Homology
BLAST of CmUC07G130240 vs. NCBI nr
Match:
XP_023527145.1 (cucumisin-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1139.4 bits (2946), Expect = 0.0e+00
Identity = 575/754 (76.26%), Postives = 643/754 (85.28%), Query Frame = 0
Query: 5 MTRKSTTMYYSSLIFNLFLLGLFFSLLVSSRDSHKDGEKIYIVYMGRKLDDPDSARLHHR 64
MTRK TM SSL+FNL L LF S+L S+ SH D KIYIVYMG KL+DP SA LHHR
Sbjct: 1 MTRKGITM-CSSLLFNLLFLALFRSVLASTSHSHGDDRKIYIVYMGSKLEDPSSAHLHHR 60
Query: 65 AMLEEVVGSTFEPESVVYTYKRSFNGFAVKLTEEEAQNIAAKEGVVSVFPSKTKHIKTTR 124
AMLE+VVGSTF+P+SV+YTY RSFNGF V+LTEEEAQ IA+KEGVVSVF ++ H+ TTR
Sbjct: 61 AMLEQVVGSTFDPQSVLYTYTRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTR 120
Query: 125 SWDFLGFPQNVPRVKQVESNIVVGVFDSGIWPESPSFNDEGFGPAPPNWKGTCQASINFS 184
SWDFLGFPQNV RV Q+ESNIVVGV DSGIWPESPSFND+GFG P W+G+CQ S NF
Sbjct: 121 SWDFLGFPQNVGRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGSCQVS-NFR 180
Query: 185 CNRKIIGARAYLIDSPVPPGDVNSPRDIDGHGTHTASTVAGGLVSQASLYGLGLGTARGG 244
CNRK+IG RAY I P+ PGDV SPRD DGHGTHTASTVAGGLVSQASLYGLG+GTARGG
Sbjct: 181 CNRKLIGGRAYRIGRPILPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGIGTARGG 240
Query: 245 VPSARIAVYKICWKAVGCSDANILAAFDDAVADGVDIISLSVGGEEAEVYFRDPIAIGAF 304
VPSARIA YK+CWK GCSDA+ILAAFDDA+ADGVDI+SLSVG E YF DPIAIG+F
Sbjct: 241 VPSARIAAYKVCWKD-GCSDADILAAFDDAIADGVDIVSLSVGSSEPRPYFNDPIAIGSF 300
Query: 305 HASKNGILTSNSAGNEGPKYFTTPSVSPWLLSVAASSMDRKFVTQVQLGNGNIYQGISIN 364
HA KNGILTSNSAGNEGPK FTT S+SPWLLSVAAS MDRKFVT+VQLGNGNIY+GISIN
Sbjct: 301 HAIKNGILTSNSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYEGISIN 360
Query: 365 TFSMDYKYPLVYAGDVPNIGFHRSISRYCGNNSVNPELVRGKILLCDDVMSSSVISTFGS 424
TF M+ +YPLVYAGD+PN+GF+ S SRYC NSVNP L GKILLCD V+ SS+ S+FG+
Sbjct: 361 TFDMNLQYPLVYAGDIPNLGFNGSTSRYCEKNSVNPSLAAGKILLCDSVIPSSIFSSFGA 420
Query: 425 VAGVLMQAYSNDSARSFILPATVLHPQDGTHIYPYIRSTRTPTATIFKSIVARDTYAPVV 484
+AG+LMQAY+ D+ARS+ PA VLHP DG +Y YIRST PTATIFKS+ +D+ AP+V
Sbjct: 421 IAGILMQAYTRDNARSYPAPAAVLHPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPLV 480
Query: 485 VSFSSKGPNAITNDILKVKKTICRMPDLTGPGVEILAAWPPVAPIAGVRDSRSTHYNIIS 544
VSFSS+GPNA+TNDILK PDL+GPGVEILAAWPPVAPIAGV+DSRS+ YNIIS
Sbjct: 481 VSFSSRGPNALTNDILK--------PDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIIS 540
Query: 545 GTSMSCPHISGVAVYVKTFNPSWSPAAIKSALMTTASPMSATLNSQAEFAYGAGHVNPLK 604
GTSMSCPH++GVAVYVKTFNP+ SPAAIKSAL+TTASPMSA +SQAEFAYGAGHVNPLK
Sbjct: 541 GTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALITTASPMSARQHSQAEFAYGAGHVNPLK 600
Query: 605 AVDPGLVYDANESDYVKFLCGQGYNTAMVRHITGDNSACTSGNIGRVWDLNYPSFALSTA 664
A++PGLVYDANESDYV FLCGQGY+T+MVR ITGDNSACTSGNIGRVWDLNYPSFA+S +
Sbjct: 601 AINPGLVYDANESDYVSFLCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSIS 660
Query: 665 SPQTTVNQYFTRTLTNVAPGGYTYRARIFAPQGLRITANPPMLSFNGIGDRKSFTLIVRG 724
+ TVNQYFTRTLTNVA GG TYRA+I APQGL IT NP LSFNGIGDRKSF L V G
Sbjct: 661 GTR-TVNQYFTRTLTNVARGGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGG 720
Query: 725 TVNRKSIVSASLVWSDGVHSVRSPITIVVLTKSI 759
TV+ +SI SASL WSDGVHSVRSPIT+V LTKSI
Sbjct: 721 TVS-QSISSASLEWSDGVHSVRSPITVVALTKSI 741
BLAST of CmUC07G130240 vs. NCBI nr
Match:
BBK45496.1 (pre-pro-cucumisin like serine protease [Trichosanthes bracteata] >BBK45497.1 trichocucumisin [Trichosanthes bracteata])
HSP 1 Score: 1134.8 bits (2934), Expect = 0.0e+00
Identity = 575/751 (76.56%), Postives = 642/751 (85.49%), Query Frame = 0
Query: 8 KSTTMYYSSLIFNLFLLGLFFSLLVSSRDSHKDGEKIYIVYMGRKLDDPDSARLHHRAML 67
+S TM SS+IF L LL L SLL S DS DG KIYIVYMG KL+DPDSA LHHRAML
Sbjct: 3 RSITM--SSVIFKLVLLSLLSSLLASGFDSDDDGRKIYIVYMGNKLEDPDSAHLHHRAML 62
Query: 68 EEVVGSTFEPESVVYTYKRSFNGFAVKLTEEEAQNIAAKEGVVSVFPSKTKHIKTTRSWD 127
EEVVGSTF PESV+YTYKRSFNGFAVKLTEEEAQ IAAKEGVVSVF ++ HI TT SWD
Sbjct: 63 EEVVGSTFAPESVIYTYKRSFNGFAVKLTEEEAQKIAAKEGVVSVFLNQQNHIHTTNSWD 122
Query: 128 FLGFPQNVPRVKQVESNIVVGVFDSGIWPESPSFNDEGFGPAPPNWKGTCQASINFSCNR 187
FLGFPQ V R++QVESNIVVGV DSGIWPESPSF DEGFG AP +WKGTCQAS NFSCN
Sbjct: 123 FLGFPQAVARMRQVESNIVVGVLDSGIWPESPSFRDEGFGRAPSSWKGTCQASKNFSCNN 182
Query: 188 KIIGARAYLIDSPVPPGDVNSPRDIDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPS 247
KIIGARAY + +PPGDV+SPRD DGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPS
Sbjct: 183 KIIGARAYRVGGTLPPGDVSSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPS 242
Query: 248 ARIAVYKICWKAVGCSDANILAAFDDAVADGVDIISLSVGGEEAEVYFRDPIAIGAFHAS 307
ARIA YKICW + GCSDA+ILAAFDDA+ADGV IISLSVGG +A YF DPIAIGAFHA
Sbjct: 243 ARIAAYKICW-SDGCSDADILAAFDDAIADGVHIISLSVGGSQARPYFNDPIAIGAFHAM 302
Query: 308 KNGILTSNSAGNEGPKYFTTPSVSPWLLSVAASSMDRKFVTQVQLGNGNIYQGISINTFS 367
K+GILTSNSAGNEG K+FTT S+SPWLLSVAAS+ DRKFVT VQLGNG IYQG +INTF
Sbjct: 303 KHGILTSNSAGNEGSKFFTTTSLSPWLLSVAASTTDRKFVTHVQLGNGKIYQGTAINTFD 362
Query: 368 MDYKYPLVYAGDVPNIGFHRSISRYCGNNSVNPELVRGKILLCDDVMSSSVISTFGSVAG 427
M+ +YPLVYAG+VPN+GF+ S SR+C NNSVNP LV+GKILLCD ++ SSV S+FG++AG
Sbjct: 363 MNNQYPLVYAGEVPNVGFNGSTSRFCLNNSVNPYLVKGKILLCDSILPSSVFSSFGAIAG 422
Query: 428 VLMQAYSNDSARSFILPATVLHPQDGTHIYPYIRSTRTPTATIFKSIVARDTYAPVVVSF 487
VLMQ Y+ D+ARS +PATVLHPQDG ++Y YI ST TPTA I RD APVV+SF
Sbjct: 423 VLMQGYT-DNARSHSVPATVLHPQDGANVYRYIYSTSTPTARILSGTTVRDASAPVVISF 482
Query: 488 SSKGPNAITNDILKVKKTICRMPDLTGPGVEILAAWPPVAPIAGVRDSRSTHYNIISGTS 547
SS+GPNA+TND+LK PD+TGPGVEILAAWPP+API+GVR+SRST +NIISGTS
Sbjct: 483 SSRGPNALTNDLLK--------PDITGPGVEILAAWPPIAPISGVRESRSTLFNIISGTS 542
Query: 548 MSCPHISGVAVYVKTFNPSWSPAAIKSALMTTASPMSATLNSQAEFAYGAGHVNPLKAVD 607
MSCPH+S VAVYVKTFNPSWSPAAIKSALMTTASPM+A LNS+AEFAYG+GHVNPLKAV+
Sbjct: 543 MSCPHLSAVAVYVKTFNPSWSPAAIKSALMTTASPMTARLNSEAEFAYGSGHVNPLKAVN 602
Query: 608 PGLVYDANESDYVKFLCGQGYNTAMVRHITGDNSACTSGNIGRVWDLNYPSFALSTASPQ 667
PGLVYDA++SDYVKFLCGQGYNTAM+R ITGDNSACTS NIGRVWDLNYPSFAL+ + Q
Sbjct: 603 PGLVYDADQSDYVKFLCGQGYNTAMLRRITGDNSACTSANIGRVWDLNYPSFALAVSGYQ 662
Query: 668 TTVNQYFTRTLTNVAPGGYTYRARIFAPQGLRITANPPMLSFNGIGDRKSFTLIVRGTVN 727
++NQYFTRTLTNVA TYRA I PQGL IT NPP+LSF+G GDRKSFTL VRG+V+
Sbjct: 663 -SLNQYFTRTLTNVASQASTYRATISVPQGLTITVNPPVLSFSGKGDRKSFTLTVRGSVS 722
Query: 728 RKSIVSASLVWSDGVHSVRSPITIVVLTKSI 759
+ IVS SLVWSDGVHSVRSP+ +VVLTKS+
Sbjct: 723 -QPIVSGSLVWSDGVHSVRSPLVVVVLTKSV 739
BLAST of CmUC07G130240 vs. NCBI nr
Match:
KAG6600967.1 (hypothetical protein SDJN03_06200, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1126.7 bits (2913), Expect = 0.0e+00
Identity = 567/744 (76.21%), Postives = 635/744 (85.35%), Query Frame = 0
Query: 15 SSLIFNLFLLGLFFSLLVSSRDSHKDGEKIYIVYMGRKLDDPDSARLHHRAMLEEVVGST 74
SSL+ NL L LF S+L S+ SH D KIYIVYMG KL DP SA LHHRAMLE+VVGST
Sbjct: 3 SSLLLNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVGST 62
Query: 75 FEPESVVYTYKRSFNGFAVKLTEEEAQNIAAKEGVVSVFPSKTKHIKTTRSWDFLGFPQN 134
F+P+SV+YTY RSFNGF V+LTEEEAQ IA+KEGVVSVF ++ H+ TTRSWDF+GFPQN
Sbjct: 63 FDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFPQN 122
Query: 135 VPRVKQVESNIVVGVFDSGIWPESPSFNDEGFGPAPPNWKGTCQASINFSCNRKIIGARA 194
V RV+Q+ESNIVVGV DSGIWPESPSFND+GFG P W+G CQ S NFSCNRK+IG RA
Sbjct: 123 VGRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQDS-NFSCNRKLIGGRA 182
Query: 195 YLIDSPVPPGDVNSPRDIDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAVYK 254
Y I P+PPGDV SPRD DGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIA YK
Sbjct: 183 YHIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAYK 242
Query: 255 ICWKAVGCSDANILAAFDDAVADGVDIISLSVGGEEAEVYFRDPIAIGAFHASKNGILTS 314
+CW+ GCSDA+ILAAFDDA+ADGVDI+SLSVG + YF DPIAIG+FHA KNGILTS
Sbjct: 243 VCWED-GCSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKNGILTS 302
Query: 315 NSAGNEGPKYFTTPSVSPWLLSVAASSMDRKFVTQVQLGNGNIYQGISINTFSMDYKYPL 374
NSAGNEGPK FTT S+SPWLLSVAAS MDRKFVT+VQLGNGNIYQGISINTF+M+ ++PL
Sbjct: 303 NSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNMNLQHPL 362
Query: 375 VYAGDVPNIGFHRSISRYCGNNSVNPELVRGKILLCDDVMSSSVISTFGSVAGVLMQAYS 434
VYAGD+PN+GF+ S SRYC NSVNP L GKILLCD V+ S S+FG++AG+LMQAY+
Sbjct: 363 VYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAIAGILMQAYT 422
Query: 435 NDSARSFILPATVLHPQDGTHIYPYIRSTRTPTATIFKSIVARDTYAPVVVSFSSKGPNA 494
D+ARS+ PA VL P DG +Y YIRST PTATIFKS+ +D+ APVVVSFSS+GPNA
Sbjct: 423 RDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPNA 482
Query: 495 ITNDILKVKKTICRMPDLTGPGVEILAAWPPVAPIAGVRDSRSTHYNIISGTSMSCPHIS 554
+TNDILK PDL+GPGVEILAAWPPVAPIAGV+DSRS+ YNIISGTSMSCPH++
Sbjct: 483 LTNDILK--------PDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLT 542
Query: 555 GVAVYVKTFNPSWSPAAIKSALMTTASPMSATLNSQAEFAYGAGHVNPLKAVDPGLVYDA 614
GVAVYVKTFNP+ SPAAIKSALMTTASPMSA +SQAEFAYGAGHVNPLKA++PGLVYDA
Sbjct: 543 GVAVYVKTFNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDA 602
Query: 615 NESDYVKFLCGQGYNTAMVRHITGDNSACTSGNIGRVWDLNYPSFALSTASPQTTVNQYF 674
NESDYV FLCGQGY+T+MVR ITGDNSACTSGNIGRVWDLNYPSFA+S + + TVNQYF
Sbjct: 603 NESDYVSFLCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGTR-TVNQYF 662
Query: 675 TRTLTNVAPGGYTYRARIFAPQGLRITANPPMLSFNGIGDRKSFTLIVRGTVNRKSIVSA 734
TRTLTNVA GG TYRA+I APQGL IT NP LSFNGIGDRKSF L V GTV+ +SI SA
Sbjct: 663 TRTLTNVARGGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGTVS-QSISSA 722
Query: 735 SLVWSDGVHSVRSPITIVVLTKSI 759
SL WSDGVHSVRSPIT+V LTK+I
Sbjct: 723 SLEWSDGVHSVRSPITVVALTKTI 734
BLAST of CmUC07G130240 vs. NCBI nr
Match:
XP_022957348.1 (cucumisin-like [Cucurbita moschata])
HSP 1 Score: 1120.5 bits (2897), Expect = 0.0e+00
Identity = 569/754 (75.46%), Postives = 634/754 (84.08%), Query Frame = 0
Query: 5 MTRKSTTMYYSSLIFNLFLLGLFFSLLVSSRDSHKDGEKIYIVYMGRKLDDPDSARLHHR 64
M RK + M S LI NL L LF S+L S+ SH D +IYIVYMG KL DP SA LHHR
Sbjct: 1 MIRKGSDM-SSPLILNLLFLALFCSVLASTSHSHDDDREIYIVYMGSKLQDPSSAHLHHR 60
Query: 65 AMLEEVVGSTFEPESVVYTYKRSFNGFAVKLTEEEAQNIAAKEGVVSVFPSKTKHIKTTR 124
AMLE+VVGSTF+P+SV+YTY RSFNGF V LTEEEAQ IA+KEGVVSVF ++ H+ TTR
Sbjct: 61 AMLEQVVGSTFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTR 120
Query: 125 SWDFLGFPQNVPRVKQVESNIVVGVFDSGIWPESPSFNDEGFGPAPPNWKGTCQASINFS 184
SWDF+GFPQNV RV Q+ESNIVVGV DSGIWPESPSFND+GFG P W+G CQ S NFS
Sbjct: 121 SWDFMGFPQNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVS-NFS 180
Query: 185 CNRKIIGARAYLIDSPVPPGDVNSPRDIDGHGTHTASTVAGGLVSQASLYGLGLGTARGG 244
CNRK+IG RAY I P+ PGDV SPRD DGHGTHTASTVAGGLVSQASLYGLGLGTARGG
Sbjct: 181 CNRKLIGGRAYHIGRPIAPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGG 240
Query: 245 VPSARIAVYKICWKAVGCSDANILAAFDDAVADGVDIISLSVGGEEAEVYFRDPIAIGAF 304
VPSARIA YK+CW+ GC DA+ILAAFDDA+ADGVDI+SLSVG + YF DPIAIG+F
Sbjct: 241 VPSARIAAYKVCWED-GCWDADILAAFDDAIADGVDIVSLSVGSNKPRPYFNDPIAIGSF 300
Query: 305 HASKNGILTSNSAGNEGPKYFTTPSVSPWLLSVAASSMDRKFVTQVQLGNGNIYQGISIN 364
HA KNGILTSNSAGNEGPK FTT S+SPWLLSVAAS MDRKFVT+VQLGNGNIYQGISIN
Sbjct: 301 HAIKNGILTSNSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISIN 360
Query: 365 TFSMDYKYPLVYAGDVPNIGFHRSISRYCGNNSVNPELVRGKILLCDDVMSSSVISTFGS 424
TF+M+ ++PLVYAGD+PN+GF+ S SRYC NSVNP L GKILLCD V+ S +FG+
Sbjct: 361 TFNMNLQHPLVYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFFSFGA 420
Query: 425 VAGVLMQAYSNDSARSFILPATVLHPQDGTHIYPYIRSTRTPTATIFKSIVARDTYAPVV 484
+ G+LMQAY+ D+ARS+ PA VL P DG +Y YIRST PTATIFKS+ +D+ APVV
Sbjct: 421 IVGILMQAYTRDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVV 480
Query: 485 VSFSSKGPNAITNDILKVKKTICRMPDLTGPGVEILAAWPPVAPIAGVRDSRSTHYNIIS 544
VSFSS+GPNA+TNDILK PDL+GPGVEILAAWPPVAPIAGV+DSRS+ YNIIS
Sbjct: 481 VSFSSRGPNALTNDILK--------PDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIIS 540
Query: 545 GTSMSCPHISGVAVYVKTFNPSWSPAAIKSALMTTASPMSATLNSQAEFAYGAGHVNPLK 604
GTSMSCPH++GVAVYVKTFNP+ SPAAIKSALMTTASPMSA +SQAEFAYGAGHVNPLK
Sbjct: 541 GTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLK 600
Query: 605 AVDPGLVYDANESDYVKFLCGQGYNTAMVRHITGDNSACTSGNIGRVWDLNYPSFALSTA 664
A++PGLVYDANESDYV FLCGQGY+TAMVR ITGDNSACTSGNIGRVWDLNYPSFA+S +
Sbjct: 601 AINPGLVYDANESDYVSFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNYPSFAVSIS 660
Query: 665 SPQTTVNQYFTRTLTNVAPGGYTYRARIFAPQGLRITANPPMLSFNGIGDRKSFTLIVRG 724
+ TVNQYFTRTLTNVA GG TYRA+I APQGL IT NP LSFNGIGDRKSF L VRG
Sbjct: 661 GTR-TVNQYFTRTLTNVARGGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVRG 720
Query: 725 TVNRKSIVSASLVWSDGVHSVRSPITIVVLTKSI 759
TV+ +SI SASL WSDGVHSVRSPIT+V LTKSI
Sbjct: 721 TVS-QSISSASLEWSDGVHSVRSPITVVALTKSI 741
BLAST of CmUC07G130240 vs. NCBI nr
Match:
AEM42989.1 (cucumisin [Siraitia grosvenorii])
HSP 1 Score: 1088.6 bits (2814), Expect = 0.0e+00
Identity = 557/740 (75.27%), Postives = 617/740 (83.38%), Query Frame = 0
Query: 15 SSLIFNLFLLGLFFSLLVSSRDSHKDGEKIYIVYMGRKLDDPDSARLHHRAMLEEVVGST 74
SSL+F L L LF SLLVSS DS+ DG KIYIVYMG KL+D SA L+HRAMLEEVVGST
Sbjct: 7 SSLVFKLIFLSLFCSLLVSSSDSNDDGRKIYIVYMGSKLEDTASAHLYHRAMLEEVVGST 66
Query: 75 FEPESVVYTYKRSFNGFAVKLTEEEAQNIAAKEGVVSVFPSKTKHIKTTRSWDFLGFPQN 134
F PESV+YTYKRSFNGFAVKLTEEEA IAAKEGVVSVFPS+ H+ TTRSWDFLG QN
Sbjct: 67 FAPESVIYTYKRSFNGFAVKLTEEEALKIAAKEGVVSVFPSEKNHLHTTRSWDFLGISQN 126
Query: 135 VPRVKQVESNIVVGVFDSGIWPESPSFNDEGFGPAPPNWKGTCQASINFSCNRKIIGARA 194
VPRVKQVESNIVVGVFDSGIWPE+PSFND+GFGPAP NW+GTCQAS NF CNRKIIGARA
Sbjct: 127 VPRVKQVESNIVVGVFDSGIWPENPSFNDDGFGPAPANWRGTCQASTNFRCNRKIIGARA 186
Query: 195 YLIDSPVPPGDVNSPRDIDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAVYK 254
Y S +PPGDV SPRD DGHGTHTASTVAG LVSQASLYGLG+GTARGGVP ARIAVYK
Sbjct: 187 YR-SSTLPPGDVRSPRDTDGHGTHTASTVAGVLVSQASLYGLGVGTARGGVPPARIAVYK 246
Query: 255 ICWKAVGCSDANILAAFDDAVADGVDIISLSVGGEEAEVYFRDPIAIGAFHASKNGILTS 314
ICW + GCSDA+ILAAFDDA+ADGVDIISLSVGG+ + Y + IAIG+FHA K GILTS
Sbjct: 247 ICW-SDGCSDADILAAFDDAIADGVDIISLSVGGKVPQPYLYNSIAIGSFHAMKRGILTS 306
Query: 315 NSAGNEGPKYFTTPSVSPWLLSVAASSMDRKFVTQVQLGNGNIYQGISINTFSMDYKYPL 374
NSAGN GPK FT S+SPWL +VAASS DRKFVTQV LGNGN YQG+SINTF M +YPL
Sbjct: 307 NSAGNNGPKSFTVTSLSPWLPTVAASSSDRKFVTQVLLGNGNTYQGVSINTFDMRNQYPL 366
Query: 375 VYAGDVPNIGFHRSISRYCGNNSVNPELVRGKILLCDDVMSSSVISTFGSVAGVLMQAYS 434
+YAG+ P+IGF+ S SRYC +SV+P LVRGKILLCD +V ++FG AGVLMQ+ +
Sbjct: 367 IYAGNAPSIGFNSSTSRYCYEDSVDPNLVRGKILLCDSTFGPTVFASFGGAAGVLMQSNT 426
Query: 435 NDSARSFILPATVLHPQDGTHIYPYIRSTRTPTATIFKSIVARDTYAPVVVSFSSKGPNA 494
D A S+ LPA+VL P G +I Y+ STR PTATIFKS V RDT APVVVSFSS+GPN
Sbjct: 427 RDHASSYPLPASVLDPAGGNNIKRYMSSTRAPTATIFKSTVVRDTSAPVVVSFSSRGPNY 486
Query: 495 ITNDILKVKKTICRMPDLTGPGVEILAAWPPVAPIAGVRDSRSTHYNIISGTSMSCPHIS 554
+T+DILK PD T PGVEILAAWPPVAPI+GVRDSRS YNIISGTSMSCPH++
Sbjct: 487 VTHDILK--------PDSTAPGVEILAAWPPVAPISGVRDSRSALYNIISGTSMSCPHVT 546
Query: 555 GVAVYVKTFNPSWSPAAIKSALMTTASPMSATLNSQAEFAYGAGHVNPLKAVDPGLVYDA 614
+AV++KTF PSWSPAAIKSALMTTASPM+A NS AEFAYG+GHVNPLKAVDPGLVYDA
Sbjct: 547 AIAVHIKTFYPSWSPAAIKSALMTTASPMNARFNSDAEFAYGSGHVNPLKAVDPGLVYDA 606
Query: 615 NESDYVKFLCGQGYNTAMVRHITGDNSACTSGNIGRVWDLNYPSFALSTASPQTTVNQYF 674
+ESDYVKFLCG+GY TAMVR TGDNSACTSGNIGRVWDLNYPSFALS + Q T NQ F
Sbjct: 607 SESDYVKFLCGEGYTTAMVRSTTGDNSACTSGNIGRVWDLNYPSFALSISRSQ-TANQSF 666
Query: 675 TRTLTNVAPGGYTYRARIFAPQGLRITANPPMLSFNGIGDRKSFTLIVRGTVNRKSIVSA 734
RTLTNV G TYRA I APQGL I+ NP +LSFNGIGD+KSFTL VRGTV+ ++IVSA
Sbjct: 667 RRTLTNVVSGASTYRASISAPQGLSISVNPSVLSFNGIGDQKSFTLTVRGTVS-QAIVSA 726
Query: 735 SLVWSDGVHSVRSPITIVVL 755
SLVWSDG H+VRSPIT+ VL
Sbjct: 727 SLVWSDGSHNVRSPITVYVL 734
BLAST of CmUC07G130240 vs. ExPASy Swiss-Prot
Match:
Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)
HSP 1 Score: 1006.1 bits (2600), Expect = 2.1e-292
Identity = 516/742 (69.54%), Postives = 587/742 (79.11%), Query Frame = 0
Query: 15 SSLIFNLFLLGLFFSLLVSSR-DSHKDGEKIYIVYMGRKLDDPDSARLHHRAMLEEVVGS 74
SSLIF LF LFFS ++SR DS DG+ IYIVYMGRKL+DPDSA LHHRAMLE+VVGS
Sbjct: 3 SSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVVGS 62
Query: 75 TFEPESVVYTYKRSFNGFAVKLTEEEAQNIAAKEGVVSVFPSKTKHIKTTRSWDFLGFPQ 134
TF PESV++TYKRSFNGFAVKLTEEEA+ IA+ EGVVSVF ++ + TTRSWDFLGFP
Sbjct: 63 TFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFPL 122
Query: 135 NVPRVKQVESNIVVGVFDSGIWPESPSFNDEGFGPAPPNWKGTCQASINFSCNRKIIGAR 194
VPR QVESNIVVGV D+GIWPESPSF+DEGF P PP WKGTC+ S NF CNRKIIGAR
Sbjct: 123 TVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIGAR 182
Query: 195 AYLIDSPVPPGDVNSPRDIDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAVY 254
+Y I P+ PGDVN PRD +GHGTHTAST AGGLVSQA+LYGLGLGTARGGVP ARIA Y
Sbjct: 183 SYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAY 242
Query: 255 KICWKAVGCSDANILAAFDDAVADGVDIISLSVGGEEAEVYFRDPIAIGAFHASKNGILT 314
K+CW GCSD +ILAA+DDA+ADGVDIISLSVGG YF D IAIG+FHA + GILT
Sbjct: 243 KVCWND-GCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILT 302
Query: 315 SNSAGNEGPKYFTTPSVSPWLLSVAASSMDRKFVTQVQLGNGNIYQGISINTFSMDYKYP 374
SNSAGN GP +FTT S+SPWLLSVAAS+MDRKFVTQVQ+GNG +QG+SINTF Y YP
Sbjct: 303 SNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQY-YP 362
Query: 375 LVYAGDVPNIGFHRSISRYCGNNSVNPELVRGKILLCDDVMS-SSVISTFGSVAGVLMQA 434
LV D+PN GF +S SR+C + SVNP L++GKI++C+ + AGVLM +
Sbjct: 363 LVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTS 422
Query: 435 YSNDSARSFILPATVLHPQDGTHIYPYIRSTRTPTATIFKSIVARDTYAPVVVSFSSKGP 494
+ D A S+ LP++VL P D YI S R+P ATIFKS + APVVVSFSS+GP
Sbjct: 423 NTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGP 482
Query: 495 NAITNDILKVKKTICRMPDLTGPGVEILAAWPPVAPIAGVRDSRSTHYNIISGTSMSCPH 554
N T D++K PD++GPGVEILAAWP VAP+ G+R R+T +NIISGTSMSCPH
Sbjct: 483 NRATKDVIK--------PDISGPGVEILAAWPSVAPVGGIR--RNTLFNIISGTSMSCPH 542
Query: 555 ISGVAVYVKTFNPSWSPAAIKSALMTTASPMSATLNSQAEFAYGAGHVNPLKAVDPGLVY 614
I+G+A YVKT+NP+WSPAAIKSALMTTASPM+A N QAEFAYG+GHVNPLKAV PGLVY
Sbjct: 543 ITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVY 602
Query: 615 DANESDYVKFLCGQGYNTAMVRHITGDNSACTSGNIGRVWDLNYPSFALSTASPQTTVNQ 674
DANESDYVKFLCGQGYNT VR ITGD SACTSGN GRVWDLNYPSF LS SP T NQ
Sbjct: 603 DANESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLS-VSPSQTFNQ 662
Query: 675 YFTRTLTNVAPGGYTYRARIFAPQGLRITANPPMLSFNGIGDRKSFTLIVRGTVNRKSIV 734
YF RTLT+VAP TYRA I APQGL I+ NP +LSFNG+GDRKSFTL VRG++ + +V
Sbjct: 663 YFNRTLTSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSI-KGFVV 722
Query: 735 SASLVWSDGVHSVRSPITIVVL 755
SASLVWSDGVH VRSPITI L
Sbjct: 723 SASLVWSDGVHYVRSPITITSL 730
BLAST of CmUC07G130240 vs. ExPASy Swiss-Prot
Match:
Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)
HSP 1 Score: 619.4 bits (1596), Expect = 5.4e-176
Identity = 339/751 (45.14%), Postives = 468/751 (62.32%), Query Frame = 0
Query: 5 MTRKSTTMYYSSLIFNLFLLGLFFSLLVSSRDSHKDGEKIYIVYMGRKLDDPDSARLHHR 64
M + ST +Y L L F+ VS+ D ++ +YIVYMG + S HH
Sbjct: 1 MAKLSTPLY-------LICLAFIFTRDVSAND-YRQASSVYIVYMGTLPEIKYSPPSHHL 60
Query: 65 AMLEEVVGSTFEPESVVYTYKRSFNGFAVKLTEEEAQNIAAKEGVVSVFPSKTKHIKTTR 124
++L+++VG+ +V +YKRSFNGFA L++ E+Q + + VVSVFPSK+ + TTR
Sbjct: 61 SILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTR 120
Query: 125 SWDFLGFPQNVPRVKQVESNIVVGVFDSGIWPESPSFNDEGFGPAPPNWKGTCQASINFS 184
SWDF+GF + R ES+++VGV DSGIWPES SF+DEGFGP P WKG+C+ + F+
Sbjct: 121 SWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKFA 180
Query: 185 CNRKIIGARAYLIDSPVPPGDVNSPRDIDGHGTHTASTVAGGLVSQASLYGLGLGTARGG 244
CN K+IGAR Y +S RD +GHGTHTAST AG V AS YGL GTARGG
Sbjct: 181 CNNKLIGARFY-------NKFADSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGG 240
Query: 245 VPSARIAVYKICWKAVGCSDANILAAFDDAVADGVDIISLSVGGEEAEVYFRDPIAIGAF 304
VPSARIA YK+C+ C+D +ILAAFDDA+ADGVD+IS+S+ + +AIG+F
Sbjct: 241 VPSARIAAYKVCFNR--CNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSF 300
Query: 305 HASKNGILTSNSAGNEGPKYFTTPSVSPWLLSVAASSMDRKFVTQVQLGNGNIYQGISIN 364
HA GI+T+ SAGN GP + +VSPW+++VAAS DR+F+ +V LGNG GIS+N
Sbjct: 301 HAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVN 360
Query: 365 TFSMD-YKYPLVYAGDVPNIGFHRSISRYCGNNSVNPELVRGKILLCDDVMSSSVISTFG 424
TF+++ K+P+VY +V ++ + YC + V+ ELV+GKI+LCDD + G
Sbjct: 361 TFNLNGTKFPIVYGQNVSR-NCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAYLAG 420
Query: 425 SVAGVLMQAYSNDSARSFILPATVLHPQDGTHIYPYIRSTRTPTATIFKSIVARDTYAPV 484
++ ++ DSA PA+ L +D I YI S P A I ++ D AP
Sbjct: 421 AIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPY 480
Query: 485 VVSFSSKGPNAITNDILKVKKTICRMPDLTGPGVEILAAWPPVA---PIAGVRDSRSTHY 544
V SFSS+GP+ + ++LK PD++ PG+EILAA+ PVA D RS Y
Sbjct: 481 VPSFSSRGPSFVIQNLLK--------PDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRY 540
Query: 545 NIISGTSMSCPHISGVAVYVKTFNPSWSPAAIKSALMTTASPMSATLNSQAEFAYGAGHV 604
+++SGTSM+CPH++GVA YVK+F+P WSP+AIKSA+MTTA+PM+ N + EFAYG+G +
Sbjct: 541 SVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQI 600
Query: 605 NPLKAVDPGLVYDANESDYVKFLCGQGYNTAMVRHITGDNSACTSGNIGRVWDLNYPSFA 664
NP KA DPGLVY+ DY+K LC +G+++ + +G N C+ V DLNYP+
Sbjct: 601 NPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERT--EVKDLNYPTMT 660
Query: 665 LSTASPQTTVNQYFTRTLTNVAPGGYTYRARIFAPQ-GLRITANPPMLSFNGIGDRKSFT 724
+ S N F RT+TNV TY+A + Q L+I+ P +L F + ++KSF
Sbjct: 661 -TFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFV 720
Query: 725 LIVRG-TVNRKSIVSASLVWSDGVHSVRSPI 750
+ + G + S VS+S+VWSDG HSVRSPI
Sbjct: 721 VTISGKELKDGSFVSSSVVWSDGSHSVRSPI 722
BLAST of CmUC07G130240 vs. ExPASy Swiss-Prot
Match:
Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)
HSP 1 Score: 615.5 bits (1586), Expect = 7.9e-175
Identity = 342/742 (46.09%), Postives = 468/742 (63.07%), Query Frame = 0
Query: 23 LLGLFFSLLV---SSRDSHKDGEKIYIVYMGRKLDDPDSARL-HHRAMLEEVVGSTFEPE 82
LL F+LLV +S +++YIVYMG D + HH ++L++V G + +
Sbjct: 9 LLSCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGESSIQD 68
Query: 83 SVVYTYKRSFNGFAVKLTEEEAQNIAAKEGVVSVFPSKTKHIKTTRSWDFLGFPQ--NVP 142
+V YKRSFNGFA +LTE E + +A+ + VVSVFPSK +++TT SW+F+G +
Sbjct: 69 RLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTK 128
Query: 143 RVKQVESNIVVGVFDSGIWPESPSFNDEGFGPAPPNWKGTCQASINFSCNRKIIGARAYL 202
R +ES+ ++GV DSGI+PES SF+ +GFGP P WKG C+ NF+CN K+IGAR Y
Sbjct: 129 RNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARYY- 188
Query: 203 IDSPVPPGDVNSPRDIDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAVYKIC 262
+P G S RD GHG+HTAS AG V S YGLG GT RGGVP+ARIAVYK+C
Sbjct: 189 --TPKLEGFPESARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVC 248
Query: 263 WKAV-GCSDANILAAFDDAVADGVDIISLSVGGEEAEVYFRDPIAIGAFHASKNGILTSN 322
V C+ ILAAFDDA+AD VDII++S+G + + D +AIGAFHA GILT N
Sbjct: 249 DPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVN 308
Query: 323 SAGNEGPKYFTTPSVSPWLLSVAASSMDRKFVTQVQLGNGNIYQGISINTFSMD-YKYPL 382
AGN GP+ T S++PWL +VAAS+M+R F+T+V LGNG G S+N+F ++ KYPL
Sbjct: 309 GAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPL 368
Query: 383 VYAGDVPNIGFHRSISRYCGNNSVNPELVRGKILLCDDVMSSSVISTFGSVAGVLMQAYS 442
VY G + S + +C ++ + V+GKI+LCD + G+VA ++ Y
Sbjct: 369 VY-GKSASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIVRNPY- 428
Query: 443 NDSARSFILPATVLHPQDGTHIYPYIRSTRTPTATIFKSIVARDTYAPVVVSFSSKGPNA 502
D+A F P +VL D + Y+ ST+ P A + KS + APVV S+SS+GPN
Sbjct: 429 EDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNP 488
Query: 503 ITNDILKVKKTICRMPDLTGPGVEILAAWPPVAPIAGVRDSRSTHYNIISGTSMSCPHIS 562
+ +DILK PD+T PG EILAA+ P P D+R Y +ISGTSMSCPH++
Sbjct: 489 LIHDILK--------PDITAPGSEILAAYSPYVP-PSESDTRHVKYTVISGTSMSCPHVA 548
Query: 563 GVAVYVKTFNPSWSPAAIKSALMTTASPMSATL---NSQAEFAYGAGHVNPLKAVDPGLV 622
GVA Y+KTF+P WSP+ I+SA+MTTA PM+A+ N AEFAYGAGHV+P+ A+ PGLV
Sbjct: 549 GVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLV 608
Query: 623 YDANESDYVKFLCGQGYNTAMVRHITGDNSACTSGNIGRVW-DLNYPSFALSTASPQTTV 682
Y+AN+SD++ FLCG Y +R I+GD+S+CT + +LNYPS + + S
Sbjct: 609 YEANKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMS-AQVSGTKPF 668
Query: 683 NQYFTRTLTNVAPGGYTYRARIFAPQGLRITANPPMLSFNGIGDRKSFTLIVRGT-VNRK 742
F RT+TNV TY+A++ + L++ P +LS + ++KSFT+ V G +
Sbjct: 669 KVTFRRTVTNVGRPNATYKAKVVGSK-LKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAE 728
Query: 743 SIVSASLVWSDGVHSVRSPITI 752
++VSA L+WSDGVH VRSPI +
Sbjct: 729 NLVSAQLIWSDGVHFVRSPIVV 734
BLAST of CmUC07G130240 vs. ExPASy Swiss-Prot
Match:
Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)
HSP 1 Score: 610.9 bits (1574), Expect = 1.9e-173
Identity = 332/738 (44.99%), Postives = 461/738 (62.47%), Query Frame = 0
Query: 23 LLGLFFSLLVSSRDSHKDGEKIYIVYMGRKLDDPD-SARLHHRAMLEEVVGSTFEPESVV 82
LL L +SS + D +++YIVYMG D + H +L+EV G + +V
Sbjct: 10 LLSCLLVLFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGESSIEGRLV 69
Query: 83 YTYKRSFNGFAVKLTEEEAQNIAAKEGVVSVFPSKTKHIKTTRSWDFLGFPQNV--PRVK 142
+YKRSFNGFA +LTE E + +A GVVSVFP+K ++TT SWDF+G + + R
Sbjct: 70 RSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRNP 129
Query: 143 QVESNIVVGVFDSGIWPESPSFNDEGFGPAPPNWKGTCQASINFSCNRKIIGARAYLIDS 202
VES+ ++GV DSGI PES SF+D+GFGP P WKG C NF+CN K+IGAR Y +
Sbjct: 130 TVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDYTSE- 189
Query: 203 PVPPGDVNSPRDIDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAVYKICWKA 262
RD+DGHGTHTAST AG V AS +G+G GT RGGVP++R+A YK+C
Sbjct: 190 --------GTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVC-TP 249
Query: 263 VGCSDANILAAFDDAVADGVDIISLSVGGEEAEVYFRDPIAIGAFHASKNGILTSNSAGN 322
GCS +L+AFDDA+ADGVD+I++S+G + A ++ DPIAIGAFHA G+LT NSAGN
Sbjct: 250 TGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGN 309
Query: 323 EGPKYFTTPSVSPWLLSVAASSMDRKFVTQVQLGNGNIYQGISINTFSMDYK-YPLVYAG 382
GPK + V+PW+L+VAAS+ +R FVT+V LGNG G S+N + M K YPLVY
Sbjct: 310 SGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGK 369
Query: 383 DVPNIGFHRSISRYCGNNSVNPELVRGKILLCDDVMSSSVISTFGSVAGVLMQAYSNDSA 442
+ + C + V+ V+GKIL+C ++ + G+V G++ + D A
Sbjct: 370 SAASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESVGAV-GLIYRTPKPDVA 429
Query: 443 RSFILPATVLHPQDGTHIYPYIRSTRTPTATIFKSIVARDTYAPVVVSFSSKGPNAITND 502
LPA L +D + Y+ ST +P A + K+ + +PV+ SFSS+GPN I D
Sbjct: 430 FIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVD 489
Query: 503 ILKVKKTICRMPDLTGPGVEILAAWPPVAPIAGVRDSRSTHYNIISGTSMSCPHISGVAV 562
ILK PD+T PGVEILAA+ P A D+R Y+++SGTSMSCPH++GVA
Sbjct: 490 ILK--------PDITAPGVEILAAYSP-AGEPSQDDTRHVKYSVLSGTSMSCPHVAGVAA 549
Query: 563 YVKTFNPSWSPAAIKSALMTTASPMSATLN--SQAEFAYGAGHVNPLKAVDPGLVYDANE 622
YVKTFNP WSP+ I+SA+MTTA P++AT + EFAYG+GHV+P+ A +PGLVY+ ++
Sbjct: 550 YVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDK 609
Query: 623 SDYVKFLCGQGYNTAMVRHITGDNSACTSGNIGRVWDLNYPSFALSTASPQTTVNQYFTR 682
SD++ FLCG Y + +++ I+G+ C+ +LNYPS + + TT F R
Sbjct: 610 SDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTFNR 669
Query: 683 TLTNVAPGGYTYRARIFAPQG--LRITANPPMLSFNGIGDRKSFTLIVRGT-VNRKSIVS 742
TLTNV TY +++ A G L + P +LSF + +++SFT+ V G+ ++ + S
Sbjct: 670 TLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSS 727
Query: 743 ASLVWSDGVHSVRSPITI 752
A+L+WSDG H+VRSPI +
Sbjct: 730 ANLIWSDGTHNVRSPIVV 727
BLAST of CmUC07G130240 vs. ExPASy Swiss-Prot
Match:
Q9FIM5 (Subtilisin-like protease SBT4.9 OS=Arabidopsis thaliana OX=3702 GN=SBT4.9 PE=2 SV=1)
HSP 1 Score: 609.0 bits (1569), Expect = 7.4e-173
Identity = 343/747 (45.92%), Postives = 467/747 (62.52%), Query Frame = 0
Query: 28 FSLLVSSRDSHKDGEKIYIVYMGRKLDDPDSARL------HHRAMLEEVVGSTFEPESVV 87
F + VS+ +++Y+VYMG S+RL HH ++L+EV G + +V
Sbjct: 18 FVISVSAVTDDSQDKQVYVVYMG----SLPSSRLEYTPMSHHMSILQEVTGESSVEGRLV 77
Query: 88 YTYKRSFNGFAVKLTEEEAQNIAAKEGVVSVFPSKTKHIKTTRSWDFLGFPQ--NVPRVK 147
+YKRSFNGFA +LTE E + +A EGVVSVFP ++TT SWDFLG + N R
Sbjct: 78 RSYKRSFNGFAARLTESERERVAEMEGVVSVFPDINYKLQTTASWDFLGLKEGKNTKRNL 137
Query: 148 QVESNIVVGVFDSGIWPESPSFNDEGFGPAPPNWKGTCQASINFSCNRKIIGARAYLIDS 207
+ES+ ++G DSGIWPES SF+D+GFGP P WKG C A NF+CN K+IGAR Y +
Sbjct: 138 AIESDTIIGFIDSGIWPESESFSDKGFGPPPKKWKGVCSAGKNFTCNNKLIGARDYTNE- 197
Query: 208 PVPPGDVNSPRDIDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAVYKICWKA 267
RDI+GHGTHTAST AG V S YG+G GTARGGVP++RIA YK C
Sbjct: 198 --------GTRDIEGHGTHTASTAAGNAVKNTSFYGIGNGTARGGVPASRIAAYKAC-SE 257
Query: 268 VGCSDANILAAFDDAVADGVDIISLSVGGEEAEVYFRDPIAIGAFHASKNGILTSNSAGN 327
+GC+ ++L+AFDDA+ADGVD+IS+S+G Y DPIAIGAFHA GILT SAGN
Sbjct: 258 MGCTTESVLSAFDDAIADGVDLISISLGANLVRTYETDPIAIGAFHAMVKGILTVQSAGN 317
Query: 328 EGPKYFTTPSVSPWLLSVAASSMDRKFVTQVQLGNGNIYQGISINTFSMDYK-YPLVYAG 387
GP + SV+PW+L+VAAS+ +R FVT+V LGNG + G S+N F + K YPL Y G
Sbjct: 318 GGPNPGSVMSVAPWILTVAASNTNRGFVTKVVLGNGKTFVGKSLNAFDLKGKNYPL-YGG 377
Query: 388 DVPNIGFHRSISRYCGNNSVNPELVRGKILLCDDVMSSSVISTFGSVAGVLMQAYSNDSA 447
S + L+RGKIL+ +D +SS ++ VA + +D A
Sbjct: 378 ------------------STDGPLLRGKILVSEDKVSSEIV-----VANI--NENYHDYA 437
Query: 448 RSFILPATVLHPQDGTHIYPYIRSTRTPTATIFKSIVARDTYAPVVVSFSSKGPNAITND 507
ILP++ L D + Y+ ST++P T+ KS + AP V FSS+GPN I D
Sbjct: 438 YVSILPSSALSKDDFDSVISYVNSTKSPHGTVLKSEAIFNQAAPKVAGFSSRGPNTIAVD 497
Query: 508 ILKVKKTICRMPDLTGPGVEILAAWPPV-APIAGVRDSRSTHYNIISGTSMSCPHISGVA 567
ILK PD+T PGVEILAA+ P+ +P RD+R Y+++SGTSMSCPH++GVA
Sbjct: 498 ILK--------PDVTAPGVEILAAFSPLNSPAQDKRDNRHVKYSVLSGTSMSCPHVAGVA 557
Query: 568 VYVKTFNPSWSPAAIKSALMTTASPMSATLNSQA--EFAYGAGHVNPLKAVDPGLVYDAN 627
Y+KTF+P WSP+ I+SA+MTTA PM+AT + A EFAYGAGHV+P+ A++PGLVY+
Sbjct: 558 AYIKTFHPEWSPSMIQSAIMTTAWPMNATGTAVASTEFAYGAGHVDPIAAINPGLVYEIG 617
Query: 628 ESDYVKFLCGQGYNTAMVRHITGDNSACTSGNIGRVWDLNYPSFALSTASPQTTVNQYFT 687
+SD++ FLCG YN ++ I G+ CT + R +LNYPS + +++ F
Sbjct: 618 KSDHIAFLCGLNYNATSLKLIAGEAVTCTGKTLPR--NLNYPSMSAKLPKSESSFIVTFN 677
Query: 688 RTLTNVAPGGYTYRARIFAPQG--LRITANPPMLSFNGIGDRKSFTLIVRGT-VNRKSIV 747
RT+TNV TY+++I G L++ +P +LS + +++SFT+ V G+ ++ K
Sbjct: 678 RTVTNVGTPNSTYKSKIVLNHGSNLKVEVSPSVLSMKSVKEKQSFTVTVSGSNIDPKLPS 712
Query: 748 SASLVWSDGVHSVRSPITIVVLTKSIT 760
SA+L+WSDG H+VRSP IVV T S++
Sbjct: 738 SANLIWSDGTHNVRSP--IVVYTYSVS 712
BLAST of CmUC07G130240 vs. ExPASy TrEMBL
Match:
A0A4P2YW59 (Pre-pro-cucumisin like serine protease OS=Trichosanthes bracteata OX=486425 PE=2 SV=1)
HSP 1 Score: 1134.8 bits (2934), Expect = 0.0e+00
Identity = 575/751 (76.56%), Postives = 642/751 (85.49%), Query Frame = 0
Query: 8 KSTTMYYSSLIFNLFLLGLFFSLLVSSRDSHKDGEKIYIVYMGRKLDDPDSARLHHRAML 67
+S TM SS+IF L LL L SLL S DS DG KIYIVYMG KL+DPDSA LHHRAML
Sbjct: 3 RSITM--SSVIFKLVLLSLLSSLLASGFDSDDDGRKIYIVYMGNKLEDPDSAHLHHRAML 62
Query: 68 EEVVGSTFEPESVVYTYKRSFNGFAVKLTEEEAQNIAAKEGVVSVFPSKTKHIKTTRSWD 127
EEVVGSTF PESV+YTYKRSFNGFAVKLTEEEAQ IAAKEGVVSVF ++ HI TT SWD
