CmUC08G146230 (gene) Watermelon (USVL531) v1

Overview


Name	CmUC08G146230
Type	gene
Organism	Citrullus mucosospermus (Watermelon (USVL531) v1)
Description	rRNA N-glycosidase
Location	CmU531Chr08: 12252758 .. 12253570 (-)
RNA-Seq Expression	CmUC08G146230
Synteny	CmUC08G146230

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGACTCTTAGGATTTTACTTCCTTCTTGCACTGTATGTTGGCGGCTGTGTTGTTGAAGCCGATGTAAGCTTCTCTATGTTGGGCGCCACAAGTGAAACCTACAAACAGTTCATAAAGAATCTGCGAAGTGCACTCACAGTTGGATCTCCGACAGTCTACAACATCCCCGTCTTGAAGAGCACAGCAACGGGGCCAGCAGGCTTTCTATTAGTTCACCTCACAAATTACAATGATGAATCCATCACGGTGGCAATAGACGTAGTGAACGTGTACGTGGTAGCATATGGAGCTGGAAACAATGCCTATTTCTTGAATGACGCTTCAACAGAAGCCAACAACCTTCTATTTAAAGGCCTCAAACATACAAGACTTCCTTACAAAGGCAATTACGATGGCCTTGAGACTGCTGCAGGAAAGATTTCAAGAGAGAAAATTGAACTTGGATTTTCTGAGATAAGCAGTGCCATTGGCAACATGTTCCACCACAATGCTGGTACTTCTGTCCCTAAAGCCTTCATTGTCATTATCGAAACTGTGTCTGAAGCTGCAAGATATAAATATATTGAGCAGAGAGTTTCTGAGAATGTGAAGACAAAGTTCTTGCCGGACCCTGCTTTCTTAAGCTTAGAGAATCGTTGGTCTGATTTATCCCAACAAGTTCAAATCGCACAGACGCGCGGAGGCCAGTTTGCTCGTGCTGTTGAGCTACGAACTGTTGGCAATAAACCAGTGCTCGTAACCAATGTTAGTTCGCCCATTGTCAAAGGAATTGCACTTCTGTTGTACTATAAACCCAGTGTTGCCGCCTAA

mRNA sequence

ATGAGACTCTTAGGATTTTACTTCCTTCTTGCACTGTATGTTGGCGGCTGTGTTGTTGAAGCCGATGTAAGCTTCTCTATGTTGGGCGCCACAAGTGAAACCTACAAACAGTTCATAAAGAATCTGCGAAGTGCACTCACAGTTGGATCTCCGACAGTCTACAACATCCCCGTCTTGAAGAGCACAGCAACGGGGCCAGCAGGCTTTCTATTAGTTCACCTCACAAATTACAATGATGAATCCATCACGGTGGCAATAGACGTAGTGAACGTGTACGTGGTAGCATATGGAGCTGGAAACAATGCCTATTTCTTGAATGACGCTTCAACAGAAGCCAACAACCTTCTATTTAAAGGCCTCAAACATACAAGACTTCCTTACAAAGGCAATTACGATGGCCTTGAGACTGCTGCAGGAAAGATTTCAAGAGAGAAAATTGAACTTGGATTTTCTGAGATAAGCAGTGCCATTGGCAACATGTTCCACCACAATGCTGGTACTTCTGTCCCTAAAGCCTTCATTGTCATTATCGAAACTGTGTCTGAAGCTGCAAGATATAAATATATTGAGCAGAGAGTTTCTGAGAATGTGAAGACAAAGTTCTTGCCGGACCCTGCTTTCTTAAGCTTAGAGAATCGTTGGTCTGATTTATCCCAACAAGTTCAAATCGCACAGACGCGCGGAGGCCAGTTTGCTCGTGCTGTTGAGCTACGAACTGTTGGCAATAAACCAGTGCTCGTAACCAATGTTAGTTCGCCCATTGTCAAAGGAATTGCACTTCTGTTGTACTATAAACCCAGTGTTGCCGCCTAA

Coding sequence (CDS)

