKMPNPSTNP SMDYDMPTKAKSQGSTCSSASSFSRSCLSKT
Sbjct: 181 RLANFLNSLFNGGSPKTKQKMPNPSTNPRSMDYDMPTKAKSQGSTCSSASSFSRSCLSKT 240

Query: 241 PSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGNFETEEVKTEHP 300
           PSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYG FETEEVKTEHP
Sbjct: 241 PSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGKFETEEVKTEHP 300

Query: 301 RKIKRNEGFEYED------EDDAVSCSSSDLFELDNLSVIGIERFREELPVYETTNFKTN 360
           RK K NEGFEYED      EDDAVSCSSSDLFELDNLSVIG+ER+REELPVYETTNFKTN
Sbjct: 301 RKSKGNEGFEYEDEDEEEEEDDAVSCSSSDLFELDNLSVIGMERYREELPVYETTNFKTN 360

Query: 361 CAIAKGLLV 364
           CAIAKGL+V
Sbjct: 361 CAIAKGLVV 369

BLAST of CmaCh01G000450 vs. ExPASy TrEMBL
Match: A0A6J1IZW5 (protein BIG GRAIN 1-like B OS=Cucurbita maxima OX=3661 GN=LOC111479960 PE=3 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 9.1e-124
Identity = 256/379 (67.55%), Postives = 293/379 (77.31%), Query Frame = 0

Query: 1   MDLWDKSFQRHDQD---LPQRRRSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRE 60
           MDLWD SFQR D D     QRRRSRR+NPSFSSSLLDAIYRSID+SN Q ED  +F+ ++
Sbjct: 1   MDLWDNSFQREDPDPHHHHQRRRSRRENPSFSSSLLDAIYRSIDDSNVQGEDQLIFYTQK 60

Query: 61  TTMRTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRR 120
           TT RTKQS+  A+TED + ++F+RA MI+ WMD KK++ +RDFTVS+SSSSES+STAGRR
Sbjct: 61  TTTRTKQSISAAKTEDHQTLQFQRASMIKNWMDNKKIKTVRDFTVSTSSSSESSSTAGRR 120

Query: 121 YSASESELLSRALHR--------QKPIKTTPWTQTE-TQKAKHENGLVKTKSKASKIYHD 180
            S+SESE LSR LHR         KPIKTT W+QTE      HENG VK+K +ASKIYHD
Sbjct: 121 LSSSESEFLSRTLHRPKPKPKPKPKPIKTTTWSQTEIIPNPNHENGFVKSKFRASKIYHD 180

Query: 181 LKKVKQPISPGGRLANFLNSLFNGGSPKTKQKMPNP-STNPTSMDYDMPTKAKSQ---GS 240
           LKKVKQPISPGGRLANFLNSLFNGGSPK KQK+ N  S N T++DYD P K+KSQ    S
Sbjct: 181 LKKVKQPISPGGRLANFLNSLFNGGSPKAKQKISNTCSNNSTNLDYDAPRKSKSQQGSSS 240

Query: 241 TCSSASSFSRSCLSKTPSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGL 300
           TCSSASSFSRSCL KTPSSRGN+KRSVRFCPVS IVDEDC   GHK L KLEEPI KK  
Sbjct: 241 TCSSASSFSRSCLIKTPSSRGNIKRSVRFCPVSVIVDEDCRPCGHKMLHKLEEPIGKK-- 300

Query: 301 KSYGNFETEEVKTEHPRKIKRNEGFEYEDEDDAVSCSSSDLFELDNLSVIGIERFREELP 360
              G  ETEE+KTE           E +++DD +SCSSSDLFELDNLS IG+ER+R+ELP
Sbjct: 301 ---GKLETEEMKTE-----------EDDEDDDRLSCSSSDLFELDNLSAIGMERYRDELP 360

Query: 361 VYETTNFKTNCAIAKGLLV 364
           VYETT+F TNCAIA GLL+
Sbjct: 361 VYETTHFNTNCAIANGLLL 363

BLAST of CmaCh01G000450 vs. ExPASy TrEMBL
Match: A0A6J1GWE3 (protein BIG GRAIN 1-like B OS=Cucurbita moschata OX=3662 GN=LOC111457754 PE=3 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 2.5e-121
Identity = 253/379 (66.75%), Postives = 289/379 (76.25%), Query Frame = 0

Query: 1   MDLWDKSFQRHDQD-----LPQRRRSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFY 60
           MDLWD SFQR D D       QRRRSRR+NPSFSSSLLDAIYRSID+SN Q ED  +F+ 
Sbjct: 1   MDLWDNSFQREDPDPHHHHHHQRRRSRRENPSFSSSLLDAIYRSIDDSNAQGEDQLIFYT 60

