CmUC07G130220 (gene) Watermelon (USVL531) v1

Overview


Name	CmUC07G130220
Type	gene
Organism	Citrullus mucosospermus (Watermelon (USVL531) v1)
Description	Cucumisin
Location	CmU531Chr07: 3808414 .. 3809187 (+)
RNA-Seq Expression	CmUC07G130220
Synteny	CmUC07G130220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATAGCTCTCCTACTGCAACCATTTTCAAGAGTACCACACTGCATAATGTGTCTGCTCCTGTTGTAATTTCCTTCTCATCCAGGGGACCTAATACTCTAACCAACGACATTCTCAAGGTTAAATTTATTTGAACTTTGCCTTTCTATATTGTTAGGTTAGGTAAGATGATTAATAAACTAATGTTATGAACTTGCACAAATGCAGCCAGATTTGAGTGGTCCAGGAGTTGAAATTTTAGCAGCATGGCCTCCTGTTGCGCCTGTTGCTAAAGTTCAAGATTCCAGGAGTACGCTTTGTAATATAATCTCAAGAATGTCAATGTCTAGCCCACATATCACTGGAATTGCAGTGTATGTTAAAACATTCAATCCTACGTGGTCTACTGCTGCCATCAAGTCAGCACTCATGACAACTGGTAATTATCACAGCCATGATATGACTAATTAACAAAGATTGTGGACTGCTCATCTTTCTAATATCCTTCTTTTGGACTTTCTATATCTCGTTCGCAAACCTTCAATCAATACTTCACAAGAACTCTCACAAGTGTTGCATCTTCAGCATCCACATATACAGCTATAATTTCTGCCCCACAAGGCTTTGAGATCACAGTGGATCCTAATATTCTATCATTCAATGGCATTGGAGATAGGAAATCTTTCACGTTGACTGTTCGAGGATCAGTGAGCCAGTTCATAGTCTCTGCTTCTTTGGTGTGGAGCGATGGTGTGCACACTGTGAGAAGCCCTATAACAATCACTTCTCGCTAG

mRNA sequence

ATGAATAGCTCTCCTACTGCAACCATTTTCAAGAGTACCACACTGCATAATGTGTCTGCTCCTGTTGTAATTTCCTTCTCATCCAGGGGACCTAATACTCTAACCAACGACATTCTCAAGCCAGATTTGAGTGGTCCAGGAGTTGAAATTTTAGCAGCATGGCCTCCTGTTGCGCCTGTTGCTAAAGTTCAAGATTCCAGGAGTACGCTTTGTAATATAATCTCAAGAATGTCAATGTCTAGCCCACATATCACTGGAATTGCAGTGTATGTTAAAACATTCAATCCTACGTGGTCTACTGCTGCCATCAAGTCAGCACTCATGACAACTGCATCCACATATACAGCTATAATTTCTGCCCCACAAGGCTTTGAGATCACAGTGGATCCTAATATTCTATCATTCAATGGCATTGGAGATAGGAAATCTTTCACGTTGACTGTTCGAGGATCAGTGAGCCAGTTCATAGTCTCTGCTTCTTTGGTGTGGAGCGATGGTGTGCACACTGTGAGAAGCCCTATAACAATCACTTCTCGCTAG

Coding sequence (CDS)

ATGAATAGCTCTCCTACTGCAACCATTTTCAAGAGTACCACACTGCATAATGTGTCTGCTCCTGTTGTAATTTCCTTCTCATCCAGGGGACCTAATACTCTAACCAACGACATTCTCAAGCCAGATTTGAGTGGTCCAGGAGTTGAAATTTTAGCAGCATGGCCTCCTGTTGCGCCTGTTGCTAAAGTTCAAGATTCCAGGAGTACGCTTTGTAATATAATCTCAAGAATGTCAATGTCTAGCCCACATATCACTGGAATTGCAGTGTATGTTAAAACATTCAATCCTACGTGGTCTACTGCTGCCATCAAGTCAGCACTCATGACAACTGCATCCACATATACAGCTATAATTTCTGCCCCACAAGGCTTTGAGATCACAGTGGATCCTAATATTCTATCATTCAATGGCATTGGAGATAGGAAATCTTTCACGTTGACTGTTCGAGGATCAGTGAGCCAGTTCATAGTCTCTGCTTCTTTGGTGTGGAGCGATGGTGTGCACACTGTGAGAAGCCCTATAACAATCACTTCTCGCTAG

Protein sequence

MNSSPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPVAPVAKVQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTTASTYTAIISAPQGFEITVDPNILSFNGIGDRKSFTLTVRGSVSQFIVSASLVWSDGVHTVRSPITITSR

Homology

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BLAST of CmUC07G130220 vs. NCBI nr
Match: XP_038892506.1 (cucumisin-like [Benincasa hispida])

HSP 1 Score: 221.5 bits (563), Expect = 5.9e-54
Identity = 136/280 (48.57%), Postives = 153/280 (54.64%), Query Frame = 0


Query: 3   SSPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPVAPVAK 62
           S PTATIFKST + N SAPVV+SFSSRGPN  T D++KPDLS PGVEILAAWPPVAPV  
Sbjct: 458 SFPTATIFKSTAILNTSAPVVVSFSSRGPNAATKDLIKPDLSAPGVEILAAWPPVAPVGG 517

Query: 63  VQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTT------------ 122
           +   R TL NIIS  SMS PH+TGIA YVKTFNPTWS AAIKSALMTT            
Sbjct: 518 I--LRDTLYNIISGTSMSCPHVTGIAAYVKTFNPTWSPAAIKSALMTTASPMNARFHPQG 577

Query: 123 ------------------------------------------------------------ 179
                                                                       
Sbjct: 578 EFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYSTSMVRRITGDYSACTSGNIGRV 637

[Close]

BLAST of CmUC07G130220 vs. NCBI nr
Match: KAA0051619.1 (cucumisin [Cucumis melo var. makuwa])

HSP 1 Score: 220.3 bits (560), Expect = 1.3e-53
Identity = 136/280 (48.57%), Postives = 154/280 (55.00%), Query Frame = 0


Query: 4   SPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPVAPVAKV 63
           SP ATIFKSTT+ N SAPVV+SFSSRGPN  T D++KPD+SGPGVEILAAWP VAPV  +
Sbjct: 404 SPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGI 463

Query: 64  QDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTT------------- 123
           +  R+TL NIIS  SMS PHITGIA YVKT+NPTWS AAIKSALMTT             
Sbjct: 464 R--RNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAE 523

Query: 124 ------------------------------------------------------------ 180
                                                                       
Sbjct: 524 FAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTEAVRRITGDYSACTPGNTGRVW 583

[Close]

BLAST of CmUC07G130220 vs. NCBI nr
Match: XP_008461721.1 (PREDICTED: LOW QUALITY PROTEIN: cucumisin [Cucumis melo])

HSP 1 Score: 219.2 bits (557), Expect = 2.9e-53
Identity = 136/279 (48.75%), Postives = 153/279 (54.84%), Query Frame = 0


Query: 4   SPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPVAPVAKV 63
           SP ATIFKSTT+ N SAPVV+SFSSRGPN  T D++KPD+SGPGVEILAAWP VAPV  +
Sbjct: 453 SPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGI 512

Query: 64  QDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTT------------- 123
           +  R+TL NIIS  SMS PHITGIA YVKT+NPTWS AAIKSALMTT             
Sbjct: 513 R--RNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAE 572

Query: 124 ------------------------------------------------------------ 179
                                                                       
Sbjct: 573 FAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTEAVRRITGDYSACTPGNTGRVW 632

[Close]

BLAST of CmUC07G130220 vs. NCBI nr
Match: TYK30793.1 (cucumisin [Cucumis melo var. makuwa])

HSP 1 Score: 218.0 bits (554), Expect = 6.6e-53
Identity = 136/286 (47.55%), Postives = 154/286 (53.85%), Query Frame = 0


Query: 4   SPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPVAPVAKV 63
           SP ATIFKSTT+ N SAPVV+SFSSRGPN  T D++KPD+SGPGVEILAAWP VAPV  +
Sbjct: 400 SPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGI 459

Query: 64  QDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTT------------- 123
           +  R+TL NIIS  SMS PHITGIA YVKT+NPTWS AAIKSALMTT             
Sbjct: 460 R--RNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTANYFTLTSPMNAR 519

Query: 124 ------------------------------------------------------------ 180
                                                                       
Sbjct: 520 FNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTEAVRRITGDYSACTPG 579

[Close]

BLAST of CmUC07G130220 vs. NCBI nr
Match: 3VTA_A (Crystal Structure of cucumisin, a subtilisin-like endoprotease from Cucumis melo L [Cucumis melo] >3VTA_B Crystal Structure of cucumisin, a subtilisin-like endoprotease from Cucumis melo L [Cucumis melo] >4YN3_A Crystal structure of Cucumisin complex with pro-peptide [Cucumis melo])

HSP 1 Score: 218.0 bits (554), Expect = 6.6e-53
Identity = 136/279 (48.75%), Postives = 152/279 (54.48%), Query Frame = 0


Query: 4   SPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPVAPVAKV 63
           SP ATIFKSTT+ N SAPVV+SFSSRGPN  T D++KPD+SGPGVEILAAWP VAPV  +
Sbjct: 343 SPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGI 402

Query: 64  QDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTT------------- 123
           +  R+TL NIIS  SMS PHITGIA YVKT+NPTWS AAIKSALMTT             
Sbjct: 403 R--RNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAE 462

Query: 124 ------------------------------------------------------------ 179
                                                                       
Sbjct: 463 FAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVW 522

[Close]

BLAST of CmUC07G130220 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 8.6e-56
Identity = 136/279 (48.75%), Postives = 152/279 (54.48%), Query Frame = 0


Query: 4   SPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPVAPVAKV 63
           SP ATIFKSTT+ N SAPVV+SFSSRGPN  T D++KPD+SGPGVEILAAWP VAPV  +
Sbjct: 453 SPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGI 512

Query: 64  QDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTT------------- 123
           +  R+TL NIIS  SMS PHITGIA YVKT+NPTWS AAIKSALMTT             
Sbjct: 513 R--RNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAE 572

Query: 124 ------------------------------------------------------------ 179
                                                                       
Sbjct: 573 FAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVW 632

[Close]

BLAST of CmUC07G130220 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 2.2e-27
Identity = 88/284 (30.99%), Postives = 125/284 (44.01%), Query Frame = 0


Query: 4   SPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPVAPVAKV 63
           SP A + K+  + N ++PV+ SFSSRGPNT+  DILKPD++ PGVEILAA+ P    ++ 
Sbjct: 445 SPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPSQ- 504

Query: 64  QDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTTA------------ 123
            D+R    +++S  SMS PH+ G+A YVKTFNP WS + I+SA+MTTA            
Sbjct: 505 DDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIAS 564

Query: 124 ------------------------------------------------------------ 177
                                                                       
Sbjct: 565 TEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKIL 624

[Close]

BLAST of CmUC07G130220 vs. ExPASy Swiss-Prot
Match: Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 3.2e-26
Identity = 88/282 (31.21%), Postives = 124/282 (43.97%), Query Frame = 0


Query: 4   SPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPVAPVAKV 63
           +P A + KS T+ N  APVV S+SSRGPN L +DILKPD++ PG EILAA+ P  P ++ 
Sbjct: 454 NPKAAVLKSETIFNQKAPVVASYSSRGPNPLIHDILKPDITAPGSEILAAYSPYVPPSE- 513

Query: 64  QDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTTA------------ 123
            D+R     +IS  SMS PH+ G+A Y+KTF+P WS + I+SA+MTTA            
Sbjct: 514 SDTRHVKYTVISGTSMSCPHVAGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNE 573

Query: 124 ------------------------------------------------------------ 177
                                                                       
Sbjct: 574 LAEFAYGAGHVDPIAAIHPGLVYEANKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTK 633

[Close]

BLAST of CmUC07G130220 vs. ExPASy Swiss-Prot
Match: Q9FIM5 (Subtilisin-like protease SBT4.9 OS=Arabidopsis thaliana OX=3702 GN=SBT4.9 PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 4.6e-25
Identity = 84/283 (29.68%), Postives = 122/283 (43.11%), Query Frame = 0


Query: 4   SPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPV-APVAK 63
           SP  T+ KS  + N +AP V  FSSRGPNT+  DILKPD++ PGVEILAA+ P+ +P   
Sbjct: 424 SPHGTVLKSEAIFNQAAPKVAGFSSRGPNTIAVDILKPDVTAPGVEILAAFSPLNSPAQD 483

Query: 64  VQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTTA----------- 123
            +D+R    +++S  SMS PH+ G+A Y+KTF+P WS + I+SA+MTTA           
Sbjct: 484 KRDNRHVKYSVLSGTSMSCPHVAGVAAYIKTFHPEWSPSMIQSAIMTTAWPMNATGTAVA 543

Query: 124 ------------------------------------------------------------ 177
                                                                       
Sbjct: 544 STEFAYGAGHVDPIAAINPGLVYEIGKSDHIAFLCGLNYNATSLKLIAGEAVTCTGKTLP 603

[Close]

BLAST of CmUC07G130220 vs. ExPASy Swiss-Prot
Match: Q9FIM6 (Subtilisin-like protease SBT4.8 OS=Arabidopsis thaliana OX=3702 GN=SBT4.8 PE=3 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 2.3e-24
Identity = 85/283 (30.04%), Postives = 120/283 (42.40%), Query Frame = 0


Query: 4   SPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPVA-PVAK 63
           SP  ++ K+  + N  +P V SFSSRGPNT+  DILKPD+S PGVEILAA+ P++ P   
Sbjct: 423 SPQGSVLKTEAIFNQLSPKVASFSSRGPNTIAVDILKPDISAPGVEILAAYSPLSLPSED 482

Query: 64  VQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTTA----------- 123
            +D R    +++S  SM+ PH+TG+A Y+KTF+P WS + I+SA+MTTA           
Sbjct: 483 RRDKRRVKYSVLSGTSMACPHVTGVAAYIKTFHPDWSPSVIQSAIMTTAWQMNATGTGAE 542

Query: 124 ------------------------------------------------------------ 177
                                                                       
Sbjct: 543 STEFAYGAGHVDPIAAINPGLVYELNKTDHISFLCGMNYTSKTLKLISGDAVICSGKTLQ 602

[Close]

BLAST of CmUC07G130220 vs. ExPASy TrEMBL
Match: A0A5A7UDR3 (Cucumisin OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G001700 PE=3 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 6.4e-54
Identity = 136/280 (48.57%), Postives = 154/280 (55.00%), Query Frame = 0


Query: 4   SPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPVAPVAKV 63
           SP ATIFKSTT+ N SAPVV+SFSSRGPN  T D++KPD+SGPGVEILAAWP VAPV  +
Sbjct: 404 SPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGI 463

Query: 64  QDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTT------------- 123
           +  R+TL NIIS  SMS PHITGIA YVKT+NPTWS AAIKSALMTT             
Sbjct: 464 R--RNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAE 523

Query: 124 ------------------------------------------------------------ 180
                                                                       
Sbjct: 524 FAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTEAVRRITGDYSACTPGNTGRVW 583

[Close]

BLAST of CmUC07G130220 vs. ExPASy TrEMBL
Match: A0A1S3CF86 (LOW QUALITY PROTEIN: cucumisin OS=Cucumis melo OX=3656 GN=LOC103500253 PE=3 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 1.4e-53
Identity = 136/279 (48.75%), Postives = 153/279 (54.84%), Query Frame = 0


Query: 4   SPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPVAPVAKV 63
           SP ATIFKSTT+ N SAPVV+SFSSRGPN  T D++KPD+SGPGVEILAAWP VAPV  +
Sbjct: 453 SPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGI 512

Query: 64  QDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTT------------- 123
           +  R+TL NIIS  SMS PHITGIA YVKT+NPTWS AAIKSALMTT             
Sbjct: 513 R--RNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAE 572

Query: 124 ------------------------------------------------------------ 179
                                                                       
Sbjct: 573 FAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTEAVRRITGDYSACTPGNTGRVW 632

[Close]

BLAST of CmUC07G130220 vs. ExPASy TrEMBL
Match: A0A5D3E3U6 (Cucumisin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold267G00160 PE=3 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 3.2e-53
Identity = 136/286 (47.55%), Postives = 154/286 (53.85%), Query Frame = 0


Query: 4   SPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPVAPVAKV 63
           SP ATIFKSTT+ N SAPVV+SFSSRGPN  T D++KPD+SGPGVEILAAWP VAPV  +
Sbjct: 400 SPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGI 459

Query: 64  QDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTT------------- 123
           +  R+TL NIIS  SMS PHITGIA YVKT+NPTWS AAIKSALMTT             
Sbjct: 460 R--RNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTANYFTLTSPMNAR 519

Query: 124 ------------------------------------------------------------ 180
                                                                       
Sbjct: 520 FNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTEAVRRITGDYSACTPG 579

[Close]

BLAST of CmUC07G130220 vs. ExPASy TrEMBL
Match: A0A6J1GZY5 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458774 PE=3 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 7.1e-53
Identity = 138/278 (49.64%), Postives = 150/278 (53.96%), Query Frame = 0


Query: 5   PTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPVAPVAKVQ 64
           PTATIFKS    + SAPVV+SFSSRGPN LTNDILKPDLSGPGVEILAAWPPVAP+A V+
Sbjct: 459 PTATIFKSVAGQDSSAPVVVSFSSRGPNALTNDILKPDLSGPGVEILAAWPPVAPIAGVK 518

Query: 65  DSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTTA------------- 124
           DSRS+L NIIS  SMS PH+TG+AVYVKTFNPT S AAIKSALMTTA             
Sbjct: 519 DSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALMTTASPMSARQHSQAEF 578

Query: 125 ------------------------------------------------------------ 179
                                                                       
Sbjct: 579 AYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYDTAMVRRITGDNSACTSGNIGRVWD 638

[Close]

BLAST of CmUC07G130220 vs. ExPASy TrEMBL
Match: A0A4P2YW59 (Pre-pro-cucumisin like serine protease OS=Trichosanthes bracteata OX=486425 PE=2 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 5.1e-51
Identity = 129/278 (46.40%), Postives = 150/278 (53.96%), Query Frame = 0


Query: 3   SSPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPVAPVAK 62
           S+PTA I   TT+ + SAPVVISFSSRGPN LTND+LKPD++GPGVEILAAWPP+AP++ 
Sbjct: 455 STPTARILSGTTVRDASAPVVISFSSRGPNALTNDLLKPDITGPGVEILAAWPPIAPISG 514

Query: 63  VQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTT------------ 122
           V++SRSTL NIIS  SMS PH++ +AVYVKTFNP+WS AAIKSALMTT            
Sbjct: 515 VRESRSTLFNIISGTSMSCPHLSAVAVYVKTFNPSWSPAAIKSALMTTASPMTARLNSEA 574

Query: 123 ------------------------------------------------------------ 177
                                                                       
Sbjct: 575 EFAYGSGHVNPLKAVNPGLVYDADQSDYVKFLCGQGYNTAMLRRITGDNSACTSANIGRV 634

[Close]

BLAST of CmUC07G130220 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 123.6 bits (309), Expect = 1.6e-28
Identity = 88/284 (30.99%), Postives = 125/284 (44.01%), Query Frame = 0


Query: 4   SPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPVAPVAKV 63
           SP A + K+  + N ++PV+ SFSSRGPNT+  DILKPD++ PGVEILAA+ P    ++ 
Sbjct: 445 SPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPSQ- 504

Query: 64  QDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTTA------------ 123
            D+R    +++S  SMS PH+ G+A YVKTFNP WS + I+SA+MTTA            
Sbjct: 505 DDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIAS 564

Query: 124 ------------------------------------------------------------ 177
                                                                       
Sbjct: 565 TEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKIL 624

[Close]

BLAST of CmUC07G130220 vs. TAIR 10
Match: AT3G46850.1 (Subtilase family protein )

HSP 1 Score: 119.8 bits (299), Expect = 2.3e-27
Identity = 88/282 (31.21%), Postives = 124/282 (43.97%), Query Frame = 0


Query: 4   SPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPVAPVAKV 63
           +P A + KS T+ N  APVV S+SSRGPN L +DILKPD++ PG EILAA+ P  P ++ 
Sbjct: 454 NPKAAVLKSETIFNQKAPVVASYSSRGPNPLIHDILKPDITAPGSEILAAYSPYVPPSE- 513

Query: 64  QDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTTA------------ 123
            D+R     +IS  SMS PH+ G+A Y+KTF+P WS + I+SA+MTTA            
Sbjct: 514 SDTRHVKYTVISGTSMSCPHVAGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNE 573

Query: 124 ------------------------------------------------------------ 177
                                                                       
Sbjct: 574 LAEFAYGAGHVDPIAAIHPGLVYEANKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTK 633

[Close]

BLAST of CmUC07G130220 vs. TAIR 10
Match: AT5G58840.1 (Subtilase family protein )

HSP 1 Score: 115.9 bits (289), Expect = 3.3e-26
Identity = 84/283 (29.68%), Postives = 122/283 (43.11%), Query Frame = 0


Query: 4   SPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPV-APVAK 63
           SP  T+ KS  + N +AP V  FSSRGPNT+  DILKPD++ PGVEILAA+ P+ +P   
Sbjct: 424 SPHGTVLKSEAIFNQAAPKVAGFSSRGPNTIAVDILKPDVTAPGVEILAAFSPLNSPAQD 483

Query: 64  VQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTTA----------- 123
            +D+R    +++S  SMS PH+ G+A Y+KTF+P WS + I+SA+MTTA           
Sbjct: 484 KRDNRHVKYSVLSGTSMSCPHVAGVAAYIKTFHPEWSPSMIQSAIMTTAWPMNATGTAVA 543

Query: 124 ------------------------------------------------------------ 177
                                                                       
Sbjct: 544 STEFAYGAGHVDPIAAINPGLVYEIGKSDHIAFLCGLNYNATSLKLIAGEAVTCTGKTLP 603

[Close]

BLAST of CmUC07G130220 vs. TAIR 10
Match: AT5G59090.2 (subtilase 4.12 )

HSP 1 Score: 115.2 bits (287), Expect = 5.6e-26
Identity = 86/279 (30.82%), Postives = 121/279 (43.37%), Query Frame = 0


Query: 4   SPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPVAPVAKV 63
           SP A + K+ T+ N ++PV+ SFSSRGPNT+  DILKPD++ PGVEILAA+ P    ++ 
Sbjct: 446 SPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPSE- 505

Query: 64  QDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTTA------------ 123
            D+R    ++ S  SM+ PH+ G+A YVKTF P WS + I+SA+MTTA            
Sbjct: 506 DDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAKGRGIASTEFAY 565

Query: 124 ------------------------------------------------------------ 177
                                                                       
Sbjct: 566 GAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLN 625

[Close]

BLAST of CmUC07G130220 vs. TAIR 10
Match: AT5G58830.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 113.6 bits (283), Expect = 1.6e-25
Identity = 85/283 (30.04%), Postives = 120/283 (42.40%), Query Frame = 0


Query: 4   SPTATIFKSTTLHNVSAPVVISFSSRGPNTLTNDILKPDLSGPGVEILAAWPPVA-PVAK 63
           SP  ++ K+  + N  +P V SFSSRGPNT+  DILKPD+S PGVEILAA+ P++ P   
Sbjct: 414 SPQGSVLKTEAIFNQLSPKVASFSSRGPNTIAVDILKPDISAPGVEILAAYSPLSLPSED 473

Query: 64  VQDSRSTLCNIISRMSMSSPHITGIAVYVKTFNPTWSTAAIKSALMTTA----------- 123
            +D R    +++S  SM+ PH+TG+A Y+KTF+P WS + I+SA+MTTA           
Sbjct: 474 RRDKRRVKYSVLSGTSMACPHVTGVAAYIKTFHPDWSPSVIQSAIMTTAWQMNATGTGAE 533

Query: 124 ------------------------------------------------------------ 177
                                                                       
Sbjct: 534 STEFAYGAGHVDPIAAINPGLVYELNKTDHISFLCGMNYTSKTLKLISGDAVICSGKTLQ 593

The following BLAST results are available for this feature:

BLAST of CmUC07G130220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038892506.1	5.9e-54	48.57	cucumisin-like [Benincasa hispida]	[more]
KAA0051619.1	1.3e-53	48.57	cucumisin [Cucumis melo var. makuwa]	[more]
XP_008461721.1	2.9e-53	48.75	PREDICTED: LOW QUALITY PROTEIN: cucumisin [Cucumis melo]	[more]
TYK30793.1	6.6e-53	47.55	cucumisin [Cucumis melo var. makuwa]	[more]
3VTA_A	6.6e-53	48.75	Crystal Structure of cucumisin, a subtilisin-like endoprotease from Cucumis melo...	[more]

BLAST of CmUC07G130220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39547	8.6e-56	48.75	Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1	[more]
Q9FIG2	2.2e-27	30.99	Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2...	[more]
Q9STF7	3.2e-26	31.21	Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 S...	[more]
Q9FIM5	4.6e-25	29.68	Subtilisin-like protease SBT4.9 OS=Arabidopsis thaliana OX=3702 GN=SBT4.9 PE=2 S...	[more]
Q9FIM6	2.3e-24	30.04	Subtilisin-like protease SBT4.8 OS=Arabidopsis thaliana OX=3702 GN=SBT4.8 PE=3 S...	[more]

BLAST of CmUC07G130220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UDR3	6.4e-54	48.57	Cucumisin OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G001700 PE=...	[more]
A0A1S3CF86	1.4e-53	48.75	LOW QUALITY PROTEIN: cucumisin OS=Cucumis melo OX=3656 GN=LOC103500253 PE=3 SV=1	[more]
A0A5D3E3U6	3.2e-53	47.55	Cucumisin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold267G00160 PE=3...	[more]
A0A6J1GZY5	7.1e-53	49.64	cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458774 PE=3 SV=1	[more]
A0A4P2YW59	5.1e-51	46.40	Pre-pro-cucumisin like serine protease OS=Trichosanthes bracteata OX=486425 PE=2...	[more]

BLAST of CmUC07G130220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59120.1	1.6e-28	30.99	subtilase 4.13	[more]
AT3G46850.1	2.3e-27	31.21	Subtilase family protein	[more]
AT5G58840.1	3.3e-26	29.68	Subtilase family protein	[more]
AT5G59090.2	5.6e-26	30.82	subtilase 4.12	[more]
AT5G58830.1	1.6e-25	30.04	Subtilisin-like serine endopeptidase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.50.30.30		coord: 3..16 e-value: 7.8E-40 score: 139.0
None	No IPR available	PANTHER	PTHR10795:SF685	SUBTILISIN-LIKE PROTEASE SBT4.3	coord: 3..116
None	No IPR available	PANTHER	PTHR10795:SF685	SUBTILISIN-LIKE PROTEASE SBT4.3	coord: 113..177
None	No IPR available	PROSITE	PS51892	SUBTILASE	coord: 1..134 score: 8.82906
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 22..115 e-value: 1.4E-12 score: 47.5
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 17..116 e-value: 7.8E-40 score: 139.0
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 15..116
IPR041469	Subtilisin-like protease, fibronectin type-III domain	PFAM	PF17766	fn3_6	coord: 109..176 e-value: 1.3E-16 score: 60.6
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 113..177 coord: 3..116

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmUC07G130220.1	CmUC07G130220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0008236	serine-type peptidase activity