ATGAGACTCTTAGGATTTTACTTCCTTCTTGCACTGTATGTTGGCGGCTGTGTTGTTGAAGCCGATGTAAGCTTCTCTATGTTGGGCGCCACAAGTGAAACCTACAAACAGTTCATAAAGAATCTGCGAAGTGCACTCACAGTTGGATCTCCGACAGTCTACAACATCCCCGTCTTGAAGAGCACAGCAACGGGGCCAGCAGGCTTTCTATTAGTTCACCTCACAAATTACAATGATGAATCCATCACGGTGGCAATAGACGTAGTGAACGTGTACGTGGTAGCATATGGAGCTGGAAACAATGCCTATTTCTTGAATGACGCTTCAACAGAAGCCAACAACCTTCTATTTAAAGGCCTCAAACATACAAGACTTCCTTACAAAGGCAATTACGATGGCCTTGAGACTGCTGCAGGAAAGATTTCAAGAGAGAAAATTGAACTTGGATTTTCTGAGATAAGCAGTGCCATTGGCAACATGTTCCACCACAATGCTGGTACTTCTGTCCCTAAAGCCTTCATTGTCATTATCGAAACTGTGTCTGAAGCTGCAAGATATAAATATATTGAGCAGAGAGTTTCTGAGAATGTGAAGACAAAGTTCTTGCCGGACCCTGCTTTCTTAAGCTTAGAGAATCGTTGGTCTGATTTATCCCAACAAGTTCAAATCGCACAGACGCGCGGAGGCCAGTTTGCTCGTGCTGTTGAGCTACGAACTGTTGGCAATAAACCAGTGCTCGTAACCAATGTTAGTTCGCCCATTGTCAAAGGAATTGCACTTCTGTTGTACTATAAACCCAGTGTTGCCGCCTAA

Protein sequence

MRLLGFYFLLALYVGGCVVEADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLKSTATGPAGFLLVHLTNYNDESITVAIDVVNVYVVAYGAGNNAYFLNDASTEANNLLFKGLKHTRLPYKGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETVSEAARYKYIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTVGNKPVLVTNVSSPIVKGIALLLYYKPSVAA

Homology

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BLAST of CmUC08G146230 vs. NCBI nr
Match: XP_022158192.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 433.0 bits (1112), Expect = 2.0e-117
Identity = 219/269 (81.41%), Postives = 241/269 (89.59%), Query Frame = 0


Query: 1   MRLLGFYFLLALYVGGCVVEADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLK 60
           MR L FYF+LAL  G    EADVSFSMLGATSETY+QFI+NLRS LT+ SP VY IPVL+
Sbjct: 1   MRFLPFYFVLALCFGALAAEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQ 60

Query: 61  STATGPAGFLLVHLTNYNDESITVAIDVVNVYVVAYGAGNNAYFLNDASTEANNLLFKGL 120
           STA G A F+LVHLTNYN ESITVAIDVVN+YVVAY AGNNAYFLNDAS EANN+LFKG+
Sbjct: 61  STAVGSARFILVHLTNYNTESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKGI 120

Query: 121 KHTRLPYKGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETV 180
           KH RLPYKGNYDGLETAAGKISREKI+LGFSEISS+IGNMFHHN GTS+ +AFIVII++V
Sbjct: 121 KHVRLPYKGNYDGLETAAGKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQSV 180

Query: 181 SEAARYKYIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTV 240
            EAAR+KYIEQRVSENVKTKF PDPAFLSLENRWSDLS+Q+QIAQ R G+FARA+ELRTV
Sbjct: 181 LEAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELRTV 240

Query: 241 GNKPVLVTNVSSPIVKGIALLLYYKPSVA 270
            NKP++VTNVSSPIVKGIALLLYYK  VA
Sbjct: 241 SNKPIIVTNVSSPIVKGIALLLYYKVRVA 269

[Close]

BLAST of CmUC08G146230 vs. NCBI nr
Match: XP_022158193.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 428.7 bits (1101), Expect = 3.7e-116
Identity = 221/268 (82.46%), Postives = 244/268 (91.04%), Query Frame = 0


Query: 1   MRLLGFYFLLALYVGGCVVEADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLK 60
           MRLL FYF LALY+G    EADVSFSMLG+TSETYKQFIKN+RSALTVGS  VY+IPVLK
Sbjct: 1   MRLLPFYFFLALYLGARAAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLK 60

Query: 61  STATGPAGFLLVHLTNYNDESITVAIDVVNVYVVAYGAGNNAYFLNDASTEANNLLFKGL 120
           +TATG A FLLVHLTNYN ESI VAIDVVNVYVVAY AGNNAYFLNDAS EA+ +LF+G+
Sbjct: 61  ATATGSARFLLVHLTNYNSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGI 120

Query: 121 KHTRLPYKGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETV 180
            H +L YKGNYDGLETAA KISREKI+LGFSEISS+IGNMFHHNAGTSVP+AFIVII++V
Sbjct: 121 NHVKLSYKGNYDGLETAA-KISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSV 180

Query: 181 SEAARYKYIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTV 240
           SEAAR+KYIEQRVSENVKT F PDPAFLSL+NRWSDLS+Q+QIAQ RGG+FARAVELRTV
Sbjct: 181 SEAARFKYIEQRVSENVKTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTV 240

Query: 241 GNKPVLVTNVSSPIVKGIALLLYYKPSV 269
            NKP+LVT+VSSP+VKGIALLLYYK SV
Sbjct: 241 SNKPILVTDVSSPVVKGIALLLYYKVSV 267

[Close]

BLAST of CmUC08G146230 vs. NCBI nr
Match: BAG12408.1 (karasurin-H precursor [Trichosanthes kirilowii var. japonica])

HSP 1 Score: 426.8 bits (1096), Expect = 1.4e-115
Identity = 216/269 (80.30%), Postives = 242/269 (89.96%), Query Frame = 0


Query: 1   MRLLGFYFLLALYVGGCVVEADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLK 60
           M+L+GFY +LALYVG  V EADV+FS+LG+TS TYKQFI+NLR+ALTVGSPTV NIPVLK
Sbjct: 1   MKLVGFYLVLALYVGAQVTEADVNFSLLGSTSATYKQFIQNLRAALTVGSPTVENIPVLK 60

Query: 61  STATGPAGFLLVHLTNYNDESITVAIDVVNVYVVAYGAGNNAYFLNDASTEANNLLFKGL 120
            TA G A FLLVHLTNYN ESITVA+DVVNVYVVAY AGNNAYFLNDAS EANN+LFKG+
Sbjct: 61  GTAAGLARFLLVHLTNYNGESITVAVDVVNVYVVAYRAGNNAYFLNDASAEANNVLFKGI 120

Query: 121 KHTRLPYKGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETV 180
            H RLPY GNYDGLETAAG+ISREKI LGFSEISSAIG+MFH+NAGTSVPKAFIVII+TV
Sbjct: 121 NHVRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFIVIIQTV 180

Query: 181 SEAARYKYIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTV 240
           SEAAR+KYI+QRVSENV+TKFLPDPAFLSLEN W  LS+Q+QIAQ RGG+FAR +ELRTV
Sbjct: 181 SEAARFKYIQQRVSENVQTKFLPDPAFLSLENAWGGLSEQIQIAQYRGGEFARPIELRTV 240

Query: 241 GNKPVLVTNVSSPIVKGIALLLYYKPSVA 270
            N P  VTNV+SP+VKG+ALLLYYK +VA
Sbjct: 241 SNTPTFVTNVNSPVVKGVALLLYYKVNVA 269

[Close]

BLAST of CmUC08G146230 vs. NCBI nr
Match: P98184.1 (RecName: Full=Ribosome-inactivating protein bryodin II; AltName: Full=BD2; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Bryonia dioica])

HSP 1 Score: 391.3 bits (1004), Expect = 6.5e-105
Identity = 200/269 (74.35%), Postives = 229/269 (85.13%), Query Frame = 0


Query: 1   MRLLGFYFLLALYVGGCVVE-ADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVL 60
           MR +GFY +LALYVG  V E  D++FS++GAT  TYK FI+NLR+ LTVG+P VY+IPVL
Sbjct: 1   MRSIGFYSVLALYVGAHVTEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVL 60

Query: 61  KSTATGPAGFLLVHLTNYNDESITVAIDVVNVYVVAYGAGNNAYFLNDASTEANNLLFKG 120
           ++ A G A F LV LTNYN ES+TVA+DVVNVYVVAY AGN AYFL DASTEANN+LF G
Sbjct: 61  RNAAAGLARFQLVTLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAG 120

Query: 121 LKHTRLPYKGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIET 180
           + H RLPY GNYDGLETAAG+ISRE IELGFSEISSAIGNMF HN GTSVP+AFIVII+T
Sbjct: 121 INHVRLPYGGNYDGLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQT 180

Query: 181 VSEAARYKYIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRT 240
           VSEAAR+KYIEQRVSENV TKF PDPAFLSL+N W  LS+Q+QIAQTRGG+FAR VELRT
Sbjct: 181 VSEAARFKYIEQRVSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRT 240

Query: 241 VGNKPVLVTNVSSPIVKGIALLLYYKPSV 269
           V N P  VTNV+SP+VKGIALLLY++ +V
Sbjct: 241 VSNTPTFVTNVNSPVVKGIALLLYFRVNV 269

[Close]

BLAST of CmUC08G146230 vs. NCBI nr
Match: XP_022158191.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 365.5 bits (937), Expect = 3.8e-97
Identity = 180/246 (73.17%), Postives = 217/246 (88.21%), Query Frame = 0


Query: 21  ADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLKSTATGPAGFLLVHLTNYNDE 80
           ++VSFS+LGAT +TY+QFI+NLR+ALT+ S  VYNIPVL +TA   A F+LVHLTNY +E
Sbjct: 35  SNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKNE 94

Query: 81  SITVAIDVVNVYVVAYGAGNNAYFLNDASTEANNLLFKGLKHTRLPYKGNYDGLETAAGK 140
           +ITVAIDVVNVY+VAY AGN AYFL DAS EA ++LFKG+K   LPYKGNYDGLETAAGK
Sbjct: 95  TITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETAAGK 154

Query: 141 ISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETVSEAARYKYIEQRVSENVKTK 200
           ISREKI+LGFSE+ SAIGNM+H+NAGTSVP+AFIV+I+T+SEAAR+KYIE +VS+NV+TK
Sbjct: 155 ISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVETK 214

Query: 201 FLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTVGNKPVLVTNVSSPIVKGIAL 260
           F PDP FLSLENRWSDLS+QVQIAQ R G+FAR +E+R+V NKP+LVTNV S +V+G+AL
Sbjct: 215 FKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMAL 274

Query: 261 LLYYKP 267
           LLY KP
Sbjct: 275 LLYSKP 280

[Close]

BLAST of CmUC08G146230 vs. ExPASy Swiss-Prot
Match: P98184 (Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 8.6e-108
Identity = 200/269 (74.35%), Postives = 229/269 (85.13%), Query Frame = 0


Query: 1   MRLLGFYFLLALYVGGCVVE-ADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVL 60
           MR +GFY +LALYVG  V E  D++FS++GAT  TYK FI+NLR+ LTVG+P VY+IPVL
Sbjct: 1   MRSIGFYSVLALYVGAHVTEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVL 60

Query: 61  KSTATGPAGFLLVHLTNYNDESITVAIDVVNVYVVAYGAGNNAYFLNDASTEANNLLFKG 120
           ++ A G A F LV LTNYN ES+TVA+DVVNVYVVAY AGN AYFL DASTEANN+LF G
Sbjct: 61  RNAAAGLARFQLVTLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAG 120

Query: 121 LKHTRLPYKGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIET 180
           + H RLPY GNYDGLETAAG+ISRE IELGFSEISSAIGNMF HN GTSVP+AFIVII+T
Sbjct: 121 INHVRLPYGGNYDGLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQT 180

Query: 181 VSEAARYKYIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRT 240
           VSEAAR+KYIEQRVSENV TKF PDPAFLSL+N W  LS+Q+QIAQTRGG+FAR VELRT
Sbjct: 181 VSEAARFKYIEQRVSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRT 240

Query: 241 VGNKPVLVTNVSSPIVKGIALLLYYKPSV 269
           V N P  VTNV+SP+VKGIALLLY++ +V
Sbjct: 241 VSNTPTFVTNVNSPVVKGIALLLYFRVNV 269

[Close]

BLAST of CmUC08G146230 vs. ExPASy Swiss-Prot
Match: P09989 (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 221.5 bits (563), Expect = 1.2e-56
Identity = 127/264 (48.11%), Postives = 173/264 (65.53%), Query Frame = 0


Query: 9   LLALYVGGCVVEADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLKSTATGPAG 68
           +L L++    VE DVSF + GATS +Y  FI NLR AL      +Y+IP+L+S+  G   
Sbjct: 11  ILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALP-NERKLYDIPLLRSSLPGSQR 70

Query: 69  FLLVHLTNYNDESITVAIDVVNVYVVAYGAGNNAYFLNDAS-TEANNLLFK-GLKHTRLP 128
           + L+HLTNY DE+I+VAIDV NVY++ Y AG+ +YF N+AS TEA   +FK  ++   LP
Sbjct: 71  YALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFKDAMRKVTLP 130

Query: 129 YKGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETVSEAARY 188
           Y GNY+ L+TAAGKI RE I LG   + SAI  +F++NA  S   A +V+I++ SEAARY
Sbjct: 131 YSGNYERLQTAAGKI-RENIPLGLPALDSAITTLFYYNA-NSAASALMVLIQSTSEAARY 190

Query: 189 KYIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTVGNKPVL 248
           K+IEQ++ + V   FLP  A +SLEN WS LS+Q+QIA T  GQF   V L    N+ V 
Sbjct: 191 KFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINAQNQRVT 250

Query: 249 VTNVSSPIVKGIALLLYYKPSVAA 271
           +TNV + +V     LL  + ++AA
Sbjct: 251 ITNVDAGVVTSNIALLLNRNNMAA 271

[Close]

BLAST of CmUC08G146230 vs. ExPASy Swiss-Prot
Match: P24478 (Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 220.7 bits (561), Expect = 2.0e-56
Identity = 133/273 (48.72%), Postives = 175/273 (64.10%), Query Frame = 0


Query: 1   MRLLGFYFL-LALYVGGCVVEADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVL 60
           +R L F  L L L++    VE DVSF + GATS +Y  FI NLR AL      +Y+IP+L
Sbjct: 2   IRFLVFSLLILTLFLTAPAVEGDVSFRLSGATSSSYGVFISNLRKALPY-ERKLYDIPLL 61

Query: 61  KSTATGPAGFLLVHLTNYNDESITVAIDVVNVYVVAYGAGNNAYFLNDAS-TEANNLLFK 120
           +ST  G   + L+HLTNY DE+I+VAIDV NVYV+ Y AG+ +YF N+AS TEA   +FK
Sbjct: 62  RSTLPGSQRYALIHLTNYADETISVAIDVTNVYVMGYRAGDTSYFFNEASATEAAKYVFK 121

Query: 121 GLKH-TRLPYKGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVII 180
             K    LPY GNY+ L+ AAGKI RE I LG   + SAI  +F++NA  S   A +V+I
Sbjct: 122 DAKRKVTLPYSGNYERLQIAAGKI-RENIPLGLPALDSAITTLFYYNA-NSAASALMVLI 181

Query: 181 ETVSEAARYKYIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVEL 240
           ++ SEAARYK+IEQ++ + V   FLP  A +SLEN WS LS+Q+QIA T  GQF   V L
Sbjct: 182 QSTSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFETPVVL 241

Query: 241 RTVGNKPVLVTNVSSPIVKGIALLLYYKPSVAA 271
               N+ V +TNV + +V     LL  + ++AA
Sbjct: 242 INAQNQRVTITNVDAGVVTSNIALLLNRNNMAA 271

[Close]

BLAST of CmUC08G146230 vs. ExPASy Swiss-Prot
Match: P56626 (Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina OX=50544 GN=TCA PE=1 SV=2)

HSP 1 Score: 219.9 bits (559), Expect = 3.4e-56
Identity = 128/266 (48.12%), Postives = 168/266 (63.16%), Query Frame = 0


Query: 7   YFLLALYVGGCVVEADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLKSTATGP 66
           +F LA+ +G      DVSF +  AT ++Y  FI  LR AL     TV  IP+L STA+G 
Sbjct: 5   FFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPT-QGTVCGIPLLPSTASGS 64

Query: 67  AGFLLVHLTNYNDESITVAIDVVNVYVVAYGAGNNAYFLNDASTEANNLLFKGLKHTRLP 126
             F   +LTNYNDE++TVA++V NVY+VAY A   +YF  D   EA  L+F G K  +LP
Sbjct: 65  QWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTVKLP 124

Query: 127 YKGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETVSEAARY 186
           Y GNYD L++  GK  R+ IELG   +SSAI NM +++   S   A +V+I+  +EAARY
Sbjct: 125 YSGNYDKLQSVVGK-QRDMIELGIPALSSAITNMVYYDY-QSTAAALLVLIQCTAEAARY 184

Query: 187 KYIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRG-GQFARAVELRTVGNKPV 246
           KYIEQ+VS ++ + F P+ A +SLEN+W  LS+Q+QIA   G GQF   VEL        
Sbjct: 185 KYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTRF 244

Query: 247 LVTNVSSPIVKG-IALLLYYKPSVAA 271
            VTN S+ +VKG I LLLYYK SV +
Sbjct: 245 SVTNTSAGVVKGNIKLLLYYKASVGS 267

[Close]

BLAST of CmUC08G146230 vs. ExPASy Swiss-Prot
Match: Q00465 (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.3e-55
Identity = 123/264 (46.59%), Postives = 180/264 (68.18%), Query Frame = 0


Query: 9   LLALYVGGCVVEADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLKSTATGPAG 68
           +LA++V    VEADV FS+ G++S +Y +FI +LR AL   + TVYNI +L S+A+G + 
Sbjct: 8   ILAIFVAASTVEADVRFSLSGSSSTSYSKFIGDLRKALP-SNGTVYNITLLLSSASGASR 67

Query: 69  FLLVHLTNYNDESITVAIDVVNVYVVAYGAGNNAYFLNDASTE-ANNLLFKGLKHTRLPY 128
           + L+ L+NY+ ++ITVA+DV NVY++ Y   + +YF N++  + A+  +FKG     LPY
Sbjct: 68  YTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPY 127

Query: 129 KGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETVSEAARYK 188
            GNY+ L+TAAGKI REKI LGF  + SAI  +FH+++ T+   AF+VII+T +EA+R+K
Sbjct: 128 SGNYEKLQTAAGKI-REKIPLGFPALDSAITTLFHYDS-TAAAAAFLVIIQTTAEASRFK 187

Query: 189 YIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTVGNKPVLV 248
           YIE ++ E +    +P  A +SLEN WS LS+Q+Q+AQT  G F   V +     + V +
Sbjct: 188 YIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDKGQRVEI 247

Query: 249 TNVSSPIV-KGIALLLYYKPSVAA 271
           TNV+S +V K I LLL YK +VAA
Sbjct: 248 TNVTSKVVTKNIQLLLNYKQNVAA 268

[Close]

BLAST of CmUC08G146230 vs. ExPASy TrEMBL
Match: A0A6J1DWK4 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 9.5e-118
Identity = 219/269 (81.41%), Postives = 241/269 (89.59%), Query Frame = 0


Query: 1   MRLLGFYFLLALYVGGCVVEADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLK 60
           MR L FYF+LAL  G    EADVSFSMLGATSETY+QFI+NLRS LT+ SP VY IPVL+
Sbjct: 1   MRFLPFYFVLALCFGALAAEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQ 60

Query: 61  STATGPAGFLLVHLTNYNDESITVAIDVVNVYVVAYGAGNNAYFLNDASTEANNLLFKGL 120
           STA G A F+LVHLTNYN ESITVAIDVVN+YVVAY AGNNAYFLNDAS EANN+LFKG+
Sbjct: 61  STAVGSARFILVHLTNYNTESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKGI 120

Query: 121 KHTRLPYKGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETV 180
           KH RLPYKGNYDGLETAAGKISREKI+LGFSEISS+IGNMFHHN GTS+ +AFIVII++V
Sbjct: 121 KHVRLPYKGNYDGLETAAGKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQSV 180

Query: 181 SEAARYKYIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTV 240
            EAAR+KYIEQRVSENVKTKF PDPAFLSLENRWSDLS+Q+QIAQ R G+FARA+ELRTV
Sbjct: 181 LEAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELRTV 240

Query: 241 GNKPVLVTNVSSPIVKGIALLLYYKPSVA 270
            NKP++VTNVSSPIVKGIALLLYYK  VA
Sbjct: 241 SNKPIIVTNVSSPIVKGIALLLYYKVRVA 269

[Close]

BLAST of CmUC08G146230 vs. ExPASy TrEMBL
Match: A0A6J1DVE7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 1.8e-116
Identity = 221/268 (82.46%), Postives = 244/268 (91.04%), Query Frame = 0


Query: 1   MRLLGFYFLLALYVGGCVVEADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLK 60
           MRLL FYF LALY+G    EADVSFSMLG+TSETYKQFIKN+RSALTVGS  VY+IPVLK
Sbjct: 1   MRLLPFYFFLALYLGARAAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLK 60

Query: 61  STATGPAGFLLVHLTNYNDESITVAIDVVNVYVVAYGAGNNAYFLNDASTEANNLLFKGL 120
           +TATG A FLLVHLTNYN ESI VAIDVVNVYVVAY AGNNAYFLNDAS EA+ +LF+G+
Sbjct: 61  ATATGSARFLLVHLTNYNSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGI 120

Query: 121 KHTRLPYKGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETV 180
            H +L YKGNYDGLETAA KISREKI+LGFSEISS+IGNMFHHNAGTSVP+AFIVII++V
Sbjct: 121 NHVKLSYKGNYDGLETAA-KISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSV 180

Query: 181 SEAARYKYIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTV 240
           SEAAR+KYIEQRVSENVKT F PDPAFLSL+NRWSDLS+Q+QIAQ RGG+FARAVELRTV
Sbjct: 181 SEAARFKYIEQRVSENVKTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTV 240

Query: 241 GNKPVLVTNVSSPIVKGIALLLYYKPSV 269
            NKP+LVT+VSSP+VKGIALLLYYK SV
Sbjct: 241 SNKPILVTDVSSPVVKGIALLLYYKVSV 267

[Close]

BLAST of CmUC08G146230 vs. ExPASy TrEMBL
Match: B1B626 (rRNA N-glycosidase OS=Trichosanthes kirilowii var. japonica OX=61011 PE=2 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 6.8e-116
Identity = 216/269 (80.30%), Postives = 242/269 (89.96%), Query Frame = 0


Query: 1   MRLLGFYFLLALYVGGCVVEADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLK 60
           M+L+GFY +LALYVG  V EADV+FS+LG+TS TYKQFI+NLR+ALTVGSPTV NIPVLK
Sbjct: 1   MKLVGFYLVLALYVGAQVTEADVNFSLLGSTSATYKQFIQNLRAALTVGSPTVENIPVLK 60

Query: 61  STATGPAGFLLVHLTNYNDESITVAIDVVNVYVVAYGAGNNAYFLNDASTEANNLLFKGL 120
            TA G A FLLVHLTNYN ESITVA+DVVNVYVVAY AGNNAYFLNDAS EANN+LFKG+
Sbjct: 61  GTAAGLARFLLVHLTNYNGESITVAVDVVNVYVVAYRAGNNAYFLNDASAEANNVLFKGI 120

Query: 121 KHTRLPYKGNYDGLETAAGKISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETV 180
            H RLPY GNYDGLETAAG+ISREKI LGFSEISSAIG+MFH+NAGTSVPKAFIVII+TV
Sbjct: 121 NHVRLPYGGNYDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFIVIIQTV 180

Query: 181 SEAARYKYIEQRVSENVKTKFLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTV 240
           SEAAR+KYI+QRVSENV+TKFLPDPAFLSLEN W  LS+Q+QIAQ RGG+FAR +ELRTV
Sbjct: 181 SEAARFKYIQQRVSENVQTKFLPDPAFLSLENAWGGLSEQIQIAQYRGGEFARPIELRTV 240

Query: 241 GNKPVLVTNVSSPIVKGIALLLYYKPSVA 270
            N P  VTNV+SP+VKG+ALLLYYK +VA
Sbjct: 241 SNTPTFVTNVNSPVVKGVALLLYYKVNVA 269

[Close]

BLAST of CmUC08G146230 vs. ExPASy TrEMBL
Match: A0A6J1E093 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 1.9e-97
Identity = 180/246 (73.17%), Postives = 217/246 (88.21%), Query Frame = 0


Query: 21  ADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLKSTATGPAGFLLVHLTNYNDE 80
           ++VSFS+LGAT +TY+QFI+NLR+ALT+ S  VYNIPVL +TA   A F+LVHLTNY +E
Sbjct: 35  SNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFILVHLTNYKNE 94

Query: 81  SITVAIDVVNVYVVAYGAGNNAYFLNDASTEANNLLFKGLKHTRLPYKGNYDGLETAAGK 140
           +ITVAIDVVNVY+VAY AGN AYFL DAS EA ++LFKG+K   LPYKGNYDGLETAAGK
Sbjct: 95  TITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQEILPYKGNYDGLETAAGK 154

Query: 141 ISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETVSEAARYKYIEQRVSENVKTK 200
           ISREKI+LGFSE+ SAIGNM+H+NAGTSVP+AFIV+I+T+SEAAR+KYIE +VS+NV+TK
Sbjct: 155 ISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIETKVSQNVETK 214

Query: 201 FLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTVGNKPVLVTNVSSPIVKGIAL 260
           F PDP FLSLENRWSDLS+QVQIAQ R G+FAR +E+R+V NKP+LVTNV S +V+G+AL
Sbjct: 215 FKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTNVKSKVVEGMAL 274

Query: 261 LLYYKP 267
           LLY KP
Sbjct: 275 LLYSKP 280

[Close]

BLAST of CmUC08G146230 vs. ExPASy TrEMBL
Match: A0A6J1IUB2 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 1.5e-86
Identity = 167/248 (67.34%), Postives = 206/248 (83.06%), Query Frame = 0


Query: 21  ADVSFSMLGATSETYKQFIKNLRSALTVGSPTVYNIPVLKSTATGPAGFLLVHLTNYNDE 80
           A++SFS+LGAT++TYKQFI +LR ALTV S  VY+IPVL +TA G A F+LV+LTNY  E
Sbjct: 34  ANLSFSLLGATAQTYKQFISDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGE 93

Query: 81  SITVAIDVVNVYVVAYGAGNNAYFLNDASTEANNLLFKGLKHTRLPYKGNYDGLETAAGK 140
           SITVAID V+VY+VAY AGN AYFL D S EA+++LFKG KH  L YKGNYD LE  AGK
Sbjct: 94  SITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGK 153

Query: 141 ISREKIELGFSEISSAIGNMFHHNAGTSVPKAFIVIIETVSEAARYKYIEQRVSENVKTK 200
           ISRE I+LGFSE+SS+IGNMFH+NAG SVPK+FIV+I+ +SEA+R+KYIE R  ENV++K
Sbjct: 154 ISRENIDLGFSELSSSIGNMFHYNAGYSVPKSFIVMIQMLSEASRFKYIEARNCENVESK 213

Query: 201 FLPDPAFLSLENRWSDLSQQVQIAQTRGGQFARAVELRTVGNKPVLVTNVSSPIVKGIAL 260
           F PDP +LSLENRWS+LS+QVQ AQ  GG+F   V++R+V NKPVLV++V+SP+VKG+AL
Sbjct: 214 FKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGMAL 273

Query: 261 LLYYKPSV 269
           LL  KP V
Sbjct: 274 LLCNKPGV 281

The following BLAST results are available for this feature:

BLAST of CmUC08G146230 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158192.1	2.0e-117	81.41	ribosome-inactivating protein bryodin II-like [Momordica charantia]	[more]
XP_022158193.1	3.7e-116	82.46	ribosome-inactivating protein bryodin II-like [Momordica charantia]	[more]
BAG12408.1	1.4e-115	80.30	karasurin-H precursor [Trichosanthes kirilowii var. japonica]	[more]
P98184.1	6.5e-105	74.35	RecName: Full=Ribosome-inactivating protein bryodin II; AltName: Full=BD2; AltNa...	[more]
XP_022158191.1	3.8e-97	73.17	ribosome-inactivating protein bryodin II-like [Momordica charantia]	[more]

BLAST of CmUC08G146230 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P98184	8.6e-108	74.35	Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1	[more]
P09989	1.2e-56	48.11	Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=...	[more]
P24478	2.0e-56	48.72	Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=...	[more]
P56626	3.4e-56	48.12	Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina OX=5...	[more]
Q00465	1.3e-55	46.59	Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]

BLAST of CmUC08G146230 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DWK4	9.5e-118	81.41	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1	[more]
A0A6J1DVE7	1.8e-116	82.46	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1	[more]
B1B626	6.8e-116	80.30	rRNA N-glycosidase OS=Trichosanthes kirilowii var. japonica OX=61011 PE=2 SV=1	[more]
A0A6J1E093	1.9e-97	73.17	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1	[more]
A0A6J1IUB2	1.5e-86	67.34	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1	[more]

BLAST of CmUC08G146230 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 205..222 score: 44.3 coord: 143..157 score: 44.04 coord: 33..46 score: 41.73 coord: 173..193 score: 54.51 coord: 81..96 score: 56.74
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 22..185 e-value: 4.3E-44 score: 152.2
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 31..222 e-value: 9.4E-32 score: 110.2
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 23..261
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 186..269 e-value: 3.8E-27 score: 96.0
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 23..261
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 177..193
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 22..267

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmUC08G146230.1	CmUC08G146230.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity