CmUC11G204880 (gene) Watermelon (USVL531) v1

Overview
NameCmUC11G204880
Typegene
OrganismCitrullus mucosospermus (Watermelon (USVL531) v1)
DescriptionrRNA N-glycosidase
LocationCmU531Chr11: 1287483 .. 1288304 (+)
RNA-Seq ExpressionCmUC11G204880
SyntenyCmUC11G204880
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATAGATTATCAACGCTTTCACTGCTAGTTCTAGCCATCTTCCTTGGAGGTTCATCCTTCACTGCAGGCGATGTAAGTTTCAGTTTATCCGGTTCGACTTCCTCGTCTTACAGCAAGTTCATCAAAGATCTGAGGAACGCTCTTCCGTCGAAGGCCAAAGTATACAATATAACTCTACTACTCAGCTCCGCCACCGGCGCAAGTCGCTACACACTAATGAAACTCTCCAACTATGACGGTAAAACCATAACAGTTGCTATAGATGTAACAAACGTCTATATCATGGGGTATCTCGTCAACACAACTTCCTACTTTTTCAACGAACCTGATGCTAAATTAGCTTCTCAACATGTATTCAAAGGCAGCAGCATTATTACACTTCCATACTCTGGCAATTACGAGAGGCTTCAAATTGCTGCAGGCAAAAACAGAGAGAGAATTCCTCTTGGATTTCGAGCTTTGGACAGTGCAATTACAAGCTTGTTTCATTATGATTCCACAACTTCTGCTGCAGCATTTCTTGTGATAATTCAGTCCACAGCTGAGGCATCAAGATATAAATATATTGAAGGTCAAATTATTGAGAGAATTCATAAAGATGAGGTTCCAAGTTTGGCTACTATTAGTTTAGAAAATAGTTGGTCTGCTTTATCTAAGCAGATTCAATTGGCACAAACTAATAATGGAAACTTTAAAACTCCTGTTGGGATTATAGATAATCAAGGCAAACGAGTTGAAATAACCAATGTGAATTCGAAAGTTGTGAGTAACAATATCAAGTTGCTGCTAAACTCACAAAATATTGCAATGAGGCCATGA

mRNA sequence

ATGAATAGATTATCAACGCTTTCACTGCTAGTTCTAGCCATCTTCCTTGGAGGTTCATCCTTCACTGCAGGCGATGTAAGTTTCAGTTTATCCGGTTCGACTTCCTCGTCTTACAGCAAGTTCATCAAAGATCTGAGGAACGCTCTTCCGTCGAAGGCCAAAGTATACAATATAACTCTACTACTCAGCTCCGCCACCGGCGCAAGTCGCTACACACTAATGAAACTCTCCAACTATGACGGTAAAACCATAACAGTTGCTATAGATGTAACAAACGTCTATATCATGGGGTATCTCGTCAACACAACTTCCTACTTTTTCAACGAACCTGATGCTAAATTAGCTTCTCAACATGTATTCAAAGGCAGCAGCATTATTACACTTCCATACTCTGGCAATTACGAGAGGCTTCAAATTGCTGCAGGCAAAAACAGAGAGAGAATTCCTCTTGGATTTCGAGCTTTGGACAGTGCAATTACAAGCTTGTTTCATTATGATTCCACAACTTCTGCTGCAGCATTTCTTGTGATAATTCAGTCCACAGCTGAGGCATCAAGATATAAATATATTGAAGGTCAAATTATTGAGAGAATTCATAAAGATGAGGTTCCAAGTTTGGCTACTATTAGTTTAGAAAATAGTTGGTCTGCTTTATCTAAGCAGATTCAATTGGCACAAACTAATAATGGAAACTTTAAAACTCCTGTTGGGATTATAGATAATCAAGGCAAACGAGTTGAAATAACCAATGTGAATTCGAAAGTTGTGAGTAACAATATCAAGTTGCTGCTAAACTCACAAAATATTGCAATGAGGCCATGA

Coding sequence (CDS)

ATGAATAGATTATCAACGCTTTCACTGCTAGTTCTAGCCATCTTCCTTGGAGGTTCATCCTTCACTGCAGGCGATGTAAGTTTCAGTTTATCCGGTTCGACTTCCTCGTCTTACAGCAAGTTCATCAAAGATCTGAGGAACGCTCTTCCGTCGAAGGCCAAAGTATACAATATAACTCTACTACTCAGCTCCGCCACCGGCGCAAGTCGCTACACACTAATGAAACTCTCCAACTATGACGGTAAAACCATAACAGTTGCTATAGATGTAACAAACGTCTATATCATGGGGTATCTCGTCAACACAACTTCCTACTTTTTCAACGAACCTGATGCTAAATTAGCTTCTCAACATGTATTCAAAGGCAGCAGCATTATTACACTTCCATACTCTGGCAATTACGAGAGGCTTCAAATTGCTGCAGGCAAAAACAGAGAGAGAATTCCTCTTGGATTTCGAGCTTTGGACAGTGCAATTACAAGCTTGTTTCATTATGATTCCACAACTTCTGCTGCAGCATTTCTTGTGATAATTCAGTCCACAGCTGAGGCATCAAGATATAAATATATTGAAGGTCAAATTATTGAGAGAATTCATAAAGATGAGGTTCCAAGTTTGGCTACTATTAGTTTAGAAAATAGTTGGTCTGCTTTATCTAAGCAGATTCAATTGGCACAAACTAATAATGGAAACTTTAAAACTCCTGTTGGGATTATAGATAATCAAGGCAAACGAGTTGAAATAACCAATGTGAATTCGAAAGTTGTGAGTAACAATATCAAGTTGCTGCTAAACTCACAAAATATTGCAATGAGGCCATGA

Protein sequence

MNRLSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITLLLSSATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQHVFKGSSIITLPYSGNYERLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQSTAEASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGIIDNQGKRVEITNVNSKVVSNNIKLLLNSQNIAMRP
Homology
BLAST of CmUC11G204880 vs. NCBI nr
Match: Q00465.1 (RecName: Full=Ribosome-inactivating protein luffin-alpha; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Luffa aegyptiaca] >CAA44229.1 a-luffin [Luffa aegyptiaca])

HSP 1 Score: 388.7 bits (997), Expect = 4.3e-104
Identity = 210/268 (78.36%), Postives = 238/268 (88.81%), Query Frame = 0

Query: 4   LSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITLLLS 63
           +   ++L+LAIF+  S+  A DV FSLSGS+S+SYSKFI DLR ALPS   VYNITLLLS
Sbjct: 1   MKRFTVLILAIFVAASTVEA-DVRFSLSGSSSTSYSKFIGDLRKALPSNGTVYNITLLLS 60

Query: 64  SATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQHVFKGS 123
           SA+GASRYTLM LSNYDGK ITVA+DVTNVYIMGYLVN+TSYFFNE DAKLASQ+VFKGS
Sbjct: 61  SASGASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGS 120

Query: 124 SIITLPYSGNYERLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQSTAE 183
           +I+TLPYSGNYE+LQ AAGK RE+IPLGF ALDSAIT+LFHYDST +AAAFLVIIQ+TAE
Sbjct: 121 TIVTLPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAE 180

Query: 184 ASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGIIDNQG 243
           ASR+KYIEGQIIERI K++VPSLATISLEN WSALSKQIQLAQTNNG FKTPV I D++G
Sbjct: 181 ASRFKYIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDKG 240

Query: 244 KRVEITNVNSKVVSNNIKLLLN-SQNIA 271
           +RVEITNV SKVV+ NI+LLLN  QN+A
Sbjct: 241 QRVEITNVTSKVVTKNIQLLLNYKQNVA 267

BLAST of CmUC11G204880 vs. NCBI nr
Match: XP_022953306.1 (ribosome-inactivating protein luffaculin 1-like [Cucurbita moschata])

HSP 1 Score: 384.4 bits (986), Expect = 8.1e-103
Identity = 206/270 (76.30%), Postives = 236/270 (87.41%), Query Frame = 0

Query: 1   MNRLSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITL 60
           MN +S   L++LAIF G SS  AGDVSF+L GS S SYS FI+ LR+ALPSK KVYNI+L
Sbjct: 11  MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 70

Query: 61  LLSSATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQHVF 120
           LL SATGASRYT +KLSNYDGK ITVAIDVTNVYIMGYLVN+TSYFFNE DA+LAS++VF
Sbjct: 71  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 130

Query: 121 KGSSIITLPYSGNYERLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQS 180
           K S+ ITLPYSGNYE+LQ AAGK RE+IPLGF ALD+AIT+LFHYDST +AAAF+V+IQ 
Sbjct: 131 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 190

Query: 181 TAEASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGIID 240
           TAEASRY+YIE Q+I+RI KD+VPSLA ISLENSWSALSKQIQLAQTN G FK PV I D
Sbjct: 191 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 250

Query: 241 NQGKRVEITNVNSKVVSNNIKLLLNSQNIA 271
           +QG+RVEITNV+SKVV+ NI+LLLNS+NIA
Sbjct: 251 DQGQRVEITNVSSKVVAKNIQLLLNSKNIA 280

BLAST of CmUC11G204880 vs. NCBI nr
Match: XP_022992350.1 (ribosome-inactivating protein luffaculin 1-like [Cucurbita maxima])

HSP 1 Score: 384.4 bits (986), Expect = 8.1e-103
Identity = 208/270 (77.04%), Postives = 234/270 (86.67%), Query Frame = 0

Query: 1   MNRLSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITL 60
           MN +S   L++LAIF G SS  AGDVSF+L GS S SYS FIK LR+ALPSK KVYNI+L
Sbjct: 11  MNVVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISL 70

Query: 61  LLSSATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQHVF 120
           LL SATGASRYT +KLSNYDGK ITVAIDVTNVYIMGYLVN+TSYFFNE DA+LAS++VF
Sbjct: 71  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 130

Query: 121 KGSSIITLPYSGNYERLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQS 180
           K S+ ITLPYSGNYE+LQ AAGK RE+IPLGF ALDSAIT+LFHYDST +AAAF+V+IQ 
Sbjct: 131 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQC 190

Query: 181 TAEASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGIID 240
           TAEASRYKYIE Q+I+RI KD+VPS A ISLENSWSALSKQIQLAQTN G FK PV I D
Sbjct: 191 TAEASRYKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 250

Query: 241 NQGKRVEITNVNSKVVSNNIKLLLNSQNIA 271
           +QG+RVEITNV+S VV+ NI+LLLNSQNIA
Sbjct: 251 DQGQRVEITNVSSNVVTKNIQLLLNSQNIA 280

BLAST of CmUC11G204880 vs. NCBI nr
Match: KAG6575376.1 (hypothetical protein SDJN03_26015, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013917.1 hypothetical protein SDJN02_24086, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 384.4 bits (986), Expect = 8.1e-103
Identity = 206/270 (76.30%), Postives = 236/270 (87.41%), Query Frame = 0

Query: 1   MNRLSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITL 60
           MN +S   L++LAIF G SS  AGDVSF+L GS S SYS FI+ LR+ALPSK KVYNI+L
Sbjct: 1   MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 60

Query: 61  LLSSATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQHVF 120
           LL SATGASRYT +KLSNYDGK ITVAIDVTNVYIMGYLVN+TSYFFNE DA+LAS++VF
Sbjct: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120

Query: 121 KGSSIITLPYSGNYERLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQS 180
           K S+ ITLPYSGNYE+LQ AAGK RE+IPLGF ALD+AIT+LFHYDST +AAAF+V+IQ 
Sbjct: 121 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 180

Query: 181 TAEASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGIID 240
           TAEASRY+YIE Q+I+RI KD+VPSLA ISLENSWSALSKQIQLAQTN G FK PV I D
Sbjct: 181 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 240

Query: 241 NQGKRVEITNVNSKVVSNNIKLLLNSQNIA 271
           +QG+RVEITNV+SKVV+ NI+LLLNS+NIA
Sbjct: 241 DQGQRVEITNVSSKVVAKNIQLLLNSKNIA 270

BLAST of CmUC11G204880 vs. NCBI nr
Match: XP_023520860.1 (ribosome-inactivating protein luffaculin 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 383.3 bits (983), Expect = 1.8e-102
Identity = 205/270 (75.93%), Postives = 236/270 (87.41%), Query Frame = 0

Query: 1   MNRLSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITL 60
           MN +S   L++LAIF G SS  AGDVSF+L GS S SYS FI+ LR+ALPSK KVYNI+L
Sbjct: 11  MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 70

Query: 61  LLSSATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQHVF 120
           L+ SATGASRYT +KLSNYDGK ITVAIDVTNVYIMGYLVN+TSYFFNE DA+LAS++VF
Sbjct: 71  LVGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 130

Query: 121 KGSSIITLPYSGNYERLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQS 180
           K S+ ITLPYSGNYE+LQ AAGK RE+IPLGF ALD+AIT+LFHYDST +AAAF+V+IQ 
Sbjct: 131 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFMVVIQC 190

Query: 181 TAEASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGIID 240
           TAEASRY+YIE Q+I+RI KD+VPSLA ISLENSWSALSKQIQLAQTN G FK PV I D
Sbjct: 191 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 250

Query: 241 NQGKRVEITNVNSKVVSNNIKLLLNSQNIA 271
           +QG+RVEITNV+SKVV+ NI+LLLNS+NIA
Sbjct: 251 DQGQRVEITNVSSKVVAKNIQLLLNSKNIA 280

BLAST of CmUC11G204880 vs. ExPASy Swiss-Prot
Match: Q00465 (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 5.6e-107
Identity = 210/268 (78.36%), Postives = 238/268 (88.81%), Query Frame = 0

Query: 4   LSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITLLLS 63
           +   ++L+LAIF+  S+  A DV FSLSGS+S+SYSKFI DLR ALPS   VYNITLLLS
Sbjct: 1   MKRFTVLILAIFVAASTVEA-DVRFSLSGSSSTSYSKFIGDLRKALPSNGTVYNITLLLS 60

Query: 64  SATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQHVFKGS 123
           SA+GASRYTLM LSNYDGK ITVA+DVTNVYIMGYLVN+TSYFFNE DAKLASQ+VFKGS
Sbjct: 61  SASGASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGS 120

Query: 124 SIITLPYSGNYERLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQSTAE 183
           +I+TLPYSGNYE+LQ AAGK RE+IPLGF ALDSAIT+LFHYDST +AAAFLVIIQ+TAE
Sbjct: 121 TIVTLPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAE 180

Query: 184 ASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGIIDNQG 243
           ASR+KYIEGQIIERI K++VPSLATISLEN WSALSKQIQLAQTNNG FKTPV I D++G
Sbjct: 181 ASRFKYIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDKG 240

Query: 244 KRVEITNVNSKVVSNNIKLLLN-SQNIA 271
           +RVEITNV SKVV+ NI+LLLN  QN+A
Sbjct: 241 QRVEITNVTSKVVTKNIQLLLNYKQNVA 267

BLAST of CmUC11G204880 vs. ExPASy Swiss-Prot
Match: P84530 (Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula OX=56866 PE=1 SV=2)

HSP 1 Score: 370.5 bits (950), Expect = 1.6e-101
Identity = 198/241 (82.16%), Postives = 217/241 (90.04%), Query Frame = 0

Query: 25  DVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITLLLSSATGASRYTLMKLSNYDGKTI 84
           DVSFSLSGS+S+SYSKFI  LR ALPS   VYNITLLLSSA+GASRYTLMKLSNYDGK I
Sbjct: 1   DVSFSLSGSSSTSYSKFIGALRKALPSNGTVYNITLLLSSASGASRYTLMKLSNYDGKAI 60

Query: 85  TVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQHVFKGSSIITLPYSGNYERLQIAAGKN 144
           TVAIDVTNVYIMGYLVN+TSYFFNE DAKLASQ+VF GS+I+TLPYSGNYE+LQ AAGK 
Sbjct: 61  TVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFAGSTIVTLPYSGNYEKLQTAAGKI 120

Query: 145 RERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQSTAEASRYKYIEGQIIERIHKDEVP 204
           RE+IPLGF ALDSAIT+LFHYDST +AAAFLVIIQ+TAE+SR+KYIEGQII RI K+ VP
Sbjct: 121 REKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAESSRFKYIEGQIIMRISKNGVP 180

Query: 205 SLATISLENSWSALSKQIQLAQTNNGNFKTPVGIIDNQGKRVEITNVNSKVVSNNIKLLL 264
           SLATISLEN WSALSKQIQLAQTNNG FKTPV I+D  G+RVEI NV SKVV+ NI+LLL
Sbjct: 181 SLATISLENEWSALSKQIQLAQTNNGTFKTPVVIMDAGGQRVEIGNVGSKVVTKNIQLLL 240

Query: 265 N 266
           N
Sbjct: 241 N 241

BLAST of CmUC11G204880 vs. ExPASy Swiss-Prot
Match: P16094 (Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=2)

HSP 1 Score: 360.5 bits (924), Expect = 1.6e-98
Identity = 194/271 (71.59%), Postives = 227/271 (83.76%), Query Frame = 0

Query: 1   MNRLSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITL 60
           M+R S LS L+LAIFLGG S   GDVSF LSG+   SY  FIKDLRNALP + KVYNI L
Sbjct: 1   MSRFSVLSFLILAIFLGG-SIVKGDVSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPL 60

Query: 61  LLSSATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQHVF 120
           LL S +GA RY LM L NYDGKTITVA+DVTNVYIMGYL +TTSYFFNEP A+LASQ+VF
Sbjct: 61  LLPSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVF 120

Query: 121 KGS-SIITLPYSGNYERLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQ 180
           + +   ITLPYSGNYERLQIAAGK RE+IP+G  ALDSAI++L HYDST +A A LV+IQ
Sbjct: 121 RDARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQ 180

Query: 181 STAEASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGII 240
           +TAEA+R+KYIE QI ER ++DEVPSLATISLENSWS LSKQIQLAQ NNG F+TP+ ++
Sbjct: 181 TTAEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLV 240

Query: 241 DNQGKRVEITNVNSKVVSNNIKLLLNSQNIA 271
           DN+G RV+ITNV SKVV++NI+LLLN++NIA
Sbjct: 241 DNKGNRVQITNVTSKVVTSNIQLLLNTRNIA 270

BLAST of CmUC11G204880 vs. ExPASy Swiss-Prot
Match: P22851 (Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 7.6e-96
Identity = 192/249 (77.11%), Postives = 217/249 (87.15%), Query Frame = 0

Query: 25  DVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITLLLSSATGASRYTLMKLSNYDGKTI 84
           +VSFSLSG+ S SYSKFI  LR ALPSK KV NI LLL SA+GASRY LM+LSNYD K I
Sbjct: 2   NVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKAI 61

Query: 85  TVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQHVFKGSSIITLPYSGNYERLQIAAGKN 144
           T+AIDVTNVYIMGYLVN+TSYF NE DAKLASQ+VFKGS+++T+PYSGNYERLQ AAGK 
Sbjct: 62  TMAIDVTNVYIMGYLVNSTSYFANESDAKLASQYVFKGSTLVTIPYSGNYERLQNAAGKI 121

Query: 145 RERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQSTAEASRYKYIEGQIIERIHKDEVP 204
           RE+IPLGFRALDSA+TS+FHYDST +AAAFLVI+Q+TAEASR+KYIEGQIIERI K+EVP
Sbjct: 122 REKIPLGFRALDSALTSIFHYDSTAAAAAFLVILQTTAEASRFKYIEGQIIERIPKNEVP 181

Query: 205 SLATISLEN-SWSALSKQIQLAQTNNGNFKTPVGIIDNQGKRVEITNVNSKVVSN--NIK 264
           S A +SLEN +WS LSKQIQLAQTNNG F+TPV IIDN+G+RVEITN+ SKV     N K
Sbjct: 182 SPAALSLENEAWSLLSKQIQLAQTNNGAFRTPVVIIDNKGQRVEITNLASKVQIKDVNSK 241

Query: 265 LLLNSQNIA 271
           LLLN QNIA
Sbjct: 242 LLLNKQNIA 250

BLAST of CmUC11G204880 vs. ExPASy Swiss-Prot
Match: Q9FRX4 (Putative ribosome-inactivating protein OS=Cucumis ficifolius OX=131071 PE=2 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 3.9e-84
Identity = 169/272 (62.13%), Postives = 209/272 (76.84%), Query Frame = 0

Query: 1   MNRLSTLSLLVLAIFLGGSSFTAGDVS--FSLSGSTSSSYSKFIKDLRNALPSKAKVYNI 60
           MNR S L  LV+     G     GDV+  FSL GS   SYSKFI  +RNALP+   +YNI
Sbjct: 1   MNRFSVLMCLVILSIFHGVPTAEGDVTVKFSLLGSNHKSYSKFITSMRNALPNAGDIYNI 60

Query: 61  TLLLSSATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQH 120
            LL+ S +G+ RY LM+LSNY+G TIT+A+DVTNVYIMGYLVN TSYFFNE DA+LAS+ 
Sbjct: 61  PLLVPSISGSRRYILMQLSNYEGNTITMAVDVTNVYIMGYLVNGTSYFFNETDAQLASKF 120

Query: 121 VFKGSSIITLPYSGNYERLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVII 180
           VF+G+  ITLPYSGNY++LQ  A K R+ IPLGF ALDSAI++L++YDS ++  AFLV+I
Sbjct: 121 VFQGTKSITLPYSGNYQKLQSVARKERDSIPLGFMALDSAISTLYYYDSRSAPIAFLVLI 180

Query: 181 QSTAEASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGI 240
           Q+TAEA+RYKYIE QII+RI   +VP LA ISLEN WS LSKQIQ+A++NNG F+TPV I
Sbjct: 181 QTTAEAARYKYIEKQIIDRISVSKVPDLAAISLENEWSLLSKQIQIAKSNNGQFQTPVKI 240

Query: 241 IDNQGKRVEITNVNSKVVSNNIKLLLNSQNIA 271
           I+++G   E+TNV+S VV+ NI LLLN  NIA
Sbjct: 241 INDKGILTEVTNVSSLVVTKNIMLLLNKLNIA 272

BLAST of CmUC11G204880 vs. ExPASy TrEMBL
Match: A0A6J1JVG7 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111488687 PE=3 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 3.9e-103
Identity = 208/270 (77.04%), Postives = 234/270 (86.67%), Query Frame = 0

Query: 1   MNRLSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITL 60
           MN +S   L++LAIF G SS  AGDVSF+L GS S SYS FIK LR+ALPSK KVYNI+L
Sbjct: 11  MNVVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISL 70

Query: 61  LLSSATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQHVF 120
           LL SATGASRYT +KLSNYDGK ITVAIDVTNVYIMGYLVN+TSYFFNE DA+LAS++VF
Sbjct: 71  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 130

Query: 121 KGSSIITLPYSGNYERLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQS 180
           K S+ ITLPYSGNYE+LQ AAGK RE+IPLGF ALDSAIT+LFHYDST +AAAF+V+IQ 
Sbjct: 131 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQC 190

Query: 181 TAEASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGIID 240
           TAEASRYKYIE Q+I+RI KD+VPS A ISLENSWSALSKQIQLAQTN G FK PV I D
Sbjct: 191 TAEASRYKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 250

Query: 241 NQGKRVEITNVNSKVVSNNIKLLLNSQNIA 271
           +QG+RVEITNV+S VV+ NI+LLLNSQNIA
Sbjct: 251 DQGQRVEITNVSSNVVTKNIQLLLNSQNIA 280

BLAST of CmUC11G204880 vs. ExPASy TrEMBL
Match: A0A6J1GMM2 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455894 PE=3 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 3.9e-103
Identity = 206/270 (76.30%), Postives = 236/270 (87.41%), Query Frame = 0

Query: 1   MNRLSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITL 60
           MN +S   L++LAIF G SS  AGDVSF+L GS S SYS FI+ LR+ALPSK KVYNI+L
Sbjct: 11  MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 70

Query: 61  LLSSATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQHVF 120
           LL SATGASRYT +KLSNYDGK ITVAIDVTNVYIMGYLVN+TSYFFNE DA+LAS++VF
Sbjct: 71  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 130

Query: 121 KGSSIITLPYSGNYERLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQS 180
           K S+ ITLPYSGNYE+LQ AAGK RE+IPLGF ALD+AIT+LFHYDST +AAAF+V+IQ 
Sbjct: 131 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 190

Query: 181 TAEASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGIID 240
           TAEASRY+YIE Q+I+RI KD+VPSLA ISLENSWSALSKQIQLAQTN G FK PV I D
Sbjct: 191 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 250

Query: 241 NQGKRVEITNVNSKVVSNNIKLLLNSQNIA 271
           +QG+RVEITNV+SKVV+ NI+LLLNS+NIA
Sbjct: 251 DQGQRVEITNVSSKVVAKNIQLLLNSKNIA 280

BLAST of CmUC11G204880 vs. ExPASy TrEMBL
Match: B0EVM6 (rRNA N-glycosidase OS=Luffa acutangula OX=56866 GN=RIPI PE=2 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 7.4e-102
Identity = 208/270 (77.04%), Postives = 232/270 (85.93%), Query Frame = 0

Query: 1   MNRLSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITL 60
           MNR + LSLL+  I +   +    +VSFSLSG+ S SYSKFI  LR ALPSK KV NI L
Sbjct: 1   MNRFTFLSLLI--ILIAFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPL 60

Query: 61  LLSSATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQHVF 120
           LL SA+GASRY LM+LSNYD K IT+AIDVTNVYIMGYLVN+TSYFFNE DAKLASQ+VF
Sbjct: 61  LLPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF 120

Query: 121 KGSSIITLPYSGNYERLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQS 180
           KGS+I+TLPYSGNYERLQ AAGK RE+IPLGFRA DSAITSLFHYDST +A AFLVIIQ+
Sbjct: 121 KGSTIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQT 180

Query: 181 TAEASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGIID 240
           TAEASR+KYIEGQIIERI K+EVPS A +SLEN WSALSKQIQLAQTNNG F+TPV IID
Sbjct: 181 TAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIID 240

Query: 241 NQGKRVEITNVNSKVVSNNIKLLLNSQNIA 271
           N+G+RVEI +VNSKVV+NNIKLLLN QNIA
Sbjct: 241 NKGQRVEIKDVNSKVVTNNIKLLLNKQNIA 268

BLAST of CmUC11G204880 vs. ExPASy TrEMBL
Match: Q00980 (rRNA N-glycosidase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 7.4e-102
Identity = 208/270 (77.04%), Postives = 232/270 (85.93%), Query Frame = 0

Query: 1   MNRLSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITL 60
           MNR + LSLL+  I +   +    +VSFSLSG+ S SYSKFI  LR ALPSK KV NI L
Sbjct: 1   MNRFTFLSLLI--ILIAFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPL 60

Query: 61  LLSSATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQHVF 120
           LL SA+GASRY LM+LSNYD K IT+AIDVTNVYIMGYLVN+TSYFFNE DAKLASQ+VF
Sbjct: 61  LLPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF 120

Query: 121 KGSSIITLPYSGNYERLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQS 180
           KGS+I+TLPYSGNYERLQ AAGK RE+IPLGFRA DSAITSLFHYDST +A AFLVIIQ+
Sbjct: 121 KGSTIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQT 180

Query: 181 TAEASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGIID 240
           TAEASR+KYIEGQIIERI K+EVPS A +SLEN WSALSKQIQLAQTNNG F+TPV IID
Sbjct: 181 TAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIID 240

Query: 241 NQGKRVEITNVNSKVVSNNIKLLLNSQNIA 271
           N+G+RVEI +VNSKVV+NNIKLLLN QNIA
Sbjct: 241 NKGQRVEIKDVNSKVVTNNIKLLLNKQNIA 268

BLAST of CmUC11G204880 vs. ExPASy TrEMBL
Match: A0A6J1DSN8 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023551 PE=3 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 6.0e-96
Identity = 194/271 (71.59%), Postives = 227/271 (83.76%), Query Frame = 0

Query: 1   MNRLSTLSLLVLAIFLGGSSFTAGDVSFSLSGSTSSSYSKFIKDLRNALPSKAKVYNITL 60
           M+R S LS L+LAIFLGG S   GDVSF LSG+   SY  FIKDLRNALP + KVYNI L
Sbjct: 1   MSRFSVLSFLILAIFLGG-SIVKGDVSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPL 60

Query: 61  LLSSATGASRYTLMKLSNYDGKTITVAIDVTNVYIMGYLVNTTSYFFNEPDAKLASQHVF 120
           LL S +GA RY LM L NYDGKTITVA+DVTNVYIMGYL +TTSYFFNEP A+LASQ+VF
Sbjct: 61  LLPSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVF 120

Query: 121 KGS-SIITLPYSGNYERLQIAAGKNRERIPLGFRALDSAITSLFHYDSTTSAAAFLVIIQ 180
           + +   ITLPYSGNYERLQIAAGK RE+IP+G  ALDSAI++L HYDST +A A LV+IQ
Sbjct: 121 RDARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQ 180

Query: 181 STAEASRYKYIEGQIIERIHKDEVPSLATISLENSWSALSKQIQLAQTNNGNFKTPVGII 240
           +TAEA+R+KYIE QI ER ++DEVPSLATISLENSWS LSKQIQLAQ NNG F+TP+ ++
Sbjct: 181 TTAEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLV 240

Query: 241 DNQGKRVEITNVNSKVVSNNIKLLLNSQNIA 271
           DN+G RV+ITNV SKVV++NI+LLLN++NIA
Sbjct: 241 DNKGNRVQITNVTSKVVTSNIQLLLNTRNIA 270

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q00465.14.3e-10478.36RecName: Full=Ribosome-inactivating protein luffin-alpha; AltName: Full=rRNA N-g... [more]
XP_022953306.18.1e-10376.30ribosome-inactivating protein luffaculin 1-like [Cucurbita moschata][more]
XP_022992350.18.1e-10377.04ribosome-inactivating protein luffaculin 1-like [Cucurbita maxima][more]
KAG6575376.18.1e-10376.30hypothetical protein SDJN03_26015, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023520860.11.8e-10275.93ribosome-inactivating protein luffaculin 1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q004655.6e-10778.36Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1[more]
P845301.6e-10182.16Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula OX=56866 PE=1 SV=... [more]
P160941.6e-9871.59Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=... [more]
P228517.6e-9677.11Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca OX=3670 PE=1 SV=1[more]
Q9FRX43.9e-8462.13Putative ribosome-inactivating protein OS=Cucumis ficifolius OX=131071 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JVG73.9e-10377.04rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111488687 PE=3 SV=1[more]
A0A6J1GMM23.9e-10376.30rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455894 PE=3 SV=1[more]
B0EVM67.4e-10277.04rRNA N-glycosidase OS=Luffa acutangula OX=56866 GN=RIPI PE=2 SV=1[more]
Q009807.4e-10277.04rRNA N-glycosidase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1[more]
A0A6J1DSN86.0e-9671.59rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023551 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017989Ribosome-inactivating protein type 1/2PRINTSPR00396SHIGARICINcoord: 36..49
score: 46.05
coord: 83..98
score: 55.43
coord: 145..159
score: 54.91
coord: 174..194
score: 55.26
coord: 206..223
score: 53.22
IPR001574Ribosome-inactivating proteinPFAMPF00161RIPcoord: 34..223
e-value: 1.6E-30
score: 106.2
IPR001574Ribosome-inactivating proteinPANTHERPTHR33453FAMILY NOT NAMEDcoord: 26..268
IPR016139Ribosome-inactivating protein, subdomain 2GENE3D4.10.470.10Ricin (A Subunit), domain 2coord: 186..270
e-value: 9.0E-28
score: 98.0
IPR016138Ribosome-inactivating protein, subdomain 1GENE3D3.40.420.10Ricin (A subunit), domain 1coord: 24..185
e-value: 3.4E-46
score: 158.8
NoneNo IPR availablePANTHERPTHR33453:SF14RRNA N-GLYCOSIDASEcoord: 26..268
IPR017988Ribosome-inactivating protein conserved sitePROSITEPS00275SHIGA_RICINcoord: 178..194
IPR036041Ribosome-inactivating protein superfamilySUPERFAMILY56371Ribosome inactivating proteins (RIP)coord: 25..268

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmUC11G204880.1CmUC11G204880.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051607 defense response to virus
biological_process GO:0035821 modulation of process of other organism
biological_process GO:0017148 negative regulation of translation
molecular_function GO:0030598 rRNA N-glycosylase activity
molecular_function GO:0090729 toxin activity