CmUC11G204140 (gene) Watermelon (USVL531) v1

Overview
NameCmUC11G204140
Typegene
OrganismCitrullus mucosospermus (Watermelon (USVL531) v1)
DescriptionCytochrome p450 76c2
LocationCmU531Chr11: 470108 .. 472246 (+)
RNA-Seq ExpressionCmUC11G204140
SyntenyCmUC11G204140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGTTATTGTGTGTAATGGAATCATGAACTAGAAAAATATGGCATCTCAGGAAGAGTTTCTTGTTTCAGTACTAATATTTCTCCTCCTACCTTTGTTTTTCATTGTTTTCAAATTGTTTAGGTCTCCATGTTCAAAACAACCTCCAATTCCACCTGGCCCAAAACCATGGCCAATTCTTGGAAACCTCCTTCAAATAGGCAGAAATGCTCACATTTCAATCACCCACCTTGCCCAGACCTATGGCCCTCTAATCTCGCTTCGGTTCGGAGGTCGACTACTCGTCGTTGCTTCGTCTCCAGCCGCTGCCGCTGCCGTACTCAAGACCCAAGATCGCCTTCTATCAGGTCGCTACGTTTTTCATATGACTCCTGATCGTGCACAGCATCGCGAATGCTCCCTCGTGTTTTCCCCGGAGTGCAATGATAGTTGGAGGAGTTTAAGGTCCATTTGCAAGGCTAATTTGTTCACAGCAAGAGCAATAGAATCACAGGGTAGTTTGAGGAGGAGGAAAATGAATGAAATGATGGAGTTCTTGTTGTCAAAACAAGGGTCAACAGTGGAGATTAGAGATTTTGTGTTGACTACTGTTTTTAACATTCTCTCTAACTTCATCTTCTCAAGGGATTTGTTTGATTTTGATGGTGATGGATTCAATGGTATCAAACAACCTTTTAGCAATCTTAAGAATTTGGGGTTGACTCCAAATTTAGGCGACTTCTACCCCTTCTTGACAAATTTAGATATTCAAGGTCTTCGAAATAAATGTTCCCTCTACAGTAAGCAAATCAATTCTACTTTTGATATTCTAATCAATGAAAGAAGAAAAGTTTCTTCTCCAAATTCTTCATCGCCACAAGACTTCTTGGATGTGTTGATTCAGAGTCAATTCACTGATGACCAAATTAGCTACTTGGTTATTGTAAGCAAACAAATAAACACCTTTTTTTTTTTTTTTCTTTTTCTTTATTTTCATGTCGAGTAATAATAGGGGCGTTGATCGGTCAGTTGGTGTTGGTTTTAGCCTAAAACGGTTGCCAACCAACCGACCACTTACATGAAATGCTCTCAAATCATCATTTATATTGCACGAATTTCAAATTTTTTTTAAGAAAAAAAAACTTTACATTTCTCTCTCTAACATAGTCGGTCAGTCGATTTCTTCAAATATTTTCAAAACCAACCCTGAGCTACTCCACCTTTAAACTGATCAACCAACACCAACTTGGCAAGTTTCTTTGAACACCCTTGTATTGCTAAGCAACACTTTGGTGCTTCTCAGGCTGCACTCATCTTTATGCAACTCTTCAGACTACTACCAAATGAACGATAAAAAGATGGATGCAGCTCAACTCAAGATGAATTCGTGCTAATCTTTTTATATCTTTTTTGCAAAGTGTAGGAACTTTTGTTGGCAGGCACAGACACAAGTACAATGACAGTGGAGTGGGCAATGGCGGAGGTGTTAAAGAACAAAGACATAATGGAGAAGGTGAGAGAGGAGATGAAAAGAGAAACAAAGCAAAATAGTATTGATGAAACAGAGGTATGTAAGCTTAAGTATCTGTGGAAATGTGTGAAAGAGACTCTAAGATTACACCCACCTGCCCCATTTCTTCTTCCACGCATAGCACCAATGGATTGTGAACTTATGGGATACTCAATTCCAAAGGACACTCTCATTTTTGTCAACATTTGGGGCATTGGAAGAGACCCTTCAATCTGGGAAGATTGTTTATCATTCAATCCTCAGAGATTTAGTGATTATGATCATGTGGAGTTTAAAGGGAATGATTATGGATACCTTCCATTTGGTGGAGGAAGAAGAATTTGCCCTGGCTTTCCAATGGCAACTCTTCAACTTCCTTTAATTTTAGCCACTTTAATTCATAATTTTGAATGGTCACTTCCTGATGGTGAGGATTTTTCAAAGGTGGATATGGAAGGGATGCTTGGTGTAACTTTGCAGAAGAAACAACCACTCAAACTCATTCCTAAAAAGAGGTTTTGAAATATCCAAGTCCGACATCCTCAATTTATCTTTATCAACTATGTTGTATTTGTGTTGTTTAACAATCTGTTACTTGTGTGCTTTTTAATGATTTTTTCAGTATAAGGTAATATATATACATATATAACTT

mRNA sequence

CTGTTATTGTGTGTAATGGAATCATGAACTAGAAAAATATGGCATCTCAGGAAGAGTTTCTTGTTTCAGTACTAATATTTCTCCTCCTACCTTTGTTTTTCATTGTTTTCAAATTGTTTAGGTCTCCATGTTCAAAACAACCTCCAATTCCACCTGGCCCAAAACCATGGCCAATTCTTGGAAACCTCCTTCAAATAGGCAGAAATGCTCACATTTCAATCACCCACCTTGCCCAGACCTATGGCCCTCTAATCTCGCTTCGGTTCGGAGGTCGACTACTCGTCGTTGCTTCGTCTCCAGCCGCTGCCGCTGCCGTACTCAAGACCCAAGATCGCCTTCTATCAGGTCGCTACGTTTTTCATATGACTCCTGATCGTGCACAGCATCGCGAATGCTCCCTCGTGTTTTCCCCGGAGTGCAATGATAGTTGGAGGAGTTTAAGGTCCATTTGCAAGGCTAATTTGTTCACAGCAAGAGCAATAGAATCACAGGGTAGTTTGAGGAGGAGGAAAATGAATGAAATGATGGAGTTCTTGTTGTCAAAACAAGGGTCAACAGTGGAGATTAGAGATTTTGTGTTGACTACTGTTTTTAACATTCTCTCTAACTTCATCTTCTCAAGGGATTTGTTTGATTTTGATGGTGATGGATTCAATGGTATCAAACAACCTTTTAGCAATCTTAAGAATTTGGGGTTGACTCCAAATTTAGGCGACTTCTACCCCTTCTTGACAAATTTAGATATTCAAGGTCTTCGAAATAAATGTTCCCTCTACAGTAAGCAAATCAATTCTACTTTTGATATTCTAATCAATGAAAGAAGAAAAGTTTCTTCTCCAAATTCTTCATCGCCACAAGACTTCTTGGATGTGTTGATTCAGAGTCAATTCACTGATGACCAAATTAGCTACTTGGTTATTGAACTTTTGTTGGCAGGCACAGACACAAGTACAATGACAGTGGAGTGGGCAATGGCGGAGGTGTTAAAGAACAAAGACATAATGGAGAAGGTGAGAGAGGAGATGAAAAGAGAAACAAAGCAAAATAGTATTGATGAAACAGAGGTATGTAAGCTTAAGTATCTGTGGAAATGTGTGAAAGAGACTCTAAGATTACACCCACCTGCCCCATTTCTTCTTCCACGCATAGCACCAATGGATTGTGAACTTATGGGATACTCAATTCCAAAGGACACTCTCATTTTTGTCAACATTTGGGGCATTGGAAGAGACCCTTCAATCTGGGAAGATTGTTTATCATTCAATCCTCAGAGATTTAGTGATTATGATCATGTGGAGTTTAAAGGGAATGATTATGGATACCTTCCATTTGGTGGAGGAAGAAGAATTTGCCCTGGCTTTCCAATGGCAACTCTTCAACTTCCTTTAATTTTAGCCACTTTAATTCATAATTTTGAATGGTCACTTCCTGATGGTGAGGATTTTTCAAAGGTGGATATGGAAGGGATGCTTGGTGTAACTTTGCAGAAGAAACAACCACTCAAACTCATTCCTAAAAAGAGGTTTTGAAATATCCAAGTCCGACATCCTCAATTTATCTTTATCAACTATGTTGTATTTGTGTTGTTTAACAATCTGTTACTTGTGTGCTTTTTAATGATTTTTTCAGTATAAGGTAATATATATACATATATAACTT

Coding sequence (CDS)

ATGGCATCTCAGGAAGAGTTTCTTGTTTCAGTACTAATATTTCTCCTCCTACCTTTGTTTTTCATTGTTTTCAAATTGTTTAGGTCTCCATGTTCAAAACAACCTCCAATTCCACCTGGCCCAAAACCATGGCCAATTCTTGGAAACCTCCTTCAAATAGGCAGAAATGCTCACATTTCAATCACCCACCTTGCCCAGACCTATGGCCCTCTAATCTCGCTTCGGTTCGGAGGTCGACTACTCGTCGTTGCTTCGTCTCCAGCCGCTGCCGCTGCCGTACTCAAGACCCAAGATCGCCTTCTATCAGGTCGCTACGTTTTTCATATGACTCCTGATCGTGCACAGCATCGCGAATGCTCCCTCGTGTTTTCCCCGGAGTGCAATGATAGTTGGAGGAGTTTAAGGTCCATTTGCAAGGCTAATTTGTTCACAGCAAGAGCAATAGAATCACAGGGTAGTTTGAGGAGGAGGAAAATGAATGAAATGATGGAGTTCTTGTTGTCAAAACAAGGGTCAACAGTGGAGATTAGAGATTTTGTGTTGACTACTGTTTTTAACATTCTCTCTAACTTCATCTTCTCAAGGGATTTGTTTGATTTTGATGGTGATGGATTCAATGGTATCAAACAACCTTTTAGCAATCTTAAGAATTTGGGGTTGACTCCAAATTTAGGCGACTTCTACCCCTTCTTGACAAATTTAGATATTCAAGGTCTTCGAAATAAATGTTCCCTCTACAGTAAGCAAATCAATTCTACTTTTGATATTCTAATCAATGAAAGAAGAAAAGTTTCTTCTCCAAATTCTTCATCGCCACAAGACTTCTTGGATGTGTTGATTCAGAGTCAATTCACTGATGACCAAATTAGCTACTTGGTTATTGAACTTTTGTTGGCAGGCACAGACACAAGTACAATGACAGTGGAGTGGGCAATGGCGGAGGTGTTAAAGAACAAAGACATAATGGAGAAGGTGAGAGAGGAGATGAAAAGAGAAACAAAGCAAAATAGTATTGATGAAACAGAGGTATGTAAGCTTAAGTATCTGTGGAAATGTGTGAAAGAGACTCTAAGATTACACCCACCTGCCCCATTTCTTCTTCCACGCATAGCACCAATGGATTGTGAACTTATGGGATACTCAATTCCAAAGGACACTCTCATTTTTGTCAACATTTGGGGCATTGGAAGAGACCCTTCAATCTGGGAAGATTGTTTATCATTCAATCCTCAGAGATTTAGTGATTATGATCATGTGGAGTTTAAAGGGAATGATTATGGATACCTTCCATTTGGTGGAGGAAGAAGAATTTGCCCTGGCTTTCCAATGGCAACTCTTCAACTTCCTTTAATTTTAGCCACTTTAATTCATAATTTTGAATGGTCACTTCCTGATGGTGAGGATTTTTCAAAGGTGGATATGGAAGGGATGCTTGGTGTAACTTTGCAGAAGAAACAACCACTCAAACTCATTCCTAAAAAGAGGTTTTGA

Protein sequence

MASQEEFLVSVLIFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQTYGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDSWRSLRSICKANLFTARAIESQGSLRRRKMNEMMEFLLSKQGSTVEIRDFVLTTVFNILSNFIFSRDLFDFDGDGFNGIKQPFSNLKNLGLTPNLGDFYPFLTNLDIQGLRNKCSLYSKQINSTFDILINERRKVSSPNSSSPQDFLDVLIQSQFTDDQISYLVIELLLAGTDTSTMTVEWAMAEVLKNKDIMEKVREEMKRETKQNSIDETEVCKLKYLWKCVKETLRLHPPAPFLLPRIAPMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFSDYDHVEFKGNDYGYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQKKQPLKLIPKKRF
Homology
BLAST of CmUC11G204140 vs. NCBI nr
Match: XP_038892964.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Benincasa hispida])

HSP 1 Score: 850.9 bits (2197), Expect = 5.5e-243
Identity = 416/497 (83.70%), Postives = 459/497 (92.35%), Query Frame = 0

Query: 1   MASQEEFLVSVLIFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHIS 60
           +ASQE+F++ V+  LLLPL F+VFKL   P SK+PPIPPGPKPWPILGNLLQIG+NAHIS
Sbjct: 9   VASQEDFVLCVI--LLLPLLFVVFKLISFPSSKKPPIPPGPKPWPILGNLLQIGKNAHIS 68

Query: 61  ITHLAQTYGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECS 120
           ITH AQTYGPLISLR GGRLLVVASSPAAAAAVLKTQDRLLS RYVFHMTPD AQH ECS
Sbjct: 69  ITHFAQTYGPLISLRLGGRLLVVASSPAAAAAVLKTQDRLLSNRYVFHMTPDLAQHHECS 128

Query: 121 LVFSPECNDSWRSLRSICKANLFTARAIESQGSLRRRKMNEMMEFLLSKQGSTVEIRDFV 180
           LVFSPECND WRSLRSICKANLFTA+AI+SQ SLRRRKMNEM+EFLLSKQGS VEIRDFV
Sbjct: 129 LVFSPECNDHWRSLRSICKANLFTAKAIKSQASLRRRKMNEMIEFLLSKQGSVVEIRDFV 188

Query: 181 LTTVFNILSNFIFSRDLFDFDGDGFNGIKQPFSNLKNLGLTPNLGDFYPFLTNLDIQGLR 240
           LTTVFN LSNFIFSRDL DFDG+GFNG+KQ F+NLKNLGL PNLGDFYPFL NLD+QGLR
Sbjct: 189 LTTVFNALSNFIFSRDLIDFDGEGFNGLKQSFNNLKNLGLAPNLGDFYPFLRNLDVQGLR 248

Query: 241 NKCSLYSKQINSTFDILINERRKVSSPNSSSPQDFLDVLIQSQFTDDQISYLVIELLLAG 300
           NKCS+YSKQINST DI+IN+RRK++S + S+PQDFLDV+IQSQFT+D I+YLV+ELLLAG
Sbjct: 249 NKCSIYSKQINSTLDIIINQRRKLASTDPSAPQDFLDVMIQSQFTNDHINYLVVELLLAG 308

Query: 301 TDTSTMTVEWAMAEVLKNKDIMEKVREEMKRETKQNSIDETEVCKLKYLWKCVKETLRLH 360
           TDTST+TVEW MAE+LKNK+I+EKVREE+  E KQNSIDE+E+ KLKYLWKCVKETLRLH
Sbjct: 309 TDTSTITVEWTMAELLKNKNIIEKVREEVNTEMKQNSIDESEIYKLKYLWKCVKETLRLH 368

Query: 361 PPAPFLLPRIAPMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFSDYDHVE 420
           PPAPFLLPRIAP+DCELMGYSIPK++LIFVN+WGIGRDPSIWE+ LSFNP RFSD DHV+
Sbjct: 369 PPAPFLLPRIAPIDCELMGYSIPKESLIFVNVWGIGRDPSIWEESLSFNPMRFSDCDHVD 428

Query: 421 FKGNDYGYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDG-EDFSKVDMEGMLG 480
            KGND+GYLPFGGGRRICPGFPMAT+ LPLILA LIHNFEWSLP+G EDFSK+DMEGMLG
Sbjct: 429 LKGNDFGYLPFGGGRRICPGFPMATVLLPLILANLIHNFEWSLPNGDEDFSKMDMEGMLG 488

Query: 481 VTLQKKQPLKLIPKKRF 497
           VTL KKQPLKLIPKKRF
Sbjct: 489 VTLLKKQPLKLIPKKRF 503

BLAST of CmUC11G204140 vs. NCBI nr
Match: XP_022159319.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Momordica charantia])

HSP 1 Score: 715.7 bits (1846), Expect = 2.8e-202
Identity = 355/497 (71.43%), Postives = 415/497 (83.50%), Query Frame = 0

Query: 2   ASQEEFLVSVLIFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISI 61
           A  +E  + + I LLL   FI  K FRSP SKQ  +PPGPKPWPILGNLLQIG +AH S+
Sbjct: 6   ADDQEQQLVLPILLLLASLFITLKYFRSPSSKQTKLPPGPKPWPILGNLLQIGEDAHASM 65

Query: 62  THLAQTYGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSL 121
           T  AQ YGPLISLR GG+LLVVASSP AAA VLKTQDRLLS RY+F    D A HRECS 
Sbjct: 66  TRFAQAYGPLISLRLGGQLLVVASSPDAAAVVLKTQDRLLSARYIFQTVHDEALHRECSF 125

Query: 122 VFSPECNDSWRSLRSICKANLFTARAIESQGSLRRRKMNEMMEFLLSKQGSTVEIRDFVL 181
           VFSPECND WR +RSIC+ANLFTA+AI+SQ +LRR KM+EM+EFL+SKQGS VEIRDFV 
Sbjct: 126 VFSPECNDRWRKVRSICRANLFTAKAIDSQANLRRMKMDEMVEFLVSKQGSVVEIRDFVF 185

Query: 182 TTVFNILSNFIFSRDLFDFDGDGFNGIKQPFSNLKNLGLTPNLGDFYPFLTNLDIQGLRN 241
           TTVFNILSNFIFSRDLFDF GDGFNGIK     +  LGLTPNL D+Y  L NLD+QGLR 
Sbjct: 186 TTVFNILSNFIFSRDLFDFVGDGFNGIKASLKKMVTLGLTPNLADYYSLLRNLDLQGLRK 245

Query: 242 KCSLYSKQINSTFDILINERRKVSSPNSSS--PQDFLDVLIQSQFTDDQISYLVIELLLA 301
           K ++Y KQI+S +  LI+ERRK+   NS +   +DFLDVLIQSQF D+QI++L++E+ LA
Sbjct: 246 KATIYRKQIDSAWKELIDERRKIHLQNSPNFVARDFLDVLIQSQFNDEQINHLIVEIFLA 305

Query: 302 GTDTSTMTVEWAMAEVLKNKDIMEKVREEMKRE-TKQNSIDETEVCKLKYLWKCVKETLR 361
           GTDT+TMTVEWAMAE+LK +DIM KVREEMKRE   Q+++DE+++ +L YLWKCVKETLR
Sbjct: 306 GTDTTTMTVEWAMAEMLKQRDIMAKVREEMKREINNQSTVDESKISQLHYLWKCVKETLR 365

Query: 362 LHPPAPFLLPRIAPMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFSDYDH 421
           LHPPAPFLLPR+AP DCELMGYSIPKDTLIFVN+WGIGRDPS+WED LSFNP+RF+D D 
Sbjct: 366 LHPPAPFLLPRVAPTDCELMGYSIPKDTLIFVNVWGIGRDPSLWEDSLSFNPERFTDCD- 425

Query: 422 VEFKGNDYGYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGML 481
           V+FKG DY YLPFGGGRRICPG PMA++Q+PLILATLIHNFE SLP+G+DFSK+D++G L
Sbjct: 426 VDFKGYDYRYLPFGGGRRICPGLPMASVQIPLILATLIHNFELSLPNGQDFSKLDLDGKL 485

Query: 482 GVTLQKKQPLKLIPKKR 496
           GVTLQKK+PL+LI +KR
Sbjct: 486 GVTLQKKKPLQLILQKR 501

BLAST of CmUC11G204140 vs. NCBI nr
Match: XP_022928791.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata])

HSP 1 Score: 678.3 bits (1749), Expect = 4.9e-191
Identity = 332/483 (68.74%), Postives = 392/483 (81.16%), Query Frame = 0

Query: 13  IFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQTYGPLI 72
           + LLLPL FI+ +   S  SKQPP+PPGPKPWPILGNLLQIG+NAHIS+T  +  YGPLI
Sbjct: 9   LLLLLPLIFIILRHI-SSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLI 68

Query: 73  SLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDSWR 132
           SL+ G + LVVASSPAAAAA+LKTQDRLLS RY+F MTPDR  H  CSL+FSPEC D W+
Sbjct: 69  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 128

Query: 133 SLRSICKANLFTARAIESQGSLRRRKMNEMMEFLLSKQGSTVEIRDFVLTTVFNILSNFI 192
           +LRSIC+A LFTARAIESQ SLRR+KMNEM+EFL SKQGS VEI++ V  TVFN LSNFI
Sbjct: 129 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 188

Query: 193 FSRDLFDFDGDGFNGIKQPFSNLKNLGLTPNLGDFYPFLTNLDIQGLRNKCSLYSKQINS 252
           FSRDL  F GDGFNGIK PF N+ +LGL PNL DFYP L   D+QGLR K ++Y  Q++S
Sbjct: 189 FSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQVDS 248

Query: 253 TFDILINERRKVSSPNSSSPQDFLDVLIQSQFTDDQISYLVIELLLAGTDTSTMTVEWAM 312
           +++ILI ERR + S   S+  DFLDVLIQS FTD+QISYL++E+L AGTDT+T TVEWAM
Sbjct: 249 SWEILIKERRTIHSQGVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTTTTTVEWAM 308

Query: 313 AEVLKNKDIMEKVREEMKRETKQNSIDETEVCKLKYLWKCVKETLRLHPPAPFLLPRIAP 372
           AE+LKN+DIM +VREE K+     +IDE+++ +L YLWKCVKETLRLHPPAPFLLPR AP
Sbjct: 309 AELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAP 368

Query: 373 MDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFSDYDHVEFKGNDYGYLPFG 432
             CELMGYS+PK T+IFVN+WGIGRDPSIWED  SF P+RF   D  + KG+DY +LPFG
Sbjct: 369 EACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCD-ADLKGHDYRFLPFG 428

Query: 433 GGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQKKQPLKLIP 492
           GGRRICPG PMA +Q+PLILATLIHNFEW LP+ ED S +D+ G LGVTLQK++PLKL+ 
Sbjct: 429 GGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLKLLS 488

Query: 493 KKR 496
           K R
Sbjct: 489 KNR 489

BLAST of CmUC11G204140 vs. NCBI nr
Match: KAA0055170.1 (putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo var. makuwa])

HSP 1 Score: 677.6 bits (1747), Expect = 8.4e-191
Identity = 331/487 (67.97%), Postives = 396/487 (81.31%), Query Frame = 0

Query: 13  IFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQTYGPLI 72
           + LLLP+ FI+ K  +SP SK  P+PPGP PWPILGNLLQIG+NAHISIT  A  YGPLI
Sbjct: 13  LLLLLPI-FIILKHIKSPSSKL-PLPPGPNPWPILGNLLQIGQNAHISITQFANIYGPLI 72

Query: 73  SLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDSWR 132
           SL+ G + L+VASSPAAA AVLKTQDRLLS RY+F MTPDRA H + SLVFSPEC D W+
Sbjct: 73  SLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECGDQWK 132

Query: 133 SLRSICKANLFTARAIESQGSLRRRKMNEMMEFLLSKQGSTVEIRDFVLTTVFNILSNFI 192
           +LRS+CK NLFTA+AIESQ  LR +KM E+++FL SKQGS VE++DFV T+VFN LSN I
Sbjct: 133 NLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVFTSVFNTLSNLI 192

Query: 193 FSRDLFDFDGDGFNGIKQPFSNLKNLGLTPNLGDFYPFLTNLDIQGLRNKCSLYSKQINS 252
           FS DL DF GDGF GIK PF  + +LGLTPNL DFYP L   D+QGL+NK  +Y  +++S
Sbjct: 193 FSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKNEVDS 252

Query: 253 TFDILINERRKVSSPNSSSPQDFLDVLIQSQFTDDQISYLVIELLLAGTDTSTMTVEWAM 312
            + ILI ERR++     S+  DFLDVLIQS F D QI+ L+IELL AGTDT+T TVEWAM
Sbjct: 253 LWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGTDTTTTTVEWAM 312

Query: 313 AEVLKNKDIMEKVREEMKRETKQN-SIDETEVCKLKYLWKCVKETLRLHPPAPFLLPRIA 372
           +E+LK+KDI+ KVREEMK+ TK+N  IDE+ + +L+YLW+CVKETLRLHPPAPFLLPR+A
Sbjct: 313 SEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRLHPPAPFLLPRLA 372

Query: 373 PMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFS---DYDHVEFKGNDYGY 432
           PMDCE+MGYSIPKD+++FVN+WGIGRDPS+WED  +FNPQRF      D+V+FKG DY Y
Sbjct: 373 PMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVDFKGYDYRY 432

Query: 433 LPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQKKQPL 492
           LPFGGGRRICPG PMA +Q+PLILATLIHNFEWSLP+ ED SK+D+ G LGVTLQK + L
Sbjct: 433 LPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGVTLQKYETL 492

Query: 493 KLIPKKR 496
           KLIPK+R
Sbjct: 493 KLIPKRR 497

BLAST of CmUC11G204140 vs. NCBI nr
Match: XP_008467223.1 (PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo])

HSP 1 Score: 677.6 bits (1747), Expect = 8.4e-191
Identity = 331/487 (67.97%), Postives = 396/487 (81.31%), Query Frame = 0

Query: 13  IFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQTYGPLI 72
           + LLLP+ FI+ K  +SP SK  P+PPGP PWPILGNLLQIG+NAHISIT  A  YGPLI
Sbjct: 13  LLLLLPI-FIILKHIKSPSSKL-PLPPGPNPWPILGNLLQIGQNAHISITQFANIYGPLI 72

Query: 73  SLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDSWR 132
           SL+ G + L+VASSPAAA AVLKTQDRLLS RY+F MTPDRA H + SLVFSPEC D W+
Sbjct: 73  SLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECGDQWK 132

Query: 133 SLRSICKANLFTARAIESQGSLRRRKMNEMMEFLLSKQGSTVEIRDFVLTTVFNILSNFI 192
           +LRS+CK NLFTA+AIESQ  LR +KM E+++FL SKQGS VE++DFV T+VFN LSN I
Sbjct: 133 NLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVFTSVFNTLSNLI 192

Query: 193 FSRDLFDFDGDGFNGIKQPFSNLKNLGLTPNLGDFYPFLTNLDIQGLRNKCSLYSKQINS 252
           FS DL DF GDGF GIK PF  + +LGLTPNL DFYP L   D+QGL+NK  +Y  +++S
Sbjct: 193 FSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKNEVDS 252

Query: 253 TFDILINERRKVSSPNSSSPQDFLDVLIQSQFTDDQISYLVIELLLAGTDTSTMTVEWAM 312
            + ILI ERR++     S+  DFLDVLIQS F D QI+ L+IELL AGTDT+T TVEWAM
Sbjct: 253 LWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGTDTTTTTVEWAM 312

Query: 313 AEVLKNKDIMEKVREEMKRETKQN-SIDETEVCKLKYLWKCVKETLRLHPPAPFLLPRIA 372
           +E+LK+KDI+ KVREEMK+ TK+N  IDE+ + +L+YLW+CVKETLRLHPPAPFLLPR+A
Sbjct: 313 SEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRLHPPAPFLLPRLA 372

Query: 373 PMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFS---DYDHVEFKGNDYGY 432
           PMDCE+MGYSIPKD+++FVN+WGIGRDPS+WED  +FNPQRF      D+V+FKG DY Y
Sbjct: 373 PMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVDFKGYDYRY 432

Query: 433 LPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQKKQPL 492
           LPFGGGRRICPG PMA +Q+PLILATLIHNFEWSLP+ ED SK+D+ G LGVTLQK + L
Sbjct: 433 LPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGVTLQKYETL 492

Query: 493 KLIPKKR 496
           KLIPK+R
Sbjct: 493 KLIPKRR 497

BLAST of CmUC11G204140 vs. ExPASy Swiss-Prot
Match: Q9FXW4 (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3442 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 1.1e-113
Identity = 219/489 (44.79%), Postives = 310/489 (63.39%), Query Frame = 0

Query: 9   VSVLIFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQTY 68
           + VL   ++   F++F  F    S+   +PPGP+P PI+GNLLQ+G   H     LAQ Y
Sbjct: 1   MEVLSIAIVSFSFLLFLFFILRDSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKY 60

Query: 69  GPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECN 128
           G L SL+ G + +VVASSPAAAA +LKT D++LSGRYVF     + +H E S+V+S ECN
Sbjct: 61  GELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVK-EHVENSIVWS-ECN 120

Query: 129 DSWRSLRSICKANLFTARAIESQGSLRRRKMNEMMEFLLSKQGSTVEIRDFVLTTVFNIL 188
           D+W+ LR +C+  LFT + IESQ  +R  K  EM++FL  K+G  V+I + V  T+ NI 
Sbjct: 121 DNWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIF 180

Query: 189 SNFIFSRDLFDFDG--DGFNGIKQPFSNLKNLGLTPNLGDFYPFLTNLDIQGLRNKCSLY 248
            N IFS+D+FD +    G   +K+    L ++G + N  D++P +  LD+ G R   +  
Sbjct: 181 GNLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEV 240

Query: 249 SKQINSTFDILINERRKVSSPNSSSPQDFLDVLIQSQFTDDQISYLVIELLLAGTDTSTM 308
            +QI   + +++ ERR   +  S S  DF+DVL+ +   D +I+ L++EL  AGT+TS  
Sbjct: 241 LQQIYDVWGVMLKERR--GTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSAS 300

Query: 309 TVEWAMAEVLKNKDIMEKVREEMKRETKQNSIDETEVCKLKYLWKCVKETLRLHPPAPFL 368
           T+EWA+ E+ K   ++ K+R E+      N++ E+++  L YL   VKETLRLHPP P L
Sbjct: 301 TIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTPLL 360

Query: 369 LPRIAPMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFSDYDHVEFKGNDY 428
           LPR A   C +M Y+IPK+  I VN W IGRDP  W+D L+F P+RF   D V++KGND+
Sbjct: 361 LPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSD-VDYKGNDF 420

Query: 429 GYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQKKQ 488
             +PFGGGRRICPG P+A+    LI+ATL+ NFEWSLP G   S++ M+   G+TLQK  
Sbjct: 421 ELIPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDP 480

Query: 489 PLKLIPKKR 496
           PL ++ K R
Sbjct: 481 PLLIVLKAR 484

BLAST of CmUC11G204140 vs. ExPASy Swiss-Prot
Match: O64900 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3467 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 4.9e-109
Identity = 206/477 (43.19%), Postives = 298/477 (62.47%), Query Frame = 0

Query: 22  IVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQTYGPLISLRFGGRLL 81
           I++ LF S   K   +PPGPKPWPI+GNLLQ+G   H     LAQTYG + +L+ G   +
Sbjct: 16  ILYLLFGSSGHKN--LPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETV 75

Query: 82  VVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDSWRSLRSICKAN 141
           VVAS+ +AA+ +LKT DR+LS RYVF     +  H E S+V+S +C ++W++LR +C+  
Sbjct: 76  VVASTSSAASEILKTHDRILSARYVFQSFRVKG-HVENSIVWS-DCTETWKNLRKVCRTE 135

Query: 142 LFTARAIESQGSLRRRKMNEMMEFLLSKQGSTVEIRDFVLTTVFNILSNFIFSRDLFDF- 201
           LFT + IESQ  +R +K  EM+E+L+ KQG  V+I + +  T+ NI  N IFS+++F+  
Sbjct: 136 LFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELG 195

Query: 202 -DGDGFNGIKQPFSNLKNLGLTPNLGDFYPFLTNLDIQGLRNKCSLYSKQINSTFDILIN 261
               G +  K+    +  LG + N  D++P L   D+ G R + +   K I + +  ++ 
Sbjct: 196 XPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQ 255

Query: 262 ERRKVSSPNS-SSPQDFLDVLIQSQFTDDQISYLVIELLLAGTDTSTMTVEWAMAEVLKN 321
           ER+     +   S  DF+DV + S   D QI+ L++EL  AGT+TS  T+EWAM E+ KN
Sbjct: 256 ERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMTELTKN 315

Query: 322 KDIMEKVREEMKRETKQNSIDETEVCKLKYLWKCVKETLRLHPPAPFLLPRIAPMDCELM 381
             I  K+R E++    + S+ E++   L YL   VKETLRLHPP P LLPR A   C ++
Sbjct: 316 PKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRALETCTIL 375

Query: 382 GYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFSDYDHVEFKGNDYGYLPFGGGRRIC 441
            Y+IPKD  I VN WGIGRDP  W D L+F+P+RF +   V+F+GND+  +PFG GRRIC
Sbjct: 376 NYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLN-SSVDFRGNDFSLIPFGAGRRIC 435

Query: 442 PGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQKKQPLKLIPKKR 496
           PG P+A   + L++AT + N +W LP+G     + +E   G+TLQK+ PL ++PK R
Sbjct: 436 PGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVPKSR 487

BLAST of CmUC11G204140 vs. ExPASy Swiss-Prot
Match: Q9SP06 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum OX=3469 GN=CYP80B3 PE=1 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 6.4e-109
Identity = 211/491 (42.97%), Postives = 307/491 (62.53%), Query Frame = 0

Query: 8   LVSVLIFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQT 67
           LV+V+I   L      + +FR   S    +PPGPKPWPI+GNLLQ+G   H     LA+T
Sbjct: 2   LVAVVITTFL------YLIFRD--SSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAET 61

Query: 68  YGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPEC 127
           YG L SL+ G   +VVAS+P AA+ +LKT DR+LSGRYVF     + +H E S+V+S EC
Sbjct: 62  YGDLFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVK-EHVENSIVWS-EC 121

Query: 128 NDSWRSLRSICKANLFTARAIESQGSLRRRKMNEMMEFLLSKQGSTVEIRDFVLTTVFNI 187
           N++W+ LR +C+  LFT + IESQ  +R  K  EM+E+L    G+ V+I + V  T+ NI
Sbjct: 122 NETWKKLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNI 181

Query: 188 LSNFIFSRDLFDFDGDGFNG---IKQPFSNLKNLGLTPNLGDFYPFLTNLDIQGLRNKCS 247
             N IFS+++F   GD  +G   +K+    +  LG + N  D++PFL   D+ G     +
Sbjct: 182 FGNLIFSQNIFKL-GDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVA 241

Query: 248 LYSKQINSTFDILINERRKVSSPNSSSPQDFLDVLIQSQFTDDQISYLVIELLLAGTDTS 307
              + I S +  ++ ER+     N+S   DF+++L+ S   D QI+ L++E+  AGT+TS
Sbjct: 242 DCLQGIYSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETS 301

Query: 308 TMTVEWAMAEVLKNKDIMEKVREEMKRETKQNSIDETEVCKLKYLWKCVKETLRLHPPAP 367
             T+EWA++E+ KN  +   +R E+     +  + E+++  + YL   VKETLRLHP  P
Sbjct: 302 ASTIEWALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATP 361

Query: 368 FLLPRIAPMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFSDYDHVEFKGN 427
            LLPR A   C+++ Y+IPK+  I VN WGIGRDP  W D L F+P+RF +   ++FKGN
Sbjct: 362 LLLPRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLN-SSIDFKGN 421

Query: 428 DYGYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQK 487
           D+  +PFG GRRICPG P+AT  + LI+++L+ NF+W  P G D S++ ME   G+TLQK
Sbjct: 422 DFELIPFGAGRRICPGVPLATQFISLIVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQK 480

Query: 488 KQPLKLIPKKR 496
           + PL ++PK R
Sbjct: 482 EPPLYIVPKTR 480

BLAST of CmUC11G204140 vs. ExPASy Swiss-Prot
Match: O64899 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica OX=3467 GN=CYP80B1 PE=1 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 1.2e-107
Identity = 208/491 (42.36%), Postives = 302/491 (61.51%), Query Frame = 0

Query: 8   LVSVLIFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQT 67
           L++V+I  +L L F          S    +PPGPKPWPI+GNLLQ+G   H     LAQT
Sbjct: 7   LIAVIISSILYLLF--------GGSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQT 66

Query: 68  YGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPEC 127
           YG + +L+ G   +VVAS+ +AA+ +LKT DR+LS RYVF     +  H E S+V+S +C
Sbjct: 67  YGDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKG-HVENSIVWS-DC 126

Query: 128 NDSWRSLRSICKANLFTARAIESQGSLRRRKMNEMMEFLLSKQGSTVEIRDFVLTTVFNI 187
            ++W++LR +C+  LFT + IESQ  +R +K  EM+E+L+ KQG  V+I + +  T+ NI
Sbjct: 127 TETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNI 186

Query: 188 LSNFIFSRDLFDF--DGDGFNGIKQPFSNLKNLGLTPNLGDFYPFLTNLDIQGLRNKCSL 247
             N IFS+++F+      G +  K+    +  LG + N  D++P L   D+ G R + + 
Sbjct: 187 FGNLIFSQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAE 246

Query: 248 YSKQINSTFDILINERRKVSSPNS-SSPQDFLDVLIQSQFTDDQISYLVIELLLAGTDTS 307
             K I + +  ++ ER+     +   S  DF+DV + S   D QI+ L++EL  AGT+TS
Sbjct: 247 CLKGIYAIWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETS 306

Query: 308 TMTVEWAMAEVLKNKDIMEKVREEMKRETKQNSIDETEVCKLKYLWKCVKETLRLHPPAP 367
             T+EWAM E+ KN  I  K+R E++    + S+ E++   L YL   VKETLRLHPP P
Sbjct: 307 ASTIEWAMTELTKNPKITAKIRSEIQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTP 366

Query: 368 FLLPRIAPMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFSDYDHVEFKGN 427
            LLPR A   C ++ Y+IPKD  I VN WGIGRDP  W D L+F+P+RF +   V+F+GN
Sbjct: 367 LLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLN-SSVDFRGN 426

Query: 428 DYGYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQK 487
           D+  +PFG GRRICPG P+A   + L++AT + N +W LP+G     + +E   G+TLQK
Sbjct: 427 DFSLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQK 486

Query: 488 KQPLKLIPKKR 496
           + PL ++PK R
Sbjct: 487 EPPLFIVPKSR 486

BLAST of CmUC11G204140 vs. ExPASy Swiss-Prot
Match: A8CDR5 (Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 8.7e-98
Identity = 193/492 (39.23%), Postives = 306/492 (62.20%), Query Frame = 0

Query: 9   VSVLIFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNA----HISITHL 68
           + + +F L+P+  +VF L   P  K   +PPGP PWP++GNL  +  N     HI++ ++
Sbjct: 3   LQIALFSLIPV-ILVFILLLKP--KYKNLPPGPHPWPLIGNLPILFTNTEVPLHITLANM 62

Query: 69  AQTYGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFS 128
           A+T+GP++ L  G +  V+AS+  AA  +LKT DR+ S R++  M+     H + SLV+S
Sbjct: 63  ARTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHI-RMSFRLKHHIKYSLVWS 122

Query: 129 PECNDSWRSLRSICKANLFTARAIESQGSLRRRKMNEMMEFLLSKQGSTVEIRDFVLTTV 188
            +C D W+ LR I +  +F+ + +++Q  +R +K+ E+++FL SK+G  V+I  FV  T+
Sbjct: 123 -DCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTL 182

Query: 189 FNILSNFIFSRDLFDF-DGDGFNGIKQPFSNLKNLGLTPNLGDFYPFLTNLDIQGLRNKC 248
            NIL N +FS+D+F + D     GI+     +  +G  PN+ +FYP L  LD+QGL+ KC
Sbjct: 183 LNILGNVVFSKDVFVYGDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKC 242

Query: 249 SLYSKQINSTFDILINERRKVSSPNSSSPQDFLDVLIQSQFTDDQISYLVIELLLAGTDT 308
                ++   ++  + ER+   +  +   +D LDVL+ + F D QI+ L +E    G++T
Sbjct: 243 DERFIRVMKMWEGTVKERK---ANRNEESKDMLDVLLANDFNDAQINALFLETFGPGSET 302

Query: 309 STMTVEWAMAEVLKNKDIMEKVREEMKRETKQNSIDETEVCKLKYLWKCVKETLRLHPPA 368
           S+ T+EW +AE++K+   M KVR+E+      ++I E+++ +L YL  C+KE +RLHP A
Sbjct: 303 SSATIEWVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAMRLHPAA 362

Query: 369 PFLLPRIAPMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFSDYDHVEFKG 428
           PFLLPR A   CE+MGY+IPK++ + VN + IGRDP  W+D  +F P+RF + D V+F G
Sbjct: 363 PFLLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLESD-VDFHG 422

Query: 429 NDYGYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQ 488
             Y ++PFG GRR C G P+AT  +PLI+ +L+HN+++ LP G     + M  ML +TL 
Sbjct: 423 AHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLSLTLA 482

Query: 489 KKQPLKLIPKKR 496
               L ++PK R
Sbjct: 483 IDPSLCVVPKAR 485

BLAST of CmUC11G204140 vs. ExPASy TrEMBL
Match: A0A6J1E237 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Momordica charantia OX=3673 GN=LOC111025729 PE=3 SV=1)

HSP 1 Score: 715.7 bits (1846), Expect = 1.3e-202
Identity = 355/497 (71.43%), Postives = 415/497 (83.50%), Query Frame = 0

Query: 2   ASQEEFLVSVLIFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISI 61
           A  +E  + + I LLL   FI  K FRSP SKQ  +PPGPKPWPILGNLLQIG +AH S+
Sbjct: 6   ADDQEQQLVLPILLLLASLFITLKYFRSPSSKQTKLPPGPKPWPILGNLLQIGEDAHASM 65

Query: 62  THLAQTYGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSL 121
           T  AQ YGPLISLR GG+LLVVASSP AAA VLKTQDRLLS RY+F    D A HRECS 
Sbjct: 66  TRFAQAYGPLISLRLGGQLLVVASSPDAAAVVLKTQDRLLSARYIFQTVHDEALHRECSF 125

Query: 122 VFSPECNDSWRSLRSICKANLFTARAIESQGSLRRRKMNEMMEFLLSKQGSTVEIRDFVL 181
           VFSPECND WR +RSIC+ANLFTA+AI+SQ +LRR KM+EM+EFL+SKQGS VEIRDFV 
Sbjct: 126 VFSPECNDRWRKVRSICRANLFTAKAIDSQANLRRMKMDEMVEFLVSKQGSVVEIRDFVF 185

Query: 182 TTVFNILSNFIFSRDLFDFDGDGFNGIKQPFSNLKNLGLTPNLGDFYPFLTNLDIQGLRN 241
           TTVFNILSNFIFSRDLFDF GDGFNGIK     +  LGLTPNL D+Y  L NLD+QGLR 
Sbjct: 186 TTVFNILSNFIFSRDLFDFVGDGFNGIKASLKKMVTLGLTPNLADYYSLLRNLDLQGLRK 245

Query: 242 KCSLYSKQINSTFDILINERRKVSSPNSSS--PQDFLDVLIQSQFTDDQISYLVIELLLA 301
           K ++Y KQI+S +  LI+ERRK+   NS +   +DFLDVLIQSQF D+QI++L++E+ LA
Sbjct: 246 KATIYRKQIDSAWKELIDERRKIHLQNSPNFVARDFLDVLIQSQFNDEQINHLIVEIFLA 305

Query: 302 GTDTSTMTVEWAMAEVLKNKDIMEKVREEMKRE-TKQNSIDETEVCKLKYLWKCVKETLR 361
           GTDT+TMTVEWAMAE+LK +DIM KVREEMKRE   Q+++DE+++ +L YLWKCVKETLR
Sbjct: 306 GTDTTTMTVEWAMAEMLKQRDIMAKVREEMKREINNQSTVDESKISQLHYLWKCVKETLR 365

Query: 362 LHPPAPFLLPRIAPMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFSDYDH 421
           LHPPAPFLLPR+AP DCELMGYSIPKDTLIFVN+WGIGRDPS+WED LSFNP+RF+D D 
Sbjct: 366 LHPPAPFLLPRVAPTDCELMGYSIPKDTLIFVNVWGIGRDPSLWEDSLSFNPERFTDCD- 425

Query: 422 VEFKGNDYGYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGML 481
           V+FKG DY YLPFGGGRRICPG PMA++Q+PLILATLIHNFE SLP+G+DFSK+D++G L
Sbjct: 426 VDFKGYDYRYLPFGGGRRICPGLPMASVQIPLILATLIHNFELSLPNGQDFSKLDLDGKL 485

Query: 482 GVTLQKKQPLKLIPKKR 496
           GVTLQKK+PL+LI +KR
Sbjct: 486 GVTLQKKKPLQLILQKR 501

BLAST of CmUC11G204140 vs. ExPASy TrEMBL
Match: A0A6J1ELA6 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435609 PE=3 SV=1)

HSP 1 Score: 678.3 bits (1749), Expect = 2.4e-191
Identity = 332/483 (68.74%), Postives = 392/483 (81.16%), Query Frame = 0

Query: 13  IFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQTYGPLI 72
           + LLLPL FI+ +   S  SKQPP+PPGPKPWPILGNLLQIG+NAHIS+T  +  YGPLI
Sbjct: 9   LLLLLPLIFIILRHI-SSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLI 68

Query: 73  SLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDSWR 132
           SL+ G + LVVASSPAAAAA+LKTQDRLLS RY+F MTPDR  H  CSL+FSPEC D W+
Sbjct: 69  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 128

Query: 133 SLRSICKANLFTARAIESQGSLRRRKMNEMMEFLLSKQGSTVEIRDFVLTTVFNILSNFI 192
           +LRSIC+A LFTARAIESQ SLRR+KMNEM+EFL SKQGS VEI++ V  TVFN LSNFI
Sbjct: 129 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 188

Query: 193 FSRDLFDFDGDGFNGIKQPFSNLKNLGLTPNLGDFYPFLTNLDIQGLRNKCSLYSKQINS 252
           FSRDL  F GDGFNGIK PF N+ +LGL PNL DFYP L   D+QGLR K ++Y  Q++S
Sbjct: 189 FSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQVDS 248

Query: 253 TFDILINERRKVSSPNSSSPQDFLDVLIQSQFTDDQISYLVIELLLAGTDTSTMTVEWAM 312
           +++ILI ERR + S   S+  DFLDVLIQS FTD+QISYL++E+L AGTDT+T TVEWAM
Sbjct: 249 SWEILIKERRTIHSQGVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTTTTTVEWAM 308

Query: 313 AEVLKNKDIMEKVREEMKRETKQNSIDETEVCKLKYLWKCVKETLRLHPPAPFLLPRIAP 372
           AE+LKN+DIM +VREE K+     +IDE+++ +L YLWKCVKETLRLHPPAPFLLPR AP
Sbjct: 309 AELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAP 368

Query: 373 MDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFSDYDHVEFKGNDYGYLPFG 432
             CELMGYS+PK T+IFVN+WGIGRDPSIWED  SF P+RF   D  + KG+DY +LPFG
Sbjct: 369 EACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCD-ADLKGHDYRFLPFG 428

Query: 433 GGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQKKQPLKLIP 492
           GGRRICPG PMA +Q+PLILATLIHNFEW LP+ ED S +D+ G LGVTLQK++PLKL+ 
Sbjct: 429 GGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLKLLS 488

Query: 493 KKR 496
           K R
Sbjct: 489 KNR 489

BLAST of CmUC11G204140 vs. ExPASy TrEMBL
Match: A0A5A7UIU3 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G001030 PE=3 SV=1)

HSP 1 Score: 677.6 bits (1747), Expect = 4.1e-191
Identity = 331/487 (67.97%), Postives = 396/487 (81.31%), Query Frame = 0

Query: 13  IFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQTYGPLI 72
           + LLLP+ FI+ K  +SP SK  P+PPGP PWPILGNLLQIG+NAHISIT  A  YGPLI
Sbjct: 13  LLLLLPI-FIILKHIKSPSSKL-PLPPGPNPWPILGNLLQIGQNAHISITQFANIYGPLI 72

Query: 73  SLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDSWR 132
           SL+ G + L+VASSPAAA AVLKTQDRLLS RY+F MTPDRA H + SLVFSPEC D W+
Sbjct: 73  SLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECGDQWK 132

Query: 133 SLRSICKANLFTARAIESQGSLRRRKMNEMMEFLLSKQGSTVEIRDFVLTTVFNILSNFI 192
           +LRS+CK NLFTA+AIESQ  LR +KM E+++FL SKQGS VE++DFV T+VFN LSN I
Sbjct: 133 NLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVFTSVFNTLSNLI 192

Query: 193 FSRDLFDFDGDGFNGIKQPFSNLKNLGLTPNLGDFYPFLTNLDIQGLRNKCSLYSKQINS 252
           FS DL DF GDGF GIK PF  + +LGLTPNL DFYP L   D+QGL+NK  +Y  +++S
Sbjct: 193 FSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKNEVDS 252

Query: 253 TFDILINERRKVSSPNSSSPQDFLDVLIQSQFTDDQISYLVIELLLAGTDTSTMTVEWAM 312
            + ILI ERR++     S+  DFLDVLIQS F D QI+ L+IELL AGTDT+T TVEWAM
Sbjct: 253 LWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGTDTTTTTVEWAM 312

Query: 313 AEVLKNKDIMEKVREEMKRETKQN-SIDETEVCKLKYLWKCVKETLRLHPPAPFLLPRIA 372
           +E+LK+KDI+ KVREEMK+ TK+N  IDE+ + +L+YLW+CVKETLRLHPPAPFLLPR+A
Sbjct: 313 SEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRLHPPAPFLLPRLA 372

Query: 373 PMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFS---DYDHVEFKGNDYGY 432
           PMDCE+MGYSIPKD+++FVN+WGIGRDPS+WED  +FNPQRF      D+V+FKG DY Y
Sbjct: 373 PMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVDFKGYDYRY 432

Query: 433 LPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQKKQPL 492
           LPFGGGRRICPG PMA +Q+PLILATLIHNFEWSLP+ ED SK+D+ G LGVTLQK + L
Sbjct: 433 LPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGVTLQKYETL 492

Query: 493 KLIPKKR 496
           KLIPK+R
Sbjct: 493 KLIPKRR 497

BLAST of CmUC11G204140 vs. ExPASy TrEMBL
Match: A0A1S3CT14 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656 GN=LOC103504626 PE=3 SV=1)

HSP 1 Score: 677.6 bits (1747), Expect = 4.1e-191
Identity = 331/487 (67.97%), Postives = 396/487 (81.31%), Query Frame = 0

Query: 13  IFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQTYGPLI 72
           + LLLP+ FI+ K  +SP SK  P+PPGP PWPILGNLLQIG+NAHISIT  A  YGPLI
Sbjct: 13  LLLLLPI-FIILKHIKSPSSKL-PLPPGPNPWPILGNLLQIGQNAHISITQFANIYGPLI 72

Query: 73  SLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDSWR 132
           SL+ G + L+VASSPAAA AVLKTQDRLLS RY+F MTPDRA H + SLVFSPEC D W+
Sbjct: 73  SLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECGDQWK 132

Query: 133 SLRSICKANLFTARAIESQGSLRRRKMNEMMEFLLSKQGSTVEIRDFVLTTVFNILSNFI 192
           +LRS+CK NLFTA+AIESQ  LR +KM E+++FL SKQGS VE++DFV T+VFN LSN I
Sbjct: 133 NLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVFTSVFNTLSNLI 192

Query: 193 FSRDLFDFDGDGFNGIKQPFSNLKNLGLTPNLGDFYPFLTNLDIQGLRNKCSLYSKQINS 252
           FS DL DF GDGF GIK PF  + +LGLTPNL DFYP L   D+QGL+NK  +Y  +++S
Sbjct: 193 FSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKNEVDS 252

Query: 253 TFDILINERRKVSSPNSSSPQDFLDVLIQSQFTDDQISYLVIELLLAGTDTSTMTVEWAM 312
            + ILI ERR++     S+  DFLDVLIQS F D QI+ L+IELL AGTDT+T TVEWAM
Sbjct: 253 LWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGTDTTTTTVEWAM 312

Query: 313 AEVLKNKDIMEKVREEMKRETKQN-SIDETEVCKLKYLWKCVKETLRLHPPAPFLLPRIA 372
           +E+LK+KDI+ KVREEMK+ TK+N  IDE+ + +L+YLW+CVKETLRLHPPAPFLLPR+A
Sbjct: 313 SEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRLHPPAPFLLPRLA 372

Query: 373 PMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFS---DYDHVEFKGNDYGY 432
           PMDCE+MGYSIPKD+++FVN+WGIGRDPS+WED  +FNPQRF      D+V+FKG DY Y
Sbjct: 373 PMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVDFKGYDYRY 432

Query: 433 LPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQKKQPL 492
           LPFGGGRRICPG PMA +Q+PLILATLIHNFEWSLP+ ED SK+D+ G LGVTLQK + L
Sbjct: 433 LPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGVTLQKYETL 492

Query: 493 KLIPKKR 496
           KLIPK+R
Sbjct: 493 KLIPKRR 497

BLAST of CmUC11G204140 vs. ExPASy TrEMBL
Match: A0A6J1I4V7 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=3661 GN=LOC111469167 PE=3 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 2.0e-190
Identity = 329/483 (68.12%), Postives = 394/483 (81.57%), Query Frame = 0

Query: 13  IFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQTYGPLI 72
           + LLLPL F + +   S  SKQPP+PPGP PWPILGNLLQIG+NAHIS+T  +  YGPLI
Sbjct: 9   LLLLLPLIFTILRHICS-SSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHFYGPLI 68

Query: 73  SLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDSWR 132
           SL+ G + LVVASSPAAAAA+LKTQDRLLS RY+F MTPDR  H  CSL+FSPEC D W+
Sbjct: 69  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 128

Query: 133 SLRSICKANLFTARAIESQGSLRRRKMNEMMEFLLSKQGSTVEIRDFVLTTVFNILSNFI 192
           +LRSIC+A LFTARAIESQ SLRR+KMN+M+EFL SKQGS VEI++ V  TVFN LSNFI
Sbjct: 129 NLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 188

Query: 193 FSRDLFDFDGDGFNGIKQPFSNLKNLGLTPNLGDFYPFLTNLDIQGLRNKCSLYSKQINS 252
           FSRDL  F GDGFNGIK  F N+ +LGL PNL DFYP L + D+QGLR K ++Y  Q++S
Sbjct: 189 FSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAAIYKNQVDS 248

Query: 253 TFDILINERRKVSSPNSSSPQDFLDVLIQSQFTDDQISYLVIELLLAGTDTSTMTVEWAM 312
           +++ILI ERR + S + S+  DFLDVLI+S FTD+QISYL++E+L AGTDT+T TVEWAM
Sbjct: 249 SWEILIKERRTIHSRDVSAASDFLDVLIESGFTDEQISYLIVEVLSAGTDTTTTTVEWAM 308

Query: 313 AEVLKNKDIMEKVREEMKRETKQNSIDETEVCKLKYLWKCVKETLRLHPPAPFLLPRIAP 372
           AE+LKN+DIM +VREE K+     +IDE+++ +L YLWKCVKETLRLHPPAPFLLPR AP
Sbjct: 309 AELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAP 368

Query: 373 MDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFSDYDHVEFKGNDYGYLPFG 432
             CELMGYS+PKDT+IFVN+WGIGRDPSIWED  SF P+RF  +D  + KG+DY +LPFG
Sbjct: 369 EACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFD-ADLKGHDYRFLPFG 428

Query: 433 GGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQKKQPLKLIP 492
           GGRRICPG PMA +Q+PLILATLIHNFEW LP+ ED S +D+ G LGVTLQK++PLKL+ 
Sbjct: 429 GGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKEEPLKLLS 488

Query: 493 KKR 496
           KKR
Sbjct: 489 KKR 489

BLAST of CmUC11G204140 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 344.4 bits (882), Expect = 1.6e-94
Identity = 194/503 (38.57%), Postives = 295/503 (58.65%), Query Frame = 0

Query: 13  IFLLLPLFFIVFKLFRSPCS--KQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQTYGP 72
           +F  +  FFI+F     P S  K  P PPGP   PI+GN+  +GRN H S   L++TYGP
Sbjct: 12  LFCFVLSFFIIFFTTTRPRSSRKVVPSPPGPPRLPIIGNIHLVGRNPHHSFADLSKTYGP 71

Query: 73  LISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRA----QHRECSLVFSPE 132
           ++SL+FG    VV +SP AA  VL+T D++LS R     TP  +     H + S+V+ P 
Sbjct: 72  IMSLKFGSLNTVVVTSPEAAREVLRTYDQILSSR-----TPTNSIRSINHDKVSVVWLPP 131

Query: 133 CNDSWRSLRSICKANLFTARAIESQGSLRRRKMNEMMEFL--LSKQGSTVEIRDFVLTTV 192
            +  WR LR +    LF+ + IE+  +LR  K+ E++ F+   S++   V+I      T 
Sbjct: 132 SSSRWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREEAVDISRATFITA 191

Query: 193 FNILSNFIFSRDLFDFDGDGFNGIKQPFSNLKNLGLTPNLGDFYPFLTNLDIQGLRNKCS 252
            NI+SN +FS DL ++D +     +     +      P+  +F+PFL  LD+QG R    
Sbjct: 192 LNIISNILFSVDLGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRKTLK 251

Query: 253 LYSKQINSTFDILINER---RKVSSPNSSS--PQDFLDVLI------QSQFTDDQISYLV 312
             S+++   F   I+ +   + +   NS     +DF+DVL+      +++   + I +L+
Sbjct: 252 ACSERLFKVFRGFIDAKLAEKSLRDTNSKDVRERDFVDVLLDLTEGDEAELNTNDIVHLL 311

Query: 313 IELLLAGTDTSTMTVEWAMAEVLKNKDIMEKVREEMKRETKQNS-IDETEVCKLKYLWKC 372
           ++L  AGTDT++ TVEWAMAE+L+N + M K + E+     Q   ++E+++  L YL   
Sbjct: 312 LDLFGAGTDTNSSTVEWAMAELLRNPETMVKAQAEIDCVIGQKGVVEESDISALPYLQAV 371

Query: 373 VKETLRLHPPAPFLLPRIAPMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQR 432
           VKET RLHP AP L+PR A  D E++G+ +PKDT +FVN+W IGRDP++WE+   F P+R
Sbjct: 372 VKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIGRDPNVWENSSRFKPER 431

Query: 433 FSDYDHVEFKGNDYGYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKV 492
           F   D ++ +G DY   PFG GRRICPG P+A   +PL+LA+L+++F+W LP+G     +
Sbjct: 432 FLGKD-IDLRGRDYELTPFGAGRRICPGLPLAVKTVPLMLASLLYSFDWKLPNGVGSEDL 491

Query: 493 DMEGMLGVTLQKKQPLKLIPKKR 496
           DM+   G+TL K  PL  +P K+
Sbjct: 492 DMDETFGLTLHKTNPLHAVPVKK 508

BLAST of CmUC11G204140 vs. TAIR 10
Match: AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )

HSP 1 Score: 339.0 bits (868), Expect = 6.6e-93
Identity = 193/502 (38.45%), Postives = 294/502 (58.57%), Query Frame = 0

Query: 12  LIFLLLPLFFIVFKLFRSPCS--KQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQTYG 71
           L+F  +   F++    RS  S  +   +PPGP   PI+GN+ Q+G+N H S   LA+ YG
Sbjct: 11  LLFCFISSCFLISTTARSRRSSGRAATLPPGPPRLPIIGNIHQVGKNPHSSFADLAKIYG 70

Query: 72  PLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQ---HRECSLVFSPE 131
           P++SL+FG    VV +SP AA  VL+T D++LSGR     + D  +   H E S+++ P 
Sbjct: 71  PIMSLKFGCLNSVVITSPEAAREVLRTHDQILSGR----KSNDSIRCFGHEEVSVIWLPP 130

Query: 132 CNDSWRSLRSICKANLFTARAIESQGSLRRRKMNEMMEFL--LSKQGSTVEIRDFVLTTV 191
            +  WR LR +    +F+ +  E+  +LR +K+ E++ F+   S++   V+I     TTV
Sbjct: 131 SSARWRMLRKLSVTLMFSPQRTEATKALRMKKVQELVSFMNESSERKEAVDISRASYTTV 190

Query: 192 FNILSNFIFSRDLFDFDGDGFNGIKQPFSNLKNLGLTPNLGDFYPFLTNLDIQGLRNKCS 251
            NI+SN +FS DL  +D    N  +            P+  +++PF+  LD+QG R    
Sbjct: 191 LNIISNILFSVDLGSYDSKKSNEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAMR 250

Query: 252 LYSKQINSTF----DILINERRKVSSPNSSSPQDFLDVLI------QSQFTDDQISYLVI 311
             ++++   F    D  I E+   +     S +DFLD L+      +++  ++ I +L++
Sbjct: 251 GLTERLFRVFRGFMDAKIAEKSLGNYSKDVSNRDFLDSLLILNEGDEAELDNNDIEHLLL 310

Query: 312 ELLLAGTDTSTMTVEWAMAEVLKNKDIMEKVREEMKRETKQNS-IDETEVCKLKYLWKCV 371
           ++  AGTDTS+ T+EWAMAE+L+N   M K + EM R   QNS + E+++  L YL   V
Sbjct: 311 DMFTAGTDTSSSTLEWAMAELLRNPKTMVKAQAEMDRVLGQNSVVQESDISGLPYLQAVV 370

Query: 372 KETLRLHPPAPFLLPRIAPMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRF 431
           KET RLHP AP L+PR A  D E++G+ +PKDT + VN+W IGRDPS+WE+   F P+RF
Sbjct: 371 KETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVLVNVWAIGRDPSVWENPSQFEPERF 430

Query: 432 SDYDHVEFKGNDYGYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVD 491
              D ++ KG DY   PFGGGRRICPG P+A   + L+LA+L+++F+W LP+G     +D
Sbjct: 431 MGKD-IDVKGRDYELTPFGGGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLD 490

Query: 492 MEGMLGVTLQKKQPLKLIPKKR 496
           M+   G+TL +   L  IP K+
Sbjct: 491 MDETFGITLHRTNTLYAIPVKK 507

BLAST of CmUC11G204140 vs. TAIR 10
Match: AT1G33720.1 (cytochrome P450, family 76, subfamily C, polypeptide 6 )

HSP 1 Score: 337.8 bits (865), Expect = 1.5e-92
Identity = 198/508 (38.98%), Postives = 292/508 (57.48%), Query Frame = 0

Query: 1   MASQEEFLVSVLIFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHIS 60
           ++ Q  FL+   I   L LFF   +  RSPC      PPGP   PI+GN+  +G+N H S
Sbjct: 4   ISGQPLFLIFCFILSCL-LFFTTARSRRSPCQLSKS-PPGPPRLPIIGNIHLVGKNPHHS 63

Query: 61  ITHLAQTYGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECS 120
            T L++TYGP++SL+ G    VV +S  A   VLKT D++LSGRY+   T     H E S
Sbjct: 64  FTDLSKTYGPVMSLKLGCLNSVVIASRDAVREVLKTHDQILSGRYISEATKSN-NHHEFS 123

Query: 121 LVFSPECNDSWRSLRSICKANLFTARAIESQGSLRRRKMNEMMEFLLS--KQGSTVEIRD 180
           + +    +  +R LR +    LF+ + I++  +LR +K+ E++ FL    ++   V+I  
Sbjct: 124 VGWIHPSSSRFRMLRKLSATQLFSPQCIQATKALRMKKVQELVNFLSESCEREEAVDISH 183

Query: 181 FVLTTVFNILSNFIFSRDLFDFDGDGFNGIKQPFSNLKNLGLTPNLGDFYPFLTNLDIQG 240
               T  NI+SN +FS +L  +D    +  ++     +     P+L +F+PF+  LD+QG
Sbjct: 184 VSFVTALNIISNILFSVNLGSYDSKNSSAFQEMVIGYQESIGNPDLANFFPFMRFLDLQG 243

Query: 241 LRNKCSLYSKQINSTF----DILINERRKVSSPNSSSPQDFLDVLIQSQFTD------DQ 300
              K    S ++   F    D  I E+   S     S +DFLDVLI  Q  D      D+
Sbjct: 244 NSKKMRESSGRLLQVFREFYDARIVEKSSRSVEKDVSSKDFLDVLIDLQQGDETEINIDE 303

Query: 301 ISYLVIELLLAGTDTSTMTVEWAMAEVLKNKDIMEKVREEMKRETKQN-SIDETEVCKLK 360
           I +L++++ +AGTDT++ TVEWAMAE+L N   M KV++E+     QN    E+++ KL 
Sbjct: 304 IEHLLLDMFVAGTDTNSSTVEWAMAELLGNPKTMTKVQDEINHVIGQNGDFQESDISKLP 363

Query: 361 YLWKCVKETLRLHPPAPFLLPRIAPMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLS 420
           YL   VKET RLHP APFLL R A  + E++G+++ KD+ + VN+W IGRDP +WE+   
Sbjct: 364 YLKAVVKETFRLHPAAPFLLQRKAETNVEILGFTVLKDSQVLVNVWAIGRDPLVWENPTH 423

Query: 421 FNPQRFSDYDHVEFKGNDYGYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGE 480
           F P+RF   + ++ KG DY   PFG GRRICPG P+A   + L+LA+L++ FEW LP+G 
Sbjct: 424 FEPERFLGKE-IDVKGTDYELTPFGAGRRICPGLPLAMKTVHLMLASLLYTFEWKLPNGV 483

Query: 481 DFSKVDMEGMLGVTLQKKQPLKLIPKKR 496
               +DME   G+T+ K  PL  +P K+
Sbjct: 484 GSEDLDMEETFGLTVHKTNPLLAVPLKK 507

BLAST of CmUC11G204140 vs. TAIR 10
Match: AT2G45560.1 (cytochrome P450, family 76, subfamily C, polypeptide 1 )

HSP 1 Score: 329.7 bits (844), Expect = 4.0e-90
Identity = 187/504 (37.10%), Postives = 293/504 (58.13%), Query Frame = 0

Query: 11  VLIFLLLPLFFIVFKLFRSPCSKQ--PPIPPGPKPWPILGNLLQIGRNAHISITHLAQTY 70
           +L+F  +   F++F   RS    +    +PPGP   PI+GN+  +G++ H S   L++TY
Sbjct: 10  LLLFCFILSCFLIFTTTRSGRISRGATALPPGPPRLPIIGNIHLVGKHPHRSFAELSKTY 69

Query: 71  GPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRA----QHRECSLVFS 130
           GP++SL+ G    VV +SP AA  VL+T D++LS R     +P  A     H++ SLV+ 
Sbjct: 70  GPVMSLKLGSLNTVVIASPEAAREVLRTHDQILSAR-----SPTNAVRSINHQDASLVWL 129

Query: 131 PECNDSWRSLRSICKANLFTARAIESQGSLRRRKMNEMMEFL--LSKQGSTVEIRDFVLT 190
           P  +  WR LR +    L + + IE+  +LR  K+ E++ F+   S +  +V+I      
Sbjct: 130 PSSSARWRLLRRLSVTQLLSPQRIEATKALRMNKVKELVSFISESSDREESVDISRVAFI 189

Query: 191 TVFNILSNFIFSRDLFDFDGD-GFNGIKQPFSNLKNLGLTPNLGDFYPFLTNLDIQGLRN 250
           T  NI+SN +FS DL  ++     NG++    ++ +   TP+  +++PFL  LD+QG   
Sbjct: 190 TTLNIISNILFSVDLGSYNAKASINGVQDTVISVMDAAGTPDAANYFPFLRFLDLQGNVK 249

Query: 251 KCSLYSKQINSTF----DILINERRKVSSPNSSSPQDFLDVLI-----QSQFTDDQISYL 310
              + ++++   F    D  I E+   ++P   S  DF+D L+     +S+ +   I +L
Sbjct: 250 TFKVCTERLVRVFRGFIDAKIAEKSSQNNPKDVSKNDFVDNLLDYKGDESELSISDIEHL 309

Query: 311 VIELLLAGTDTSTMTVEWAMAEVLKNKDIMEKVREEMKRETKQNSI-DETEVCKLKYLWK 370
           ++++  AGTDTS+ T+EWAM E+LKN   M K + E+     QN I +E+++ KL YL  
Sbjct: 310 LLDMFTAGTDTSSSTLEWAMTELLKNPKTMAKAQAEIDCVIGQNGIVEESDISKLPYLQA 369

Query: 371 CVKETLRLHPPAPFLLPRIAPMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQ 430
            VKET RLH P P L+PR A  D E++G+ + KDT + VN+W IGRDPS+W++   F P+
Sbjct: 370 VVKETFRLHTPVPLLIPRKAESDAEILGFMVLKDTQVLVNVWAIGRDPSVWDNPSQFEPE 429

Query: 431 RFSDYDHVEFKGNDYGYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSK 490
           RF   D ++ +G DY   PFG GRRICPG P+A   + L+LA+L+++F+W LP G     
Sbjct: 430 RFLGKD-MDVRGRDYELTPFGAGRRICPGMPLAMKTVSLMLASLLYSFDWKLPKGVLSED 489

Query: 491 VDMEGMLGVTLQKKQPLKLIPKKR 496
           +DM+   G+TL K  PL  +P K+
Sbjct: 490 LDMDETFGLTLHKTNPLHAVPVKK 507

BLAST of CmUC11G204140 vs. TAIR 10
Match: AT3G52970.1 (cytochrome P450, family 76, subfamily G, polypeptide 1 )

HSP 1 Score: 317.4 bits (812), Expect = 2.1e-86
Identity = 175/497 (35.21%), Postives = 279/497 (56.14%), Query Frame = 0

Query: 19  LFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQI-GRNAHISITHLAQTYGPLISLRFG 78
           L ++    +   C  +  +PPGP PWP++GN+ Q+ G   H S+T L++ +GP+++LR G
Sbjct: 20  LIYVTCLFYTKRCRTR--LPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHGPIMTLRIG 79

Query: 79  GRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDSWRSLRSI 138
             L VV SS   A  + K  D  L+GR ++       +  + SL+ + +    WR LR +
Sbjct: 80  SMLTVVISSSEVAREIFKKHDAALAGRKIYEAMKG-GKSSDGSLI-TAQYGAYWRMLRRL 139

Query: 139 CKANLFTARAIESQGSLRRRKMNEMMEFLLSKQG-----STVEIRDFVLTTVFNILSNFI 198
           C    F  R +++   +R R +++M+ F+  ++G      T+++  +     FN++ N +
Sbjct: 140 CTTQFFVTRRLDAMSDVRSRCVDQMLRFV--EEGGQNGTKTIDVGRYFFLMAFNLIGNLM 199

Query: 199 FSRDLFDFDGDGFNGIKQPFSNLKNLGLTPNLGDFYPFLTNLDIQGLRNKCSLYSKQINS 258
           FSRDL D D    +        +      PN+ DF+P L  LD QG+R K   + ++   
Sbjct: 200 FSRDLLDPDSKRGSEFFYHTGKVMEFAGKPNVADFFPLLRFLDPQGIRRKTQFHVEKAFE 259

Query: 259 TFDILINERRKVSSPNSS--SPQDFLDVLIQ-----------SQFTDDQISYLVIELLLA 318
                I ER +V     S    +D+LDVL++           S F+   I+ +V E+  A
Sbjct: 260 IAGEFIRERTEVREREKSDEKTKDYLDVLLEFRGGDGVDEEPSSFSSRDINVIVFEMFTA 319

Query: 319 GTDTSTMTVEWAMAEVLKNKDIMEKVREEMKRETKQNS--IDETEVCKLKYLWKCVKETL 378
           GTDT+T T+EWA+AE+L N   + K++ E++   K ++  + E ++  L YL   + ETL
Sbjct: 320 GTDTTTSTLEWALAELLHNPRTLTKLQTELRTYFKSSNQKLQEEDLPNLPYLSAVIMETL 379

Query: 379 RLHPPAPFLLPRIAPMDCELMG-YSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRF-SD 438
           RLHPP PFL+P  A   C +   Y+IPK+T + VN+W IGRDP  W D + F P+RF SD
Sbjct: 380 RLHPPLPFLVPHKAMSTCHIFDQYTIPKETQVLVNVWAIGRDPKTWIDPIMFKPERFISD 439

Query: 439 YDHVEFKGNDYGYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDME 493
            D  +FKG DY +LPFG GRR+CP  P+A+  LPL + +++ +F+W+L +G +  ++DM 
Sbjct: 440 PDARDFKGQDYEFLPFGSGRRMCPALPLASRVLPLAIGSMVRSFDWALENGLNAEEMDMG 499

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038892964.15.5e-24383.70probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Benincasa hispida][more]
XP_022159319.12.8e-20271.43probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Momordica charantia][more]
XP_022928791.14.9e-19168.74probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata][more]
KAA0055170.18.4e-19167.97putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo var. maku... [more]
XP_008467223.18.4e-19167.97PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis mel... [more]
Match NameE-valueIdentityDescription
Q9FXW41.1e-11344.79Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3... [more]
O649004.9e-10943.19(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3... [more]
Q9SP066.4e-10942.97(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum... [more]
O648991.2e-10742.36(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia calif... [more]
A8CDR58.7e-9839.23Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1E2371.3e-20271.43probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Momordica charantia ... [more]
A0A6J1ELA62.4e-19168.74probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O... [more]
A0A5A7UIU34.1e-19167.97Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. ma... [more]
A0A1S3CT144.1e-19167.97probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656... [more]
A0A6J1I4V72.0e-19068.12probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=... [more]
Match NameE-valueIdentityDescription
AT2G45570.11.6e-9438.57cytochrome P450, family 76, subfamily C, polypeptide 2 [more]
AT2G45550.16.6e-9338.45cytochrome P450, family 76, subfamily C, polypeptide 4 [more]
AT1G33720.11.5e-9238.98cytochrome P450, family 76, subfamily C, polypeptide 6 [more]
AT2G45560.14.0e-9037.10cytochrome P450, family 76, subfamily C, polypeptide 1 [more]
AT3G52970.12.1e-8635.21cytochrome P450, family 76, subfamily G, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 312..342
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 11..467
e-value: 4.3E-18
score: 63.3
NoneNo IPR availablePANTHERPTHR47956:SF23(S)-N-METHYLCOCLAURINE 3'-HYDROXYLASE ISOZYME 1-LIKEcoord: 12..493
NoneNo IPR availablePANTHERPTHR47956CYTOCHROME P450 71B11-RELATEDcoord: 12..493
IPR001128Cytochrome P450PRINTSPR00385P450coord: 351..362
score: 39.99
coord: 438..449
score: 32.17
coord: 429..438
score: 60.95
coord: 299..316
score: 31.31
IPR001128Cytochrome P450PFAMPF00067p450coord: 38..485
e-value: 7.4E-100
score: 334.9
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 308..334
score: 24.87
coord: 428..438
score: 55.82
coord: 350..368
score: 32.62
coord: 65..84
score: 33.74
coord: 288..305
score: 31.18
coord: 183..201
score: 21.51
coord: 438..461
score: 33.44
coord: 391..415
score: 34.05
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 23..495
e-value: 3.1E-114
score: 384.2
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 38..495
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 431..440

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmUC11G204140.1CmUC11G204140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen