CmUC08G154850 (gene) Watermelon (USVL531) v1

Overview


Name	CmUC08G154850
Type	gene
Organism	Citrullus mucosospermus (Watermelon (USVL531) v1)
Description	gibberellin 3-beta-dioxygenase 1-like
Location	CmU531Chr08: 24232371 .. 24234422 (+)
RNA-Seq Expression	CmUC08G154850
Synteny	CmUC08G154850

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGATCAAGAGCTTCCTCCCCCAAGCCAACTTGTTCCTTTGGACTTCAACTCAATTCAAACTGTCCCTGAATCTCACTTGTGGCCAAACTCCATTGAGTTATCGCCCGCAATCAAGTTGGACGAAAGGATTTCGCTCCCGATAATCGACTTTCTCGACGATGGTGCGTCGGAGCTCCTAGGGAGGGCATGTGAGCAATGGGGAATGTTTCAGTTGATTAACCATGGTGTCCCGAAAACCCTAATCGACGAAGCGGAAGAGGAGACTCGACGTCTTTTCTCTCTCCCGGCTAGACAGAAGATGAAGACATTACGGACAGACAGCCTCACCGCCACTGGCTATGGCATGGCCGGAATATCGAAGTATCATCAGAAATTAATGTGGCATGAAGGATTCACTATGTCCGGTTCTCCGGTTGATGATGCTAAGAAGCTTTGGCCAAATGACTACAAACGGTTTTGGTAAATCCATTCAACTTTGTCATGCATGCATGTTGTCCACACTTAGGGTTTATCATCATAACATATTCATTAAAGGAAACAACTTGGGTGCTTCTGGACTGCGCCTATCTTTTGTGCATGCAACCCAGGTGGCATCCAATCAAATTGTGTTATGTGATAAAAAAAAAAATTTAAAAATTAAATTTTTTAATTTCATTTTTTTTAAAAAAAAGAGAGTAGTATTTGATTAGGTACCACCTAGTCATTGCCCTAAATTAAATTGTTAGGTAATTTTAATTTCATGCGTATAAATTAGAGTTAAATTATCGTAATATATATAGAGTTTCAGTCATTATCATTTAAATTTGGTTAAATTTTTAAACATGGGGTCGTCTCTCATTAAATTGGGATGATTCATCATCGTGTTATCTTCTATTTTTCTTTGTATTTAGAAAAAATACATAACAAATTTAAGTTGATTTTAAATAAAATCAAGGAAAATAGATATAAATCATTAATAGTCTAAACTATGTTTCACTCTTGAAATTTTAAGCCTTAAATTGATATAATAATTTAGACATTTTAATTAATGCAATCTTTAAAGTTTAGAGAGATATATTAAAAAAAAAAAAAGTTTCTACTTATTAATTATTCTTTAACAAATTTTAATTAAAAAAATGGGCTAGTTTTAAGATTTATGACTTTTTTTTTTTTATAAATAAATAAATTAATAATAAAAAGAGACTAAATTAATTTGGGACCCATACCTCAAGTCTATATTACAAAATTGAAAAAAGGAAAATTTGCTTAAATTGTTTATGTTTTAAATCTCCCATTTCTGTTTCGATTATAGAAATTCCTTGCTTTATTATCTGATCACACTTCATATATATATATATATATATATATATATAAGTAAGTAAAATTTACATGCACACTAACAATTAATATATGCCCTAATTGAAAAGTTTCTACTCATATATTATTGTATTGTAACCTTTTTTTTTTAAAAAAAAAAAAAAGTGATGTAATGGAAGAATATCAAAGCCAAATGAAGGGTTTAGGAGAAAGGATAATTAGGTTAATCTTCAAGTTTCTTGACATCTCCGAGGAAGAGATGATGAAGCTGTTGACTTCAACCGACGAGTCCGCCGGGAAACCCTACATGGCCCTCCGCTTGAACTCGTATCCACCGTGCCCCGATCCAAGTCGAGTCATGGGCCTCGCCGCCCACACCGACACCTCCCTCTTCACCATCGTTCATCAGGCACGCCATGACGGGCTCCAAGTCTTTAAAGACGGAACAGGCTGGGTTCCGTTGTCTCCGATGAGCGGTACTCTCATCGTCAACGTCGGCGATATCCTACATATTCTGTCAAACGGGCGGTTTCCGAGCATTCTTCACCGGGTGATGATACACGAAAATAAGGAGCATAGATTTTCATTGGCATATTTCTATGATCCACCAGGTGATTTATACATTTCACCTTATTGTAAGCCATTGAGTGAATCTCCACAAATTCCTCTTTATAGATGTGTGAATGTGAAGGAATATTTCACCATCAAGGCCAAGAAAGAGGGGAAAGGACTTTCTGCCATCAAAATATGA

mRNA sequence

ATGGCAGATCAAGAGCTTCCTCCCCCAAGCCAACTTGTTCCTTTGGACTTCAACTCAATTCAAACTGTCCCTGAATCTCACTTGTGGCCAAACTCCATTGAGTTATCGCCCGCAATCAAGTTGGACGAAAGGATTTCGCTCCCGATAATCGACTTTCTCGACGATGGTGCGTCGGAGCTCCTAGGGAGGGCATGTGAGCAATGGGGAATGTTTCAGTTGATTAACCATGGTGTCCCGAAAACCCTAATCGACGAAGCGGAAGAGGAGACTCGACGTCTTTTCTCTCTCCCGGCTAGACAGAAGATGAAGACATTACGGACAGACAGCCTCACCGCCACTGGCTATGGCATGGCCGGAATATCGAAGTATCATCAGAAATTAATGTGGCATGAAGGATTCACTATGTCCGGTTCTCCGGTTGATGATGCTAAGAAGCTTTGGCCAAATGACTACAAACGGTTTTGTGATGTAATGGAAGAATATCAAAGCCAAATGAAGGGTTTAGGAGAAAGGATAATTAGGTTAATCTTCAAGTTTCTTGACATCTCCGAGGAAGAGATGATGAAGCTGTTGACTTCAACCGACGAGTCCGCCGGGAAACCCTACATGGCCCTCCGCTTGAACTCGTATCCACCGTGCCCCGATCCAAGTCGAGTCATGGGCCTCGCCGCCCACACCGACACCTCCCTCTTCACCATCGTTCATCAGGCACGCCATGACGGGCTCCAAGTCTTTAAAGACGGAACAGGCTGGGTTCCGTTGTCTCCGATGAGCGGTACTCTCATCGTCAACGTCGGCGATATCCTACATATTCTGTCAAACGGGCGGTTTCCGAGCATTCTTCACCGGGTGATGATACACGAAAATAAGGAGCATAGATTTTCATTGGCATATTTCTATGATCCACCAGGTGATTTATACATTTCACCTTATTGTAAGCCATTGAGTGAATCTCCACAAATTCCTCTTTATAGATGTGTGAATGTGAAGGAATATTTCACCATCAAGGCCAAGAAAGAGGGGAAAGGACTTTCTGCCATCAAAATATGA