Query: 61  RETTMRTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAG 120
           ++TT RTKQS+  A+TED + ++FRRA MIE WMD KK++ +RDFTVS+SSSSES+STAG
Sbjct: 61  QKTTTRTKQSISAAKTEDHQTLQFRRASMIENWMDNKKIKTLRDFTVSTSSSSESSSTAG 120

Query: 121 RRYSASESELLSRALHR------QKPIKTTPWTQTE-TQKAKHENGLVKTKSKASKIYHD 180
           RR S+SESE LSR LHR       KPIKTT W+QTE      HENG VK+K KASKIYHD
Sbjct: 121 RRLSSSESEFLSRTLHRPKPKPKPKPIKTTTWSQTEIIPNPNHENGFVKSKFKASKIYHD 180

Query: 181 LKKVKQPISPGGRLANFLNSLFNGGSPKTKQKMPNP-STNPTSMDYDMPTKAKSQ---GS 240
           LKKVKQPISPGGRLA+FLNSLFNGGSPK KQK+ N  S N T+++Y+ P K+KSQ    S
Sbjct: 181 LKKVKQPISPGGRLASFLNSLFNGGSPKAKQKISNTCSNNSTNLEYEAPRKSKSQQGSSS 240

Query: 241 TCSSASSFSRSCLSKTPSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGL 300
           TCSSASSFSRSCL KTPSSRGN+KRSVRFCPVS IVDEDC   GHK L KLEEPI KK  
Sbjct: 241 TCSSASSFSRSCLIKTPSSRGNIKRSVRFCPVSVIVDEDCRPCGHKMLNKLEEPIGKK-- 300

Query: 301 KSYGNFETEEVKTEHPRKIKRNEGFEYEDEDDAVSCSSSDLFELDNLSVIGIERFREELP 360
              G  ETEE               + +D+DD +SCSSSDLFELDNLS IG+ER+R+ELP
Sbjct: 301 ---GKLETEE--------------DDEDDDDDRLSCSSSDLFELDNLSAIGMERYRDELP 360

Query: 361 VYETTNFKTNCAIAKGLLV 364
           VYETT+F TNCAIA GLL+
Sbjct: 361 VYETTHFNTNCAIANGLLL 360

BLAST of CmaCh01G000450 vs. ExPASy TrEMBL
Match: A0A1S3CKK1 (protein BIG GRAIN 1-like B OS=Cucumis melo OX=3656 GN=LOC103501995 PE=3 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 1.0e-119
Identity = 264/385 (68.57%), Postives = 298/385 (77.40%), Query Frame = 0

Query: 1   MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRETTM 60
           MDLWD S       L Q RRSRRDNPSFSSSLLDAIYRSIDESN Q ED HL FY   T 
Sbjct: 1   MDLWDHS---DPHLLQQPRRSRRDNPSFSSSLLDAIYRSIDESNSQPED-HLIFYTHNT- 60

Query: 61  RTKQSVVTAR-TEDDEAVKFRRACMIEKWMD-RKKVRNIRDFTVSSSSSSESTSTAGRRY 120
            TK +++ A  ++D E++KFR   MI+ W+D +KK+RNIRDFT+SSSSSSES+STAGRR+
Sbjct: 61  -TKHTILPATVSQDHESLKFR---MIDTWIDKKKKLRNIRDFTLSSSSSSESSSTAGRRF 120

Query: 121 SASESELLSRALHR-------QKPIKTTPWTQTE--TQKAKHENGLVKTKSKASKIYHDL 180
           S+SE+E LSRALHR        KPIKT  W QTE  T   KH+NG VK+KSKASKIYHDL
Sbjct: 121 SSSETEFLSRALHRPTKLKPKPKPIKTNTWPQTEIITPNPKHDNGFVKSKSKASKIYHDL 180

Query: 181 KKVKQPISPGGRLANFLNSLFNGGSPKTKQKMPNP--STNPTSMDYDMPTKAKSQ---GS 240
           KKVKQPISPG RLA+FLNSLFNGGSPK KQK+ +   S N T  DYDM  K+KSQ    S
Sbjct: 181 KKVKQPISPGARLASFLNSLFNGGSPKAKQKISSSTCSINSTKFDYDMSRKSKSQQGSTS 240

Query: 241 TCSSASSFSRSCLSKTPSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKG- 300
           TCSSASSFSRSCLSKTPSSRGN+KRSVRFCPVS IVDEDC+  GHK L K EEPIMKKG 
Sbjct: 241 TCSSASSFSRSCLSKTPSSRGNIKRSVRFCPVSVIVDEDCQPCGHKILHKSEEPIMKKGN 300

Query: 301 -LKSYGNFETEEVKTEHPRKI----KRNEGFEYEDEDDAVSCSSSDLFELDNLSVIGIER 360
             KS GN E E+VKTE   KI    +  E  + +D+DDA+SCSSSDLFELDNLSVIGIER
Sbjct: 301 LKKSNGNLEREKVKTEDMMKINYEEEEEEDDDDDDDDDALSCSSSDLFELDNLSVIGIER 360

Query: 361 FREELPVYETTNFKTNCAIAKGLLV 364
           +REELPVYETT+FKTNCAIAKGL++
Sbjct: 361 YREELPVYETTHFKTNCAIAKGLVL 376

BLAST of CmaCh01G000450 vs. NCBI nr
Match: XP_022998766.1 (protein BIG GRAIN 1-like B [Cucurbita maxima])

HSP 1 Score: 706.1 bits (1821), Expect = 1.6e-199
Identity = 363/363 (100.00%), Postives = 363/363 (100.00%), Query Frame = 0

Query: 1   MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRETTM 60
           MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRETTM
Sbjct: 84  MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRETTM 143

Query: 61  RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA 120
           RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA
Sbjct: 144 RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA 203

Query: 121 SESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGG 180
           SESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGG
Sbjct: 204 SESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGG 263

Query: 181 RLANFLNSLFNGGSPKTKQKMPNPSTNPTSMDYDMPTKAKSQGSTCSSASSFSRSCLSKT 240
           RLANFLNSLFNGGSPKTKQKMPNPSTNPTSMDYDMPTKAKSQGSTCSSASSFSRSCLSKT
Sbjct: 264 RLANFLNSLFNGGSPKTKQKMPNPSTNPTSMDYDMPTKAKSQGSTCSSASSFSRSCLSKT 323

Query: 241 PSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGNFETEEVKTEHP 300
           PSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGNFETEEVKTEHP
Sbjct: 324 PSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGNFETEEVKTEHP 383

Query: 301 RKIKRNEGFEYEDEDDAVSCSSSDLFELDNLSVIGIERFREELPVYETTNFKTNCAIAKG 360
           RKIKRNEGFEYEDEDDAVSCSSSDLFELDNLSVIGIERFREELPVYETTNFKTNCAIAKG
Sbjct: 384 RKIKRNEGFEYEDEDDAVSCSSSDLFELDNLSVIGIERFREELPVYETTNFKTNCAIAKG 443

Query: 361 LLV 364
           LLV
Sbjct: 444 LLV 446

BLAST of CmaCh01G000450 vs. NCBI nr
Match: XP_022948938.1 (protein BIG GRAIN 1-like B [Cucurbita moschata])

HSP 1 Score: 682.9 bits (1761), Expect = 1.5e-192
Identity = 355/369 (96.21%), Postives = 358/369 (97.02%), Query Frame = 0

Query: 1   MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRETTM 60
           MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESN QDEDGHLFFYRETTM
Sbjct: 1   MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNDQDEDGHLFFYRETTM 60

Query: 61  RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA 120
           RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA
Sbjct: 61  RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA 120

Query: 121 SESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGG 180
           SESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGG
Sbjct: 121 SESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGG 180

Query: 181 RLANFLNSLFNGGSPKTKQKMPNPSTNPTSMDYDMPTKAKSQGSTCSSASSFSRSCLSKT 240
           RLANFLNSLFNGGSPKTKQKMPNPSTNP SMDYDMPTKAKSQGSTCSSASSFSRSCLSKT
Sbjct: 181 RLANFLNSLFNGGSPKTKQKMPNPSTNPRSMDYDMPTKAKSQGSTCSSASSFSRSCLSKT 240

Query: 241 PSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGNFETEEVKTEHP 300
           PSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYG FETEEVKTEHP
Sbjct: 241 PSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGKFETEEVKTEHP 300

Query: 301 RKIKRNEGFEYED------EDDAVSCSSSDLFELDNLSVIGIERFREELPVYETTNFKTN 360
           RK K NEGFEYED      EDDAVSCSSSDLFELDNLSVIG+ER+REELPVYETTNFKTN
Sbjct: 301 RKSKGNEGFEYEDEDEEEEEDDAVSCSSSDLFELDNLSVIGMERYREELPVYETTNFKTN 360

Query: 361 CAIAKGLLV 364
           CAIAKGL+V
Sbjct: 361 CAIAKGLVV 369

BLAST of CmaCh01G000450 vs. NCBI nr
Match: KAG6606698.1 (Protein BIG GRAIN 1-like A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 670.6 bits (1729), Expect = 7.5e-189
Identity = 349/369 (94.58%), Postives = 355/369 (96.21%), Query Frame = 0

