CmaCh00G002960 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh00G002960
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionCCHC-type domain-containing protein
LocationCma_Chr00: 22643440 .. 22645542 (+)
RNA-Seq ExpressionCmaCh00G002960
SyntenyCmaCh00G002960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACAATGAAACCCGACGGAGAATCGAGGAAACGGTGATTGACTTATTGAAGATATCGAACATGGAAGAGATGACGGAGTACAAAATTCGAGCCGAGGCCGAAAAACGACTCGGAATGGATCTCTCCGATATTCAATGCAAGTGCCTGGTGAGGGACGTGGTCGAGGACTTTTTACATTCATTTACGGAACGTGATGATAAGGGCAAAGAGGGAGAACCAGGGCCTAGCGATCGTTACGAGAATAAAGCGACGGAGCAGGAGATAGTCCGGAAGAAGGAGATTAACGCTGATGTTGACCGTGTGATTTGCCAGGTAAAGCCTTTGCCCTTCTTGATTCTTGAGAAAAGTTGTTGTGAATTTGAAAGCGGAAAAATTGGGATTTTGCGTATATCTGGTGAACTTAGTGAATTGAACTTCAATGCAATGGAGCTTGTTGCCCTTCTTAGAATGCTTCCGAAGCTGGATGTATATGTTCCTATGGCTAGATTGTTATAATTCATTGCTCGTGATCATTATACTATAGTCTTTTGTTACTGAACTGCACCAAAAGGTTAGATTGTGGATGAAATTCCGGTGTCATTATACTATAGTCTTTTGTTACTGAACTGCACCAAAAGGTTAGATTGTGGATGAAATTCCGGTGTACATTCTCTGGCATTTGTGCATTATGATGTAAATTATTGAATCCTCTATATTTATGGTTGTAATAATTAAAGAAATGTTCATTTAGACAAGATCTTTACAGCACTGTATCCTTTCAAAGAAGTAGTTTAATAATTAAAGAAATGTTCATTTAGACAAGATCTTTACAGCACTGTATCCTTTCAAAGAAGTAGTTTTTGTTTTTAGAATTTGACAAAAAAAATTCAAATATTTATCCAACCTAATTAAAAATTATAGTAACGAAATTATGTATAAATTTCAAAAACAGAAAGGTAAATTATCAAATAGAGACAGTTGTTAAAATTGTCCATTACAGATTTTACTCTGTATGAAATATGATCCCCATATAATAACCCGTCTGCAATATTATGATTTAGTAGCTAATGATTTTCGGCTTTCTTTCAGCTATCTAATAACAGGAATGTGACAGTTCATGAATTTAAAGGGAACGCTCTGGTATCAATTAGGCAGTATTATGAAAAAGATGGAAAACAGCTTCCTGGTATTAAAGGTGGGCACTATGCATTGGTCTCTTCATTTCCTCTTATGGTGAATCTCCTTATTTTGCCCTGACACACCCTAACACATTTGGCTGAGGTGATGTGTTGGAAAATTTCTGGGAACTCGATTCTCTTGTATCATGTGAGTTCTGTTAACTTCACTTACCTTCCCCCTCCCCCTCACCTGTTCTCTTTTGACCCTTTACTTTATGGCAGGGGTGGGTTGAGAGTGTCTTGCTTCTATTATGAACTCTAACCATGTGAAACGTGATTAGTTAAACCAGTATTTAATATGATTCCAGCTGCTGTTGATTTTGATTTAGACCTGTTGGGTTAGGAGTTTCTTTTGATTGGGTCTGAACGACCAATCCATCTCGTAGTCCTATAAGCCCCCCTGTTTTTATTGAAATTATTATTCATGTTAGTTTTTGTCCATATTGATAATTTTAATTGCTAGGAATCAGCTTGACAACTGAACAATGGTCTGCCTTTAGGAGTAGTATTCCTGCTATAGAGGAAGCTATTTTGCAGATGAAAATGAAAATAAAAAGGTGAGAACCATGTTGATGTTCTTTGATACTACTGTTATTGAACCTGCTTGTTATAGTTCATCAAATTTATTTCATTAACAAGAAAACTCCAAAGTTTTATGATATGTTTTCAGGAGTTGGTGTCCAAATGTAGATTCCAAAACTTTTATTAATGTTTTCTTTCGTTAAGTTTTCTTGTTGGAGGATTTAAATTTCAGGACAAAAGGTGTATAGTTGAAATAACTAACTATACCATATCTTGTAGATCTGAACACGATGCTAATACAAGTGGTGCTGTCTCTGTACCTGCTACTGGGTCTGCTCCAAAATTTCCATCTGAAACTATTCGGTTTGATGGAAAAAACTACCGGGTATGGGCATGCCAGATGGAGCTTTTGTCCATCTGA

mRNA sequence

ATGGACAATGAAACCCGACGGAGAATCGAGGAAACGGTGATTGACTTATTGAAGATATCGAACATGGAAGAGATGACGGAGTACAAAATTCGAGCCGAGGCCGAAAAACGACTCGGAATGGATCTCTCCGATATTCAATGCAAGTGCCTGGTGAGGGACGTGGTCGAGGACTTTTTACATTCATTTACGGAACGTGATGATAAGGGCAAAGAGGGAGAACCAGGGCCTAGCGATCGTTACGAGAATAAAGCGACGGAGCAGGAGATAGTCCGGAAGAAGGAGATTAACGCTGATGTTGACCGTGTGATTTGCCAGGTAAAGCCTTTGCCCTTCTTGATTCTTGAGAAAAGTTGTTGTGAATTTGAAAGCGGAAAAATTGGGATTTTGCGTATATCTGGTGAACTTAGTGAATTGAACTTCAATGCAATGGAGCTTGTTGCCCTTCTTAGAATGCTTCCGAAGCTGGATCTATCTAATAACAGGAATGTGACAGTTCATGAATTTAAAGGGAACGCTCTGGTATCAATTAGGCAGTATTATGAAAAAGATGGAAAACAGCTTCCTGGAATCAGCTTGACAACTGAACAATGGTCTGCCTTTAGGAGTAGTATTCCTGCTATAGAGGAAGCTATTTTGCAGATGAAAATGAAAATAAAAAGATCTGAACACGATGCTAATACAAGTGGTGCTGTCTCTGTACCTGCTACTGGGTCTGCTCCAAAATTTCCATCTGAAACTATTCGGTTTGATGGAAAAAACTACCGGGTATGGGCATGCCAGATGGAGCTTTTGTCCATCTGA

Coding sequence (CDS)

ATGGACAATGAAACCCGACGGAGAATCGAGGAAACGGTGATTGACTTATTGAAGATATCGAACATGGAAGAGATGACGGAGTACAAAATTCGAGCCGAGGCCGAAAAACGACTCGGAATGGATCTCTCCGATATTCAATGCAAGTGCCTGGTGAGGGACGTGGTCGAGGACTTTTTACATTCATTTACGGAACGTGATGATAAGGGCAAAGAGGGAGAACCAGGGCCTAGCGATCGTTACGAGAATAAAGCGACGGAGCAGGAGATAGTCCGGAAGAAGGAGATTAACGCTGATGTTGACCGTGTGATTTGCCAGGTAAAGCCTTTGCCCTTCTTGATTCTTGAGAAAAGTTGTTGTGAATTTGAAAGCGGAAAAATTGGGATTTTGCGTATATCTGGTGAACTTAGTGAATTGAACTTCAATGCAATGGAGCTTGTTGCCCTTCTTAGAATGCTTCCGAAGCTGGATCTATCTAATAACAGGAATGTGACAGTTCATGAATTTAAAGGGAACGCTCTGGTATCAATTAGGCAGTATTATGAAAAAGATGGAAAACAGCTTCCTGGAATCAGCTTGACAACTGAACAATGGTCTGCCTTTAGGAGTAGTATTCCTGCTATAGAGGAAGCTATTTTGCAGATGAAAATGAAAATAAAAAGATCTGAACACGATGCTAATACAAGTGGTGCTGTCTCTGTACCTGCTACTGGGTCTGCTCCAAAATTTCCATCTGAAACTATTCGGTTTGATGGAAAAAACTACCGGGTATGGGCATGCCAGATGGAGCTTTTGTCCATCTGA

