CmUC08G146240 (gene) Watermelon (USVL531) v1

Overview


Name	CmUC08G146240
Type	gene
Organism	Citrullus mucosospermus (Watermelon (USVL531) v1)
Description	rRNA N-glycosidase
Location	CmU531Chr08: 12281169 .. 12282092 (+)
RNA-Seq Expression	CmUC08G146240
Synteny	CmUC08G146240

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGAAATCCATAGCAATTTTCTTTTTCATTGTTGCCATCTTTGTTGGCTATCCTACTGTGAAGGGCGATGTTAGCTTCCATTTAGGGACCGCCACTGAAAAATCTTACACTTCTTTCATCGGACAGCTGAGGAATGCTCTTCCAACTACAGGTAAAGTCTGCAATATTCCTGTACTGCCTTCCACTGCATCAGGTTTACAATGGTTCACATTCTTCAGTCTTACAAATTATAACGATGAAACCATCACGGTGGCTGTAAACGTAACAAATGTTTATATCGTGGCGTATCGTGCCGGTGCTGTATCCTACTTTTTTGAAGACACTTCTGCTGAAGCATGGAATCTCCTATTTACAGATACTCGAAAGGTAAAACTTCCATATTCAGGTAATTATGATAGACTTCAAAATGTTGTTGGAAAACAAAGGGATTTCATTGAGCTTGGATTCCCTGCTTTAAGCAGCGCCATTACGAACATGTTTTACTACGACTACAAGCCTACTGCAGCTGCACTTCTTGTACTCATTCAGTCCACTGCAGAAGCTGCCAGGTTTAAATATATTGAGCAACAAATTTCTCAATATATTAACCATAACTTTTTACCAGATTTAGCAATTATAAGTTTAGAAAACAATTGGGGTGCTCTCTCCAAACAAATCCAGATTGCAAATAGTAGAGGAAACGGACAATTCGAAAGCCCTATTGACCTCATAACCCCTAATGGCACACGAGTTAGTGTAACCAATACTTCTGCTGGAGTTGTAAAAGGCAATATCAAACTTTTACTATTCTACAATTTATTAGCCAATGTTGTAGCTGATGACACTGACATGATTACTTCAATGTTGCATCCTGGAGCTATTATGAGTCCAATAAATTCTGGCTACAAAAAGGAAACCAAATTGGTTCAGCTATCAATATGA

mRNA sequence

ATGATGAAATCCATAGCAATTTTCTTTTTCATTGTTGCCATCTTTGTTGGCTATCCTACTGTGAAGGGCGATGTTAGCTTCCATTTAGGGACCGCCACTGAAAAATCTTACACTTCTTTCATCGGACAGCTGAGGAATGCTCTTCCAACTACAGGTAAAGTCTGCAATATTCCTGTACTGCCTTCCACTGCATCAGGTTTACAATGGTTCACATTCTTCAGTCTTACAAATTATAACGATGAAACCATCACGGTGGCTGTAAACGTAACAAATGTTTATATCGTGGCGTATCGTGCCGGTGCTGTATCCTACTTTTTTGAAGACACTTCTGCTGAAGCATGGAATCTCCTATTTACAGATACTCGAAAGGTAAAACTTCCATATTCAGGTAATTATGATAGACTTCAAAATGTTGTTGGAAAACAAAGGGATTTCATTGAGCTTGGATTCCCTGCTTTAAGCAGCGCCATTACGAACATGTTTTACTACGACTACAAGCCTACTGCAGCTGCACTTCTTGTACTCATTCAGTCCACTGCAGAAGCTGCCAGGTTTAAATATATTGAGCAACAAATTTCTCAATATATTAACCATAACTTTTTACCAGATTTAGCAATTATAAGTTTAGAAAACAATTGGGGTGCTCTCTCCAAACAAATCCAGATTGCAAATAGTAGAGGAAACGGACAATTCGAAAGCCCTATTGACCTCATAACCCCTAATGGCACACGAGTTAGTGTAACCAATACTTCTGCTGGAGTTGTAAAAGGCAATATCAAACTTTTACTATTCTACAATTTATTAGCCAATGTTGTAGCTGATGACACTGACATGATTACTTCAATGTTGCATCCTGGAGCTATTATGAGTCCAATAAATTCTGGCTACAAAAAGGAAACCAAATTGGTTCAGCTATCAATATGA

Coding sequence (CDS)

ATGATGAAATCCATAGCAATTTTCTTTTTCATTGTTGCCATCTTTGTTGGCTATCCTACTGTGAAGGGCGATGTTAGCTTCCATTTAGGGACCGCCACTGAAAAATCTTACACTTCTTTCATCGGACAGCTGAGGAATGCTCTTCCAACTACAGGTAAAGTCTGCAATATTCCTGTACTGCCTTCCACTGCATCAGGTTTACAATGGTTCACATTCTTCAGTCTTACAAATTATAACGATGAAACCATCACGGTGGCTGTAAACGTAACAAATGTTTATATCGTGGCGTATCGTGCCGGTGCTGTATCCTACTTTTTTGAAGACACTTCTGCTGAAGCATGGAATCTCCTATTTACAGATACTCGAAAGGTAAAACTTCCATATTCAGGTAATTATGATAGACTTCAAAATGTTGTTGGAAAACAAAGGGATTTCATTGAGCTTGGATTCCCTGCTTTAAGCAGCGCCATTACGAACATGTTTTACTACGACTACAAGCCTACTGCAGCTGCACTTCTTGTACTCATTCAGTCCACTGCAGAAGCTGCCAGGTTTAAATATATTGAGCAACAAATTTCTCAATATATTAACCATAACTTTTTACCAGATTTAGCAATTATAAGTTTAGAAAACAATTGGGGTGCTCTCTCCAAACAAATCCAGATTGCAAATAGTAGAGGAAACGGACAATTCGAAAGCCCTATTGACCTCATAACCCCTAATGGCACACGAGTTAGTGTAACCAATACTTCTGCTGGAGTTGTAAAAGGCAATATCAAACTTTTACTATTCTACAATTTATTAGCCAATGTTGTAGCTGATGACACTGACATGATTACTTCAATGTTGCATCCTGGAGCTATTATGAGTCCAATAAATTCTGGCTACAAAAAGGAAACCAAATTGGTTCAGCTATCAATATGA

Protein sequence

MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVLPSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTDTRKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTAEAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITPNGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSMLHPGAIMSPINSGYKKETKLVQLSI

Homology

[Close]

BLAST of CmUC08G146240 vs. NCBI nr
Match: P56626.2 (RecName: Full=Type I ribosome-inactivating protein trichoanguina; Short=RIP; AltName: Full=Trichoanguin; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Trichosanthes anguina] >AAD02686.1 trichoanguin [Trichosanthes cucumerina])

HSP 1 Score: 404.1 bits (1037), Expect = 1.1e-108
Identity = 206/284 (72.54%), Postives = 239/284 (84.15%), Query Frame = 0


Query: 5   IAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVLPSTA 64
           +A+ FF +AI +G PT  GDVSF L TAT+KSY+SFI QLR+ALPT G VC IP+LPSTA
Sbjct: 1   MALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTA 60

Query: 65  SGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTDTRKV 124
           SG QWF FF+LTNYNDET+TVAVNVTNVYIVAYRA AVSYFFEDT AEA+ L+F  T+ V
Sbjct: 61  SGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTV 120

Query: 125 KLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTAEAAR 184
           KLPYSGNYD+LQ+VVGKQRD IELG PALSSAITNM YYDY+ TAAALLVLIQ TAEAAR
Sbjct: 121 KLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTAEAAR 180

Query: 185 FKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITPNGTR 244
           +KYIEQQ+S +I+ NF P+ A+ISLEN WGALSKQIQIAN  G+GQFE+P++L  P+GTR
Sbjct: 181 YKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTR 240

Query: 245 VSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSMLHPGAI 289
            SVTNTSAGVVKGNIKLLL+Y      V  + D+ T++LHPGA+
Sbjct: 241 FSVTNTSAGVVKGNIKLLLYYKA---SVGSEYDIPTTILHPGAM 281

[Close]

BLAST of CmUC08G146240 vs. NCBI nr
Match: XP_022156709.1 (type I ribosome-inactivating protein trichoanguina-like [Momordica charantia])

HSP 1 Score: 392.5 bits (1007), Expect = 3.3e-105
Identity = 207/289 (71.63%), Postives = 238/289 (82.35%), Query Frame = 0


Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVL 60
           MMKSIA+ FFI+ IF+G PT KGDVSF L TAT+ SYTSFI  LR+ALPT G VCNIPVL
Sbjct: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTD 120
           PSTASGLQWF FFSLTNYN +TITVAVNVTNVYI AY  GAVSYFFEDTSAEA+ LLF  
Sbjct: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFY-YDYKPTAAALLVLIQST 180
           T+KV LPYSGNYDRLQ++VGK RD IELG P LS+AITN+FY  +YK TA ALLVLIQST
Sbjct: 121 TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST 180

Query: 181 AEAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLIT 240
           AEAARFKYIEQ++S++++ NF P+ AIISLEN+WGALSKQIQIANS+G GQF+  ++LIT
Sbjct: 181 AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT 240

Query: 241 PNGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSMLHPGAI 289
            +G RVSVTNTS GVVK NIKLLL+Y     V   + + I ++L  GAI
Sbjct: 241 IDGKRVSVTNTSTGVVKSNIKLLLYY----KVNGANDNYIPTILPLGAI 285

[Close]

BLAST of CmUC08G146240 vs. NCBI nr
Match: XP_023005055.1 (type I ribosome-inactivating protein trichoanguina-like [Cucurbita maxima])

HSP 1 Score: 381.7 bits (979), Expect = 5.9e-102
Identity = 198/282 (70.21%), Postives = 236/282 (83.69%), Query Frame = 0


Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M K IAI  F+VAI +  PT+K D++F L TAT+ SY++FI QLRNALPTT  VCNIP+L
Sbjct: 1   MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTD 120
           PSTASGL WFTFF++TNYN +TITVAVNVTNVYIVAYRA A SYFFEDT AEA  LLF +
Sbjct: 61  PSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPN 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           T+KV LP++GNYD+LQ++VGK RD IELG PALS+A+TNM YY+ + TAAALLVLIQS+A
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITP 240
           EAARFKYIEQQ++Q+I+  F P+ A+ISLENNWGALSKQIQIANS G GQF+SP++LI+P
Sbjct: 181 EAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSM 283
           NGTR SVT+TSAGVV+ NIKLLL+Y L    VAD+ D  T M
Sbjct: 241 NGTRFSVTSTSAGVVQSNIKLLLYYKL---SVADENDSPTRM 279

[Close]

BLAST of CmUC08G146240 vs. NCBI nr
Match: KAG6592904.1 (Type I ribosome-inactivating protein trichoanguina, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025309.1 Type I ribosome-inactivating protein trichoanguina, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 378.6 bits (971), Expect = 5.0e-101
Identity = 193/282 (68.44%), Postives = 235/282 (83.33%), Query Frame = 0


Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M K IAI  F++AI +  PT+K D++F L TAT+ SY++FI QLRNALPT   VCNIP+L
Sbjct: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTD 120
           PSTA+GL WFTFF++TNYN +T+ VAVNV+NVYIVAYRA A SYFFEDT A+A  LLF  
Sbjct: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           T+KV LP++GNYD+LQ++VGK RD +ELG PALS+A+TNM YY+ +PTAAALLVLIQSTA
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITP 240
           EAARFKYIEQQ++QYI+  F P+ A+ISLENNWGALSKQIQIANS G+GQF+SP++LI+P
Sbjct: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSM 283
           NGTR  VT+TS+GVV+GNIKLLL+Y L    VADD D  T M
Sbjct: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKL---SVADDNDSPTRM 279

[Close]

BLAST of CmUC08G146240 vs. NCBI nr
Match: XP_022960460.1 (type I ribosome-inactivating protein trichoanguina-like [Cucurbita moschata])

HSP 1 Score: 370.5 bits (950), Expect = 1.4e-98
Identity = 193/282 (68.44%), Postives = 233/282 (82.62%), Query Frame = 0


Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M K IAI  F++AI +  PT+K D++  L TAT+ +Y++FI QLRNALPT   VCNIP+L
Sbjct: 1   MNKLIAITSFLLAISLISPTLKADIALDLSTATKATYSAFITQLRNALPTKATVCNIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTD 120
           PSTA+GL  FTFF++TNYN ET+ VAVNV+NVYIVAYRA A SYFFEDTSA+A  LLF +
Sbjct: 61  PSTATGLGSFTFFTITNYNYETVEVAVNVSNVYIVAYRANAKSYFFEDTSAQARQLLFQN 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           T+K+ LP++GNYDRLQ VVGK RD IELG PALS+A+TNM YY+ + TAAALLVLIQSTA
Sbjct: 121 TQKITLPFTGNYDRLQGVVGKYRDSIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSTA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITP 240
           EAARFKYIEQQ++QYI+  F P+ A+ISLENNWGALSKQIQIANS G+GQF+SP++LI+P
Sbjct: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSM 283
           NGTR  VT+TS+GVV+GNIKLLL+Y L    VADD D  T M
Sbjct: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKL---SVADDNDSPTRM 279

[Close]

BLAST of CmUC08G146240 vs. ExPASy Swiss-Prot
Match: P56626 (Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina OX=50544 GN=TCA PE=1 SV=2)

HSP 1 Score: 404.1 bits (1037), Expect = 1.5e-111
Identity = 206/284 (72.54%), Postives = 239/284 (84.15%), Query Frame = 0


Query: 5   IAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVLPSTA 64
           +A+ FF +AI +G PT  GDVSF L TAT+KSY+SFI QLR+ALPT G VC IP+LPSTA
Sbjct: 1   MALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTA 60

Query: 65  SGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTDTRKV 124
           SG QWF FF+LTNYNDET+TVAVNVTNVYIVAYRA AVSYFFEDT AEA+ L+F  T+ V
Sbjct: 61  SGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTV 120

Query: 125 KLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTAEAAR 184
           KLPYSGNYD+LQ+VVGKQRD IELG PALSSAITNM YYDY+ TAAALLVLIQ TAEAAR
Sbjct: 121 KLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTAEAAR 180

Query: 185 FKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITPNGTR 244
           +KYIEQQ+S +I+ NF P+ A+ISLEN WGALSKQIQIAN  G+GQFE+P++L  P+GTR
Sbjct: 181 YKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTR 240

Query: 245 VSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSMLHPGAI 289
            SVTNTSAGVVKGNIKLLL+Y      V  + D+ T++LHPGA+
Sbjct: 241 FSVTNTSAGVVKGNIKLLLYYKA---SVGSEYDIPTTILHPGAM 281

[Close]

BLAST of CmUC08G146240 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 292.0 bits (746), Expect = 8.0e-78
Identity = 157/282 (55.67%), Postives = 205/282 (72.70%), Query Frame = 0


Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M+K + + F I+AIF+G PT KGDV+F L TAT K+YT FI   R  LP + KV +IP+L
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTD 120
            ST S  + F   +LT+Y  ETI+VA++VTNVY+VAYR   VSYFF+++  EA+N+LF  
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           TRK+ LPY+GNY+ LQ    K R+ I+LG PALSSAIT +FYY+ +   +ALLVLIQ+TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITP 240
           EAARFKYIE+ +++Y+  NF P+LAIISLEN W ALSKQI +A ++G G+F +P+DLI P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSM 283
            G R  VTN  + VVKGNIKLLL  N  A+    D + IT+M
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLL--NSRASTA--DENFITTM 277

[Close]

BLAST of CmUC08G146240 vs. ExPASy Swiss-Prot
Match: P29339 (Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 1.1e-77
Identity = 157/282 (55.67%), Postives = 204/282 (72.34%), Query Frame = 0


Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M+K + + F I+AIF+G PT KGDV+F L TAT K+YT FI   R  LP + KV +IP+L
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTD 120
            ST S  + F    LT+Y  ETI+VA++VTNVY+VAYR   VSYFF+++  EA+N+LF  
Sbjct: 61  YSTISDSRRFILLDLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           TRK+ LPY+GNY+ LQ    K R+ I+LG PALSSAIT +FYY+ +   +ALLVLIQ+TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITP 240
           EAARFKYIE+ +++Y+  NF P+LAIISLEN W ALSKQI +A ++G G+F +P+DLI P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSM 283
            G R  VTN  + VVKGNIKLLL  N  A+    D + IT+M
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLL--NSRASTA--DENFITTM 277

[Close]

BLAST of CmUC08G146240 vs. ExPASy Swiss-Prot
Match: P33185 (Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3)

HSP 1 Score: 282.0 bits (720), Expect = 8.3e-75
Identity = 162/276 (58.70%), Postives = 193/276 (69.93%), Query Frame = 0


Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M+K + ++  I+ IF+  PTV+GDVSF L  AT  SY  FI  LR ALP   KV NIP+L
Sbjct: 1   MIKLLVLWLLILTIFLKSPTVEGDVSFRLSGATTTSYGVFIKNLREALPYERKVYNIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSA-EAWNLLFT 120
            S+ SG   +T   LTNY DETI+VAV+VTNVYI+ Y AG VSYFF + SA EA   +F 
Sbjct: 61  RSSISGSGRYTLLHLTNYADETISVAVDVTNVYIMGYLAGDVSYFFNEASATEAAKFVFK 120

Query: 121 DT-RKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQS 180
           D  +KV LPYSGNY+RLQ   GK R+ I LG PAL SAIT ++YY     A+ALLVLIQS
Sbjct: 121 DAKKKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLYYYTASSAASALLVLIQS 180

Query: 181 TAEAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLI 240
           TAE+AR+K+IEQQI + ++  FLP LA ISLENNW ALSKQIQIA S  NGQFESP+ LI
Sbjct: 181 TAESARYKFIEQQIGKRVDKTFLPSLATISLENNWSALSKQIQIA-STNNGQFESPVVLI 240

Query: 241 TPNGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVAD 275
             N  RVS+TN SA VV  NI LLL  N +A +  D
Sbjct: 241 DGNNQRVSITNASARVVTSNIALLLNRNNIAAIGED 275

[Close]

BLAST of CmUC08G146240 vs. ExPASy Swiss-Prot
Match: P09989 (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 277.3 bits (708), Expect = 2.1e-73
Identity = 157/283 (55.48%), Postives = 194/283 (68.55%), Query Frame = 0


Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M++ + +   I+ +F+  P V+GDVSF L  AT  SY  FI  LR ALP   K+ +IP+L
Sbjct: 1   MIRFLVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSA-EAWNLLFT 120
            S+  G Q +    LTNY DETI+VA++VTNVYI+ YRAG  SYFF + SA EA   +F 
Sbjct: 61  RSSLPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFK 120

Query: 121 DT-RKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQS 180
           D  RKV LPYSGNY+RLQ   GK R+ I LG PAL SAIT +FYY+    A+AL+VLIQS
Sbjct: 121 DAMRKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQS 180

Query: 181 TAEAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLI 240
           T+EAAR+K+IEQQI + ++  FLP LAIISLEN+W ALSKQIQIA S  NGQFESP+ LI
Sbjct: 181 TSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIA-STNNGQFESPVVLI 240

Query: 241 TPNGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITS 282
                RV++TN  AGVV  NI LLL  N +A  + DD  M  S
Sbjct: 241 NAQNQRVTITNVDAGVVTSNIALLLNRNNMA-AMDDDVPMTQS 281

[Close]

BLAST of CmUC08G146240 vs. ExPASy TrEMBL
Match: A0A6J1DUC7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023554 PE=3 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 1.6e-105
Identity = 207/289 (71.63%), Postives = 238/289 (82.35%), Query Frame = 0


Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVL 60
           MMKSIA+ FFI+ IF+G PT KGDVSF L TAT+ SYTSFI  LR+ALPT G VCNIPVL
Sbjct: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTD 120
           PSTASGLQWF FFSLTNYN +TITVAVNVTNVYI AY  GAVSYFFEDTSAEA+ LLF  
Sbjct: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFY-YDYKPTAAALLVLIQST 180
           T+KV LPYSGNYDRLQ++VGK RD IELG P LS+AITN+FY  +YK TA ALLVLIQST
Sbjct: 121 TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST 180

Query: 181 AEAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLIT 240
           AEAARFKYIEQ++S++++ NF P+ AIISLEN+WGALSKQIQIANS+G GQF+  ++LIT
Sbjct: 181 AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT 240

Query: 241 PNGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSMLHPGAI 289
            +G RVSVTNTS GVVK NIKLLL+Y     V   + + I ++L  GAI
Sbjct: 241 IDGKRVSVTNTSTGVVKSNIKLLLYY----KVNGANDNYIPTILPLGAI 285

[Close]

BLAST of CmUC08G146240 vs. ExPASy TrEMBL
Match: A0A6J1KWC4 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111498162 PE=3 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 2.9e-102
Identity = 198/282 (70.21%), Postives = 236/282 (83.69%), Query Frame = 0


Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M K IAI  F+VAI +  PT+K D++F L TAT+ SY++FI QLRNALPTT  VCNIP+L
Sbjct: 1   MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTD 120
           PSTASGL WFTFF++TNYN +TITVAVNVTNVYIVAYRA A SYFFEDT AEA  LLF +
Sbjct: 61  PSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPN 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           T+KV LP++GNYD+LQ++VGK RD IELG PALS+A+TNM YY+ + TAAALLVLIQS+A
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITP 240
           EAARFKYIEQQ++Q+I+  F P+ A+ISLENNWGALSKQIQIANS G GQF+SP++LI+P
Sbjct: 181 EAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSM 283
           NGTR SVT+TSAGVV+ NIKLLL+Y L    VAD+ D  T M
Sbjct: 241 NGTRFSVTSTSAGVVQSNIKLLLYYKL---SVADENDSPTRM 279

[Close]

BLAST of CmUC08G146240 vs. ExPASy TrEMBL
Match: A0A6J1H951 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111461188 PE=3 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 6.6e-99
Identity = 193/282 (68.44%), Postives = 233/282 (82.62%), Query Frame = 0


Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M K IAI  F++AI +  PT+K D++  L TAT+ +Y++FI QLRNALPT   VCNIP+L
Sbjct: 1   MNKLIAITSFLLAISLISPTLKADIALDLSTATKATYSAFITQLRNALPTKATVCNIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTD 120
           PSTA+GL  FTFF++TNYN ET+ VAVNV+NVYIVAYRA A SYFFEDTSA+A  LLF +
Sbjct: 61  PSTATGLGSFTFFTITNYNYETVEVAVNVSNVYIVAYRANAKSYFFEDTSAQARQLLFQN 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           T+K+ LP++GNYDRLQ VVGK RD IELG PALS+A+TNM YY+ + TAAALLVLIQSTA
Sbjct: 121 TQKITLPFTGNYDRLQGVVGKYRDSIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSTA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITP 240
           EAARFKYIEQQ++QYI+  F P+ A+ISLENNWGALSKQIQIANS G+GQF+SP++LI+P
Sbjct: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSM 283
           NGTR  VT+TS+GVV+GNIKLLL+Y L    VADD D  T M
Sbjct: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKL---SVADDNDSPTRM 279

[Close]

BLAST of CmUC08G146240 vs. ExPASy TrEMBL
Match: A0A6J1DR40 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023561 PE=3 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 2.0e-87
Identity = 171/268 (63.81%), Postives = 209/268 (77.99%), Query Frame = 0


Query: 2   MKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVLP 61
           M+ +     ++AI +G  + +GDVSF L  A +KSYT+FI QLR+A+PTT  VC IPVLP
Sbjct: 1   MRKLTTLSLLLAILLGPLSAEGDVSFQLSGANKKSYTNFIKQLRDAVPTTTTVCKIPVLP 60

Query: 62  STASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTDT 121
           STASG + FTF  LTNY D+T+ VA+N+TNVYIVAY    VSYFF+D S EA+ +LF  T
Sbjct: 61  STASGPKSFTFVHLTNYADQTVVVAINITNVYIVAYHIDHVSYFFDDASPEAYKVLFEGT 120

Query: 122 RKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTAE 181
            +  LPYS NYD+LQ++VGK+RD IELG PALSSAITN+ YY+YK TAAALLVLIQS AE
Sbjct: 121 DRKTLPYSSNYDKLQSIVGKKRDLIELGLPALSSAITNLVYYEYKSTAAALLVLIQSVAE 180

Query: 182 AARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITPN 241
           AAR+KYIEQ+IS +++  F PD AIISLENNWGALSKQIQIA ++GNGQFE P++LI PN
Sbjct: 181 AARYKYIEQEISTHVDDKFKPDQAIISLENNWGALSKQIQIA-AKGNGQFEKPVELINPN 240

Query: 242 GTRVSVTNTSAGVVKGNIKLLLFYNLLA 270
            T  SVTN S+ VV  NIKLLL++ L A
Sbjct: 241 NTPFSVTNVSSLVVTSNIKLLLYFKLAA 267

[Close]

BLAST of CmUC08G146240 vs. ExPASy TrEMBL
Match: Q0ZBB8 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=MAP30 PE=3 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 6.0e-76
Identity = 158/282 (56.03%), Postives = 205/282 (72.70%), Query Frame = 0


Query: 1   MMKSIAIFFFIVAIFVGYPTVKGDVSFHLGTATEKSYTSFIGQLRNALPTTGKVCNIPVL 60
           M+K + + F I+AIF+G PT KGDV+F L TAT K+YT FI   R  LP + KV +IP+L
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGLQWFTFFSLTNYNDETITVAVNVTNVYIVAYRAGAVSYFFEDTSAEAWNLLFTD 120
            ST S  + F   +LT+Y  ETI+VA++VTNVY+VAYR   VSYFF+++  EA+N+LF  
Sbjct: 61  YSTISDSRHFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TRKVKLPYSGNYDRLQNVVGKQRDFIELGFPALSSAITNMFYYDYKPTAAALLVLIQSTA 180
           TRK+ LPY+GNY+ LQ    K R+ I+LG PALSSAIT +FYY+ +   +ALLVLIQ+TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQISQYINHNFLPDLAIISLENNWGALSKQIQIANSRGNGQFESPIDLITP 240
           EAARFKYIE+ +++Y+  NF P+LAIISLEN W ALSKQI +A ++G G+F +P+DLI P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRVSVTNTSAGVVKGNIKLLLFYNLLANVVADDTDMITSM 283
            G R  VTN  + VVKGNIKLLL  N  A+    D D IT+M
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLL--NSRASTA--DEDFITTM 277

The following BLAST results are available for this feature:

BLAST of CmUC08G146240 vs. NCBI nr


Match Name	E-value	Identity	Description
P56626.2	1.1e-108	72.54	RecName: Full=Type I ribosome-inactivating protein trichoanguina; Short=RIP; Alt...	[more]
XP_022156709.1	3.3e-105	71.63	type I ribosome-inactivating protein trichoanguina-like [Momordica charantia]	[more]
XP_023005055.1	5.9e-102	70.21	type I ribosome-inactivating protein trichoanguina-like [Cucurbita maxima]	[more]
KAG6592904.1	5.0e-101	68.44	Type I ribosome-inactivating protein trichoanguina, partial [Cucurbita argyrospe...	[more]
XP_022960460.1	1.4e-98	68.44	type I ribosome-inactivating protein trichoanguina-like [Cucurbita moschata]	[more]

BLAST of CmUC08G146240 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P56626	1.5e-111	72.54	Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina OX=5...	[more]
P24817	8.0e-78	55.67	Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN...	[more]
P29339	1.1e-77	55.67	Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV...	[more]
P33185	8.3e-75	58.70	Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3	[more]
P09989	2.1e-73	55.48	Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=...	[more]

BLAST of CmUC08G146240 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DUC7	1.6e-105	71.63	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023554 PE=3 SV=1	[more]
A0A6J1KWC4	2.9e-102	70.21	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111498162 PE=3 SV=1	[more]
A0A6J1H951	6.6e-99	68.44	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111461188 PE=3 SV=1	[more]
A0A6J1DR40	2.0e-87	63.81	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023561 PE=3 SV=1	[more]
Q0ZBB8	6.0e-76	56.03	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=MAP30 PE=3 SV=1	[more]

BLAST of CmUC08G146240 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 143..157 score: 49.82 coord: 35..48 score: 44.17 coord: 82..97 score: 52.63 coord: 172..192 score: 66.29 coord: 204..221 score: 53.51
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 184..268 e-value: 1.8E-28 score: 100.2
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 33..221 e-value: 1.1E-32 score: 113.2
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 25..256
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 24..183 e-value: 5.6E-44 score: 151.6
None	No IPR available	PIRSR	PIRSR001924-1	PIRSR001924-1	coord: 6..218 e-value: 8.7E-5 score: 19.8
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 25..256
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 176..192
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 24..267

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmUC08G146240.1	CmUC08G146240.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity