CmUC10G182530 (gene) Watermelon (USVL531) v1

Overview
NameCmUC10G182530
Typegene
OrganismCitrullus mucosospermus (Watermelon (USVL531) v1)
DescriptionPectinesterase
LocationCmU531Chr10: 157608 .. 160971 (+)
RNA-Seq ExpressionCmUC10G182530
SyntenyCmUC10G182530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTATTTAATTTCCTCCCCACCCTGTCCCTAAGCCAAAACCAAAGCTCAATAATTCCATCTTTAATTTATTAATGGCCAAATTAACCGCAAAACGCCTTCTTCAGTTTTGGTGCTTCTTCATCCTCTTGTTTCCCATGCTTTCGGCTACTTCTCCACCGGATCTTGTGAAGATAGAGTGTTTGACTGTACCCGCCTCCCAATTTTCTAACTCAATCCTCTCCACCATCGATGTTGTACAACAGGTTATCGCCATTTTCTCTCCATTCACTAAATTACTCGGTGATTTACGCCTTTCCACCGCCGTTTCCGACTGTCTCGATTTGCTTGACTCTTCTGCTGACCAGTTGAGCTGGTCCCTCTCCGCCACCCAAAATCCCAAGGGTACTTCCTAAATTTGTATATTACTTGCTGCTTTTAAACCTTGCTTTATTATACTGGTTGGTGCATGGCCTTGGAATGCATTCAATTTGTTATTTTCAACTTACAACGTTTGGGTCTCGCTGCTAAGTTGGCGCGCCTTAGTGGGGTTTACTGCGATGGGACATATGTTGTTGGTTTCTCTAAAATCTTTGTGTACACATATTTATTTAGGGAATTATTTTTTACCTAATCTAAGAATTATCTCCTATTTGATTGACTCAATAAAAATAATAGATGATGCAGATCGATCCCATTAATGAATAATGATAACATTAAATTTAAATAAAATTTAAGATTGGAAATTTAAGTTGAATTCGTATTTGGCATATTTATATTATTTACGATTGTGACCTAGAAGCATTACTATTCTTTCTCTAATAAAAATGTTTTTACTTAGTCTCGAGACAAAGGTAAGACACTTCTAGTGAACCAAGAAGTGTACTAGGAAAATCTGTGTTGATCTCATTTTGTTTTAAGTTGTTGAATTATTACGGGGCAAAGGTTTATCTTTATATAAATACCTATTATGATGTTGAATTATGTTTTTACCTAATTTAGGAATTATGTTTTACTTGATTGGCTTAATAAAATAATAGTTAATGAAATCAATTACACATTTATGAATAACAATAATATTAAATTTAAATAAAATTCAGGAATTGTTCCAAATATAGTAATCATATCTAAAATATTTAGAGATATAACACACAAAATAACTTGTATATATAGCACACAAAATTTAAGTTTTGTGTTTATCTTTAATTGAGATATCAATACTGTATATTACTGGACCTAGTAGTTATTATAAGGTCGCATCAATTTCCAACATTACATTAGAGCCTTGGGCACTACTTGTCTTCTTTTGACATGAAAATGCAATATAAATTAATGACCCTCCTGCCTTGCATTACAGCTAAAGACCACAGCACCGGAGATTTGAGTTCCGATCTGAAGACATGGCTGAGCGCTGCAGTGGTTAATCCAGAAACATGCATGGATGGATTTGAGGGCACCAATAGCATTATCAAAGGTCTAGTATCTGGAAGCATCAACCAGTTAACTTCACAGCTCTACGAGCTTCTTTCCATGGTCAAATCAATCCCCAATCAGCCGGCGGCTGACTTTCCTTCCTGGGTGAAATCTGAGGACCGGAACCTGCTGCAACTAAATGACTTCGCCGCCGATGCCACTGTGGCCGCCGATGGGACTGGGGATTTCACCAGTGTGATGGATGCTGTATTAGCAGCCCCTGACAATAGCATACAACGTTATGTTATATACATAAAGAGGGGTGTGTACTTTGAGAACGTGGAGATCAAGAAGAAGAAATGGAATCTAATGATGATTGGAGATGGAATTGATGCCACCACCATATCGGGTAATCGGAGTTTTATTGACGGTTGGACAACATTCCGTTCAGCGACATTTGCTGTAAGTGGAAGAGGATTCATAGCTCGAGACATAACATTCGAGAATACTGCAGGGCCACAGAAACATCAAGCAGTAGCACTTAGATCGGACTCGGATCTGTCGGTATTTTTTAGATGCCGTATTAGAGGATATCAAGACACTCTGTACACGCACACCATGCGCCAATTCTACCGCGAGTGCCAAATCAGTGGCACCGTTGATTTTCTGTTCGGCGATGCCACCGTCGTTTTCCAAAATTGTAGCATCCTCGCGAAGAAAGGTCTTCCCAATCAGAAAAACACCATCACTGCTCAAGGCCGGAAAGACCCAAACCAACCCACCGGCTTCTCCATTCAATTCTGCAACATCTCTGCGGATTCAGATCTTAAACCTTCGGTTAACACCACAGCTACATACCTTGGAAGACCATGGAAAGAATATTCTAGAACCATTATCATGCAGTCATTTATGAGCGACGCCATTAGGCCAGAAGGTTGGCTTGAGTGGAACGCCGATTTTGCATTGAAGACACTATTCTACGCTGAGTTTATGAATTATGGACCAGGGGCGGGATTGGCGAAGCGTGTCACTTGGCCTGGTTACCGCCGGCTGAACCAAACTAGTGAAGCTTCTAACTTTACCGTGGCTCAATTCATTGAAGGAAACCTGTGGTTGCCGTCCACCGGTGTCAAATACACTGCAGGATTGGGAGCTAATTAAACATGGACCAACACTTGGTGCATCCTTCCTATCTTTACCAATCTCACTTCTCACTTCTTCATATACATAAAAGAATTAACTTATTTTTGGAAAATTATAAAAAAAATTTCATGTCATAATTTATAATTGGCCAATTTGGTGGGATACACAAATATGAGTGTAGGTATGTGTGAAAATGACACAAATGACCTATATTTAAAGGTCTAAATGAGGCGTGACCCGCTTTTAGGGACGAATGGGTCTTGGAGTGAAATTACTAAGTTTTAAGTTTTAAGTTTTAAACTTTTTTTTTTGACATAGTTTAGTTCGTAAGGACCAAAACCGAATAGATATGAAACTCAAACCTTAGCAAATGGAAGCATATTTTGCCAAGGTTTTATTATTAGTATTTAAAAAAAAAAATTGGTCTTTGAATTTTGATGAATGAATGTATTTGATTCTTAATGTTTAAAAGGAAATTATCTTAAATTGCAAAACAGCTGAAAATATTTATAACTAATAGCAAAATATACCATCTATTTGCGATCTATCGCTATCTATCTTGAAATTTTGCTATATTTATAAATATTTTAATTTTTTTTTTTTTGCTATATTTGAAAAGAAACAGGTTTAACCTTAGATATACTATTTTAGTACGGAGTTTTCAAAAATAAATTTAAGTTAAAGGTCTCCCCGTCAACAAAACTATTAATTTTAAATAGAAATTTATCTATGCCATTTTATTTATTTTTTACATTTTCCAATAACATCACCCTAATTTATTTTTAAACTAATTGAATATTATAATATTAAAACTCTCTAAAATATATATT

mRNA sequence

ATTTATTTAATTTCCTCCCCACCCTGTCCCTAAGCCAAAACCAAAGCTCAATAATTCCATCTTTAATTTATTAATGGCCAAATTAACCGCAAAACGCCTTCTTCAGTTTTGGTGCTTCTTCATCCTCTTGTTTCCCATGCTTTCGGCTACTTCTCCACCGGATCTTGTGAAGATAGAGTGTTTGACTGTACCCGCCTCCCAATTTTCTAACTCAATCCTCTCCACCATCGATGTTGTACAACAGGTTATCGCCATTTTCTCTCCATTCACTAAATTACTCGGTGATTTACGCCTTTCCACCGCCGTTTCCGACTGTCTCGATTTGCTTGACTCTTCTGCTGACCAGTTGAGCTGGTCCCTCTCCGCCACCCAAAATCCCAAGGCTAAAGACCACAGCACCGGAGATTTGAGTTCCGATCTGAAGACATGGCTGAGCGCTGCAGTGGTTAATCCAGAAACATGCATGGATGGATTTGAGGGCACCAATAGCATTATCAAAGGTCTAGTATCTGGAAGCATCAACCAGTTAACTTCACAGCTCTACGAGCTTCTTTCCATGGTCAAATCAATCCCCAATCAGCCGGCGGCTGACTTTCCTTCCTGGGTGAAATCTGAGGACCGGAACCTGCTGCAACTAAATGACTTCGCCGCCGATGCCACTGTGGCCGCCGATGGGACTGGGGATTTCACCAGTGTGATGGATGCTGTATTAGCAGCCCCTGACAATAGCATACAACGTTATGTTATATACATAAAGAGGGGTGTGTACTTTGAGAACGTGGAGATCAAGAAGAAGAAATGGAATCTAATGATGATTGGAGATGGAATTGATGCCACCACCATATCGGGTAATCGGAGTTTTATTGACGGTTGGACAACATTCCGTTCAGCGACATTTGCTGTAAGTGGAAGAGGATTCATAGCTCGAGACATAACATTCGAGAATACTGCAGGGCCACAGAAACATCAAGCAGTAGCACTTAGATCGGACTCGGATCTGTCGGTATTTTTTAGATGCCGTATTAGAGGATATCAAGACACTCTGTACACGCACACCATGCGCCAATTCTACCGCGAGTGCCAAATCAGTGGCACCGTTGATTTTCTGTTCGGCGATGCCACCGTCGTTTTCCAAAATTGTAGCATCCTCGCGAAGAAAGGTCTTCCCAATCAGAAAAACACCATCACTGCTCAAGGCCGGAAAGACCCAAACCAACCCACCGGCTTCTCCATTCAATTCTGCAACATCTCTGCGGATTCAGATCTTAAACCTTCGGTTAACACCACAGCTACATACCTTGGAAGACCATGGAAAGAATATTCTAGAACCATTATCATGCAGTCATTTATGAGCGACGCCATTAGGCCAGAAGGTTGGCTTGAGTGGAACGCCGATTTTGCATTGAAGACACTATTCTACGCTGAGTTTATGAATTATGGACCAGGGGCGGGATTGGCGAAGCGTGTCACTTGGCCTGGTTACCGCCGGCTGAACCAAACTAGTGAAGCTTCTAACTTTACCGTGGCTCAATTCATTGAAGGAAACCTGTGGTTGCCGTCCACCGGTGTCAAATACACTGCAGGATTGGGAGCTAATTAAACATGGACCAACACTTGGTGCATCCTTCCTATCTTTACCAATCTCACTTCTCACTTCTTCATATACATAAAAGAATTAACTTATTTTTGGAAAATTATAAAAAAAATTTCATGTCATAATTTATAATTGGCCAATTTGGTGGGATACACAAATATGAGTGTAGGTATGTGTGAAAATGACACAAATGACCTATATTTAAAGGTCTAAATGAGGCGTGACCCGCTTTTAGGGACGAATGGGTCTTGGAGTGAAATTACTAAGTTTTAAGTTTTAAGTTTTAAACTTTTTTTTTTGACATAGTTTAGTTCGTAAGGACCAAAACCGAATAGATATGAAACTCAAACCTTAGCAAATGGAAGCATATTTTGCCAAGGTTTTATTATTAGTATTTAAAAAAAAAAATTGGTCTTTGAATTTTGATGAATGAATGTATTTGATTCTTAATGTTTAAAAGGAAATTATCTTAAATTGCAAAACAGCTGAAAATATTTATAACTAATAGCAAAATATACCATCTATTTGCGATCTATCGCTATCTATCTTGAAATTTTGCTATATTTATAAATATTTTAATTTTTTTTTTTTTGCTATATTTGAAAAGAAACAGGTTTAACCTTAGATATACTATTTTAGTACGGAGTTTTCAAAAATAAATTTAAGTTAAAGGTCTCCCCGTCAACAAAACTATTAATTTTAAATAGAAATTTATCTATGCCATTTTATTTATTTTTTACATTTTCCAATAACATCACCCTAATTTATTTTTAAACTAATTGAATATTATAATATTAAAACTCTCTAAAATATATATT

Coding sequence (CDS)

ATGGCCAAATTAACCGCAAAACGCCTTCTTCAGTTTTGGTGCTTCTTCATCCTCTTGTTTCCCATGCTTTCGGCTACTTCTCCACCGGATCTTGTGAAGATAGAGTGTTTGACTGTACCCGCCTCCCAATTTTCTAACTCAATCCTCTCCACCATCGATGTTGTACAACAGGTTATCGCCATTTTCTCTCCATTCACTAAATTACTCGGTGATTTACGCCTTTCCACCGCCGTTTCCGACTGTCTCGATTTGCTTGACTCTTCTGCTGACCAGTTGAGCTGGTCCCTCTCCGCCACCCAAAATCCCAAGGCTAAAGACCACAGCACCGGAGATTTGAGTTCCGATCTGAAGACATGGCTGAGCGCTGCAGTGGTTAATCCAGAAACATGCATGGATGGATTTGAGGGCACCAATAGCATTATCAAAGGTCTAGTATCTGGAAGCATCAACCAGTTAACTTCACAGCTCTACGAGCTTCTTTCCATGGTCAAATCAATCCCCAATCAGCCGGCGGCTGACTTTCCTTCCTGGGTGAAATCTGAGGACCGGAACCTGCTGCAACTAAATGACTTCGCCGCCGATGCCACTGTGGCCGCCGATGGGACTGGGGATTTCACCAGTGTGATGGATGCTGTATTAGCAGCCCCTGACAATAGCATACAACGTTATGTTATATACATAAAGAGGGGTGTGTACTTTGAGAACGTGGAGATCAAGAAGAAGAAATGGAATCTAATGATGATTGGAGATGGAATTGATGCCACCACCATATCGGGTAATCGGAGTTTTATTGACGGTTGGACAACATTCCGTTCAGCGACATTTGCTGTAAGTGGAAGAGGATTCATAGCTCGAGACATAACATTCGAGAATACTGCAGGGCCACAGAAACATCAAGCAGTAGCACTTAGATCGGACTCGGATCTGTCGGTATTTTTTAGATGCCGTATTAGAGGATATCAAGACACTCTGTACACGCACACCATGCGCCAATTCTACCGCGAGTGCCAAATCAGTGGCACCGTTGATTTTCTGTTCGGCGATGCCACCGTCGTTTTCCAAAATTGTAGCATCCTCGCGAAGAAAGGTCTTCCCAATCAGAAAAACACCATCACTGCTCAAGGCCGGAAAGACCCAAACCAACCCACCGGCTTCTCCATTCAATTCTGCAACATCTCTGCGGATTCAGATCTTAAACCTTCGGTTAACACCACAGCTACATACCTTGGAAGACCATGGAAAGAATATTCTAGAACCATTATCATGCAGTCATTTATGAGCGACGCCATTAGGCCAGAAGGTTGGCTTGAGTGGAACGCCGATTTTGCATTGAAGACACTATTCTACGCTGAGTTTATGAATTATGGACCAGGGGCGGGATTGGCGAAGCGTGTCACTTGGCCTGGTTACCGCCGGCTGAACCAAACTAGTGAAGCTTCTAACTTTACCGTGGCTCAATTCATTGAAGGAAACCTGTGGTTGCCGTCCACCGGTGTCAAATACACTGCAGGATTGGGAGCTAATTAA

Protein sequence

MAKLTAKRLLQFWCFFILLFPMLSATSPPDLVKIECLTVPASQFSNSILSTIDVVQQVIAIFSPFTKLLGDLRLSTAVSDCLDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGSINQLTSQLYELLSMVKSIPNQPAADFPSWVKSEDRNLLQLNDFAADATVAADGTGDFTSVMDAVLAAPDNSIQRYVIYIKRGVYFENVEIKKKKWNLMMIGDGIDATTISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSFMSDAIRPEGWLEWNADFALKTLFYAEFMNYGPGAGLAKRVTWPGYRRLNQTSEASNFTVAQFIEGNLWLPSTGVKYTAGLGAN
Homology
BLAST of CmUC10G182530 vs. NCBI nr
Match: XP_004146188.2 (pectinesterase/pectinesterase inhibitor PPE8B [Cucumis sativus] >XP_031738323.1 pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis sativus] >KGN55582.1 hypothetical protein Csa_011581 [Cucumis sativus])

HSP 1 Score: 933.3 bits (2411), Expect = 8.7e-268
Identity = 464/509 (91.16%), Postives = 490/509 (96.27%), Query Frame = 0

Query: 1   MAKLTAKRLLQFWCFFILLFPM-LSATSPPDLVKIECLTVPASQFSNSILSTIDVVQQVI 60
           MAKLT  + LQF C  ILLFPM L A+SPPDLV+ ECL+VP+SQFSNS+LSTIDVV+QV+
Sbjct: 70  MAKLTT-QTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVM 129

Query: 61  AIFSPFTKLLGDLRLSTAVSDCLDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTW 120
           AIFSPF+KLLGD RLSTA+SDCLDLLDSSADQLSWSLSATQNPKAK+HSTGDLSSDLKTW
Sbjct: 130 AIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTW 189

Query: 121 LSAAVVNPETCMDGFEGTNSIIKGLVSGSINQLTSQLYELLSMVKSIPNQPAADFPSWVK 180
           LSAAVVNPETCMDGFEGTNSIIKGLVSG +NQLTSQLY+LLSMVKSIPNQP ++FPSW+K
Sbjct: 190 LSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQP-SEFPSWLK 249

Query: 181 SEDRNLLQLNDFAADATVAADGTGDFTSVMDAVLAAPDNSIQRYVIYIKRGVYFENVEIK 240
           SED+NLLQ+ND AADATVAADGTGDFT+VMDAVLAAPDNSI+RYVIYIK+GVY ENVEIK
Sbjct: 250 SEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIK 309

Query: 241 KKKWNLMMIGDGIDATTISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQ 300
           KKKWNLMMIGDGIDAT ISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAG +KHQ
Sbjct: 310 KKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQ 369

Query: 301 AVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSIL 360
           AVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSIL
Sbjct: 370 AVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSIL 429

Query: 361 AKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRT 420
           AKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRT
Sbjct: 430 AKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRT 489

Query: 421 IIMQSFMSDAIRPEGWLEWNADFALKTLFYAEFMNYGPGAGLAKRVTWPGYRRLNQTSEA 480
           IIMQS++SDAIRPEGWLEWNA+FAL TLFYAEFMNYGPGAGLAKRV WPGY RLNQTSEA
Sbjct: 490 IIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEA 549

Query: 481 SNFTVAQFIEGNLWLPSTGVKYTAGLGAN 509
           +NFTVAQFIEGNLWLPSTGVKYTAGLGAN
Sbjct: 550 TNFTVAQFIEGNLWLPSTGVKYTAGLGAN 576

BLAST of CmUC10G182530 vs. NCBI nr
Match: XP_008448427.1 (PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis melo])

HSP 1 Score: 931.8 bits (2407), Expect = 2.5e-267
Identity = 464/509 (91.16%), Postives = 488/509 (95.87%), Query Frame = 0

Query: 1   MAKLTAKRLLQFWCFFILLFPM-LSATSPPDLVKIECLTVPASQFSNSILSTIDVVQQVI 60
           MAKLT  ++LQF C FILLFPM L A+SP DLV+ ECL+VP+SQFSNS+LSTIDVV+QV+
Sbjct: 70  MAKLTT-QILQFCCIFILLFPMLLPASSPADLVQTECLSVPSSQFSNSLLSTIDVVRQVM 129

Query: 61  AIFSPFTKLLGDLRLSTAVSDCLDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTW 120
           AIFSPF+KLLGD RLSTA+SDCLDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTW
Sbjct: 130 AIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTW 189

Query: 121 LSAAVVNPETCMDGFEGTNSIIKGLVSGSINQLTSQLYELLSMVKSIPNQPAADFPSWVK 180
           LSAAVVNPETCMDGFEGTNSIIKGLVSG INQLTSQLY+LLSMVKSIPNQP ++FPSW+K
Sbjct: 190 LSAAVVNPETCMDGFEGTNSIIKGLVSGGINQLTSQLYDLLSMVKSIPNQP-SEFPSWLK 249

Query: 181 SEDRNLLQLNDFAADATVAADGTGDFTSVMDAVLAAPDNSIQRYVIYIKRGVYFENVEIK 240
           SEDRNLLQLND AADATVAADGTGDFT+VMDAVLAAPD S +RYVIYIK+GVY ENVEIK
Sbjct: 250 SEDRNLLQLNDLAADATVAADGTGDFTNVMDAVLAAPDKSTRRYVIYIKKGVYLENVEIK 309

Query: 241 KKKWNLMMIGDGIDATTISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQ 300
           KKKWNLMMIGDGIDAT ISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAG +KHQ
Sbjct: 310 KKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQ 369

Query: 301 AVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSIL 360
           AVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSIL
Sbjct: 370 AVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSIL 429

Query: 361 AKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRT 420
           AKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRT
Sbjct: 430 AKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRT 489

Query: 421 IIMQSFMSDAIRPEGWLEWNADFALKTLFYAEFMNYGPGAGLAKRVTWPGYRRLNQTSEA 480
           IIMQS++SDAIRPEGWLEWN +FAL TLFYAEFMNYGPGAGLAKRV WPGY RLNQTSEA
Sbjct: 490 IIMQSYISDAIRPEGWLEWNGNFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEA 549

Query: 481 SNFTVAQFIEGNLWLPSTGVKYTAGLGAN 509
           +NFTVAQFIEGN+WLPSTGVKYTAGLGAN
Sbjct: 550 TNFTVAQFIEGNMWLPSTGVKYTAGLGAN 576

BLAST of CmUC10G182530 vs. NCBI nr
Match: XP_038905308.1 (pectinesterase/pectinesterase inhibitor PPE8B-like [Benincasa hispida])

HSP 1 Score: 912.9 bits (2358), Expect = 1.2e-261
Identity = 455/509 (89.39%), Postives = 476/509 (93.52%), Query Frame = 0

Query: 1   MAKLTAKRLLQFWC-FFILLFPMLSATSPPDLVKIECLTVPASQFSNSILSTIDVVQQVI 60
           MAKLT K  LQF C FFILLFP+L ATS  DLV+ ECL VP SQFSNS+LSTID V+QV+
Sbjct: 1   MAKLTTKP-LQFCCFFFILLFPILPATSSVDLVQTECLKVPFSQFSNSLLSTIDGVRQVM 60

Query: 61  AIFSPFTKLLGDLRLSTAVSDCLDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTW 120
            IFSPFTKLLGDLRLSTA+SDCLDLLDSS DQLSWSLSATQNPKAKD+STGDLSSDLKTW
Sbjct: 61  TIFSPFTKLLGDLRLSTAISDCLDLLDSSVDQLSWSLSATQNPKAKDYSTGDLSSDLKTW 120

Query: 121 LSAAVVNPETCMDGFEGTNSIIKGLVSGSINQLTSQLYELLSMVKSIPNQPAADFPSWVK 180
           LSAAVVNPETC+DGFEGTNS IKGLVSG I+QLTSQLY+LLSMVKS PN   A+FPSWVK
Sbjct: 121 LSAAVVNPETCIDGFEGTNSFIKGLVSGGIDQLTSQLYDLLSMVKSFPNNQPAEFPSWVK 180

Query: 181 SEDRNLLQLNDFAADATVAADGTGDFTSVMDAVLAAPDNSIQRYVIYIKRGVYFENVEIK 240
           SEDRNLL LND AADATVAADGTGDFTSVMDAV AAPDNSIQRYVIYIKRGVY ENVEIK
Sbjct: 181 SEDRNLLHLNDLAADATVAADGTGDFTSVMDAVRAAPDNSIQRYVIYIKRGVYLENVEIK 240

Query: 241 KKKWNLMMIGDGIDATTISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQ 300
           KKKWNLMMIGDGIDAT ISG R+F+DGWTTFRSATFAVSGRGFIARDITFENTAGP+KHQ
Sbjct: 241 KKKWNLMMIGDGIDATIISGKRNFVDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQ 300

Query: 301 AVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSIL 360
           AVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGD TVVFQNC+IL
Sbjct: 301 AVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDGTVVFQNCTIL 360

Query: 361 AKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRT 420
           AKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDL+P VNTTATYLGRPWKEYSRT
Sbjct: 361 AKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLEPLVNTTATYLGRPWKEYSRT 420

Query: 421 IIMQSFMSDAIRPEGWLEWNADFALKTLFYAEFMNYGPGAGLAKRVTWPGYRRLNQTSEA 480
           IIMQS+MSDAIRPEGWLEWN DFAL TLFYAEFMN+GPGA LAKRV WPGYRRLN TSEA
Sbjct: 421 IIMQSYMSDAIRPEGWLEWNGDFALNTLFYAEFMNHGPGARLAKRVNWPGYRRLNHTSEA 480

Query: 481 SNFTVAQFIEGNLWLPSTGVKYTAGLGAN 509
           +NFTVAQFIEGN+WLPSTGVK+TAGLGAN
Sbjct: 481 TNFTVAQFIEGNMWLPSTGVKFTAGLGAN 508

BLAST of CmUC10G182530 vs. NCBI nr
Match: KAA0052899.1 (pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis melo var. makuwa] >TYK11353.1 pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis melo var. makuwa])

HSP 1 Score: 911.8 bits (2355), Expect = 2.7e-261
Identity = 448/487 (91.99%), Postives = 471/487 (96.71%), Query Frame = 0

Query: 22  MLSATSPPDLVKIECLTVPASQFSNSILSTIDVVQQVIAIFSPFTKLLGDLRLSTAVSDC 81
           +L A+SP DLV+ ECL+VP+SQFSNS+LSTIDVV+QV+AIFSPF+KLLGD RLSTA+SDC
Sbjct: 2   LLPASSPADLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDC 61

Query: 82  LDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSII 141
           LDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSII
Sbjct: 62  LDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSII 121

Query: 142 KGLVSGSINQLTSQLYELLSMVKSIPNQPAADFPSWVKSEDRNLLQLNDFAADATVAADG 201
           KGLVSG INQLTSQLY+LLSMVKSIPNQP ++FPSW+KSEDRNLLQLND AADATVAADG
Sbjct: 122 KGLVSGGINQLTSQLYDLLSMVKSIPNQP-SEFPSWLKSEDRNLLQLNDLAADATVAADG 181

Query: 202 TGDFTSVMDAVLAAPDNSIQRYVIYIKRGVYFENVEIKKKKWNLMMIGDGIDATTISGNR 261
           TGDFT+VMDAVLAAPD S +RYVIYIK+GVY ENVEIKKKKWNLMMIGDGIDAT ISGNR
Sbjct: 182 TGDFTNVMDAVLAAPDKSTRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNR 241

Query: 262 SFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVALRSDSDLSVFFRCRIRGYQ 321
           SFIDGWTTFRSATFAVSGRGFIARDITFENTAG +KHQAVALRSDSDLSVFFRCRIRGYQ
Sbjct: 242 SFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQ 301

Query: 322 DTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQ 381
           DTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQ
Sbjct: 302 DTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQ 361

Query: 382 PTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSFMSDAIRPEGWLEWNAD 441
           PTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQS++SDAIRPEGWLEWN +
Sbjct: 362 PTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNGN 421

Query: 442 FALKTLFYAEFMNYGPGAGLAKRVTWPGYRRLNQTSEASNFTVAQFIEGNLWLPSTGVKY 501
           FAL TLFYAEFMNYGPGAGLAKRV WPGY RLNQTSEA+NFTVAQFIEGN+WLPSTGVKY
Sbjct: 422 FALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNMWLPSTGVKY 481

Query: 502 TAGLGAN 509
           TAGLGAN
Sbjct: 482 TAGLGAN 487

BLAST of CmUC10G182530 vs. NCBI nr
Match: XP_022949597.1 (pectinesterase/pectinesterase inhibitor PPE8B-like [Cucurbita moschata])

HSP 1 Score: 871.7 bits (2251), Expect = 3.1e-249
Identity = 432/513 (84.21%), Postives = 473/513 (92.20%), Query Frame = 0

Query: 1   MAKLTAKRLLQFWCFFIL-LFPMLSATSPPDLVKIECLTVPASQFSNSILSTIDVVQQVI 60
           MAKLT K  L+FW F +L LFPM  A    DL + ECL VP+S+FSNS+LSTI VV+QV+
Sbjct: 1   MAKLT-KTHLKFWLFMLLILFPMAPAA---DLEQTECLKVPSSEFSNSVLSTIGVVRQVM 60

Query: 61  AIFSPFTKLLGDLRLSTAVSDCLDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTW 120
            IFSPFTK LGD RLSTA+SDCLDLLDSS+DQLSWSLSA QNPKAK++STGDLSSDLKTW
Sbjct: 61  LIFSPFTKFLGDSRLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTW 120

Query: 121 LSAAVVNPETCMDGFEGTNSIIKGLVSGSINQLTSQLYELLSMVKSIPNQPA----ADFP 180
           LSAA VNPETCM+GFEGTNSI+KGLVS  INQLTSQ+Y LLSMVKSI NQP     ++FP
Sbjct: 121 LSAAGVNPETCMEGFEGTNSIVKGLVSEGINQLTSQVYNLLSMVKSISNQPVGKGESEFP 180

Query: 181 SWVKSEDRNLLQLNDFAADATVAADGTGDFTSVMDAVLAAPDNSIQRYVIYIKRGVYFEN 240
           +W+K+E+++LLQ NDFAADATVAADGTGDFT+VM+AVLAAPD S++RYVIY+K+GVYFEN
Sbjct: 181 AWLKTEEQSLLQQNDFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFEN 240

Query: 241 VEIKKKKWNLMMIGDGIDATTISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGP 300
           VEIKKKKWN+MMIGDGIDAT ISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGP
Sbjct: 241 VEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGP 300

Query: 301 QKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQN 360
           +KHQAVALRSDSDLSVFFRCR+RGYQDTLYTHTMRQFYREC ISGTVDFLFGDATVVFQN
Sbjct: 301 EKHQAVALRSDSDLSVFFRCRMRGYQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQN 360

Query: 361 CSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKE 420
           C+ILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDL+PSVN TATYLGRPWKE
Sbjct: 361 CTILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLQPSVNATATYLGRPWKE 420

Query: 421 YSRTIIMQSFMSDAIRPEGWLEWNADFALKTLFYAEFMNYGPGAGLAKRVTWPGYRRLNQ 480
           YSRTIIMQS++S+AIRPEGWLEWN DFAL TLFYAEFMNYGPGAGLAKRV WPGY RLNQ
Sbjct: 421 YSRTIIMQSYVSNAIRPEGWLEWNGDFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQ 480

Query: 481 TSEASNFTVAQFIEGNLWLPSTGVKYTAGLGAN 509
           TSEA+NFTV+QFIEGNLWLPSTGVK+T+G GAN
Sbjct: 481 TSEATNFTVSQFIEGNLWLPSTGVKFTSGFGAN 509

BLAST of CmUC10G182530 vs. ExPASy Swiss-Prot
Match: Q43062 (Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 730.7 bits (1885), Expect = 1.1e-209
Identity = 358/501 (71.46%), Postives = 418/501 (83.43%), Query Frame = 0

Query: 9   LLQFWCFFILLFPMLSATSPPDLVKIECLTVPASQFSNSILSTIDVVQQVIAIFSPFTKL 68
           L+    FF   F   ++ S  D ++ +CL V A++F+ S+  TID VQQV +I S F   
Sbjct: 21  LVLLCAFFSSSFIPFASCSITDDLQTQCLKVSATEFAGSLKDTIDAVQQVASILSQFANA 80

Query: 69  LGDLRLSTAVSDCLDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTWLSAAVVNPE 128
            GD RL+ A+SDCLDLLD SAD+L+WSLSA+QN K K++STG LSSDL+TWLSAA+VN +
Sbjct: 81  FGDFRLANAISDCLDLLDFSADELNWSLSASQNQKGKNNSTGKLSSDLRTWLSAALVNQD 140

Query: 129 TCMDGFEGTNSIIKGLVSGSINQLTSQLYELLSMVKSIPNQ--PAADFPSWVKSEDRNLL 188
           TC +GFEGTNSI++GL+S  + Q+TS + ELL+ V    NQ  P    PSWVK++DR LL
Sbjct: 141 TCSNGFEGTNSIVQGLISAGLGQVTSLVQELLTQVHPNSNQQGPNGQIPSWVKTKDRKLL 200

Query: 189 QLNDFAADATVAADGTGDFTSVMDAVLAAPDNSIQRYVIYIKRGVYFENVEIKKKKWNLM 248
           Q +  + DA VA DGTG+FT+V DAVLAAPD S++RYVIYIKRG Y ENVEIKKKKWNLM
Sbjct: 201 QADGVSVDAIVAQDGTGNFTNVTDAVLAAPDYSMRRYVIYIKRGTYKENVEIKKKKWNLM 260

Query: 249 MIGDGIDATTISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVALRSD 308
           MIGDG+DAT ISGNRSF+DGWTTFRSATFAVSGRGFIARDITFENTAGP+KHQAVALRSD
Sbjct: 261 MIGDGMDATIISGNRSFVDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSD 320

Query: 309 SDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPN 368
           SDLSVF+RC IRGYQDTLYTHTMRQFYR+C+ISGTVDF+FGDATVVFQNC ILAKKGLPN
Sbjct: 321 SDLSVFYRCNIRGYQDTLYTHTMRQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLPN 380

Query: 369 QKNTITAQGRKDPNQPTGFSIQFCNISADSDLK-PSVNTTATYLGRPWKEYSRTIIMQSF 428
           QKN+ITAQGRKDPN+PTG SIQFCNI+ADSDL+  SVN+T TYLGRPWK YSRT+IMQSF
Sbjct: 381 QKNSITAQGRKDPNEPTGISIQFCNITADSDLEAASVNSTPTYLGRPWKLYSRTVIMQSF 440

Query: 429 MSDAIRPEGWLEWNADFALKTLFYAEFMNYGPGAGLAKRVTWPGYRRLNQTSEASNFTVA 488
           +S+ IRPEGWLEWN DFAL +LFY E+MNYGPGAGL  RV WPGY+  N++++A N+TVA
Sbjct: 441 LSNVIRPEGWLEWNGDFALNSLFYGEYMNYGPGAGLGSRVKWPGYQVFNESTQAKNYTVA 500

Query: 489 QFIEGNLWLPSTGVKYTAGLG 507
           QFIEGNLWLPSTGVKYTA  G
Sbjct: 501 QFIEGNLWLPSTGVKYTAEFG 521

BLAST of CmUC10G182530 vs. ExPASy Swiss-Prot
Match: Q9LXK7 (Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3702 GN=PME32 PE=2 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 6.2e-192
Identity = 327/525 (62.29%), Postives = 412/525 (78.48%), Query Frame = 0

Query: 1   MAKLTAKRLLQFWCFFILL---FPMLSATSPPDLVKIECLTVPASQFSNSILSTIDVVQQ 60
           MAK        F+ F I++        A S  DLV++ECL VP  +F+ +  + +D + +
Sbjct: 1   MAKFRQMGSSIFFLFLIIISLCSAHKEAFSSTDLVQMECLRVPPLEFAEAAKTVVDAITK 60

Query: 61  VIAIFSPFTKLLGDLRLSTAVSDCLDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLK 120
            +AI S F K  G  R+S A+ DC+DLLDS+A++LSW +SA+Q+P  KD+STGD+ SDL+
Sbjct: 61  AVAIVSKFDKKAGKSRVSNAIVDCVDLLDSAAEELSWIISASQSPNGKDNSTGDVGSDLR 120

Query: 121 TWLSAAVVNPETCMDGFEGTNSIIKGLVSGSINQLTSQLYELLSMVKSIPNQPA------ 180
           TW+SAA+ N +TC+DGFEGTN IIK +V+G ++++ + +  LL+MV S P++P       
Sbjct: 121 TWISAALSNQDTCLDGFEGTNGIIKKIVAGGLSKVGTTVRNLLTMVHSPPSKPKPKPIKA 180

Query: 181 ----------ADFPSWVKSEDRNLLQLNDF-AADATVAADGTGDFTSVMDAVLAAPDNSI 240
                     + FPSWVK  DR LLQ ++   ADA VAADGTG+FT++ DAVLAAPD S 
Sbjct: 181 QTMTKAHSGFSKFPSWVKPGDRKLLQTDNITVADAVVAADGTGNFTTISDAVLAAPDYST 240

Query: 241 QRYVIYIKRGVYFENVEIKKKKWNLMMIGDGIDATTISGNRSFIDGWTTFRSATFAVSGR 300
           +RYVI++KRGVY ENVEIKKKKWN+MM+GDGIDAT I+GNRSFIDGWTTFRSATFAVSGR
Sbjct: 241 KRYVIHVKRGVYVENVEIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGR 300

Query: 301 GFIARDITFENTAGPQKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISG 360
           GFIARDITF+NTAGP+KHQAVA+RSD+DL VF+RC +RGYQDTLY H+MRQF+REC I+G
Sbjct: 301 GFIARDITFQNTAGPEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITG 360

Query: 361 TVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKP 420
           TVDF+FGDAT VFQ+C I AK+GLPNQKN+ITAQGRKDPN+PTGF+IQF NI+AD+DL  
Sbjct: 361 TVDFIFGDATAVFQSCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLL 420

Query: 421 SVNTTATYLGRPWKEYSRTIIMQSFMSDAIRPEGWLEWNADFALKTLFYAEFMNYGPGAG 480
           ++NTTATYLGRPWK YSRT+ MQ++MSDAI P GWLEWN +FAL TL+Y E+MN GPGA 
Sbjct: 421 NLNTTATYLGRPWKLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGAS 480

Query: 481 LAKRVTWPGYRRLNQTSEASNFTVAQFIEGNLWLPSTGVKYTAGL 506
           L +RV WPGY  LN ++EA+NFTV+Q I+GNLWLPSTG+ + AGL
Sbjct: 481 LDRRVKWPGYHVLNTSAEANNFTVSQLIQGNLWLPSTGITFIAGL 525

BLAST of CmUC10G182530 vs. ExPASy Swiss-Prot
Match: Q9SMY7 (Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3702 GN=PME44 PE=2 SV=2)

HSP 1 Score: 671.8 bits (1732), Expect = 6.2e-192
Identity = 328/518 (63.32%), Postives = 400/518 (77.22%), Query Frame = 0

Query: 15  FFILLFPMLSATSPPDLVKIECLTVPASQFSNSILSTIDVVQQVIAIFSPFTKLLGDLRL 74
           +F++L  +    S  +  + + L VPAS+F +SI + + V++QV +I S F    GD RL
Sbjct: 6   YFLILLMLGLCVSSEENFQYDYLKVPASEFVSSINTIVVVIRQVSSILSQFADFSGDRRL 65

Query: 75  STAVSDCLDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTWLSAAVVNPETCMDGF 134
             AVSDCLDLLD S+++L+WS SA++NPK K + TGD+ SD +TWLSAA+ N  TCM+GF
Sbjct: 66  QNAVSDCLDLLDFSSEELTWSASASENPKGKGNGTGDVGSDTRTWLSAALSNQATCMEGF 125

Query: 135 EGTNSIIKGLVSGSINQLTSQLYELLSMV-------------------KSIPNQPAAD-- 194
           +GT+ ++K LV+GS++QL S L ELL +V                   K+ P +   D  
Sbjct: 126 DGTSGLVKSLVAGSLDQLYSMLRELLPLVQPEQKPKAVSKPGPIAKGPKAPPGRKLRDTD 185

Query: 195 ------FPSWVKSEDRNLLQLNDFAADATVAADGTGDFTSVMDAVLAAPDNSIQRYVIYI 254
                 FP WV+ +DR LL+ N    D +VA DGTG+FT +MDA+  APD S  R+VIYI
Sbjct: 186 EDESLQFPDWVRPDDRKLLESNGRTYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYI 245

Query: 255 KRGVYFENVEIKKKKWNLMMIGDGIDATTISGNRSFIDGWTTFRSATFAVSGRGFIARDI 314
           K+G+Y ENVEIKKKKWN++M+GDGID T ISGNRSFIDGWTTFRSATFAVSGRGF+ARDI
Sbjct: 246 KKGLYLENVEIKKKKWNIVMLGDGIDVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDI 305

Query: 315 TFENTAGPQKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFG 374
           TF+NTAGP+KHQAVALRSDSDLSVFFRC +RGYQDTLYTHTMRQFYREC I+GTVDF+FG
Sbjct: 306 TFQNTAGPEKHQAVALRSDSDLSVFFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFG 365

Query: 375 DATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTAT 434
           D TVVFQNC ILAK+GLPNQKNTITAQGRKD NQP+GFSIQF NISAD+DL P +NTT T
Sbjct: 366 DGTVVFQNCQILAKRGLPNQKNTITAQGRKDVNQPSGFSIQFSNISADADLVPYLNTTRT 425

Query: 435 YLGRPWKEYSRTIIMQSFMSDAIRPEGWLEWNADFALKTLFYAEFMNYGPGAGLAKRVTW 494
           YLGRPWK YSRT+ +++ MSD +RPEGWLEWNADFAL TLFY EFMNYGPG+GL+ RV W
Sbjct: 426 YLGRPWKLYSRTVFIRNNMSDVVRPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKW 485

Query: 495 PGYRRLNQTSEASNFTVAQFIEGNLWLPSTGVKYTAGL 506
           PGY   N + +A+NFTV+QFI+GNLWLPSTGV ++ GL
Sbjct: 486 PGYHVFNNSDQANNFTVSQFIKGNLWLPSTGVTFSDGL 523

BLAST of CmUC10G182530 vs. ExPASy Swiss-Prot
Match: Q96575 (Pectinesterase 2.2 OS=Solanum lycopersicum OX=4081 GN=PME2.2 PE=3 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 2.4e-127
Identity = 236/489 (48.26%), Postives = 320/489 (65.44%), Query Frame = 0

Query: 30  DLVKIECLTVPASQFSNSILSTIDVVQQV---IAIFSPFTKLLGDLRLSTAVSDCLDLLD 89
           DL+  E +T  +   S  +   ++ V Q+   I + S     + D+R   A++DCL+LLD
Sbjct: 69  DLMSNEIVTTDSDGLSILMKFLVNYVHQMNNAIPVVSKMKNQINDIRQEGALTDCLELLD 128

Query: 90  SSADQLSWSLSATQNPKAKDHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVS 149
            S D +S S++A       +H      ++ ++WLS  + N  TC+D  +   S  K +++
Sbjct: 129 QSVDLVSDSIAAIDKRTHSEH------ANAQSWLSGVLTNHVTCLDELD---SFTKAMIN 188

Query: 150 G-SINQLTSQLYELLSMVKSI--PNQPA-----ADFPSWVKSEDRNLLQLN--DFAADAT 209
           G ++++L S+    L+M+ S+  PN           PSWV S DR L++ +  D  A+A 
Sbjct: 189 GTNLDELISRAKVALAMLASVTTPNDDVLRPGLGKMPSWVSSRDRKLMESSGKDIGANAV 248

Query: 210 VAADGTGDFTSVMDAVLAAPDNSIQRYVIYIKRGVYFENVEIKKKKWNLMMIGDGIDATT 269
           VA DGTG + ++ +AV AAPD S  RYVIY+KRG+Y ENVE+  +K  LM++GDG+ AT 
Sbjct: 249 VAKDGTGKYRTLAEAVAAAPDKSKTRYVIYVKRGIYKENVEVSSRKMKLMIVGDGMHATI 308

Query: 270 ISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVALRSDSDLSVFFRCR 329
           I+GN + +DG TTF SAT A  G+GFI +DI  +NTAGP KHQAVALR  +D SV  RCR
Sbjct: 309 ITGNLNVVDGSTTFHSATLAAVGKGFILQDICIQNTAGPAKHQAVALRVGADKSVINRCR 368

Query: 330 IRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGR 389
           I  YQDTLY H+ RQFYR+  ++GT+DF+FG+A VVFQ C ++A+K    Q+N +TAQGR
Sbjct: 369 IDAYQDTLYAHSQRQFYRDSYVTGTIDFIFGNAAVVFQKCKLVARKPGKYQQNMVTAQGR 428

Query: 390 KDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSFMSDAIRPEGWL 449
            DPNQ TG SIQFCNI A SDL+P +    TYLGRPWK+YSRT++M+S++   I P GW 
Sbjct: 429 TDPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKKYSRTVVMESYLGGLINPAGWA 488

Query: 450 EWNADFALKTLFYAEFMNYGPGAGLAKRVTWPGYRRLNQTSEASNFTVAQFIEGNLWLPS 506
           EW+ DFALKTL+Y EFMN GPGAG +KRV WPGY  +   +EA  FTVA+ I+G  WL S
Sbjct: 489 EWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHCITDPAEAMPFTVAKLIQGGSWLRS 548

BLAST of CmUC10G182530 vs. ExPASy Swiss-Prot
Match: P14280 (Pectinesterase 1 OS=Solanum lycopersicum OX=4081 GN=PME1.9 PE=1 SV=5)

HSP 1 Score: 456.4 bits (1173), Expect = 4.1e-127
Identity = 238/489 (48.67%), Postives = 319/489 (65.24%), Query Frame = 0

Query: 30  DLVKIECLTVPASQFSNSILSTIDVVQQV---IAIFSPFTKLLGDLRLSTAVSDCLDLLD 89
           DL+  E +T  +   S  +   ++ V Q+   I +       + D+R   A++DCL+LLD
Sbjct: 65  DLISNEIVTTESDGHSILMKFLVNYVHQMNNAIPVVRKMKNQINDIRQHGALTDCLELLD 124

Query: 90  SSADQLSWSLSATQNPKAKDHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVS 149
            S D  S S++A       +H      ++ ++WLS  + N  TC+D  +   S  K +++
Sbjct: 125 QSVDFASDSIAAIDKRSRSEH------ANAQSWLSGVLTNHVTCLDELD---SFTKAMIN 184

Query: 150 G-SINQLTSQLYELLSMVKSIPNQPAADF-------PSWVKSEDRNLLQLN--DFAADAT 209
           G ++ +L S+    L+M+ S+  Q    F       PSWV S DR L++ +  D  A+A 
Sbjct: 185 GTNLEELISRAKVALAMLASLTTQDEDVFMTVLGKMPSWVSSMDRKLMESSGKDIIANAV 244

Query: 210 VAADGTGDFTSVMDAVLAAPDNSIQRYVIYIKRGVYFENVEIKKKKWNLMMIGDGIDATT 269
           VA DGTGD+ ++ +AV AAPD S  RYVIY+KRG Y ENVE+   K NLM++GDG+ ATT
Sbjct: 245 VAQDGTGDYQTLAEAVAAAPDKSKTRYVIYVKRGTYKENVEVASNKMNLMIVGDGMYATT 304

Query: 270 ISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVALRSDSDLSVFFRCR 329
           I+G+ + +DG TTFRSAT A  G+GFI +DI  +NTAGP K QAVALR  +D+SV  RCR
Sbjct: 305 ITGSLNVVDGSTTFRSATLAAVGQGFILQDICIQNTAGPAKDQAVALRVGADMSVINRCR 364

Query: 330 IRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGR 389
           I  YQDTLY H+ RQFYR+  ++GTVDF+FG+A VVFQ C ++A+K    Q+N +TAQGR
Sbjct: 365 IDAYQDTLYAHSQRQFYRDSYVTGTVDFIFGNAAVVFQKCQLVARKPGKYQQNMVTAQGR 424

Query: 390 KDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSFMSDAIRPEGWL 449
            DPNQ TG SIQFCNI A SDL+P +    TYLGRPWKEYSRT++M+S++   I P GW 
Sbjct: 425 TDPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKEYSRTVVMESYLGGLINPAGWA 484

Query: 450 EWNADFALKTLFYAEFMNYGPGAGLAKRVTWPGYRRLNQTSEASNFTVAQFIEGNLWLPS 506
           EW+ DFALKTL+Y EFMN GPGAG +KRV WPGY  +   ++A  FTVA+ I+G  WL S
Sbjct: 485 EWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHVITDPAKAMPFTVAKLIQGGSWLRS 544

BLAST of CmUC10G182530 vs. ExPASy TrEMBL
Match: A0A0A0L4S0 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G000060 PE=3 SV=1)

HSP 1 Score: 933.3 bits (2411), Expect = 4.2e-268
Identity = 464/509 (91.16%), Postives = 490/509 (96.27%), Query Frame = 0

Query: 1   MAKLTAKRLLQFWCFFILLFPM-LSATSPPDLVKIECLTVPASQFSNSILSTIDVVQQVI 60
           MAKLT  + LQF C  ILLFPM L A+SPPDLV+ ECL+VP+SQFSNS+LSTIDVV+QV+
Sbjct: 70  MAKLTT-QTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVM 129

Query: 61  AIFSPFTKLLGDLRLSTAVSDCLDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTW 120
           AIFSPF+KLLGD RLSTA+SDCLDLLDSSADQLSWSLSATQNPKAK+HSTGDLSSDLKTW
Sbjct: 130 AIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTW 189

Query: 121 LSAAVVNPETCMDGFEGTNSIIKGLVSGSINQLTSQLYELLSMVKSIPNQPAADFPSWVK 180
           LSAAVVNPETCMDGFEGTNSIIKGLVSG +NQLTSQLY+LLSMVKSIPNQP ++FPSW+K
Sbjct: 190 LSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQP-SEFPSWLK 249

Query: 181 SEDRNLLQLNDFAADATVAADGTGDFTSVMDAVLAAPDNSIQRYVIYIKRGVYFENVEIK 240
           SED+NLLQ+ND AADATVAADGTGDFT+VMDAVLAAPDNSI+RYVIYIK+GVY ENVEIK
Sbjct: 250 SEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIK 309

Query: 241 KKKWNLMMIGDGIDATTISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQ 300
           KKKWNLMMIGDGIDAT ISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAG +KHQ
Sbjct: 310 KKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQ 369

Query: 301 AVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSIL 360
           AVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSIL
Sbjct: 370 AVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSIL 429

Query: 361 AKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRT 420
           AKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRT
Sbjct: 430 AKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRT 489

Query: 421 IIMQSFMSDAIRPEGWLEWNADFALKTLFYAEFMNYGPGAGLAKRVTWPGYRRLNQTSEA 480
           IIMQS++SDAIRPEGWLEWNA+FAL TLFYAEFMNYGPGAGLAKRV WPGY RLNQTSEA
Sbjct: 490 IIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEA 549

Query: 481 SNFTVAQFIEGNLWLPSTGVKYTAGLGAN 509
           +NFTVAQFIEGNLWLPSTGVKYTAGLGAN
Sbjct: 550 TNFTVAQFIEGNLWLPSTGVKYTAGLGAN 576

BLAST of CmUC10G182530 vs. ExPASy TrEMBL
Match: A0A1S3BKA0 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103490630 PE=3 SV=1)

HSP 1 Score: 931.8 bits (2407), Expect = 1.2e-267
Identity = 464/509 (91.16%), Postives = 488/509 (95.87%), Query Frame = 0

Query: 1   MAKLTAKRLLQFWCFFILLFPM-LSATSPPDLVKIECLTVPASQFSNSILSTIDVVQQVI 60
           MAKLT  ++LQF C FILLFPM L A+SP DLV+ ECL+VP+SQFSNS+LSTIDVV+QV+
Sbjct: 70  MAKLTT-QILQFCCIFILLFPMLLPASSPADLVQTECLSVPSSQFSNSLLSTIDVVRQVM 129

Query: 61  AIFSPFTKLLGDLRLSTAVSDCLDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTW 120
           AIFSPF+KLLGD RLSTA+SDCLDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTW
Sbjct: 130 AIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTW 189

Query: 121 LSAAVVNPETCMDGFEGTNSIIKGLVSGSINQLTSQLYELLSMVKSIPNQPAADFPSWVK 180
           LSAAVVNPETCMDGFEGTNSIIKGLVSG INQLTSQLY+LLSMVKSIPNQP ++FPSW+K
Sbjct: 190 LSAAVVNPETCMDGFEGTNSIIKGLVSGGINQLTSQLYDLLSMVKSIPNQP-SEFPSWLK 249

Query: 181 SEDRNLLQLNDFAADATVAADGTGDFTSVMDAVLAAPDNSIQRYVIYIKRGVYFENVEIK 240
           SEDRNLLQLND AADATVAADGTGDFT+VMDAVLAAPD S +RYVIYIK+GVY ENVEIK
Sbjct: 250 SEDRNLLQLNDLAADATVAADGTGDFTNVMDAVLAAPDKSTRRYVIYIKKGVYLENVEIK 309

Query: 241 KKKWNLMMIGDGIDATTISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQ 300
           KKKWNLMMIGDGIDAT ISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAG +KHQ
Sbjct: 310 KKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQ 369

Query: 301 AVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSIL 360
           AVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSIL
Sbjct: 370 AVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSIL 429

Query: 361 AKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRT 420
           AKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRT
Sbjct: 430 AKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRT 489

Query: 421 IIMQSFMSDAIRPEGWLEWNADFALKTLFYAEFMNYGPGAGLAKRVTWPGYRRLNQTSEA 480
           IIMQS++SDAIRPEGWLEWN +FAL TLFYAEFMNYGPGAGLAKRV WPGY RLNQTSEA
Sbjct: 490 IIMQSYISDAIRPEGWLEWNGNFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEA 549

Query: 481 SNFTVAQFIEGNLWLPSTGVKYTAGLGAN 509
           +NFTVAQFIEGN+WLPSTGVKYTAGLGAN
Sbjct: 550 TNFTVAQFIEGNMWLPSTGVKYTAGLGAN 576

BLAST of CmUC10G182530 vs. ExPASy TrEMBL
Match: A0A5D3CHH9 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00030 PE=3 SV=1)

HSP 1 Score: 911.8 bits (2355), Expect = 1.3e-261
Identity = 448/487 (91.99%), Postives = 471/487 (96.71%), Query Frame = 0

Query: 22  MLSATSPPDLVKIECLTVPASQFSNSILSTIDVVQQVIAIFSPFTKLLGDLRLSTAVSDC 81
           +L A+SP DLV+ ECL+VP+SQFSNS+LSTIDVV+QV+AIFSPF+KLLGD RLSTA+SDC
Sbjct: 2   LLPASSPADLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDC 61

Query: 82  LDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSII 141
           LDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSII
Sbjct: 62  LDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSII 121

Query: 142 KGLVSGSINQLTSQLYELLSMVKSIPNQPAADFPSWVKSEDRNLLQLNDFAADATVAADG 201
           KGLVSG INQLTSQLY+LLSMVKSIPNQP ++FPSW+KSEDRNLLQLND AADATVAADG
Sbjct: 122 KGLVSGGINQLTSQLYDLLSMVKSIPNQP-SEFPSWLKSEDRNLLQLNDLAADATVAADG 181

Query: 202 TGDFTSVMDAVLAAPDNSIQRYVIYIKRGVYFENVEIKKKKWNLMMIGDGIDATTISGNR 261
           TGDFT+VMDAVLAAPD S +RYVIYIK+GVY ENVEIKKKKWNLMMIGDGIDAT ISGNR
Sbjct: 182 TGDFTNVMDAVLAAPDKSTRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNR 241

Query: 262 SFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVALRSDSDLSVFFRCRIRGYQ 321
           SFIDGWTTFRSATFAVSGRGFIARDITFENTAG +KHQAVALRSDSDLSVFFRCRIRGYQ
Sbjct: 242 SFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQ 301

Query: 322 DTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQ 381
           DTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQ
Sbjct: 302 DTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQ 361

Query: 382 PTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSFMSDAIRPEGWLEWNAD 441
           PTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQS++SDAIRPEGWLEWN +
Sbjct: 362 PTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNGN 421

Query: 442 FALKTLFYAEFMNYGPGAGLAKRVTWPGYRRLNQTSEASNFTVAQFIEGNLWLPSTGVKY 501
           FAL TLFYAEFMNYGPGAGLAKRV WPGY RLNQTSEA+NFTVAQFIEGN+WLPSTGVKY
Sbjct: 422 FALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNMWLPSTGVKY 481

Query: 502 TAGLGAN 509
           TAGLGAN
Sbjct: 482 TAGLGAN 487

BLAST of CmUC10G182530 vs. ExPASy TrEMBL
Match: A0A6J1GCJ9 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111452924 PE=3 SV=1)

HSP 1 Score: 871.7 bits (2251), Expect = 1.5e-249
Identity = 432/513 (84.21%), Postives = 473/513 (92.20%), Query Frame = 0

Query: 1   MAKLTAKRLLQFWCFFIL-LFPMLSATSPPDLVKIECLTVPASQFSNSILSTIDVVQQVI 60
           MAKLT K  L+FW F +L LFPM  A    DL + ECL VP+S+FSNS+LSTI VV+QV+
Sbjct: 1   MAKLT-KTHLKFWLFMLLILFPMAPAA---DLEQTECLKVPSSEFSNSVLSTIGVVRQVM 60

Query: 61  AIFSPFTKLLGDLRLSTAVSDCLDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTW 120
            IFSPFTK LGD RLSTA+SDCLDLLDSS+DQLSWSLSA QNPKAK++STGDLSSDLKTW
Sbjct: 61  LIFSPFTKFLGDSRLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTW 120

Query: 121 LSAAVVNPETCMDGFEGTNSIIKGLVSGSINQLTSQLYELLSMVKSIPNQPA----ADFP 180
           LSAA VNPETCM+GFEGTNSI+KGLVS  INQLTSQ+Y LLSMVKSI NQP     ++FP
Sbjct: 121 LSAAGVNPETCMEGFEGTNSIVKGLVSEGINQLTSQVYNLLSMVKSISNQPVGKGESEFP 180

Query: 181 SWVKSEDRNLLQLNDFAADATVAADGTGDFTSVMDAVLAAPDNSIQRYVIYIKRGVYFEN 240
           +W+K+E+++LLQ NDFAADATVAADGTGDFT+VM+AVLAAPD S++RYVIY+K+GVYFEN
Sbjct: 181 AWLKTEEQSLLQQNDFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFEN 240

Query: 241 VEIKKKKWNLMMIGDGIDATTISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGP 300
           VEIKKKKWN+MMIGDGIDAT ISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGP
Sbjct: 241 VEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGP 300

Query: 301 QKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQN 360
           +KHQAVALRSDSDLSVFFRCR+RGYQDTLYTHTMRQFYREC ISGTVDFLFGDATVVFQN
Sbjct: 301 EKHQAVALRSDSDLSVFFRCRMRGYQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQN 360

Query: 361 CSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKE 420
           C+ILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDL+PSVN TATYLGRPWKE
Sbjct: 361 CTILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLQPSVNATATYLGRPWKE 420

Query: 421 YSRTIIMQSFMSDAIRPEGWLEWNADFALKTLFYAEFMNYGPGAGLAKRVTWPGYRRLNQ 480
           YSRTIIMQS++S+AIRPEGWLEWN DFAL TLFYAEFMNYGPGAGLAKRV WPGY RLNQ
Sbjct: 421 YSRTIIMQSYVSNAIRPEGWLEWNGDFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQ 480

Query: 481 TSEASNFTVAQFIEGNLWLPSTGVKYTAGLGAN 509
           TSEA+NFTV+QFIEGNLWLPSTGVK+T+G GAN
Sbjct: 481 TSEATNFTVSQFIEGNLWLPSTGVKFTSGFGAN 509

BLAST of CmUC10G182530 vs. ExPASy TrEMBL
Match: A0A6J1I863 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470062 PE=3 SV=1)

HSP 1 Score: 868.2 bits (2242), Expect = 1.7e-248
Identity = 433/514 (84.24%), Postives = 473/514 (92.02%), Query Frame = 0

Query: 1   MAKLTAKRLLQFWCFFIL-LFPMLSATSPPDLVKIECLTVPASQFSNSILSTIDVVQQVI 60
           MAKLT K  L+FW F +L LFP+  A    DL + ECL VP+S+FSNS+LSTI VV+QV+
Sbjct: 89  MAKLT-KTHLKFWLFMLLILFPLAPAA---DLEQTECLKVPSSEFSNSVLSTIGVVRQVM 148

Query: 61  AIFSPFTKLLGDLRLSTAVSDCLDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTW 120
            IFSPFTK LGD RLSTA+SDCLDLLDSS+DQLSWSLSA QNPKAK++STGDLSSDLKTW
Sbjct: 149 LIFSPFTKFLGDSRLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTW 208

Query: 121 LSAAVVNPETCMDGFEGTNSIIKGLVSGSINQLTSQLYELLSMVKSIPNQPA----ADFP 180
           LSAA VNPETCM+GFEGTNSI+KGLVS  INQLTSQ+Y LLSMVKSI NQPA    + FP
Sbjct: 209 LSAAGVNPETCMEGFEGTNSIVKGLVSEGINQLTSQVYNLLSMVKSISNQPAGKGESGFP 268

Query: 181 SWVKSEDRNLLQLNDFAADATVAADGTGDFTSVMDAVLAAPDNSIQRYVIYIKRGVYFEN 240
           +W+KSE+++LLQ NDFAADATVAADGTGDFT+VM+AVLAAPD S++RYVIY+K+GVYFEN
Sbjct: 269 AWLKSEEQSLLQQNDFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFEN 328

Query: 241 VEIKKKKWNLMMIGDGIDATTISGNRSFIDGWTTFRSATFAVSG-RGFIARDITFENTAG 300
           VEIKKKKWN+MMIGDGIDAT ISGNRSFIDGWTTFRSATFAVSG RGFIARDITFENTAG
Sbjct: 329 VEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATFAVSGRRGFIARDITFENTAG 388

Query: 301 PQKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQ 360
           P+KHQAVALRSDSDLSVFFRCR+ GYQDTLYTHTMRQFYREC ISGTVDFLFGDATVVFQ
Sbjct: 389 PEKHQAVALRSDSDLSVFFRCRMSGYQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQ 448

Query: 361 NCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWK 420
           NC+ILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDL+PSVNTTATYLGRPWK
Sbjct: 449 NCTILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLQPSVNTTATYLGRPWK 508

Query: 421 EYSRTIIMQSFMSDAIRPEGWLEWNADFALKTLFYAEFMNYGPGAGLAKRVTWPGYRRLN 480
           EYSRTIIMQS+MS+AIRPEGWLEWN DFAL TLFYAEFMNYGPGAGLAKR+ WPGY RLN
Sbjct: 509 EYSRTIIMQSYMSNAIRPEGWLEWNGDFALNTLFYAEFMNYGPGAGLAKRINWPGYHRLN 568

Query: 481 QTSEASNFTVAQFIEGNLWLPSTGVKYTAGLGAN 509
           QTSEA+NFTV+QFIEGNLWLPSTGVK+T+G GAN
Sbjct: 569 QTSEATNFTVSQFIEGNLWLPSTGVKFTSGFGAN 598

BLAST of CmUC10G182530 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 671.8 bits (1732), Expect = 4.4e-193
Identity = 327/525 (62.29%), Postives = 412/525 (78.48%), Query Frame = 0

Query: 1   MAKLTAKRLLQFWCFFILL---FPMLSATSPPDLVKIECLTVPASQFSNSILSTIDVVQQ 60
           MAK        F+ F I++        A S  DLV++ECL VP  +F+ +  + +D + +
Sbjct: 1   MAKFRQMGSSIFFLFLIIISLCSAHKEAFSSTDLVQMECLRVPPLEFAEAAKTVVDAITK 60

Query: 61  VIAIFSPFTKLLGDLRLSTAVSDCLDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLK 120
            +AI S F K  G  R+S A+ DC+DLLDS+A++LSW +SA+Q+P  KD+STGD+ SDL+
Sbjct: 61  AVAIVSKFDKKAGKSRVSNAIVDCVDLLDSAAEELSWIISASQSPNGKDNSTGDVGSDLR 120

Query: 121 TWLSAAVVNPETCMDGFEGTNSIIKGLVSGSINQLTSQLYELLSMVKSIPNQPA------ 180
           TW+SAA+ N +TC+DGFEGTN IIK +V+G ++++ + +  LL+MV S P++P       
Sbjct: 121 TWISAALSNQDTCLDGFEGTNGIIKKIVAGGLSKVGTTVRNLLTMVHSPPSKPKPKPIKA 180

Query: 181 ----------ADFPSWVKSEDRNLLQLNDF-AADATVAADGTGDFTSVMDAVLAAPDNSI 240
                     + FPSWVK  DR LLQ ++   ADA VAADGTG+FT++ DAVLAAPD S 
Sbjct: 181 QTMTKAHSGFSKFPSWVKPGDRKLLQTDNITVADAVVAADGTGNFTTISDAVLAAPDYST 240

Query: 241 QRYVIYIKRGVYFENVEIKKKKWNLMMIGDGIDATTISGNRSFIDGWTTFRSATFAVSGR 300
           +RYVI++KRGVY ENVEIKKKKWN+MM+GDGIDAT I+GNRSFIDGWTTFRSATFAVSGR
Sbjct: 241 KRYVIHVKRGVYVENVEIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGR 300

Query: 301 GFIARDITFENTAGPQKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISG 360
           GFIARDITF+NTAGP+KHQAVA+RSD+DL VF+RC +RGYQDTLY H+MRQF+REC I+G
Sbjct: 301 GFIARDITFQNTAGPEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITG 360

Query: 361 TVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKP 420
           TVDF+FGDAT VFQ+C I AK+GLPNQKN+ITAQGRKDPN+PTGF+IQF NI+AD+DL  
Sbjct: 361 TVDFIFGDATAVFQSCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLL 420

Query: 421 SVNTTATYLGRPWKEYSRTIIMQSFMSDAIRPEGWLEWNADFALKTLFYAEFMNYGPGAG 480
           ++NTTATYLGRPWK YSRT+ MQ++MSDAI P GWLEWN +FAL TL+Y E+MN GPGA 
Sbjct: 421 NLNTTATYLGRPWKLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGAS 480

Query: 481 LAKRVTWPGYRRLNQTSEASNFTVAQFIEGNLWLPSTGVKYTAGL 506
           L +RV WPGY  LN ++EA+NFTV+Q I+GNLWLPSTG+ + AGL
Sbjct: 481 LDRRVKWPGYHVLNTSAEANNFTVSQLIQGNLWLPSTGITFIAGL 525

BLAST of CmUC10G182530 vs. TAIR 10
Match: AT4G33220.1 (pectin methylesterase 44 )

HSP 1 Score: 671.8 bits (1732), Expect = 4.4e-193
Identity = 328/518 (63.32%), Postives = 400/518 (77.22%), Query Frame = 0

Query: 15  FFILLFPMLSATSPPDLVKIECLTVPASQFSNSILSTIDVVQQVIAIFSPFTKLLGDLRL 74
           +F++L  +    S  +  + + L VPAS+F +SI + + V++QV +I S F    GD RL
Sbjct: 6   YFLILLMLGLCVSSEENFQYDYLKVPASEFVSSINTIVVVIRQVSSILSQFADFSGDRRL 65

Query: 75  STAVSDCLDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTWLSAAVVNPETCMDGF 134
             AVSDCLDLLD S+++L+WS SA++NPK K + TGD+ SD +TWLSAA+ N  TCM+GF
Sbjct: 66  QNAVSDCLDLLDFSSEELTWSASASENPKGKGNGTGDVGSDTRTWLSAALSNQATCMEGF 125

Query: 135 EGTNSIIKGLVSGSINQLTSQLYELLSMV-------------------KSIPNQPAAD-- 194
           +GT+ ++K LV+GS++QL S L ELL +V                   K+ P +   D  
Sbjct: 126 DGTSGLVKSLVAGSLDQLYSMLRELLPLVQPEQKPKAVSKPGPIAKGPKAPPGRKLRDTD 185

Query: 195 ------FPSWVKSEDRNLLQLNDFAADATVAADGTGDFTSVMDAVLAAPDNSIQRYVIYI 254
                 FP WV+ +DR LL+ N    D +VA DGTG+FT +MDA+  APD S  R+VIYI
Sbjct: 186 EDESLQFPDWVRPDDRKLLESNGRTYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYI 245

Query: 255 KRGVYFENVEIKKKKWNLMMIGDGIDATTISGNRSFIDGWTTFRSATFAVSGRGFIARDI 314
           K+G+Y ENVEIKKKKWN++M+GDGID T ISGNRSFIDGWTTFRSATFAVSGRGF+ARDI
Sbjct: 246 KKGLYLENVEIKKKKWNIVMLGDGIDVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDI 305

Query: 315 TFENTAGPQKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFG 374
           TF+NTAGP+KHQAVALRSDSDLSVFFRC +RGYQDTLYTHTMRQFYREC I+GTVDF+FG
Sbjct: 306 TFQNTAGPEKHQAVALRSDSDLSVFFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFG 365

Query: 375 DATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTAT 434
           D TVVFQNC ILAK+GLPNQKNTITAQGRKD NQP+GFSIQF NISAD+DL P +NTT T
Sbjct: 366 DGTVVFQNCQILAKRGLPNQKNTITAQGRKDVNQPSGFSIQFSNISADADLVPYLNTTRT 425

Query: 435 YLGRPWKEYSRTIIMQSFMSDAIRPEGWLEWNADFALKTLFYAEFMNYGPGAGLAKRVTW 494
           YLGRPWK YSRT+ +++ MSD +RPEGWLEWNADFAL TLFY EFMNYGPG+GL+ RV W
Sbjct: 426 YLGRPWKLYSRTVFIRNNMSDVVRPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKW 485

Query: 495 PGYRRLNQTSEASNFTVAQFIEGNLWLPSTGVKYTAGL 506
           PGY   N + +A+NFTV+QFI+GNLWLPSTGV ++ GL
Sbjct: 486 PGYHVFNNSDQANNFTVSQFIKGNLWLPSTGVTFSDGL 523

BLAST of CmUC10G182530 vs. TAIR 10
Match: AT3G05620.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 430.3 bits (1105), Expect = 2.2e-120
Identity = 217/452 (48.01%), Postives = 291/452 (64.38%), Query Frame = 0

Query: 73  RLSTAVSDCLDLLDSSADQLSWSLSATQNPKA--------KDHSTGDLSSDLKTWLSAAV 132
           R   A+ DC +L+  S  +L+WS+                  H       +LKTWLSAA+
Sbjct: 100 REQVAIEDCKELVGFSVTELAWSMLEMNKLHGGGGIDLDDGSHDAAAAGGNLKTWLSAAM 159

Query: 133 VNPETCMDGFEGTNSIIKGLVSGSINQLTSQLYELLSM--------VKSIPNQPAADFPS 192
            N +TC++GFEGT    + L+ GS+ Q+T  +  +L M         K+  N+     P 
Sbjct: 160 SNQDTCLEGFEGTERKYEELIKGSLRQVTQLVSNVLDMYTQLNALPFKASRNESVIASPE 219

Query: 193 WVKSEDRNLLQLND---FAADATVAADGTGDFTSVMDAVLAAPDNSIQRYVIYIKRGVYF 252
           W+   D +L+  +D      +  VA DG G + ++ +A+  AP++S +RYVIY+K+GVY 
Sbjct: 220 WLTETDESLMMRHDPSVMHPNTVVAIDGKGKYRTINEAINEAPNHSTKRYVIYVKKGVYK 279

Query: 253 ENVEIKKKKWNLMMIGDGIDATTISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTA 312
           EN+++KKKK N+M++GDGI  T I+G+R+F+ G TTFR+AT AVSGRGFIA+DITF NTA
Sbjct: 280 ENIDLKKKKTNIMLVGDGIGQTIITGDRNFMQGLTTFRTATVAVSGRGFIAKDITFRNTA 339

Query: 313 GPQKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVF 372
           GPQ  QAVALR DSD S F+RC + GYQDTLY H++RQFYR+C+I GT+DF+FG+   V 
Sbjct: 340 GPQNRQAVALRVDSDQSAFYRCSVEGYQDTLYAHSLRQFYRDCEIYGTIDFIFGNGAAVL 399

Query: 373 QNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPW 432
           QNC I  +  LP QK TITAQGRK PNQ TGF IQ   + A         T  TYLGRPW
Sbjct: 400 QNCKIYTRVPLPLQKVTITAQGRKSPNQNTGFVIQNSYVLA---------TQPTYLGRPW 459

Query: 433 KEYSRTIIMQSFMSDAIRPEGWLEWNADFALKTLFYAEFMNYGPGAGLAKRVTWPGYRRL 492
           K YSRT+ M ++MS  ++P GWLEW  +FAL TL+Y E+ N GPG   + RV WPGY  +
Sbjct: 460 KLYSRTVYMNTYMSQLVQPRGWLEWFGNFALDTLWYGEYNNIGPGWRSSGRVKWPGYHIM 519

Query: 493 NQTSEASNFTVAQFIEGNLWLPSTGVKYTAGL 506
           ++ + A +FTV  FI+G  WLP+TGV +TAGL
Sbjct: 520 DKRT-ALSFTVGSFIDGRRWLPATGVTFTAGL 541

BLAST of CmUC10G182530 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 426.0 bits (1094), Expect = 4.2e-119
Identity = 225/456 (49.34%), Postives = 294/456 (64.47%), Query Frame = 0

Query: 73  RLSTAVSDCLDLLDSSADQLSWSLSATQNPKAKDHSTGDLS-SDLKTWLSAAVVNPETCM 132
           R+ +A   CL+LLD S D L+ +LS+          +GD S SD+ TWLS+A+ N +TC 
Sbjct: 136 RVRSAYDSCLELLDDSVDALTRALSSVV------VVSGDESHSDVMTWLSSAMTNHDTCT 195

Query: 133 DGF---EGTNSIIKGLVSGSINQLTSQLYELLSM----VKSIPNQPAA------------ 192
           DGF   EG    +K  V G++  L+  +   L++    VK +   P              
Sbjct: 196 DGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETE 255

Query: 193 DFPSWVKSEDRNLL--QLNDFAADATVAADGTGDFTSVMDAVLAAPDNSIQRYVIYIKRG 252
           + P+W+K EDR LL    +   AD TV+ DG+G F ++ +A+  AP++S +R+VIY+K G
Sbjct: 256 ELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKAG 315

Query: 253 VY-FENVEIKKKKWNLMMIGDGIDATTISGNRSFIDGWTTFRSATFAVSGRGFIARDITF 312
            Y  EN+++ +KK NLM IGDG   T I+G +S  D  TTF +ATFA +G GFI RD+TF
Sbjct: 316 RYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVRDMTF 375

Query: 313 ENTAGPQKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDA 372
           EN AGP KHQAVALR   D +V +RC I GYQD LY H+ RQF+REC+I GTVDF+FG+A
Sbjct: 376 ENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGNA 435

Query: 373 TVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYL 432
            V+ Q+C+I A+K +  QK TITAQ RKDPNQ TG SI  C + A  DL+ S  +  TYL
Sbjct: 436 AVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPTYL 495

Query: 433 GRPWKEYSRTIIMQSFMSDAIRPEGWLEWNADFALKTLFYAEFMNYGPGAGLAKRVTWPG 492
           GRPWK YSR + M S M D I P GWLEWN  FAL +L+Y E+MN G G+G+ +RV WPG
Sbjct: 496 GRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRVKWPG 555

Query: 493 YRRLNQTSEASNFTVAQFIEGNLWLPSTGVKYTAGL 506
           Y  +  T EAS FTVAQFI G+ WLPSTGV + +GL
Sbjct: 556 YHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 585

BLAST of CmUC10G182530 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 424.9 bits (1091), Expect = 9.4e-119
Identity = 228/507 (44.97%), Postives = 316/507 (62.33%), Query Frame = 0

Query: 29  PDLVKIECLTVPASQFSNSILSTIDVVQQVI------AIFSPFTKLLGDL--RLSTAVSD 88
           P+L     +  P S  ++S    I V   +       A++S  +    D+  R  +A   
Sbjct: 97  PELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSASLSFVDMPPRARSAYDS 156

Query: 89  CLDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTWLSAAVVNPETCMDGFEGT-NS 148
           C++LLD S D LS +LS+  +  AK         D+ TWLSAA+ N +TC +GF+G  + 
Sbjct: 157 CVELLDDSVDALSRALSSVVSSSAK-------PQDVTTWLSAALTNHDTCTEGFDGVDDG 216

Query: 149 IIKGLVSGSINQLTSQLYELLSMVKS---------IP---------NQPAADFPSWVKSE 208
            +K  ++ ++  L+  +   L++  +         +P          +    FP W++ +
Sbjct: 217 GVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLGVEEREEKFPRWMRPK 276

Query: 209 DRNLLQL--NDFAADATVAADGTGDFTSVMDAVLAAPDNSIQRYVIYIKRGVYFE-NVEI 268
           +R +L++  +   AD  V+ DG G   ++ +A+  AP NS +R +IY+K G Y E N+++
Sbjct: 277 EREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAGRYEENNLKV 336

Query: 269 KKKKWNLMMIGDGIDATTISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPQKH 328
            +KK NLM +GDG   T ISG +S  D  TTF +A+FA +G GFIARDITFEN AGP KH
Sbjct: 337 GRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFIARDITFENWAGPAKH 396

Query: 329 QAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSI 388
           QAVALR  +D +V +RC I GYQDTLY H+ RQF+REC I GTVDF+FG+A VV QNCSI
Sbjct: 397 QAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSI 456

Query: 389 LAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSR 448
            A+K +  QKNTITAQ RKDPNQ TG SI    + A SDL+ +  +T TYLGRPWK +SR
Sbjct: 457 YARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQATNGSTQTYLGRPWKLFSR 516

Query: 449 TIIMQSFMSDAIRPEGWLEWNADFALKTLFYAEFMNYGPGAGLAKRVTWPGYRRLNQTSE 506
           T+ M S++   +   GWLEWN  FAL TL+Y E++N GPG+GL +RV+WPGYR +N T+E
Sbjct: 517 TVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAE 576

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004146188.28.7e-26891.16pectinesterase/pectinesterase inhibitor PPE8B [Cucumis sativus] >XP_031738323.1 ... [more]
XP_008448427.12.5e-26791.16PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis melo][more]
XP_038905308.11.2e-26189.39pectinesterase/pectinesterase inhibitor PPE8B-like [Benincasa hispida][more]
KAA0052899.12.7e-26191.99pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis melo var. makuwa] >T... [more]
XP_022949597.13.1e-24984.21pectinesterase/pectinesterase inhibitor PPE8B-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q430621.1e-20971.46Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=... [more]
Q9LXK76.2e-19262.29Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3... [more]
Q9SMY76.2e-19263.32Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3... [more]
Q965752.4e-12748.26Pectinesterase 2.2 OS=Solanum lycopersicum OX=4081 GN=PME2.2 PE=3 SV=1[more]
P142804.1e-12748.67Pectinesterase 1 OS=Solanum lycopersicum OX=4081 GN=PME1.9 PE=1 SV=5[more]
Match NameE-valueIdentityDescription
A0A0A0L4S04.2e-26891.16Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G000060 PE=3 SV=1[more]
A0A1S3BKA01.2e-26791.16Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103490630 PE=3 SV=1[more]
A0A5D3CHH91.3e-26191.99Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00030... [more]
A0A6J1GCJ91.5e-24984.21Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111452924 PE=3 SV=1[more]
A0A6J1I8631.7e-24884.24Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470062 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G43270.14.4e-19362.29Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G33220.14.4e-19363.32pectin methylesterase 44 [more]
AT3G05620.12.2e-12048.01Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G53370.14.2e-11949.34pectin methylesterase PCR fragment F [more]
AT3G49220.19.4e-11944.97Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 27..166
e-value: 5.7E-11
score: 52.5
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 75..162
e-value: 2.1E-14
score: 54.1
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 27..169
e-value: 6.9E-15
score: 57.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 47..164
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 188..505
e-value: 1.8E-123
score: 414.0
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 194..491
e-value: 8.9E-149
score: 494.1
NoneNo IPR availablePANTHERPTHR31707:SF278PECTINESTERASE/PECTINESTERASE INHIBITOR 44-RELATEDcoord: 38..165
coord: 171..505
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 38..165
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 171..505
NoneNo IPR availableCDDcd15799PMEI-like_4coord: 14..167
e-value: 1.30192E-42
score: 146.786
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 192..504

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmUC10G182530.1CmUC10G182530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity