CmUC10G186550 (gene) Watermelon (USVL531) v1

BLAST of CmUC10G186550 vs. NCBI nr
Match: KAG7015576.1 (Poly(A)-specific ribonuclease PARN [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1215.3 bits (3143), Expect = 0.0e+00
Identity = 627/753 (83.27%), Postives = 666/753 (88.45%), Query Frame = 0

Query: 1   MDPSRSLSALPRLLCFIRTAAYSSAAVTAPTIASNSSFPLKNVTKLNFEAALSELRTHVR 60
           M+ SR L A  RLLCFIRTA YSSAAVTAP  ASNS FPLKNVTK NFEA LSELR+HVR
Sbjct: 1   MNLSRPLWAPTRLLCFIRTATYSSAAVTAPATASNSCFPLKNVTKSNFEAVLSELRSHVR 60

Query: 61  AADFVAVDLEMTGITSAPWRESLEFDRFDIRYLKVKDSAEKFAVVQFGVCPFRWDSAKNS 120
           AADFVA+DLEMTGITSAPWRES EFDRFD+RYLKVKDSAEKFAVVQFGVCPFRWDSAKNS
Sbjct: 61  AADFVAIDLEMTGITSAPWRESFEFDRFDVRYLKVKDSAEKFAVVQFGVCPFRWDSAKNS 120

Query: 121 FVAHPHNFFVFPRHEIQVDGPSSEFLCQTTSMEFLAKYQFDFNACIREGISYLSRAHEDA 180
           FVAHPHNFFVFPRHEIQVDGPSSEFLCQTTS+EFLAKYQFDFNACI EGISYLSRAHEDA
Sbjct: 121 FVAHPHNFFVFPRHEIQVDGPSSEFLCQTTSIEFLAKYQFDFNACIHEGISYLSRAHEDA 180

Query: 181 ARKRLNFAYGDEMSDASSNV--IRDKKRIGIADVLFKERMKNRLHEWRDRLLSERNMEFQ 240
           ARKRLNFA+GDE SDASSNV  IRD+KRIGIADVLFKERMKNRLHEWRDRLLSERNME Q
Sbjct: 181 ARKRLNFAHGDETSDASSNVTNIRDQKRIGIADVLFKERMKNRLHEWRDRLLSERNMECQ 240

Query: 241 GQEGSNDYQRQFQTIFFKMRPALTLSDFTSHQIRLIRLKLSVSDIKYFSNSTFCYLFIKS 300
           GQEGSN+YQRQF+TIFFKMRPALTLSDFTSHQ +LI+L                      
Sbjct: 241 GQEGSNNYQRQFETIFFKMRPALTLSDFTSHQTKLIQL---------------------- 300

Query: 301 YPCLAACEHMQVIKKHFEDLCFVVVNDGDDNLQQLVVYVDSRDDKELLMQEVNSDQKEAA 360
                      VIKKHFEDLCFVVV+DGD NLQQLVVYVDSRDDKELLMQEVNSD++EAA
Sbjct: 301 -----------VIKKHFEDLCFVVVDDGDANLQQLVVYVDSRDDKELLMQEVNSDRQEAA 360

Query: 361 EMKIQSAIGFRHVIDLLASEKKLIIGHNCFLADTSVFGPDIAHIHTKFIGSLPSTAEEFV 420
           EMKIQSAIGFRHVIDLL+SEKKLIIGHNCFL        DIAHIH KFIGSLPSTAEEFV
Sbjct: 361 EMKIQSAIGFRHVIDLLSSEKKLIIGHNCFL--------DIAHIHKKFIGSLPSTAEEFV 420

Query: 421 SSIGKHFPYIMDTKILLNGNSILQRRMKKSSTSLSSAFGLLCPHIAFSSQSNPAFKSGVR 480
           SSIGKHFP+IMDTKILLNGN +LQ+RMKKSSTSLSSAF LLCPHIAFSSQSNPAF SGV+
Sbjct: 421 SSIGKHFPFIMDTKILLNGNGVLQQRMKKSSTSLSSAFALLCPHIAFSSQSNPAFSSGVQ 480

Query: 481 VEVQVDDLRSSNWNSGDKHEAGYDAFMTGCVFAQACNLIGIDFALRSPSENLVDNENLQK 540
           VEVQVDDL SSNWNSGDKHEAGYDAFMTGCVFAQACNL+GIDF L  PSENL DNE LQK
Sbjct: 481 VEVQVDDLSSSNWNSGDKHEAGYDAFMTGCVFAQACNLMGIDFVL--PSENLADNEKLQK 540

Query: 541 HVNHLYLSWASGDIIDLSTGNRMTESLRSNRSKKHQAKISFENIVLIWGFPTHLNAREIK 600
           H+NHLYLSWASGDIIDLSTGNRMTE LRSNRSKKHQAKISFENIVLIWGFP +LNAR+IK
Sbjct: 541 HINHLYLSWASGDIIDLSTGNRMTEPLRSNRSKKHQAKISFENIVLIWGFPANLNARKIK 600

Query: 601 ECISKVFGLTNVVSVYHLDESAVFVQFAKPEMVSEFLDLKENLERHDDPISVLHPLTKLF 660
           ECISKVFGL N+VSVYH+DESAVFVQFAKPEMVSEFLDLK+NLERHDDPISVLHPLT+LF
Sbjct: 601 ECISKVFGLANIVSVYHIDESAVFVQFAKPEMVSEFLDLKKNLERHDDPISVLHPLTELF 660

Query: 661 EARTTHAAGYEAYKKICSSPISKLLFADQAEAVGINQKTALIKSEAVKSQMHEA------ 720
            A++THAAGYEAYK+ICSSP+ K+LFADQAEAVGINQKT  IK E+VKSQMHEA      
Sbjct: 661 GAKSTHAAGYEAYKEICSSPMFKVLFADQAEAVGINQKTTSIKPESVKSQMHEAANSSLT 710

Query: 721 VEMLNETTEIQGMEMGSNDLSSTNYHSLRSSSM 746
            +MLNETTE+QGME+G+NDL +TNYH L+SSSM
Sbjct: 721 ADMLNETTEVQGMEVGANDLRNTNYHILQSSSM 710

BLAST of CmUC10G186550 vs. NCBI nr
Match: XP_022932379.1 (poly(A)-specific ribonuclease PARN isoform X1 [Cucurbita moschata])

HSP 1 Score: 1213.0 bits (3137), Expect = 0.0e+00
Identity = 628/753 (83.40%), Postives = 664/753 (88.18%), Query Frame = 0

Query: 1   MDPSRSLSALPRLLCFIRTAAYSSAAVTAPTIASNSSFPLKNVTKLNFEAALSELRTHVR 60
           M+ SR L A  RLLCFIRTA YSSAAVT P  ASNSSFPLKNVTK NFEA LSELR+HVR
Sbjct: 1   MNLSRPLWAPTRLLCFIRTATYSSAAVTTPATASNSSFPLKNVTKSNFEAVLSELRSHVR 60

Query: 61  AADFVAVDLEMTGITSAPWRESLEFDRFDIRYLKVKDSAEKFAVVQFGVCPFRWDSAKNS 120
           AADFVA+DLEMTGITSAPWRES EFDRFD+RYLKVKDSAEKFAVVQFGVCPFRWDSAKNS
Sbjct: 61  AADFVAIDLEMTGITSAPWRESFEFDRFDVRYLKVKDSAEKFAVVQFGVCPFRWDSAKNS 120

Query: 121 FVAHPHNFFVFPRHEIQVDGPSSEFLCQTTSMEFLAKYQFDFNACIREGISYLSRAHEDA 180
           FVAHPHNFFVFPRHEI VDGPSSEFLCQTTS+EFLAKYQFDFNACI EGISYLSRAHEDA
Sbjct: 121 FVAHPHNFFVFPRHEILVDGPSSEFLCQTTSIEFLAKYQFDFNACIHEGISYLSRAHEDA 180

Query: 181 ARKRLNFAYGDEMSDASSNV--IRDKKRIGIADVLFKERMKNRLHEWRDRLLSERNMEFQ 240
           ARKRLNFA+GDE SDASSNV  IRD+KRIGIADVLFKERMKNRLHEWRDRLLSERNME Q
Sbjct: 181 ARKRLNFAHGDETSDASSNVTNIRDQKRIGIADVLFKERMKNRLHEWRDRLLSERNMECQ 240

Query: 241 GQEGSNDYQRQFQTIFFKMRPALTLSDFTSHQIRLIRLKLSVSDIKYFSNSTFCYLFIKS 300
           GQEGSNDYQRQF+TIFFKMRPALTLSDFTSHQ +LI+L                      
Sbjct: 241 GQEGSNDYQRQFETIFFKMRPALTLSDFTSHQTKLIQL---------------------- 300

Query: 301 YPCLAACEHMQVIKKHFEDLCFVVVNDGDDNLQQLVVYVDSRDDKELLMQEVNSDQKEAA 360
                      VIKKHFEDLCFVVV+DGD NLQQLVVYVDSRDDKELLMQEVNSD++EAA
Sbjct: 301 -----------VIKKHFEDLCFVVVDDGDANLQQLVVYVDSRDDKELLMQEVNSDRQEAA 360

Query: 361 EMKIQSAIGFRHVIDLLASEKKLIIGHNCFLADTSVFGPDIAHIHTKFIGSLPSTAEEFV 420
           EMKIQSAIGFRHVIDLL+SEKKLIIGHNCFL        DIAHIH KFIGSLPSTAEEFV
Sbjct: 361 EMKIQSAIGFRHVIDLLSSEKKLIIGHNCFL--------DIAHIHKKFIGSLPSTAEEFV 420

Query: 421 SSIGKHFPYIMDTKILLNGNSILQRRMKKSSTSLSSAFGLLCPHIAFSSQSNPAFKSGVR 480
           SSIGKHFP+IMDTKILLNGN ILQ+RMKKSSTSLSSAF LLCPHIAFSSQSNPAF SGV+
Sbjct: 421 SSIGKHFPFIMDTKILLNGNGILQQRMKKSSTSLSSAFALLCPHIAFSSQSNPAFSSGVQ 480

Query: 481 VEVQVDDLRSSNWNSGDKHEAGYDAFMTGCVFAQACNLIGIDFALRSPSENLVDNENLQK 540
           VEVQVDDL SSNWNSGDKHEAGYDAFMTGCVFAQACNL+GIDF L  PSENL DNE LQK
Sbjct: 481 VEVQVDDLSSSNWNSGDKHEAGYDAFMTGCVFAQACNLMGIDFVL--PSENLADNEKLQK 540

Query: 541 HVNHLYLSWASGDIIDLSTGNRMTESLRSNRSKKHQAKISFENIVLIWGFPTHLNAREIK 600
           H+NHLYLSWASGDIIDLSTGNRMTE LRSNRSKKHQAKISFENIVLIWGFP +LNAR+IK
Sbjct: 541 HINHLYLSWASGDIIDLSTGNRMTEPLRSNRSKKHQAKISFENIVLIWGFPANLNARKIK 600

Query: 601 ECISKVFGLTNVVSVYHLDESAVFVQFAKPEMVSEFLDLKENLERHDDPISVLHPLTKLF 660
           ECISKVFGL N+VSVYH+DESAVFVQFAKPEMVSEFLDLK+NLERHDDPISVLHPLT+LF
Sbjct: 601 ECISKVFGLANIVSVYHIDESAVFVQFAKPEMVSEFLDLKKNLERHDDPISVLHPLTELF 660

Query: 661 EARTTHAAGYEAYKKICSSPISKLLFADQAEAVGINQKTALIKSEAVKSQMHEA------ 720
            A+ T+AAGYEAYK+ICSSP+ K+LFADQAEAVGINQKT  IK E+VKSQMHEA      
Sbjct: 661 GAKGTNAAGYEAYKEICSSPMFKVLFADQAEAVGINQKTTSIKPESVKSQMHEAANNSLT 710

Query: 721 VEMLNETTEIQGMEMGSNDLSSTNYHSLRSSSM 746
            +MLNETTE+QGME+G+NDL +TNYH LRSSSM
Sbjct: 721 ADMLNETTEVQGMEVGANDLRNTNYHILRSSSM 710

BLAST of CmUC10G186550 vs. NCBI nr
Match: XP_023553318.1 (poly(A)-specific ribonuclease PARN isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1213.0 bits (3137), Expect = 0.0e+00
Identity = 627/753 (83.27%), Postives = 663/753 (88.05%), Query Frame = 0

Query: 1   MDPSRSLSALPRLLCFIRTAAYSSAAVTAPTIASNSSFPLKNVTKLNFEAALSELRTHVR 60
           M+ SR L A  RLLCF RTA YSSAAV++P  ASNSSFPLKNVTK NFEA LSELR+HVR
Sbjct: 1   MNLSRPLWAPTRLLCFTRTATYSSAAVSSPATASNSSFPLKNVTKSNFEAVLSELRSHVR 60

Query: 61  AADFVAVDLEMTGITSAPWRESLEFDRFDIRYLKVKDSAEKFAVVQFGVCPFRWDSAKNS 120
           AADFVA+DLEMTGITSAPWRES EFDRFD+RYLKVKDSAEKFAVVQFGVCPFRWDSAKNS
Sbjct: 61  AADFVAIDLEMTGITSAPWRESFEFDRFDVRYLKVKDSAEKFAVVQFGVCPFRWDSAKNS 120

Query: 121 FVAHPHNFFVFPRHEIQVDGPSSEFLCQTTSMEFLAKYQFDFNACIREGISYLSRAHEDA 180
           FVAHPHNFFVFPRHEIQVDGPSSEFLCQTTS+EFLAKYQFDFNACI EGISYLSRAHEDA
Sbjct: 121 FVAHPHNFFVFPRHEIQVDGPSSEFLCQTTSIEFLAKYQFDFNACIHEGISYLSRAHEDA 180

Query: 181 ARKRLNFAYGDEMSDASSNV--IRDKKRIGIADVLFKERMKNRLHEWRDRLLSERNMEFQ 240
           ARKRLNFA+GDE  DASSNV  IRD KRIGIADVLFKERMKNRLHEWRDRLLSERNME Q
Sbjct: 181 ARKRLNFAHGDETLDASSNVTNIRDPKRIGIADVLFKERMKNRLHEWRDRLLSERNMECQ 240

Query: 241 GQEGSNDYQRQFQTIFFKMRPALTLSDFTSHQIRLIRLKLSVSDIKYFSNSTFCYLFIKS 300
           GQEGSNDYQRQF+TIFFKMRPALTLSDFTSHQ +LI+L                      
Sbjct: 241 GQEGSNDYQRQFETIFFKMRPALTLSDFTSHQTKLIQL---------------------- 300

Query: 301 YPCLAACEHMQVIKKHFEDLCFVVVNDGDDNLQQLVVYVDSRDDKELLMQEVNSDQKEAA 360
                      VIKKHFEDLCFVVV+DGD NLQQLVVYVDSRDDKELLMQEVNSDQ+EAA
Sbjct: 301 -----------VIKKHFEDLCFVVVDDGDANLQQLVVYVDSRDDKELLMQEVNSDQREAA 360

Query: 361 EMKIQSAIGFRHVIDLLASEKKLIIGHNCFLADTSVFGPDIAHIHTKFIGSLPSTAEEFV 420
           EMKIQSAIGFRHVIDLL+SEKKLIIGHNCFL        DIAHIH KFIGSLPSTAEEFV
Sbjct: 361 EMKIQSAIGFRHVIDLLSSEKKLIIGHNCFL--------DIAHIHKKFIGSLPSTAEEFV 420

Query: 421 SSIGKHFPYIMDTKILLNGNSILQRRMKKSSTSLSSAFGLLCPHIAFSSQSNPAFKSGVR 480
           SSIGKHFP+IMDTKILLNGN ILQ+RMKKSSTSLSSAF LLCPHIAFSSQSNPAF SGV+
Sbjct: 421 SSIGKHFPFIMDTKILLNGNGILQQRMKKSSTSLSSAFALLCPHIAFSSQSNPAFSSGVQ 480

Query: 481 VEVQVDDLRSSNWNSGDKHEAGYDAFMTGCVFAQACNLIGIDFALRSPSENLVDNENLQK 540
           VEVQVDDL SSNWNSGDKHEAGYDAFMTGCVFAQACNL+GIDF L  PSENL DNE LQK
Sbjct: 481 VEVQVDDLSSSNWNSGDKHEAGYDAFMTGCVFAQACNLMGIDFVL--PSENLADNEKLQK 540

Query: 541 HVNHLYLSWASGDIIDLSTGNRMTESLRSNRSKKHQAKISFENIVLIWGFPTHLNAREIK 600
           H+NHLYLSWASGDIIDLSTGNR TE LRSNRSKKHQAKISFENIVLIWGFP +LNAR+IK
Sbjct: 541 HINHLYLSWASGDIIDLSTGNRTTEPLRSNRSKKHQAKISFENIVLIWGFPANLNARKIK 600

Query: 601 ECISKVFGLTNVVSVYHLDESAVFVQFAKPEMVSEFLDLKENLERHDDPISVLHPLTKLF 660
           ECISKVFGL N+VSVYH+DESAVFVQFAKPEMVSEFLDLK+NLERHDDPISVLHPLT+LF
Sbjct: 601 ECISKVFGLANIVSVYHIDESAVFVQFAKPEMVSEFLDLKKNLERHDDPISVLHPLTELF 660

Query: 661 EARTTHAAGYEAYKKICSSPISKLLFADQAEAVGINQKTALIKSEAVKSQMHEA------ 720
            A++THAAGYEAYK+ICSSP+ K+LFADQAEAVGINQKT  IK E+VKSQMHEA      
Sbjct: 661 GAKSTHAAGYEAYKEICSSPMFKVLFADQAEAVGINQKTTSIKPESVKSQMHEAANNNLT 710

Query: 721 VEMLNETTEIQGMEMGSNDLSSTNYHSLRSSSM 746
            +MLNETTE+QGME+G+NDL +TNYH LRSSSM
Sbjct: 721 ADMLNETTEVQGMEVGANDLRNTNYHILRSSSM 710

BLAST of CmUC10G186550 vs. NCBI nr
Match: KAG6577517.1 (Poly(A)-specific ribonuclease PARN, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1209.1 bits (3127), Expect = 0.0e+00
Identity = 624/753 (82.87%), Postives = 664/753 (88.18%), Query Frame = 0

Query: 1   MDPSRSLSALPRLLCFIRTAAYSSAAVTAPTIASNSSFPLKNVTKLNFEAALSELRTHVR 60
           M+ SR L A  RLLCFIRTA YSSAAVTAP  ASNS FPLKNVTK NFEA LSELR+HVR
Sbjct: 1   MNLSRPLWAPTRLLCFIRTATYSSAAVTAPATASNSCFPLKNVTKSNFEAVLSELRSHVR 60

Query: 61  AADFVAVDLEMTGITSAPWRESLEFDRFDIRYLKVKDSAEKFAVVQFGVCPFRWDSAKNS 120
           AADFVA+DLEMTGITSAPWRES EFDRFD+RYLKVKDSAEKFAVVQFGVCPFRWDSAKNS
Sbjct: 61  AADFVAIDLEMTGITSAPWRESFEFDRFDVRYLKVKDSAEKFAVVQFGVCPFRWDSAKNS 120

Query: 121 FVAHPHNFFVFPRHEIQVDGPSSEFLCQTTSMEFLAKYQFDFNACIREGISYLSRAHEDA 180
           FVAHPHNFFVFPRHEIQVDGPSSEFLCQTTS+EFLAKYQFDFN CI EGISYLSRAHEDA
Sbjct: 121 FVAHPHNFFVFPRHEIQVDGPSSEFLCQTTSIEFLAKYQFDFNFCIHEGISYLSRAHEDA 180

Query: 181 ARKRLNFAYGDEMSDASSNV--IRDKKRIGIADVLFKERMKNRLHEWRDRLLSERNMEFQ 240
           ARKRLNFA+GDE SDASSNV  IRD+KRIGIADVLFKERMKNRLHEWRDRLLSERNME Q
Sbjct: 181 ARKRLNFAHGDETSDASSNVTNIRDQKRIGIADVLFKERMKNRLHEWRDRLLSERNMECQ 240

Query: 241 GQEGSNDYQRQFQTIFFKMRPALTLSDFTSHQIRLIRLKLSVSDIKYFSNSTFCYLFIKS 300
           GQEGSN+YQRQF+TIFFKMRPALTLSDFTSHQ +LI+L                      
Sbjct: 241 GQEGSNNYQRQFETIFFKMRPALTLSDFTSHQTKLIQL---------------------- 300

Query: 301 YPCLAACEHMQVIKKHFEDLCFVVVNDGDDNLQQLVVYVDSRDDKELLMQEVNSDQKEAA 360
                      VIKKHFEDLCF+VV+DGD NLQQLVVYVDSRDDKELLMQEVNSD++EAA
Sbjct: 301 -----------VIKKHFEDLCFIVVDDGDANLQQLVVYVDSRDDKELLMQEVNSDRQEAA 360

Query: 361 EMKIQSAIGFRHVIDLLASEKKLIIGHNCFLADTSVFGPDIAHIHTKFIGSLPSTAEEFV 420
           EMKIQSAIGFRHVIDLL+SEKKLIIGHNCFL        DIAHIH KFIGSLPSTAEEFV
Sbjct: 361 EMKIQSAIGFRHVIDLLSSEKKLIIGHNCFL--------DIAHIHKKFIGSLPSTAEEFV 420

Query: 421 SSIGKHFPYIMDTKILLNGNSILQRRMKKSSTSLSSAFGLLCPHIAFSSQSNPAFKSGVR 480
           SSIGKHFP+IMDTKILLNGN +LQ+RMKKSSTSLSSAF LLCPHIAFSSQSNPAF SGV+
Sbjct: 421 SSIGKHFPFIMDTKILLNGNGVLQQRMKKSSTSLSSAFALLCPHIAFSSQSNPAFSSGVQ 480

Query: 481 VEVQVDDLRSSNWNSGDKHEAGYDAFMTGCVFAQACNLIGIDFALRSPSENLVDNENLQK 540
           VEVQVDDL SSNWNSGDKHEAGYDAFMTGCVFAQACNL+GIDF L  PSENL DNE LQK
Sbjct: 481 VEVQVDDLSSSNWNSGDKHEAGYDAFMTGCVFAQACNLMGIDFVL--PSENLADNEKLQK 540

Query: 541 HVNHLYLSWASGDIIDLSTGNRMTESLRSNRSKKHQAKISFENIVLIWGFPTHLNAREIK 600
           H+NHLYLSWASGDIIDLSTGNRMTE LRSNRSKKHQAKISFENIVLIWGFP +LNAR+IK
Sbjct: 541 HINHLYLSWASGDIIDLSTGNRMTEPLRSNRSKKHQAKISFENIVLIWGFPANLNARKIK 600

Query: 601 ECISKVFGLTNVVSVYHLDESAVFVQFAKPEMVSEFLDLKENLERHDDPISVLHPLTKLF 660
           ECISKVFGL N+VSVYH+DESAVFVQFAKPEMVSEFLDLK+ LERHDDPISVLHPLT+LF
Sbjct: 601 ECISKVFGLANIVSVYHIDESAVFVQFAKPEMVSEFLDLKKILERHDDPISVLHPLTELF 660

Query: 661 EARTTHAAGYEAYKKICSSPISKLLFADQAEAVGINQKTALIKSEAVKSQMHEA------ 720
            A++THAAGYEAYK+ICSSP+ K+LFADQAEAVGINQKT  IK E+VKSQMHEA      
Sbjct: 661 GAKSTHAAGYEAYKEICSSPMFKVLFADQAEAVGINQKTTSIKPESVKSQMHEAANSSLT 710

Query: 721 VEMLNETTEIQGMEMGSNDLSSTNYHSLRSSSM 746
            +MLNETTE+QGME+G+NDL +TNYH L+SSSM
Sbjct: 721 ADMLNETTEVQGMEVGANDLRNTNYHILQSSSM 710

BLAST of CmUC10G186550 vs. NCBI nr
Match: XP_023007721.1 (poly(A)-specific ribonuclease PARN isoform X1 [Cucurbita maxima])

HSP 1 Score: 1197.6 bits (3097), Expect = 0.0e+00
Identity = 620/753 (82.34%), Postives = 659/753 (87.52%), Query Frame = 0

Query: 1   MDPSRSLSALPRLLCFIRTAAYSSAAVTAPTIASNSSFPLKNVTKLNFEAALSELRTHVR 60
           M+ SR L A  RLLCFIRTA YSSAAVTAP  ASNSSFPLKNVTK NFEA LSELR+HVR
Sbjct: 1   MNLSRPLWAPTRLLCFIRTATYSSAAVTAPATASNSSFPLKNVTKSNFEAVLSELRSHVR 60

Query: 61  AADFVAVDLEMTGITSAPWRESLEFDRFDIRYLKVKDSAEKFAVVQFGVCPFRWDSAKNS 120
           AADFVA+DLEMTGITSAPWRES EFDRFD+RYLKVKDSAEKFAVVQFGVCPFRWDSAKNS
Sbjct: 61  AADFVAIDLEMTGITSAPWRESFEFDRFDVRYLKVKDSAEKFAVVQFGVCPFRWDSAKNS 120

Query: 121 FVAHPHNFFVFPRHEIQVDGPSSEFLCQTTSMEFLAKYQFDFNACIREGISYLSRAHEDA 180
           FVAHPHNFFVFPRHEIQVDGPSSEFLCQTTS+EFLAKYQFDFNACI EGISYLSRAHEDA
Sbjct: 121 FVAHPHNFFVFPRHEIQVDGPSSEFLCQTTSIEFLAKYQFDFNACIHEGISYLSRAHEDA 180

Query: 181 ARKRLNFAYGDEMSDASSNV--IRDKKRIGIADVLFKERMKNRLHEWRDRLLSERNMEFQ 240
           ARKRLNFA+GDE  DASSNV  IR++KRIGIADVLFKERMKNRLHEWRDRLLSERNME Q
Sbjct: 181 ARKRLNFAHGDETLDASSNVTNIRNQKRIGIADVLFKERMKNRLHEWRDRLLSERNMECQ 240

Query: 241 GQEGSNDYQRQFQTIFFKMRPALTLSDFTSHQIRLIRLKLSVSDIKYFSNSTFCYLFIKS 300
           GQEG+NDYQRQF+TIFFKMRPALTLSDFTSHQ +LI+L                      
Sbjct: 241 GQEGANDYQRQFETIFFKMRPALTLSDFTSHQTKLIQL---------------------- 300

Query: 301 YPCLAACEHMQVIKKHFEDLCFVVVNDGDDNLQQLVVYVDSRDDKELLMQEVNSDQKEAA 360
                      VIKKHFEDLCFVVV+DG+ NLQQLVVYVDSRDDKELLMQEVN D++EAA
Sbjct: 301 -----------VIKKHFEDLCFVVVDDGNANLQQLVVYVDSRDDKELLMQEVNCDRQEAA 360

Query: 361 EMKIQSAIGFRHVIDLLASEKKLIIGHNCFLADTSVFGPDIAHIHTKFIGSLPSTAEEFV 420
           EMKIQSAIGFRHVIDLL+SEKKLIIGHNCFL        DIAHIH KFIGSLP TAEEFV
Sbjct: 361 EMKIQSAIGFRHVIDLLSSEKKLIIGHNCFL--------DIAHIHKKFIGSLPLTAEEFV 420

Query: 421 SSIGKHFPYIMDTKILLNGNSILQRRMKKSSTSLSSAFGLLCPHIAFSSQSNPAFKSGVR 480
           SSIGK FP IMDTKILLNGN ILQ+RMKKSSTSLSSAF LLCPHIAFSSQSNPAF SGV+
Sbjct: 421 SSIGKRFPLIMDTKILLNGNGILQQRMKKSSTSLSSAFALLCPHIAFSSQSNPAFNSGVQ 480

Query: 481 VEVQVDDLRSSNWNSGDKHEAGYDAFMTGCVFAQACNLIGIDFALRSPSENLVDNENLQK 540
           VEVQVDDL SSNWNSGDKHEAGYDAFMTGCVFAQACNL+GIDF L  PSENL DNE LQK
Sbjct: 481 VEVQVDDLSSSNWNSGDKHEAGYDAFMTGCVFAQACNLMGIDFVL--PSENLADNEKLQK 540

Query: 541 HVNHLYLSWASGDIIDLSTGNRMTESLRSNRSKKHQAKISFENIVLIWGFPTHLNAREIK 600
           H+NHLYLSWASGDIIDLSTGNRMTE LRSNRSK+HQ KISFENIVLIWGFP +LNAR+IK
Sbjct: 541 HINHLYLSWASGDIIDLSTGNRMTEPLRSNRSKRHQVKISFENIVLIWGFPANLNARKIK 600

Query: 601 ECISKVFGLTNVVSVYHLDESAVFVQFAKPEMVSEFLDLKENLERHDDPISVLHPLTKLF 660
           ECISKVFGL N+VSVYH+DESAVFVQFAKPEMVSEFLDLKENLERHDDPISVLHPLT+LF
Sbjct: 601 ECISKVFGLANIVSVYHIDESAVFVQFAKPEMVSEFLDLKENLERHDDPISVLHPLTELF 660

Query: 661 EARTTHAAGYEAYKKICSSPISKLLFADQAEAVGINQKTALIKSEAVKSQMHEA------ 720
            A++THAAGYEAYK+ICSSP+ K+LFADQAEAVGINQKT  IK ++VK QMHEA      
Sbjct: 661 GAKSTHAAGYEAYKEICSSPMFKVLFADQAEAVGINQKTMSIKLKSVKGQMHEAANSSLT 710

Query: 721 VEMLNETTEIQGMEMGSNDLSSTNYHSLRSSSM 746
            +ML ETTE+QGME+G+NDL +TNYH LRSSSM
Sbjct: 721 ADMLKETTEVQGMEVGANDLRNTNYHILRSSSM 710

BLAST of CmUC10G186550 vs. ExPASy Swiss-Prot
Match: Q9LG26 (Poly(A)-specific ribonuclease PARN OS=Arabidopsis thaliana OX=3702 GN=PARN PE=1 SV=2)

HSP 1 Score: 734.2 bits (1894), Expect = 1.5e-210
Identity = 379/689 (55.01%), Postives = 478/689 (69.38%), Query Frame = 0

Query: 10  LPRLLCFIRTAAYSSAAVTAPTIASNSSFPLKNVTKLNFEAALSELRTHVRAADFVAVDL 69
           L  L+C   ++A +    T+   ++ ++FPLK+VT+ NFE  L++LR+ V+AADFVA+DL
Sbjct: 9   LRSLVCSFSSSA-AETVTTSTAASATAAFPLKHVTRSNFETTLNDLRSLVKAADFVAIDL 68

Query: 70  EMTGITSAPWRESLEFDRFDIRYLKVKDSAEKFAVVQFGVCPFRWDSAKNSFVAHPHNFF 129
           EMTG+TSAPWR+SLEFDR+D+RYLKVKDSAEKFAVVQFGVCPFRWDS   SFV++PHNFF
Sbjct: 69  EMTGVTSAPWRDSLEFDRYDVRYLKVKDSAEKFAVVQFGVCPFRWDSRTQSFVSYPHNFF 128

Query: 130 VFPRHEIQVDGPSSEFLCQTTSMEFLAKYQFDFNACIREGISYLSRAHEDAARKRLNFAY 189
           VFPR E+  D P+ EFLCQTTSM+FLAKYQFDFN CI EGISYLSR  E+ A KRL   +
Sbjct: 129 VFPRQELTFDPPAHEFLCQTTSMDFLAKYQFDFNTCIHEGISYLSRREEEEASKRLKMLH 188

Query: 190 GDEMSDASSNVIRDKKRIGIADVLFKERMKNRLHEWRDRLLSERNMEFQGQEGSNDYQRQ 249
           G++  D SS    + K + +ADVLF  RM+  L+EWR  LL   N   +    SN   + 
Sbjct: 189 GEDGID-SSGETEELKLVRLADVLFAARMEKLLNEWRSGLLHGGNASSEFPRISNGSNQS 248

Query: 250 FQTIFFKMRPALTLSDFTSHQIRLIRLKLSVSDIKYFSNSTFCYLFIKSYPCLAACEHMQ 309
            +T+F  MRPAL+L  FTSHQ+R++                                   
Sbjct: 249 METVFHHMRPALSLKGFTSHQLRVLN---------------------------------S 308

Query: 310 VIKKHFEDLCFVVVNDGDDNLQQLVVYVDSRDDKELLMQEVNSDQKEAAEMKIQSAIGFR 369
           V++KHF DL ++  ND   + + +VVY DS  DKE LM+E   ++K  AE KIQSAIGFR
Sbjct: 309 VLRKHFGDLVYIHSNDKSSSSRDIVVYTDSDSDKENLMKEAKDERKRLAERKIQSAIGFR 368

Query: 370 HVIDLLASEKKLIIGHNCFLADTSVFGPDIAHIHTKFIGSLPSTAEEFVSSIGKHFPYIM 429
            VIDLLASEKKLI+GHNCFL        DIAH+++KF+G LPSTAE+FV+SI  HFPYI+
Sbjct: 369 QVIDLLASEKKLIVGHNCFL--------DIAHVYSKFVGPLPSTAEKFVASINSHFPYIV 428

Query: 430 DTKILLNGNSILQRRMKKSSTSLSSAFGLLCPHIAFSSQSNPAF-KSGVRVEVQVDDLRS 489
           DTKILLN N +L +RMKKSSTSLSSAF  LCP I FSS+S+ +F +  V ++V++D++R 
Sbjct: 429 DTKILLNVNPMLHQRMKKSSTSLSSAFSSLCPQIEFSSRSSDSFLQQRVNIDVEIDNVRC 488

Query: 490 SNWNSGDKHEAGYDAFMTGCVFAQACNLIGIDFALRSPSENLVDNENLQKHVNHLYLSWA 549
           SNWN+G KHEAGYDAFMTGC+FAQACN +G DF   S  ++   NE L+K++N LYLSW 
Sbjct: 489 SNWNAGGKHEAGYDAFMTGCIFAQACNHLGFDFKQHSQLDDFAQNEKLEKYINRLYLSWT 548

Query: 550 SGDIIDLSTGNRMTESLRSNRSKKHQAKISFENIVLIWGFPTHLNAREIKECISKVFGLT 609
            GDIIDL TG+       SN      +K  +ENIVLIW FP  L AR IKECI K FG  
Sbjct: 549 RGDIIDLRTGH-------SNADNWRVSKFKYENIVLIWNFPRKLKARGIKECICKAFGSA 608

Query: 610 NVVSVYHLDESAVFVQFAKPEMVSEFLDLKENLERHDDPISVLHPLTKLFEARTTHAAGY 669
           +V SVYH+D+SAVFV F   E+V +FL LK  LE  D P+SVLHPL+K+ E   T AA Y
Sbjct: 609 SVTSVYHVDDSAVFVLFKNSELVWDFLALKRQLESSDGPVSVLHPLSKILEGGNTGAADY 647

Query: 670 EAYKKICSSPISKLLFADQAEAVGINQKT 698
           EAYK+ICSS +S+++F+DQAE VG+  +T
Sbjct: 669 EAYKEICSSHVSEVMFSDQAETVGVKSRT 647

BLAST of CmUC10G186550 vs. ExPASy Swiss-Prot
Match: Q8W4C3 (Poly(A)-specific ribonuclease PARN-like OS=Arabidopsis thaliana OX=3702 GN=At3g25430 PE=2 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 5.9e-50
Identity = 166/664 (25.00%), Postives = 293/664 (44.13%), Query Frame = 0

Query: 20  AAYSSAAVTAPTIASNSSFPLKNVTKLNFEAALSELRTHVRAADFVAVDLEMTGITSAPW 79
           ++ S+ A    T+ +   + +K V K NF   L E+RT + ++DF+A+ L+ TG  +A W
Sbjct: 7   SSISATAGNTKTL-NQGRWSVKQVKKSNFHVTLDEIRTSIDSSDFIALSLQNTGSYAAAW 66

Query: 80  RESLEFDRFDIRYLKVKDSAEKFAVVQFGVCPFRWDSAKNSFVAHPHNFFVFPRHEIQVD 139
                 D     YLK K +AE++ ++QF +CPF    +K     HP+NF +FPR E++  
Sbjct: 67  HRVSAIDTPQTSYLKAKYAAERYQILQFALCPFSLQGSK--LTVHPYNFHLFPRDELKCG 126

Query: 140 GPSSEFLCQTTSMEFLAKYQFDFNACIREGISYLSRAHEDAARKRLNFAYGDEMSDASSN 199
            PS  F CQ + +  +A+  FDFN CI EGISYLSRA E A++      +  E    + +
Sbjct: 127 MPSYSFSCQASRLTAMAREGFDFNICIYEGISYLSRAQESASK------FLSENPILADS 186

Query: 200 VIRDKKRIGIADVLFKERMKNRLHEWRDRLLSERNMEFQGQEGSNDYQRQFQTIF----- 259
           V        +AD +F  R+++R+  WR   +   +     + G +D     + +      
Sbjct: 187 VTVSSSPATVADTVFVGRIRSRVKNWRQSCIDSGS-----KTGDDDLVSSLRRLVLGSEQ 246

Query: 260 FKMRPALTLSDFTSHQIRLIRLKLSVSDIKYFSNSTFCYLFIKSYPCLAACEHMQVIKKH 319
           +  R  LT+   +  Q++LI                                 ++++ + 
Sbjct: 247 YGSRLCLTIDVCSERQVQLI---------------------------------LEMLTEF 306

Query: 320 FEDLCFVVVNDGDDNLQQL-VVYVDSRDDKELLMQEVNSDQKEAAEMKIQSAIGFRHVID 379
            +D+  ++V       Q +  V++ S++DK+L  +E+   +KE    + +   GFR V+D
Sbjct: 307 SDDVVPLLVASKSRGTQAVRTVFMSSKEDKDLFKRELKDLEKE----ENRRVRGFREVVD 366

Query: 380 LLASEKKLIIGHNCFLADTSVFGPDIAHIHTKFIGSLPSTAEEFVSSIGKHFPYIMDTKI 439
            ++S +K ++  N        +  D   IH KF+G LPS  ++F SS+   FP ++D   
Sbjct: 367 FISSSQKPVVSQN--------YLSDFTSIHAKFLGPLPSNVDDFSSSLSSAFPNVVDLSQ 426

Query: 440 LLNGNSILQRRMKKSSTSLSSAFGLLCP-HIAFSSQSNPAFKSGVRVEVQVDDLRSSNWN 499
            +   S L   +     ++SS      P  +  ++Q  P         V++D+   S+  
Sbjct: 427 FMKEISPLS-NISNLPAAMSSLNRFFAPVDVEVANQGCP---------VKLDEGHQSH-- 486

Query: 500 SGDKHEAGYDAFMTGCVFAQACNLIGIDFALRSPSENLVDNENLQKHVNHLYLSWASGDI 559
                  G +A M   +FA+ C +       +S    +  NE+ Q        S    + 
Sbjct: 487 -------GQNAVMISQLFAKLCTI------QKSDLSTIQSNEDFQ-----ALASDEHANS 546

Query: 560 IDLSTGNRMTESLRSNRSKKHQAKISFENIVLIWGFPTHLNAREIKECISKVFGL-TNVV 619
           +   + N   E+++     K+  ++S EN+V IWG    + A ++K  + K   +     
Sbjct: 547 VTSCSKNAGDENVKV--WSKNSRRVSSENLVFIWGLGKKMTAAKLKNVLQKSHPVFAREF 572

Query: 620 SVYHLDESAVFVQFAKPEMVSEFLDLKENLERHDDPISVLHPLTKLFEARTTHAAGYEAY 676
            V ++D S+  + F +      FL    N E+ D  +       +   A     AGYE Y
Sbjct: 607 DVKYIDRSSAILVFWESGPSETFLSAVNNEEQLDGSL-------REMVAEGLRGAGYETY 572

BLAST of CmUC10G186550 vs. ExPASy Swiss-Prot
Match: Q7ZU92 (Poly(A)-specific ribonuclease PARN OS=Danio rerio OX=7955 GN=parn PE=1 SV=2)

HSP 1 Score: 141.4 bits (355), Expect = 4.3e-32
Identity = 128/507 (25.25%), Postives = 211/507 (41.62%), Query Frame = 0

Query: 43  VTKLNFEAALSELRTHVRAADFVAVDLEMTGITSAPWRESLE--FDRFDIRYLKVKDSAE 102
           VT+ NF+  L E+   V+ ADF+++D E TGI+  P   +L    D  + RY K++  + 
Sbjct: 3   VTRQNFKEVLPEVCNAVQEADFISIDGEFTGISDGPSVSALTNGLDTPEERYTKLRKHSM 62

Query: 103 KFAVVQFGVCPFRWDSAKNSFVAHPHNFFVFPRHEIQVDGPSSEFLCQTTSMEFLAKYQF 162
            F + QFGVC FR+D  +++++    NF++FP+       P  +F+CQ++S++FLA   F
Sbjct: 63  NFLLFQFGVCTFRYDQNQSTYITKAFNFYIFPK-PFSRTSPDIKFICQSSSIDFLASQGF 122

Query: 163 DFNACIREGISYLSRAHEDAARKRLNFAYGDEMSDASSNVIRDKKRIGIADVLFKERMKN 222
           DFN   R GI YL++  E   R++     G +M+ A           G+ +V   +R   
Sbjct: 123 DFNKVFRSGIPYLNQEEECQLREQYEERRG-QMNGAGPVSYTPPSGTGVCNVPEDQREFI 182

Query: 223 RLHEWRDRLLSERNMEFQGQEGSNDYQRQ--FQTIFFKMRPALTLSDFTSHQIRLIRLKL 282
           R  E +   L +   +    E    +QR+  +QT+  K    L                 
Sbjct: 183 RSVEEKVEALLKNTDQTLDLEPCTGFQRKLIYQTLNSKYSKGL----------------- 242

Query: 283 SVSDIKYFSNSTFCYLFIKSYPCLAACEHMQVIKKHFEDLCFVVVNDGDDNLQQLVVYVD 342
                                              H E L        +   ++  + + 
Sbjct: 243 -----------------------------------HVEAL--------ETEKKERFIQIS 302

Query: 343 SRDDKELLMQEVNSDQKEAAEMKIQSAIGFRHVIDLLASEKKLIIGHNCFLADTSVFGPD 402
             DD+E   +E    Q+E  E  +  A+GF  VI  ++   KL++GHN  L        D
Sbjct: 303 KVDDEERRRREQQKQQREQEE--LNDAVGFSRVIRAISKSGKLVVGHNMLL--------D 362

Query: 403 IAHIHTKFIGSLPSTAEEFVSSIGKHFPYIMDTKILLNGNSILQRRMKKSSTSLSSAFGL 462
           + H   +F G LP   ++F       FP ++DTK++ +       +    +TSL+     
Sbjct: 363 VMHTIHQFCGPLPEELDDFKEVAMTVFPRLLDTKLMASTQPF---KEIIHNTSLAELHKQ 422

Query: 463 LCPHIAFSSQSNPAFKSGVRVEVQVDDLRSSNWNSGDKHEAGYDAFMTGCVFAQACNLIG 522
           L        +  P            + L+S + ++   HEAGYDAF+TG  F    N +G
Sbjct: 423 L--------RQKPFRPPTTECP---EGLQSYDTSTEQLHEAGYDAFITGLCFISMANYLG 422

Query: 523 IDFALRSPSENLVDNENLQKHVNHLYL 546
             F     S     ++ L+   N L+L
Sbjct: 483 -SFLTPPKSHISARSKLLEPFYNKLFL 422

BLAST of CmUC10G186550 vs. ExPASy Swiss-Prot
Match: Q90ZA1 (Poly(A)-specific ribonuclease PARN OS=Xenopus laevis OX=8355 GN=parn PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 3.6e-31
Identity = 127/514 (24.71%), Postives = 211/514 (41.05%), Query Frame = 0

Query: 43  VTKLNFEAALSELRTHVRAADFVAVDLEMTGITSAPWRESLE--FDRFDIRYLKVKDSAE 102
           +T+ NF+  L ++   +  ADF+A+D E +GI+  P   +L   FD  + RY K+K  + 
Sbjct: 3   ITRSNFKDTLPKVYKAIEEADFLAIDGEFSGISDGPSVSTLTNGFDTPEERYTKLKKHSM 62

Query: 103 KFAVVQFGVCPFRWDSAKNSFVAHPHNFFVFPRHEIQVDGPSSEFLCQTTSMEFLAKYQF 162
           +F + QFG+C F +D+ +  ++    NF++FP+     + P  +F+CQ++S++FLA   F
Sbjct: 63  EFLLFQFGLCTFNYDNTEAKYLMKSFNFYIFPK-PFNRNSPDKKFVCQSSSIDFLANQGF 122

Query: 163 DFNACIREGISYLSRAHEDAAR-----KRLNFAYGDEMSDASSNVIRDKKRIGIADVLFK 222
           DFN   R GI YL++  E   R     +R        MS  S N  +    I      F 
Sbjct: 123 DFNKVFRNGIPYLNQEEERVLRDQYEDRRSQSNGASTMSYISPNSSKTPVSIPDEQKGFI 182

Query: 223 ERMKNRLHEWRDRLLSERNMEFQGQEGSNDYQRQ--FQTIFFKMRPALTLSDFTSHQIRL 282
           +++  R+ ++        N+     E    YQR+  +QT+ +K                 
Sbjct: 183 DKVVERVEDFLKNEQKSMNV-----EPCTGYQRKLIYQTLNWK----------------- 242

Query: 283 IRLKLSVSDIKYFSNSTFCYLFIKSYPCLAACEHMQVIKKHFEDLCFVVVNDGDDNLQQL 342
                                    YP     E ++  KK                  + 
Sbjct: 243 -------------------------YPRGIHVETVESEKK------------------ER 302

Query: 343 VVYVDSRDDKELLMQEVNSDQKEAAEMKIQSAIGFRHVIDLLASEKKLIIGHNCFLADTS 402
            + +   D++E    E     KE  E  +  A+GF  +I  ++S  KL++GHN  L    
Sbjct: 303 YIVISKVDEEERKRMEQQKQAKEREE--LDDAVGFSRIIQAISSSGKLVVGHNMLL---- 362

Query: 403 VFGPDIAHIHTKFIGSLPSTAEEFVSSIGKHFPYIMDTKILLNGNSILQRRMKKSSTSLS 462
               D+ H   +F   LP    EF       FP ++DTK++ + N   +     S   L 
Sbjct: 363 ----DVMHTIHQFFCQLPDELNEFKEVTNCVFPRVLDTKLMASTNPFKEIIYNTSLAELE 422

Query: 463 SAFGLLCPHIAFSSQSNPAFKSGVRVEVQVDDLRSSNWNSGDKHEAGYDAFMTGCVFAQA 522
                              FK   +V+   +  +S N  S   HEAGYDA++TG  F   
Sbjct: 423 KRL------------KEAPFKP-PKVD-SAEGFQSYNTASEQLHEAGYDAYITGLCFISM 423

Query: 523 CNLIGIDFALRSPSENLVD--NENLQKHVNHLYL 546
            N +G   +  SP ++ V   ++ ++   N L+L
Sbjct: 483 ANYLG---SFLSPPKDYVSCRSKIVRPFFNKLFL 423

BLAST of CmUC10G186550 vs. ExPASy Swiss-Prot
Match: O95453 (Poly(A)-specific ribonuclease PARN OS=Homo sapiens OX=9606 GN=PARN PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 4.0e-30
Identity = 125/491 (25.46%), Postives = 206/491 (41.96%), Query Frame = 0

Query: 43  VTKLNFEAALSELRTHVRAADFVAVDLEMTGITSAPWRESLE--FDRFDIRYLKVKDSAE 102
           + + NF++ L ++   +  ADF A+D E +GI+  P   +L   FD  + RY K+K  + 
Sbjct: 3   IIRSNFKSNLHKVYQAIEEADFFAIDGEFSGISDGPSVSALTNGFDTPEERYQKLKKHSM 62

Query: 103 KFAVVQFGVCPFRWDSAKNSFVAHPHNFFVFPRHEIQVDGPSSEFLCQTTSMEFLAKYQF 162
            F + QFG+C F++D   + ++    NF+VFP+       P  +F+CQ++S++FLA   F
Sbjct: 63  DFLLFQFGLCTFKYDYTDSKYITKSFNFYVFPK-PFNRSSPDVKFVCQSSSIDFLASQGF 122

Query: 163 DFNACIREGISYLSRAHEDAARKRLNFAYGDEMSDA--SSNVIRDKKRIGIADVLFKERM 222
           DFN   R GI YL++  E    ++L   Y ++ S A  +  +           V   E  
Sbjct: 123 DFNKVFRNGIPYLNQEEE----RQLREQYDEKRSQANGAGALSYVSPNTSKCPVTIPEDQ 182

Query: 223 KNRLHEWRDRLLSERNMEFQGQEGSNDYQRQFQTIFFKMRPALTLSDFTSHQIRLIRLKL 282
           K    ++ D+++ +     Q +E  N                L L   T  Q +LI   L
Sbjct: 183 K----KFIDQVVEKIEDLLQSEENKN----------------LDLEPCTGFQRKLIYQTL 242

Query: 283 SVSDIKYFSNSTFCYLFIKSYPCLAACEHMQVIKKHFEDLCFVVVNDGDDNLQQLVVYVD 342
           S                   YP     E ++  KK                  +  + + 
Sbjct: 243 S-----------------WKYPKGIHVETLETEKK------------------ERYIVIS 302

Query: 343 SRDDKELLMQEVNSDQKEAAEMKIQSAIGFRHVIDLLASEKKLIIGHNCFLADTSVFGPD 402
             D++E   +E     KE  E  +  A+GF  VI  +A+  KL+IGHN  L        D
Sbjct: 303 KVDEEERKRREQQKHAKEQEE--LNDAVGFSRVIHAIANSGKLVIGHNMLL--------D 362

Query: 403 IAHIHTKFIGSLPSTAEEFVSSIGKHFPYIMDTKI---------LLNGNSI--LQRRMKK 462
           + H   +F   LP+   EF       FP ++DTK+         ++N  S+  L++R+K+
Sbjct: 363 VMHTVHQFYCPLPADLSEFKEMTTCVFPRLLDTKLMASTQPFKDIINNTSLAELEKRLKE 398

Query: 463 SSTSLSSAFGLLCPHIAFSSQSNPAFKSGVRVEVQVDDLRSSNWNSGDKHEAGYDAFMTG 519
           +  +         P    S++  P++ +                 S   HEAGYDA++TG
Sbjct: 423 TPFN---------PPKVESAEGFPSYDTA----------------SEQLHEAGYDAYITG 398

BLAST of CmUC10G186550 vs. ExPASy TrEMBL
Match: A0A6J1EWU6 (poly(A)-specific ribonuclease PARN isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111438751 PE=3 SV=1)

HSP 1 Score: 1213.0 bits (3137), Expect = 0.0e+00
Identity = 628/753 (83.40%), Postives = 664/753 (88.18%), Query Frame = 0

Query: 1   MDPSRSLSALPRLLCFIRTAAYSSAAVTAPTIASNSSFPLKNVTKLNFEAALSELRTHVR 60
           M+ SR L A  RLLCFIRTA YSSAAVT P  ASNSSFPLKNVTK NFEA LSELR+HVR
Sbjct: 1   MNLSRPLWAPTRLLCFIRTATYSSAAVTTPATASNSSFPLKNVTKSNFEAVLSELRSHVR 60

Query: 61  AADFVAVDLEMTGITSAPWRESLEFDRFDIRYLKVKDSAEKFAVVQFGVCPFRWDSAKNS 120
           AADFVA+DLEMTGITSAPWRES EFDRFD+RYLKVKDSAEKFAVVQFGVCPFRWDSAKNS
Sbjct: 61  AADFVAIDLEMTGITSAPWRESFEFDRFDVRYLKVKDSAEKFAVVQFGVCPFRWDSAKNS 120

Query: 121 FVAHPHNFFVFPRHEIQVDGPSSEFLCQTTSMEFLAKYQFDFNACIREGISYLSRAHEDA 180
           FVAHPHNFFVFPRHEI VDGPSSEFLCQTTS+EFLAKYQFDFNACI EGISYLSRAHEDA
Sbjct: 121 FVAHPHNFFVFPRHEILVDGPSSEFLCQTTSIEFLAKYQFDFNACIHEGISYLSRAHEDA 180

Query: 181 ARKRLNFAYGDEMSDASSNV--IRDKKRIGIADVLFKERMKNRLHEWRDRLLSERNMEFQ 240
           ARKRLNFA+GDE SDASSNV  IRD+KRIGIADVLFKERMKNRLHEWRDRLLSERNME Q
Sbjct: 181 ARKRLNFAHGDETSDASSNVTNIRDQKRIGIADVLFKERMKNRLHEWRDRLLSERNMECQ 240

Query: 241 GQEGSNDYQRQFQTIFFKMRPALTLSDFTSHQIRLIRLKLSVSDIKYFSNSTFCYLFIKS 300
           GQEGSNDYQRQF+TIFFKMRPALTLSDFTSHQ +LI+L                      
Sbjct: 241 GQEGSNDYQRQFETIFFKMRPALTLSDFTSHQTKLIQL---------------------- 300

Query: 301 YPCLAACEHMQVIKKHFEDLCFVVVNDGDDNLQQLVVYVDSRDDKELLMQEVNSDQKEAA 360
                      VIKKHFEDLCFVVV+DGD NLQQLVVYVDSRDDKELLMQEVNSD++EAA
Sbjct: 301 -----------VIKKHFEDLCFVVVDDGDANLQQLVVYVDSRDDKELLMQEVNSDRQEAA 360

Query: 361 EMKIQSAIGFRHVIDLLASEKKLIIGHNCFLADTSVFGPDIAHIHTKFIGSLPSTAEEFV 420
           EMKIQSAIGFRHVIDLL+SEKKLIIGHNCFL        DIAHIH KFIGSLPSTAEEFV
Sbjct: 361 EMKIQSAIGFRHVIDLLSSEKKLIIGHNCFL--------DIAHIHKKFIGSLPSTAEEFV 420

Query: 421 SSIGKHFPYIMDTKILLNGNSILQRRMKKSSTSLSSAFGLLCPHIAFSSQSNPAFKSGVR 480
           SSIGKHFP+IMDTKILLNGN ILQ+RMKKSSTSLSSAF LLCPHIAFSSQSNPAF SGV+
Sbjct: 421 SSIGKHFPFIMDTKILLNGNGILQQRMKKSSTSLSSAFALLCPHIAFSSQSNPAFSSGVQ 480

Query: 481 VEVQVDDLRSSNWNSGDKHEAGYDAFMTGCVFAQACNLIGIDFALRSPSENLVDNENLQK 540
           VEVQVDDL SSNWNSGDKHEAGYDAFMTGCVFAQACNL+GIDF L  PSENL DNE LQK
Sbjct: 481 VEVQVDDLSSSNWNSGDKHEAGYDAFMTGCVFAQACNLMGIDFVL--PSENLADNEKLQK 540

Query: 541 HVNHLYLSWASGDIIDLSTGNRMTESLRSNRSKKHQAKISFENIVLIWGFPTHLNAREIK 600
           H+NHLYLSWASGDIIDLSTGNRMTE LRSNRSKKHQAKISFENIVLIWGFP +LNAR+IK
Sbjct: 541 HINHLYLSWASGDIIDLSTGNRMTEPLRSNRSKKHQAKISFENIVLIWGFPANLNARKIK 600

Query: 601 ECISKVFGLTNVVSVYHLDESAVFVQFAKPEMVSEFLDLKENLERHDDPISVLHPLTKLF 660
           ECISKVFGL N+VSVYH+DESAVFVQFAKPEMVSEFLDLK+NLERHDDPISVLHPLT+LF
Sbjct: 601 ECISKVFGLANIVSVYHIDESAVFVQFAKPEMVSEFLDLKKNLERHDDPISVLHPLTELF 660

Query: 661 EARTTHAAGYEAYKKICSSPISKLLFADQAEAVGINQKTALIKSEAVKSQMHEA------ 720
            A+ T+AAGYEAYK+ICSSP+ K+LFADQAEAVGINQKT  IK E+VKSQMHEA      
Sbjct: 661 GAKGTNAAGYEAYKEICSSPMFKVLFADQAEAVGINQKTTSIKPESVKSQMHEAANNSLT 710

Query: 721 VEMLNETTEIQGMEMGSNDLSSTNYHSLRSSSM 746
            +MLNETTE+QGME+G+NDL +TNYH LRSSSM
Sbjct: 721 ADMLNETTEVQGMEVGANDLRNTNYHILRSSSM 710

BLAST of CmUC10G186550 vs. ExPASy TrEMBL
Match: A0A6J1L1G4 (poly(A)-specific ribonuclease PARN isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111500250 PE=3 SV=1)

HSP 1 Score: 1197.6 bits (3097), Expect = 0.0e+00
Identity = 620/753 (82.34%), Postives = 659/753 (87.52%), Query Frame = 0

Query: 1   MDPSRSLSALPRLLCFIRTAAYSSAAVTAPTIASNSSFPLKNVTKLNFEAALSELRTHVR 60
           M+ SR L A  RLLCFIRTA YSSAAVTAP  ASNSSFPLKNVTK NFEA LSELR+HVR
Sbjct: 1   MNLSRPLWAPTRLLCFIRTATYSSAAVTAPATASNSSFPLKNVTKSNFEAVLSELRSHVR 60

Query: 61  AADFVAVDLEMTGITSAPWRESLEFDRFDIRYLKVKDSAEKFAVVQFGVCPFRWDSAKNS 120
           AADFVA+DLEMTGITSAPWRES EFDRFD+RYLKVKDSAEKFAVVQFGVCPFRWDSAKNS
Sbjct: 61  AADFVAIDLEMTGITSAPWRESFEFDRFDVRYLKVKDSAEKFAVVQFGVCPFRWDSAKNS 120

Query: 121 FVAHPHNFFVFPRHEIQVDGPSSEFLCQTTSMEFLAKYQFDFNACIREGISYLSRAHEDA 180
           FVAHPHNFFVFPRHEIQVDGPSSEFLCQTTS+EFLAKYQFDFNACI EGISYLSRAHEDA
Sbjct: 121 FVAHPHNFFVFPRHEIQVDGPSSEFLCQTTSIEFLAKYQFDFNACIHEGISYLSRAHEDA 180

Query: 181 ARKRLNFAYGDEMSDASSNV--IRDKKRIGIADVLFKERMKNRLHEWRDRLLSERNMEFQ 240
           ARKRLNFA+GDE  DASSNV  IR++KRIGIADVLFKERMKNRLHEWRDRLLSERNME Q
Sbjct: 181 ARKRLNFAHGDETLDASSNVTNIRNQKRIGIADVLFKERMKNRLHEWRDRLLSERNMECQ 240

Query: 241 GQEGSNDYQRQFQTIFFKMRPALTLSDFTSHQIRLIRLKLSVSDIKYFSNSTFCYLFIKS 300
           GQEG+NDYQRQF+TIFFKMRPALTLSDFTSHQ +LI+L                      
Sbjct: 241 GQEGANDYQRQFETIFFKMRPALTLSDFTSHQTKLIQL---------------------- 300

Query: 301 YPCLAACEHMQVIKKHFEDLCFVVVNDGDDNLQQLVVYVDSRDDKELLMQEVNSDQKEAA 360
                      VIKKHFEDLCFVVV+DG+ NLQQLVVYVDSRDDKELLMQEVN D++EAA
Sbjct: 301 -----------VIKKHFEDLCFVVVDDGNANLQQLVVYVDSRDDKELLMQEVNCDRQEAA 360

Query: 361 EMKIQSAIGFRHVIDLLASEKKLIIGHNCFLADTSVFGPDIAHIHTKFIGSLPSTAEEFV 420
           EMKIQSAIGFRHVIDLL+SEKKLIIGHNCFL        DIAHIH KFIGSLP TAEEFV
Sbjct: 361 EMKIQSAIGFRHVIDLLSSEKKLIIGHNCFL--------DIAHIHKKFIGSLPLTAEEFV 420

Query: 421 SSIGKHFPYIMDTKILLNGNSILQRRMKKSSTSLSSAFGLLCPHIAFSSQSNPAFKSGVR 480
           SSIGK FP IMDTKILLNGN ILQ+RMKKSSTSLSSAF LLCPHIAFSSQSNPAF SGV+
Sbjct: 421 SSIGKRFPLIMDTKILLNGNGILQQRMKKSSTSLSSAFALLCPHIAFSSQSNPAFNSGVQ 480

Query: 481 VEVQVDDLRSSNWNSGDKHEAGYDAFMTGCVFAQACNLIGIDFALRSPSENLVDNENLQK 540
           VEVQVDDL SSNWNSGDKHEAGYDAFMTGCVFAQACNL+GIDF L  PSENL DNE LQK
Sbjct: 481 VEVQVDDLSSSNWNSGDKHEAGYDAFMTGCVFAQACNLMGIDFVL--PSENLADNEKLQK 540

Query: 541 HVNHLYLSWASGDIIDLSTGNRMTESLRSNRSKKHQAKISFENIVLIWGFPTHLNAREIK 600
           H+NHLYLSWASGDIIDLSTGNRMTE LRSNRSK+HQ KISFENIVLIWGFP +LNAR+IK
Sbjct: 541 HINHLYLSWASGDIIDLSTGNRMTEPLRSNRSKRHQVKISFENIVLIWGFPANLNARKIK 600

Query: 601 ECISKVFGLTNVVSVYHLDESAVFVQFAKPEMVSEFLDLKENLERHDDPISVLHPLTKLF 660
           ECISKVFGL N+VSVYH+DESAVFVQFAKPEMVSEFLDLKENLERHDDPISVLHPLT+LF
Sbjct: 601 ECISKVFGLANIVSVYHIDESAVFVQFAKPEMVSEFLDLKENLERHDDPISVLHPLTELF 660

Query: 661 EARTTHAAGYEAYKKICSSPISKLLFADQAEAVGINQKTALIKSEAVKSQMHEA------ 720
            A++THAAGYEAYK+ICSSP+ K+LFADQAEAVGINQKT  IK ++VK QMHEA      
Sbjct: 661 GAKSTHAAGYEAYKEICSSPMFKVLFADQAEAVGINQKTMSIKLKSVKGQMHEAANSSLT 710

Query: 721 VEMLNETTEIQGMEMGSNDLSSTNYHSLRSSSM 746
            +ML ETTE+QGME+G+NDL +TNYH LRSSSM
Sbjct: 721 ADMLKETTEVQGMEVGANDLRNTNYHILRSSSM 710

BLAST of CmUC10G186550 vs. ExPASy TrEMBL
Match: A0A0A0L4V6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G048430 PE=3 SV=1)

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 588/748 (78.61%), Postives = 641/748 (85.70%), Query Frame = 0

Query: 1   MDPSRSLSALP-RLLCFIRTAAYSSAAVTAPTIASNSSFPLKNVTKLNFEAALSELRTHV 60
           M+PS  L AL  RL CFIRT  YSSAAVTAP+ ASNSSFPLKNVTK NFEAALSELR+HV
Sbjct: 1   MNPSHPLWALQRRLFCFIRTTNYSSAAVTAPSTASNSSFPLKNVTKSNFEAALSELRSHV 60

Query: 61  RAADFVAVDLEMTGITSAPWRESLEFDRFDIRYLKVKDSAEKFAVVQFGVCPFRWDSAKN 120
           RAADFVA+DLEMTGITSAPWR+ LEFDRFD+RYL VKDSAEKFAV+QFG+CPFRWDSAKN
Sbjct: 61  RAADFVAIDLEMTGITSAPWRDLLEFDRFDVRYLNVKDSAEKFAVLQFGICPFRWDSAKN 120

Query: 121 SFVAHPHNFFVFPRHEIQVDGPSSEFLCQTTSMEFLAKYQFDFNACIREGISYLSRAHED 180
           SFVAHPHNFF+FPRHEI+VDGPSSEFLCQTTSMEFLAKYQFDFNACI EGISYLSRAHED
Sbjct: 121 SFVAHPHNFFIFPRHEIEVDGPSSEFLCQTTSMEFLAKYQFDFNACIYEGISYLSRAHED 180

Query: 181 AARKRLNFAYGDEMSDASSNV--IRDKKRIGIADVLFKERMKNRLHEWRDRLLSERNMEF 240
           AARKRLNFAYGDEM DASSNV  + DKKR+GI DVLFKERMKNRLHEWRDRLLSERNMEF
Sbjct: 181 AARKRLNFAYGDEMLDASSNVTNVMDKKRVGIPDVLFKERMKNRLHEWRDRLLSERNMEF 240

Query: 241 QGQEGSNDYQRQFQTIFFKMRPALTLSDFTSHQIRLIRLKLSVSDIKYFSNSTFCYLFIK 300
           QGQEGSND++RQFQTIFFKMRPALTLSDFTSHQI+LI+L                     
Sbjct: 241 QGQEGSNDHKRQFQTIFFKMRPALTLSDFTSHQIKLIQL--------------------- 300

Query: 301 SYPCLAACEHMQVIKKHFEDLCFVVVNDGDDNLQQLVVYVDSRDDKELLMQEVNSDQKEA 360
                       VIKKHFEDLCFVVVNDG+ +LQQLVV+VDSRDDKELLMQEVN DQKE 
Sbjct: 301 ------------VIKKHFEDLCFVVVNDGNADLQQLVVFVDSRDDKELLMQEVNRDQKEV 360

Query: 361 AEMKIQSAIGFRHVIDLLASEKKLIIGHNCFLADTSVFGPDIAHIHTKFIGSLPSTAEEF 420
            EMKIQSAIGFRHVIDLL+SEKKLI+GHNCFL        D+ HIH KFIGSLPST EEF
Sbjct: 361 NEMKIQSAIGFRHVIDLLSSEKKLIVGHNCFL--------DMLHIHRKFIGSLPSTTEEF 420

Query: 421 VSSIGKHFPYIMDTKILLNGNSILQRRMKKSSTSLSSAFGLLCPHIAFSSQSNPAFKSGV 480
           VSS+GKHFPYIMDTKILLNGN+IL RRM KS+TSLSSAFGLLCPHIAFSSQ   A+ SGV
Sbjct: 421 VSSLGKHFPYIMDTKILLNGNNILIRRMNKSNTSLSSAFGLLCPHIAFSSQRVSAWNSGV 480

Query: 481 RVEVQVDDLRSSNWNSGDKHEAGYDAFMTGCVFAQACNLIGIDFALRSPSENLVDNENLQ 540
           +VEVQVDDLRSS WNSGD HEAGYDAFMTGCVFAQAC+L+GIDF  RS SENL+ NE LQ
Sbjct: 481 QVEVQVDDLRSSKWNSGDTHEAGYDAFMTGCVFAQACDLMGIDFVDRSTSENLLGNEILQ 540

Query: 541 KHVNHLYLSWASGDIIDLSTGNRMTESLRSNRSKKHQAKISFENIVLIWGFPTHLNAREI 600
           KH+N LYLSWA GDIIDLSTGN++TES     ++KH+AK+SF+NIVLIWGFPT+L AR+I
Sbjct: 541 KHINRLYLSWARGDIIDLSTGNQVTESF---TTRKHRAKVSFQNIVLIWGFPTNLTARKI 600

Query: 601 KECISKVFGLTNVVSVYHLDESAVFVQFAKPEMVSEFLDLKENLERHDDPISVLHPLTKL 660
           K CISK FG TNV SV HLDESAVFVQF KPEMVSEFLDLKE LE+ DDP+SVLHPLT+L
Sbjct: 601 KACISKAFGQTNVSSVNHLDESAVFVQFTKPEMVSEFLDLKEKLEKRDDPLSVLHPLTEL 660

Query: 661 FEARTTHAAGYEAYKKICSSPISKLLFADQAEAVGINQKTALIKSEAVKSQMHEAVEMLN 720
           F+ RTTHAAGYEAYKKICSSP+SK LF+DQAEAVGIN+KT  IKSE  +S+MH+AV    
Sbjct: 661 FDVRTTHAAGYEAYKKICSSPMSKHLFSDQAEAVGINRKTTSIKSEEKRSRMHQAV---- 700

Query: 721 ETTEIQGMEMGSNDLSSTNYHSLRSSSM 746
           ETTEIQG E+ +NDLS+TNYHSLRSSSM
Sbjct: 721 ETTEIQGTEVEANDLSNTNYHSLRSSSM 700

BLAST of CmUC10G186550 vs. ExPASy TrEMBL
Match: A0A6J1CSK6 (poly(A)-specific ribonuclease PARN isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013834 PE=3 SV=1)

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 593/754 (78.65%), Postives = 639/754 (84.75%), Query Frame = 0

Query: 1   MDPSRSLSALPRLLCFIRTAAYSSAAVTAPTIASNSSFPLKNVTKLNFEAALSELRTHVR 60
           M   R L ALPRLLCFIRTA YSSAAVTAP  A N SFPLKNVTK NFEA ++ELR HVR
Sbjct: 1   MKTPRPLWALPRLLCFIRTATYSSAAVTAPATAVNPSFPLKNVTKSNFEAVVAELRNHVR 60

Query: 61  AADFVAVDLEMTGITSAPWRESLEFDRFDIRYLKVKDSAEKFAVVQFGVCPFRWDSAKNS 120
           AADFVA+DLEMTGITSAPWRES EFDRFD+RYLKVKDSAEKFAVVQFGVCPFRWDS KNS
Sbjct: 61  AADFVAIDLEMTGITSAPWRESFEFDRFDVRYLKVKDSAEKFAVVQFGVCPFRWDSTKNS 120

Query: 121 FVAHPHNFFVFPRHEIQVDGPSSEFLCQTTSMEFLAKYQFDFNACIREGISYLSRAHEDA 180
           F+AHPHNFFVFPR EIQVDGPS EFLCQTTS+EFLAKYQFDFNACI EGISYLSRA E A
Sbjct: 121 FIAHPHNFFVFPRQEIQVDGPSPEFLCQTTSVEFLAKYQFDFNACIHEGISYLSRAQEYA 180

Query: 181 ARKRLNFAYGDEMSDASSNV--IRDKKRIGIADVLFKERMKNRLHEWRDRLLSERNMEFQ 240
           ARKRL+FAYGDEMSDASSNV  I+D+KR+ I+DVLFKERMKNRLHEWRDRLLSERN+EFQ
Sbjct: 181 ARKRLDFAYGDEMSDASSNVTDIKDEKRVSISDVLFKERMKNRLHEWRDRLLSERNVEFQ 240

Query: 241 GQEGSNDYQRQFQTIFFKMRPALTLSDFTSHQIRLIRLKLSVSDIKYFSNSTFCYLFIKS 300
           GQE S  YQR FQTIFFKMRPAL L+DFTSHQ RLI+L                      
Sbjct: 241 GQECS--YQRPFQTIFFKMRPALCLNDFTSHQTRLIQL---------------------- 300

Query: 301 YPCLAACEHMQVIKKHFEDLCFVVVNDGDDNLQQLVVYVDSRDDKELLMQEVNSDQKEAA 360
                      VIKKHF DLC+V V++G+ NLQQLVVYVD RDDKELLM+EVNS++++AA
Sbjct: 301 -----------VIKKHFTDLCYVHVDNGNANLQQLVVYVDYRDDKELLMKEVNSERQKAA 360

Query: 361 EMKIQSAIGFRHVIDLLASEKKLIIGHNCFLADTSVFGPDIAHIHTKFIGSLPSTAEEFV 420
           EMKIQSAIGFRHVIDLL+ EKKLI+GHNCFL        DIAHIH+KFIGSLPSTAEEFV
Sbjct: 361 EMKIQSAIGFRHVIDLLSLEKKLIVGHNCFL--------DIAHIHSKFIGSLPSTAEEFV 420

Query: 421 SSIGKHFPYIMDTKILLNGNSILQRRMKKSSTSLSSAFGLLCPHIAFS--SQSNPAFKSG 480
           SSIGKHFPYIMDTKILLNGNSILQ+RMKKSSTSLSSA G+LCPHIAFS  S+S+PAF SG
Sbjct: 421 SSIGKHFPYIMDTKILLNGNSILQQRMKKSSTSLSSALGVLCPHIAFSSHSKSSPAFNSG 480

Query: 481 VRVEVQVDDLRSSNWNSGDKHEAGYDAFMTGCVFAQACNLIGIDFALRSPSENLVDNENL 540
           V+VEVQVDDLRSSNWNSGDKHEAGYDAFMTGCVFAQACNLIGIDF   SPSE L DNE  
Sbjct: 481 VQVEVQVDDLRSSNWNSGDKHEAGYDAFMTGCVFAQACNLIGIDFVRSSPSETLADNEKF 540

Query: 541 QKHVNHLYLSWASGDIIDLSTGNRMTESLRSNRSKKHQAKISFENIVLIWGFPTHLNARE 600
           Q HVNHLYLSW SGDIIDLSTGNR TESLRSNRSKKHQAKISFENIVLIWGFPT++NAR+
Sbjct: 541 QNHVNHLYLSWXSGDIIDLSTGNRRTESLRSNRSKKHQAKISFENIVLIWGFPTNINARK 600

Query: 601 IKECISKVFGLTNVVSVYHLDESAVFVQFAKPEMVSEFLDLKENLERHDDPISVLHPLTK 660
           IK+CISKVFG TNVVSVYHLDESAVFVQFAK EMVSEFL LKENLERH+DP+SVLHPL +
Sbjct: 601 IKQCISKVFGPTNVVSVYHLDESAVFVQFAKLEMVSEFLMLKENLERHNDPVSVLHPLYE 660

Query: 661 LFEARTTHAAGYEAYKKICSSPISKLLFADQAEAVGINQKTALIKSEAVKSQMHEA---- 720
           LFEAR THAA YEAYK+ICSSPI KLLFADQAEAVG N+KT  IK E VKSQMHEA    
Sbjct: 661 LFEARNTHAACYEAYKEICSSPICKLLFADQAEAVGFNRKTKSIKPETVKSQMHEAANGL 711

Query: 721 -VEMLNETTEIQGMEMGSNDLSSTNYHSLRSSSM 746
             +ML+  TEIQ  E+G+ND+S TNY+ L  SSM
Sbjct: 721 TADMLDGNTEIQETEIGANDISCTNYNVLNLSSM 711

BLAST of CmUC10G186550 vs. ExPASy TrEMBL
Match: A0A5D3CQS8 (Poly(A)-specific ribonuclease PARN OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold488G00310 PE=3 SV=1)

HSP 1 Score: 1125.9 bits (2911), Expect = 0.0e+00
Identity = 585/747 (78.31%), Postives = 638/747 (85.41%), Query Frame = 0

Query: 1   MDPSRSLSALPRLLCFIRTAAYSSAAVTAPTIASNSSFPLKNVTKLNFEAALSELRTHVR 60
           M PS  L A  RL CFIRT  YSSAAVTAP  ASNSSFPLKNVTK NFEAALSELR+HVR
Sbjct: 1   MHPSHPLWA-RRLFCFIRTTNYSSAAVTAPATASNSSFPLKNVTKSNFEAALSELRSHVR 60

Query: 61  AADFVAVDLEMTGITSAPWRESLEFDRFDIRYLKVKDSAEKFAVVQFGVCPFRWDSAKNS 120
           AADFVAVDLEMTGITSAPWR+ LEFDRFD+RYL VKDSAEKFAVVQFGVCPFRWDSAK S
Sbjct: 61  AADFVAVDLEMTGITSAPWRDLLEFDRFDVRYLNVKDSAEKFAVVQFGVCPFRWDSAKKS 120

Query: 121 FVAHPHNFFVFPRHEIQVDGPSSEFLCQTTSMEFLAKYQFDFNACIREGISYLSRAHEDA 180
           FVAHPHNFF+FPRHEI+VDGPSSEFLC+TTS+EFLAKYQFDFNACI EGISYLSRAHEDA
Sbjct: 121 FVAHPHNFFIFPRHEIEVDGPSSEFLCRTTSLEFLAKYQFDFNACIYEGISYLSRAHEDA 180

Query: 181 ARKRLNFAYGDEMSDASSNV--IRDKKRIGIADVLFKERMKNRLHEWRDRLLSERNMEFQ 240
           ARKRLNFAYGDEM DASSNV  +RDKKR+GIADVLF+ERMKNRLHEWRDRLLSERNMEFQ
Sbjct: 181 ARKRLNFAYGDEMLDASSNVTNVRDKKRVGIADVLFRERMKNRLHEWRDRLLSERNMEFQ 240

Query: 241 GQEGSNDYQRQFQTIFFKMRPALTLSDFTSHQIRLIRLKLSVSDIKYFSNSTFCYLFIKS 300
           GQEGSND++RQFQTIFFK RPALTLSDFTSHQI+LI+L                      
Sbjct: 241 GQEGSNDHKRQFQTIFFKTRPALTLSDFTSHQIKLIQL---------------------- 300

Query: 301 YPCLAACEHMQVIKKHFEDLCFVVVNDGDDNLQQLVVYVDSRDDKELLMQEVNSDQKEAA 360
                      VIKKHFEDLCFVVVNDG+ +LQQLVVYVDSRDDKELLMQEVN DQ +AA
Sbjct: 301 -----------VIKKHFEDLCFVVVNDGNADLQQLVVYVDSRDDKELLMQEVNRDQNKAA 360

Query: 361 EMKIQSAIGFRHVIDLLASEKKLIIGHNCFLADTSVFGPDIAHIHTKFIGSLPSTAEEFV 420
           E KIQSAIGFRHVIDLL+SEKKLI+GHNCFL        D+ HIH KFIGSLPSTAEEFV
Sbjct: 361 ETKIQSAIGFRHVIDLLSSEKKLIVGHNCFL--------DMLHIHRKFIGSLPSTAEEFV 420

Query: 421 SSIGKHFPYIMDTKILLNGNSILQRRMKKSSTSLSSAFGLLCPHIAFSSQSNPAFKSGVR 480
           SS GKHFPY+MDTKILLN N IL+RRM KS+TSLSSAFG LCPHIAFSS    A   GV+
Sbjct: 421 SSFGKHFPYVMDTKILLNDNYILRRRMNKSNTSLSSAFGSLCPHIAFSSLRVNALNDGVQ 480

Query: 481 VEVQVDDLRSSNWNSGDKHEAGYDAFMTGCVFAQACNLIGIDFALRSPSENLVDNENLQK 540
           VEVQVDDLRSS WNSGDKHEAGYDAFMTGCVFAQACNL+GIDF   S S+NLVDNE LQK
Sbjct: 481 VEVQVDDLRSSKWNSGDKHEAGYDAFMTGCVFAQACNLMGIDFVDCSTSKNLVDNEILQK 540

Query: 541 HVNHLYLSWASGDIIDLSTGNRMTESLRSNRSKKHQAKISFENIVLIWGFPTHLNAREIK 600
           H+N LYL+WASGDIIDLSTGN++TES    +++KH+ ++SFENIVLIWGF T+L AR+IK
Sbjct: 541 HINRLYLNWASGDIIDLSTGNQVTESF---KTRKHRVRVSFENIVLIWGFSTNLTARKIK 600

Query: 601 ECISKVFGLTNVVSVYHLDESAVFVQFAKPEMVSEFLDLKENLERHDDPISVLHPLTKLF 660
            CISKVFG  NV SV HLDESAVFVQF KPEMVSEFL+LKE LE+ DDPISVLHPLT+LF
Sbjct: 601 ACISKVFGQANVSSVNHLDESAVFVQFTKPEMVSEFLNLKEKLEKRDDPISVLHPLTELF 660

Query: 661 EARTTHAAGYEAYKKICSSPISKLLFADQAEAVGINQKTALIKSEAVKSQMHEAVEMLNE 720
           +AR THAAGYEAYKKICSSP+SKLLF+DQAEAVGIN+KT LIKSEA +S++ +AV+ML+E
Sbjct: 661 DARATHAAGYEAYKKICSSPMSKLLFSDQAEAVGINRKTMLIKSEAKRSKIDQAVDMLDE 702

Query: 721 TTEIQGMEMGSNDLSSTNYHSLRSSSM 746
           TTEIQG E  +N+LSSTNY SLRSSSM
Sbjct: 721 TTEIQGTEGEANNLSSTNYQSLRSSSM 702

BLAST of CmUC10G186550 vs. TAIR 10
Match: AT1G55870.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 734.2 bits (1894), Expect = 1.1e-211
Identity = 379/689 (55.01%), Postives = 478/689 (69.38%), Query Frame = 0

Query: 10  LPRLLCFIRTAAYSSAAVTAPTIASNSSFPLKNVTKLNFEAALSELRTHVRAADFVAVDL 69
           L  L+C   ++A +    T+   ++ ++FPLK+VT+ NFE  L++LR+ V+AADFVA+DL
Sbjct: 9   LRSLVCSFSSSA-AETVTTSTAASATAAFPLKHVTRSNFETTLNDLRSLVKAADFVAIDL 68

Query: 70  EMTGITSAPWRESLEFDRFDIRYLKVKDSAEKFAVVQFGVCPFRWDSAKNSFVAHPHNFF 129
           EMTG+TSAPWR+SLEFDR+D+RYLKVKDSAEKFAVVQFGVCPFRWDS   SFV++PHNFF
Sbjct: 69  EMTGVTSAPWRDSLEFDRYDVRYLKVKDSAEKFAVVQFGVCPFRWDSRTQSFVSYPHNFF 128

Query: 130 VFPRHEIQVDGPSSEFLCQTTSMEFLAKYQFDFNACIREGISYLSRAHEDAARKRLNFAY 189
           VFPR E+  D P+ EFLCQTTSM+FLAKYQFDFN CI EGISYLSR  E+ A KRL   +
Sbjct: 129 VFPRQELTFDPPAHEFLCQTTSMDFLAKYQFDFNTCIHEGISYLSRREEEEASKRLKMLH 188

Query: 190 GDEMSDASSNVIRDKKRIGIADVLFKERMKNRLHEWRDRLLSERNMEFQGQEGSNDYQRQ 249
           G++  D SS    + K + +ADVLF  RM+  L+EWR  LL   N   +    SN   + 
Sbjct: 189 GEDGID-SSGETEELKLVRLADVLFAARMEKLLNEWRSGLLHGGNASSEFPRISNGSNQS 248

Query: 250 FQTIFFKMRPALTLSDFTSHQIRLIRLKLSVSDIKYFSNSTFCYLFIKSYPCLAACEHMQ 309
            +T+F  MRPAL+L  FTSHQ+R++                                   
Sbjct: 249 METVFHHMRPALSLKGFTSHQLRVLN---------------------------------S 308

Query: 310 VIKKHFEDLCFVVVNDGDDNLQQLVVYVDSRDDKELLMQEVNSDQKEAAEMKIQSAIGFR 369
           V++KHF DL ++  ND   + + +VVY DS  DKE LM+E   ++K  AE KIQSAIGFR
Sbjct: 309 VLRKHFGDLVYIHSNDKSSSSRDIVVYTDSDSDKENLMKEAKDERKRLAERKIQSAIGFR 368

Query: 370 HVIDLLASEKKLIIGHNCFLADTSVFGPDIAHIHTKFIGSLPSTAEEFVSSIGKHFPYIM 429
            VIDLLASEKKLI+GHNCFL        DIAH+++KF+G LPSTAE+FV+SI  HFPYI+
Sbjct: 369 QVIDLLASEKKLIVGHNCFL--------DIAHVYSKFVGPLPSTAEKFVASINSHFPYIV 428

Query: 430 DTKILLNGNSILQRRMKKSSTSLSSAFGLLCPHIAFSSQSNPAF-KSGVRVEVQVDDLRS 489
           DTKILLN N +L +RMKKSSTSLSSAF  LCP I FSS+S+ +F +  V ++V++D++R 
Sbjct: 429 DTKILLNVNPMLHQRMKKSSTSLSSAFSSLCPQIEFSSRSSDSFLQQRVNIDVEIDNVRC 488

Query: 490 SNWNSGDKHEAGYDAFMTGCVFAQACNLIGIDFALRSPSENLVDNENLQKHVNHLYLSWA 549
           SNWN+G KHEAGYDAFMTGC+FAQACN +G DF   S  ++   NE L+K++N LYLSW 
Sbjct: 489 SNWNAGGKHEAGYDAFMTGCIFAQACNHLGFDFKQHSQLDDFAQNEKLEKYINRLYLSWT 548

Query: 550 SGDIIDLSTGNRMTESLRSNRSKKHQAKISFENIVLIWGFPTHLNAREIKECISKVFGLT 609
            GDIIDL TG+       SN      +K  +ENIVLIW FP  L AR IKECI K FG  
Sbjct: 549 RGDIIDLRTGH-------SNADNWRVSKFKYENIVLIWNFPRKLKARGIKECICKAFGSA 608

Query: 610 NVVSVYHLDESAVFVQFAKPEMVSEFLDLKENLERHDDPISVLHPLTKLFEARTTHAAGY 669
           +V SVYH+D+SAVFV F   E+V +FL LK  LE  D P+SVLHPL+K+ E   T AA Y
Sbjct: 609 SVTSVYHVDDSAVFVLFKNSELVWDFLALKRQLESSDGPVSVLHPLSKILEGGNTGAADY 647

Query: 670 EAYKKICSSPISKLLFADQAEAVGINQKT 698
           EAYK+ICSS +S+++F+DQAE VG+  +T
Sbjct: 669 EAYKEICSSHVSEVMFSDQAETVGVKSRT 647

BLAST of CmUC10G186550 vs. TAIR 10
Match: AT1G55870.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 542.3 bits (1396), Expect = 5.9e-154
Identity = 287/547 (52.47%), Postives = 362/547 (66.18%), Query Frame = 0

Query: 152 MEFLAKYQFDFNACIREGISYLSRAHEDAARKRLNFAYGDEMSDASSNVIRDKKRIGIAD 211
           M+FLAKYQFDFN CI EGISYLSR  E+ A KRL   +G++  D SS    + K + +AD
Sbjct: 1   MDFLAKYQFDFNTCIHEGISYLSRREEEEASKRLKMLHGEDGID-SSGETEELKLVRLAD 60

Query: 212 VLFKERMKNRLHEWRDRLLSERNMEFQGQEGSNDYQRQFQTIFFKMRPALTLSDFTSHQI 271
           VLF  RM+  L+EWR  LL   N   +    SN   +  +T+F  MRPAL+L  FTSHQ+
Sbjct: 61  VLFAARMEKLLNEWRSGLLHGGNASSEFPRISNGSNQSMETVFHHMRPALSLKGFTSHQL 120

Query: 272 RLIRLKLSVSDIKYFSNSTFCYLFIKSYPCLAACEHMQVIKKHFEDLCFVVVNDGDDNLQ 331
           R++                                   V++KHF DL ++  ND   + +
Sbjct: 121 RVLN---------------------------------SVLRKHFGDLVYIHSNDKSSSSR 180

Query: 332 QLVVYVDSRDDKELLMQEVNSDQKEAAEMKIQSAIGFRHVIDLLASEKKLIIGHNCFLAD 391
            +VVY DS  DKE LM+E   ++K  AE KIQSAIGFR VIDLLASEKKLI+GHNCFL  
Sbjct: 181 DIVVYTDSDSDKENLMKEAKDERKRLAERKIQSAIGFRQVIDLLASEKKLIVGHNCFL-- 240

Query: 392 TSVFGPDIAHIHTKFIGSLPSTAEEFVSSIGKHFPYIMDTKILLNGNSILQRRMKKSSTS 451
                 DIAH+++KF+G LPSTAE+FV+SI  HFPYI+DTKILLN N +L +RMKKSSTS
Sbjct: 241 ------DIAHVYSKFVGPLPSTAEKFVASINSHFPYIVDTKILLNVNPMLHQRMKKSSTS 300

Query: 452 LSSAFGLLCPHIAFSSQSNPAF-KSGVRVEVQVDDLRSSNWNSGDKHEAGYDAFMTGCVF 511
           LSSAF  LCP I FSS+S+ +F +  V ++V++D++R SNWN+G KHEAGYDAFMTGC+F
Sbjct: 301 LSSAFSSLCPQIEFSSRSSDSFLQQRVNIDVEIDNVRCSNWNAGGKHEAGYDAFMTGCIF 360

Query: 512 AQACNLIGIDFALRSPSENLVDNENLQKHVNHLYLSWASGDIIDLSTGNRMTESLRSNRS 571
           AQACN +G DF   S  ++   NE L+K++N LYLSW  GDIIDL TG+       SN  
Sbjct: 361 AQACNHLGFDFKQHSQLDDFAQNEKLEKYINRLYLSWTRGDIIDLRTGH-------SNAD 420

Query: 572 KKHQAKISFENIVLIWGFPTHLNAREIKECISKVFGLTNVVSVYHLDESAVFVQFAKPEM 631
               +K  +ENIVLIW FP  L AR IKECI K FG  +V SVYH+D+SAVFV F   E+
Sbjct: 421 NWRVSKFKYENIVLIWNFPRKLKARGIKECICKAFGSASVTSVYHVDDSAVFVLFKNSEL 480

Query: 632 VSEFLDLKENLERHDDPISVLHPLTKLFEARTTHAAGYEAYKKICSSPISKLLFADQAEA 691
           V +FL LK  LE  D P+SVLHPL+K+ E   T AA YEAYK+ICSS +S+++F+DQAE 
Sbjct: 481 VWDFLALKRQLESSDGPVSVLHPLSKILEGGNTGAADYEAYKEICSSHVSEVMFSDQAET 498

Query: 692 VGINQKT 698
           VG+  +T
Sbjct: 541 VGVKSRT 498

BLAST of CmUC10G186550 vs. TAIR 10
Match: AT3G25430.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 200.7 bits (509), Expect = 4.2e-51
Identity = 166/664 (25.00%), Postives = 293/664 (44.13%), Query Frame = 0

Query: 20  AAYSSAAVTAPTIASNSSFPLKNVTKLNFEAALSELRTHVRAADFVAVDLEMTGITSAPW 79
           ++ S+ A    T+ +   + +K V K NF   L E+RT + ++DF+A+ L+ TG  +A W
Sbjct: 7   SSISATAGNTKTL-NQGRWSVKQVKKSNFHVTLDEIRTSIDSSDFIALSLQNTGSYAAAW 66

Query: 80  RESLEFDRFDIRYLKVKDSAEKFAVVQFGVCPFRWDSAKNSFVAHPHNFFVFPRHEIQVD 139
                 D     YLK K +AE++ ++QF +CPF    +K     HP+NF +FPR E++  
Sbjct: 67  HRVSAIDTPQTSYLKAKYAAERYQILQFALCPFSLQGSK--LTVHPYNFHLFPRDELKCG 126

Query: 140 GPSSEFLCQTTSMEFLAKYQFDFNACIREGISYLSRAHEDAARKRLNFAYGDEMSDASSN 199
            PS  F CQ + +  +A+  FDFN CI EGISYLSRA E A++      +  E    + +
Sbjct: 127 MPSYSFSCQASRLTAMAREGFDFNICIYEGISYLSRAQESASK------FLSENPILADS 186

Query: 200 VIRDKKRIGIADVLFKERMKNRLHEWRDRLLSERNMEFQGQEGSNDYQRQFQTIF----- 259
           V        +AD +F  R+++R+  WR   +   +     + G +D     + +      
Sbjct: 187 VTVSSSPATVADTVFVGRIRSRVKNWRQSCIDSGS-----KTGDDDLVSSLRRLVLGSEQ 246

Query: 260 FKMRPALTLSDFTSHQIRLIRLKLSVSDIKYFSNSTFCYLFIKSYPCLAACEHMQVIKKH 319
           +  R  LT+   +  Q++LI                                 ++++ + 
Sbjct: 247 YGSRLCLTIDVCSERQVQLI---------------------------------LEMLTEF 306

Query: 320 FEDLCFVVVNDGDDNLQQL-VVYVDSRDDKELLMQEVNSDQKEAAEMKIQSAIGFRHVID 379
            +D+  ++V       Q +  V++ S++DK+L  +E+   +KE    + +   GFR V+D
Sbjct: 307 SDDVVPLLVASKSRGTQAVRTVFMSSKEDKDLFKRELKDLEKE----ENRRVRGFREVVD 366

Query: 380 LLASEKKLIIGHNCFLADTSVFGPDIAHIHTKFIGSLPSTAEEFVSSIGKHFPYIMDTKI 439
            ++S +K ++  N        +  D   IH KF+G LPS  ++F SS+   FP ++D   
Sbjct: 367 FISSSQKPVVSQN--------YLSDFTSIHAKFLGPLPSNVDDFSSSLSSAFPNVVDLSQ 426

Query: 440 LLNGNSILQRRMKKSSTSLSSAFGLLCP-HIAFSSQSNPAFKSGVRVEVQVDDLRSSNWN 499
            +   S L   +     ++SS      P  +  ++Q  P         V++D+   S+  
Sbjct: 427 FMKEISPLS-NISNLPAAMSSLNRFFAPVDVEVANQGCP---------VKLDEGHQSH-- 486

Query: 500 SGDKHEAGYDAFMTGCVFAQACNLIGIDFALRSPSENLVDNENLQKHVNHLYLSWASGDI 559
                  G +A M   +FA+ C +       +S    +  NE+ Q        S    + 
Sbjct: 487 -------GQNAVMISQLFAKLCTI------QKSDLSTIQSNEDFQ-----ALASDEHANS 546

Query: 560 IDLSTGNRMTESLRSNRSKKHQAKISFENIVLIWGFPTHLNAREIKECISKVFGL-TNVV 619
           +   + N   E+++     K+  ++S EN+V IWG    + A ++K  + K   +     
Sbjct: 547 VTSCSKNAGDENVKV--WSKNSRRVSSENLVFIWGLGKKMTAAKLKNVLQKSHPVFAREF 572

Query: 620 SVYHLDESAVFVQFAKPEMVSEFLDLKENLERHDDPISVLHPLTKLFEARTTHAAGYEAY 676
            V ++D S+  + F +      FL    N E+ D  +       +   A     AGYE Y
Sbjct: 607 DVKYIDRSSAILVFWESGPSETFLSAVNNEEQLDGSL-------REMVAEGLRGAGYETY 572