CmaCh00G001100 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh00G001100
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionChloroplast envelope membrane protein
LocationCma_Chr00: 4865437 .. 4865813 (-)
RNA-Seq ExpressionCmaCh00G001100
SyntenyCmaCh00G001100
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACGAGGATCGTATCCATACGATTTTGCACTTCTCCACAAATAGAATCCGTTTCATTATTCTAAGTGGTTATTCTCTATTCTTGGTAATTAAGAACTTGTTATTCTTCACTCTTGGGTTCACGAGTTCCTATATAATTTAAGCGACACAATCAAAGCTTTTTCTATTCTTTTATTAACTGATTTATGTTAAGGTATAGGATTCCATTCGCCCCATGGTTGGGAACTAATGATTGGTTCTGTCTACAAAGATTTTGGATTTTCTCATAACGATCTAATTCTATCCAGTCTTGTTTCCACTTTGTCGGTCATTCTGGACACAACAAATATTGGATCGTTATTTAAATCGTCTATCTCCGTCACTTGTAGTGATTGA

mRNA sequence

ATGAACGAGGATCGTATCCATACGATTTTGCACTTCTCCACAAATAGAATCCGTTTCATTATTCTAAGTGGTTATTCTCTATTCTTGGTAATTAAGAACTTGTTATTCTTCACTCTTGGGTTCACGAGTATAGGATTCCATTCGCCCCATGGTTGGGAACTAATGATTGGTTCTGTCTACAAAGATTTTGGATTTTCTCATAACGATCTAATTCTATCCAGTCTTGTTTCCACTTTGTCGGTCATTCTGGACACAACAAATATTGGATCGTTATTTAAATCGTCTATCTCCGTCACTTGTAGTGATTGA

Coding sequence (CDS)

ATGAACGAGGATCGTATCCATACGATTTTGCACTTCTCCACAAATAGAATCCGTTTCATTATTCTAAGTGGTTATTCTCTATTCTTGGTAATTAAGAACTTGTTATTCTTCACTCTTGGGTTCACGAGTATAGGATTCCATTCGCCCCATGGTTGGGAACTAATGATTGGTTCTGTCTACAAAGATTTTGGATTTTCTCATAACGATCTAATTCTATCCAGTCTTGTTTCCACTTTGTCGGTCATTCTGGACACAACAAATATTGGATCGTTATTTAAATCGTCTATCTCCGTCACTTGTAGTGATTGA

Protein sequence

MNEDRIHTILHFSTNRIRFIILSGYSLFLVIKNLLFFTLGFTSIGFHSPHGWELMIGSVYKDFGFSHNDLILSSLVSTLSVILDTTNIGSLFKSSISVTCSD
Homology
BLAST of CmaCh00G001100 vs. ExPASy Swiss-Prot
Match: Q2QD77 (Chloroplast envelope membrane protein OS=Cucumis sativus OX=3659 GN=cemA PE=3 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 2.5e-23
Identity = 65/104 (62.50%), Postives = 68/104 (65.38%), Query Frame = 0

Query: 2   NEDRIHTILHFSTNRIRFIILSGYSL---------------FL-----VIKNLLFFTLGF 61
           NEDRIHTILHFSTN I F+ILSGYS+               FL      IK      L  
Sbjct: 100 NEDRIHTILHFSTNIICFVILSGYSILGNEELVILNSWVQEFLYNLSDTIKAFSILLLTD 159

Query: 62  TSIGFHSPHGWELMIGSVYKDFGFSHNDLILSSLVSTLSVILDT 86
             IGFHSPHGWELMIGSVYKDFGFSHND I+SSLVST  VILDT
Sbjct: 160 LCIGFHSPHGWELMIGSVYKDFGFSHNDQIISSLVSTFPVILDT 203

BLAST of CmaCh00G001100 vs. ExPASy Swiss-Prot
Match: B1NWG2 (Chloroplast envelope membrane protein OS=Manihot esculenta OX=3983 GN=cemA PE=3 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 1.2e-22
Identity = 64/104 (61.54%), Postives = 67/104 (64.42%), Query Frame = 0

Query: 2   NEDRIHTILHFSTNRIRFIILSGYSL---------------FL-----VIKNLLFFTLGF 61
           NEDRIHTILHFSTN I FIILSGYS+               FL      IK      L  
Sbjct: 102 NEDRIHTILHFSTNIICFIILSGYSILSNEELIILNSWVQEFLYNLSDTIKAFSILLLTD 161

Query: 62  TSIGFHSPHGWELMIGSVYKDFGFSHNDLILSSLVSTLSVILDT 86
             IGFHSPHGWELMIGSVYKDFGF+HND I+S LVST  VILDT
Sbjct: 162 LCIGFHSPHGWELMIGSVYKDFGFAHNDQIISGLVSTFPVILDT 205

BLAST of CmaCh00G001100 vs. ExPASy Swiss-Prot
Match: Q68RZ4 (Chloroplast envelope membrane protein OS=Panax ginseng OX=4054 GN=cemA PE=3 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 4.6e-22
Identity = 64/104 (61.54%), Postives = 69/104 (66.35%), Query Frame = 0

Query: 2   NEDRIHTILHFSTNRIRFIILSGYSLF----LVIKNL----LFFTLGFT----------- 61
           NEDRIHTILHFSTN I F+ILSGYS++    LVI N       + L  T           
Sbjct: 100 NEDRIHTILHFSTNIICFVILSGYSIWGNEELVILNSWAQEFLYNLSDTIKAFSILLLTD 159

Query: 62  -SIGFHSPHGWELMIGSVYKDFGFSHNDLILSSLVSTLSVILDT 86
             IGFHSPHGWEL+IGSVYKDFGF HND ILS LVST  VILDT
Sbjct: 160 LCIGFHSPHGWELIIGSVYKDFGFVHNDQILSGLVSTFPVILDT 203

BLAST of CmaCh00G001100 vs. ExPASy Swiss-Prot
Match: A0ZZ47 (Chloroplast envelope membrane protein OS=Gossypium barbadense OX=3634 GN=cemA PE=3 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 6.1e-22
Identity = 61/104 (58.65%), Postives = 66/104 (63.46%), Query Frame = 0

Query: 2   NEDRIHTILHFSTNRIRFIILSGYSL---------------FL-----VIKNLLFFTLGF 61
           NED IHTILHFSTN I F+ILSGYS+               FL      IK      L  
Sbjct: 100 NEDHIHTILHFSTNLICFVILSGYSILGNEELLILNSWAQEFLYNLSDTIKAFSILLLTD 159

Query: 62  TSIGFHSPHGWELMIGSVYKDFGFSHNDLILSSLVSTLSVILDT 86
             IGFHSPHGWELMIGS+YKDFGF+HND I+S LVST  VILDT
Sbjct: 160 LCIGFHSPHGWELMIGSIYKDFGFTHNDQIISGLVSTFPVILDT 203

BLAST of CmaCh00G001100 vs. ExPASy Swiss-Prot
Match: Q2L922 (Chloroplast envelope membrane protein OS=Gossypium hirsutum OX=3635 GN=cemA PE=3 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 6.1e-22
Identity = 61/104 (58.65%), Postives = 66/104 (63.46%), Query Frame = 0

Query: 2   NEDRIHTILHFSTNRIRFIILSGYSL---------------FL-----VIKNLLFFTLGF 61
           NED IHTILHFSTN I F+ILSGYS+               FL      IK      L  
Sbjct: 100 NEDHIHTILHFSTNLICFVILSGYSILGNEELLILNSWVQEFLYNLSDTIKAFSILLLTD 159

Query: 62  TSIGFHSPHGWELMIGSVYKDFGFSHNDLILSSLVSTLSVILDT 86
             IGFHSPHGWELMIGS+YKDFGF+HND I+S LVST  VILDT
Sbjct: 160 LCIGFHSPHGWELMIGSIYKDFGFAHNDQIISGLVSTFPVILDT 203