Query: 1   MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRETTM 60
           MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESN QDEDGHLFFYRETTM
Sbjct: 1   MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNDQDEDGHLFFYRETTM 60

Query: 61  RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA 120
           RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA
Sbjct: 61  RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA 120

Query: 121 SESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGG 180
           SESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASK+YHDLKKVKQPISPGG
Sbjct: 121 SESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKVYHDLKKVKQPISPGG 180

Query: 181 RLANFLNSLFNGGSPKTKQKMPNPSTNPTSMDYDMPTKAKSQGSTCSSASSFSRSCLSKT 240
           RLANFLNSLFNGGSPKTKQK+PNPSTNP SMDYDM TKAKSQGSTCSSASSFSRSCLSKT
Sbjct: 181 RLANFLNSLFNGGSPKTKQKIPNPSTNPRSMDYDMATKAKSQGSTCSSASSFSRSCLSKT 240

Query: 241 PSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGNFETEEVKTEHP 300
           PSSRGNVKRSVRFCPVSEIV+EDCEAYG KSLQKLEEPIMKKGLKSYG FETEEVKTEHP
Sbjct: 241 PSSRGNVKRSVRFCPVSEIVEEDCEAYGQKSLQKLEEPIMKKGLKSYGKFETEEVKTEHP 300

Query: 301 RKIKRNEGFEYED------EDDAVSCSSSDLFELDNLSVIGIERFREELPVYETTNFKTN 360
           RK K NEGFEYED      EDDA SCSSSDLFELDNLSVIG+ER+REELPVYETTNFKTN
Sbjct: 301 RKSKGNEGFEYEDEDEEEEEDDAGSCSSSDLFELDNLSVIGMERYREELPVYETTNFKTN 360

Query: 361 CAIAKGLLV 364
           CAIAKGL+V
Sbjct: 361 CAIAKGLVV 369

BLAST of CmaCh01G000450 vs. NCBI nr
Match: XP_023525389.1 (protein BIG GRAIN 1-like B [Cucurbita pepo subsp. pepo])

HSP 1 Score: 667.9 bits (1722), Expect = 4.9e-188
Identity = 349/369 (94.58%), Postives = 353/369 (95.66%), Query Frame = 0

Query: 1   MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRETTM 60
           MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESN QDEDGHLFFYRETTM
Sbjct: 1   MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNDQDEDGHLFFYRETTM 60

Query: 61  RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA 120
           RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA
Sbjct: 61  RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA 120

Query: 121 SESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGG 180
           SESELLSRALHR KPIKTTPWTQTETQKAKHENGLVKTKSKASK+YHDLKKVKQPISPGG
Sbjct: 121 SESELLSRALHRPKPIKTTPWTQTETQKAKHENGLVKTKSKASKMYHDLKKVKQPISPGG 180

Query: 181 RLANFLNSLFNGGSPKTKQKMPNPSTNPTSMDYDMPTKAKSQGSTCSSASSFSRSCLSKT 240
           RLANFLNSLFNGGSPKTKQK PN STNP SMDYDMPTKAKSQGSTCSSASSFSRSCLSKT
Sbjct: 181 RLANFLNSLFNGGSPKTKQKTPNRSTNPRSMDYDMPTKAKSQGSTCSSASSFSRSCLSKT 240

Query: 241 PSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGNFETEEVKTEHP 300
           P SRGNVKRSVRFCPVSEIVDEDCEA GHKSLQKLEEPIMKKGLKSYG FETEEVKTEHP
Sbjct: 241 PCSRGNVKRSVRFCPVSEIVDEDCEADGHKSLQKLEEPIMKKGLKSYGKFETEEVKTEHP 300

Query: 301 RKIKRNEGFEYED------EDDAVSCSSSDLFELDNLSVIGIERFREELPVYETTNFKTN 360
           RK K NEGF+YED      EDDAVSCSSSDLFELDNLSVIG+ER+REELPVYETTNFKTN
Sbjct: 301 RKSKGNEGFDYEDEDEEEEEDDAVSCSSSDLFELDNLSVIGMERYREELPVYETTNFKTN 360

Query: 361 CAIAKGLLV 364
           CAIAKGLLV
Sbjct: 361 CAIAKGLLV 369

BLAST of CmaCh01G000450 vs. NCBI nr
Match: KAG7036418.1 (Protein BIG GRAIN 1-like A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 667.5 bits (1721), Expect = 6.4e-188
Identity = 349/370 (94.32%), Postives = 354/370 (95.68%), Query Frame = 0

Query: 1   MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRETTM 60
           MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESN QDEDGHLFFYRETTM
Sbjct: 1   MDLWDKSFQRHDQDLPQRRRSRRDNPSFSSSLLDAIYRSIDESNDQDEDGHLFFYRETTM 60

Query: 61  RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA 120
           RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA
Sbjct: 61  RTKQSVVTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSA 120

Query: 121 SESELLSRALHRQKPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGG 180
           SESELLSRALHRQK IKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGG
Sbjct: 121 SESELLSRALHRQKAIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGG 180

Query: 181 RLANFLNSLFNGGSPKTKQKMPNPSTNPTSMDYDMPTKAKSQGSTCSSASSFSRSCLSKT 240
           RLANFLNSLFNGGSPKTKQK+PNPSTNP SMDYDM TKAKSQGSTCSSASSFSRSCLSKT
Sbjct: 181 RLANFLNSLFNGGSPKTKQKIPNPSTNPRSMDYDMATKAKSQGSTCSSASSFSRSCLSKT 240

Query: 241 PSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKKGLKSYGNFETEEVKTEHP 300
           PSSRGNVKRSVRFCPVSEIV+EDCEAYG KSLQKLEEPIMKKGLKSYG FETEEVKTEHP
Sbjct: 241 PSSRGNVKRSVRFCPVSEIVEEDCEAYGQKSLQKLEEPIMKKGLKSYGKFETEEVKTEHP 300

Query: 301 RKIKRNEGFEYEDE-------DDAVSCSSSDLFELDNLSVIGIERFREELPVYETTNFKT 360
           RK K NEGFEYEDE       DDA SCSSSDLFELDNLSVIG+ER+REELPVYETTNFKT
Sbjct: 301 RKSKGNEGFEYEDEDEEEEEDDDAGSCSSSDLFELDNLSVIGMERYREELPVYETTNFKT 360

Query: 361 NCAIAKGLLV 364
           NCAIAKGL+V
Sbjct: 361 NCAIAKGLVV 370

BLAST of CmaCh01G000450 vs. TAIR 10
Match: AT1G54200.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 1084 Blast hits to 581 proteins in 136 species: Archae - 0; Bacteria - 72; Metazoa - 212; Fungi - 78; Plants - 102; Viruses - 0; Other Eukaryotes - 620 (source: NCBI BLink). )

HSP 1 Score: 208.8 bits (530), Expect = 7.5e-54
Identity = 176/406 (43.35%), Postives = 216/406 (53.20%), Query Frame = 0

Query: 1   MDLWDKSFQRHDQDLPQRRRSRRD--NPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRET 60
           MD WD        D   R R+RRD  +PSFSS+LLD IYRSID+S+           R+T
Sbjct: 1   MDPWDT--YNIPLDHQHRLRNRRDHRHPSFSSTLLDQIYRSIDDSSTNSSS-----MRKT 60

Query: 61  TMRTKQSV-VTARTEDDEAVKFRRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRR 120
             + ++   V+A   DD    F R+         K ++ I       SSSS S S+    
Sbjct: 61  KHQNREDTRVSANRRDD----FNRS---------KNLKTIEPVFFKHSSSSSSDSSG--- 120

Query: 121 YSASESELLSR------ALHRQKPIKTT-------PWTQTETQKAKHENG-LVKTKSKAS 180
           +S+SES+   R      A+   KPI+TT       P         K E+G  +KTKSKA 
Sbjct: 121 FSSSESDYFYRRSKSSPAISHPKPIRTTVERFERSPQNHRPNSSNKQEHGSFLKTKSKAL 180

Query: 181 KIYHDLKKVKQPISPGGRLANFLNSLFNG-GSPKTKQKMPNPSTNPTSMDYDMPTKAKSQ 240
           KIY DLKKVKQPISPGGRLA FLNS+F G G+ K   K+    T+ T+        A S 
Sbjct: 181 KIYSDLKKVKQPISPGGRLATFLNSIFTGAGNTKKLNKINTTVTSTTA-----AAAAASS 240

Query: 241 GSTCSSASSFSRSCLSKTPSSRGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPIMKK 300
            +TCSSASSFSRSCLSKTPSS    KRSVRFCPV+ I DED   Y +K+           
Sbjct: 241 TTTCSSASSFSRSCLSKTPSSSEKSKRSVRFCPVNVIFDEDSSKYNNKN----------- 300

Query: 301 GLKSYGNFETE----------EVKTEHPRKI-----------KRN--------EGFEYED 360
             K YGN E E           V  E+ R I           K+N        E  E +D
Sbjct: 301 -NKVYGNNEREYESIRHTLENRVMEENRRVIEAAKELLRSYQKKNKEVIEVSVEDDEEDD 360

BLAST of CmaCh01G000450 vs. TAIR 10
Match: AT3G13980.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G54200.1); Has 1485 Blast hits to 418 proteins in 98 species: Archae - 0; Bacteria - 6; Metazoa - 246; Fungi - 61; Plants - 107; Viruses - 6; Other Eukaryotes - 1059 (source: NCBI BLink). )

HSP 1 Score: 201.1 bits (510), Expect = 1.6e-51
Identity = 157/358 (43.85%), Postives = 199/358 (55.59%), Query Frame = 0

Query: 21  SRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRETTMRTKQSVVTARTEDDEAVKFR 80
           S   NPSFSS+LLD IYRSID+S+       +   +    +   S+   R  +   +  R
Sbjct: 12  SHHRNPSFSSTLLDQIYRSIDDSSPPPPLESIKKKKHHHQQRNASLHEDR--EISPIYHR 71

Query: 81  RACMIEKWMDRKKVRNIRDFTVSSS-----SSSESTSTAGRRYSASESELLSRALHRQKP 140
           R+   +    R+K   +R    SSS     SSSES S  GR  S++     SR   + KP
Sbjct: 72  RSIAADFERSRRKTDFLRHSNSSSSDSSGFSSSESDSFHGRSKSSASPPSSSR--QQPKP 131

Query: 141 IKTTPWTQTE-TQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGGRLANFLNSLFNGGS 200
           I+T+    +   QK K   G ++TKSKA KIY DLKKVKQPISPGGRLA FLNSLF   +
Sbjct: 132 IRTSSVDHSSAVQKPKELGGFLRTKSKALKIYSDLKKVKQPISPGGRLATFLNSLFTNAA 191

Query: 201 PKTKQKMPNPSTNPTSMDYDMPTKAKSQGSTCSSASSFSRSCLSKTPSSRGNVKRSVRFC 260
              K+   +  T   ++      +     STCSSASSFSRSCLSKTPSS G  KRSVRFC
Sbjct: 192 TNPKK---HKKTTTVAV-----VEEPHSSSTCSSASSFSRSCLSKTPSSSGKSKRSVRFC 251

Query: 261 PVSEIVDEDCE-----AYGHKSLQKLEEPIMKKGLKSYGNFETEEVKTEHPRK---IKRN 320
           PV+ I+DED       AY ++ L    E    +  +       + ++T H +        
Sbjct: 252 PVNVILDEDSSFTMPYAYNNERLYDNNEAKRVEEHRRVIQAAKDLLRTYHNKNKVTTTNI 311

Query: 321 EGFEYEDEDDAVSCSSSDLFELDNLSVIGIERFREELPVYETTNF-KTNCAIAKGLLV 364
              E +DEDDA SC+SSDLFEL+NLS IGIER+REELPVYETT     N  IA GL+V
Sbjct: 312 NNVEEDDEDDAASCASSDLFELENLSAIGIERYREELPVYETTRLDNMNRVIATGLIV 357

BLAST of CmaCh01G000450 vs. TAIR 10
Match: AT3G42800.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G54200.1); Has 853 Blast hits to 340 proteins in 98 species: Archae - 0; Bacteria - 94; Metazoa - 145; Fungi - 62; Plants - 95; Viruses - 0; Other Eukaryotes - 457 (source: NCBI BLink). )

HSP 1 Score: 145.6 bits (366), Expect = 7.8e-35
Identity = 136/375 (36.27%), Postives = 189/375 (50.40%), Query Frame = 0

Query: 16  PQRRRSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRETTMRTKQSVVTARTEDDE 75
           PQR+R+    PSFSSS+LD++YRSIDES+    D  L       + +  S  +   +DD+
Sbjct: 4   PQRKRT----PSFSSSVLDSVYRSIDESDGLQSD--LKGSINENVSSSSSSPSPNKKDDK 63

Query: 76  AVKFRRACMIEK-WM-DRKKVRNIRDFTVSSSSSSESTSTAGRRYSASESELLSRALHRQ 135
               RRA M E+ W+  R           SS SSSE+ S   +R     +E   R+   +
Sbjct: 64  LTTLRRAIMDEEHWLYARSSTTTTNSSDSSSFSSSEAESYRTKRRLRKLAEQGKRSGDER 123

Query: 136 KPIKTTPWTQTETQKAKHENGLVKTKSKASKIYHDLKKVKQPISPGGRLANFLNSLFNGG 195
           +  K T         +K ++     K KA KI  +LK+ KQP+SPG RL +FLNS+F   
Sbjct: 124 QRTKRTVMDNDSRLFSKSDD---DKKPKAVKIIEELKRSKQPVSPGARLTSFLNSIFQSN 183

Query: 196 SPKTKQKMPNPSTNPTSMDYDMPTKAKSQGSTCSSASSFSRSCLSKTPSSRGN------- 255
           + K                     K  S G T    SS S+SC S+T +   N       
Sbjct: 184 AKK--------------------VKLCSVGKTTDVKSSSSKSCFSRTRNKTDNNNNNCKK 243

Query: 256 VKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPI----MKKGLKSYGNFETEEVKT-EHPR 315
           ++RS+RF PV   +D DC  Y  K + ++ +PI     KK +K       EE+KT +H  
Sbjct: 244 LERSIRFYPVRVTIDGDCRDYAQKHITRVRKPIPEFTAKKSVK-------EEIKTNDHHT 303

Query: 316 K---IKRNEGFE----------YEDEDDAVSCSSSDLFELDNLSVIGIERFREELPVYET 364
           +   I RN G +           E+E+DA S SSSDLFELD+   IG+ R+ +ELPVYET
Sbjct: 304 EFTCITRNIGLKDFVRSNKYEGKEEEEDAWSHSSSDLFELDSYR-IGMGRYLKELPVYET 341

BLAST of CmaCh01G000450 vs. TAIR 10
Match: AT5G12050.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 114.0 bits (284), Expect = 2.5e-25
Identity = 133/389 (34.19%), Postives = 182/389 (46.79%), Query Frame = 0

Query: 20  RSRRDNPSFSSSLLDAIYRSIDESNHQDEDGHLFFYRETTMRTKQSVVTARTEDDEAVKF 79
           +S+R NPSFSS+LLD IY SID    + +    +     T   KQS+VT    D      
Sbjct: 3   KSKR-NPSFSSTLLDEIYNSIDPKTQKTQP---YVGSVNTTTKKQSIVTRSVPD------ 62

Query: 80  RRACMIEKWMDRKKVRNIRDFTVSSSSSSESTSTAGRRYSASESELL-------SRAL-- 139
                       +K+   R F  S SSSS+S S+    +S+S++EL        SR L  
Sbjct: 63  ------------RKIHRDR-FFGSVSSSSDSNSSI---FSSSDTELTHGKKTTSSRPLCF 122

Query: 140 --HRQKPIKTTPWTQTETQKA-----------------KHENGLVKTKSKAS-KIYHDLK 199
              + KP KT   T     +A                 +H+     T+++ S K   + K
Sbjct: 123 GPSKTKPRKTEDKTLFHQNRATRVYDDYDYASDVPKFNRHDENWENTRNRRSVKSSGNQK 182

Query: 200 KVKQPISPGGRLANFLNSLFNGGSPKTKQKMPNPS---------TNPTSMDYDMPTKAKS 259
           K K P SPGGR+ NFLNSLF+  S ++      P             TS DY   T    
Sbjct: 183 KPKTPASPGGRIVNFLNSLFSNNSKQSNAVKSYPRKTSYDDSAYVRKTSNDYHSST---- 242

Query: 260 QGSTCSSASSFSRSCLSK-TPSSRGNVKRSVRFCPVSEIV-------DEDCEAYGH--KS 319
             +TCSSASSFSRSC++K    S G +KRSVRF PV+ IV       +ED  + G+  KS
Sbjct: 243 --TTCSSASSFSRSCMNKGYEKSSGRIKRSVRFSPVNVIVPESFTSKEEDYFSNGNARKS 302

Query: 320 LQKLEEPIMKKGLKSYGNFETEEVKTEHPRKIKRNEGFE-YE------DEDDAVSCSSSD 350
           ++K  E   ++ ++        +    H   + +  GFE YE      D+DD  S SSSD
Sbjct: 303 VKKNVEDGGRRSVEEIAREFLRDYHKNHENSLVKTNGFEDYEDDDEDDDDDDVASDSSSD 356

BLAST of CmaCh01G000450 vs. TAIR 10
Match: AT1G69160.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 173 Blast hits to 172 proteins in 54 species: Archae - 0; Bacteria - 0; Metazoa - 25; Fungi - 33; Plants - 84; Viruses - 2; Other Eukaryotes - 29 (source: NCBI BLink). )

HSP 1 Score: 53.1 bits (126), Expect = 5.3e-07
Identity = 78/292 (26.71%), Postives = 124/292 (42.47%), Query Frame = 0

Query: 100 FTVSSSSSSESTSTAGRRYSASESE------LL--SRALHRQKPIKTTPWTQTETQKAKH 159
           F  + +SS +   T    Y+A+  E      +L   R +    PI+ +       Q    
Sbjct: 38  FGYNEASSGDHGHTQKYGYNAAREENPRRWGILGGGRRISLDLPIRCSEQVYHLQQDHHE 97

Query: 160 ENGLVKTKSKASKIYHDLKKVKQPISPGGRLANFLNSLFNGGSPKTKQKMPNPSTNPT-- 219
           ++ +   K +   + H     KQP SPGG++A+FLNSLF+    K  +      T PT  
Sbjct: 98  KHEVTTIKERLGNVRH-----KQPSSPGGKIASFLNSLFHQAGSKKNKSKSKSKTKPTDP 157

Query: 220 ------------------SMDYDMPTKAKSQGSTCSSASSFSRSCLSKTPSS-------- 279
                             S+ +   +   +  +T ++ASS S+S +S + S         
Sbjct: 158 EVEEEIPGGGWMRRRRRSSISHFFSSSRSTSTTTTTTASSSSKSLISSSSSGFRTPPPYL 217

Query: 280 ---RGNVKRSVRFCPVSEIVDEDCEAYGHKSLQKLEEPI-MKKGLKSYGNFETEEVKTEH 339
                N K+ + +   ++ V E+ E   +K    L+E + + + L       +++   + 
Sbjct: 218 NTPTKNYKQFLNYTSATKQVGEE-ETKTNKEYSWLDEKLKVMESLSENQRIWSDDEDIDD 277

Query: 340 PRKIKRNEGFEYEDEDDAV-SCSSSDLFELDNLSVIGIERFREELPVYETTN 351
            R+IKR      E EDD + S SSSDLFEL N      E  R  LPVYETTN
Sbjct: 278 DRRIKR------EGEDDGMESDSSSDLFELQN-----YELSRGGLPVYETTN 312

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SLL21.1e-5243.35Protein BIG GRAIN 1-like B OS=Arabidopsis thaliana OX=3702 GN=At1g54200 PE=1 SV=... [more]
Q9LVK22.2e-5043.85Protein BIG GRAIN 1-like A OS=Arabidopsis thaliana OX=3702 GN=At3g13980 PE=3 SV=... [more]
Q9M2B31.1e-3336.27Protein BIG GRAIN 1-like C OS=Arabidopsis thaliana OX=3702 GN=At3g42800 PE=2 SV=... [more]
Q9LYH03.6e-2434.19Protein BIG GRAIN 1-like D OS=Arabidopsis thaliana OX=3702 GN=At5g12050 PE=2 SV=... [more]
Q10R091.5e-2233.81Protein BIG GRAIN 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BG1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KB467.8e-200100.00protein BIG GRAIN 1-like B OS=Cucurbita maxima OX=3661 GN=LOC111493335 PE=3 SV=1[more]
A0A6J1GAP77.1e-19396.21protein BIG GRAIN 1-like B OS=Cucurbita moschata OX=3662 GN=LOC111452438 PE=3 SV... [more]
A0A6J1IZW59.1e-12467.55protein BIG GRAIN 1-like B OS=Cucurbita maxima OX=3661 GN=LOC111479960 PE=3 SV=1[more]
A0A6J1GWE32.5e-12166.75protein BIG GRAIN 1-like B OS=Cucurbita moschata OX=3662 GN=LOC111457754 PE=3 SV... [more]
A0A1S3CKK11.0e-11968.57protein BIG GRAIN 1-like B OS=Cucumis melo OX=3656 GN=LOC103501995 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022998766.11.6e-199100.00protein BIG GRAIN 1-like B [Cucurbita maxima][more]
XP_022948938.11.5e-19296.21protein BIG GRAIN 1-like B [Cucurbita moschata][more]
KAG6606698.17.5e-18994.58Protein BIG GRAIN 1-like A, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023525389.14.9e-18894.58protein BIG GRAIN 1-like B [Cucurbita pepo subsp. pepo][more]
KAG7036418.16.4e-18894.32Protein BIG GRAIN 1-like A, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT1G54200.17.5e-5443.35unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G13980.11.6e-5143.85unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G42800.17.8e-3536.27unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G12050.12.5e-2534.19unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G69160.15.3e-0726.71unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 101..120
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 139..159
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 216..247
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..27
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 101..123
NoneNo IPR availablePANTHERPTHR33541:SF2PROTEIN BIG GRAIN 1-LIKE Acoord: 14..362
IPR039621Protein BIG GRAIN 1-likePANTHERPTHR33541PROTEIN BIG GRAIN 1-LIKE A-RELATEDcoord: 14..362

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G000450.1CmaCh01G000450.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009734 auxin-activated signaling pathway
cellular_component GO:0005886 plasma membrane