CmaCh00G000070 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh00G000070
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionReverse transcriptase
LocationCma_Chr00: 548552 .. 549472 (-)
RNA-Seq ExpressionCmaCh00G000070
SyntenyCmaCh00G000070
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGGTCGTAGAGAGATTAACGAAGTCAGCACATTTCCTCCCCGGCAAGGCCACCTATATAGTGGACATGTGGCGCAACTGTACATGAAGGAAATCGTCAGGTTGCATGGGGTCCCCGTGTCGATTGTATCATACCGAGATCCACGGTTTACTTCGGCATTTTGGCGTGGACTCCAGAAGGCGTTGGGCACTCGACTGGACTTTAGCACAGCGTATCACCCACAGACAAATGGCCAAACGGAATGCTTAAACCAAGTTCTAGAGGATATGTTGCGGGCCCGTGTACTGGATTTCTCTGGGAGTTGGGACTCGATATTGCACCTTATGGAATTCACTTATAATAACAGCTACCAGGCAACAATCGGTATGGCACCGTACGAGGCATTGTATAGAAGGCGGTGTAGGACACCATTATGCTGGGGCGAGGTAGGAGAACGGGAACTGGTGGGTCCGGAGCTGGTCCAAGTCACGAACGAGGCGATGCAACAGATCAAGGGGAGGATGCGCACTGCTCAGAGTAGACAGAAGAGTTACGCGGACTCGAGACGAAGAAACCTGGAGTTCGAAGTGGGTGACATGGTATTCCTGAAGGTGGCCCCTATGAAGGGCGTTCTAAGGTTCGGACGCAAGGGCAAATTAAGTCCGCGCTTCATAGGACCCTTCGGGATTTTGGAGCGAATCGGACCCATTGAATATAGGTTAGCCCTATCCCCGCCTGTCTCGTCCATACACAACGTGTTCCATGTATCGACCCTTCGCAAGTATTTAGCGAACCCCACGCACGTGATAGATTATTCAACCCTGGACATGACCAAAGACTTAAGCTACGAGGAGAAACCAGTGGGGGTCCTAGCTCGCAAGGTAAAGACCTTGCGCACTAGGGAAATTGCATTTGTCAAGGTGTGGTGGGGGAACTAG

mRNA sequence

ATGGGTGGTCGTAGAGAGATTAACGAAGTCAGCACATTTCCTCCCCGGCAAGGCCACCTATATAGTGGACATGTGGCGCAACTGTACATGAAGGAAATCGTCAGGTTGCATGGGGTCCCCGTGTCGATTGTATCATACCGAGATCCACGGTTTACTTCGGCATTTTGGCGTGGACTCCAGAAGGCGTTGGGCACTCGACTGGACTTTAGCACAGCGTATCACCCACAGACAAATGGCCAAACGGAATGCTTAAACCAAGTTCTAGAGGATATGTTGCGGGCCCGTGTACTGGATTTCTCTGGGAGTTGGGACTCGATATTGCACCTTATGGAATTCACTTATAATAACAGCTACCAGGCAACAATCGGTATGGCACCGTACGAGGCATTGTATAGAAGGCGGTGTAGGACACCATTATGCTGGGGCGAGGTAGGAGAACGGGAACTGGTGGGTCCGGAGCTGGTCCAAGTCACGAACGAGGCGATGCAACAGATCAAGGGGAGGATGCGCACTGCTCAGAGTAGACAGAAGAGTTACGCGGACTCGAGACGAAGAAACCTGGAGTTCGAAGTGGGTGACATGGTATTCCTGAAGGTGGCCCCTATGAAGGGCGTTCTAAGGTTCGGACGCAAGGGCAAATTAAGTCCGCGCTTCATAGGACCCTTCGGGATTTTGGAGCGAATCGGACCCATTGAATATAGGTTAGCCCTATCCCCGCCTGTCTCGTCCATACACAACGTGTTCCATGTATCGACCCTTCGCAAGTATTTAGCGAACCCCACGCACGTGATAGATTATTCAACCCTGGACATGACCAAAGACTTAAGCTACGAGGAGAAACCAGTGGGGGTCCTAGCTCGCAAGGTAAAGACCTTGCGCACTAGGGAAATTGCATTTGTCAAGGTGTGGTGGGGGAACTAG

Coding sequence (CDS)

ATGGGTGGTCGTAGAGAGATTAACGAAGTCAGCACATTTCCTCCCCGGCAAGGCCACCTATATAGTGGACATGTGGCGCAACTGTACATGAAGGAAATCGTCAGGTTGCATGGGGTCCCCGTGTCGATTGTATCATACCGAGATCCACGGTTTACTTCGGCATTTTGGCGTGGACTCCAGAAGGCGTTGGGCACTCGACTGGACTTTAGCACAGCGTATCACCCACAGACAAATGGCCAAACGGAATGCTTAAACCAAGTTCTAGAGGATATGTTGCGGGCCCGTGTACTGGATTTCTCTGGGAGTTGGGACTCGATATTGCACCTTATGGAATTCACTTATAATAACAGCTACCAGGCAACAATCGGTATGGCACCGTACGAGGCATTGTATAGAAGGCGGTGTAGGACACCATTATGCTGGGGCGAGGTAGGAGAACGGGAACTGGTGGGTCCGGAGCTGGTCCAAGTCACGAACGAGGCGATGCAACAGATCAAGGGGAGGATGCGCACTGCTCAGAGTAGACAGAAGAGTTACGCGGACTCGAGACGAAGAAACCTGGAGTTCGAAGTGGGTGACATGGTATTCCTGAAGGTGGCCCCTATGAAGGGCGTTCTAAGGTTCGGACGCAAGGGCAAATTAAGTCCGCGCTTCATAGGACCCTTCGGGATTTTGGAGCGAATCGGACCCATTGAATATAGGTTAGCCCTATCCCCGCCTGTCTCGTCCATACACAACGTGTTCCATGTATCGACCCTTCGCAAGTATTTAGCGAACCCCACGCACGTGATAGATTATTCAACCCTGGACATGACCAAAGACTTAAGCTACGAGGAGAAACCAGTGGGGGTCCTAGCTCGCAAGGTAAAGACCTTGCGCACTAGGGAAATTGCATTTGTCAAGGTGTGGTGGGGGAACTAG

Protein sequence

MGGRREINEVSTFPPRQGHLYSGHVAQLYMKEIVRLHGVPVSIVSYRDPRFTSAFWRGLQKALGTRLDFSTAYHPQTNGQTECLNQVLEDMLRARVLDFSGSWDSILHLMEFTYNNSYQATIGMAPYEALYRRRCRTPLCWGEVGERELVGPELVQVTNEAMQQIKGRMRTAQSRQKSYADSRRRNLEFEVGDMVFLKVAPMKGVLRFGRKGKLSPRFIGPFGILERIGPIEYRLALSPPVSSIHNVFHVSTLRKYLANPTHVIDYSTLDMTKDLSYEEKPVGVLARKVKTLRTREIAFVKVWWGN
Homology
BLAST of CmaCh00G000070 vs. ExPASy Swiss-Prot
Match: Q9UR07 (Transposon Tf2-11 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-11 PE=3 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.6e-25
Identity = 79/280 (28.21%), Postives = 135/280 (48.21%), Query Frame = 0

Query: 26   AQLYMKEIVRLHGVPVSIVSYRDPRFTSAFWRGLQKALGTRLDFSTAYHPQTNGQTECLN 85
            A+++ + ++   G P  I++  D  FTS  W+         + FS  Y PQT+GQTE  N
Sbjct: 1030 ARMFDQRVIAYFGNPKEIIADNDHIFTSQTWKDFAHKYNFVMKFSLPYRPQTDGQTERTN 1089

Query: 86   QVLEDMLRARVLDFSGSWDSILHLMEFTYNNSYQATIGMAPYEALYR-RRCRTPLCWGEV 145
            Q +E +LR        +W   + L++ +YNN+  +   M P+E ++R     +PL     
Sbjct: 1090 QTVEKLLRCVCSTHPNTWVDHISLVQQSYNNAIHSATQMTPFEIVHRYSPALSPLELPSF 1149

Query: 146  GERELVGPELVQVTNEAMQQIKGRMRTAQSRQKSYADSRRRNL-EFEVGDMVFLKVAPMK 205
             ++     E  Q T +  Q +K  + T   + K Y D + + + EF+ GD+V +K     
Sbjct: 1150 SDKT---DENSQETIQVFQTVKEHLNTNNIKMKKYFDMKIQEIEEFQPGDLVMVK-RTKT 1209

Query: 206  GVLRFGRKGKLSPRFIGPFGILERIGPIEYRLALSPPVSSI-HNVFHVSTLRKYLANPTH 265
            G L   +  KL+P F GPF +L++ GP  Y L L   +  +  + FHVS L KY  N   
Sbjct: 1210 GFLH--KSNKLAPSFAGPFYVLQKSGPNNYELDLPDSIKHMFSSTFHVSHLEKYRHNSE- 1269

Query: 266  VIDYSTLDMTKDLSYEEKPVGVLARKVKTLRTREIAFVKV 303
             ++Y+T+D        E  +G +   ++     ++ ++ V
Sbjct: 1270 -LNYATID--------ESDIGTILHILEHKNREQVLYLNV 1293

BLAST of CmaCh00G000070 vs. ExPASy Swiss-Prot
Match: P0CT41 (Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-12 PE=3 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.6e-25
Identity = 79/280 (28.21%), Postives = 135/280 (48.21%), Query Frame = 0

Query: 26   AQLYMKEIVRLHGVPVSIVSYRDPRFTSAFWRGLQKALGTRLDFSTAYHPQTNGQTECLN 85
            A+++ + ++   G P  I++  D  FTS  W+         + FS  Y PQT+GQTE  N
Sbjct: 1030 ARMFDQRVIAYFGNPKEIIADNDHIFTSQTWKDFAHKYNFVMKFSLPYRPQTDGQTERTN 1089

Query: 86   QVLEDMLRARVLDFSGSWDSILHLMEFTYNNSYQATIGMAPYEALYR-RRCRTPLCWGEV 145
            Q +E +LR        +W   + L++ +YNN+  +   M P+E ++R     +PL     
Sbjct: 1090 QTVEKLLRCVCSTHPNTWVDHISLVQQSYNNAIHSATQMTPFEIVHRYSPALSPLELPSF 1149

Query: 146  GERELVGPELVQVTNEAMQQIKGRMRTAQSRQKSYADSRRRNL-EFEVGDMVFLKVAPMK 205
             ++     E  Q T +  Q +K  + T   + K Y D + + + EF+ GD+V +K     
Sbjct: 1150 SDKT---DENSQETIQVFQTVKEHLNTNNIKMKKYFDMKIQEIEEFQPGDLVMVK-RTKT 1209

Query: 206  GVLRFGRKGKLSPRFIGPFGILERIGPIEYRLALSPPVSSI-HNVFHVSTLRKYLANPTH 265
            G L   +  KL+P F GPF +L++ GP  Y L L   +  +  + FHVS L KY  N   
Sbjct: 1210 GFLH--KSNKLAPSFAGPFYVLQKSGPNNYELDLPDSIKHMFSSTFHVSHLEKYRHNSE- 1269

Query: 266  VIDYSTLDMTKDLSYEEKPVGVLARKVKTLRTREIAFVKV 303
             ++Y+T+D        E  +G +   ++     ++ ++ V
Sbjct: 1270 -LNYATID--------ESDIGTILHILEHKNREQVLYLNV 1293

BLAST of CmaCh00G000070 vs. ExPASy Swiss-Prot
Match: P0CT34 (Transposon Tf2-1 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-1 PE=3 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.6e-25
Identity = 79/280 (28.21%), Postives = 135/280 (48.21%), Query Frame = 0

Query: 26   AQLYMKEIVRLHGVPVSIVSYRDPRFTSAFWRGLQKALGTRLDFSTAYHPQTNGQTECLN 85
            A+++ + ++   G P  I++  D  FTS  W+         + FS  Y PQT+GQTE  N
Sbjct: 1030 ARMFDQRVIAYFGNPKEIIADNDHIFTSQTWKDFAHKYNFVMKFSLPYRPQTDGQTERTN 1089

Query: 86   QVLEDMLRARVLDFSGSWDSILHLMEFTYNNSYQATIGMAPYEALYR-RRCRTPLCWGEV 145
            Q +E +LR        +W   + L++ +YNN+  +   M P+E ++R     +PL     
Sbjct: 1090 QTVEKLLRCVCSTHPNTWVDHISLVQQSYNNAIHSATQMTPFEIVHRYSPALSPLELPSF 1149

Query: 146  GERELVGPELVQVTNEAMQQIKGRMRTAQSRQKSYADSRRRNL-EFEVGDMVFLKVAPMK 205
             ++     E  Q T +  Q +K  + T   + K Y D + + + EF+ GD+V +K     
Sbjct: 1150 SDKT---DENSQETIQVFQTVKEHLNTNNIKMKKYFDMKIQEIEEFQPGDLVMVK-RTKT 1209

Query: 206  GVLRFGRKGKLSPRFIGPFGILERIGPIEYRLALSPPVSSI-HNVFHVSTLRKYLANPTH 265
            G L   +  KL+P F GPF +L++ GP  Y L L   +  +  + FHVS L KY  N   
Sbjct: 1210 GFLH--KSNKLAPSFAGPFYVLQKSGPNNYELDLPDSIKHMFSSTFHVSHLEKYRHNSE- 1269

Query: 266  VIDYSTLDMTKDLSYEEKPVGVLARKVKTLRTREIAFVKV 303
             ++Y+T+D        E  +G +   ++     ++ ++ V
Sbjct: 1270 -LNYATID--------ESDIGTILHILEHKNREQVLYLNV 1293

BLAST of CmaCh00G000070 vs. ExPASy Swiss-Prot
Match: P0CT35 (Transposon Tf2-2 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-2 PE=3 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.6e-25
Identity = 79/280 (28.21%), Postives = 135/280 (48.21%), Query Frame = 0

Query: 26   AQLYMKEIVRLHGVPVSIVSYRDPRFTSAFWRGLQKALGTRLDFSTAYHPQTNGQTECLN 85
            A+++ + ++   G P  I++  D  FTS  W+         + FS  Y PQT+GQTE  N
Sbjct: 1030 ARMFDQRVIAYFGNPKEIIADNDHIFTSQTWKDFAHKYNFVMKFSLPYRPQTDGQTERTN 1089

Query: 86   QVLEDMLRARVLDFSGSWDSILHLMEFTYNNSYQATIGMAPYEALYR-RRCRTPLCWGEV 145
            Q +E +LR        +W   + L++ +YNN+  +   M P+E ++R     +PL     
Sbjct: 1090 QTVEKLLRCVCSTHPNTWVDHISLVQQSYNNAIHSATQMTPFEIVHRYSPALSPLELPSF 1149

Query: 146  GERELVGPELVQVTNEAMQQIKGRMRTAQSRQKSYADSRRRNL-EFEVGDMVFLKVAPMK 205
             ++     E  Q T +  Q +K  + T   + K Y D + + + EF+ GD+V +K     
Sbjct: 1150 SDKT---DENSQETIQVFQTVKEHLNTNNIKMKKYFDMKIQEIEEFQPGDLVMVK-RTKT 1209

Query: 206  GVLRFGRKGKLSPRFIGPFGILERIGPIEYRLALSPPVSSI-HNVFHVSTLRKYLANPTH 265
            G L   +  KL+P F GPF +L++ GP  Y L L   +  +  + FHVS L KY  N   
Sbjct: 1210 GFLH--KSNKLAPSFAGPFYVLQKSGPNNYELDLPDSIKHMFSSTFHVSHLEKYRHNSE- 1269

Query: 266  VIDYSTLDMTKDLSYEEKPVGVLARKVKTLRTREIAFVKV 303
             ++Y+T+D        E  +G +   ++     ++ ++ V
Sbjct: 1270 -LNYATID--------ESDIGTILHILEHKNREQVLYLNV 1293

BLAST of CmaCh00G000070 vs. ExPASy Swiss-Prot
Match: P0CT36 (Transposon Tf2-3 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-3 PE=1 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.6e-25
Identity = 79/280 (28.21%), Postives = 135/280 (48.21%), Query Frame = 0

Query: 26   AQLYMKEIVRLHGVPVSIVSYRDPRFTSAFWRGLQKALGTRLDFSTAYHPQTNGQTECLN 85
            A+++ + ++   G P  I++  D  FTS  W+         + FS  Y PQT+GQTE  N
Sbjct: 1030 ARMFDQRVIAYFGNPKEIIADNDHIFTSQTWKDFAHKYNFVMKFSLPYRPQTDGQTERTN 1089

Query: 86   QVLEDMLRARVLDFSGSWDSILHLMEFTYNNSYQATIGMAPYEALYR-RRCRTPLCWGEV 145
            Q +E +LR        +W   + L++ +YNN+  +   M P+E ++R     +PL     
Sbjct: 1090 QTVEKLLRCVCSTHPNTWVDHISLVQQSYNNAIHSATQMTPFEIVHRYSPALSPLELPSF 1149

Query: 146  GERELVGPELVQVTNEAMQQIKGRMRTAQSRQKSYADSRRRNL-EFEVGDMVFLKVAPMK 205
             ++     E  Q T +  Q +K  + T   + K Y D + + + EF+ GD+V +K     
Sbjct: 1150 SDKT---DENSQETIQVFQTVKEHLNTNNIKMKKYFDMKIQEIEEFQPGDLVMVK-RTKT 1209

Query: 206  GVLRFGRKGKLSPRFIGPFGILERIGPIEYRLALSPPVSSI-HNVFHVSTLRKYLANPTH 265
            G L   +  KL+P F GPF +L++ GP  Y L L   +  +  + FHVS L KY  N   
Sbjct: 1210 GFLH--KSNKLAPSFAGPFYVLQKSGPNNYELDLPDSIKHMFSSTFHVSHLEKYRHNSE- 1269

Query: 266  VIDYSTLDMTKDLSYEEKPVGVLARKVKTLRTREIAFVKV 303
             ++Y+T+D        E  +G +   ++     ++ ++ V
Sbjct: 1270 -LNYATID--------ESDIGTILHILEHKNREQVLYLNV 1293

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9UR071.6e-2528.21Transposon Tf2-11 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24... [more]
P0CT411.6e-2528.21Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24... [more]
P0CT341.6e-2528.21Transposon Tf2-1 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 248... [more]
P0CT351.6e-2528.21Transposon Tf2-2 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 248... [more]
P0CT361.6e-2528.21Transposon Tf2-3 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 248... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 19..176
e-value: 1.1E-31
score: 111.6
NoneNo IPR availablePANTHERPTHR46148FAMILY NOT NAMEDcoord: 88..292
NoneNo IPR availablePANTHERPTHR46148:SF18IPP TRANSFERASEcoord: 88..292
IPR001584Integrase, catalytic corePROSITEPS50994INTEGRASEcoord: 24..134
score: 14.201222
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 23..131

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh00G000070.1CmaCh00G000070.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:1901800 positive regulation of proteasomal protein catabolic process
biological_process GO:0006278 RNA-dependent DNA biosynthetic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0000502 proteasome complex
molecular_function GO:0004190 aspartic-type endopeptidase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0004519 endonuclease activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0036402 proteasome-activating activity
molecular_function GO:0003964 RNA-directed DNA polymerase activity
molecular_function GO:0008194 UDP-glycosyltransferase activity
molecular_function GO:0008270 zinc ion binding