Sbjct: 63 EEVVGSTFAPESVIYTYKRSFNGFAVKLTEEEAQKIAAKEGVVSVFLNQQNHIHTTNSWD 122
Query: 128 FLGFPQNVPRVKQVESNIVVGVFDSGIWPESPSFNDEGFGPAPPNWKGTCQASINFSCNR 187
FLGFPQ V R++QVESNIVVGV DSGIWPESPSF DEGFG AP +WKGTCQAS NFSCN
Sbjct: 123 FLGFPQAVARMRQVESNIVVGVLDSGIWPESPSFRDEGFGRAPSSWKGTCQASKNFSCNN 182
Query: 188 KIIGARAYLIDSPVPPGDVNSPRDIDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPS 247
KIIGARAY + +PPGDV+SPRD DGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPS
Sbjct: 183 KIIGARAYRVGGTLPPGDVSSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPS 242
Query: 248 ARIAVYKICWKAVGCSDANILAAFDDAVADGVDIISLSVGGEEAEVYFRDPIAIGAFHAS 307
ARIA YKICW + GCSDA+ILAAFDDA+ADGV IISLSVGG +A YF DPIAIGAFHA
Sbjct: 243 ARIAAYKICW-SDGCSDADILAAFDDAIADGVHIISLSVGGSQARPYFNDPIAIGAFHAM 302
Query: 308 KNGILTSNSAGNEGPKYFTTPSVSPWLLSVAASSMDRKFVTQVQLGNGNIYQGISINTFS 367
K+GILTSNSAGNEG K+FTT S+SPWLLSVAAS+ DRKFVT VQLGNG IYQG +INTF
Sbjct: 303 KHGILTSNSAGNEGSKFFTTTSLSPWLLSVAASTTDRKFVTHVQLGNGKIYQGTAINTFD 362
Query: 368 MDYKYPLVYAGDVPNIGFHRSISRYCGNNSVNPELVRGKILLCDDVMSSSVISTFGSVAG 427
M+ +YPLVYAG+VPN+GF+ S SR+C NNSVNP LV+GKILLCD ++ SSV S+FG++AG
Sbjct: 363 MNNQYPLVYAGEVPNVGFNGSTSRFCLNNSVNPYLVKGKILLCDSILPSSVFSSFGAIAG 422
Query: 428 VLMQAYSNDSARSFILPATVLHPQDGTHIYPYIRSTRTPTATIFKSIVARDTYAPVVVSF 487
VLMQ Y+ D+ARS +PATVLHPQDG ++Y YI ST TPTA I RD APVV+SF
Sbjct: 423 VLMQGYT-DNARSHSVPATVLHPQDGANVYRYIYSTSTPTARILSGTTVRDASAPVVISF 482
Query: 488 SSKGPNAITNDILKVKKTICRMPDLTGPGVEILAAWPPVAPIAGVRDSRSTHYNIISGTS 547
SS+GPNA+TND+LK PD+TGPGVEILAAWPP+API+GVR+SRST +NIISGTS
Sbjct: 483 SSRGPNALTNDLLK--------PDITGPGVEILAAWPPIAPISGVRESRSTLFNIISGTS 542
Query: 548 MSCPHISGVAVYVKTFNPSWSPAAIKSALMTTASPMSATLNSQAEFAYGAGHVNPLKAVD 607
MSCPH+S VAVYVKTFNPSWSPAAIKSALMTTASPM+A LNS+AEFAYG+GHVNPLKAV+
Sbjct: 543 MSCPHLSAVAVYVKTFNPSWSPAAIKSALMTTASPMTARLNSEAEFAYGSGHVNPLKAVN 602
Query: 608 PGLVYDANESDYVKFLCGQGYNTAMVRHITGDNSACTSGNIGRVWDLNYPSFALSTASPQ 667
PGLVYDA++SDYVKFLCGQGYNTAM+R ITGDNSACTS NIGRVWDLNYPSFAL+ + Q
Sbjct: 603 PGLVYDADQSDYVKFLCGQGYNTAMLRRITGDNSACTSANIGRVWDLNYPSFALAVSGYQ 662
Query: 668 TTVNQYFTRTLTNVAPGGYTYRARIFAPQGLRITANPPMLSFNGIGDRKSFTLIVRGTVN 727
++NQYFTRTLTNVA TYRA I PQGL IT NPP+LSF+G GDRKSFTL VRG+V+
Sbjct: 663 -SLNQYFTRTLTNVASQASTYRATISVPQGLTITVNPPVLSFSGKGDRKSFTLTVRGSVS 722
Query: 728 RKSIVSASLVWSDGVHSVRSPITIVVLTKSI 759
+ IVS SLVWSDGVHSVRSP+ +VVLTKS+
Sbjct: 723 -QPIVSGSLVWSDGVHSVRSPLVVVVLTKSV 739
BLAST of CmUC07G130240 vs. ExPASy TrEMBL
Match:
A0A6J1GZY5 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458774 PE=3 SV=1)
HSP 1 Score: 1120.5 bits (2897), Expect = 0.0e+00
Identity = 569/754 (75.46%), Postives = 634/754 (84.08%), Query Frame = 0
Query: 5 MTRKSTTMYYSSLIFNLFLLGLFFSLLVSSRDSHKDGEKIYIVYMGRKLDDPDSARLHHR 64
M RK + M S LI NL L LF S+L S+ SH D +IYIVYMG KL DP SA LHHR
Sbjct: 1 MIRKGSDM-SSPLILNLLFLALFCSVLASTSHSHDDDREIYIVYMGSKLQDPSSAHLHHR 60
Query: 65 AMLEEVVGSTFEPESVVYTYKRSFNGFAVKLTEEEAQNIAAKEGVVSVFPSKTKHIKTTR 124
AMLE+VVGSTF+P+SV+YTY RSFNGF V LTEEEAQ IA+KEGVVSVF ++ H+ TTR
Sbjct: 61 AMLEQVVGSTFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTR 120
Query: 125 SWDFLGFPQNVPRVKQVESNIVVGVFDSGIWPESPSFNDEGFGPAPPNWKGTCQASINFS 184
SWDF+GFPQNV RV Q+ESNIVVGV DSGIWPESPSFND+GFG P W+G CQ S NFS
Sbjct: 121 SWDFMGFPQNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVS-NFS 180
Query: 185 CNRKIIGARAYLIDSPVPPGDVNSPRDIDGHGTHTASTVAGGLVSQASLYGLGLGTARGG 244
CNRK+IG RAY I P+ PGDV SPRD DGHGTHTASTVAGGLVSQASLYGLGLGTARGG
Sbjct: 181 CNRKLIGGRAYHIGRPIAPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGG 240
Query: 245 VPSARIAVYKICWKAVGCSDANILAAFDDAVADGVDIISLSVGGEEAEVYFRDPIAIGAF 304
VPSARIA YK+CW+ GC DA+ILAAFDDA+ADGVDI+SLSVG + YF DPIAIG+F
Sbjct: 241 VPSARIAAYKVCWED-GCWDADILAAFDDAIADGVDIVSLSVGSNKPRPYFNDPIAIGSF 300
Query: 305 HASKNGILTSNSAGNEGPKYFTTPSVSPWLLSVAASSMDRKFVTQVQLGNGNIYQGISIN 364
HA KNGILTSNSAGNEGPK FTT S+SPWLLSVAAS MDRKFVT+VQLGNGNIYQGISIN
Sbjct: 301 HAIKNGILTSNSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISIN 360
Query: 365 TFSMDYKYPLVYAGDVPNIGFHRSISRYCGNNSVNPELVRGKILLCDDVMSSSVISTFGS 424
TF+M+ ++PLVYAGD+PN+GF+ S SRYC NSVNP L GKILLCD V+ S +FG+
Sbjct: 361 TFNMNLQHPLVYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFFSFGA 420
Query: 425 VAGVLMQAYSNDSARSFILPATVLHPQDGTHIYPYIRSTRTPTATIFKSIVARDTYAPVV 484
+ G+LMQAY+ D+ARS+ PA VL P DG +Y YIRST PTATIFKS+ +D+ APVV
Sbjct: 421 IVGILMQAYTRDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVV 480
Query: 485 VSFSSKGPNAITNDILKVKKTICRMPDLTGPGVEILAAWPPVAPIAGVRDSRSTHYNIIS 544
VSFSS+GPNA+TNDILK PDL+GPGVEILAAWPPVAPIAGV+DSRS+ YNIIS
Sbjct: 481 VSFSSRGPNALTNDILK--------PDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIIS 540
Query: 545 GTSMSCPHISGVAVYVKTFNPSWSPAAIKSALMTTASPMSATLNSQAEFAYGAGHVNPLK 604
GTSMSCPH++GVAVYVKTFNP+ SPAAIKSALMTTASPMSA +SQAEFAYGAGHVNPLK
Sbjct: 541 GTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLK 600
Query: 605 AVDPGLVYDANESDYVKFLCGQGYNTAMVRHITGDNSACTSGNIGRVWDLNYPSFALSTA 664
A++PGLVYDANESDYV FLCGQGY+TAMVR ITGDNSACTSGNIGRVWDLNYPSFA+S +
Sbjct: 601 AINPGLVYDANESDYVSFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNYPSFAVSIS 660
Query: 665 SPQTTVNQYFTRTLTNVAPGGYTYRARIFAPQGLRITANPPMLSFNGIGDRKSFTLIVRG 724
+ TVNQYFTRTLTNVA GG TYRA+I APQGL IT NP LSFNGIGDRKSF L VRG
Sbjct: 661 GTR-TVNQYFTRTLTNVARGGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVRG 720
Query: 725 TVNRKSIVSASLVWSDGVHSVRSPITIVVLTKSI 759
TV+ +SI SASL WSDGVHSVRSPIT+V LTKSI
Sbjct: 721 TVS-QSISSASLEWSDGVHSVRSPITVVALTKSI 741
BLAST of CmUC07G130240 vs. ExPASy TrEMBL
Match:
K7NBW1 (Cucumisin OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)
HSP 1 Score: 1088.6 bits (2814), Expect = 0.0e+00
Identity = 557/740 (75.27%), Postives = 617/740 (83.38%), Query Frame = 0
Query: 15 SSLIFNLFLLGLFFSLLVSSRDSHKDGEKIYIVYMGRKLDDPDSARLHHRAMLEEVVGST 74
SSL+F L L LF SLLVSS DS+ DG KIYIVYMG KL+D SA L+HRAMLEEVVGST
Sbjct: 7 SSLVFKLIFLSLFCSLLVSSSDSNDDGRKIYIVYMGSKLEDTASAHLYHRAMLEEVVGST 66
Query: 75 FEPESVVYTYKRSFNGFAVKLTEEEAQNIAAKEGVVSVFPSKTKHIKTTRSWDFLGFPQN 134
F PESV+YTYKRSFNGFAVKLTEEEA IAAKEGVVSVFPS+ H+ TTRSWDFLG QN
Sbjct: 67 FAPESVIYTYKRSFNGFAVKLTEEEALKIAAKEGVVSVFPSEKNHLHTTRSWDFLGISQN 126
Query: 135 VPRVKQVESNIVVGVFDSGIWPESPSFNDEGFGPAPPNWKGTCQASINFSCNRKIIGARA 194
VPRVKQVESNIVVGVFDSGIWPE+PSFND+GFGPAP NW+GTCQAS NF CNRKIIGARA
Sbjct: 127 VPRVKQVESNIVVGVFDSGIWPENPSFNDDGFGPAPANWRGTCQASTNFRCNRKIIGARA 186
Query: 195 YLIDSPVPPGDVNSPRDIDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAVYK 254
Y S +PPGDV SPRD DGHGTHTASTVAG LVSQASLYGLG+GTARGGVP ARIAVYK
Sbjct: 187 YR-SSTLPPGDVRSPRDTDGHGTHTASTVAGVLVSQASLYGLGVGTARGGVPPARIAVYK 246
Query: 255 ICWKAVGCSDANILAAFDDAVADGVDIISLSVGGEEAEVYFRDPIAIGAFHASKNGILTS 314
ICW + GCSDA+ILAAFDDA+ADGVDIISLSVGG+ + Y + IAIG+FHA K GILTS
Sbjct: 247 ICW-SDGCSDADILAAFDDAIADGVDIISLSVGGKVPQPYLYNSIAIGSFHAMKRGILTS 306
Query: 315 NSAGNEGPKYFTTPSVSPWLLSVAASSMDRKFVTQVQLGNGNIYQGISINTFSMDYKYPL 374
NSAGN GPK FT S+SPWL +VAASS DRKFVTQV LGNGN YQG+SINTF M +YPL
Sbjct: 307 NSAGNNGPKSFTVTSLSPWLPTVAASSSDRKFVTQVLLGNGNTYQGVSINTFDMRNQYPL 366
Query: 375 VYAGDVPNIGFHRSISRYCGNNSVNPELVRGKILLCDDVMSSSVISTFGSVAGVLMQAYS 434
+YAG+ P+IGF+ S SRYC +SV+P LVRGKILLCD +V ++FG AGVLMQ+ +
Sbjct: 367 IYAGNAPSIGFNSSTSRYCYEDSVDPNLVRGKILLCDSTFGPTVFASFGGAAGVLMQSNT 426
Query: 435 NDSARSFILPATVLHPQDGTHIYPYIRSTRTPTATIFKSIVARDTYAPVVVSFSSKGPNA 494
D A S+ LPA+VL P G +I Y+ STR PTATIFKS V RDT APVVVSFSS+GPN
Sbjct: 427 RDHASSYPLPASVLDPAGGNNIKRYMSSTRAPTATIFKSTVVRDTSAPVVVSFSSRGPNY 486
Query: 495 ITNDILKVKKTICRMPDLTGPGVEILAAWPPVAPIAGVRDSRSTHYNIISGTSMSCPHIS 554
+T+DILK PD T PGVEILAAWPPVAPI+GVRDSRS YNIISGTSMSCPH++
Sbjct: 487 VTHDILK--------PDSTAPGVEILAAWPPVAPISGVRDSRSALYNIISGTSMSCPHVT 546
Query: 555 GVAVYVKTFNPSWSPAAIKSALMTTASPMSATLNSQAEFAYGAGHVNPLKAVDPGLVYDA 614
+AV++KTF PSWSPAAIKSALMTTASPM+A NS AEFAYG+GHVNPLKAVDPGLVYDA
Sbjct: 547 AIAVHIKTFYPSWSPAAIKSALMTTASPMNARFNSDAEFAYGSGHVNPLKAVDPGLVYDA 606
Query: 615 NESDYVKFLCGQGYNTAMVRHITGDNSACTSGNIGRVWDLNYPSFALSTASPQTTVNQYF 674
+ESDYVKFLCG+GY TAMVR TGDNSACTSGNIGRVWDLNYPSFALS + Q T NQ F
Sbjct: 607 SESDYVKFLCGEGYTTAMVRSTTGDNSACTSGNIGRVWDLNYPSFALSISRSQ-TANQSF 666
Query: 675 TRTLTNVAPGGYTYRARIFAPQGLRITANPPMLSFNGIGDRKSFTLIVRGTVNRKSIVSA 734
RTLTNV G TYRA I APQGL I+ NP +LSFNGIGD+KSFTL VRGTV+ ++IVSA
Sbjct: 667 RRTLTNVVSGASTYRASISAPQGLSISVNPSVLSFNGIGDQKSFTLTVRGTVS-QAIVSA 726
Query: 735 SLVWSDGVHSVRSPITIVVL 755
SLVWSDG H+VRSPIT+ VL
Sbjct: 727 SLVWSDGSHNVRSPITVYVL 734
BLAST of CmUC07G130240 vs. ExPASy TrEMBL
Match:
A0A6J1H1P2 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458775 PE=3 SV=1)
HSP 1 Score: 1043.9 bits (2698), Expect = 3.3e-301
Identity = 532/738 (72.09%), Postives = 606/738 (82.11%), Query Frame = 0
Query: 15 SSLIFNLFLLGLFFSLLVSSRDSHKDGEKIYIVYMGRKLDDPDSARLHHRAMLEEVVGST 74
S LI NL L LF S+L S+ SH D KIYIVYMG KL DP SA LHHRAMLE+VVGST
Sbjct: 3 SPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVGST 62
Query: 75 FEPESVVYTYKRSFNGFAVKLTEEEAQNIAAKEGVVSVFPSKTKHIKTTRSWDFLGFPQN 134
F+P+SV+YTY RSFNGF V LTEEEAQ IA+KEGVVSVF ++ H+ TTRSWDF+GFPQN
Sbjct: 63 FDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFPQN 122
Query: 135 VPRVKQVESNIVVGVFDSGIWPESPSFNDEGFGPAPPNWKGTCQASINFSCNRKIIGARA 194
V RV Q+ESNIVVGV DSGIWPESPSFND+GFG P W+G CQ S NFSCNRKIIGARA
Sbjct: 123 VDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVS-NFSCNRKIIGARA 182
Query: 195 YLIDSPVPPGDVNSPRDIDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAVYK 254
Y I + P +VNSPRD +GHGTHTAS VAGGLVSQASLY LGLGTARGG PSARIAVYK
Sbjct: 183 YHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAVYK 242
Query: 255 ICWKAVGCSDANILAAFDDAVADGVDIISLSVGGEEAEVYFRDPIAIGAFHASKNGILTS 314
ICWK GCSDA+ILAAFDDA+ADGVD ISLSVG + YF DPIAIG+FHA K GILTS
Sbjct: 243 ICWKH-GCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILTS 302
Query: 315 NSAGNEGPKYFTTPSVSPWLLSVAASSMDRKFVTQVQLGNGNIYQGISINTF-SMDYKYP 374
NSAGNEGPK TT S+SPWLLSVAA++MDRKFVT+V+LG+G +QG+SINTF +M Y+YP
Sbjct: 303 NSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMVYQYP 362
Query: 375 LVYAGDVPNIGFHRSISRYCGNNSVNPELVRGKILLCDDVMSSSVISTFGSVAGVLMQAY 434
LVY GDVPN GF RSISRYC NSVNPE VRGK+LLC+ ++ S+ S+ G++ GV+MQ+
Sbjct: 363 LVYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAINGVIMQSG 422
Query: 435 SNDSARSFILPATVLHPQDGTHIYPYIRSTRTPTATIFKSIVARDTYAPVVVSFSSKGPN 494
+ D+A S+ LPA ++ QD I+ + R T TP A FKS +D APV+ SFSS+GPN
Sbjct: 423 TRDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSRGPN 482
Query: 495 AITNDILKVKKTICRMPDLTGPGVEILAAWPPVAPIAGVRDSRSTHYNIISGTSMSCPHI 554
++ N ILK PDL+GPGVEIL AWPPVAPIA V+DSRS+ YNIISGTSMSC HI
Sbjct: 483 SLANHILK--------PDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHI 542
Query: 555 SGVAVYVKTFNPSWSPAAIKSALMTTASPMSATLNSQAEFAYGAGHVNPLKAVDPGLVYD 614
+GVAVYVKTFNP+ SPAAIKSALMTTASPM+A+L+SQAEFAYGAGHVNPLKAV+PGLVYD
Sbjct: 543 TGVAVYVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYD 602
Query: 615 ANESDYVKFLCGQGYNTAMVRHITGDNSACTSGNIGRVWDLNYPSFALSTASPQTTVNQY 674
A+E++YVKFLCGQGY+TAMVR ITGDNSACTSGNIGRVWDLN PSFALS A+ +NQY
Sbjct: 603 ADENEYVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQY 662
Query: 675 FTRTLTNVAPGGYTYRARIFAPQGLRITANPPMLSFNGIGDRKSFTLIVRGTVNRKSIVS 734
FTRTLTNVA G TYRA I AP+GLRIT NP +LSFN IG+RKSF L V GTV+R+ +VS
Sbjct: 663 FTRTLTNVASSGSTYRATISAPKGLRITVNPCVLSFNRIGERKSFNLTVGGTVSRR-LVS 722
Query: 735 ASLVWSDGVHSVRSPITI 752
A LVWSDGVHSVRSPITI
Sbjct: 723 ACLVWSDGVHSVRSPITI 729
BLAST of CmUC07G130240 vs. ExPASy TrEMBL
Match:
A0A1S3CF86 (LOW QUALITY PROTEIN: cucumisin OS=Cucumis melo OX=3656 GN=LOC103500253 PE=3 SV=1)
HSP 1 Score: 1007.3 bits (2603), Expect = 3.4e-290
Identity = 517/742 (69.68%), Postives = 589/742 (79.38%), Query Frame = 0
Query: 15 SSLIFNLFLLGLFFSLLVSSR-DSHKDGEKIYIVYMGRKLDDPDSARLHHRAMLEEVVGS 74
SSLIF LF LFFS ++SR DS DG+ IYIVYMGRKL+DPDSA LHHRAMLE+VVGS
Sbjct: 3 SSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVVGS 62
Query: 75 TFEPESVVYTYKRSFNGFAVKLTEEEAQNIAAKEGVVSVFPSKTKHIKTTRSWDFLGFPQ 134
TF PESV++TYKRSFNGFAVKLTEEEA+ IA+ EGVVSVF ++ + TTRSWDFLGFP
Sbjct: 63 TFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFPL 122
Query: 135 NVPRVKQVESNIVVGVFDSGIWPESPSFNDEGFGPAPPNWKGTCQASINFSCNRKIIGAR 194
VPR QVESNIVVGV D+GIWPESPSF+DEGF P PP WKGTC+ S NF CNRKIIGAR
Sbjct: 123 TVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIGAR 182
Query: 195 AYLIDSPVPPGDVNSPRDIDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAVY 254
+Y I P+ PGDVN PRD +GHGTHTAST AGGLVSQA+LYGLGLGTARGGVP ARIA Y
Sbjct: 183 SYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAY 242
Query: 255 KICWKAVGCSDANILAAFDDAVADGVDIISLSVGGEEAEVYFRDPIAIGAFHASKNGILT 314
K+CW GCSDA+ILAA+DDA+ADGVDIISLSVGG YF D IAIG+FHA + GILT
Sbjct: 243 KVCWND-GCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILT 302
Query: 315 SNSAGNEGPKYFTTPSVSPWLLSVAASSMDRKFVTQVQLGNGNIYQGISINTFSMDYKYP 374
SNSAGN GP +FTT S+SPWLLSVAAS+MDRKFVTQVQ+GNG +QG+SINTF Y YP
Sbjct: 303 SNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQY-YP 362
Query: 375 LVYAGDVPNIGFHRSISRYCGNNSVNPELVRGKILLCDDVMS-SSVISTFGSVAGVLMQA 434
LV D+PN GF +S SR+C +NSV P+L++GKI++C+ + AGVLM +
Sbjct: 363 LVSGRDIPNPGFDKSTSRFCTDNSVXPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTS 422
Query: 435 YSNDSARSFILPATVLHPQDGTHIYPYIRSTRTPTATIFKSIVARDTYAPVVVSFSSKGP 494
+ D A S+ LP++VL P D YI S R+P ATIFKS + APVVVSFSS+GP
Sbjct: 423 NTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGP 482
Query: 495 NAITNDILKVKKTICRMPDLTGPGVEILAAWPPVAPIAGVRDSRSTHYNIISGTSMSCPH 554
N T D++K PD++GPGVEILAAWP VAP+ G+R R+T +NIISGTSMSCPH
Sbjct: 483 NRATKDVIK--------PDISGPGVEILAAWPSVAPVGGIR--RNTLFNIISGTSMSCPH 542
Query: 555 ISGVAVYVKTFNPSWSPAAIKSALMTTASPMSATLNSQAEFAYGAGHVNPLKAVDPGLVY 614
I+G+A YVKT+NP+WSPAAIKSALMTTASPM+A N QAEFAYG+GHVNPLKAV PGLVY
Sbjct: 543 ITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVY 602
Query: 615 DANESDYVKFLCGQGYNTAMVRHITGDNSACTSGNIGRVWDLNYPSFALSTASPQTTVNQ 674
DANESDYVKFLCGQGYNT VR ITGD SACT GN GRVWDLNYPSF LS SP T NQ
Sbjct: 603 DANESDYVKFLCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPSFGLS-VSPSKTFNQ 662
Query: 675 YFTRTLTNVAPGGYTYRARIFAPQGLRITANPPMLSFNGIGDRKSFTLIVRGTVNRKSIV 734
YF RTLT+VAP TYRA I APQGL I+ NP +LSFNG+GDRKSFTL VRG++ + +V
Sbjct: 663 YFNRTLTSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSI-KGFVV 722
Query: 735 SASLVWSDGVHSVRSPITIVVL 755
SASLVWSDGVHSVRSPITI L
Sbjct: 723 SASLVWSDGVHSVRSPITITSL 730
BLAST of CmUC07G130240 vs. TAIR 10
Match:
AT3G46850.1 (Subtilase family protein )
HSP 1 Score: 615.5 bits (1586), Expect = 5.6e-176
Identity = 342/742 (46.09%), Postives = 468/742 (63.07%), Query Frame = 0
Query: 23 LLGLFFSLLV---SSRDSHKDGEKIYIVYMGRKLDDPDSARL-HHRAMLEEVVGSTFEPE 82
LL F+LLV +S +++YIVYMG D + HH ++L++V G + +
Sbjct: 9 LLSCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGESSIQD 68
Query: 83 SVVYTYKRSFNGFAVKLTEEEAQNIAAKEGVVSVFPSKTKHIKTTRSWDFLGFPQ--NVP 142
+V YKRSFNGFA +LTE E + +A+ + VVSVFPSK +++TT SW+F+G +
Sbjct: 69 RLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTK 128
Query: 143 RVKQVESNIVVGVFDSGIWPESPSFNDEGFGPAPPNWKGTCQASINFSCNRKIIGARAYL 202
R +ES+ ++GV DSGI+PES SF+ +GFGP P WKG C+ NF+CN K+IGAR Y
Sbjct: 129 RNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARYY- 188
Query: 203 IDSPVPPGDVNSPRDIDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAVYKIC 262
+P G S RD GHG+HTAS AG V S YGLG GT RGGVP+ARIAVYK+C
Sbjct: 189 --TPKLEGFPESARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVC 248
Query: 263 WKAV-GCSDANILAAFDDAVADGVDIISLSVGGEEAEVYFRDPIAIGAFHASKNGILTSN 322
V C+ ILAAFDDA+AD VDII++S+G + + D +AIGAFHA GILT N
Sbjct: 249 DPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVN 308
Query: 323 SAGNEGPKYFTTPSVSPWLLSVAASSMDRKFVTQVQLGNGNIYQGISINTFSMD-YKYPL 382
AGN GP+ T S++PWL +VAAS+M+R F+T+V LGNG G S+N+F ++ KYPL
Sbjct: 309 GAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPL 368
Query: 383 VYAGDVPNIGFHRSISRYCGNNSVNPELVRGKILLCDDVMSSSVISTFGSVAGVLMQAYS 442
VY G + S + +C ++ + V+GKI+LCD + G+VA ++ Y
Sbjct: 369 VY-GKSASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIVRNPY- 428
Query: 443 NDSARSFILPATVLHPQDGTHIYPYIRSTRTPTATIFKSIVARDTYAPVVVSFSSKGPNA 502
D+A F P +VL D + Y+ ST+ P A + KS + APVV S+SS+GPN
Sbjct: 429 EDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNP 488
Query: 503 ITNDILKVKKTICRMPDLTGPGVEILAAWPPVAPIAGVRDSRSTHYNIISGTSMSCPHIS 562
+ +DILK PD+T PG EILAA+ P P D+R Y +ISGTSMSCPH++
Sbjct: 489 LIHDILK--------PDITAPGSEILAAYSPYVP-PSESDTRHVKYTVISGTSMSCPHVA 548
Query: 563 GVAVYVKTFNPSWSPAAIKSALMTTASPMSATL---NSQAEFAYGAGHVNPLKAVDPGLV 622
GVA Y+KTF+P WSP+ I+SA+MTTA PM+A+ N AEFAYGAGHV+P+ A+ PGLV
Sbjct: 549 GVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLV 608
Query: 623 YDANESDYVKFLCGQGYNTAMVRHITGDNSACTSGNIGRVW-DLNYPSFALSTASPQTTV 682
Y+AN+SD++ FLCG Y +R I+GD+S+CT + +LNYPS + + S
Sbjct: 609 YEANKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMS-AQVSGTKPF 668
Query: 683 NQYFTRTLTNVAPGGYTYRARIFAPQGLRITANPPMLSFNGIGDRKSFTLIVRGT-VNRK 742
F RT+TNV TY+A++ + L++ P +LS + ++KSFT+ V G +
Sbjct: 669 KVTFRRTVTNVGRPNATYKAKVVGSK-LKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAE 728
Query: 743 SIVSASLVWSDGVHSVRSPITI 752
++VSA L+WSDGVH VRSPI +
Sbjct: 729 NLVSAQLIWSDGVHFVRSPIVV 734
BLAST of CmUC07G130240 vs. TAIR 10
Match:
AT5G59120.1 (subtilase 4.13 )
HSP 1 Score: 610.9 bits (1574), Expect = 1.4e-174
Identity = 332/738 (44.99%), Postives = 461/738 (62.47%), Query Frame = 0
Query: 23 LLGLFFSLLVSSRDSHKDGEKIYIVYMGRKLDDPD-SARLHHRAMLEEVVGSTFEPESVV 82
LL L +SS + D +++YIVYMG D + H +L+EV G + +V
Sbjct: 10 LLSCLLVLFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGESSIEGRLV 69
Query: 83 YTYKRSFNGFAVKLTEEEAQNIAAKEGVVSVFPSKTKHIKTTRSWDFLGFPQNV--PRVK 142
+YKRSFNGFA +LTE E + +A GVVSVFP+K ++TT SWDF+G + + R
Sbjct: 70 RSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRNP 129
Query: 143 QVESNIVVGVFDSGIWPESPSFNDEGFGPAPPNWKGTCQASINFSCNRKIIGARAYLIDS 202
VES+ ++GV DSGI PES SF+D+GFGP P WKG C NF+CN K+IGAR Y +
Sbjct: 130 TVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDYTSE- 189
Query: 203 PVPPGDVNSPRDIDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAVYKICWKA 262
RD+DGHGTHTAST AG V AS +G+G GT RGGVP++R+A YK+C
Sbjct: 190 --------GTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVC-TP 249
Query: 263 VGCSDANILAAFDDAVADGVDIISLSVGGEEAEVYFRDPIAIGAFHASKNGILTSNSAGN 322
GCS +L+AFDDA+ADGVD+I++S+G + A ++ DPIAIGAFHA G+LT NSAGN
Sbjct: 250 TGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGN 309
Query: 323 EGPKYFTTPSVSPWLLSVAASSMDRKFVTQVQLGNGNIYQGISINTFSMDYK-YPLVYAG 382
GPK + V+PW+L+VAAS+ +R FVT+V LGNG G S+N + M K YPLVY
Sbjct: 310 SGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGK 369
Query: 383 DVPNIGFHRSISRYCGNNSVNPELVRGKILLCDDVMSSSVISTFGSVAGVLMQAYSNDSA 442
+ + C + V+ V+GKIL+C ++ + G+V G++ + D A
Sbjct: 370 SAASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESVGAV-GLIYRTPKPDVA 429
Query: 443 RSFILPATVLHPQDGTHIYPYIRSTRTPTATIFKSIVARDTYAPVVVSFSSKGPNAITND 502
LPA L +D + Y+ ST +P A + K+ + +PV+ SFSS+GPN I D
Sbjct: 430 FIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVD 489
Query: 503 ILKVKKTICRMPDLTGPGVEILAAWPPVAPIAGVRDSRSTHYNIISGTSMSCPHISGVAV 562
ILK PD+T PGVEILAA+ P A D+R Y+++SGTSMSCPH++GVA
Sbjct: 490 ILK--------PDITAPGVEILAAYSP-AGEPSQDDTRHVKYSVLSGTSMSCPHVAGVAA 549
Query: 563 YVKTFNPSWSPAAIKSALMTTASPMSATLN--SQAEFAYGAGHVNPLKAVDPGLVYDANE 622
YVKTFNP WSP+ I+SA+MTTA P++AT + EFAYG+GHV+P+ A +PGLVY+ ++
Sbjct: 550 YVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDK 609
Query: 623 SDYVKFLCGQGYNTAMVRHITGDNSACTSGNIGRVWDLNYPSFALSTASPQTTVNQYFTR 682
SD++ FLCG Y + +++ I+G+ C+ +LNYPS + + TT F R
Sbjct: 610 SDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTFNR 669
Query: 683 TLTNVAPGGYTYRARIFAPQG--LRITANPPMLSFNGIGDRKSFTLIVRGT-VNRKSIVS 742
TLTNV TY +++ A G L + P +LSF + +++SFT+ V G+ ++ + S
Sbjct: 670 TLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSS 727
Query: 743 ASLVWSDGVHSVRSPITI 752
A+L+WSDG H+VRSPI +
Sbjct: 730 ANLIWSDGTHNVRSPIVV 727
BLAST of CmUC07G130240 vs. TAIR 10
Match:
AT5G58840.1 (Subtilase family protein )
HSP 1 Score: 609.0 bits (1569), Expect = 5.2e-174
Identity = 343/747 (45.92%), Postives = 467/747 (62.52%), Query Frame = 0
Query: 28 FSLLVSSRDSHKDGEKIYIVYMGRKLDDPDSARL------HHRAMLEEVVGSTFEPESVV 87
F + VS+ +++Y+VYMG S+RL HH ++L+EV G + +V
Sbjct: 18 FVISVSAVTDDSQDKQVYVVYMG----SLPSSRLEYTPMSHHMSILQEVTGESSVEGRLV 77
Query: 88 YTYKRSFNGFAVKLTEEEAQNIAAKEGVVSVFPSKTKHIKTTRSWDFLGFPQ--NVPRVK 147
+YKRSFNGFA +LTE E + +A EGVVSVFP ++TT SWDFLG + N R
Sbjct: 78 RSYKRSFNGFAARLTESERERVAEMEGVVSVFPDINYKLQTTASWDFLGLKEGKNTKRNL 137
Query: 148 QVESNIVVGVFDSGIWPESPSFNDEGFGPAPPNWKGTCQASINFSCNRKIIGARAYLIDS 207
+ES+ ++G DSGIWPES SF+D+GFGP P WKG C A NF+CN K+IGAR Y +
Sbjct: 138 AIESDTIIGFIDSGIWPESESFSDKGFGPPPKKWKGVCSAGKNFTCNNKLIGARDYTNE- 197
Query: 208 PVPPGDVNSPRDIDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAVYKICWKA 267
RDI+GHGTHTAST AG V S YG+G GTARGGVP++RIA YK C
Sbjct: 198 --------GTRDIEGHGTHTASTAAGNAVKNTSFYGIGNGTARGGVPASRIAAYKAC-SE 257
Query: 268 VGCSDANILAAFDDAVADGVDIISLSVGGEEAEVYFRDPIAIGAFHASKNGILTSNSAGN 327
+GC+ ++L+AFDDA+ADGVD+IS+S+G Y DPIAIGAFHA GILT SAGN
Sbjct: 258 MGCTTESVLSAFDDAIADGVDLISISLGANLVRTYETDPIAIGAFHAMVKGILTVQSAGN 317
Query: 328 EGPKYFTTPSVSPWLLSVAASSMDRKFVTQVQLGNGNIYQGISINTFSMDYK-YPLVYAG 387
GP + SV+PW+L+VAAS+ +R FVT+V LGNG + G S+N F + K YPL Y G
Sbjct: 318 GGPNPGSVMSVAPWILTVAASNTNRGFVTKVVLGNGKTFVGKSLNAFDLKGKNYPL-YGG 377
Query: 388 DVPNIGFHRSISRYCGNNSVNPELVRGKILLCDDVMSSSVISTFGSVAGVLMQAYSNDSA 447
S + L+RGKIL+ +D +SS ++ VA + +D A
Sbjct: 378 ------------------STDGPLLRGKILVSEDKVSSEIV-----VANI--NENYHDYA 437
Query: 448 RSFILPATVLHPQDGTHIYPYIRSTRTPTATIFKSIVARDTYAPVVVSFSSKGPNAITND 507
ILP++ L D + Y+ ST++P T+ KS + AP V FSS+GPN I D
Sbjct: 438 YVSILPSSALSKDDFDSVISYVNSTKSPHGTVLKSEAIFNQAAPKVAGFSSRGPNTIAVD 497
Query: 508 ILKVKKTICRMPDLTGPGVEILAAWPPV-APIAGVRDSRSTHYNIISGTSMSCPHISGVA 567
ILK PD+T PGVEILAA+ P+ +P RD+R Y+++SGTSMSCPH++GVA
Sbjct: 498 ILK--------PDVTAPGVEILAAFSPLNSPAQDKRDNRHVKYSVLSGTSMSCPHVAGVA 557
Query: 568 VYVKTFNPSWSPAAIKSALMTTASPMSATLNSQA--EFAYGAGHVNPLKAVDPGLVYDAN 627
Y+KTF+P WSP+ I+SA+MTTA PM+AT + A EFAYGAGHV+P+ A++PGLVY+
Sbjct: 558 AYIKTFHPEWSPSMIQSAIMTTAWPMNATGTAVASTEFAYGAGHVDPIAAINPGLVYEIG 617
Query: 628 ESDYVKFLCGQGYNTAMVRHITGDNSACTSGNIGRVWDLNYPSFALSTASPQTTVNQYFT 687
+SD++ FLCG YN ++ I G+ CT + R +LNYPS + +++ F
Sbjct: 618 KSDHIAFLCGLNYNATSLKLIAGEAVTCTGKTLPR--NLNYPSMSAKLPKSESSFIVTFN 677
Query: 688 RTLTNVAPGGYTYRARIFAPQG--LRITANPPMLSFNGIGDRKSFTLIVRGT-VNRKSIV 747
RT+TNV TY+++I G L++ +P +LS + +++SFT+ V G+ ++ K
Sbjct: 678 RTVTNVGTPNSTYKSKIVLNHGSNLKVEVSPSVLSMKSVKEKQSFTVTVSGSNIDPKLPS 712
Query: 748 SASLVWSDGVHSVRSPITIVVLTKSIT 760
SA+L+WSDG H+VRSP IVV T S++
Sbjct: 738 SANLIWSDGTHNVRSP--IVVYTYSVS 712
BLAST of CmUC07G130240 vs. TAIR 10
Match:
AT3G46840.1 (Subtilase family protein )
HSP 1 Score: 606.7 bits (1563), Expect = 2.6e-173
Identity = 340/744 (45.70%), Postives = 474/744 (63.71%), Query Frame = 0
Query: 23 LLGLFFSLLV---SSRDSHKDGEKIYIVYMGRKLDDPDSARL-HHRAMLEEVVGSTFEPE 82
LL F+LLV +S D ++ YIVYMG D + HH ++L++V G + +
Sbjct: 10 LLSCIFALLVVSFASADKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQDVTGESSIED 69
Query: 83 SVVYTYKRSFNGFAVKLTEEEAQNIAAKEGVVSVFPSKTKHIKTTRSWDFLGFPQN--VP 142
+V YKRSFNGFA +LT+ E + +A+ + VVSVFP+K ++TT SW+F+G ++
Sbjct: 70 RLVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFMGLKESKRTK 129
Query: 143 RVKQVESNIVVGVFDSGIWPESPSFNDEGFGPAPPNWKGTCQASINFSCNRKIIGARAYL 202
R +ES+ ++GV DSGI+PES SF+ +GFGP P WKG C+ NF+ N K+IGAR Y
Sbjct: 130 RNTIIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLIGARYY- 189
Query: 203 IDSPVPPGDVNSPRDIDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAVYKIC 262
+P G S RD GHG+HTAST AG V S YGLG GTARGGVP+ARIAVYK+C
Sbjct: 190 --TPKLEGFPESARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVPAARIAVYKVC 249
Query: 263 WKAV-GCSDANILAAFDDAVADGVDIISLSVGGEEAEVYFRDPIAIGAFHASKNGILTSN 322
V GC+ ILAAFDDA+AD VDII++S+GG+ + + DPIAIGAFHA GIL N
Sbjct: 250 DPGVDGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFHAMAKGILIVN 309
Query: 323 SAGNEGPKYFTTPSVSPWLLSVAASSMDRKFVTQVQLGNGNIYQGISINTFSMD-YKYPL 382
SAGN GP+ T S++PW+ +VAAS+ +R FVT+V LGNG G S+N+F ++ KYPL
Sbjct: 310 SAGNSGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVNSFDLNGKKYPL 369
Query: 383 VYAGDVPNIGFHRSISRYCGNNSVNPELVRGKILLCDDVMSSSVISTFGSVAGVLMQAYS 442
VY G + + + +C ++ + V+GKI+LCD + G++A + ++++
Sbjct: 370 VY-GKSASSSCGAASAGFCSPGCLDSKRVKGKIVLCDSPQNPDEAQAMGAIASI-VRSHR 429
Query: 443 NDSARSFILPATVLHPQDGTHIYPYIRSTRTPTATIFKSIVARDTYAPVVVSFSSKGPNA 502
D A F P +VL D + Y+ ST+ P A + KS + APVV S+ S+GPN
Sbjct: 430 TDVASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRGPNT 489
Query: 503 ITNDILKVKKTICRMPDLTGPGVEILAAWPPVAPIAGVRDSRSTHYNIISGTSMSCPHIS 562
I DILK PD+T PG EI+AA+ P AP + D+R Y++ +GTSMSCPH++
Sbjct: 490 IIPDILK--------PDITAPGSEIVAAYSPDAP-PSISDTRRVKYSVDTGTSMSCPHVA 549
Query: 563 GVAVYVKTFNPSWSPAAIKSALMTTASPMSAT---LNSQAEFAYGAGHVNPLKAVDPGLV 622
GVA Y+K+F+P WSP+ I+SA+MTTA PM+A+ N AEFAYGAGHV+P+ A+ PGLV
Sbjct: 550 GVAAYLKSFHPRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITAIHPGLV 609
Query: 623 YDANESDYVKFLCGQGYNTAMVRHITGDNSACTSGNIGRV-WDLNYPSFA--LSTASPQT 682
Y+AN+SD++ FLCG Y +R I+GD+S+CT + +LNYPS +S A P
Sbjct: 610 YEANKSDHIAFLCGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYPSMTAQVSAAKPFK 669
Query: 683 TVNQYFTRTLTNVAPGGYTYRARIFAPQGLRITANPPMLSFNGIGDRKSFTLIVRGT-VN 742
+ F RT+TNV TY+A++ + L++ P +LS + ++KSFT+ G
Sbjct: 670 VI---FRRTVTNVGRPNATYKAKVVGSK-LKVKVVPAVLSLKSLYEKKSFTVTASGAGPK 729
Query: 743 RKSIVSASLVWSDGVHSVRSPITI 752
+++VSA L+WSDGVH VRSPI +
Sbjct: 730 AENLVSAQLIWSDGVHFVRSPIVV 734
BLAST of CmUC07G130240 vs. TAIR 10
Match:
AT5G59190.1 (subtilase family protein )
HSP 1 Score: 603.6 bits (1555), Expect = 2.2e-172
Identity = 322/694 (46.40%), Postives = 443/694 (63.83%), Query Frame = 0
Query: 62 HHRAMLEEVVGSTFEPESVVYTYKRSFNGFAVKLTEEEAQNIAAKEGVVSVFPSKTKHIK 121
HH ++L+++VG+ +V +YKRSFNGFA L++ E+Q + + VVSVFPSK+ +
Sbjct: 14 HHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELT 73
Query: 122 TTRSWDFLGFPQNVPRVKQVESNIVVGVFDSGIWPESPSFNDEGFGPAPPNWKGTCQASI 181
TTRSWDF+GF + R ES+++VGV DSGIWPES SF+DEGFGP P WKG+C+ +
Sbjct: 74 TTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGL 133
Query: 182 NFSCNRKIIGARAYLIDSPVPPGDVNSPRDIDGHGTHTASTVAGGLVSQASLYGLGLGTA 241
F+CN K+IGAR Y +S RD +GHGTHTAST AG V AS YGL GTA
Sbjct: 134 KFACNNKLIGARFY-------NKFADSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTA 193
Query: 242 RGGVPSARIAVYKICWKAVGCSDANILAAFDDAVADGVDIISLSVGGEEAEVYFRDPIAI 301
RGGVPSARIA YK+C+ C+D +ILAAFDDA+ADGVD+IS+S+ + +AI
Sbjct: 194 RGGVPSARIAAYKVCFNR--CNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAI 253
Query: 302 GAFHASKNGILTSNSAGNEGPKYFTTPSVSPWLLSVAASSMDRKFVTQVQLGNGNIYQGI 361
G+FHA GI+T+ SAGN GP + +VSPW+++VAAS DR+F+ +V LGNG GI
Sbjct: 254 GSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGI 313
Query: 362 SINTFSMD-YKYPLVYAGDVPNIGFHRSISRYCGNNSVNPELVRGKILLCDDVMSSSVIS 421
S+NTF+++ K+P+VY +V ++ + YC + V+ ELV+GKI+LCDD +
Sbjct: 314 SVNTFNLNGTKFPIVYGQNVSR-NCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAY 373
Query: 422 TFGSVAGVLMQAYSNDSARSFILPATVLHPQDGTHIYPYIRSTRTPTATIFKSIVARDTY 481
G++ ++ DSA PA+ L +D I YI S P A I ++ D
Sbjct: 374 LAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDRE 433
Query: 482 APVVVSFSSKGPNAITNDILKVKKTICRMPDLTGPGVEILAAWPPVA---PIAGVRDSRS 541
AP V SFSS+GP+ + ++LK PD++ PG+EILAA+ PVA D RS
Sbjct: 434 APYVPSFSSRGPSFVIQNLLK--------PDVSAPGLEILAAFSPVASPSSFLNPEDKRS 493
Query: 542 THYNIISGTSMSCPHISGVAVYVKTFNPSWSPAAIKSALMTTASPMSATLNSQAEFAYGA 601
Y+++SGTSM+CPH++GVA YVK+F+P WSP+AIKSA+MTTA+PM+ N + EFAYG+
Sbjct: 494 VRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGS 553
Query: 602 GHVNPLKAVDPGLVYDANESDYVKFLCGQGYNTAMVRHITGDNSACTSGNIGRVWDLNYP 661
G +NP KA DPGLVY+ DY+K LC +G+++ + +G N C+ V DLNYP
Sbjct: 554 GQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERT--EVKDLNYP 613
Query: 662 SFALSTASPQTTVNQYFTRTLTNVAPGGYTYRARIFAPQ-GLRITANPPMLSFNGIGDRK 721
+ + S N F RT+TNV TY+A + Q L+I+ P +L F + ++K
Sbjct: 614 TMT-TFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKK 673
Query: 722 SFTLIVRG-TVNRKSIVSASLVWSDGVHSVRSPI 750
SF + + G + S VS+S+VWSDG HSVRSPI
Sbjct: 674 SFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPI 686
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_023527145.1 | 0.0e+00 | 76.26 | cucumisin-like [Cucurbita pepo subsp. pepo] | [more] |
| BBK45496.1 | 0.0e+00 | 76.56 | pre-pro-cucumisin like serine protease [Trichosanthes bracteata] >BBK45497.1 tri... | [more] |
| KAG6600967.1 | 0.0e+00 | 76.21 | hypothetical protein SDJN03_06200, partial [Cucurbita argyrosperma subsp. sorori... | [more] |
| XP_022957348.1 | 0.0e+00 | 75.46 | cucumisin-like [Cucurbita moschata] | [more] |
| AEM42989.1 | 0.0e+00 | 75.27 | cucumisin [Siraitia grosvenorii] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q39547 | 2.1e-292 | 69.54 | Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1 | [more] |
| Q9FIF8 | 5.4e-176 | 45.14 | Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... | [more] |
| Q9STF7 | 7.9e-175 | 46.09 | Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 S... | [more] |
| Q9FIG2 | 1.9e-173 | 44.99 | Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2... | [more] |
| Q9FIM5 | 7.4e-173 | 45.92 | Subtilisin-like protease SBT4.9 OS=Arabidopsis thaliana OX=3702 GN=SBT4.9 PE=2 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A4P2YW59 | 0.0e+00 | 76.56 | Pre-pro-cucumisin like serine protease OS=Trichosanthes bracteata OX=486425 PE=2... | [more] |
| A0A6J1GZY5 | 0.0e+00 | 75.46 | cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458774 PE=3 SV=1 | [more] |
| K7NBW1 | 0.0e+00 | 75.27 | Cucumisin OS=Siraitia grosvenorii OX=190515 PE=2 SV=1 | [more] |
| A0A6J1H1P2 | 3.3e-301 | 72.09 | cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458775 PE=3 SV=1 | [more] |
| A0A1S3CF86 | 3.4e-290 | 69.68 | LOW QUALITY PROTEIN: cucumisin OS=Cucumis melo OX=3656 GN=LOC103500253 PE=3 SV=1 | [more] |