BLAST of CmaCh00G001100 vs. ExPASy TrEMBL
Match: A0A251VFK2 (Putative chloroplast envelope membrane protein, CemA OS=Helianthus annuus OX=4232 GN=HannXRQ_Chr02g0044891 PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 3.1e-21
Identity = 73/123 (59.35%), Postives = 75/123 (60.98%), Query Frame = 0

Query: 2   NEDRIHTILHFSTNRIRFIILSGYSL---------------FL-----VIKNLLFFTLGF 61
           NE RIHTILHFSTN I FIILSGYS+               FL      IK      L  
Sbjct: 29  NEGRIHTILHFSTNIICFIILSGYSILGNKELVILNSWAQEFLYNLSDTIKAFSLLLLTD 88

Query: 62  TSIGFHSPHGWELMIGSVYKDFGFSHNDLILSSLVSTLSVILDT--TNIGSLFKSSISVT 103
             IGFHSPHGWELMIG VYKDFGF HND I+S LVST  VILDT       LFKS ISVT
Sbjct: 89  LCIGFHSPHGWELMIGFVYKDFGFVHNDQIISGLVSTFPVILDTILKYDFPLFKSCISVT 148

BLAST of CmaCh00G001100 vs. ExPASy TrEMBL
Match: A0A7D3UX49 (envelope membrane protein, chloroplastic OS=Alchemilla argyrophylla OX=478290 GN=cemA PE=3 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 5.3e-21
Identity = 67/104 (64.42%), Postives = 70/104 (67.31%), Query Frame = 0

Query: 2   NEDRIHTILHFSTNRIRFIILSGYSLF----LVIKN--------------LLFFTLGFTS 61
           NEDRIHTILHFSTN I F+ILSGYS+     LVI N                FF L  T 
Sbjct: 100 NEDRIHTILHFSTNIICFVILSGYSILGNEELVILNSWVQEFLYNLSDTIKAFFILLLTD 159

Query: 62  --IGFHSPHGWELMIGSVYKDFGFSHNDLILSSLVSTLSVILDT 86
             IGFHSPHGWELMIGSVYKDFGFSHND I+S LVST  VILDT
Sbjct: 160 LCIGFHSPHGWELMIGSVYKDFGFSHNDEIISGLVSTFPVILDT 203

BLAST of CmaCh00G001100 vs. ExPASy TrEMBL
Match: A0A1V0ILF0 (Chloroplast envelope membrane protein OS=Alchemilla pectinata OX=478367 GN=cemA PE=3 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 5.3e-21
Identity = 67/104 (64.42%), Postives = 70/104 (67.31%), Query Frame = 0

Query: 2   NEDRIHTILHFSTNRIRFIILSGYSLF----LVIKN--------------LLFFTLGFTS 61
           NEDRIHTILHFSTN I F+ILSGYS+     LVI N                FF L  T 
Sbjct: 100 NEDRIHTILHFSTNIICFVILSGYSILGNEELVILNSWVQEFLYNLSDTIKAFFILLLTD 159

Query: 62  --IGFHSPHGWELMIGSVYKDFGFSHNDLILSSLVSTLSVILDT 86
             IGFHSPHGWELMIGSVYKDFGFSHND I+S LVST  VILDT
Sbjct: 160 LCIGFHSPHGWELMIGSVYKDFGFSHNDEIISGLVSTFPVILDT 203

BLAST of CmaCh00G001100 vs. ExPASy TrEMBL
Match: A0A7D3QBW5 (envelope membrane protein, chloroplastic OS=Alchemilla pedata OX=478332 GN=cemA PE=3 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 5.3e-21
Identity = 67/104 (64.42%), Postives = 70/104 (67.31%), Query Frame = 0

Query: 2   NEDRIHTILHFSTNRIRFIILSGYSLF----LVIKN--------------LLFFTLGFTS 61
           NEDRIHTILHFSTN I F+ILSGYS+     LVI N                FF L  T 
Sbjct: 100 NEDRIHTILHFSTNIICFVILSGYSILGNEELVILNSWVQEFLYNLSDTIKAFFILLLTD 159

Query: 62  --IGFHSPHGWELMIGSVYKDFGFSHNDLILSSLVSTLSVILDT 86
             IGFHSPHGWELMIGSVYKDFGFSHND I+S LVST  VILDT
Sbjct: 160 LCIGFHSPHGWELMIGSVYKDFGFSHNDEIISGLVSTFPVILDT 203

BLAST of CmaCh00G001100 vs. ExPASy TrEMBL
Match: A0A1P8C7Q2 (envelope membrane protein, chloroplastic OS=Cucumis x hytivus OX=442738 GN=cemA PE=3 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 6.9e-21
Identity = 65/104 (62.50%), Postives = 68/104 (65.38%), Query Frame = 0

Query: 2   NEDRIHTILHFSTNRIRFIILSGYSL---------------FL-----VIKNLLFFTLGF 61
           NEDRIHTILHFSTN I F+ILSGYS+               FL      IK      L  
Sbjct: 100 NEDRIHTILHFSTNIICFVILSGYSILGNEELVILNSWVQEFLYNLSDTIKAFCILLLTD 159

Query: 62  TSIGFHSPHGWELMIGSVYKDFGFSHNDLILSSLVSTLSVILDT 86
             IGFHSPHGWELMIGSVYKDFGFSHND I+SSLVST  VILDT
Sbjct: 160 LCIGFHSPHGWELMIGSVYKDFGFSHNDQIISSLVSTFPVILDT 203

BLAST of CmaCh00G001100 vs. NCBI nr
Match: QXE41275.1 (chloroplast envelope membrane protein [Olea hainanensis])

HSP 1 Score: 110.5 bits (275), Expect = 8.4e-21
Identity = 66/104 (63.46%), Postives = 70/104 (67.31%), Query Frame = 0

Query: 2   NEDRIHTILHFSTNRIRFIILSGYSLF----LVIKNL----LFFTLGFT----------- 61
           NEDRIHTILHFSTNRI F+ILSGYS+F    LVI N       + L  T           
Sbjct: 100 NEDRIHTILHFSTNRICFVILSGYSVFGNEELVILNSWAQEFLYNLSDTVKAFSILLLTD 159

Query: 62  -SIGFHSPHGWELMIGSVYKDFGFSHNDLILSSLVSTLSVILDT 86
             IGFHSPHGWELMIGSVYKDFGF HND I+S LVST  VILDT
Sbjct: 160 LCIGFHSPHGWELMIGSVYKDFGFVHNDQIISGLVSTFPVILDT 203

BLAST of CmaCh00G001100 vs. NCBI nr
Match: ARC97284.1 (envelope membrane protein [Alchemilla pectinata])

HSP 1 Score: 110.2 bits (274), Expect = 1.1e-20
Identity = 67/104 (64.42%), Postives = 70/104 (67.31%), Query Frame = 0

Query: 2   NEDRIHTILHFSTNRIRFIILSGYSLF----LVIKN--------------LLFFTLGFTS 61
           NEDRIHTILHFSTN I F+ILSGYS+     LVI N                FF L  T 
Sbjct: 100 NEDRIHTILHFSTNIICFVILSGYSILGNEELVILNSWVQEFLYNLSDTIKAFFILLLTD 159

Query: 62  --IGFHSPHGWELMIGSVYKDFGFSHNDLILSSLVSTLSVILDT 86
             IGFHSPHGWELMIGSVYKDFGFSHND I+S LVST  VILDT
Sbjct: 160 LCIGFHSPHGWELMIGSVYKDFGFSHNDEIISGLVSTFPVILDT 203

BLAST of CmaCh00G001100 vs. NCBI nr
Match: YP_009865116.1 (envelope membrane protein [Alchemilla argyrophylla] >YP_009865203.1 envelope membrane protein [Alchemilla pedata] >QKE47186.1 envelope membrane protein [Alchemilla argyrophylla] >QKE47273.1 envelope membrane protein [Alchemilla pedata])

HSP 1 Score: 110.2 bits (274), Expect = 1.1e-20
Identity = 67/104 (64.42%), Postives = 70/104 (67.31%), Query Frame = 0

Query: 2   NEDRIHTILHFSTNRIRFIILSGYSLF----LVIKN--------------LLFFTLGFTS 61
           NEDRIHTILHFSTN I F+ILSGYS+     LVI N                FF L  T 
Sbjct: 100 NEDRIHTILHFSTNIICFVILSGYSILGNEELVILNSWVQEFLYNLSDTIKAFFILLLTD 159

Query: 62  --IGFHSPHGWELMIGSVYKDFGFSHNDLILSSLVSTLSVILDT 86
             IGFHSPHGWELMIGSVYKDFGFSHND I+S LVST  VILDT
Sbjct: 160 LCIGFHSPHGWELMIGSVYKDFGFSHNDEIISGLVSTFPVILDT 203

BLAST of CmaCh00G001100 vs. NCBI nr
Match: YP_009346496.1 (chloroplast envelope membrane protein [Cucumis x hytivus] >AOW71106.1 chloroplast envelope membrane protein [Cucumis x hytivus] >QCY72854.1 CemA [Cucumis hystrix])

HSP 1 Score: 109.8 bits (273), Expect = 1.4e-20
Identity = 65/104 (62.50%), Postives = 68/104 (65.38%), Query Frame = 0

Query: 2   NEDRIHTILHFSTNRIRFIILSGYSL---------------FL-----VIKNLLFFTLGF 61
           NEDRIHTILHFSTN I F+ILSGYS+               FL      IK      L  
Sbjct: 100 NEDRIHTILHFSTNIICFVILSGYSILGNEELVILNSWVQEFLYNLSDTIKAFCILLLTD 159

Query: 62  TSIGFHSPHGWELMIGSVYKDFGFSHNDLILSSLVSTLSVILDT 86
             IGFHSPHGWELMIGSVYKDFGFSHND I+SSLVST  VILDT
Sbjct: 160 LCIGFHSPHGWELMIGSVYKDFGFSHNDQIISSLVSTFPVILDT 203

BLAST of CmaCh00G001100 vs. NCBI nr
Match: YP_009004056.1 (chloroplast envelope membrane protein [Cucumis hystrix] >Q2QD77.1 RecName: Full=Chloroplast envelope membrane protein [Cucumis sativus] >ALF03313.1 envelope membrane protein [Cucumis sativus var. hardwickii] >AVE15343.1 CemA [Cucumis sativus var. sativus] >AAZ94663.1 potential heme-binding protein [Cucumis sativus] >ABI97429.1 chloroplast envelope membrane protein [Cucumis sativus] >ABI98757.1 chloroplast envelope membrane protein [Cucumis sativus])

HSP 1 Score: 109.4 bits (272), Expect = 1.9e-20
Identity = 65/104 (62.50%), Postives = 68/104 (65.38%), Query Frame = 0

Query: 2   NEDRIHTILHFSTNRIRFIILSGYSL---------------FL-----VIKNLLFFTLGF 61
           NEDRIHTILHFSTN I F+ILSGYS+               FL      IK      L  
Sbjct: 100 NEDRIHTILHFSTNIICFVILSGYSILGNEELVILNSWVQEFLYNLSDTIKAFSILLLTD 159

Query: 62  TSIGFHSPHGWELMIGSVYKDFGFSHNDLILSSLVSTLSVILDT 86
             IGFHSPHGWELMIGSVYKDFGFSHND I+SSLVST  VILDT
Sbjct: 160 LCIGFHSPHGWELMIGSVYKDFGFSHNDQIISSLVSTFPVILDT 203

BLAST of CmaCh00G001100 vs. TAIR 10
Match: ATCG00530.1 (CemA-like proton extrusion protein-related )

HSP 1 Score: 96.3 bits (238), Expect = 1.5e-20
Identity = 58/104 (55.77%), Postives = 64/104 (61.54%), Query Frame = 0

Query: 2   NEDRIHTILHFSTNRIRFIILSGYS------LFLV--------------IKNLLFFTLGF 61
           NE  IHTILHFSTN I F+ILSGYS      LF++              IK      L  
Sbjct: 100 NEYNIHTILHFSTNLISFVILSGYSFWGKEKLFILNSWVQEFLYNLSDTIKAFSILLLTD 159

Query: 62  TSIGFHSPHGWELMIGSVYKDFGFSHNDLILSSLVSTLSVILDT 86
             IGFHSPHGWELMIG +YKDFGF+H + ILS LVST  VILDT
Sbjct: 160 LCIGFHSPHGWELMIGYIYKDFGFAHYEQILSGLVSTFPVILDT 203

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q2QD772.5e-2362.50Chloroplast envelope membrane protein OS=Cucumis sativus OX=3659 GN=cemA PE=3 SV... [more]
B1NWG21.2e-2261.54Chloroplast envelope membrane protein OS=Manihot esculenta OX=3983 GN=cemA PE=3 ... [more]
Q68RZ44.6e-2261.54Chloroplast envelope membrane protein OS=Panax ginseng OX=4054 GN=cemA PE=3 SV=1[more]
A0ZZ476.1e-2258.65Chloroplast envelope membrane protein OS=Gossypium barbadense OX=3634 GN=cemA PE... [more]
Q2L9226.1e-2258.65Chloroplast envelope membrane protein OS=Gossypium hirsutum OX=3635 GN=cemA PE=3... [more]
Match NameE-valueIdentityDescription
A0A251VFK23.1e-2159.35Putative chloroplast envelope membrane protein, CemA OS=Helianthus annuus OX=423... [more]
A0A7D3UX495.3e-2164.42envelope membrane protein, chloroplastic OS=Alchemilla argyrophylla OX=478290 GN... [more]
A0A1V0ILF05.3e-2164.42Chloroplast envelope membrane protein OS=Alchemilla pectinata OX=478367 GN=cemA ... [more]
A0A7D3QBW55.3e-2164.42envelope membrane protein, chloroplastic OS=Alchemilla pedata OX=478332 GN=cemA ... [more]
A0A1P8C7Q26.9e-2162.50envelope membrane protein, chloroplastic OS=Cucumis x hytivus OX=442738 GN=cemA ... [more]
Match NameE-valueIdentityDescription
QXE41275.18.4e-2163.46chloroplast envelope membrane protein [Olea hainanensis][more]
ARC97284.11.1e-2064.42envelope membrane protein [Alchemilla pectinata][more]
YP_009865116.11.1e-2064.42envelope membrane protein [Alchemilla argyrophylla] >YP_009865203.1 envelope mem... [more]
YP_009346496.11.4e-2062.50chloroplast envelope membrane protein [Cucumis x hytivus] >AOW71106.1 chloroplas... [more]
YP_009004056.11.9e-2062.50chloroplast envelope membrane protein [Cucumis hystrix] >Q2QD77.1 RecName: Full=... [more]
Match NameE-valueIdentityDescription
ATCG00530.11.5e-2055.77CemA-like proton extrusion protein-related [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004282Chloroplast envelope membrane protein, CemAPFAMPF03040CemAcoord: 32..86
e-value: 2.2E-12
score: 47.4
IPR004282Chloroplast envelope membrane protein, CemAPANTHERPTHR33650CHLOROPLAST ENVELOPE MEMBRANE PROTEIN-RELATEDcoord: 2..85
NoneNo IPR availablePANTHERPTHR33650:SF2CHLOROPLAST ENVELOPE MEMBRANE PROTEINcoord: 2..85

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh00G001100.1CmaCh00G001100.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009536 plastid