CmaCh01G000150 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh01G000150
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionGDSL esterase/lipase 5-like
LocationCma_Chr01: 60426 .. 62208 (-)
RNA-Seq ExpressionCmaCh01G000150
SyntenyCmaCh01G000150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACATCACATCAGAACTCCCCAACTAATTGTGTTTGTCATTAATGGCGACTCAAAGCACTTGGCGTGGTCAACTAATTGAGTTGCTTCTGATCTTCTTCTACTTCTTCAACTTCATTTCCATATATTGCTTTGCTGAAGTTGAATTCAATGTTGTGCCTGAGAAGCATGCTGCTTTCTTCATCTTTGGGGACTCTTTCTTGGACGCTGGCAACAATAATTACATCAACACCACAACTCTTGACCAAGCTAACTTCTGGCCCTATGGCCAAACCCACTTCCGATTCCCCACTGGAAGATTTTCCGACGGTCGTTTGGTGTCTGACTTTATAGGTAACACTTCCCATATATTTGGTTTATGTCCTCTTCATCTCTGTTTTCTTGTCTTCTGCAGTCTCCACATATTTTCCTTCTCTTTTTCTCTCTATACATTTGGGCAAGGCTCAACTTTTCATGACTTTTGTTGCTCTATCTTTTAGCTGAATTCGCCAAGCTGCCTTTGATCCCACCCTTTCTTCAACCGGGTTTTCATCATTATCATAATGGAGCGAACTTTGCTTCGGCTGGAGCAGGTGCTTTATCCGAGACTTTTCATGGGTCGGTAAGAATAACGCATCCCCCCCACTCCTCACGACGCACCTTTTCCATATTAAATAGCAAACCAAATCATTATCAACTCTTCTTTCTGCACTATACACAAAAAGACCATCACTTTGATCTAAAAACTTTCAAACCTGGTTCATGATTAAGCAACTCGGTTGTGAACGTATTCAGGTGATCGAGCTGAAAACACAGATCAAGTACTTTAGGGAGGTAGTAGTGACATGGCTGAAACGAAAGTTGGGGAAAGCTGAAGGTGGGTTGATGCTGTCAAAGGCTGTGTATTTGTTCAGCGTCGGAACCAATGATTACATGAGCCTCTTCTTAACCGATTCTGCTTTTCTCAAATCCCACTCCAAATCCCAATATATTGAGTTGGTGATTGGGAACTTGAGTACTTCCATCAAAGTAAGCTACGCTTTTTTGCAGTCTTCAATTTGCATTCAAACACAGTAATGTGGATCCTTGTTTTCTTGGTCTTGACAGGAAGTATATGATAGCGGGGGCAGGAAATTCGGGTTCCTGAACTTGCCGCCTATTGGGTGCAGTCCAGGGATGAGAGATGAAGATGGCGGGTGTTTGGAAGAGCTGTCGGCGTATACAAGTGTTCACAACCAGAGATTGGTGAAGCTTCTTAAAGACTTGGAGAGGCAGCTGAAGGGGTTCAAATATTCACTCTACGACTTCAGTAGCAGCCTCACAGACAGAATGGAAAATCCATTCAAATATGGTAGCAGAACTGTCACTCCCTGTCTTATCATATCTTAAACTTTGATACAGAGATTTTGAAGTGTGTAGGTGTAATATAATATAATAATGTAGGGTTGAAGGAAGGGAAGGAGGCATGCTGTGGAACAGGGAGATTTAGAGGAGTTTTCAGCTGCGGAGGAAAAAGAGGAGTGAAGGAGTTTGAATTGTGTAAGAATCCGAATGAACATATCTTTTGGGATTCTTACCATCTCACTGAAAACCTCCACAAACAGCTTGCTCATGAAATTTGGCGTGGCTCATCCAGTCGCCATTCCTCTCTCAAGTATCTTTTTCAAACCATTTGAGAACACCATTGGCCATGAGTTGTAATCATAAATCATATCCATTTAATCCTTTCCTCTTTCTAATGGCTATTTTAAACTTGAAACAATGTAAGATCTGAAACAAATATACATTTAGAATTGAGAATTGGGT

mRNA sequence

AACATCACATCAGAACTCCCCAACTAATTGTGTTTGTCATTAATGGCGACTCAAAGCACTTGGCGTGGTCAACTAATTGAGTTGCTTCTGATCTTCTTCTACTTCTTCAACTTCATTTCCATATATTGCTTTGCTGAAGTTGAATTCAATGTTGTGCCTGAGAAGCATGCTGCTTTCTTCATCTTTGGGGACTCTTTCTTGGACGCTGGCAACAATAATTACATCAACACCACAACTCTTGACCAAGCTAACTTCTGGCCCTATGGCCAAACCCACTTCCGATTCCCCACTGGAAGATTTTCCGACGGTCGTTTGGTGTCTGACTTTATAGCTGAATTCGCCAAGCTGCCTTTGATCCCACCCTTTCTTCAACCGGGTTTTCATCATTATCATAATGGAGCGAACTTTGCTTCGGCTGGAGCAGGTGCTTTATCCGAGACTTTTCATGGGTCGGTGATCGAGCTGAAAACACAGATCAAGTACTTTAGGGAGGTAGTAGTGACATGGCTGAAACGAAAGTTGGGGAAAGCTGAAGGTGGGTTGATGCTGTCAAAGGCTGTGTATTTGTTCAGCGTCGGAACCAATGATTACATGAGCCTCTTCTTAACCGATTCTGCTTTTCTCAAATCCCACTCCAAATCCCAATATATTGAGTTGGTGATTGGGAACTTGAGTACTTCCATCAAAGAAGTATATGATAGCGGGGGCAGGAAATTCGGGTTCCTGAACTTGCCGCCTATTGGGTGCAGTCCAGGGATGAGAGATGAAGATGGCGGGTGTTTGGAAGAGCTGTCGGCGTATACAAGTGTTCACAACCAGAGATTGGTGAAGCTTCTTAAAGACTTGGAGAGGCAGCTGAAGGGGTTCAAATATTCACTCTACGACTTCAGTAGCAGCCTCACAGACAGAATGGAAAATCCATTCAAATATGGTAGCAGAACTGTCACTCCCTGTCTTATCATATCTTAAACTTTGATACAGAGATTTTGAAGTGTGTAGGTGTAATATAATATAATAATGTAGGGTTGAAGGAAGGGAAGGAGGCATGCTGTGGAACAGGGAGATTTAGAGGAGTTTTCAGCTGCGGAGGAAAAAGAGGAGTGAAGGAGTTTGAATTGTGTAAGAATCCGAATGAACATATCTTTTGGGATTCTTACCATCTCACTGAAAACCTCCACAAACAGCTTGCTCATGAAATTTGGCGTGGCTCATCCAGTCGCCATTCCTCTCTCAAGTATCTTTTTCAAACCATTTGAGAACACCATTGGCCATGAGTTGTAATCATAAATCATATCCATTTAATCCTTTCCTCTTTCTAATGGCTATTTTAAACTTGAAACAATGTAAGATCTGAAACAAATATACATTTAGAATTGAGAATTGGGT

Coding sequence (CDS)

ATGGCGACTCAAAGCACTTGGCGTGGTCAACTAATTGAGTTGCTTCTGATCTTCTTCTACTTCTTCAACTTCATTTCCATATATTGCTTTGCTGAAGTTGAATTCAATGTTGTGCCTGAGAAGCATGCTGCTTTCTTCATCTTTGGGGACTCTTTCTTGGACGCTGGCAACAATAATTACATCAACACCACAACTCTTGACCAAGCTAACTTCTGGCCCTATGGCCAAACCCACTTCCGATTCCCCACTGGAAGATTTTCCGACGGTCGTTTGGTGTCTGACTTTATAGCTGAATTCGCCAAGCTGCCTTTGATCCCACCCTTTCTTCAACCGGGTTTTCATCATTATCATAATGGAGCGAACTTTGCTTCGGCTGGAGCAGGTGCTTTATCCGAGACTTTTCATGGGTCGGTGATCGAGCTGAAAACACAGATCAAGTACTTTAGGGAGGTAGTAGTGACATGGCTGAAACGAAAGTTGGGGAAAGCTGAAGGTGGGTTGATGCTGTCAAAGGCTGTGTATTTGTTCAGCGTCGGAACCAATGATTACATGAGCCTCTTCTTAACCGATTCTGCTTTTCTCAAATCCCACTCCAAATCCCAATATATTGAGTTGGTGATTGGGAACTTGAGTACTTCCATCAAAGAAGTATATGATAGCGGGGGCAGGAAATTCGGGTTCCTGAACTTGCCGCCTATTGGGTGCAGTCCAGGGATGAGAGATGAAGATGGCGGGTGTTTGGAAGAGCTGTCGGCGTATACAAGTGTTCACAACCAGAGATTGGTGAAGCTTCTTAAAGACTTGGAGAGGCAGCTGAAGGGGTTCAAATATTCACTCTACGACTTCAGTAGCAGCCTCACAGACAGAATGGAAAATCCATTCAAATATGGTAGCAGAACTGTCACTCCCTGTCTTATCATATCTTAA

Protein sequence

MATQSTWRGQLIELLLIFFYFFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGNNNYINTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYHNGANFASAGAGALSETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLMLSKAVYLFSVGTNDYMSLFLTDSAFLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSPGMRDEDGGCLEELSAYTSVHNQRLVKLLKDLERQLKGFKYSLYDFSSSLTDRMENPFKYGSRTVTPCLIIS
Homology
BLAST of CmaCh01G000150 vs. ExPASy Swiss-Prot
Match: Q9SSA7 (GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2)

HSP 1 Score: 298.9 bits (764), Expect = 6.6e-80
Identity = 151/259 (58.30%), Postives = 190/259 (73.36%), Query Frame = 0

Query: 44  AFFIFGDSFLDAGNNNYINTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLP 103
           A F+FGDSFLDAGNNNYINTTTLDQANF PYGQT F  PTGRFSDGRL+SDFIAE+A LP
Sbjct: 48  ALFLFGDSFLDAGNNNYINTTTLDQANFPPYGQTFFGLPTGRFSDGRLISDFIAEYANLP 107

Query: 104 LIPPFLQPGFHHYH-NGANFASAGAGALSETFHGSVIELKTQIKYFREVVVTWLKRKLGK 163
           LIPPFL+PG       G NFASAGAGAL ETF GSVI L+TQ+ ++++V   W +   GK
Sbjct: 108 LIPPFLEPGNSQKKLYGVNFASAGAGALVETFQGSVINLRTQLDHYKKVERLW-RTNFGK 167

Query: 164 AEGGLMLSKAVYLFSVGTNDYMSLFLTDSAFLKSHSKSQYIELVIGNLSTSIKEVYDSGG 223
            E    +S+AVYL S+G+NDY S+FLT+ +     S SQ++++VIGNL+T I E+Y  GG
Sbjct: 168 EESKKRISRAVYLISIGSNDYSSIFLTNQSL--PISMSQHVDIVIGNLTTFIHEIYKIGG 227

Query: 224 RKFGFLNLPPIGCSPGMR----DEDGGCLEELSAYTSVHNQRLVKLLKDLERQLKGFKYS 283
           RKFGFLN+P +GC P +R      D  CL + S   S+HN+ L  LL  ++RQ+KGFK+S
Sbjct: 228 RKFGFLNVPDLGCFPALRILQPKNDDSCLRDASRLASMHNRALTNLLFQMQRQVKGFKFS 287

Query: 284 LYDFSSSLTDRMENPFKYG 298
           L+D + SL  RM++P K+G
Sbjct: 288 LFDMNKSLRLRMQHPSKFG 303

BLAST of CmaCh01G000150 vs. ExPASy Swiss-Prot
Match: Q9SYF0 (GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 2.4e-69
Identity = 141/292 (48.29%), Postives = 196/292 (67.12%), Query Frame = 0

Query: 15  LLIFFYFFNFI---SIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGNNNYINTTTLDQANF 74
           L+IFF +   I   SI C      N+V  + +A F+FGDS  DAGNNNYI+T    ++N+
Sbjct: 8   LIIFFAYTTIILIGSINCRDNNNNNLVTNQ-SALFVFGDSVFDAGNNNYIDTLPSFRSNY 67

Query: 75  WPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQP--GFHHYHNGANFASAGAGA 134
           WPYGQT F+FPTGR SDGR + DFIAE+A LPLIP +LQP  G + +  G +FASAGAGA
Sbjct: 68  WPYGQTTFKFPTGRVSDGRTIPDFIAEYAWLPLIPAYLQPSNGKNQFPYGVSFASAGAGA 127

Query: 135 LSETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLMLSKAVYLFSVGTNDYMSLFLT 194
           L  TF G VI LK+Q+  F++ V   L+  LG+A+G +++S+AVYLF +G NDY   F T
Sbjct: 128 LVGTFPGMVINLKSQLNNFKK-VEKLLRSTLGEAQGKMVISRAVYLFHIGVNDYQYPFST 187

Query: 195 DSAFLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSPGM----RDEDGG 254
           +S+  +S  +  Y++ V+GN +  IKEVY  GGRKFGFLN+    C+P      + + G 
Sbjct: 188 NSSIFQSSPQEIYVDFVVGNTTAVIKEVYKIGGRKFGFLNMGAYDCAPASLIIDQTKIGT 247

Query: 255 CLEELSAYTSVHNQRLVKLLKDLERQLKGFKYSLYDFSSSLTDRMENPFKYG 298
           C + ++   ++HN++L   L+ LER+L GFKY+L+D+ +SL+ RM NP KYG
Sbjct: 248 CFKPVTELINLHNEKLESGLRRLERELSGFKYALHDYHTSLSVRMNNPSKYG 297

BLAST of CmaCh01G000150 vs. ExPASy Swiss-Prot
Match: Q9FLN0 (GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 2.6e-68
Identity = 135/288 (46.88%), Postives = 190/288 (65.97%), Query Frame = 0

Query: 16  LIFFYFFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGNNNYINTTTLDQANFWPYG 75
           + F  +   ISI     ++ N +    +A F+FGDS  DAGNNNYI+T +  ++N+WPYG
Sbjct: 9   ITFLAYTIIISIGSINCIDNNNLVTNQSALFVFGDSVFDAGNNNYIDTLSSVRSNYWPYG 68

Query: 76  QTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQP--GFHHYHNGANFASAGAGALSET 135
           QT F+ PTGR SDGRL+ DFIAE+A LPLIPP LQP  G   +  G NFAS GAGAL  T
Sbjct: 69  QTTFKSPTGRVSDGRLIPDFIAEYAWLPLIPPNLQPFNGNSQFAYGVNFASGGAGALVGT 128

Query: 136 FHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLMLSKAVYLFSVGTNDYMSLFLTDSAF 195
           F G VI L+TQ+  F++ V   L+ KLG AEG  ++S+AVYLF +G NDY   F T+S+ 
Sbjct: 129 FSGLVINLRTQLNNFKK-VEEMLRSKLGDAEGKRVISRAVYLFHIGLNDYQYPFTTNSSL 188

Query: 196 LKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSPGM----RDEDGGCLEE 255
            +S S  +Y++ V+GN++   KEVY+ GGRKFG LN  P  C+P      + +   C + 
Sbjct: 189 FQSISNEKYVDYVVGNMTDVFKEVYNLGGRKFGILNTGPYDCAPASLVIDQTKIRSCFQP 248

Query: 256 LSAYTSVHNQRLVKLLKDLERQLKGFKYSLYDFSSSLTDRMENPFKYG 298
           ++   ++HN++L+  L+ L  +L GFKY+L+D+ +SL++RM +P KYG
Sbjct: 249 VTELINMHNEKLLNGLRRLNHELSGFKYALHDYHTSLSERMNDPSKYG 295

BLAST of CmaCh01G000150 vs. ExPASy Swiss-Prot
Match: H6U1I8 (GDSL lipase OS=Tanacetum cinerariifolium OX=118510 GN=GLIP PE=1 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 5.8e-68
Identity = 130/275 (47.27%), Postives = 188/275 (68.36%), Query Frame = 0

Query: 25  ISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGNNNYINTTTLDQANFWPYGQTHFRFPTG 84
           +++ C +     +  ++ AA FIFGDS  D GNNN+INT    +ANFWPYGQ++F  PTG
Sbjct: 14  VAVLCLSLPTGCLSSQQAAALFIFGDSVFDPGNNNHINTHVNFKANFWPYGQSYFSSPTG 73

Query: 85  RFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYHNGANFASAGAGALSETFHGSVIELKTQ 144
           RFSDGR++ DFIAE+A LP+IP +L+P  + + +GANFASAGAGAL  +  G  + L+TQ
Sbjct: 74  RFSDGRIIPDFIAEYASLPIIPAYLEPN-NDFTHGANFASAGAGALIASHAGLAVGLQTQ 133

Query: 145 IKYFREVVVTWLKRKLGKAEGGLMLSKAVYLFSVGTNDYMSLFLTDSAFLKSHSKSQYIE 204
           ++YF ++V  + ++ LG  +   +LS AVYLFS G NDY S +         +++ QY++
Sbjct: 134 LRYFGDLVDHY-RQNLGDIKSRQLLSDAVYLFSCGGNDYQSPYY-------PYTQEQYVD 193

Query: 205 LVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSPGMRDEDGG--CLEELSAYTSVHNQRLV 264
           +VIGN++  IK +Y+ GGRKFG +N+P IGC PGMR +  G  C  E+   T +HNQ   
Sbjct: 194 IVIGNMTNVIKGIYEKGGRKFGVVNVPLIGCWPGMRAKQPGNTCNTEVDELTRLHNQAFA 253

Query: 265 KLLKDLERQLKGFKYSLYDFSSSLTDRMENPFKYG 298
           K L+ LE+QL+GF Y+ +D S+++ +RM+NP KYG
Sbjct: 254 KRLEQLEKQLEGFVYAKFDLSTAILNRMKNPSKYG 279

BLAST of CmaCh01G000150 vs. ExPASy Swiss-Prot
Match: Q9SYF5 (GDSL esterase/lipase 3 OS=Arabidopsis thaliana OX=3702 GN=GLIP3 PE=2 SV=2)

HSP 1 Score: 252.7 bits (644), Expect = 5.4e-66
Identity = 135/290 (46.55%), Postives = 192/290 (66.21%), Query Frame = 0

Query: 14  LLLIFFYFFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGNNNYINTTTLDQANFWP 73
           +L+IFF +   +SI     ++ N +    AA F+FGDS  DAGNNNYINT +  ++N WP
Sbjct: 5   VLIIFFVYTIILSIGSINCIDNNNLVTNQAALFVFGDSLFDAGNNNYINTVSSFRSNIWP 64

Query: 74  YGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQP--GFHHYHNGANFASAGAGALS 133
           YGQT+F+FPTGR SDG        E A LP IPP LQP  G + +  G +FASAGAGAL+
Sbjct: 65  YGQTNFKFPTGRLSDG-------PEKAWLPSIPPNLQPNNGNNQFTYGVSFASAGAGALA 124

Query: 134 ETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLMLSKAVYLFSVGTNDYMSLFLTDS 193
           E+F G VI L TQ+  F++V  + L+ +LG AE   + S+AVYLF +G NDY   F  +S
Sbjct: 125 ESFLGMVINLGTQLNNFKDVEKS-LRSELGDAETKRVFSRAVYLFHIGANDYFYPFSANS 184

Query: 194 AFLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSPGM----RDEDGGCL 253
           +  KS+SK ++++ VIGN++  I+EVY  GGRKFGFLN+ P  CSP      R + G C 
Sbjct: 185 STFKSNSKEKFVDFVIGNITFVIEEVYKMGGRKFGFLNVGPYECSPNSLIRDRTKIGSCF 244

Query: 254 EELSAYTSVHNQRLVKLLKDLERQLKGFKYSLYDFSSSLTDRMENPFKYG 298
           + ++    +HN++   +L+ L+RQL GF+Y+L+D+ +SL++R+ +P KYG
Sbjct: 245 KPVAELIDMHNKKFPDVLRRLQRQLSGFRYALHDYHTSLSERINSPSKYG 286

BLAST of CmaCh01G000150 vs. ExPASy TrEMBL
Match: A0A6J1KBX9 (GDSL esterase/lipase 5-like OS=Cucurbita maxima OX=3661 GN=LOC111492922 PE=3 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 1.9e-170
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 0

Query: 1   MATQSTWRGQLIELLLIFFYFFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGNNNY 60
           MATQSTWRGQLIELLLIFFYFFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGNNNY
Sbjct: 1   MATQSTWRGQLIELLLIFFYFFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGNNNY 60

Query: 61  INTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYHNGA 120
           INTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYHNGA
Sbjct: 61  INTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYHNGA 120

Query: 121 NFASAGAGALSETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLMLSKAVYLFSVGT 180
           NFASAGAGALSETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLMLSKAVYLFSVGT
Sbjct: 121 NFASAGAGALSETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLMLSKAVYLFSVGT 180

Query: 181 NDYMSLFLTDSAFLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSPGMR 240
           NDYMSLFLTDSAFLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSPGMR
Sbjct: 181 NDYMSLFLTDSAFLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSPGMR 240

Query: 241 DEDGGCLEELSAYTSVHNQRLVKLLKDLERQLKGFKYSLYDFSSSLTDRMENPFKYG 298
           DEDGGCLEELSAYTSVHNQRLVKLLKDLERQLKGFKYSLYDFSSSLTDRMENPFKYG
Sbjct: 241 DEDGGCLEELSAYTSVHNQRLVKLLKDLERQLKGFKYSLYDFSSSLTDRMENPFKYG 297

BLAST of CmaCh01G000150 vs. ExPASy TrEMBL
Match: A0A6J1GAV1 (GDSL esterase/lipase 5-like OS=Cucurbita moschata OX=3662 GN=LOC111452496 PE=3 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 1.5e-164
Identity = 288/300 (96.00%), Postives = 294/300 (98.00%), Query Frame = 0

Query: 1   MATQSTWRGQLIELLLI---FFYFFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGN 60
           MATQSTWRGQLIELLLI   FF+FFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGN
Sbjct: 1   MATQSTWRGQLIELLLIFFFFFFFFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGN 60

Query: 61  NNYINTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYH 120
           NNYINTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYH
Sbjct: 61  NNYINTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYH 120

Query: 121 NGANFASAGAGALSETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLMLSKAVYLFS 180
           NGANFASAGAGALSETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLM+SKAVYLFS
Sbjct: 121 NGANFASAGAGALSETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLMVSKAVYLFS 180

Query: 181 VGTNDYMSLFLTDSAFLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSP 240
           +GTNDYMSLFLT+S FLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSP
Sbjct: 181 IGTNDYMSLFLTNSTFLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSP 240

Query: 241 GMRDEDGGCLEELSAYTSVHNQRLVKLLKDLERQLKGFKYSLYDFSSSLTDRMENPFKYG 298
           GMRDEDGGCLEELSAY SVHNQRLVKLL+DLE+QLKGFKYSLYDFSSSLT RMENPFKYG
Sbjct: 241 GMRDEDGGCLEELSAYKSVHNQRLVKLLQDLEKQLKGFKYSLYDFSSSLTHRMENPFKYG 300

BLAST of CmaCh01G000150 vs. ExPASy TrEMBL
Match: A0A5D3D6J2 (GDSL esterase/lipase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00670 PE=3 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 3.0e-136
Identity = 244/297 (82.15%), Postives = 262/297 (88.22%), Query Frame = 0

Query: 1   MATQSTWRGQLIELLLIFFYFFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGNNNY 60
           MATQST  GQL+ L   FF    F SI  F EVE   +PE HAAFFIFGDSFLDAGNNNY
Sbjct: 1   MATQSTSHGQLVVLCATFFI---FSSIRWFIEVESYSLPENHAAFFIFGDSFLDAGNNNY 60

Query: 61  INTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYHNGA 120
           INTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLI PFLQPGFH YH G 
Sbjct: 61  INTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLISPFLQPGFHQYHYGV 120

Query: 121 NFASAGAGALSETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLMLSKAVYLFSVGT 180
           NFASAGAGALSETFHGSVIELK QIKYF+E V TWLKRKLGKAEGGL+LSKAVYLF +GT
Sbjct: 121 NFASAGAGALSETFHGSVIELKAQIKYFKEDVETWLKRKLGKAEGGLVLSKAVYLFGIGT 180

Query: 181 NDYMSLFLTDSAFLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSPGMR 240
           NDYMSLFLT+S FLKSHSKSQY++LVIGNL+TSIK+VYDSGGRKFGF+NLPP+GCSPG+R
Sbjct: 181 NDYMSLFLTNSPFLKSHSKSQYVDLVIGNLTTSIKQVYDSGGRKFGFMNLPPMGCSPGLR 240

Query: 241 DEDGGCLEELSAYTSVHNQRLVKLLKDLERQLKGFKYSLYDFSSSLTDRMENPFKYG 298
            E G CLEEL+ Y +VHNQRLVK+L+DLE+QLKGFKYSLYDFSSSL  RMENP KYG
Sbjct: 241 GERGECLEELAEYANVHNQRLVKVLEDLEKQLKGFKYSLYDFSSSLRQRMENPLKYG 294

BLAST of CmaCh01G000150 vs. ExPASy TrEMBL
Match: A0A1S3CCC4 (GDSL esterase/lipase 5 OS=Cucumis melo OX=3656 GN=LOC103499192 PE=3 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 3.0e-136
Identity = 244/297 (82.15%), Postives = 262/297 (88.22%), Query Frame = 0

Query: 1   MATQSTWRGQLIELLLIFFYFFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGNNNY 60
           MATQST  GQL+ L   FF    F SI  F EVE   +PE HAAFFIFGDSFLDAGNNNY
Sbjct: 1   MATQSTSHGQLVVLCATFFI---FSSIRWFIEVESYSLPENHAAFFIFGDSFLDAGNNNY 60

Query: 61  INTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYHNGA 120
           INTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLI PFLQPGFH YH G 
Sbjct: 61  INTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLISPFLQPGFHQYHYGV 120

Query: 121 NFASAGAGALSETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLMLSKAVYLFSVGT 180
           NFASAGAGALSETFHGSVIELK QIKYF+E V TWLKRKLGKAEGGL+LSKAVYLF +GT
Sbjct: 121 NFASAGAGALSETFHGSVIELKAQIKYFKEDVETWLKRKLGKAEGGLVLSKAVYLFGIGT 180

Query: 181 NDYMSLFLTDSAFLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSPGMR 240
           NDYMSLFLT+S FLKSHSKSQY++LVIGNL+TSIK+VYDSGGRKFGF+NLPP+GCSPG+R
Sbjct: 181 NDYMSLFLTNSPFLKSHSKSQYVDLVIGNLTTSIKQVYDSGGRKFGFMNLPPMGCSPGLR 240

Query: 241 DEDGGCLEELSAYTSVHNQRLVKLLKDLERQLKGFKYSLYDFSSSLTDRMENPFKYG 298
            E G CLEEL+ Y +VHNQRLVK+L+DLE+QLKGFKYSLYDFSSSL  RMENP KYG
Sbjct: 241 GERGECLEELAEYANVHNQRLVKVLEDLEKQLKGFKYSLYDFSSSLRQRMENPLKYG 294

BLAST of CmaCh01G000150 vs. ExPASy TrEMBL
Match: A0A6J1GY70 (GDSL esterase/lipase 5-like OS=Cucurbita moschata OX=3662 GN=LOC111457918 PE=3 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 2.0e-135
Identity = 245/301 (81.40%), Postives = 263/301 (87.38%), Query Frame = 0

Query: 1   MATQSTWRGQLIELLLIFFYFFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGNNNY 60
           M TQST   QL+ L LIF  FF F S  CF EVE + +PE HAAFFIFGDSFLDAGNNNY
Sbjct: 1   METQSTSHEQLVVLFLIF--FFTFSSTRCFIEVESHPLPENHAAFFIFGDSFLDAGNNNY 60

Query: 61  INTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYHNGA 120
           INTTTLDQANFWPYGQTHFR+PTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFH YHNG 
Sbjct: 61  INTTTLDQANFWPYGQTHFRYPTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHQYHNGV 120

Query: 121 NFASAGAGALSETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLMLSKAVYLFSVGT 180
           NFASAGAGALSETFHGSVIELK QIKYF+E V TWLKRKL KAEGGL+LSKAVYLFS+GT
Sbjct: 121 NFASAGAGALSETFHGSVIELKAQIKYFKE-VETWLKRKLWKAEGGLILSKAVYLFSIGT 180

Query: 181 NDYMSLFLTDSAFLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSPGMR 240
           NDYMSLFLT S FLKSHS S+Y+ELVIGNL+TSIK+VYDSGGRKFGF+NLPP+GCSPG+R
Sbjct: 181 NDYMSLFLTKSPFLKSHSISEYVELVIGNLTTSIKQVYDSGGRKFGFMNLPPMGCSPGLR 240

Query: 241 ----DEDGGCLEELSAYTSVHNQRLVKLLKDLERQLKGFKYSLYDFSSSLTDRMENPFKY 298
               D DG CLEE  AY + HNQRLVKLL+DLE+QLKGFKYSLYDF+SSL  RMENP KY
Sbjct: 241 VAGGDADGRCLEEFGAYANEHNQRLVKLLQDLEKQLKGFKYSLYDFTSSLRQRMENPLKY 298

BLAST of CmaCh01G000150 vs. NCBI nr
Match: XP_022998205.1 (GDSL esterase/lipase 5-like [Cucurbita maxima])

HSP 1 Score: 608.2 bits (1567), Expect = 3.9e-170
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 0

Query: 1   MATQSTWRGQLIELLLIFFYFFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGNNNY 60
           MATQSTWRGQLIELLLIFFYFFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGNNNY
Sbjct: 1   MATQSTWRGQLIELLLIFFYFFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGNNNY 60

Query: 61  INTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYHNGA 120
           INTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYHNGA
Sbjct: 61  INTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYHNGA 120

Query: 121 NFASAGAGALSETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLMLSKAVYLFSVGT 180
           NFASAGAGALSETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLMLSKAVYLFSVGT
Sbjct: 121 NFASAGAGALSETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLMLSKAVYLFSVGT 180

Query: 181 NDYMSLFLTDSAFLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSPGMR 240
           NDYMSLFLTDSAFLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSPGMR
Sbjct: 181 NDYMSLFLTDSAFLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSPGMR 240

Query: 241 DEDGGCLEELSAYTSVHNQRLVKLLKDLERQLKGFKYSLYDFSSSLTDRMENPFKYG 298
           DEDGGCLEELSAYTSVHNQRLVKLLKDLERQLKGFKYSLYDFSSSLTDRMENPFKYG
Sbjct: 241 DEDGGCLEELSAYTSVHNQRLVKLLKDLERQLKGFKYSLYDFSSSLTDRMENPFKYG 297

BLAST of CmaCh01G000150 vs. NCBI nr
Match: XP_022949031.1 (GDSL esterase/lipase 5-like [Cucurbita moschata])

HSP 1 Score: 588.6 bits (1516), Expect = 3.2e-164
Identity = 288/300 (96.00%), Postives = 294/300 (98.00%), Query Frame = 0

Query: 1   MATQSTWRGQLIELLLI---FFYFFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGN 60
           MATQSTWRGQLIELLLI   FF+FFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGN
Sbjct: 1   MATQSTWRGQLIELLLIFFFFFFFFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGN 60

Query: 61  NNYINTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYH 120
           NNYINTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYH
Sbjct: 61  NNYINTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYH 120

Query: 121 NGANFASAGAGALSETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLMLSKAVYLFS 180
           NGANFASAGAGALSETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLM+SKAVYLFS
Sbjct: 121 NGANFASAGAGALSETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLMVSKAVYLFS 180

Query: 181 VGTNDYMSLFLTDSAFLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSP 240
           +GTNDYMSLFLT+S FLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSP
Sbjct: 181 IGTNDYMSLFLTNSTFLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSP 240

Query: 241 GMRDEDGGCLEELSAYTSVHNQRLVKLLKDLERQLKGFKYSLYDFSSSLTDRMENPFKYG 298
           GMRDEDGGCLEELSAY SVHNQRLVKLL+DLE+QLKGFKYSLYDFSSSLT RMENPFKYG
Sbjct: 241 GMRDEDGGCLEELSAYKSVHNQRLVKLLQDLEKQLKGFKYSLYDFSSSLTHRMENPFKYG 300

BLAST of CmaCh01G000150 vs. NCBI nr
Match: KAG6606674.1 (GDSL esterase/lipase 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 586.6 bits (1511), Expect = 1.2e-163
Identity = 289/301 (96.01%), Postives = 296/301 (98.34%), Query Frame = 0

Query: 1   MATQSTWRGQLIELLLI---FFYFFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGN 60
           MATQSTWRGQLIELLLI   FF+FFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGN
Sbjct: 1   MATQSTWRGQLIELLLIFFFFFFFFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGN 60

Query: 61  NNYINTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYH 120
           NNYINTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYH
Sbjct: 61  NNYINTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYH 120

Query: 121 NGANFASAGAGALSETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLMLSKAVYLFS 180
           NGANFASAGAGALSETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLM+SKAVYLFS
Sbjct: 121 NGANFASAGAGALSETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLMVSKAVYLFS 180

Query: 181 VGTNDYMSLFLTDSA-FLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCS 240
           +GTNDYMSLFLT+S+ FLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCS
Sbjct: 181 IGTNDYMSLFLTNSSTFLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCS 240

Query: 241 PGMRDEDGGCLEELSAYTSVHNQRLVKLLKDLERQLKGFKYSLYDFSSSLTDRMENPFKY 298
           PGMRDEDGGCLEELSAYTSVHNQRLVKLL+DLE+QLKGFKYSLYDFSSSLT RMENPFKY
Sbjct: 241 PGMRDEDGGCLEELSAYTSVHNQRLVKLLQDLEKQLKGFKYSLYDFSSSLTHRMENPFKY 300

BLAST of CmaCh01G000150 vs. NCBI nr
Match: XP_023525118.1 (GDSL esterase/lipase 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 582.8 bits (1501), Expect = 1.7e-162
Identity = 285/300 (95.00%), Postives = 291/300 (97.00%), Query Frame = 0

Query: 1   MATQSTWRGQLIELLLI---FFYFFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGN 60
           MATQSTWRGQLIELLLI   FF+FFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGN
Sbjct: 1   MATQSTWRGQLIELLLIFFFFFFFFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGN 60

Query: 61  NNYINTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYH 120
           NNYINTTTLDQANFWPYGQTHFR PTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYH
Sbjct: 61  NNYINTTTLDQANFWPYGQTHFRLPTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYH 120

Query: 121 NGANFASAGAGALSETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLMLSKAVYLFS 180
           NGANFASAGAGALSETFHGSVIELK QIKYFREVVVTWLKRK GKAEGGLM+SKAVYLFS
Sbjct: 121 NGANFASAGAGALSETFHGSVIELKAQIKYFREVVVTWLKRKSGKAEGGLMVSKAVYLFS 180

Query: 181 VGTNDYMSLFLTDSAFLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSP 240
           +GTNDYMSLFLT+S FLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSP
Sbjct: 181 IGTNDYMSLFLTNSTFLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSP 240

Query: 241 GMRDEDGGCLEELSAYTSVHNQRLVKLLKDLERQLKGFKYSLYDFSSSLTDRMENPFKYG 298
           GMRDEDGGCLEELSAYTSVHNQRL KLL+DLE+QLKGFKYSLYDFSSSLT RMENPFKYG
Sbjct: 241 GMRDEDGGCLEELSAYTSVHNQRLAKLLQDLEKQLKGFKYSLYDFSSSLTHRMENPFKYG 300

BLAST of CmaCh01G000150 vs. NCBI nr
Match: XP_038904622.1 (GDSL esterase/lipase 5 [Benincasa hispida])

HSP 1 Score: 499.6 bits (1285), Expect = 1.9e-137
Identity = 244/303 (80.53%), Postives = 268/303 (88.45%), Query Frame = 0

Query: 1   MATQSTWRGQLIELLLIFFYFFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGNNNY 60
           MATQST  G   +L+++F  FF + SI CF EVE + +PE HAAFFIFGDSFLDAGNNNY
Sbjct: 1   MATQSTCHGH-AQLVVLFVIFFTYSSICCFIEVESHHLPENHAAFFIFGDSFLDAGNNNY 60

Query: 61  INTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYHNGA 120
           INTTTLDQANFWPYG+THF FPTGRFSDGRLVSDF+AEFAKLPLIPPFLQPGF  YHNG 
Sbjct: 61  INTTTLDQANFWPYGETHFLFPTGRFSDGRLVSDFLAEFAKLPLIPPFLQPGFCQYHNGV 120

Query: 121 NFASAGAGALSETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLMLSKAVYLFSVGT 180
           NFASAGAGALSETFHGSVIELKTQIKYF+EVV TW  RKLGKAEGGL+LSKAVYLF +GT
Sbjct: 121 NFASAGAGALSETFHGSVIELKTQIKYFKEVVETWFTRKLGKAEGGLVLSKAVYLFGIGT 180

Query: 181 NDYMSLFLTDSAFLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSPGMR 240
           NDYMSLFLT+S FLKSHSKSQY+ELVIGNL+TSI+ VYDSGGRKFGF+NLPP+GCSPG+R
Sbjct: 181 NDYMSLFLTNSPFLKSHSKSQYVELVIGNLTTSIEHVYDSGGRKFGFMNLPPMGCSPGLR 240

Query: 241 ----DEDGGCLEELSAYTSVHNQRLVKLLKDLERQLKGFKYSLYDFSSSLTDRMENPFKY 300
               DEDG CLEEL+ Y ++HNQRLVKLL+DLE+QLKGFKYSLYDFSSSL  RMENP KY
Sbjct: 241 VVRGDEDGRCLEELAEYANIHNQRLVKLLEDLEKQLKGFKYSLYDFSSSLRQRMENPLKY 300

BLAST of CmaCh01G000150 vs. TAIR 10
Match: AT1G53920.1 (GDSL-motif lipase 5 )

HSP 1 Score: 298.9 bits (764), Expect = 4.7e-81
Identity = 151/259 (58.30%), Postives = 190/259 (73.36%), Query Frame = 0

Query: 44  AFFIFGDSFLDAGNNNYINTTTLDQANFWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLP 103
           A F+FGDSFLDAGNNNYINTTTLDQANF PYGQT F  PTGRFSDGRL+SDFIAE+A LP
Sbjct: 48  ALFLFGDSFLDAGNNNYINTTTLDQANFPPYGQTFFGLPTGRFSDGRLISDFIAEYANLP 107

Query: 104 LIPPFLQPGFHHYH-NGANFASAGAGALSETFHGSVIELKTQIKYFREVVVTWLKRKLGK 163
           LIPPFL+PG       G NFASAGAGAL ETF GSVI L+TQ+ ++++V   W +   GK
Sbjct: 108 LIPPFLEPGNSQKKLYGVNFASAGAGALVETFQGSVINLRTQLDHYKKVERLW-RTNFGK 167

Query: 164 AEGGLMLSKAVYLFSVGTNDYMSLFLTDSAFLKSHSKSQYIELVIGNLSTSIKEVYDSGG 223
            E    +S+AVYL S+G+NDY S+FLT+ +     S SQ++++VIGNL+T I E+Y  GG
Sbjct: 168 EESKKRISRAVYLISIGSNDYSSIFLTNQSL--PISMSQHVDIVIGNLTTFIHEIYKIGG 227

Query: 224 RKFGFLNLPPIGCSPGMR----DEDGGCLEELSAYTSVHNQRLVKLLKDLERQLKGFKYS 283
           RKFGFLN+P +GC P +R      D  CL + S   S+HN+ L  LL  ++RQ+KGFK+S
Sbjct: 228 RKFGFLNVPDLGCFPALRILQPKNDDSCLRDASRLASMHNRALTNLLFQMQRQVKGFKFS 287

Query: 284 LYDFSSSLTDRMENPFKYG 298
           L+D + SL  RM++P K+G
Sbjct: 288 LFDMNKSLRLRMQHPSKFG 303

BLAST of CmaCh01G000150 vs. TAIR 10
Match: AT1G53940.1 (GDSL-motif lipase 2 )

HSP 1 Score: 263.8 bits (673), Expect = 1.7e-70
Identity = 141/292 (48.29%), Postives = 196/292 (67.12%), Query Frame = 0

Query: 15  LLIFFYFFNFI---SIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGNNNYINTTTLDQANF 74
           L+IFF +   I   SI C      N+V  + +A F+FGDS  DAGNNNYI+T    ++N+
Sbjct: 8   LIIFFAYTTIILIGSINCRDNNNNNLVTNQ-SALFVFGDSVFDAGNNNYIDTLPSFRSNY 67

Query: 75  WPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQP--GFHHYHNGANFASAGAGA 134
           WPYGQT F+FPTGR SDGR + DFIAE+A LPLIP +LQP  G + +  G +FASAGAGA
Sbjct: 68  WPYGQTTFKFPTGRVSDGRTIPDFIAEYAWLPLIPAYLQPSNGKNQFPYGVSFASAGAGA 127

Query: 135 LSETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLMLSKAVYLFSVGTNDYMSLFLT 194
           L  TF G VI LK+Q+  F++ V   L+  LG+A+G +++S+AVYLF +G NDY   F T
Sbjct: 128 LVGTFPGMVINLKSQLNNFKK-VEKLLRSTLGEAQGKMVISRAVYLFHIGVNDYQYPFST 187

Query: 195 DSAFLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSPGM----RDEDGG 254
           +S+  +S  +  Y++ V+GN +  IKEVY  GGRKFGFLN+    C+P      + + G 
Sbjct: 188 NSSIFQSSPQEIYVDFVVGNTTAVIKEVYKIGGRKFGFLNMGAYDCAPASLIIDQTKIGT 247

Query: 255 CLEELSAYTSVHNQRLVKLLKDLERQLKGFKYSLYDFSSSLTDRMENPFKYG 298
           C + ++   ++HN++L   L+ LER+L GFKY+L+D+ +SL+ RM NP KYG
Sbjct: 248 CFKPVTELINLHNEKLESGLRRLERELSGFKYALHDYHTSLSVRMNNPSKYG 297

BLAST of CmaCh01G000150 vs. TAIR 10
Match: AT5G40990.1 (GDSL lipase 1 )

HSP 1 Score: 260.4 bits (664), Expect = 1.8e-69
Identity = 135/288 (46.88%), Postives = 190/288 (65.97%), Query Frame = 0

Query: 16  LIFFYFFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGNNNYINTTTLDQANFWPYG 75
           + F  +   ISI     ++ N +    +A F+FGDS  DAGNNNYI+T +  ++N+WPYG
Sbjct: 9   ITFLAYTIIISIGSINCIDNNNLVTNQSALFVFGDSVFDAGNNNYIDTLSSVRSNYWPYG 68

Query: 76  QTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQP--GFHHYHNGANFASAGAGALSET 135
           QT F+ PTGR SDGRL+ DFIAE+A LPLIPP LQP  G   +  G NFAS GAGAL  T
Sbjct: 69  QTTFKSPTGRVSDGRLIPDFIAEYAWLPLIPPNLQPFNGNSQFAYGVNFASGGAGALVGT 128

Query: 136 FHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLMLSKAVYLFSVGTNDYMSLFLTDSAF 195
           F G VI L+TQ+  F++ V   L+ KLG AEG  ++S+AVYLF +G NDY   F T+S+ 
Sbjct: 129 FSGLVINLRTQLNNFKK-VEEMLRSKLGDAEGKRVISRAVYLFHIGLNDYQYPFTTNSSL 188

Query: 196 LKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSPGM----RDEDGGCLEE 255
            +S S  +Y++ V+GN++   KEVY+ GGRKFG LN  P  C+P      + +   C + 
Sbjct: 189 FQSISNEKYVDYVVGNMTDVFKEVYNLGGRKFGILNTGPYDCAPASLVIDQTKIRSCFQP 248

Query: 256 LSAYTSVHNQRLVKLLKDLERQLKGFKYSLYDFSSSLTDRMENPFKYG 298
           ++   ++HN++L+  L+ L  +L GFKY+L+D+ +SL++RM +P KYG
Sbjct: 249 VTELINMHNEKLLNGLRRLNHELSGFKYALHDYHTSLSERMNDPSKYG 295

BLAST of CmaCh01G000150 vs. TAIR 10
Match: AT1G53990.1 (GDSL-motif lipase 3 )

HSP 1 Score: 252.7 bits (644), Expect = 3.9e-67
Identity = 135/290 (46.55%), Postives = 192/290 (66.21%), Query Frame = 0

Query: 14  LLLIFFYFFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGNNNYINTTTLDQANFWP 73
           +L+IFF +   +SI     ++ N +    AA F+FGDS  DAGNNNYINT +  ++N WP
Sbjct: 5   VLIIFFVYTIILSIGSINCIDNNNLVTNQAALFVFGDSLFDAGNNNYINTVSSFRSNIWP 64

Query: 74  YGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQP--GFHHYHNGANFASAGAGALS 133
           YGQT+F+FPTGR SDG        E A LP IPP LQP  G + +  G +FASAGAGAL+
Sbjct: 65  YGQTNFKFPTGRLSDG-------PEKAWLPSIPPNLQPNNGNNQFTYGVSFASAGAGALA 124

Query: 134 ETFHGSVIELKTQIKYFREVVVTWLKRKLGKAEGGLMLSKAVYLFSVGTNDYMSLFLTDS 193
           E+F G VI L TQ+  F++V  + L+ +LG AE   + S+AVYLF +G NDY   F  +S
Sbjct: 125 ESFLGMVINLGTQLNNFKDVEKS-LRSELGDAETKRVFSRAVYLFHIGANDYFYPFSANS 184

Query: 194 AFLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSPGM----RDEDGGCL 253
           +  KS+SK ++++ VIGN++  I+EVY  GGRKFGFLN+ P  CSP      R + G C 
Sbjct: 185 STFKSNSKEKFVDFVIGNITFVIEEVYKMGGRKFGFLNVGPYECSPNSLIRDRTKIGSCF 244

Query: 254 EELSAYTSVHNQRLVKLLKDLERQLKGFKYSLYDFSSSLTDRMENPFKYG 298
           + ++    +HN++   +L+ L+RQL GF+Y+L+D+ +SL++R+ +P KYG
Sbjct: 245 KPVAELIDMHNKKFPDVLRRLQRQLSGFRYALHDYHTSLSERINSPSKYG 286

BLAST of CmaCh01G000150 vs. TAIR 10
Match: AT3G14225.1 (GDSL-motif lipase 4 )

HSP 1 Score: 251.1 bits (640), Expect = 1.1e-66
Identity = 135/297 (45.45%), Postives = 191/297 (64.31%), Query Frame = 0

Query: 11  LIELLLIFFYFFNFISIYCFAEVEFNVVPEKHAAFFIFGDSFLDAGNNNYINTTTLDQAN 70
           +I +L I     + +SI C  +++ N      AA F FGDS  +AGNNNY ++ +  ++N
Sbjct: 9   IIIILFICTISLSIVSISCKEDLKTN-----QAALFAFGDSLFEAGNNNYFDSISSFRSN 68

Query: 71  FWPYGQTHFRFPTGRFSDGRLVSDFIAEFAKLPLIPPFLQPGFHHYH--NGANFASAGAG 130
           FWPYG+T F+FPTGR SDGR++ DFIAE+A LPLIPP LQPG+ +     G NFA+  AG
Sbjct: 69  FWPYGKTTFKFPTGRVSDGRIMIDFIAEYAWLPLIPPNLQPGYSNSQLTYGLNFATTAAG 128

Query: 131 ALSETFHGSVI----ELKTQIKYFREVVVTWLKRKLGKAEGGLMLSKAVYLFSVGTNDYM 190
             + TF GSV     +L TQ+  F+ V  T L+  LG AE   ++SKAVYLF +G NDY 
Sbjct: 129 VFAGTFPGSVTNLSKDLGTQLNNFKNVEKT-LRSNLGDAEARRVISKAVYLFHIGANDYQ 188

Query: 191 SLFLTDSAFLKSHSKSQYIELVIGNLSTSIKEVYDSGGRKFGFLNLPPIGCSPGM----R 250
             F  +++   + +K ++I+ VIGN +T I+E+Y  G RKFGFL+L P GC+P       
Sbjct: 189 YPFFANTSTFSNTTKERFIDFVIGNTTTVIEELYKLGARKFGFLSLGPFGCTPSALIINS 248

Query: 251 DEDGGCLEELSAYTSVHNQRLVKLLKDLERQLKGFKYSLYDFSSSLTDRMENPFKYG 298
            + G C E ++   ++HNQ   K+L+ LER+L GFKY+L+DF +SL+ R+ NP +YG
Sbjct: 249 TKIGSCFEPVTELINLHNQEFPKVLRRLERRLSGFKYALHDFHTSLSQRINNPSRYG 299

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SSA76.6e-8058.30GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2[more]
Q9SYF02.4e-6948.29GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1[more]
Q9FLN02.6e-6846.88GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1[more]
H6U1I85.8e-6847.27GDSL lipase OS=Tanacetum cinerariifolium OX=118510 GN=GLIP PE=1 SV=1[more]
Q9SYF55.4e-6646.55GDSL esterase/lipase 3 OS=Arabidopsis thaliana OX=3702 GN=GLIP3 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1KBX91.9e-170100.00GDSL esterase/lipase 5-like OS=Cucurbita maxima OX=3661 GN=LOC111492922 PE=3 SV=... [more]
A0A6J1GAV11.5e-16496.00GDSL esterase/lipase 5-like OS=Cucurbita moschata OX=3662 GN=LOC111452496 PE=3 S... [more]
A0A5D3D6J23.0e-13682.15GDSL esterase/lipase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold5... [more]
A0A1S3CCC43.0e-13682.15GDSL esterase/lipase 5 OS=Cucumis melo OX=3656 GN=LOC103499192 PE=3 SV=1[more]
A0A6J1GY702.0e-13581.40GDSL esterase/lipase 5-like OS=Cucurbita moschata OX=3662 GN=LOC111457918 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_022998205.13.9e-170100.00GDSL esterase/lipase 5-like [Cucurbita maxima][more]
XP_022949031.13.2e-16496.00GDSL esterase/lipase 5-like [Cucurbita moschata][more]
KAG6606674.11.2e-16396.01GDSL esterase/lipase 5, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023525118.11.7e-16295.00GDSL esterase/lipase 5-like [Cucurbita pepo subsp. pepo][more]
XP_038904622.11.9e-13780.53GDSL esterase/lipase 5 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G53920.14.7e-8158.30GDSL-motif lipase 5 [more]
AT1G53940.11.7e-7048.29GDSL-motif lipase 2 [more]
AT5G40990.11.8e-6946.88GDSL lipase 1 [more]
AT1G53990.13.9e-6746.55GDSL-motif lipase 3 [more]
AT3G14225.11.1e-6645.45GDSL-motif lipase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 46..289
e-value: 4.1E-22
score: 79.7
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 37..308
e-value: 2.1E-55
score: 190.4
NoneNo IPR availablePANTHERPTHR45966:SF4GDSL ESTERASE/LIPASE 5coord: 16..300
IPR044552GDSL esterase/lipase GLIP1-5/GLL25PANTHERPTHR45966GDSL-LIKE LIPASE/ACYLHYDROLASEcoord: 16..300
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 44..304
e-value: 7.68811E-93
score: 276.419

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G000150.1CmaCh01G000150.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016298 lipase activity
molecular_function GO:0016788 hydrolase activity, acting on ester bonds