Protein sequence

MDNETRRRIEETVIDLLKISNMEEMTEYKIRAEAEKRLGMDLSDIQCKCLVRDVVEDFLHSFTERDDKGKEGEPGPSDRYENKATEQEIVRKKEINADVDRVICQVKPLPFLILEKSCCEFESGKIGILRISGELSELNFNAMELVALLRMLPKLDLSNNRNVTVHEFKGNALVSIRQYYEKDGKQLPGISLTTEQWSAFRSSIPAIEEAILQMKMKIKRSEHDANTSGAVSVPATGSAPKFPSETIRFDGKNYRVWACQMELLSI
Homology
[Close]
BLAST of CmaCh00G002960 vs. ExPASy Swiss-Prot
Match: O65155 (RNA polymerase II transcriptional coactivator KELP OS=Arabidopsis thaliana OX=3702 GN=KELP PE=1 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 3.1e-25
Identity = 76/221 (34.39%), Postives = 115/221 (52.04%), Query Frame = 0 

Query: 1   MDNETRRRIEETVIDLLKISNMEEMTEYKIRAEAEKRLGMDLSDIQCKCLVRDVVEDFLH 60
           M+ ET+ +IE+TVI++L  S+M+E+TE+K+R  A ++L +DLS+   K  VR VVE FL 
Sbjct: 1   MEKETKEKIEKTVIEILSESDMKEITEFKVRKLASEKLAIDLSEKSHKAFVRSVVEKFLD 60

Query: 61  SFTERDDKGKEGEPGPSDRYENKATEQEIVRKKEINADVDRVICQVKPLPFLILEKSCCE 120
                +++ +E E    ++ E    +      KE + D D +IC+               
Sbjct: 61  -----EERAREYENSQVNKEEEDGDKDCGKGNKEFDDDGDLIICR--------------- 120

Query: 121 FESGKIGILRISGELSELNFNAMELVALLRMLPKLDLSNNRNVTVHEFKGNALVSIRQYY 180
                                               LS+ R VT+ EFKG +LVSIR+YY
Sbjct: 121 ------------------------------------LSDKRRVTIQEFKGKSLVSIREYY 165

Query: 181 EKDGKQLP---GISLTTEQWSAFRSSIPAIEEAILQMKMKI 219
           +KDGK+LP   GISLT EQWS F+ ++PAIE A+ +M+ ++
Sbjct: 181 KKDGKELPTSKGISLTDEQWSTFKKNMPAIENAVKKMESRV 165

[Close]
BLAST of CmaCh00G002960 vs. ExPASy Swiss-Prot
Match: O65154 (RNA polymerase II transcriptional coactivator KIWI OS=Arabidopsis thaliana OX=3702 GN=KIWI PE=1 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 4.2e-06
Identity = 25/59 (42.37%), Postives = 38/59 (64.41%), Query Frame = 0 

Query: 156 DLSNNRNVTVHEFKGNALVSIRQYYEKDGKQLP---GISLTTEQWSAFRSSIPAIEEAI 212
           ++S NR V+V  + G   + IR++Y KDGK LP   GISL+ +QW+  R+    IE+A+
Sbjct: 45  NISKNRRVSVRNWNGKIWIDIREFYVKDGKTLPGKKGISLSVDQWNTLRNHAEDIEKAL 103

[Close]
BLAST of CmaCh00G002960 vs. ExPASy Swiss-Prot
Match: Q9FHX8 (Mediator-associated protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g42060 PE=1 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 1.0e-04
Identity = 25/59 (42.37%), Postives = 41/59 (69.49%), Query Frame = 0 

Query: 1  MDNETRRRIEETVIDLLKISNMEEMTEYKIRAEAEKRLGMDLSDIQCKCLVRDVVEDFL 60
          +D + RR+I++TV  +L+ SN+ ++TE K R EA  +L +DLS    K +V++ VE+FL
Sbjct: 11 IDKDLRRKIKKTVKKILESSNLYKITEIKAREEASLKLDLDLSQDPYKVIVKEEVENFL 69

[Close]
BLAST of CmaCh00G002960 vs. ExPASy Swiss-Prot
Match: P87294 (Putative RNA polymerase II transcriptional coactivator OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC16A10.02 PE=1 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.8e-04
Identity = 21/60 (35.00%), Postives = 36/60 (60.00%), Query Frame = 0 

Query: 155 LDLSNNRNVTVHEFKGNALVSIRQYYEKDGKQLP---GISLTTEQWSAFRSSIPAIEEAI 212
           L+ +  + +T+ EF+G   V IR+YYEKDG  LP   GI+L   +W   +  I  +++++
Sbjct: 32  LNETEKKRITLSEFRGTRYVHIREYYEKDGDMLPGKKGIALNINEWKKLKQLIHEVDDSL 91

[Close]
BLAST of CmaCh00G002960 vs. ExPASy Swiss-Prot
Match: P53999 (Activated RNA polymerase II transcriptional coactivator p15 OS=Homo sapiens OX=9606 GN=SUB1 PE=1 SV=3)

HSP 1 Score: 46.2 bits (108), Expect = 6.7e-04
Identity = 23/58 (39.66%), Postives = 37/58 (63.79%), Query Frame = 0 

Query: 161 RNVTVHEFKGNALVSIRQYY-EKDGKQLP---GISLTTEQWSAFRSSIPAIEEAILQM 215
           R V+V +FKG  L+ IR+Y+ + +G+  P   GISL  EQWS  +  I  I++A+ ++
Sbjct: 70  RYVSVRDFKGKVLIDIREYWMDPEGEMKPGRKGISLNPEQWSQLKEQISDIDDAVRKL 127

[Close]
BLAST of CmaCh00G002960 vs. TAIR 10
Match: AT4G10920.1 (transcriptional coactivator p15 (PC4) family protein (KELP) )

HSP 1 Score: 117.1 bits (292), Expect = 2.2e-26
Identity = 76/221 (34.39%), Postives = 115/221 (52.04%), Query Frame = 0 

Query: 1   MDNETRRRIEETVIDLLKISNMEEMTEYKIRAEAEKRLGMDLSDIQCKCLVRDVVEDFLH 60
           M+ ET+ +IE+TVI++L  S+M+E+TE+K+R  A ++L +DLS+   K  VR VVE FL 
Sbjct: 1   MEKETKEKIEKTVIEILSESDMKEITEFKVRKLASEKLAIDLSEKSHKAFVRSVVEKFLD 60

Query: 61  SFTERDDKGKEGEPGPSDRYENKATEQEIVRKKEINADVDRVICQVKPLPFLILEKSCCE 120
                +++ +E E    ++ E    +      KE + D D +IC+               
Sbjct: 61  -----EERAREYENSQVNKEEEDGDKDCGKGNKEFDDDGDLIICR--------------- 120

Query: 121 FESGKIGILRISGELSELNFNAMELVALLRMLPKLDLSNNRNVTVHEFKGNALVSIRQYY 180
                                               LS+ R VT+ EFKG +LVSIR+YY
Sbjct: 121 ------------------------------------LSDKRRVTIQEFKGKSLVSIREYY 165

Query: 181 EKDGKQLP---GISLTTEQWSAFRSSIPAIEEAILQMKMKI 219
           +KDGK+LP   GISLT EQWS F+ ++PAIE A+ +M+ ++
Sbjct: 181 KKDGKELPTSKGISLTDEQWSTFKKNMPAIENAVKKMESRV 165

[Close]
BLAST of CmaCh00G002960 vs. TAIR 10
Match: AT4G10920.2 (transcriptional coactivator p15 (PC4) family protein (KELP) )

HSP 1 Score: 117.1 bits (292), Expect = 2.2e-26
Identity = 76/221 (34.39%), Postives = 115/221 (52.04%), Query Frame = 0 

Query: 1   MDNETRRRIEETVIDLLKISNMEEMTEYKIRAEAEKRLGMDLSDIQCKCLVRDVVEDFLH 60
           M+ ET+ +IE+TVI++L  S+M+E+TE+K+R  A ++L +DLS+   K  VR VVE FL 
Sbjct: 1   MEKETKEKIEKTVIEILSESDMKEITEFKVRKLASEKLAIDLSEKSHKAFVRSVVEKFLD 60

Query: 61  SFTERDDKGKEGEPGPSDRYENKATEQEIVRKKEINADVDRVICQVKPLPFLILEKSCCE 120
                +++ +E E    ++ E    +      KE + D D +IC+               
Sbjct: 61  -----EERAREYENSQVNKEEEDGDKDCGKGNKEFDDDGDLIICR--------------- 120

Query: 121 FESGKIGILRISGELSELNFNAMELVALLRMLPKLDLSNNRNVTVHEFKGNALVSIRQYY 180
                                               LS+ R VT+ EFKG +LVSIR+YY
Sbjct: 121 ------------------------------------LSDKRRVTIQEFKGKSLVSIREYY 165

Query: 181 EKDGKQLP---GISLTTEQWSAFRSSIPAIEEAILQMKMKI 219
           +KDGK+LP   GISLT EQWS F+ ++PAIE A+ +M+ ++
Sbjct: 181 KKDGKELPTSKGISLTDEQWSTFKKNMPAIENAVKKMESRV 165

[Close]
BLAST of CmaCh00G002960 vs. TAIR 10
Match: AT4G00980.1 (zinc knuckle (CCHC-type) family protein )

HSP 1 Score: 90.9 bits (224), Expect = 1.7e-18
Identity = 77/263 (29.28%), Postives = 111/263 (42.21%), Query Frame = 0 

Query: 1   MDNETRRRIEETVIDLLKISNMEEMTEYKIRAEAEKRLGMDLSDIQCKCLVRDVVEDFLH 60
           M+    ++IEETV  +L  S+M++MTE+K+R +A  +LG+DLS    K LVRDV+E FL 
Sbjct: 12  MEIVATQKIEETVKSILSESDMDQMTEFKLRLDASAKLGIDLSGTNHKKLVRDVLEVFLL 71

Query: 61  SFTERDDKGKEGEPGPSDRYENKATEQEIVRKKEINADVDRVICQVKPLPFLILEKSCCE 120
           S          GE    +       E   V    +  + +R IC+               
Sbjct: 72  S--------TPGEALVPETVAPAKNETVSVAAASVGGEDERFICK--------------- 131

Query: 121 FESGKIGILRISGELSELNFNAMELVALLRMLPKLDLSNNRNVTVHEFKGNALVSIRQYY 180
                                               LS  +N TV  ++G   +SI    
Sbjct: 132 ------------------------------------LSEKQNATVQRYRGQPFLSIGS-- 191

Query: 181 EKDGKQLPGISLTTEQWSAFRSSIPAIEEAILQMKMKIKRSEHDANTSGAVSVPATGSAP 240
           ++ GK   G  L+T QWS  + +  AIE+ I Q + K+K SE   N   + +V    S  
Sbjct: 192 QEHGKAFRGAHLSTNQWSVIKKNFAAIEDGIKQCQSKLK-SEAARNGDTSEAVDKDSSHG 212

Query: 241 KFPSETIRFDGKNYRVWACQMEL 264
               +  RFDGK+Y  WA QMEL
Sbjct: 252 FSVIKISRFDGKSYLYWASQMEL 212

[Close]
BLAST of CmaCh00G002960 vs. TAIR 10
Match: AT5G09250.1 (ssDNA-binding transcriptional regulator )

HSP 1 Score: 53.5 bits (127), Expect = 3.0e-07
Identity = 25/59 (42.37%), Postives = 38/59 (64.41%), Query Frame = 0 

Query: 156 DLSNNRNVTVHEFKGNALVSIRQYYEKDGKQLP---GISLTTEQWSAFRSSIPAIEEAI 212
           ++S NR V+V  + G   + IR++Y KDGK LP   GISL+ +QW+  R+    IE+A+
Sbjct: 45  NISKNRRVSVRNWNGKIWIDIREFYVKDGKTLPGKKGISLSVDQWNTLRNHAEDIEKAL 103

[Close]
BLAST of CmaCh00G002960 vs. TAIR 10
Match: AT1G64490.1 (DEK, chromatin associated protein )

HSP 1 Score: 50.1 bits (118), Expect = 3.3e-06
Identity = 25/59 (42.37%), Postives = 40/59 (67.80%), Query Frame = 0 

Query: 1  MDNETRRRIEETVIDLLKISNMEEMTEYKIRAEAEKRLGMDLSDIQCKCLVRDVVEDFL 60
          +DN+ +++I+ETV  +LK S++ E+TE K R EA   L +DLS    K +VR+ V+ F+
Sbjct: 11 IDNDLKKKIKETVKKILKRSSLLEITEIKAREEASSELNLDLSRDPYKIIVREAVDSFI 69

The following BLAST results are available for this feature:
BLAST of CmaCh00G002960 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O651553.1e-2534.39RNA polymerase II transcriptional coactivator KELP OS=Arabidopsis thaliana OX=37... [more]
O651544.2e-0642.37RNA polymerase II transcriptional coactivator KIWI OS=Arabidopsis thaliana OX=37... [more]
Q9FHX81.0e-0442.37Mediator-associated protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g42060 PE=1 ... [more]
P872941.8e-0435.00Putative RNA polymerase II transcriptional coactivator OS=Schizosaccharomyces po... [more]
P539996.7e-0439.66Activated RNA polymerase II transcriptional coactivator p15 OS=Homo sapiens OX=9... [more]
BLAST of CmaCh00G002960 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G10920.12.2e-2634.39transcriptional coactivator p15 (PC4) family protein (KELP) [more]
AT4G10920.22.2e-2634.39transcriptional coactivator p15 (PC4) family protein (KELP) [more]
AT4G00980.11.7e-1829.28zinc knuckle (CCHC-type) family protein [more]
AT5G09250.13.0e-0742.37ssDNA-binding transcriptional regulator [more]
AT1G64490.13.3e-0642.37DEK, chromatin associated protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009044ssDNA-binding transcriptional regulatorGENE3D2.30.31.10Transcriptional Coactivator Pc4; Chain Acoord: 151..220
e-value: 2.2E-21
score: 77.2
IPR009044ssDNA-binding transcriptional regulatorSUPERFAMILY54447ssDNA-binding transcriptional regulator domaincoord: 155..212
IPR003173Transcriptional coactivator p15 (PC4), C-terminalPFAMPF02229PC4coord: 156..202
e-value: 3.9E-17
score: 61.6
IPR014876DEK, C-terminalPFAMPF08766DEK_Ccoord: 5..59
e-value: 7.9E-9
score: 35.3
NoneNo IPR availablePANTHERPTHR13215:SF6RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATOR KELPcoord: 3..106
NoneNo IPR availablePANTHERPTHR13215:SF6RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATOR KELPcoord: 157..216
IPR045125RNA polymerase II transcriptional coactivator Sub1/Tcp4-likePANTHERPTHR13215RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATORcoord: 157..216
coord: 3..106

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh00G002960.1CmaCh00G002960.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0060261 positive regulation of transcription initiation from RNA polymerase II promoter
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0003713 transcription coactivator activity
molecular_function GO:0008270 zinc ion binding