Coding sequence (CDS)

ATGGCAGATCAAGAGCTTCCTCCCCCAAGCCAACTTGTTCCTTTGGACTTCAACTCAATTCAAACTGTCCCTGAATCTCACTTGTGGCCAAACTCCATTGAGTTATCGCCCGCAATCAAGTTGGACGAAAGGATTTCGCTCCCGATAATCGACTTTCTCGACGATGGTGCGTCGGAGCTCCTAGGGAGGGCATGTGAGCAATGGGGAATGTTTCAGTTGATTAACCATGGTGTCCCGAAAACCCTAATCGACGAAGCGGAAGAGGAGACTCGACGTCTTTTCTCTCTCCCGGCTAGACAGAAGATGAAGACATTACGGACAGACAGCCTCACCGCCACTGGCTATGGCATGGCCGGAATATCGAAGTATCATCAGAAATTAATGTGGCATGAAGGATTCACTATGTCCGGTTCTCCGGTTGATGATGCTAAGAAGCTTTGGCCAAATGACTACAAACGGTTTTGTGATGTAATGGAAGAATATCAAAGCCAAATGAAGGGTTTAGGAGAAAGGATAATTAGGTTAATCTTCAAGTTTCTTGACATCTCCGAGGAAGAGATGATGAAGCTGTTGACTTCAACCGACGAGTCCGCCGGGAAACCCTACATGGCCCTCCGCTTGAACTCGTATCCACCGTGCCCCGATCCAAGTCGAGTCATGGGCCTCGCCGCCCACACCGACACCTCCCTCTTCACCATCGTTCATCAGGCACGCCATGACGGGCTCCAAGTCTTTAAAGACGGAACAGGCTGGGTTCCGTTGTCTCCGATGAGCGGTACTCTCATCGTCAACGTCGGCGATATCCTACATATTCTGTCAAACGGGCGGTTTCCGAGCATTCTTCACCGGGTGATGATACACGAAAATAAGGAGCATAGATTTTCATTGGCATATTTCTATGATCCACCAGGTGATTTATACATTTCACCTTATTGTAAGCCATTGAGTGAATCTCCACAAATTCCTCTTTATAGATGTGTGAATGTGAAGGAATATTTCACCATCAAGGCCAAGAAAGAGGGGAAAGGACTTTCTGCCATCAAAATATGA

Protein sequence

MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASELLGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGISKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFLDISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHDGLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFYDPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI

Homology

[Close]

BLAST of CmUC08G154850 vs. NCBI nr
Match: AAF80661.1 (putative gibberellin 3 beta hydroxylase [Citrullus lanatus subsp. vulgaris])

HSP 1 Score: 723.0 bits (1865), Expect = 1.2e-204
Identity = 349/349 (100.00%), Postives = 349/349 (100.00%), Query Frame = 0


Query: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60
           MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL
Sbjct: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60

Query: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGI 120
           LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGI
Sbjct: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGI 120

Query: 121 SKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFL 180
           SKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFL
Sbjct: 121 SKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFL 180

Query: 181 DISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHD 240
           DISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHD
Sbjct: 181 DISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHD 240

Query: 241 GLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFY 300
           GLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFY
Sbjct: 241 GLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFY 300

Query: 301 DPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 350
           DPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI
Sbjct: 301 DPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 349

[Close]

BLAST of CmUC08G154850 vs. NCBI nr
Match: NP_001275523.1 (gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus] >KAE8652189.1 hypothetical protein Csa_021993 [Cucumis sativus] >CCG14217.1 GA 3-oxidase [Cucumis sativus])

HSP 1 Score: 627.5 bits (1617), Expect = 7.0e-176
Identity = 296/350 (84.57%), Postives = 325/350 (92.86%), Query Frame = 0


Query: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60
           MADQE+PPPSQLVPLDF S+QTVPESHLWPN+ ELS AIKLD+R+SLP+ID + DGASEL
Sbjct: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60

Query: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRT-DSLTATGYGMAG 120
           LGRACE+WGMFQLINHG+ KTLI+EAEEETRRLF+LPA QKMKTLR+  SLTATGYGMAG
Sbjct: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120

Query: 121 ISKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKF 180
           ISKYH KLMWHEGFT+ GSP+++AKKLWPNDYKRFCDVMEEYQ +MKGLGERIIRLI KF
Sbjct: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180

Query: 181 LDISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARH 240
           L ISEEEMMKLLTSTDES GKP+MALRLNSYPPCPDP +VMGLAAHTDTSL TI+HQ  +
Sbjct: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240

Query: 241 DGLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYF 300
           +GLQ+FKDGTGWVPLSPMSGTLIVN+GD+LHILSNGRFPSILHRVMI ENKEHRFSLAYF
Sbjct: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300

Query: 301 YDPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 350
           YDPPG++YISPYCKPLS++PQ PLYR VNVKEYF IKAKK GKGL AIKI
Sbjct: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 350

[Close]

BLAST of CmUC08G154850 vs. NCBI nr
Match: XP_038885582.1 (gibberellin 3-beta-dioxygenase 1-like [Benincasa hispida])

HSP 1 Score: 626.3 bits (1614), Expect = 1.6e-175
Identity = 298/347 (85.88%), Postives = 322/347 (92.80%), Query Frame = 0


Query: 3   DQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASELLG 62
           DQELPPPSQ VPLDF+SIQTVPESHLWP+S ELS A++LDER+SLPIID LD+GASELLG
Sbjct: 5   DQELPPPSQFVPLDFSSIQTVPESHLWPDSNELSLAVELDERVSLPIIDLLDNGASELLG 64

Query: 63  RACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGISK 122
           RACE+WGMFQLINHGVPKTLIDEAEEETRRLF+LPARQKMK L++ S + TGYGMAGISK
Sbjct: 65  RACEEWGMFQLINHGVPKTLIDEAEEETRRLFTLPARQKMKALQSPS-SITGYGMAGISK 124

Query: 123 YHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFLDI 182
           YH KLMWHEGFT+ GSPVDDAKKLWPNDYKRFCDVMEEYQ+QMKGLGER+IRLIFKFL I
Sbjct: 125 YHPKLMWHEGFTVLGSPVDDAKKLWPNDYKRFCDVMEEYQNQMKGLGERLIRLIFKFLHI 184

Query: 183 SEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHDGL 242
           S++EM+K+LTST +S GKPYMALRLNSYPPCPDPSRV+GLAAHTDTSL TI+HQ R DGL
Sbjct: 185 SDKEMVKMLTSTCDSGGKPYMALRLNSYPPCPDPSRVVGLAAHTDTSLLTIIHQVRSDGL 244

Query: 243 QVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFYDP 302
           QVF DG GWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRV I ENKEHR+SL YFYDP
Sbjct: 245 QVFNDGAGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVTIQENKEHRYSLGYFYDP 304

Query: 303 PGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 350
           P  LYISPYCKPLSESPQIPLYRCVNV+EYFTIKA  +GKGLSAIKI
Sbjct: 305 PAGLYISPYCKPLSESPQIPLYRCVNVQEYFTIKASNKGKGLSAIKI 350

[Close]

BLAST of CmUC08G154850 vs. NCBI nr
Match: XP_008445138.1 (PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo])

HSP 1 Score: 615.5 bits (1586), Expect = 2.8e-172
Identity = 295/351 (84.05%), Postives = 322/351 (91.74%), Query Frame = 0


Query: 1   MADQELPPPSQLVPLDFNSIQT-VPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASE 60
           MADQE+PPPSQLVPL+F S+QT VPESHLWPN  ELS A+KLD+R+SLP+ID ++D ASE
Sbjct: 1   MADQEIPPPSQLVPLNFASVQTHVPESHLWPNVKELSAAVKLDKRVSLPLIDLVNDDASE 60

Query: 61  LLGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRT-DSLTATGYGMA 120
           LLGRACE+WGMFQLINHGV +TLI+EAEEETRRLF+LPA QKMKTLR+  SLTATGYGMA
Sbjct: 61  LLGRACEEWGMFQLINHGVSQTLIEEAEEETRRLFTLPAAQKMKTLRSPGSLTATGYGMA 120

Query: 121 GISKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFK 180
           GISKYH KLMWHEGFT+ GSP+++AKKLWPNDYKRFCDVMEEYQ QMKGLGERIIRLI K
Sbjct: 121 GISKYHPKLMWHEGFTIIGSPINEAKKLWPNDYKRFCDVMEEYQRQMKGLGERIIRLILK 180

Query: 181 FLDISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQAR 240
           FL ISEEEMMKLLTSTDES GKPYMALRLNSYPPCPDP +VMGLAAHTDTSL TI+HQ R
Sbjct: 181 FLGISEEEMMKLLTSTDESIGKPYMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVR 240

Query: 241 HDGLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAY 300
            +GLQ+FKDGTGWVPLSPMSGTL+VNVGD+LHILSNGRF SILHRVMI ENKEHRFSLAY
Sbjct: 241 SNGLQIFKDGTGWVPLSPMSGTLVVNVGDLLHILSNGRFTSILHRVMIQENKEHRFSLAY 300

Query: 301 FYDPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 350
           FYDPPGD+YISPYCKPLS++PQ PLYR VNVKEYF IKAKK G GL AIKI
Sbjct: 301 FYDPPGDIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKMGSGLPAIKI 351

[Close]

BLAST of CmUC08G154850 vs. NCBI nr
Match: XP_023002882.1 (gibberellin 3-beta-dioxygenase 1-like [Cucurbita maxima])

HSP 1 Score: 539.3 bits (1388), Expect = 2.5e-149
Identity = 263/349 (75.36%), Postives = 292/349 (83.67%), Query Frame = 0


Query: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60
           MADQE       +PLDFNS+QTVPESHLWP+S ELS  I LDE+ISLP ID LDDGASEL
Sbjct: 1   MADQE------FLPLDFNSVQTVPESHLWPDSEELSSVIDLDEKISLPFIDLLDDGASEL 60

Query: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGI 120
           LGRACEQWGMFQL NHG+ KTLI E EEE RRLF+LPA++KMK LR+     TGYG A I
Sbjct: 61  LGRACEQWGMFQLTNHGISKTLIAEMEEEARRLFTLPAKEKMKALRSAD-RVTGYGTAVI 120

Query: 121 SKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFL 180
           SKYH K MWHEGFT+ GSP +DAKKLWP+D KRFCDVMEEYQSQMKGL +R+IRLIFKFL
Sbjct: 121 SKYHPKCMWHEGFTILGSPANDAKKLWPSDSKRFCDVMEEYQSQMKGLSDRLIRLIFKFL 180

Query: 181 DISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHD 240
           DISEEE MKLL+ST +S GK Y AL+LNSYPPCPDP +VMGLAAHTDTSLFTIVHQ  +D
Sbjct: 181 DISEEETMKLLSSTGDS-GKSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQVCND 240

Query: 241 GLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFY 300
           GLQ+FKD   WVPLSPM G+ +VNVGD+LHI+SNGRFPSILHRV I   K HRFSLAYFY
Sbjct: 241 GLQIFKDRASWVPLSPMRGSFVVNVGDLLHIMSNGRFPSILHRVTIQGKKVHRFSLAYFY 300

Query: 301 DPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 350
            PP  LYISP+CKPLSESPQ PLYRCV+V+EYFT+KAK  G+GLS IKI
Sbjct: 301 YPPPYLYISPHCKPLSESPQAPLYRCVSVEEYFTMKAKNNGEGLSTIKI 341

[Close]

BLAST of CmUC08G154850 vs. ExPASy Swiss-Prot
Match: O24648 (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 2.6e-80
Identity = 146/338 (43.20%), Postives = 221/338 (65.38%), Query Frame = 0


Query: 16  DFNSIQTVPESHLW----PNSIELSPAIKLDERISLPIIDFLDDGASELLGRACEQWGMF 75
           DFNS+Q +PES+ W     +++  S  I  +   ++P+ID  D  AS+L+G AC+ WG++
Sbjct: 21  DFNSLQELPESYNWTHLDDHTLIDSNNIMKESTTTVPVIDLNDPNASKLIGLACKTWGVY 80

Query: 76  QLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGISKYHQKLMWHE 135
           Q++NHG+P +L+++ +   + LFSLP+ QK K  R+     +GYG+A IS +  KLMW+E
Sbjct: 81  QVMNHGIPLSLLEDIQWLGQTLFSLPSHQKHKATRSPD-GVSGYGIARISSFFPKLMWYE 140

Query: 136 GFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFLDISEEEMMKLL 195
           GFT+ GSP+D  ++LWP DY RFCD++ +Y   MK L   ++ L+   L I++E++    
Sbjct: 141 GFTIVGSPLDHFRELWPQDYTRFCDIVVQYDETMKKLAGTLMCLMLDSLGITKEDIK--W 200

Query: 196 TSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHDGLQVFKDGTGW 255
             +     K   AL+LNSYP CPDP   MGLA HTD++  TI+ Q    GLQV ++G+GW
Sbjct: 201 AGSKAQFEKACAALQLNSYPSCPDPDHAMGLAPHTDSTFLTILSQNDISGLQVNREGSGW 260

Query: 256 VPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFYDPPGDLYISPY 315
           + + P+ G L+VNVGD+ HILSNG +PS+LHRV+++  ++ RFS+AY Y PP ++ I P+
Sbjct: 261 ITVPPLQGGLVVNVGDLFHILSNGLYPSVLHRVLVNRTRQ-RFSVAYLYGPPSNVEICPH 320

Query: 316 CKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 350
            K +  + + PLYR V   EY   KAK   K LS++++
Sbjct: 321 AKLIGPT-KPPLYRSVTWNEYLGTKAKHFNKALSSVRL 353

[Close]

BLAST of CmUC08G154850 vs. ExPASy Swiss-Prot
Match: Q39103 (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 279.3 bits (713), Expect = 6.1e-74
Identity = 145/346 (41.91%), Postives = 214/346 (61.85%), Query Frame = 0


Query: 9   PSQLVPLDFNSIQTVPESHLWPNSIEL------SPAIKLDERISLPIIDFLDDGASELLG 68
           P   +P DF S++ +P+S+ W    +L      SP    +   ++P+ID     A+  +G
Sbjct: 17  PHSHIP-DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGE---NIPLIDLDHPDATNQIG 76

Query: 69  RACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGISK 128
            AC  WG FQ+ NHGVP  L+ + E  T  LF LP ++K+K+ R+++   +GYG+A I+ 
Sbjct: 77  HACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSET-GVSGYGVARIAS 136

Query: 129 YHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFLDI 188
           +  K MW EGFT++GSP++D +KLWP  +  +CD++EEY+  MK L  +++ L    L +
Sbjct: 137 FFNKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWLALNSLGV 196

Query: 189 SEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHDGL 248
           SEE++     S+D +  +   AL+LN YP CP+P R MGLAAHTD++L TI++Q    GL
Sbjct: 197 SEEDIEWASLSSDLNWAQ--AALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNTAGL 256

Query: 249 QVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFYDP 308
           QVF+D  GWV + P  G+L+VNVGD+ HILSNG F S+LHR  +++ +  R S+A+ + P
Sbjct: 257 QVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQTRA-RLSVAFLWGP 316

Query: 309 PGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIK 349
             D+ ISP  K L    + PLY+ V  KEY   KA    K LS I+
Sbjct: 317 QSDIKISPVPK-LVSPVESPLYQSVTWKEYLRTKATHFNKALSMIR 353

[Close]

BLAST of CmUC08G154850 vs. ExPASy Swiss-Prot
Match: Q9ZT84 (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 SV=2)

HSP 1 Score: 278.9 bits (712), Expect = 8.0e-74
Identity = 143/336 (42.56%), Postives = 210/336 (62.50%), Query Frame = 0


Query: 14  PLDFNSIQTVPESHLW-PNSIELSPAIKLDERISLPIIDFLDDGASELLGRACEQWGMFQ 73
           P DF S+   P+S+ W P    L  A   DE  +LP+ID  D   + L+G AC  WG FQ
Sbjct: 21  PPDFKSL---PDSYTWTPKDDLLFSASASDE--TLPLIDLSDIHVATLVGHACTTWGAFQ 80

Query: 74  LINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGISKYHQKLMWHEG 133
           + NHGVP  L+D+ E  T  LF LP ++K+K  R+++   +GYG+A I+ +  K MW EG
Sbjct: 81  ITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSEN-GVSGYGVARIASFFNKKMWSEG 140

Query: 134 FTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFLDISEEEMMKLLT 193
           FT+ GSP+ D +KLWP+ + ++C+++EEY+  M+ L  +++      L + E+++     
Sbjct: 141 FTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFALGSLGVEEKDIQWAGP 200

Query: 194 STDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHDGLQVFKDGTGWV 253
           ++D    +    ++LN YP CP+P R MGLAAHTD++L TI++Q    GLQVF+D  GWV
Sbjct: 201 NSDFQGTQ--AVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGWV 260

Query: 254 PLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFYDPPGDLYISPYC 313
              P+ G+L+VNVGD+LHIL+NG FPS+LHR  ++  +  RFS+AY + PP D+ ISP  
Sbjct: 261 TAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHVRS-RFSMAYLWGPPSDIMISPLP 320

Query: 314 KPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIK 349
           K L +  Q PLY  +  K+Y   KA    + LS I+
Sbjct: 321 K-LVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346

[Close]

BLAST of CmUC08G154850 vs. ExPASy Swiss-Prot
Match: Q9C971 (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 2.8e-66
Identity = 130/344 (37.79%), Postives = 207/344 (60.17%), Query Frame = 0


Query: 16  DFNSIQTVPESHLW-----PNSIELSPAIKLDE-RISLPIIDFLDDGASELLGRACEQWG 75
           +  S+QT+PES  W      + +  S A+  D    S+P+ID  +   + L+G A + WG
Sbjct: 12  NLGSLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWG 71

Query: 76  MFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGISKYHQKLMW 135
            FQ+ NHG+ + L+D+ E  ++ LF +P+ +K++   +D    +GYG   IS + +K MW
Sbjct: 72  AFQIANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDK-GVSGYGEPRISPFFEKKMW 131

Query: 136 HEGFTMS-GSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFLDISEE--E 195
            EGFT++  S  +    LWP+D+ ++C +++EY  +M+ L  R++  I   L ++ E  E
Sbjct: 132 SEGFTIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIE 191

Query: 196 MMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHDGLQVFK 255
               L  +    G+   A+RLN YP CP+P R MGLAAHTD+++ TI+HQ+   GLQVF+
Sbjct: 192 WAHKLEKSGSKVGRG--AIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFR 251

Query: 256 DGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFY-DPPGD 315
           + +GWV + P  G L+VN+GD+ HILSNG+ PS++HR  ++  +  R S+AY +  P GD
Sbjct: 252 EESGWVTVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTRS-RISIAYLWGGPAGD 311

Query: 316 LYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 350
           + I+P  K L+   +  LYR +  KEY  IK +   K + AI++
Sbjct: 312 VQIAPISK-LTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRV 350

[Close]

BLAST of CmUC08G154850 vs. ExPASy Swiss-Prot
Match: Q9SVS8 (Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.5e-64
Identity = 132/345 (38.26%), Postives = 204/345 (59.13%), Query Frame = 0


Query: 11  QLVPLDFNSIQTVPESHLW--PNSIELSPAIKLDERISLPIIDFLDDGASELLGRACEQW 70
           +++PLDF + +T+P+SH+W  P     S  I        P+I   +     LL +ACE+W
Sbjct: 17  RIIPLDFTNTKTLPDSHVWSKPEPETTSGPI--------PVISLSNPEEHGLLRQACEEW 76

Query: 71  GMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGISKYHQKLM 130
           G+F + +HGV  +L+   + + +RLFSLP  +K+  +R+    +TGYG+  IS ++ KLM
Sbjct: 77  GVFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPD-ESTGYGVVRISMFYDKLM 136

Query: 131 WHEGFTMSGSPV-DDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFLDISEEEM 190
           W EGF++ GS +   A  LWP+D+  FC+VMEEYQ  M  L  R+I ++   L ++ E++
Sbjct: 137 WSEGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISMLMGSLGLTHEDL 196

Query: 191 MKLL-TSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHDGLQV-- 250
             L+   T          L+LNSYP CPDP   MGLA HTD+SL TI++Q    GL++  
Sbjct: 197 GWLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIES 256

Query: 251 -FKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFYDPP 310
             ++G+ W+ + P+ G+L+V +GD+ HI+SNG+F S +HR ++++   HR S AYF  PP
Sbjct: 257 PQEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVNKT-HHRVSAAYFAGPP 316

Query: 311 GDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIK 349
            +L I P     S+    P+YR +  +EY   KA    K L+  +
Sbjct: 317 KNLQIGPL---TSDKNHPPIYRRLIWEEYLAAKATHFNKALTLFR 348

[Close]

BLAST of CmUC08G154850 vs. ExPASy TrEMBL
Match: Q9LM06 (Putative gibberellin 3 beta hydroxylase OS=Citrullus lanatus OX=3654 GN=WGA3H PE=2 SV=1)

HSP 1 Score: 723.0 bits (1865), Expect = 5.9e-205
Identity = 349/349 (100.00%), Postives = 349/349 (100.00%), Query Frame = 0


Query: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60
           MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL
Sbjct: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60

Query: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGI 120
           LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGI
Sbjct: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGI 120

Query: 121 SKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFL 180
           SKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFL
Sbjct: 121 SKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFL 180

Query: 181 DISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHD 240
           DISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHD
Sbjct: 181 DISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHD 240

Query: 241 GLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFY 300
           GLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFY
Sbjct: 241 GLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFY 300

Query: 301 DPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 350
           DPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI
Sbjct: 301 DPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 349

[Close]

BLAST of CmUC08G154850 vs. ExPASy TrEMBL
Match: S0BE47 (GA 3-oxidase OS=Cucumis sativus OX=3659 GN=GA3ox4 PE=2 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 3.4e-176
Identity = 296/350 (84.57%), Postives = 325/350 (92.86%), Query Frame = 0


Query: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60
           MADQE+PPPSQLVPLDF S+QTVPESHLWPN+ ELS AIKLD+R+SLP+ID + DGASEL
Sbjct: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60

Query: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRT-DSLTATGYGMAG 120
           LGRACE+WGMFQLINHG+ KTLI+EAEEETRRLF+LPA QKMKTLR+  SLTATGYGMAG
Sbjct: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120

Query: 121 ISKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKF 180
           ISKYH KLMWHEGFT+ GSP+++AKKLWPNDYKRFCDVMEEYQ +MKGLGERIIRLI KF
Sbjct: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180

Query: 181 LDISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARH 240
           L ISEEEMMKLLTSTDES GKP+MALRLNSYPPCPDP +VMGLAAHTDTSL TI+HQ  +
Sbjct: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240

Query: 241 DGLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYF 300
           +GLQ+FKDGTGWVPLSPMSGTLIVN+GD+LHILSNGRFPSILHRVMI ENKEHRFSLAYF
Sbjct: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300

Query: 301 YDPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 350
           YDPPG++YISPYCKPLS++PQ PLYR VNVKEYF IKAKK GKGL AIKI
Sbjct: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 350

[Close]

BLAST of CmUC08G154850 vs. ExPASy TrEMBL
Match: A0A1S3BBH9 (gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103488263 PE=3 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.3e-172
Identity = 295/351 (84.05%), Postives = 322/351 (91.74%), Query Frame = 0


Query: 1   MADQELPPPSQLVPLDFNSIQT-VPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASE 60
           MADQE+PPPSQLVPL+F S+QT VPESHLWPN  ELS A+KLD+R+SLP+ID ++D ASE
Sbjct: 1   MADQEIPPPSQLVPLNFASVQTHVPESHLWPNVKELSAAVKLDKRVSLPLIDLVNDDASE 60

Query: 61  LLGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRT-DSLTATGYGMA 120
           LLGRACE+WGMFQLINHGV +TLI+EAEEETRRLF+LPA QKMKTLR+  SLTATGYGMA
Sbjct: 61  LLGRACEEWGMFQLINHGVSQTLIEEAEEETRRLFTLPAAQKMKTLRSPGSLTATGYGMA 120

Query: 121 GISKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFK 180
           GISKYH KLMWHEGFT+ GSP+++AKKLWPNDYKRFCDVMEEYQ QMKGLGERIIRLI K
Sbjct: 121 GISKYHPKLMWHEGFTIIGSPINEAKKLWPNDYKRFCDVMEEYQRQMKGLGERIIRLILK 180

Query: 181 FLDISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQAR 240
           FL ISEEEMMKLLTSTDES GKPYMALRLNSYPPCPDP +VMGLAAHTDTSL TI+HQ R
Sbjct: 181 FLGISEEEMMKLLTSTDESIGKPYMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVR 240

Query: 241 HDGLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAY 300
            +GLQ+FKDGTGWVPLSPMSGTL+VNVGD+LHILSNGRF SILHRVMI ENKEHRFSLAY
Sbjct: 241 SNGLQIFKDGTGWVPLSPMSGTLVVNVGDLLHILSNGRFTSILHRVMIQENKEHRFSLAY 300

Query: 301 FYDPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 350
           FYDPPGD+YISPYCKPLS++PQ PLYR VNVKEYF IKAKK G GL AIKI
Sbjct: 301 FYDPPGDIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKMGSGLPAIKI 351

[Close]

BLAST of CmUC08G154850 vs. ExPASy TrEMBL
Match: A0A6J1KKU0 (gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111496619 PE=3 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 1.2e-149
Identity = 263/349 (75.36%), Postives = 292/349 (83.67%), Query Frame = 0


Query: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60
           MADQE       +PLDFNS+QTVPESHLWP+S ELS  I LDE+ISLP ID LDDGASEL
Sbjct: 1   MADQE------FLPLDFNSVQTVPESHLWPDSEELSSVIDLDEKISLPFIDLLDDGASEL 60

Query: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGI 120
           LGRACEQWGMFQL NHG+ KTLI E EEE RRLF+LPA++KMK LR+     TGYG A I
Sbjct: 61  LGRACEQWGMFQLTNHGISKTLIAEMEEEARRLFTLPAKEKMKALRSAD-RVTGYGTAVI 120

Query: 121 SKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFL 180
           SKYH K MWHEGFT+ GSP +DAKKLWP+D KRFCDVMEEYQSQMKGL +R+IRLIFKFL
Sbjct: 121 SKYHPKCMWHEGFTILGSPANDAKKLWPSDSKRFCDVMEEYQSQMKGLSDRLIRLIFKFL 180

Query: 181 DISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHD 240
           DISEEE MKLL+ST +S GK Y AL+LNSYPPCPDP +VMGLAAHTDTSLFTIVHQ  +D
Sbjct: 181 DISEEETMKLLSSTGDS-GKSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQVCND 240

Query: 241 GLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFY 300
           GLQ+FKD   WVPLSPM G+ +VNVGD+LHI+SNGRFPSILHRV I   K HRFSLAYFY
Sbjct: 241 GLQIFKDRASWVPLSPMRGSFVVNVGDLLHIMSNGRFPSILHRVTIQGKKVHRFSLAYFY 300

Query: 301 DPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 350
            PP  LYISP+CKPLSESPQ PLYRCV+V+EYFT+KAK  G+GLS IKI
Sbjct: 301 YPPPYLYISPHCKPLSESPQAPLYRCVSVEEYFTMKAKNNGEGLSTIKI 341

[Close]

BLAST of CmUC08G154850 vs. ExPASy TrEMBL
Match: A0A6J1GIY0 (gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111454608 PE=3 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 2.3e-148
Identity = 261/349 (74.79%), Postives = 291/349 (83.38%), Query Frame = 0


Query: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60
           MADQE       +PLDFNS+QTVPESHLWP+S ELS  I LDE+ISLP ID LDDGASEL
Sbjct: 1   MADQE------FLPLDFNSVQTVPESHLWPDSDELSSVIDLDEKISLPSIDLLDDGASEL 60

Query: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGI 120
           LGRACEQWGMFQL NHG+ KTLI E EEE RRLF+LP ++KMKTLR+     TGYG A I
Sbjct: 61  LGRACEQWGMFQLTNHGISKTLIAETEEEARRLFTLPVKEKMKTLRSAD-RVTGYGTAVI 120

Query: 121 SKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFL 180
           SKYH K MWHEGFT+ GSP DDAKKLWP+D KRFCDVMEEYQS+MK L +R+I+LIFKFL
Sbjct: 121 SKYHPKCMWHEGFTILGSPADDAKKLWPSDSKRFCDVMEEYQSEMKRLSDRLIKLIFKFL 180

Query: 181 DISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHD 240
           DIS EE MKLL+S D+S GK Y AL+LNSYPPCPDP +VMGLAAHTDTSLFTIVHQA +D
Sbjct: 181 DISVEETMKLLSSMDDS-GKSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACND 240

Query: 241 GLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFY 300
           GLQ+FKD  GWVPLSPM G+ +VNVGD+LHI+SNGRF SILHRV I   K HRFSLAYFY
Sbjct: 241 GLQIFKDKAGWVPLSPMRGSFVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFY 300

Query: 301 DPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 350
            PP  LYISP+CKPLSESPQ PLYRCV+VKEYFT+KAK  G+GLS +KI
Sbjct: 301 YPPPYLYISPHCKPLSESPQTPLYRCVSVKEYFTMKAKNNGEGLSTVKI 341

[Close]

BLAST of CmUC08G154850 vs. TAIR 10
Match: AT1G15550.1 (gibberellin 3-oxidase 1 )

HSP 1 Score: 279.3 bits (713), Expect = 4.4e-75
Identity = 145/346 (41.91%), Postives = 214/346 (61.85%), Query Frame = 0


Query: 9   PSQLVPLDFNSIQTVPESHLWPNSIEL------SPAIKLDERISLPIIDFLDDGASELLG 68
           P   +P DF S++ +P+S+ W    +L      SP    +   ++P+ID     A+  +G
Sbjct: 17  PHSHIP-DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGE---NIPLIDLDHPDATNQIG 76

Query: 69  RACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGISK 128
            AC  WG FQ+ NHGVP  L+ + E  T  LF LP ++K+K+ R+++   +GYG+A I+ 
Sbjct: 77  HACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSET-GVSGYGVARIAS 136

Query: 129 YHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFLDI 188
           +  K MW EGFT++GSP++D +KLWP  +  +CD++EEY+  MK L  +++ L    L +
Sbjct: 137 FFNKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWLALNSLGV 196

Query: 189 SEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHDGL 248
           SEE++     S+D +  +   AL+LN YP CP+P R MGLAAHTD++L TI++Q    GL
Sbjct: 197 SEEDIEWASLSSDLNWAQ--AALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNTAGL 256

Query: 249 QVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFYDP 308
           QVF+D  GWV + P  G+L+VNVGD+ HILSNG F S+LHR  +++ +  R S+A+ + P
Sbjct: 257 QVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQTRA-RLSVAFLWGP 316

Query: 309 PGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIK 349
             D+ ISP  K L    + PLY+ V  KEY   KA    K LS I+
Sbjct: 317 QSDIKISPVPK-LVSPVESPLYQSVTWKEYLRTKATHFNKALSMIR 353

[Close]

BLAST of CmUC08G154850 vs. TAIR 10
Match: AT1G80340.1 (gibberellin 3-oxidase 2 )

HSP 1 Score: 278.9 bits (712), Expect = 5.7e-75
Identity = 143/336 (42.56%), Postives = 210/336 (62.50%), Query Frame = 0


Query: 14  PLDFNSIQTVPESHLW-PNSIELSPAIKLDERISLPIIDFLDDGASELLGRACEQWGMFQ 73
           P DF S+   P+S+ W P    L  A   DE  +LP+ID  D   + L+G AC  WG FQ
Sbjct: 21  PPDFKSL---PDSYTWTPKDDLLFSASASDE--TLPLIDLSDIHVATLVGHACTTWGAFQ 80

Query: 74  LINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGISKYHQKLMWHEG 133
           + NHGVP  L+D+ E  T  LF LP ++K+K  R+++   +GYG+A I+ +  K MW EG
Sbjct: 81  ITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSEN-GVSGYGVARIASFFNKKMWSEG 140

Query: 134 FTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFLDISEEEMMKLLT 193
           FT+ GSP+ D +KLWP+ + ++C+++EEY+  M+ L  +++      L + E+++     
Sbjct: 141 FTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFALGSLGVEEKDIQWAGP 200

Query: 194 STDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHDGLQVFKDGTGWV 253
           ++D    +    ++LN YP CP+P R MGLAAHTD++L TI++Q    GLQVF+D  GWV
Sbjct: 201 NSDFQGTQ--AVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGWV 260

Query: 254 PLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFYDPPGDLYISPYC 313
              P+ G+L+VNVGD+LHIL+NG FPS+LHR  ++  +  RFS+AY + PP D+ ISP  
Sbjct: 261 TAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHVRS-RFSMAYLWGPPSDIMISPLP 320

Query: 314 KPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIK 349
           K L +  Q PLY  +  K+Y   KA    + LS I+
Sbjct: 321 K-LVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346

[Close]

BLAST of CmUC08G154850 vs. TAIR 10
Match: AT1G80330.1 (gibberellin 3-oxidase 4 )

HSP 1 Score: 253.8 bits (647), Expect = 2.0e-67
Identity = 130/344 (37.79%), Postives = 207/344 (60.17%), Query Frame = 0


Query: 16  DFNSIQTVPESHLW-----PNSIELSPAIKLDE-RISLPIIDFLDDGASELLGRACEQWG 75
           +  S+QT+PES  W      + +  S A+  D    S+P+ID  +   + L+G A + WG
Sbjct: 12  NLGSLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWG 71

Query: 76  MFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGISKYHQKLMW 135
            FQ+ NHG+ + L+D+ E  ++ LF +P+ +K++   +D    +GYG   IS + +K MW
Sbjct: 72  AFQIANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDK-GVSGYGEPRISPFFEKKMW 131

Query: 136 HEGFTMS-GSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFLDISEE--E 195
            EGFT++  S  +    LWP+D+ ++C +++EY  +M+ L  R++  I   L ++ E  E
Sbjct: 132 SEGFTIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIE 191

Query: 196 MMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHDGLQVFK 255
               L  +    G+   A+RLN YP CP+P R MGLAAHTD+++ TI+HQ+   GLQVF+
Sbjct: 192 WAHKLEKSGSKVGRG--AIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFR 251

Query: 256 DGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFY-DPPGD 315
           + +GWV + P  G L+VN+GD+ HILSNG+ PS++HR  ++  +  R S+AY +  P GD
Sbjct: 252 EESGWVTVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTRS-RISIAYLWGGPAGD 311

Query: 316 LYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 350
           + I+P  K L+   +  LYR +  KEY  IK +   K + AI++
Sbjct: 312 VQIAPISK-LTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRV 350

[Close]

BLAST of CmUC08G154850 vs. TAIR 10
Match: AT4G21690.1 (gibberellin 3-oxidase 3 )

HSP 1 Score: 248.1 bits (632), Expect = 1.1e-65
Identity = 132/345 (38.26%), Postives = 204/345 (59.13%), Query Frame = 0


Query: 11  QLVPLDFNSIQTVPESHLW--PNSIELSPAIKLDERISLPIIDFLDDGASELLGRACEQW 70
           +++PLDF + +T+P+SH+W  P     S  I        P+I   +     LL +ACE+W
Sbjct: 17  RIIPLDFTNTKTLPDSHVWSKPEPETTSGPI--------PVISLSNPEEHGLLRQACEEW 76

Query: 71  GMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGISKYHQKLM 130
           G+F + +HGV  +L+   + + +RLFSLP  +K+  +R+    +TGYG+  IS ++ KLM
Sbjct: 77  GVFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPD-ESTGYGVVRISMFYDKLM 136

Query: 131 WHEGFTMSGSPV-DDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFLDISEEEM 190
           W EGF++ GS +   A  LWP+D+  FC+VMEEYQ  M  L  R+I ++   L ++ E++
Sbjct: 137 WSEGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISMLMGSLGLTHEDL 196

Query: 191 MKLL-TSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHDGLQV-- 250
             L+   T          L+LNSYP CPDP   MGLA HTD+SL TI++Q    GL++  
Sbjct: 197 GWLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIES 256

Query: 251 -FKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFYDPP 310
             ++G+ W+ + P+ G+L+V +GD+ HI+SNG+F S +HR ++++   HR S AYF  PP
Sbjct: 257 PQEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVNKT-HHRVSAAYFAGPP 316

Query: 311 GDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIK 349
            +L I P     S+    P+YR +  +EY   KA    K L+  +
Sbjct: 317 KNLQIGPL---TSDKNHPPIYRRLIWEEYLAAKATHFNKALTLFR 348

[Close]

BLAST of CmUC08G154850 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 184.1 bits (466), Expect = 1.9e-46
Identity = 104/310 (33.55%), Postives = 164/310 (52.90%), Query Frame = 0


Query: 47  LPIIDFL-------DDGASEL--LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLP 106
           +P+ID         DD   E+  L +ACE WG FQ+INHG+   ++++ EE     F +P
Sbjct: 55  IPVIDLSKLSKPDNDDFFFEILKLSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDMP 114

Query: 107 ARQKMKTLRTDSLTATGYGMAGISKYHQKLMWHEGFTMSGSPVDDAK-KLWPNDYKRFCD 166
             +K K    +  T  GYG A I    QKL W   F +   P      KLWP+   RF +
Sbjct: 115 LEEK-KKYPMEPGTVQGYGQAFIFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARFSE 174

Query: 167 VMEEYQSQMKGLGERIIRLIFKFLDISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDP 226
            +E Y  +++ L +R+++ I   L + EE         +E  G+   A+R+N YPPC  P
Sbjct: 175 SLEGYSKEIRELCKRLLKYIAISLGLKEERF-------EEMFGEAVQAVRMNYYPPCSSP 234

Query: 227 SRVMGLAAHTDTSLFTIVHQARHD--GLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSN 286
             V+GL+ H+D S  T++ Q+++   GLQ+ KD T WVP+ P+   L++N+GD + +LSN
Sbjct: 235 DLVLGLSPHSDGSALTVLQQSKNSCVGLQILKDNT-WVPVKPLPNALVINIGDTIEVLSN 294

Query: 287 GRFPSILHRVMIHENKEHRFSLAYFYDPPGDLYISPYCKPLSESPQIPLYRCVNVKE--Y 343
           G++ S+ HR + +  KE R ++  FY P  ++ I P  + + +      YR  N  +  Y
Sbjct: 295 GKYKSVEHRAVTNREKE-RLTIVTFYAPNYEVEIEPMSELVDDETNPCKYRSYNHGDYSY 354

The following BLAST results are available for this feature:

BLAST of CmUC08G154850 vs. NCBI nr


Match Name	E-value	Identity	Description
AAF80661.1	1.2e-204	100.00	putative gibberellin 3 beta hydroxylase [Citrullus lanatus subsp. vulgaris]	[more]
NP_001275523.1	7.0e-176	84.57	gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus] >KAE8652189.1 hypothetic...	[more]
XP_038885582.1	1.6e-175	85.88	gibberellin 3-beta-dioxygenase 1-like [Benincasa hispida]	[more]
XP_008445138.1	2.8e-172	84.05	PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo]	[more]
XP_023002882.1	2.5e-149	75.36	gibberellin 3-beta-dioxygenase 1-like [Cucurbita maxima]	[more]

BLAST of CmUC08G154850 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O24648	2.6e-80	43.20	Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1	[more]
Q39103	6.1e-74	41.91	Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 ...	[more]
Q9ZT84	8.0e-74	42.56	Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 ...	[more]
Q9C971	2.8e-66	37.79	Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 ...	[more]
Q9SVS8	1.5e-64	38.26	Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 ...	[more]

BLAST of CmUC08G154850 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
Q9LM06	5.9e-205	100.00	Putative gibberellin 3 beta hydroxylase OS=Citrullus lanatus OX=3654 GN=WGA3H PE...	[more]
S0BE47	3.4e-176	84.57	GA 3-oxidase OS=Cucumis sativus OX=3659 GN=GA3ox4 PE=2 SV=1	[more]
A0A1S3BBH9	1.3e-172	84.05	gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103488263 PE...	[more]
A0A6J1KKU0	1.2e-149	75.36	gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC11149661...	[more]
A0A6J1GIY0	2.3e-148	74.79	gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111454...	[more]

BLAST of CmUC08G154850 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G15550.1	4.4e-75	41.91	gibberellin 3-oxidase 1	[more]
AT1G80340.1	5.7e-75	42.56	gibberellin 3-oxidase 2	[more]
AT1G80330.1	2.0e-67	37.79	gibberellin 3-oxidase 4	[more]
AT4G21690.1	1.1e-65	38.26	gibberellin 3-oxidase 3	[more]
AT3G21420.1	1.9e-46	33.55	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 61..78 score: 40.4 coord: 261..287 score: 31.99 coord: 225..246 score: 38.84
None	No IPR available	PANTHER	PTHR47991:SF77	GIBBERELLIN 3-BETA-DIOXYGENASE 1-LIKE	coord: 11..340
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 11..340
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 13..340
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 47..148 e-value: 1.4E-20 score: 74.2
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 204..302 e-value: 2.2E-27 score: 95.5
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 12..343 e-value: 6.9E-81 score: 274.1
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 200..302 score: 11.631144

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmUC08G154850.1	CmUC08G154850.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding