CmUC10G201130 (gene) Watermelon (USVL531) v1

Overview
NameCmUC10G201130
Typegene
OrganismCitrullus mucosospermus (Watermelon (USVL531) v1)
Description4-coumarate-CoA ligase
LocationCmU531Chr10: 33079074 .. 33083748 (+)
RNA-Seq ExpressionCmUC10G201130
SyntenyCmUC10G201130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAAAGTCAAATTTCAGTTTCATGTACTGTGGATAAAACCAAATCTTTCAACCCCCAAATTCCAAACCATCGTTTTTCTTCAATCCCATTTTAGCAATGGCGATCACGCTGCGCAAATCTTTCAATTCCCAATCGCAGATTTACACTTCCCGTCGTCCTCCAATCCACTTTCCGACCGACCCCACAATCTCCATTCCCTCATTCCTCTTTCGAAACTCCTCCTCTTTCCCTAACGCCCTCGCCCTCGCCGACTCCGACTCCGGCGAATCCTTAACTTTCCGTCAGCTTCAAATACAAGTCTCCAAGCTCGCCCATGTCTTCATCCAACTCGGGATTCGAAAAAGCGATGTGGTTCTCATATTCGCCCCTAATTCCATTCACTTCCCTGTTTGTTTCTTCGCCATTGTCGCCATTGGAGCCATCGCCACCACTTGTAATCCTGCTTACACTTTTGGGGAACTGTCAAAGCAAGTCGCTAACTGCAACCCCAAGCTCGTAATCACCGTTCCCGAACTCTGGGACACAATCGGTAAACTCAACTTGCCTTCTATAATTCTGGGATCTAAAATTTCTTCAAAATTTTCCCGTCCCAACATCTGGGGTTACTCCGATTTGATTAAAAAAGCCGGCGATGTGTCCAATTTGCCGGTGAGTGAAGTGGGTCAGAACGATGTAGCGGCGCTTTTATATTCATCTGGGACGACTGGGATTAGTAAGGGAGTGATTCTAACTCACAGGAACTTCATAACTACCTCTTTAATGGTGACCCAAGATCAGGATCTTCTTGGTGATCCTAGAAACGTGTTTTTATGCTTTCTTCCAATGTTCCATGTGTTTGGTCTCTCTATAATTGTTTACTCGCAGCTTCAAAGAGGAAACACTGTCGTTTCAATGGCGAAGTTTGAGCTAGAAAAGGCTTTGGGGGTGGTGATGAAGTACAGGATCACTCATCTTTATGTTGTTCCACCTGTAATTATAGCTCTCACAAAGCAAAGTGTAGTGAAGAACTACGACTTGTCGTCGTTGAAGCAGATTTTATCTGGGGCAGCGCCTCTTGGGAAGGATGTGATGGAAGAATGCTCCAAGTTTATACCTCAGGCCAGGATCATCCAGGTTTTAAACTCTGCATTTTCAATTCCATTTTTCATTTTCATTTGCTTCTTTCACAAAGTATTATATTATCTTAAGATGATTGTTGTGTGCAGGAAGTTTAATATTATAAGTGCATCTCGGGGTAGTACCTATTTTTGTGCAACCAAATTATCCAATCATAATTAGTCACATAACAAATTCATCTTACTTTATTTTATAAATATTTACATTCGTTCTTTTACGTGTGTGATGAGTATGGGATGCATAAAGATGGATGCACCTTAAGATGTACTCAAGTATTGCTCGATATACTAAATATATGTTTGTCCATATCTTTTGTCTCTTGGTTTTCTATGCTGCTGGTCCAAATTGGCATGCAGCTACTGTAATAAGAGGGTGAGTCAAAGTAGCACTTCTTAGAGTGATAAAACAGTGCTTTTATTCTATTAAAAGCATCTTTCATTACATTCTCAACAGTAACTCTTGAAAATGTACTCTTTACAAGAGTGTTGGGAGGAAAAAAATGAACTTAAAAGGATGTTTGGCTGAGTGGGTTATTACAACCCACTCTGTGTTTGTGACACCAATTATAATAGTTGGTGTTTCCAACTATTATAACTTACAATTAGCTACAATATTATTATTTCAAATCCCTCTTTGCTATAGTGTTTGTTATTTCCTACTCATCCTCTCTTTCCCTCCTTGATACAATGTTTGGTATTTTCTATCCATACTAAAATAGTATGCGCCTCATACACAAACACACCGACTAAATTAGTTGGGACCCCAAATACTGACAATTTTAACCTACCGACTATTGTAACCCACAAACTCTAATAACCACTAGGTCTAATAACCAACTCAGCACCTCAAACGTCCTCTAAATTTGTTTTTGAAACCTTCTACTAGGATGGGTTCAGTGACATAAATCATGCAAAAACAAAACTAATGTGCCCAACTTAGCATACATTTTAGTTTTCCACCTCTTTAAACTTGAATGTGAGGAATAGTAAAAAGGAATATAGAACAACTTAAATCCTGAATGGTTCCTTTAGTGGGGGCCAGTGTTGTTCTGCTCCTTCCTGTGATATTTTGTATGGTGTGTTTGTTTCCTAAATAGTCTTGATTTGGGTTGCCATTTATGCTCTACAGGGATATGGCATGACAGAAACTTGTGGAGTTATTTCAGTGGGGAATGTGGGAGTAGAATCTACGCTATCTGGGGCAACTGGATCCCTTGTTTCAGGAGTTGAAGCGCAAGTTTTGAGTACAGAAACACAGAAACGTCTTCCACCCGGTGAAACAGGGGAAATTTGTGTGCGAGGACCAAATATGATGAAAGGTGGATCTCCTTGGACGTCTCTCTCTCCCTTATGGGATTTTGGAAATTGCTAGTAAAAAAGATTAACGTTATTAAACACATTAAACTCACTATACTTTCATAAGGAACCAAGCTTTATTGAGGAATAATAAAAGAATACAAGTGCATACGAAAAGGCAGCCTATAAAAACTGAGTCAAACTAAAATAAAAATGGATGTCTAGCTAAATAAGGCCTAAAGGATGAGTACAAAAATCGTTGTGCACTGTGGCCCAACCTTCTTCCAAGAACATCTTTTTGTTCTGATGAATAGCACTCATCCATACTAAATTACTAATTATGCTGAAGGGTGCATAAATGATCAAACAGCAACCTAGAATTATTATACCTTGACCCAATCTCCTCATTTGAAGATTCTTATTGATTGTGCTTGCTTATTCATTTTCATTTATTTTATTATGTTATTTTCCTGTACTTGCTTAACTGTATCTGCTGAAAATAATAAAAATTGCTTCCGGTTTTAAAGAAGCCAATATCATTTCTTTGATAATTTGTTGTTAGATTATTCAAGTTTGGACCTATATTTCCTTTTTTGTTCTCATTTACTAGTCTGATTGGCCAGATGTTTCCATGAACATTTCAGGATATTTCAACAATCAGGAAGCCACCAGCAAAACCATAGACGATCAGGGATGGGTACATACTGGAGATATAGGGTATTTCAATGAAGAAGGAGAATTGTTTGTTGTGGACAGAATCAAAGAACTTATCAAGTGTTATGGTTTTCAGGTTCCGCTTTTCATAATCAAATCCGTAACTTTCCATTTGGGAGTTCTTTATTTCCTTTTATTAGGCGCCTCTTACTAGCTCAAAGCATTTTATTAATTTATTAGTGCTTCATCAGGCTGTAATCAGTTCTTCTTCTGTTACTTACCGAGTTGTCTAGAAAATTACCGTCATTAGGATCATGGGTGGCACAGAAACTTGTCTATGTGTAAACGTTGTTTTTTCTTTTCTGTTTTGCATGATTACTAATCAGTAACTACAAATATTAACGTATGCATCCTCTTTTTAATGTTTTGTTGATAGTTTATGATCGGTTGTTATATATATATGTATATATATATATATATATTTTTTTTTTTTTGCTGTTTCTCCCACAACACCTAGACGCAAGATAATGCACCTCTGATGATGCAACAAGGATTACTTTTCACATTAATCTAGAATCTCTTGTATGCTAAGTTCTTCCAAGTTTAACAATATGATTCTTAGATGTACTGATTCTTTGCTTGGCTTCGTCGAATATGTTTAATGTTGTATGTGTATTGACTCCTCTTCCTGGAATTATGAACTCTAACTGCATTTATTAGTTGAATGGTATCAAATTTTTTTAACTTTCTTGTTTGCATATTAGGTTGCACCGGCAGAGCTTGAAGCATTGCTACTTTCTCACCCTCAAATAACAGATGCCATTGTGATCCCGTAAGTGTGTCGTACTTATTTCTGTTTTCTTACTTTCTTGTTAACCTCCAACGTTACTATCCACCTGCAGATATCCTGATGATAAAGCTGGTGAGGTCCCTATCGCATTTGTTGTTCGTTCACCCCATAGCTCGATAAAGGAAGAAGATGTTAAAAATTTTATTGCTGGGCAGGTAATATTTTGTAAACTTTTTGTTGATATGGATCTATTTTTACTCTTGAAAGCACCATCTTGTAATTCAGATGTCATTGGTGTCATATAATCTTTACACAGAGCACATTTTTCTTATTAAGCTCACTTGAAAGTTCGAACACGTGACCTCTAGGTACATGTTATTAGAGACTGTGTGTGTGCCCCTTTGCCAAGGGAGCTGCCTTTTAGGGGTATGTTTAAGATGCTTTTGTGTGCTGCATAATCCCAAAAATGGCAAAACAAGCAGGTGTAAAACAACCATCATTTTGACAATGGAATGCTTGAAAATGATCGCCCTCCTTTCCCTTGCAGGTTGCACCATATAAGAGATTAAAAAGAGTAACCTTCACAAGTAGTGTTCCGAAGTCGGTTTCGGGAAAACTTCTAAGAAGGGAGGTCATAGCACAAGTCCGTGCCAAGATGTAAATGTCAAAATGTTGACGGTAAGGTGAATAAGTTATACTGTTACACAGTTAGCAAGCAAATGTATTTATATTTGCAAGCAAAAGTAAACTGGAAATTTGTTGGAAATCTTAATACAACATTCATTCTTATGCTTAATAAAATCATGTTTGTTACCAATA

mRNA sequence

CAAAAAGTCAAATTTCAGTTTCATGTACTGTGGATAAAACCAAATCTTTCAACCCCCAAATTCCAAACCATCGTTTTTCTTCAATCCCATTTTAGCAATGGCGATCACGCTGCGCAAATCTTTCAATTCCCAATCGCAGATTTACACTTCCCGTCGTCCTCCAATCCACTTTCCGACCGACCCCACAATCTCCATTCCCTCATTCCTCTTTCGAAACTCCTCCTCTTTCCCTAACGCCCTCGCCCTCGCCGACTCCGACTCCGGCGAATCCTTAACTTTCCGTCAGCTTCAAATACAAGTCTCCAAGCTCGCCCATGTCTTCATCCAACTCGGGATTCGAAAAAGCGATGTGGTTCTCATATTCGCCCCTAATTCCATTCACTTCCCTGTTTGTTTCTTCGCCATTGTCGCCATTGGAGCCATCGCCACCACTTGTAATCCTGCTTACACTTTTGGGGAACTGTCAAAGCAAGTCGCTAACTGCAACCCCAAGCTCGTAATCACCGTTCCCGAACTCTGGGACACAATCGGTAAACTCAACTTGCCTTCTATAATTCTGGGATCTAAAATTTCTTCAAAATTTTCCCGTCCCAACATCTGGGGTTACTCCGATTTGATTAAAAAAGCCGGCGATGTGTCCAATTTGCCGGTGAGTGAAGTGGGTCAGAACGATGTAGCGGCGCTTTTATATTCATCTGGGACGACTGGGATTAGTAAGGGAGTGATTCTAACTCACAGGAACTTCATAACTACCTCTTTAATGGTGACCCAAGATCAGGATCTTCTTGGTGATCCTAGAAACGTGTTTTTATGCTTTCTTCCAATGTTCCATGTGTTTGGTCTCTCTATAATTGTTTACTCGCAGCTTCAAAGAGGAAACACTGTCGTTTCAATGGCGAAGTTTGAGCTAGAAAAGGCTTTGGGGGTGGTGATGAAGTACAGGATCACTCATCTTTATGTTGTTCCACCTGTAATTATAGCTCTCACAAAGCAAAGTGTAGTGAAGAACTACGACTTGTCGTCGTTGAAGCAGATTTTATCTGGGGCAGCGCCTCTTGGGAAGGATGTGATGGAAGAATGCTCCAAGTTTATACCTCAGGCCAGGATCATCCAGGGATATGGCATGACAGAAACTTGTGGAGTTATTTCAGTGGGGAATGTGGGAGTAGAATCTACGCTATCTGGGGCAACTGGATCCCTTGTTTCAGGAGTTGAAGCGCAAGTTTTGAGTACAGAAACACAGAAACGTCTTCCACCCGGTGAAACAGGGGAAATTTGTGTGCGAGGACCAAATATGATGAAAGGATATTTCAACAATCAGGAAGCCACCAGCAAAACCATAGACGATCAGGGATGGGTACATACTGGAGATATAGGGTATTTCAATGAAGAAGGAGAATTGTTTGTTGTGGACAGAATCAAAGAACTTATCAAGTGTTATGGTTTTCAGGTTGCACCGGCAGAGCTTGAAGCATTGCTACTTTCTCACCCTCAAATAACAGATGCCATTGTGATCCCATATCCTGATGATAAAGCTGGTGAGGTCCCTATCGCATTTGTTGTTCGTTCACCCCATAGCTCGATAAAGGAAGAAGATGTTAAAAATTTTATTGCTGGGCAGGTTGCACCATATAAGAGATTAAAAAGAGTAACCTTCACAAGTAGTGTTCCGAAGTCGGTTTCGGGAAAACTTCTAAGAAGGGAGGTCATAGCACAAGTCCGTGCCAAGATGTAAATGTCAAAATGTTGACGGTAAGGTGAATAAGTTATACTGTTACACAGTTAGCAAGCAAATGTATTTATATTTGCAAGCAAAAGTAAACTGGAAATTTGTTGGAAATCTTAATACAACATTCATTCTTATGCTTAATAAAATCATGTTTGTTACCAATA

Coding sequence (CDS)

ATGGCGATCACGCTGCGCAAATCTTTCAATTCCCAATCGCAGATTTACACTTCCCGTCGTCCTCCAATCCACTTTCCGACCGACCCCACAATCTCCATTCCCTCATTCCTCTTTCGAAACTCCTCCTCTTTCCCTAACGCCCTCGCCCTCGCCGACTCCGACTCCGGCGAATCCTTAACTTTCCGTCAGCTTCAAATACAAGTCTCCAAGCTCGCCCATGTCTTCATCCAACTCGGGATTCGAAAAAGCGATGTGGTTCTCATATTCGCCCCTAATTCCATTCACTTCCCTGTTTGTTTCTTCGCCATTGTCGCCATTGGAGCCATCGCCACCACTTGTAATCCTGCTTACACTTTTGGGGAACTGTCAAAGCAAGTCGCTAACTGCAACCCCAAGCTCGTAATCACCGTTCCCGAACTCTGGGACACAATCGGTAAACTCAACTTGCCTTCTATAATTCTGGGATCTAAAATTTCTTCAAAATTTTCCCGTCCCAACATCTGGGGTTACTCCGATTTGATTAAAAAAGCCGGCGATGTGTCCAATTTGCCGGTGAGTGAAGTGGGTCAGAACGATGTAGCGGCGCTTTTATATTCATCTGGGACGACTGGGATTAGTAAGGGAGTGATTCTAACTCACAGGAACTTCATAACTACCTCTTTAATGGTGACCCAAGATCAGGATCTTCTTGGTGATCCTAGAAACGTGTTTTTATGCTTTCTTCCAATGTTCCATGTGTTTGGTCTCTCTATAATTGTTTACTCGCAGCTTCAAAGAGGAAACACTGTCGTTTCAATGGCGAAGTTTGAGCTAGAAAAGGCTTTGGGGGTGGTGATGAAGTACAGGATCACTCATCTTTATGTTGTTCCACCTGTAATTATAGCTCTCACAAAGCAAAGTGTAGTGAAGAACTACGACTTGTCGTCGTTGAAGCAGATTTTATCTGGGGCAGCGCCTCTTGGGAAGGATGTGATGGAAGAATGCTCCAAGTTTATACCTCAGGCCAGGATCATCCAGGGATATGGCATGACAGAAACTTGTGGAGTTATTTCAGTGGGGAATGTGGGAGTAGAATCTACGCTATCTGGGGCAACTGGATCCCTTGTTTCAGGAGTTGAAGCGCAAGTTTTGAGTACAGAAACACAGAAACGTCTTCCACCCGGTGAAACAGGGGAAATTTGTGTGCGAGGACCAAATATGATGAAAGGATATTTCAACAATCAGGAAGCCACCAGCAAAACCATAGACGATCAGGGATGGGTACATACTGGAGATATAGGGTATTTCAATGAAGAAGGAGAATTGTTTGTTGTGGACAGAATCAAAGAACTTATCAAGTGTTATGGTTTTCAGGTTGCACCGGCAGAGCTTGAAGCATTGCTACTTTCTCACCCTCAAATAACAGATGCCATTGTGATCCCATATCCTGATGATAAAGCTGGTGAGGTCCCTATCGCATTTGTTGTTCGTTCACCCCATAGCTCGATAAAGGAAGAAGATGTTAAAAATTTTATTGCTGGGCAGGTTGCACCATATAAGAGATTAAAAAGAGTAACCTTCACAAGTAGTGTTCCGAAGTCGGTTTCGGGAAAACTTCTAAGAAGGGAGGTCATAGCACAAGTCCGTGCCAAGATGTAA

Protein sequence

MAITLRKSFNSQSQIYTSRRPPIHFPTDPTISIPSFLFRNSSSFPNALALADSDSGESLTFRQLQIQVSKLAHVFIQLGIRKSDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFGELSKQVANCNPKLVITVPELWDTIGKLNLPSIILGSKISSKFSRPNIWGYSDLIKKAGDVSNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQDLLGDPRNVFLCFLPMFHVFGLSIIVYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYVVPPVIIALTKQSVVKNYDLSSLKQILSGAAPLGKDVMEECSKFIPQARIIQGYGMTETCGVISVGNVGVESTLSGATGSLVSGVEAQVLSTETQKRLPPGETGEICVRGPNMMKGYFNNQEATSKTIDDQGWVHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPYPDDKAGEVPIAFVVRSPHSSIKEEDVKNFIAGQVAPYKRLKRVTFTSSVPKSVSGKLLRREVIAQVRAKM
Homology
BLAST of CmUC10G201130 vs. NCBI nr
Match: XP_038905825.1 (4-coumarate--CoA ligase-like 7 isoform X1 [Benincasa hispida])

HSP 1 Score: 1001.9 bits (2589), Expect = 2.1e-288
Identity = 505/545 (92.66%), Postives = 526/545 (96.51%), Query Frame = 0

Query: 1   MAITLRKSFNSQSQIYTSRRPPIHFPTDPTISIPSFLFRNSSSFPNALALADSDSGESLT 60
           MAIT+ KSFN QSQIYTSRRPPIHFPTDPTISI SFLFRNSS++PNALALAD+DSGESLT
Sbjct: 1   MAITMNKSFNPQSQIYTSRRPPIHFPTDPTISIASFLFRNSSNYPNALALADADSGESLT 60

Query: 61  FRQLQIQVSKLAHVFIQLGIRKSDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFG 120
           FRQ +IQVSKLAHV+IQLGIRK DVVLIFAPNSIHFPVCFFAIVAIGAI TTCNPAYTF 
Sbjct: 61  FRQFKIQVSKLAHVYIQLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAITTTCNPAYTFA 120

Query: 121 ELSKQVANCNPKLVITVPELWDTIGKLNLPSIILGSKISSKFSRPNIWGYSDLIKKAGDV 180
           ELSKQVANCNPKLVIT+PELWD +GKLNLPSIILGSKISSKFSRPNIWGYSDLIKKAGDV
Sbjct: 121 ELSKQVANCNPKLVITIPELWDVVGKLNLPSIILGSKISSKFSRPNIWGYSDLIKKAGDV 180

Query: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQDLLGDPRNVFLCF 240
           SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFI TSLMVTQDQDLLGDPRNVFLCF
Sbjct: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFIATSLMVTQDQDLLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSIIVYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYVVPPVIIALTKQS 300
           LPMFHVFGLSI+VY+QLQRGNTVVSMAKFELEKALG+VMKYRITHLYVVPPVIIAL KQS
Sbjct: 241 LPMFHVFGLSIVVYAQLQRGNTVVSMAKFELEKALGLVMKYRITHLYVVPPVIIALAKQS 300

Query: 301 VVKNYDLSSLKQILSGAAPLGKDVMEECSKFIPQARIIQGYGMTETCGVISVGNVGVEST 360
           VVKNYDLSSL+QILSGAAPLGKDVMEECSK IP ARIIQGYGMTETCGVISV NVG+EST
Sbjct: 301 VVKNYDLSSLRQILSGAAPLGKDVMEECSKIIPHARIIQGYGMTETCGVISVENVGIEST 360

Query: 361 LSGATGSLVSGVEAQVLSTETQKRLPPGETGEICVRGPNMMKGYFNNQEATSKTIDDQGW 420
           LSGATGSLVSGVEA++LSTETQKRLPPGETGEICVRGPNMMKGYFNNQ+ATS+TIDDQGW
Sbjct: 361 LSGATGSLVSGVEAEILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420

Query: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPYPDDKA 480
           VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPYPDDKA
Sbjct: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPYPDDKA 480

Query: 481 GEVPIAFVVRSPHSSIKEEDVKNFIAGQVAPYKRLKRVTFTSSVPKSVSGKLLRREVIAQ 540
           GEVPIAFVV SP S+IKEEDVK FIA QVAP+KRL+RVTFT SVPKS SGKLLRREV+AQ
Sbjct: 481 GEVPIAFVVCSPDSTIKEEDVKKFIARQVAPFKRLRRVTFTRSVPKSASGKLLRREVVAQ 540

Query: 541 VRAKM 546
           VRAKM
Sbjct: 541 VRAKM 545

BLAST of CmUC10G201130 vs. NCBI nr
Match: XP_011651966.2 (4-coumarate--CoA ligase-like 7 [Cucumis sativus] >KAE8651043.1 hypothetical protein Csa_001162 [Cucumis sativus])

HSP 1 Score: 985.7 bits (2547), Expect = 1.6e-283
Identity = 502/545 (92.11%), Postives = 522/545 (95.78%), Query Frame = 0

Query: 1   MAITLRKSFNSQSQIYTSRRPPIHFPTDPTISIPSFLFRNSSSFPNALALADSDSGESLT 60
           M IT+ KSFN QSQ+YTSRRPPIHFPTDPTISI SFLFRNSSS+PNALAL D+DSGESLT
Sbjct: 1   MDITMNKSFNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLT 60

Query: 61  FRQLQIQVSKLAHVFIQLGIRKSDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFG 120
           FRQLQIQVSKLAHVFIQLGI+K DVVLIFAPNSIHF VCFFAIVAIGAIATTCNPAYT  
Sbjct: 61  FRQLQIQVSKLAHVFIQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTSA 120

Query: 121 ELSKQVANCNPKLVITVPELWDTIGKLNLPSIILGSKISSKFSRPNIWGYSDLIKKAGDV 180
           ELSKQVANC PKLVITVPELWD IGKLNLPSIILGSKISSKFSR NIW YSDLIKKAGDV
Sbjct: 121 ELSKQVANCKPKLVITVPELWDVIGKLNLPSIILGSKISSKFSRSNIWAYSDLIKKAGDV 180

Query: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQDLLGDPRNVFLCF 240
           SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFIT SLMVTQDQ+LLGDPRNVFLCF
Sbjct: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSIIVYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYVVPPVIIALTKQS 300
           LPMFHVFGLSI+V SQLQRGNTVVSMAKFELEKALG+VMKY+ITHLYVVPPVIIALTKQ 
Sbjct: 241 LPMFHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK 300

Query: 301 VVKNYDLSSLKQILSGAAPLGKDVMEECSKFIPQARIIQGYGMTETCGVISVGNVGVEST 360
           VVKNYDLSSL+QILSGAAPLGKDVM+ECSK IPQARIIQGYGMTETCGVISV NVGVEST
Sbjct: 301 VVKNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVEST 360

Query: 361 LSGATGSLVSGVEAQVLSTETQKRLPPGETGEICVRGPNMMKGYFNNQEATSKTIDDQGW 420
            SGATGSLVSGVEAQ+LS ETQKRLPPGETGEICVRGPNMMKGYFNNQ+ATS+TIDDQGW
Sbjct: 361 QSGATGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420

Query: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPYPDDKA 480
           VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIP+PDDKA
Sbjct: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKA 480

Query: 481 GEVPIAFVVRSPHSSIKEEDVKNFIAGQVAPYKRLKRVTFTSSVPKSVSGKLLRREVIAQ 540
           GEVPIAFVVRSP+SSI EEDVK F+AGQVAP+KRL++VTFTSSVPKS SGKLLRREVIAQ
Sbjct: 481 GEVPIAFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQ 540

Query: 541 VRAKM 546
           VRAKM
Sbjct: 541 VRAKM 545

BLAST of CmUC10G201130 vs. NCBI nr
Match: XP_008442710.1 (PREDICTED: 4-coumarate--CoA ligase-like 7 [Cucumis melo] >KAA0043992.1 4-coumarate--CoA ligase-like 7 [Cucumis melo var. makuwa] >TYK25148.1 4-coumarate--CoA ligase-like 7 [Cucumis melo var. makuwa])

HSP 1 Score: 981.5 bits (2536), Expect = 3.0e-282
Identity = 500/545 (91.74%), Postives = 522/545 (95.78%), Query Frame = 0

Query: 1   MAITLRKSFNSQSQIYTSRRPPIHFPTDPTISIPSFLFRNSSSFPNALALADSDSGESLT 60
           M IT+ KSFN QS+IY+S RPPIHFPTDP ISI SFLFRNSSS+PNALALAD+DSGESLT
Sbjct: 1   MEITMNKSFNPQSRIYSSPRPPIHFPTDPKISIVSFLFRNSSSYPNALALADADSGESLT 60

Query: 61  FRQLQIQVSKLAHVFIQLGIRKSDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFG 120
           FRQLQIQVSKLA VF QLGI+K DVVLIFAPNSIHF VCFFAIVAIGAIATTCNPAYTF 
Sbjct: 61  FRQLQIQVSKLARVFSQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTFA 120

Query: 121 ELSKQVANCNPKLVITVPELWDTIGKLNLPSIILGSKISSKFSRPNIWGYSDLIKKAGDV 180
           ELSKQVANCNPKLVITVPELWD IGKLNLPS+ILGSKISSKFSR NIW YSDLIKKAGDV
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSMILGSKISSKFSRSNIWSYSDLIKKAGDV 180

Query: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQDLLGDPRNVFLCF 240
           SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQ+LLGDPRNVFLCF
Sbjct: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQELLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSIIVYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYVVPPVIIALTKQS 300
           LPMFHVFGLS+IVYSQLQRGNTVVSMAKFELEKALG+VMKY+ITHLYVVPPVIIALTKQ 
Sbjct: 241 LPMFHVFGLSVIVYSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK 300

Query: 301 VVKNYDLSSLKQILSGAAPLGKDVMEECSKFIPQARIIQGYGMTETCGVISVGNVGVEST 360
           VVKNYDLSSL+QILSGAAPLGKDVMEECSK IPQARIIQGYGMTETCGVISV N+ VEST
Sbjct: 301 VVKNYDLSSLRQILSGAAPLGKDVMEECSKIIPQARIIQGYGMTETCGVISVENMVVEST 360

Query: 361 LSGATGSLVSGVEAQVLSTETQKRLPPGETGEICVRGPNMMKGYFNNQEATSKTIDDQGW 420
           LSGATGSL SG+EAQ+LS ETQKRLPPGETGEICVRGPNMMKGYFNNQ+ATS+TIDDQGW
Sbjct: 361 LSGATGSLGSGIEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420

Query: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPYPDDKA 480
           VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIP+PDDKA
Sbjct: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKA 480

Query: 481 GEVPIAFVVRSPHSSIKEEDVKNFIAGQVAPYKRLKRVTFTSSVPKSVSGKLLRREVIAQ 540
           GEVPIAFVVRSP+SSI EEDVK FIAGQVAP+KRL++VTFTSSVPKS SGKLLRREVIAQ
Sbjct: 481 GEVPIAFVVRSPNSSISEEDVKKFIAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQ 540

Query: 541 VRAKM 546
           VRAKM
Sbjct: 541 VRAKM 545

BLAST of CmUC10G201130 vs. NCBI nr
Match: XP_022982901.1 (4-coumarate--CoA ligase-like 7 [Cucurbita maxima])

HSP 1 Score: 967.2 bits (2499), Expect = 5.8e-278
Identity = 489/545 (89.72%), Postives = 517/545 (94.86%), Query Frame = 0

Query: 1   MAITLRKSFNSQSQIYTSRRPPIHFPTDPTISIPSFLFRNSSSFPNALALADSDSGESLT 60
           MAI + KSFN QSQIYTS RPPIHFPTDPTISI SFLFRNSSSF ++LALAD+DSGESLT
Sbjct: 1   MAIAMAKSFNPQSQIYTSPRPPIHFPTDPTISILSFLFRNSSSFLHSLALADADSGESLT 60

Query: 61  FRQLQIQVSKLAHVFIQLGIRKSDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFG 120
           FRQLQIQVSKLAH FI LGIRK DVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTF 
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFA 120

Query: 121 ELSKQVANCNPKLVITVPELWDTIGKLNLPSIILGSKISSKFSRPNIWGYSDLIKKAGDV 180
           E+SKQVA+CNPKLVIT+PELWD IGKLNLPSIILGSKISSK SRPNIW YSDLIK +GDV
Sbjct: 121 EISKQVASCNPKLVITIPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV 180

Query: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQDLLGDPRNVFLCF 240
           S+LPVSEVGQ+DVAALLYSSGTTGI+KGVILTHRNFITTSLMVT DQDLLGDPRNVFLCF
Sbjct: 181 SDLPVSEVGQSDVAALLYSSGTTGINKGVILTHRNFITTSLMVTHDQDLLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSIIVYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYVVPPVIIALTKQS 300
           LPMFHVFGLS+I+YSQLQRGNTVVSMAKFELEKALGVVMKYRITHLY+VPPVIIA+ KQS
Sbjct: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300

Query: 301 VVKNYDLSSLKQILSGAAPLGKDVMEECSKFIPQARIIQGYGMTETCGVISVGNVGVEST 360
           VVK YDLSSLKQILSGAAPLGKDVMEECSK +PQ +I QGYGMTETCGVIS+ N+GVES 
Sbjct: 301 VVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESR 360

Query: 361 LSGATGSLVSGVEAQVLSTETQKRLPPGETGEICVRGPNMMKGYFNNQEATSKTIDDQGW 420
           LSG+TG LVSG+EAQ+LSTETQKRLPPGETGEICVRGPNMMKGYFNNQ+ATS+TIDDQGW
Sbjct: 361 LSGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420

Query: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPYPDDKA 480
           VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQI DAIVIPYPDDKA
Sbjct: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKA 480

Query: 481 GEVPIAFVVRSPHSSIKEEDVKNFIAGQVAPYKRLKRVTFTSSVPKSVSGKLLRREVIAQ 540
           GEVPIAFVVRSP SSI EEDVK+FIAGQVAP+KRLK VTFTSS+PKS SGKLLRRE+IAQ
Sbjct: 481 GEVPIAFVVRSPSSSINEEDVKSFIAGQVAPFKRLKTVTFTSSIPKSASGKLLRRELIAQ 540

Query: 541 VRAKM 546
           VRAKM
Sbjct: 541 VRAKM 545

BLAST of CmUC10G201130 vs. NCBI nr
Match: KAG6580619.1 (4-coumarate--CoA ligase-like 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 966.5 bits (2497), Expect = 9.9e-278
Identity = 491/545 (90.09%), Postives = 517/545 (94.86%), Query Frame = 0

Query: 1   MAITLRKSFNSQSQIYTSRRPPIHFPTDPTISIPSFLFRNSSSFPNALALADSDSGESLT 60
           MAI + K F+ QSQIYTS RPPIHFPTDPTISI SFLFRNSSSF ++LALAD+DSGESLT
Sbjct: 1   MAIAVAKYFDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLT 60

Query: 61  FRQLQIQVSKLAHVFIQLGIRKSDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFG 120
           FRQLQIQVSKLAH FI LGIRK DVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTF 
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120

Query: 121 ELSKQVANCNPKLVITVPELWDTIGKLNLPSIILGSKISSKFSRPNIWGYSDLIKKAGDV 180
           ELSKQVANCNPKLVITVPELWD IGKLNLPSIILGSKISSK SRPNIW YSDLIK +GDV
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV 180

Query: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQDLLGDPRNVFLCF 240
           S+LPVSEVGQ+DVAALLYSSGTTGISKGVILTHRNFI TSLMVT DQDLLGDPRNVFLCF
Sbjct: 181 SDLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSIIVYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYVVPPVIIALTKQS 300
           LPMFHVFGLS+I+YSQLQRGNTVVSMAKFELEKALGVVMKYRITHLY+VPPVIIA+ KQS
Sbjct: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300

Query: 301 VVKNYDLSSLKQILSGAAPLGKDVMEECSKFIPQARIIQGYGMTETCGVISVGNVGVEST 360
           VVK YDLSSLKQILSGAAPLGKDVMEECSK +PQ +I QGYGMTETCGVIS+ N+GVES 
Sbjct: 301 VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESR 360

Query: 361 LSGATGSLVSGVEAQVLSTETQKRLPPGETGEICVRGPNMMKGYFNNQEATSKTIDDQGW 420
           LSG+TG LVSG+EAQ+LSTETQKRLPPGETGEICVRGPNMMKGYFNNQ+ATS+TIDDQGW
Sbjct: 361 LSGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420

Query: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPYPDDKA 480
           VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQI DAIVIPYPDDKA
Sbjct: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKA 480

Query: 481 GEVPIAFVVRSPHSSIKEEDVKNFIAGQVAPYKRLKRVTFTSSVPKSVSGKLLRREVIAQ 540
           GEVPIAFVVRSP+SSI EEDVK+FIAGQVAP++RLKRVTFTSSVPKS SGKLLRRE+IAQ
Sbjct: 481 GEVPIAFVVRSPNSSINEEDVKSFIAGQVAPFRRLKRVTFTSSVPKSASGKLLRRELIAQ 540

Query: 541 VRAKM 546
           VRAKM
Sbjct: 541 VRAKM 545

BLAST of CmUC10G201130 vs. ExPASy Swiss-Prot
Match: M4IQS1 (Probable CoA ligase CCL10 OS=Humulus lupulus OX=3486 GN=CCL10 PE=2 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 1.3e-211
Identity = 365/543 (67.22%), Postives = 451/543 (83.06%), Query Frame = 0

Query: 5   LRKSFNSQSQIYTSRRPPIHFPTDPTISIPSFLFRNSSSFPNALALADSDSGESLTFRQL 64
           + K FN ++QIY+S RPP++FPTDP +S+ SFLFR+S+S+PN  AL D+DSG++LTF +L
Sbjct: 1   MEKCFNPETQIYSSHRPPVNFPTDPKLSLTSFLFRSSASYPNRTALIDADSGQTLTFLKL 60

Query: 65  QIQVSKLAHVFIQLGIRKSDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFGELSK 124
           + QVSKLAH  +QL I+K+DVVLIFAPNSIHFPVCFF+I A+GAI TTCNP+YTF ELS 
Sbjct: 61  KTQVSKLAHSLVQLNIKKNDVVLIFAPNSIHFPVCFFSIAALGAITTTCNPSYTFTELSN 120

Query: 125 QVANCNPKLVITVPELWDTIGKLNLPSIILGSKISSKF-SRPNIWGYSDLIKKAGDVSNL 184
           Q  +CNP LVITVPELW+   KLNLP+IIL S  +SK  S+   W +SDL +K+   S L
Sbjct: 121 QAKDCNPTLVITVPELWEKARKLNLPAIILPSPSNSKLSSKSEFWFFSDLTRKSDRFSEL 180

Query: 185 PVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQDLLGDPRNVFLCFLPM 244
           P+S+V Q+DVAALLYSSGTTG SKGV+L+H+NFITTSLMVT DQD  GDP+N+ +CFLPM
Sbjct: 181 PISDVRQSDVAALLYSSGTTGKSKGVVLSHKNFITTSLMVTSDQDRYGDPQNICMCFLPM 240

Query: 245 FHVFGLSIIVYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYVVPPVIIALTKQS-VV 304
           FH+FGLS+I YSQL+RGN VVSM KFELE AL  V  YR+THL+VVPPV+IAL K+S VV
Sbjct: 241 FHIFGLSVIAYSQLRRGNGVVSMGKFELEGALRAVEMYRVTHLFVVPPVMIALAKESVVV 300

Query: 305 KNYDLSSLKQILSGAAPLGKDVMEECSKFIPQARIIQGYGMTETCGVISVGNVGVESTLS 364
           + YDLSS+K+ILSGAAPLGK+VME+C++ +P A IIQGYGMTETCG+IS+ +       S
Sbjct: 301 RRYDLSSVKEILSGAAPLGKNVMEQCARNVPGAAIIQGYGMTETCGIISIEDSKKGCRFS 360

Query: 365 GATGSLVSGVEAQVLSTETQKRLPPGETGEICVRGPNMMKGYFNNQEATSKTIDDQGWVH 424
           G+TG L  G+E+Q++ T+    LPP + GEI +RGPNMM+GY NN EAT  TID+QGWV 
Sbjct: 361 GSTGMLAPGIESQIMDTKRWNPLPPNQEGEIWLRGPNMMQGYLNNLEATKSTIDEQGWVK 420

Query: 425 TGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPYPDDKAGE 484
           TGDIGYF+EEG+LFVVDR+KELIKCYGFQVAPAELEALLLSHP+I DA+VIP+PD+KAGE
Sbjct: 421 TGDIGYFDEEGQLFVVDRLKELIKCYGFQVAPAELEALLLSHPEILDAVVIPFPDEKAGE 480

Query: 485 VPIAFVVRSPHSSIKEEDVKNFIAGQVAPYKRLKRVTFTSSVPKSVSGKLLRREVIAQVR 544
           VPIA VVRSP+SS+ EEDV+ FI  QVAP+K+L+RVTF SSV KS +GK+LRRE+I +VR
Sbjct: 481 VPIANVVRSPNSSLSEEDVQRFIEKQVAPFKKLRRVTFVSSVLKSPAGKILRRELIQKVR 540

Query: 545 AKM 546
           +K+
Sbjct: 541 SKI 543

BLAST of CmUC10G201130 vs. ExPASy Swiss-Prot
Match: M4IRL6 (Probable CoA ligase CCL7 OS=Humulus lupulus OX=3486 GN=CCL7 PE=2 SV=1)

HSP 1 Score: 672.9 bits (1735), Expect = 3.0e-192
Identity = 338/544 (62.13%), Postives = 429/544 (78.86%), Query Frame = 0

Query: 5   LRKSFNSQSQIYTSRRPPIHFPTDPTISIPSFLFRNSSSFPNALALADSDSGESLTFRQL 64
           + KS   +  ++ S RPP+  P D  +S+ SF+FRNSSS+P   AL DSD+ E+L+F Q 
Sbjct: 1   MEKSGYGRDGVFRSLRPPLVLPKDHNLSMVSFVFRNSSSYPQKPALIDSDTNETLSFSQF 60

Query: 65  QIQVSKLAHVFIQLGIRKSDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFGELSK 124
           +  V K++H F+ LG++K+DVVLIFAPNSIH PVCF  IVA GAIATT NP YT  ELSK
Sbjct: 61  KSMVIKVSHGFLNLGVQKNDVVLIFAPNSIHVPVCFLGIVASGAIATTSNPLYTVSELSK 120

Query: 125 QVANCNPKLVITVPELWDTIGKLNLPSIILG--SKISSKF-SRPNIWGYSDLIKKAGDVS 184
           QV + NPKL++TVPEL++ +   NLP+I++G  S+ SS   SR  +  + DL+  +G VS
Sbjct: 121 QVKDSNPKLIVTVPELFEKVKGFNLPTILIGPNSEDSSPLKSRAKVLTFHDLVTLSGPVS 180

Query: 185 NLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQDLLGDPRNVFLCFL 244
           + P+ +  Q+D AALLYSSGTTG+SKGV+L+H+NFI +SLMVT +QD  G+  NVFLCFL
Sbjct: 181 DFPMVDFKQSDTAALLYSSGTTGMSKGVVLSHKNFIASSLMVTMEQDQAGEMHNVFLCFL 240

Query: 245 PMFHVFGLSIIVYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYVVPPVIIALTKQSV 304
           PMFHVFGL+II Y+QLQRGNTV+SMA+F+LEK L  V KY++THL+VVPPVI+ALTK S+
Sbjct: 241 PMFHVFGLAIITYAQLQRGNTVISMARFDLEKILKDVEKYKVTHLWVVPPVILALTKNSI 300

Query: 305 VKNYDLSSLKQILSGAAPLGKDVMEECSKFIPQARIIQGYGMTETCGVISVGNVGVESTL 364
           VK YDLSSLK I SGAAPLGKD+MEEC+K +P   + QGYGMTETCG++SV +       
Sbjct: 301 VKKYDLSSLKHIGSGAAPLGKDLMEECAKIVPHGIVAQGYGMTETCGIVSVEDTRGGKRH 360

Query: 365 SGATGSLVSGVEAQVLSTETQKRLPPGETGEICVRGPNMMKGYFNNQEATSKTIDDQGWV 424
           +G+ G L SGVEAQ++S +T K LPP + GEI VRGPNMM+GYFNN  AT  TID +GWV
Sbjct: 361 TGSAGMLSSGVEAQIVSVDTLKPLPPNQLGEIWVRGPNMMQGYFNNPRATKLTIDKKGWV 420

Query: 425 HTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPYPDDKAG 484
           HTGD+GYF+E+G L+VVDRIKELIK  GFQVAPAELE LL+SHP+I DA+VIP+PD  AG
Sbjct: 421 HTGDLGYFDEDGHLYVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDADAG 480

Query: 485 EVPIAFVVRSPHSSIKEEDVKNFIAGQVAPYKRLKRVTFTSSVPKSVSGKLLRREVIAQV 544
           EVP+A+VVRSP+SS+ E+DVK FIAGQVA +KRL++VTF +SVPKS SGK+LRRE+I +V
Sbjct: 481 EVPVAYVVRSPNSSLTEDDVKKFIAGQVASFKRLRKVTFINSVPKSASGKILRRELIQKV 540

Query: 545 RAKM 546
           R+ +
Sbjct: 541 RSNI 544

BLAST of CmUC10G201130 vs. ExPASy Swiss-Prot
Match: Q9M0X9 (4-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 1.3e-190
Identity = 337/544 (61.95%), Postives = 423/544 (77.76%), Query Frame = 0

Query: 5   LRKSFNSQSQIYTSRRPPIHFPTDPTISIPSFLFRNSSSFPNALALADSDSGESLTFRQL 64
           + KS   +  IY S RP +  P DP  S+ SFLFRNSSS+P+ LA+ADSD+G+SLTF QL
Sbjct: 1   MEKSGYGRDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQL 60

Query: 65  QIQVSKLAHVFIQLGIRKSDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFGELSK 124
           +  V++LAH F +LGIRK+DVVLIFAPNS  FP+CF A+ AIG + TT NP YT  E+SK
Sbjct: 61  KSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSK 120

Query: 125 QVANCNPKLVITVPELWDTIGKLNLPSIILGSKISSKF---SRPNIWGYSDLIKKAGDVS 184
           Q+ + NPK++I+V +L+D I   +LP ++LGSK + +    S   I  + ++++ +  VS
Sbjct: 121 QIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEPVS 180

Query: 185 NLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQDLLGDPRNVFLCFL 244
             P  E+ Q+D AALLYSSGTTG SKGV LTH NFI  SLMVT DQDL+G+   VFLCFL
Sbjct: 181 EYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLCFL 240

Query: 245 PMFHVFGLSIIVYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYVVPPVIIALTKQSV 304
           PMFHVFGL++I YSQLQRGN +VSMA+FELE  L  + K+R+THL+VVPPV +AL+KQS+
Sbjct: 241 PMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSI 300

Query: 305 VKNYDLSSLKQILSGAAPLGKDVMEECSKFIPQARIIQGYGMTETCGVISVGNVGVESTL 364
           VK +DLSSLK I SGAAPLGKD+MEEC + IP   ++QGYGMTETCG++SV +  +    
Sbjct: 301 VKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLGKRN 360

Query: 365 SGATGSLVSGVEAQVLSTETQKRLPPGETGEICVRGPNMMKGYFNNQEATSKTIDDQGWV 424
           SG+ G L  GVEAQ++S ET K  PP + GEI VRGPNMMKGY NN +AT +TID + WV
Sbjct: 361 SGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWV 420

Query: 425 HTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPYPDDKAG 484
           HTGD+GYFNE+G L+VVDRIKELIK  GFQVAPAELE LL+SHP I DA+VIP+PD++AG
Sbjct: 421 HTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAG 480

Query: 485 EVPIAFVVRSPHSSIKEEDVKNFIAGQVAPYKRLKRVTFTSSVPKSVSGKLLRREVIAQV 544
           EVPIAFVVRSP+SSI E+D++ FIA QVAPYKRL+RV+F S VPKS +GK+LRRE++ QV
Sbjct: 481 EVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQQV 540

Query: 545 RAKM 546
           R+KM
Sbjct: 541 RSKM 544

BLAST of CmUC10G201130 vs. ExPASy Swiss-Prot
Match: Q0DV32 (4-coumarate--CoA ligase-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL1 PE=2 SV=2)

HSP 1 Score: 556.2 bits (1432), Expect = 4.1e-157
Identity = 298/538 (55.39%), Postives = 385/538 (71.56%), Query Frame = 0

Query: 15  IYTSRRPPIHFPTDPTISIPSFLFRNSSSFPNALALADSDS-GESLTFRQLQIQV-SKLA 74
           +Y S RPP    +DP +S+   L R + + P+A+ALAD+ + G +LTF +L+  V S   
Sbjct: 16  VYRSLRPPAPVASDPGLSLTDLLLRRADACPSAVALADAAAGGRALTFAELRSAVLSTAV 75

Query: 75  HVFIQLGIRKSDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFGELSKQVANCNPK 134
            +  + G+R  D VL+ APN + +PVCFFA+ A+GA+ TT NP YT  E++KQV++   K
Sbjct: 76  ALSSRAGVRPGDAVLLLAPNCVLYPVCFFAVTALGAVGTTVNPDYTPREIAKQVSDARAK 135

Query: 135 LVITVPELWDTIGKLNLPSIILGSKISSKFSR----PNIWGYSDLIK--KAGDVSNLPVS 194
           LVIT+  L   I  L LP I+L    ++  +       +  Y++L+   K  D    P+ 
Sbjct: 136 LVITISALVPKIAGLRLPVILLDDDANAAAASLPPDATVTLYTNLVAGVKEADYRRPPIK 195

Query: 195 EVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQDLLGDPRNVFLCFLPMFHV 254
              Q+D AALLYSSGTTG SKGVILTHRNFI  + MVT DQD   +  NVFLCFLPMFH+
Sbjct: 196 ---QSDTAALLYSSGTTGDSKGVILTHRNFIAAARMVTSDQDERREGPNVFLCFLPMFHI 255

Query: 255 FGLSIIVYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYVVPPVIIALTKQSVVKNYD 314
           FGLS+I Y+QL RGN +++M++F++   +  V ++R+THL+ VPPVIIAL K      YD
Sbjct: 256 FGLSVITYAQLHRGNAIIAMSRFDINSLMEAVQRHRVTHLFCVPPVIIALAKHGKAGKYD 315

Query: 315 LSSLKQILSGAAPLGKDVMEECSKFIPQARIIQGYGMTETCGVISVGNVGV-ESTLSGAT 374
           LSSLK I SGAAPLGKDVME  +K  P + I+QGYGMTETCG+IS+      ++   G+T
Sbjct: 316 LSSLKFIGSGAAPLGKDVMEVVAKKFPDSEIVQGYGMTETCGIISLEYPEKGQAREFGST 375

Query: 375 GSLVSGVEAQVLSTETQKRLPPGETGEICVRGPNMMKGYFNNQEATSKTIDDQGWVHTGD 434
           G+LVSGVEA+++  +T K LPP + GEICVRGPN+M+GYFNN +AT  TI  QGW+HTGD
Sbjct: 376 GTLVSGVEAKIVDIKTLKHLPPNQVGEICVRGPNVMQGYFNNVQATEFTI-KQGWLHTGD 435

Query: 435 IGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPYPDDKAGEVPI 494
           +GYF+  G+LFVVDR+KELIK  GFQ+APAELE LLLSHP+I DA+VIP+PD KAGEVPI
Sbjct: 436 LGYFDGGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPDAKAGEVPI 495

Query: 495 AFVVRSPHSSIKEEDVKNFIAGQVAPYKRLKRVTFTSSVPKSVSGKLLRREVIAQVRA 544
           A+VVRSP SS+ E DV+ FI  QVA YKRLKRVTF  SVPKS SGK+LRR++IAQVR+
Sbjct: 496 AYVVRSPDSSLTEVDVQKFIEKQVAYYKRLKRVTFVGSVPKSASGKILRRQLIAQVRS 549

BLAST of CmUC10G201130 vs. ExPASy Swiss-Prot
Match: M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 2.5e-122
Identity = 236/545 (43.30%), Postives = 349/545 (64.04%), Query Frame = 0

Query: 6   RKSFNSQSQIYTSRRPPIHFPTDPTISIPSFLFRNSSSFPNALALADSDSGESLTFRQLQ 65
           R  +   + I+ S+R P+  P + ++ + +F+  +S +    +A  D+ +G  LTF QL 
Sbjct: 12  RSGYCKSNSIFYSKREPVQLPQNHSVDVTTFI--SSRAHHGKIAFIDAATGRHLTFPQLW 71

Query: 66  IQVSKLAHVFIQLGIRKSDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFGELSKQ 125
             V  +A     +GIRK DV+L+ +PNSI+FPV   A++++GAI TT NP  T  E++KQ
Sbjct: 72  RAVDSVATCLSAMGIRKGDVILLLSPNSIYFPVVCLAVMSLGAIITTTNPLNTPREIAKQ 131

Query: 126 VANCNPKLVITVPELWDTIGKLNLPSIILGSKISSKFSRP-NIWGYSDLIKKAGDVSNLP 185
           + +  P L  T+P+L   I   NLP +I+  ++ S   +  NI      + +     N  
Sbjct: 132 ITDSKPVLAFTIPQLVSKIAGSNLPIVIIDDEVKSSLEKTLNIVSSLGEMMRKEPSPNRI 191

Query: 186 VSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQDLLG--DPRNVFLCFLP 245
              V Q D A LLYSSGTTG SKGV+ +H+N I    MV       G  D  + F+C +P
Sbjct: 192 GYRVNQEDTATLLYSSGTTGASKGVVSSHKNLIA---MVQTILSRFGTEDGEHTFICTVP 251

Query: 246 MFHVFGLSIIVYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYVVPPVIIALTKQS-- 305
           MFH++GL+      L  G+T+V ++KFE+ + L  + KYR T+L +VPP+++AL K +  
Sbjct: 252 MFHIYGLAAFAMGLLSSGSTIVILSKFEIHEMLSAIEKYRATYLPLVPPILMALLKNANH 311

Query: 306 VVKNYDLSSLKQILSGAAPLGKDVMEECSKFIPQARIIQGYGMTETCGVISVGNVGVEST 365
           +   YDLSSL+ +LSG APL K+V+E   +  P   I+QGYG+TE+ G+ +  +   ES 
Sbjct: 312 IRAKYDLSSLQSVLSGGAPLSKEVIEGFVENYPTVSILQGYGLTESTGIGASTDCLQESR 371

Query: 366 LSGATGSLVSGVEAQVLSTETQKRLPPGETGEICVRGPNMMKGYFNNQEATSKTIDDQGW 425
             G  G L   +EA++++ ET + L    TGE+ +RGP +MKGYF+N+EATS TID +GW
Sbjct: 372 RYGTAGMLSPSMEAKIVNPETGEALSVNRTGELWLRGPTIMKGYFSNEEATSSTIDSEGW 431

Query: 426 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPYPDDKA 485
           + TGD+ Y +E+G +FVVDR+KELIK  G+QVAPAELEALLLSHP+I+DA VIPYPD +A
Sbjct: 432 LRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLSHPEISDAAVIPYPDKEA 491

Query: 486 GEVPIAFVVRSPHSSIKEEDVKNFIAGQVAPYKRLKRVTFTSSVPKSVSGKLLRREVIAQ 545
           G+ P+A+VVR   S++ E  V +FIA  VAPYKR+++V F +S+PK+ SGK+LR+++I  
Sbjct: 492 GQFPMAYVVRKGGSNLSESTVMDFIAKLVAPYKRIRKVAFVASIPKNPSGKILRKDLIKL 551

BLAST of CmUC10G201130 vs. ExPASy TrEMBL
Match: A0A0A0LDK0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G740270 PE=4 SV=1)

HSP 1 Score: 986.9 bits (2550), Expect = 3.4e-284
Identity = 502/545 (92.11%), Postives = 523/545 (95.96%), Query Frame = 0

Query: 1   MAITLRKSFNSQSQIYTSRRPPIHFPTDPTISIPSFLFRNSSSFPNALALADSDSGESLT 60
           M IT+ KSFN QSQ+YTSRRPPIHFPTDPTISI SFLFRNSSS+PNALAL D+DSGESLT
Sbjct: 1   MDITMNKSFNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLT 60

Query: 61  FRQLQIQVSKLAHVFIQLGIRKSDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFG 120
           FRQLQIQVSKLAHVFIQLGI+K DVVLIF+PNSIHF VCFFAIVAIGAIATTCNPAYT  
Sbjct: 61  FRQLQIQVSKLAHVFIQLGIQKGDVVLIFSPNSIHFLVCFFAIVAIGAIATTCNPAYTSA 120

Query: 121 ELSKQVANCNPKLVITVPELWDTIGKLNLPSIILGSKISSKFSRPNIWGYSDLIKKAGDV 180
           ELSKQVANC PKLVITVPELWD IGKLNLPSIILGSKISSKFSR NIWGYSDLIKKAGDV
Sbjct: 121 ELSKQVANCKPKLVITVPELWDVIGKLNLPSIILGSKISSKFSRSNIWGYSDLIKKAGDV 180

Query: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQDLLGDPRNVFLCF 240
           SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFIT SLMVTQDQ+LLGDPRNVFLCF
Sbjct: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSIIVYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYVVPPVIIALTKQS 300
           LPMFHVFGLSI+V SQLQRGNTVVSMAKFELEKALG+VMKY+ITHLYVVPPVIIALTKQ 
Sbjct: 241 LPMFHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK 300

Query: 301 VVKNYDLSSLKQILSGAAPLGKDVMEECSKFIPQARIIQGYGMTETCGVISVGNVGVEST 360
           VVKNYDLSSL+QILSGAAPLGKDVM+ECSK IPQARIIQGYGMTETCGVISV NVGVEST
Sbjct: 301 VVKNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVEST 360

Query: 361 LSGATGSLVSGVEAQVLSTETQKRLPPGETGEICVRGPNMMKGYFNNQEATSKTIDDQGW 420
            SGATGSLVSGVEAQ+LS ETQKRLPPGETGEICVRGPNMMKGYFNNQ+ATS+TIDDQGW
Sbjct: 361 QSGATGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420

Query: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPYPDDKA 480
           VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIP+PDDKA
Sbjct: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKA 480

Query: 481 GEVPIAFVVRSPHSSIKEEDVKNFIAGQVAPYKRLKRVTFTSSVPKSVSGKLLRREVIAQ 540
           GEVPIAFVVRSP+SSI EEDVK F+AGQVAP+KRL++VTFTSSVPKS SGKLLRREVIAQ
Sbjct: 481 GEVPIAFVVRSPNSSISEEDVKIFVAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQ 540

Query: 541 VRAKM 546
           VRAKM
Sbjct: 541 VRAKM 545

BLAST of CmUC10G201130 vs. ExPASy TrEMBL
Match: A0A5A7TKZ4 (4-coumarate--CoA ligase-like 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002730 PE=4 SV=1)

HSP 1 Score: 981.5 bits (2536), Expect = 1.4e-282
Identity = 500/545 (91.74%), Postives = 522/545 (95.78%), Query Frame = 0

Query: 1   MAITLRKSFNSQSQIYTSRRPPIHFPTDPTISIPSFLFRNSSSFPNALALADSDSGESLT 60
           M IT+ KSFN QS+IY+S RPPIHFPTDP ISI SFLFRNSSS+PNALALAD+DSGESLT
Sbjct: 1   MEITMNKSFNPQSRIYSSPRPPIHFPTDPKISIVSFLFRNSSSYPNALALADADSGESLT 60

Query: 61  FRQLQIQVSKLAHVFIQLGIRKSDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFG 120
           FRQLQIQVSKLA VF QLGI+K DVVLIFAPNSIHF VCFFAIVAIGAIATTCNPAYTF 
Sbjct: 61  FRQLQIQVSKLARVFSQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTFA 120

Query: 121 ELSKQVANCNPKLVITVPELWDTIGKLNLPSIILGSKISSKFSRPNIWGYSDLIKKAGDV 180
           ELSKQVANCNPKLVITVPELWD IGKLNLPS+ILGSKISSKFSR NIW YSDLIKKAGDV
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSMILGSKISSKFSRSNIWSYSDLIKKAGDV 180

Query: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQDLLGDPRNVFLCF 240
           SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQ+LLGDPRNVFLCF
Sbjct: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQELLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSIIVYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYVVPPVIIALTKQS 300
           LPMFHVFGLS+IVYSQLQRGNTVVSMAKFELEKALG+VMKY+ITHLYVVPPVIIALTKQ 
Sbjct: 241 LPMFHVFGLSVIVYSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK 300

Query: 301 VVKNYDLSSLKQILSGAAPLGKDVMEECSKFIPQARIIQGYGMTETCGVISVGNVGVEST 360
           VVKNYDLSSL+QILSGAAPLGKDVMEECSK IPQARIIQGYGMTETCGVISV N+ VEST
Sbjct: 301 VVKNYDLSSLRQILSGAAPLGKDVMEECSKIIPQARIIQGYGMTETCGVISVENMVVEST 360

Query: 361 LSGATGSLVSGVEAQVLSTETQKRLPPGETGEICVRGPNMMKGYFNNQEATSKTIDDQGW 420
           LSGATGSL SG+EAQ+LS ETQKRLPPGETGEICVRGPNMMKGYFNNQ+ATS+TIDDQGW
Sbjct: 361 LSGATGSLGSGIEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420

Query: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPYPDDKA 480
           VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIP+PDDKA
Sbjct: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKA 480

Query: 481 GEVPIAFVVRSPHSSIKEEDVKNFIAGQVAPYKRLKRVTFTSSVPKSVSGKLLRREVIAQ 540
           GEVPIAFVVRSP+SSI EEDVK FIAGQVAP+KRL++VTFTSSVPKS SGKLLRREVIAQ
Sbjct: 481 GEVPIAFVVRSPNSSISEEDVKKFIAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQ 540

Query: 541 VRAKM 546
           VRAKM
Sbjct: 541 VRAKM 545

BLAST of CmUC10G201130 vs. ExPASy TrEMBL
Match: A0A1S3B5U8 (4-coumarate--CoA ligase-like 7 OS=Cucumis melo OX=3656 GN=LOC103486502 PE=4 SV=1)

HSP 1 Score: 981.5 bits (2536), Expect = 1.4e-282
Identity = 500/545 (91.74%), Postives = 522/545 (95.78%), Query Frame = 0

Query: 1   MAITLRKSFNSQSQIYTSRRPPIHFPTDPTISIPSFLFRNSSSFPNALALADSDSGESLT 60
           M IT+ KSFN QS+IY+S RPPIHFPTDP ISI SFLFRNSSS+PNALALAD+DSGESLT
Sbjct: 1   MEITMNKSFNPQSRIYSSPRPPIHFPTDPKISIVSFLFRNSSSYPNALALADADSGESLT 60

Query: 61  FRQLQIQVSKLAHVFIQLGIRKSDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFG 120
           FRQLQIQVSKLA VF QLGI+K DVVLIFAPNSIHF VCFFAIVAIGAIATTCNPAYTF 
Sbjct: 61  FRQLQIQVSKLARVFSQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTFA 120

Query: 121 ELSKQVANCNPKLVITVPELWDTIGKLNLPSIILGSKISSKFSRPNIWGYSDLIKKAGDV 180
           ELSKQVANCNPKLVITVPELWD IGKLNLPS+ILGSKISSKFSR NIW YSDLIKKAGDV
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSMILGSKISSKFSRSNIWSYSDLIKKAGDV 180

Query: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQDLLGDPRNVFLCF 240
           SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQ+LLGDPRNVFLCF
Sbjct: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQELLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSIIVYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYVVPPVIIALTKQS 300
           LPMFHVFGLS+IVYSQLQRGNTVVSMAKFELEKALG+VMKY+ITHLYVVPPVIIALTKQ 
Sbjct: 241 LPMFHVFGLSVIVYSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQK 300

Query: 301 VVKNYDLSSLKQILSGAAPLGKDVMEECSKFIPQARIIQGYGMTETCGVISVGNVGVEST 360
           VVKNYDLSSL+QILSGAAPLGKDVMEECSK IPQARIIQGYGMTETCGVISV N+ VEST
Sbjct: 301 VVKNYDLSSLRQILSGAAPLGKDVMEECSKIIPQARIIQGYGMTETCGVISVENMVVEST 360

Query: 361 LSGATGSLVSGVEAQVLSTETQKRLPPGETGEICVRGPNMMKGYFNNQEATSKTIDDQGW 420
           LSGATGSL SG+EAQ+LS ETQKRLPPGETGEICVRGPNMMKGYFNNQ+ATS+TIDDQGW
Sbjct: 361 LSGATGSLGSGIEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420

Query: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPYPDDKA 480
           VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIP+PDDKA
Sbjct: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPHPDDKA 480

Query: 481 GEVPIAFVVRSPHSSIKEEDVKNFIAGQVAPYKRLKRVTFTSSVPKSVSGKLLRREVIAQ 540
           GEVPIAFVVRSP+SSI EEDVK FIAGQVAP+KRL++VTFTSSVPKS SGKLLRREVIAQ
Sbjct: 481 GEVPIAFVVRSPNSSISEEDVKKFIAGQVAPFKRLRKVTFTSSVPKSASGKLLRREVIAQ 540

Query: 541 VRAKM 546
           VRAKM
Sbjct: 541 VRAKM 545

BLAST of CmUC10G201130 vs. ExPASy TrEMBL
Match: A0A6J1J0P0 (4-coumarate--CoA ligase-like 7 OS=Cucurbita maxima OX=3661 GN=LOC111481607 PE=4 SV=1)

HSP 1 Score: 967.2 bits (2499), Expect = 2.8e-278
Identity = 489/545 (89.72%), Postives = 517/545 (94.86%), Query Frame = 0

Query: 1   MAITLRKSFNSQSQIYTSRRPPIHFPTDPTISIPSFLFRNSSSFPNALALADSDSGESLT 60
           MAI + KSFN QSQIYTS RPPIHFPTDPTISI SFLFRNSSSF ++LALAD+DSGESLT
Sbjct: 1   MAIAMAKSFNPQSQIYTSPRPPIHFPTDPTISILSFLFRNSSSFLHSLALADADSGESLT 60

Query: 61  FRQLQIQVSKLAHVFIQLGIRKSDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFG 120
           FRQLQIQVSKLAH FI LGIRK DVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTF 
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFA 120

Query: 121 ELSKQVANCNPKLVITVPELWDTIGKLNLPSIILGSKISSKFSRPNIWGYSDLIKKAGDV 180
           E+SKQVA+CNPKLVIT+PELWD IGKLNLPSIILGSKISSK SRPNIW YSDLIK +GDV
Sbjct: 121 EISKQVASCNPKLVITIPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV 180

Query: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQDLLGDPRNVFLCF 240
           S+LPVSEVGQ+DVAALLYSSGTTGI+KGVILTHRNFITTSLMVT DQDLLGDPRNVFLCF
Sbjct: 181 SDLPVSEVGQSDVAALLYSSGTTGINKGVILTHRNFITTSLMVTHDQDLLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSIIVYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYVVPPVIIALTKQS 300
           LPMFHVFGLS+I+YSQLQRGNTVVSMAKFELEKALGVVMKYRITHLY+VPPVIIA+ KQS
Sbjct: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300

Query: 301 VVKNYDLSSLKQILSGAAPLGKDVMEECSKFIPQARIIQGYGMTETCGVISVGNVGVEST 360
           VVK YDLSSLKQILSGAAPLGKDVMEECSK +PQ +I QGYGMTETCGVIS+ N+GVES 
Sbjct: 301 VVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESR 360

Query: 361 LSGATGSLVSGVEAQVLSTETQKRLPPGETGEICVRGPNMMKGYFNNQEATSKTIDDQGW 420
           LSG+TG LVSG+EAQ+LSTETQKRLPPGETGEICVRGPNMMKGYFNNQ+ATS+TIDDQGW
Sbjct: 361 LSGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420

Query: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPYPDDKA 480
           VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQI DAIVIPYPDDKA
Sbjct: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQIIDAIVIPYPDDKA 480

Query: 481 GEVPIAFVVRSPHSSIKEEDVKNFIAGQVAPYKRLKRVTFTSSVPKSVSGKLLRREVIAQ 540
           GEVPIAFVVRSP SSI EEDVK+FIAGQVAP+KRLK VTFTSS+PKS SGKLLRRE+IAQ
Sbjct: 481 GEVPIAFVVRSPSSSINEEDVKSFIAGQVAPFKRLKTVTFTSSIPKSASGKLLRRELIAQ 540

Query: 541 VRAKM 546
           VRAKM
Sbjct: 541 VRAKM 545

BLAST of CmUC10G201130 vs. ExPASy TrEMBL
Match: A0A6J1F2M7 (4-coumarate--CoA ligase-like 7 OS=Cucurbita moschata OX=3662 GN=LOC111441628 PE=4 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 6.9e-277
Identity = 488/545 (89.54%), Postives = 516/545 (94.68%), Query Frame = 0

Query: 1   MAITLRKSFNSQSQIYTSRRPPIHFPTDPTISIPSFLFRNSSSFPNALALADSDSGESLT 60
           MAI + KSF+ QSQIYTS RPPIHFPTDPTISI SF+FRNSSSF ++LALAD+DSGESLT
Sbjct: 1   MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFVFRNSSSFLHSLALADADSGESLT 60

Query: 61  FRQLQIQVSKLAHVFIQLGIRKSDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFG 120
           FRQLQIQVSKLAH FI LGIRK DVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTF 
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120

Query: 121 ELSKQVANCNPKLVITVPELWDTIGKLNLPSIILGSKISSKFSRPNIWGYSDLIKKAGDV 180
           ELSKQVANCNPKLVITVPELWD IGKLNLPSIILGS+ISSK SRPNIW YSDLIK +GDV
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSEISSKSSRPNIWVYSDLIKNSGDV 180

Query: 181 SNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQDLLGDPRNVFLCF 240
           S+ PVSEVGQ+DVAALLYSSGTTGISKGVILTHRNFI TSLMVT DQDLLGDPRNVFLCF
Sbjct: 181 SDFPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF 240

Query: 241 LPMFHVFGLSIIVYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYVVPPVIIALTKQS 300
           LPMFHVFGLS+I+YSQLQRGNTVVSMAKFELEKALGVVMKYRITHLY+VPPVIIA+ KQS
Sbjct: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300

Query: 301 VVKNYDLSSLKQILSGAAPLGKDVMEECSKFIPQARIIQGYGMTETCGVISVGNVGVEST 360
           VVK YDLSSLKQILSGAAPLGKDVMEECSK +PQ +I QGYGMTETCGVIS+ N+GVES 
Sbjct: 301 VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESR 360

Query: 361 LSGATGSLVSGVEAQVLSTETQKRLPPGETGEICVRGPNMMKGYFNNQEATSKTIDDQGW 420
           LSG+TG LVSG+EAQ+LSTETQKRLPPGETGEICVRGPNMMKGYFNNQ+ATS+TIDDQGW
Sbjct: 361 LSGSTGLLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420

Query: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPYPDDKA 480
           VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQ+ DAIVIPYPDDKA
Sbjct: 421 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQVIDAIVIPYPDDKA 480

Query: 481 GEVPIAFVVRSPHSSIKEEDVKNFIAGQVAPYKRLKRVTFTSSVPKSVSGKLLRREVIAQ 540
           GEVPIAFVVRSP+SSI EEDVK+FIAGQVAP+KRLK VTFTSSVPKS SGKLLRRE+IAQ
Sbjct: 481 GEVPIAFVVRSPNSSINEEDVKSFIAGQVAPFKRLKIVTFTSSVPKSASGKLLRRELIAQ 540

Query: 541 VRAKM 546
           VRAKM
Sbjct: 541 VRAKM 545

BLAST of CmUC10G201130 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 667.5 bits (1721), Expect = 8.9e-192
Identity = 337/544 (61.95%), Postives = 423/544 (77.76%), Query Frame = 0

Query: 5   LRKSFNSQSQIYTSRRPPIHFPTDPTISIPSFLFRNSSSFPNALALADSDSGESLTFRQL 64
           + KS   +  IY S RP +  P DP  S+ SFLFRNSSS+P+ LA+ADSD+G+SLTF QL
Sbjct: 1   MEKSGYGRDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQL 60

Query: 65  QIQVSKLAHVFIQLGIRKSDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFGELSK 124
           +  V++LAH F +LGIRK+DVVLIFAPNS  FP+CF A+ AIG + TT NP YT  E+SK
Sbjct: 61  KSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSK 120

Query: 125 QVANCNPKLVITVPELWDTIGKLNLPSIILGSKISSKF---SRPNIWGYSDLIKKAGDVS 184
           Q+ + NPK++I+V +L+D I   +LP ++LGSK + +    S   I  + ++++ +  VS
Sbjct: 121 QIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEPVS 180

Query: 185 NLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQDLLGDPRNVFLCFL 244
             P  E+ Q+D AALLYSSGTTG SKGV LTH NFI  SLMVT DQDL+G+   VFLCFL
Sbjct: 181 EYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLCFL 240

Query: 245 PMFHVFGLSIIVYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYVVPPVIIALTKQSV 304
           PMFHVFGL++I YSQLQRGN +VSMA+FELE  L  + K+R+THL+VVPPV +AL+KQS+
Sbjct: 241 PMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSI 300

Query: 305 VKNYDLSSLKQILSGAAPLGKDVMEECSKFIPQARIIQGYGMTETCGVISVGNVGVESTL 364
           VK +DLSSLK I SGAAPLGKD+MEEC + IP   ++QGYGMTETCG++SV +  +    
Sbjct: 301 VKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLGKRN 360

Query: 365 SGATGSLVSGVEAQVLSTETQKRLPPGETGEICVRGPNMMKGYFNNQEATSKTIDDQGWV 424
           SG+ G L  GVEAQ++S ET K  PP + GEI VRGPNMMKGY NN +AT +TID + WV
Sbjct: 361 SGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWV 420

Query: 425 HTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPYPDDKAG 484
           HTGD+GYFNE+G L+VVDRIKELIK  GFQVAPAELE LL+SHP I DA+VIP+PD++AG
Sbjct: 421 HTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAG 480

Query: 485 EVPIAFVVRSPHSSIKEEDVKNFIAGQVAPYKRLKRVTFTSSVPKSVSGKLLRREVIAQV 544
           EVPIAFVVRSP+SSI E+D++ FIA QVAPYKRL+RV+F S VPKS +GK+LRRE++ QV
Sbjct: 481 EVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQQV 540

Query: 545 RAKM 546
           R+KM
Sbjct: 541 RSKM 544

BLAST of CmUC10G201130 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 421.4 bits (1082), Expect = 1.1e-117
Identity = 224/543 (41.25%), Postives = 348/543 (64.09%), Query Frame = 0

Query: 2   AITLRKSFNSQSQIYTSRRPPIHFPTDPTISIPSFLFRNSSSFPNALALADSDSGESLTF 61
           ++  R  F + +  + S+R PI  P +P++ + +F+  +S +    +A  D+ +G++LTF
Sbjct: 3   SVNSRSGFCNSNSTFYSKRTPIPLPPNPSLDVTTFI--SSQAHRGRIAFIDASTGQNLTF 62

Query: 62  RQLQIQVSKLAHVFIQLGIRKSDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFGE 121
            +L   V  +A    ++GIRK  VVL+ +PNSI FPV   +++++GAI TT NP  T  E
Sbjct: 63  TELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNE 122

Query: 122 LSKQVANCNPKLVITVPELWDTIGKL--NLPSIILGSKISSKFSRPNIWGYSDLIKKAGD 181
           ++KQ+ + NP L  T  +L   I      LP +++  +     S  ++    +++KK   
Sbjct: 123 IAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVD--SVGDVRRLVEMMKKEPS 182

Query: 182 VSNLPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQDLLG--DPRNVF 241
             N     V Q+D A LLYSSGTTG+SKGVI +HRN I    MV    +  G  D    F
Sbjct: 183 -GNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIA---MVQTIVNRFGSDDGEQRF 242

Query: 242 LCFLPMFHVFGLSIIVYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYVVPPVIIALT 301
           +C +PMFH++GL+      L  G+T++ ++KFE+ + +  + KY+ T L +VPP+++A+ 
Sbjct: 243 ICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMV 302

Query: 302 K--QSVVKNYDLSSLKQILSGAAPLGKDVMEECSKFIPQARIIQGYGMTETCGVISVGNV 361
                +   YDLSS+  +L G APL K+V E  ++  P  +I+QGYG+TE+ G+ +  + 
Sbjct: 303 NGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDT 362

Query: 362 GVESTLSGATGSLVSGVEAQVLSTETQKRLPPGETGEICVRGPNMMKGYFNNQEATSKTI 421
             ES   G  G L + +E +++   T + L P +TGE+ ++GP++MKGYF+N+EATS T+
Sbjct: 363 VEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTL 422

Query: 422 DDQGWVHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPY 481
           D +GW+ TGD+ Y +E+G +FVVDR+KELIK  G+QVAPAELEALLL+HP+ITDA VIP+
Sbjct: 423 DSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPF 482

Query: 482 PDDKAGEVPIAFVVRSPHSSIKEEDVKNFIAGQVAPYKRLKRVTFTSSVPKSVSGKLLRR 539
           PD + G+ P+A+VVR   SS+ E+ +  F+A QVAPYKR+++V F SS+PK+ SGK+LR+
Sbjct: 483 PDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRK 537

BLAST of CmUC10G201130 vs. TAIR 10
Match: AT4G19010.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 408.3 bits (1048), Expect = 9.7e-114
Identity = 226/537 (42.09%), Postives = 342/537 (63.69%), Query Frame = 0

Query: 9   FNSQSQIYTSRRPPIHFPTDPTISIPSFLFRNSSSFPNALALADSDSGESLTFRQLQIQV 68
           F+S++ IYTS+ P +H P DP +   S LF  S       AL DS +G S++  +LQI V
Sbjct: 25  FSSKTGIYTSKFPSLHLPVDPNLDAVSALF--SHKHHGDTALIDSLTGFSISHTELQIMV 84

Query: 69  -SKLAHVFIQLGIRKSDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFGELSKQVA 128
            S  A ++  LG+R+ DVV +  PNS++FP+ F +++++GAI TT NP+ + GE+ KQV+
Sbjct: 85  QSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPSSSLGEIKKQVS 144

Query: 129 NCNPKLVITVPELWDTIGKLNLPSIILGSKI---SSKFSRPNIWGYSDLIKKAGDVSNLP 188
            C+  L  T  E  + +  L +  I +       S +   P    YS + +  G V   P
Sbjct: 145 ECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIRIENPKF--YSIMKESFGFV---P 204

Query: 189 VSEVGQNDVAALLYSSGTTGISKGVILTHRNFITT-SLMVTQDQDLLGDP--RNVFLCFL 248
              + Q+DVAA++YSSGTTG SKGV+LTHRN I +  L V  +      P   NV+L  L
Sbjct: 205 KPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSSNVYLAAL 264

Query: 249 PMFHVFGLSIIVYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYVVPPVIIALTKQSV 308
           P+ H++GLS+ V   L  G+T+V M +F+    + V+ +++ITH  VVPP+++ALTK++ 
Sbjct: 265 PLCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPMLMALTKKAK 324

Query: 309 -VKNYDLSSLKQILSGAAPLGKDVMEECSKFIPQARIIQGYGMTETCGVISVGNVGVEST 368
            V      SLKQ+ SGAAPL +  +E+  + +P   +IQGYGMTE+  V + G    + +
Sbjct: 325 GVCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGTRGFNSEKLS 384

Query: 369 LSGATGSLVSGVEAQVLSTETQKRLPPGETGEICVRGPNMMKGYFNNQEATSKTIDDQGW 428
              + G L   ++A+V+   +   LPPG  GE+ ++GP +MKGY NN +AT  +I +  W
Sbjct: 385 RYSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKATQMSIVEDSW 444

Query: 429 VHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPYPDDKA 488
           + TGDI YF+E+G LF+VDRIKE+IK  GFQ+APA+LEA+L+SHP I DA V   P+++ 
Sbjct: 445 LRTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAVTAAPNEEC 504

Query: 489 GEVPIAFVVRSPHSSIKEEDVKNFIAGQVAPYKRLKRVTFTSSVPKSVSGKLLRREV 538
           GE+P+AFVVR   +++ EEDV +++A QVAPY+++++V   +S+PKS +GK+LR+E+
Sbjct: 505 GEIPVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTGKILRKEL 554

BLAST of CmUC10G201130 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 396.0 bits (1016), Expect = 5.0e-110
Identity = 227/539 (42.12%), Postives = 336/539 (62.34%), Query Frame = 0

Query: 14  QIYTSRRPPIHFPTDPTISIPSFLFRNSSSFPNALALADSDSGESLTFRQLQIQVSKLAH 73
           +I+ S+ P I  P    + + ++ F   SS  +   L    +G+S T+ +  +   ++A 
Sbjct: 33  RIFRSKLPDIDIPNH--LPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVAS 92

Query: 74  VFIQLGIRKSDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFGELSKQVANCNPKL 133
              +LGIRK DV++I   NS  F   F     IGA++TT NP YT  EL KQ+ +   KL
Sbjct: 93  GLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKL 152

Query: 134 VITVPELWDTIGKLNLPSIILGSKISSKFSRPNIWGYSDLIKKAGDVSNLPVSEVGQNDV 193
           +IT  +  D +  L     ++    + + +  N   +S LI            ++G +D 
Sbjct: 153 IITHSQYVDKLKNLGENLTLI---TTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDA 212

Query: 194 AALLYSSGTTGISKGVILTHRNFITTSLMVTQDQDLLGDPRN-------VFLCFLPMFHV 253
           AAL +SSGTTG+ KGV+LTH++ IT+   V Q  D  GD  N       V LC LP+FH+
Sbjct: 213 AALPFSSGTTGLPKGVVLTHKSLITS---VAQQVD--GDNPNLYLKSNDVILCVLPLFHI 272

Query: 254 FGLSIIVYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYVVPPVIIALTKQSVVKNYD 313
           + L+ ++ + L+ G TV+ M KFE+   L ++ ++R+T   +VPP++IAL K   V +YD
Sbjct: 273 YSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYD 332

Query: 314 LSSLKQILSGAAPLGKDVMEECSKFIPQARIIQGYGMTETCGVISVGNVGVES----TLS 373
           LSS++ +LSGAAPLGK++ +   + +PQA + QGYGMTE   V+S+ ++G       T S
Sbjct: 333 LSSVRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSM-SLGFAKEPIPTKS 392

Query: 374 GATGSLVSGVEAQVLSTETQKRLPPGETGEICVRGPNMMKGYFNNQEATSKTIDDQGWVH 433
           G+ G++V   E +V+  ET+  L   + GEIC+RG  +MK Y N+ EATS TID++GW+H
Sbjct: 393 GSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLH 452

Query: 434 TGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQITDAIVIPYPDDKAGE 493
           TGDIGY +E+ E+F+VDR+KE+IK  GFQV PAELE+LL++H  I DA V+P  D+ AGE
Sbjct: 453 TGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGE 512

Query: 494 VPIAFVVRSPHSSIKEEDVKNFIAGQVAPYKRLKRVTFTSSVPKSVSGKLLRREVIAQV 542
           VP+AFVVRS  + I EEDVK ++A QV  YKRL +V F +S+PKS SGK+LR+++ A++
Sbjct: 513 VPVAFVVRSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of CmUC10G201130 vs. TAIR 10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 394.8 bits (1013), Expect = 1.1e-109
Identity = 217/559 (38.82%), Postives = 340/559 (60.82%), Query Frame = 0

Query: 1   MAITLRKSFNSQSQIYTSRRPPIHFPTDPTISIPSFLFRNSSSFPNALALADSDSGESLT 60
           +A+  +  F   + I+ S+R P+  P +  + + SF+   S          D+ +G  L+
Sbjct: 19  LAVDRKSGFCESTSIFYSKREPMALPPNQFLDVTSFI--ASQPHRGKTVFVDAVTGRRLS 78

Query: 61  FRQLQIQVSKLAHVFIQLGIRKSDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFG 120
           F +L + V ++A     LG+RK +VV+I +PNSI FP+   +++++GAI TT NP  T  
Sbjct: 79  FPELWLGVERVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSD 138

Query: 121 ELSKQVANCNPKLVITVPELWDTI---GKLNLPSIILGSKISSKFSRPNIWGYSDLIKKA 180
           E+SKQ+ +  P L  T  +L   +      NLP +++       +  P+   Y D +K  
Sbjct: 139 EISKQIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLM-----DDYHVPS-QSYGDRVKLV 198

Query: 181 GDVSNLPVSE---------VGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQD 240
           G +  +  +E         V Q+D AALLYSSGTTG SKGV+L+HRN I    +V   + 
Sbjct: 199 GRLETMIETEPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIA---LVQAYRA 258

Query: 241 LLGDPRNVFLCFLPMFHVFGLSIIVYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYV 300
             G  +   +C +PM H+FG        +  G T+V + KF++ K L  V  +R ++L +
Sbjct: 259 RFGLEQRT-ICTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSL 318

Query: 301 VPPVIIALTK--QSVVKNYDLSSLKQILSGAAPLGKDVMEECSKFIPQARIIQGYGMTET 360
           VPP+++A+      +   YDLSSL  +++G APL ++V E+  +  P+ +I+QGYG+TE+
Sbjct: 319 VPPIVVAMVNGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTES 378

Query: 361 CGVISVGNVGVESTLSGATGSLVSGVEAQVLSTETQKRLPPGETGEICVRGPNMMKGYFN 420
             + +      E+   GA+G L   VE +++  +T + L   +TGE+ +R P +MKGYF 
Sbjct: 379 TAIAASMFNKEETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFK 438

Query: 421 NQEATSKTIDDQGWVHTGDIGYFNEEGELFVVDRIKELIKCYGFQVAPAELEALLLSHPQ 480
           N+EAT+ TID +GW+ TGD+ Y + +G +FVVDR+KELIKC G+QVAPAELEALLL+HP+
Sbjct: 439 NKEATASTIDSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPE 498

Query: 481 ITDAIVIPYPDDKAGEVPIAFVVRSPHSSIKEEDVKNFIAGQVAPYKRLKRVTFTSSVPK 540
           I DA VIP PD KAG+ P+A++VR   S++ E ++  F+A QV+PYK++++VTF +S+PK
Sbjct: 499 IADAAVIPIPDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIPK 558

Query: 541 SVSGKLLRREVIAQVRAKM 546
           + SGK+LRRE+     +K+
Sbjct: 559 NPSGKILRRELTKLTTSKL 565

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038905825.12.1e-28892.664-coumarate--CoA ligase-like 7 isoform X1 [Benincasa hispida][more]
XP_011651966.21.6e-28392.114-coumarate--CoA ligase-like 7 [Cucumis sativus] >KAE8651043.1 hypothetical prot... [more]
XP_008442710.13.0e-28291.74PREDICTED: 4-coumarate--CoA ligase-like 7 [Cucumis melo] >KAA0043992.1 4-coumara... [more]
XP_022982901.15.8e-27889.724-coumarate--CoA ligase-like 7 [Cucurbita maxima][more]
KAG6580619.19.9e-27890.094-coumarate--CoA ligase-like 7, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
M4IQS11.3e-21167.22Probable CoA ligase CCL10 OS=Humulus lupulus OX=3486 GN=CCL10 PE=2 SV=1[more]
M4IRL63.0e-19262.13Probable CoA ligase CCL7 OS=Humulus lupulus OX=3486 GN=CCL7 PE=2 SV=1[more]
Q9M0X91.3e-19061.954-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=... [more]
Q0DV324.1e-15755.394-coumarate--CoA ligase-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL1... [more]
M4IQR72.5e-12243.30Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LDK03.4e-28492.11Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G740270 PE=4 SV=1[more]
A0A5A7TKZ41.4e-28291.744-coumarate--CoA ligase-like 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3B5U81.4e-28291.744-coumarate--CoA ligase-like 7 OS=Cucumis melo OX=3656 GN=LOC103486502 PE=4 SV=1[more]
A0A6J1J0P02.8e-27889.724-coumarate--CoA ligase-like 7 OS=Cucurbita maxima OX=3661 GN=LOC111481607 PE=4 ... [more]
A0A6J1F2M76.9e-27789.544-coumarate--CoA ligase-like 7 OS=Cucurbita moschata OX=3662 GN=LOC111441628 PE=... [more]
Match NameE-valueIdentityDescription
AT4G05160.18.9e-19261.95AMP-dependent synthetase and ligase family protein [more]
AT1G20510.11.1e-11741.25OPC-8:0 CoA ligase1 [more]
AT4G19010.19.7e-11442.09AMP-dependent synthetase and ligase family protein [more]
AT1G65060.15.0e-11042.124-coumarate:CoA ligase 3 [more]
AT1G20480.11.1e-10938.82AMP-dependent synthetase and ligase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 13..442
e-value: 1.0E-129
score: 435.0
NoneNo IPR availableGENE3D3.30.300.30coord: 443..544
e-value: 1.1E-28
score: 101.9
NoneNo IPR availablePANTHERPTHR24096LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 8..544
NoneNo IPR availablePANTHERPTHR24096:SF3734-COUMARATE:COA LIGASE-LIKE PROTEINcoord: 8..544
NoneNo IPR availableCDDcd059044CLcoord: 25..536
e-value: 0.0
score: 722.87
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 17..543
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 39..447
e-value: 3.8E-105
score: 351.9
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 456..531
e-value: 2.1E-14
score: 54.2
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 196..207

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmUC10G201130.1CmUC10G201130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005829 cytosol
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0016405 CoA-ligase activity