CmUC06G113550 (gene) Watermelon (USVL531) v1

Overview
NameCmUC06G113550
Typegene
OrganismCitrullus mucosospermus (Watermelon (USVL531) v1)
DescriptionProcollagen-proline 4-dioxygenase
LocationCmU531Chr06: 6046470 .. 6051220 (-)
RNA-Seq ExpressionCmUC06G113550
SyntenyCmUC06G113550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GACTTCTGTGAGAGGAAAGAAAGCCTCTGCTCTGTGAAGTTCTGGATTTCCAATTTTCCAGACTTCCATCGAATTTCCTCCGATAATTCTCTCTCTCTCTCTCGCTCTCTTTCTAATTTGATCCGATCGAAACTATGTTTAAATTTCCTAATCTGTTATTCATCTGCTTGATTTTGATCTCATTGGTTGTTCGGGAATCAACTTGTTCGTATGCTGGTTCGGCTAGCTCCACTGTAGATCCTAGTAAAGTGAAGCAGATTTCATGGAAACCGAGGTTTTTATCGCTATCATTTTCATATGCACGGATCTTCTTTTTGTTTTCTTCTTCGTTCTATTGTGTTTGTTTGCTGAGAAAGTTGTGGAACGGAAGTTGAACTCTAAATTGCGTTAGCTTCTAATTTCCGGTCGTCTAGGTTTAGTTCAGTGACTGTTAATAGAAATTTTTGCTTTCCAAAATTTTGATAAGGCGATTTCACTGAATGTTGCAGAGCTTTTGTTTATGAAGGTTTTCTTACGGACCTAGAATGCGACCACCTGGTTTCTATTGTAAGTTTAGATTTATTGATTACTCGGTTTGATAATGGAAGTCATGTCATTGTCGTCAATATAATTCTCTGGTTGAAGGCTTGTTTTCTTTTGCGATAGGCAAGATCCGAGCTAAAAAGATCTGAGGTTGCTGATAACGATTCAGGAGAGAGCAAGCTCAGTACTGTTCGAACGAGTTCGGGAATGTTCATTTCTAAAAGCAAGGTTAGGTTTAATCTCCATACCAACAATCTTCCAATTTTTCTGGATGTTTTCTCTAATTCCTCTTTCGGTTTTTGTTTTTGATCGGAATCAAACTTCTGTTACTAGTCGTCACTCTAGCTATTTATTGAATTCCAACTAAATGGAAGAAACTTCTCTGTTGCGAAACTGAAATATGTTGGCTTCTCAAAGAGGTTAAACAATCAAATTACGACAGGAATGTTCCTGATAGCATGGATGTAATAATCAACAATAACATTATGTTGACTGTTAATACAAGATTAGAGAGAGAATAGTTTTTGAGGAATAATAACTTCAAACCTCCTTCTCTCCTATCTTGTTGAATTAGGACCAGATTAAATAGTTTGAGCGGTTAGGTTTTAATAAATCTAACATACAGTGTACTTTGTGCTCTTCCCCTGTCTCTTAGAATTGATGTATATGGTATGACGATAACTATTTCCTTTGGTTCTCAAGTATCAGTTGAGATCTCTTCTCTTTACTCCCACGTTTCTGTCAAATATTTACATTTTTACTTGGTATTTAGGATCCTATTGTTTCTGGCATAGAGGACAAAATTGCTGCATGGACTTTTCTTCCAAAAGGTACACGATTTGGTTCACTGTCAAATCACATATTTTGTCATTGATATGTTATGTTATATGCATTTTAATTACTACAGTTAATTTCCATAAGTAACATTCATGGTTTCTTTATAATTATACCTGGGAGAAGCCAAGGAGTGCATATATTATTGAAATGGTTGTCAATGTCAAGTATCACTGCTAAATGTTGAGGGTATTTCTCGTTTTTCAGAAAATGGGGAGGATATTCAGGTATTGAGATATGAGCATGGGCAGAAATATGAGTCACATTATGATTACTTTGTTGACAAGGTGAATATTGCCTGGGGAGGACATCGTTTAGCTACAGTCCTCATGTATCTCTCTGATGTGACCAAAGGCGGTGAAACAGTTTTCCCCTTGGCAGAGGTTAGTTGAAGCGTTAATTCACAAACTACTTTGCTAGAACTGCACTTCATATTCCTCACTGTAAGATTACCTTAACTCTTCTTAGATCTCAGTAGTCAGTACTCTCTAAATAATAATCATACAACTCTGTCTTCTGATGGAAAGGGTCCTTTTATAAGACACTATACGCTTAAATATTGAAATGATACTCTGTCTTTTGATGGAAAGGATACTTCCATATGACTTTGATACATTGAAACATTGAAATGAAACATGAGAAAGACCCGATGTTGGATAATCTGAAGGAATTTGCCATATTTTATAGGAATTTCTACTGAAAATGACCTAATTTTTGTTCAGGTTGATGAAACTGAACCCTTTTGCCCAATTTGAACCATTTTTATTTGTTTGTTTTTCTTTTTGTTTTAATTTTCTTTTCCAGATTTTGTTCTCAATCAAAATGTCTATCAACTAATCCATATTCATTGGGGATGCAATACAATCCCGCTTGAAGGCAACTATCATGGGTTGGCCTAGTGGTAGTGGGAACATAAAAAAAAAGGCTAAAGGGCCAAAGAGTCATGGGTTCAATCCATGGTGGCCACCTATTTAGGATTTAATATCTTATGGGTTTCCTTGACACCCAAATGTTGTAAGGTCAGGCGGGTTGTGCTTGGAGGCATTTAGCACTTTCTGCTGCACCGTCGTCTTCTTAAGTACTCCAGTTAGGAAAAATGTTACTTCCTGCTGCACCGTCTTCTTAAGTACTCCAGTTAGGAAAAATGTTATTGTAGTTATTTCAAAGGACTGAAATTGGTTATAAAGTTACATATATATTAGAAATGAATTTGGTTGAGAAATGACTAAAATCGTTAGCAGACTTGTTTGACCTTGTTATATACCCTCTGAGCAGAAATCTCGCCACCGGAGGGCTGCTGAAACAGATGAGGATCTCTCAGAGTGTGCAAGGAAAGGAATTGCAGGTCAGTCGCCAAACAAACAACTATAAATGCTTCTTTTTCAGTACTTACCAAACTATAGAACTCGCATGAAATTGATGTGCTTTATTATTTGACATTATTCCCACTTGGAACGATGCAGTGAAACCGAAGAAAGGCGACGCCCTTCTTTTCTTTAGTCTTGAACCAAATGCTATCCCAGACACAAACAGTCTCCATGGAGGTTGCCCTGTCCTTGAAGGAGAAAAATGGTCAGCAACAAAGTGGATTCACGTAGACTCTTTCAGCAAAAACTTAGGAAACATTGGGAATTGTACTGATCTAAATGAAAGTTGTGAGAGATGGGCTGCCTTAGGAGAATGCACCAAAAACCCAGAATATATGGTCGGATCTCCAGAAATGCCTGGCTACTGTAGGCGGAGCTGCAGGATCTGTTGATCTCAACAATACACTCGAAATTTCCATACCTTTGGGCGGTAAAATTCTCAGTCATGGTTGGATAGTAATTGACTTGCTTTTAAATTATTGGCTTGGTTTGGAAATTTCTTTCCCTTTGAGTAGTACTTCGATTTGTTTAGGCTTTCCTATAAAAGTATTGTTTCTTCTAAAAAGAAGTAGGTTAAAATACAAATTTAGTCCGGCTCTTTTTAAATATAGCAAAATGAACTAAAATATTTACAAATATAACAAAGTTTCACTATCTATTTAATATAGACCATGATAGACTACTATCTATGTCACGATAGACACAGATAGTAGTCTATCGCGATCTATCGCAGATAGACGGTGTATTTTGCTTTTAGTTGTGAATATTTTCAGCAGTTTTGCCATTTAAGATAATCTTCCAATTTAGTCTCTATGATTTGAATCTTGTCTTTGGTGGATACTAATTGACTTATTTTTAAATTATTAGCTTAGTTTGGAAATTTCCACTTTGAATTGTTTAGGCTTTCCCATACTGAATGTATTGTTTCACCTAATAGAAATAGGTGAAGTTGCAAATTTGGTCCTTATGATTTGAAGAAAGTTAGAATTTAGTCCGTATGGTTTATAATTAGAATTTTGTCCTTCTGGTCAATAAAATCCTCCTAAATTGTCCCTACTAAGGACTAATTATTGGAATTTTATCAAATCATATAGGCTAAATTCTAATTTTGAGAACTATAGGGCCTAAATTCTAAATTTCTCCGACATAATTACTTGATAAAGTTTTCTCATTCATGAATTTCTTTATTTTTATTTATTTATTTATTTATTTTTTTGTGTCATCTGGAACGTCAATCATTTGTGGAATGAAGAGCACTACAACATTGATTGCGTAGCTATCTCTTGAAGCAAGGATGGATGAGGCAGTAGCCGTTGAGTAATTATGACCTTTCATTTTTAATTGATGAGTATGTATTACTTTATTCTCTTTATTTTTCTTATTTGATTTTTGATATGGTTTTCTTAGAAGAAAGAATATTGTAGTTTTAAAGGCTATAGTTTTATATATACATTACAGAAAAATTGAGTAATTTTGCTGATGTGACATTAGAGAGTCTATAACCTTTCATTTCAACAGAAATGTCTGGTACAAATGAGTTTTATAATACGAAGAGATAACAATACACTTGATCTATTTTAATTTTTAAATACTTTTTCTATGTCGAAAATAATTTGGTTTATGCATCTTTGAAGAGATGGGTTCATAAATGGGTCAACAATGAGCTCATTTGCAAGGAAGTCAATCGACTCTTTCTCTCCCCCTCAAATCTAGAAAACTTCTTTACCTTTTTTTCTTAATATGATTATCATTTTAATCTTTGTATTTTGGGATTTACTTATTTTAGTCCTTAATATTAATATTCATTTTTGTCAAATATTTCCAAAAAAAACCCTTATCAACCTTTCTTCTCCATTTTTCAAAATTAATTAATTAATTAAAATACTATTTTGGTCTAATTACTCTAAGATTAGTTTAAGATTAGTTCCATTTAAGTTGGTTCTTTCAAATTTCCAATGTTAGTACTTATACTTTTAATAAAACTTAAATATTAGCATTTTTACTACGATCTAGGAAATATATCCACAT

mRNA sequence

GACTTCTGTGAGAGGAAAGAAAGCCTCTGCTCTGTGAAGTTCTGGATTTCCAATTTTCCAGACTTCCATCGAATTTCCTCCGATAATTCTCTCTCTCTCTCTCGCTCTCTTTCTAATTTGATCCGATCGAAACTATGTTTAAATTTCCTAATCTGTTATTCATCTGCTTGATTTTGATCTCATTGGTTGTTCGGGAATCAACTTGTTCGTATGCTGGTTCGGCTAGCTCCACTGTAGATCCTAGTAAAGTGAAGCAGATTTCATGGAAACCGAGAGCTTTTGTTTATGAAGGTTTTCTTACGGACCTAGAATGCGACCACCTGGTTTCTATTGCAAGATCCGAGCTAAAAAGATCTGAGGTTGCTGATAACGATTCAGGAGAGAGCAAGCTCAGTACTGTTCGAACGAGTTCGGGAATGTTCATTTCTAAAAGCAAGGATCCTATTGTTTCTGGCATAGAGGACAAAATTGCTGCATGGACTTTTCTTCCAAAAGAAAATGGGGAGGATATTCAGGTATTGAGATATGAGCATGGGCAGAAATATGAGTCACATTATGATTACTTTGTTGACAAGGTGAATATTGCCTGGGGAGGACATCGTTTAGCTACAGTCCTCATGTATCTCTCTGATGTGACCAAAGGCGGTGAAACAGTTTTCCCCTTGGCAGAGAAATCTCGCCACCGGAGGGCTGCTGAAACAGATGAGGATCTCTCAGAGTGTGCAAGGAAAGGAATTGCAGTGAAACCGAAGAAAGGCGACGCCCTTCTTTTCTTTAGTCTTGAACCAAATGCTATCCCAGACACAAACAGTCTCCATGGAGGTTGCCCTGTCCTTGAAGGAGAAAAATGGTCAGCAACAAAGTGGATTCACGTAGACTCTTTCAGCAAAAACTTAGGAAACATTGGGAATTGTACTGATCTAAATGAAAGTTGTGAGAGATGGGCTGCCTTAGGAGAATGCACCAAAAACCCAGAATATATGGTCGGATCTCCAGAAATGCCTGGCTACTGTAGGCGGAGCTGCAGGATCTGTTGATCTCAACAATACACTCGAAATTTCCATACCTTTGGGCGAGCACTACAACATTGATTGCGTAGCTATCTCTTGAAGCAAGGATGGATGAGGCAGTAGCCGTTGAGTAATTATGACCTTTCATTTTTAATTGATGAGTATGTATTACTTTATTCTCTTTATTTTTCTTATTTGATTTTTGATATGGTTTTCTTAGAAGAAAGAATATTGTAGTTTTAAAGGCTATAGTTTTATATATACATTACAGAAAAATTGAGTAATTTTGCTGATGTGACATTAGAGAGTCTATAACCTTTCATTTCAACAGAAATGTCTGGTACAAATGAGTTTTATAATACGAAGAGATAACAATACACTTGATCTATTTTAATTTTTAAATACTTTTTCTATGTCGAAAATAATTTGGTTTATGCATCTTTGAAGAGATGGGTTCATAAATGGGTCAACAATGAGCTCATTTGCAAGGAAGTCAATCGACTCTTTCTCTCCCCCTCAAATCTAGAAAACTTCTTTACCTTTTTTTCTTAATATGATTATCATTTTAATCTTTGTATTTTGGGATTTACTTATTTTAGTCCTTAATATTAATATTCATTTTTGTCAAATATTTCCAAAAAAAACCCTTATCAACCTTTCTTCTCCATTTTTCAAAATTAATTAATTAATTAAAATACTATTTTGGTCTAATTACTCTAAGATTAGTTTAAGATTAGTTCCATTTAAGTTGGTTCTTTCAAATTTCCAATGTTAGTACTTATACTTTTAATAAAACTTAAATATTAGCATTTTTACTACGATCTAGGAAATATATCCACAT

Coding sequence (CDS)

ATGTTTAAATTTCCTAATCTGTTATTCATCTGCTTGATTTTGATCTCATTGGTTGTTCGGGAATCAACTTGTTCGTATGCTGGTTCGGCTAGCTCCACTGTAGATCCTAGTAAAGTGAAGCAGATTTCATGGAAACCGAGAGCTTTTGTTTATGAAGGTTTTCTTACGGACCTAGAATGCGACCACCTGGTTTCTATTGCAAGATCCGAGCTAAAAAGATCTGAGGTTGCTGATAACGATTCAGGAGAGAGCAAGCTCAGTACTGTTCGAACGAGTTCGGGAATGTTCATTTCTAAAAGCAAGGATCCTATTGTTTCTGGCATAGAGGACAAAATTGCTGCATGGACTTTTCTTCCAAAAGAAAATGGGGAGGATATTCAGGTATTGAGATATGAGCATGGGCAGAAATATGAGTCACATTATGATTACTTTGTTGACAAGGTGAATATTGCCTGGGGAGGACATCGTTTAGCTACAGTCCTCATGTATCTCTCTGATGTGACCAAAGGCGGTGAAACAGTTTTCCCCTTGGCAGAGAAATCTCGCCACCGGAGGGCTGCTGAAACAGATGAGGATCTCTCAGAGTGTGCAAGGAAAGGAATTGCAGTGAAACCGAAGAAAGGCGACGCCCTTCTTTTCTTTAGTCTTGAACCAAATGCTATCCCAGACACAAACAGTCTCCATGGAGGTTGCCCTGTCCTTGAAGGAGAAAAATGGTCAGCAACAAAGTGGATTCACGTAGACTCTTTCAGCAAAAACTTAGGAAACATTGGGAATTGTACTGATCTAAATGAAAGTTGTGAGAGATGGGCTGCCTTAGGAGAATGCACCAAAAACCCAGAATATATGGTCGGATCTCCAGAAATGCCTGGCTACTGTAGGCGGAGCTGCAGGATCTGTTGA

Protein sequence

MFKFPNLLFICLILISLVVRESTCSYAGSASSTVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSIARSELKRSEVADNDSGESKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPLAEKSRHRRAAETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIHVDSFSKNLGNIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC
Homology
BLAST of CmUC06G113550 vs. NCBI nr
Match: XP_038874583.1 (probable prolyl 4-hydroxylase 4 [Benincasa hispida])

HSP 1 Score: 602.1 bits (1551), Expect = 2.7e-168
Identity = 292/300 (97.33%), Postives = 296/300 (98.67%), Query Frame = 0

Query: 1   MFKFPNLLFICLILISLVVRESTCSYAGSASSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           MFKF NLLFI LILISLVVRESTCSYAGSASSTVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MFKFRNLLFIFLILISLVVRESTCSYAGSASSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  DHLVSIARSELKRSEVADNDSGESKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK 120
           DHLVSIARSELKRSEVADNDSG+SKLSTVRTSSGMFISKSKDPIVSGIEDKI+AWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKSKDPIVSGIEDKISAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPLAEK 180
           ENGEDIQVLRYEHG+KYESHYDYFVDKVNIAWGGHRLATVLMYLS+VTKGGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGEKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTKGGETVFPLAEK 180

Query: 181 SRHRRAAETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
           S HRRA ETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS
Sbjct: 181 SAHRRAYETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFSKNLGNIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSFSKNLGNIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC
Sbjct: 241 ATKWIHVDSFSKNLGNIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300

BLAST of CmUC06G113550 vs. NCBI nr
Match: XP_008458517.1 (PREDICTED: probable prolyl 4-hydroxylase 4 [Cucumis melo] >XP_008458518.1 PREDICTED: probable prolyl 4-hydroxylase 4 [Cucumis melo] >KAA0033447.1 putative prolyl 4-hydroxylase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 590.9 bits (1522), Expect = 6.2e-165
Identity = 285/300 (95.00%), Postives = 293/300 (97.67%), Query Frame = 0

Query: 1   MFKFPNLLFICLILISLVVRESTCSYAGSASSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           MFKF NLLF  LILIS  VRESTCSYAGSAS+TVDPS+VKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MFKFRNLLFFFLILISSFVRESTCSYAGSASATVDPSRVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  DHLVSIARSELKRSEVADNDSGESKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK 120
           DHLVSIARSELKRSEVADNDSG+SKLSTVRTSSGMFISK+KDPIVSGIEDKI+AWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLS+VTKGGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTKGGETVFPLAEK 180

Query: 181 SRHRRAAETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
           S HRRA ETDEDLSECA+KGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS
Sbjct: 181 SSHRRAYETDEDLSECAKKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFSKNLGNIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSFSKNLG+IGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC
Sbjct: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300

BLAST of CmUC06G113550 vs. NCBI nr
Match: XP_004144967.1 (probable prolyl 4-hydroxylase 4 [Cucumis sativus] >XP_011656650.1 probable prolyl 4-hydroxylase 4 [Cucumis sativus] >KGN46177.1 hypothetical protein Csa_004844 [Cucumis sativus])

HSP 1 Score: 587.8 bits (1514), Expect = 5.3e-164
Identity = 282/300 (94.00%), Postives = 292/300 (97.33%), Query Frame = 0

Query: 1   MFKFPNLLFICLILISLVVRESTCSYAGSASSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           MFKF NLLFI LIL S  +RESTCSYAGSAS+TVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  DHLVSIARSELKRSEVADNDSGESKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK 120
           DHLVSIARSELKRSEVADNDSG+SKLSTVRTSSGMFISK+KDPIVSGIEDKI+AWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLS+VT+GGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK 180

Query: 181 SRHRRAAETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
             HRRA ETDEDLSECA+KG+AVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS
Sbjct: 181 PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFSKNLGNIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSFSKNLG+IGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC
Sbjct: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300

BLAST of CmUC06G113550 vs. NCBI nr
Match: KAG6575033.1 (putative prolyl 4-hydroxylase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 582.4 bits (1500), Expect = 2.2e-162
Identity = 283/300 (94.33%), Postives = 293/300 (97.67%), Query Frame = 0

Query: 1   MFKFPNLLFICLILISLVVRESTCSYAGSASSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           M KF NLLF+ LILIS VVRES+CSYAGSA+STVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MSKFRNLLFLFLILISSVVRESSCSYAGSATSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  DHLVSIARSELKRSEVADNDSGESKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK 120
           DHLVSIARSELKRSEVADNDSG+SKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGDSKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIA GGHRLATVLMYLS+VTKGGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSNVTKGGETVFPLAEK 180

Query: 181 SRHRRAAETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
           S  RRA+ETDEDL+ECAR+GIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS
Sbjct: 181 SPRRRASETDEDLTECARQGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFSKNLGNIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSFSKNLGNIG+CTDLNESCERWAALGECTKNPEYMVGSPE+PGYCRRSCRIC
Sbjct: 241 ATKWIHVDSFSKNLGNIGDCTDLNESCERWAALGECTKNPEYMVGSPELPGYCRRSCRIC 300

BLAST of CmUC06G113550 vs. NCBI nr
Match: XP_022959148.1 (probable prolyl 4-hydroxylase 4 [Cucurbita moschata] >XP_022959149.1 probable prolyl 4-hydroxylase 4 [Cucurbita moschata])

HSP 1 Score: 582.0 bits (1499), Expect = 2.9e-162
Identity = 283/300 (94.33%), Postives = 293/300 (97.67%), Query Frame = 0

Query: 1   MFKFPNLLFICLILISLVVRESTCSYAGSASSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           M KF NLLF+ LILIS VVRES+CSYAGSA+STVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MSKFRNLLFLFLILISSVVRESSCSYAGSATSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  DHLVSIARSELKRSEVADNDSGESKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK 120
           DHLVSIARSELKRSEVADNDSG+SKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGDSKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIA GGHRLATVLMYLS+VTKGGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSNVTKGGETVFPLAEK 180

Query: 181 SRHRRAAETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
           S  RRA+ETDEDLSECAR+GIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS
Sbjct: 181 SPRRRASETDEDLSECARQGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFSKNLGNIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSFSKNLGNIG+C+DLNESCERWAALGECTKNPEYMVGSPE+PGYCRRSCRIC
Sbjct: 241 ATKWIHVDSFSKNLGNIGDCSDLNESCERWAALGECTKNPEYMVGSPELPGYCRRSCRIC 300

BLAST of CmUC06G113550 vs. ExPASy Swiss-Prot
Match: Q8LAN3 (Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 1.2e-126
Identity = 216/293 (73.72%), Postives = 248/293 (84.64%), Query Frame = 0

Query: 8   LFICLILISLVVRESTCSYAGSASSTVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSIA 67
           L I    I  V+ +S+ S   S+S  V+PSKVKQ+S KPRAFVYEGFLT+LECDH+VS+A
Sbjct: 6   LLISFFAIFSVLLQSSTSLISSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSLA 65

Query: 68  RSELKRSEVADNDSGESKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPKENGEDIQ 127
           ++ LKRS VADNDSGESK S VRTSSG FISK KDPIVSGIEDKI+ WTFLPKENGEDIQ
Sbjct: 66  KASLKRSAVADNDSGESKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDIQ 125

Query: 128 VLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPLAEKSRHRRAA 187
           VLRYEHGQKY++H+DYF DKVNI  GGHR+AT+LMYLS+VTKGGETVFP AE    R  +
Sbjct: 126 VLRYEHGQKYDAHFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVLS 185

Query: 188 ETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIHV 247
           E  EDLS+CA++GIAVKP+KGDALLFF+L P+AIPD  SLHGGCPV+EGEKWSATKWIHV
Sbjct: 186 ENKEDLSDCAKRGIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIHV 245

Query: 248 DSFSKNLGNIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           DSF + +   GNCTD+NESCERWA LGECTKNPEYMVG+ E+PGYCRRSC+ C
Sbjct: 246 DSFDRIVTPSGNCTDMNESCERWAVLGECTKNPEYMVGTTELPGYCRRSCKAC 298

BLAST of CmUC06G113550 vs. ExPASy Swiss-Prot
Match: F4JAU3 (Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 7.8e-126
Identity = 213/294 (72.45%), Postives = 255/294 (86.73%), Query Frame = 0

Query: 7   LLFICLILISLVVRESTCSYAGSASSTVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSI 66
           LLF+ ++L+  +++ STC    S SS ++PSKVKQ+S KPRAFVYEGFLTDLECDHL+S+
Sbjct: 9   LLFVAILLV--LLQSSTC-LISSPSSIINPSKVKQVSSKPRAFVYEGFLTDLECDHLISL 68

Query: 67  ARSELKRSEVADNDSGESKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPKENGEDI 126
           A+  L+RS VADND+GES++S VRTSSG FISK KDPIVSGIEDK++ WTFLPKENGED+
Sbjct: 69  AKENLQRSAVADNDNGESQVSDVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDL 128

Query: 127 QVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPLAEKSRHRRA 186
           QVLRYEHGQKY++H+DYF DKVNIA GGHR+ATVL+YLS+VTKGGETVFP A++   R  
Sbjct: 129 QVLRYEHGQKYDAHFDYFHDKVNIARGGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSL 188

Query: 187 AETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIH 246
           +E  +DLS+CA+KGIAVKPKKG+ALLFF+L+ +AIPD  SLHGGCPV+EGEKWSATKWIH
Sbjct: 189 SENKDDLSDCAKKGIAVKPKKGNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIH 248

Query: 247 VDSFSKNLGNIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           VDSF K L + GNCTD+NESCERWA LGEC KNPEYMVG+PE+PG CRRSC+ C
Sbjct: 249 VDSFDKILTHDGNCTDVNESCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

BLAST of CmUC06G113550 vs. ExPASy Swiss-Prot
Match: Q8L970 (Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 2.1e-99
Identity = 166/272 (61.03%), Postives = 209/272 (76.84%), Query Frame = 0

Query: 29  SASSTVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSIARSELKRSEVADNDSGESKLST 88
           ++S   DP++V Q+SW PR F+YEGFL+D ECDH + +A+ +L++S VADNDSGES  S 
Sbjct: 46  ASSFGFDPTRVTQLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESE 105

Query: 89  VRTSSGMFISKSKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEHGQKYESHYDYFVDKV 148
           VRTSSGMF+SK +D IVS +E K+AAWTFLP+ENGE +Q+L YE+GQKYE H+DYF D+ 
Sbjct: 106 VRTSSGMFLSKRQDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQA 165

Query: 149 NIAWGGHRLATVLMYLSDVTKGGETVFPLAEKSRHRRAAETDEDLSECARKGIAVKPKKG 208
           N+  GGHR+ATVLMYLS+V KGGETVFP+    + +     D+  +ECA++G AVKP+KG
Sbjct: 166 NLELGGHRIATVLMYLSNVEKGGETVFPM---WKGKATQLKDDSWTECAKQGYAVKPRKG 225

Query: 209 DALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIHVDSFSKNLGNIGNCTDLNESCE 268
           DALLFF+L PNA  D+NSLHG CPV+EGEKWSAT+WIHV SF +       C D N SCE
Sbjct: 226 DALLFFNLHPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSGCMDENVSCE 285

Query: 269 RWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           +WA  GEC KNP YMVGS +  GYCR+SC+ C
Sbjct: 286 KWAKAGECQKNPTYMVGSDKDHGYCRKSCKAC 314

BLAST of CmUC06G113550 vs. ExPASy Swiss-Prot
Match: F4J0A8 (Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 1.5e-92
Identity = 163/277 (58.84%), Postives = 208/277 (75.09%), Query Frame = 0

Query: 25  SYAGSASSTVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSIARSELKRS-EVADNDSGE 84
           S   S S +VDP+++ Q+SW PRAF+Y+GFL+D ECDHL+ +A+ +L++S  VAD DSGE
Sbjct: 18  SQISSFSFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGE 77

Query: 85  SKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEHGQKYESHYDY 144
           S+ S VRTSSGMF++K +D IV+ +E K+AAWTFLP+ENGE +Q+L YE+GQKY+ H+DY
Sbjct: 78  SEDSEVRTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDY 137

Query: 145 FVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPLAEKSRHRRAAETDEDLSECARKGIAV 204
           F DK  +  GGHR+ATVLMYLS+VTKGGETVFP     + +     D+  S+CA++G AV
Sbjct: 138 FYDKKALELGGHRIATVLMYLSNVTKGGETVFP---NWKGKTPQLKDDSWSKCAKQGYAV 197

Query: 205 KPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIHVDSFSKNLGNIGNCTDL 264
           KP+KGDALLFF+L  N   D NSLHG CPV+EGEKWSAT+WIHV SF K       C D 
Sbjct: 198 KPRKGDALLFFNLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHVRSFGKKK---LVCVDD 257

Query: 265 NESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           +ESC+ WA  GEC KNP YMVGS    G+CR+SC+ C
Sbjct: 258 HESCQEWADAGECEKNPMYMVGSETSLGFCRKSCKAC 288

BLAST of CmUC06G113550 vs. ExPASy Swiss-Prot
Match: Q9LN20 (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.3e-64
Identity = 114/209 (54.55%), Postives = 157/209 (75.12%), Query Frame = 0

Query: 42  ISWKPRAFVYEGFLTDLECDHLVSIARSELKRSEVADNDSGESKLSTVRTSSGMFISKSK 101
           +SW+PRAFVY  FL+  EC++L+S+A+  + +S V D+++G+SK S VRTSSG F+ + +
Sbjct: 79  LSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETGKSKDSRVRTSSGTFLRRGR 138

Query: 102 DPIVSGIEDKIAAWTFLPKENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVL 161
           D I+  IE +IA +TF+P ++GE +QVL YE GQKYE HYDYFVD+ N   GG R+AT+L
Sbjct: 139 DKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYDYFVDEFNTKNGGQRMATML 198

Query: 162 MYLSDVTKGGETVFPLAEKSRHRRAAETDEDLSECARKGIAVKPKKGDALLFFSLEPNAI 221
           MYLSDV +GGETVFP A  + +  +     +LSEC +KG++VKP+ GDALLF+S+ P+A 
Sbjct: 199 MYLSDVEEGGETVFPAA--NMNFSSVPWYNELSECGKKGLSVKPRMGDALLFWSMRPDAT 258

Query: 222 PDTNSLHGGCPVLEGEKWSATKWIHVDSF 251
            D  SLHGGCPV+ G KWS+TKW+HV  +
Sbjct: 259 LDPTSLHGGCPVIRGNKWSSTKWMHVGEY 285

BLAST of CmUC06G113550 vs. ExPASy TrEMBL
Match: A0A1S3C816 (Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103497901 PE=3 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 3.0e-165
Identity = 285/300 (95.00%), Postives = 293/300 (97.67%), Query Frame = 0

Query: 1   MFKFPNLLFICLILISLVVRESTCSYAGSASSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           MFKF NLLF  LILIS  VRESTCSYAGSAS+TVDPS+VKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MFKFRNLLFFFLILISSFVRESTCSYAGSASATVDPSRVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  DHLVSIARSELKRSEVADNDSGESKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK 120
           DHLVSIARSELKRSEVADNDSG+SKLSTVRTSSGMFISK+KDPIVSGIEDKI+AWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLS+VTKGGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTKGGETVFPLAEK 180

Query: 181 SRHRRAAETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
           S HRRA ETDEDLSECA+KGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS
Sbjct: 181 SSHRRAYETDEDLSECAKKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFSKNLGNIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSFSKNLG+IGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC
Sbjct: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300

BLAST of CmUC06G113550 vs. ExPASy TrEMBL
Match: A0A5A7SVW6 (Procollagen-proline 4-dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G001080 PE=3 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 3.0e-165
Identity = 285/300 (95.00%), Postives = 293/300 (97.67%), Query Frame = 0

Query: 1   MFKFPNLLFICLILISLVVRESTCSYAGSASSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           MFKF NLLF  LILIS  VRESTCSYAGSAS+TVDPS+VKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MFKFRNLLFFFLILISSFVRESTCSYAGSASATVDPSRVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  DHLVSIARSELKRSEVADNDSGESKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK 120
           DHLVSIARSELKRSEVADNDSG+SKLSTVRTSSGMFISK+KDPIVSGIEDKI+AWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLS+VTKGGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTKGGETVFPLAEK 180

Query: 181 SRHRRAAETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
           S HRRA ETDEDLSECA+KGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS
Sbjct: 181 SSHRRAYETDEDLSECAKKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFSKNLGNIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSFSKNLG+IGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC
Sbjct: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300

BLAST of CmUC06G113550 vs. ExPASy TrEMBL
Match: A0A0A0KCQ5 (Procollagen-proline 4-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_6G067350 PE=3 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 2.6e-164
Identity = 282/300 (94.00%), Postives = 292/300 (97.33%), Query Frame = 0

Query: 1   MFKFPNLLFICLILISLVVRESTCSYAGSASSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           MFKF NLLFI LIL S  +RESTCSYAGSAS+TVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MFKFDNLLFIFLILTSSFIRESTCSYAGSASATVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  DHLVSIARSELKRSEVADNDSGESKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK 120
           DHLVSIARSELKRSEVADNDSG+SKLSTVRTSSGMFISK+KDPIVSGIEDKI+AWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGKSKLSTVRTSSGMFISKNKDPIVSGIEDKISAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLS+VT+GGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSNVTQGGETVFPLAEK 180

Query: 181 SRHRRAAETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
             HRRA ETDEDLSECA+KG+AVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS
Sbjct: 181 PSHRRAYETDEDLSECAKKGVAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFSKNLGNIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSFSKNLG+IGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC
Sbjct: 241 ATKWIHVDSFSKNLGDIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300

BLAST of CmUC06G113550 vs. ExPASy TrEMBL
Match: A0A6J1H545 (Procollagen-proline 4-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111460227 PE=3 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 1.4e-162
Identity = 283/300 (94.33%), Postives = 293/300 (97.67%), Query Frame = 0

Query: 1   MFKFPNLLFICLILISLVVRESTCSYAGSASSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           M KF NLLF+ LILIS VVRES+CSYAGSA+STVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MSKFRNLLFLFLILISSVVRESSCSYAGSATSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  DHLVSIARSELKRSEVADNDSGESKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK 120
           DHLVSIARSELKRSEVADNDSG+SKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGDSKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIA GGHRLATVLMYLS+VTKGGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSNVTKGGETVFPLAEK 180

Query: 181 SRHRRAAETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
           S  RRA+ETDEDLSECAR+GIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS
Sbjct: 181 SPRRRASETDEDLSECARQGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFSKNLGNIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSFSKNLGNIG+C+DLNESCERWAALGECTKNPEYMVGSPE+PGYCRRSCRIC
Sbjct: 241 ATKWIHVDSFSKNLGNIGDCSDLNESCERWAALGECTKNPEYMVGSPELPGYCRRSCRIC 300

BLAST of CmUC06G113550 vs. ExPASy TrEMBL
Match: A0A6J1L4G1 (Procollagen-proline 4-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111499059 PE=3 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 3.5e-161
Identity = 282/300 (94.00%), Postives = 291/300 (97.00%), Query Frame = 0

Query: 1   MFKFPNLLFICLILISLVVRESTCSYAGSASSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60
           M KF  LLF+ LILIS VVRES+CSYAGSA+STVDPSKVKQISWKPRAFVYEGFLTDLEC
Sbjct: 1   MSKFRYLLFLFLILISSVVRESSCSYAGSATSTVDPSKVKQISWKPRAFVYEGFLTDLEC 60

Query: 61  DHLVSIARSELKRSEVADNDSGESKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK 120
           DHLVSIARSELKRSEVADNDSG+SKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK
Sbjct: 61  DHLVSIARSELKRSEVADNDSGDSKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPK 120

Query: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPLAEK 180
           ENGEDIQVLRYEHGQKYESHYDYFVDKVNIA GGHRLATVLMYLS+VTKGGETVFPLAEK
Sbjct: 121 ENGEDIQVLRYEHGQKYESHYDYFVDKVNIARGGHRLATVLMYLSNVTKGGETVFPLAEK 180

Query: 181 SRHRRAAETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240
           S  RRA+ETDEDLSECAR+GIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS
Sbjct: 181 SPRRRASETDEDLSECARQGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWS 240

Query: 241 ATKWIHVDSFSKNLGNIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 300
           ATKWIHVDSFSKNLGNIG+CTDLNESCERWAALGECTKNPEYMVGS E+PGYCRRSCRIC
Sbjct: 241 ATKWIHVDSFSKNLGNIGDCTDLNESCERWAALGECTKNPEYMVGSAELPGYCRRSCRIC 300

BLAST of CmUC06G113550 vs. TAIR 10
Match: AT5G18900.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 454.1 bits (1167), Expect = 8.5e-128
Identity = 216/293 (73.72%), Postives = 248/293 (84.64%), Query Frame = 0

Query: 8   LFICLILISLVVRESTCSYAGSASSTVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSIA 67
           L I    I  V+ +S+ S   S+S  V+PSKVKQ+S KPRAFVYEGFLT+LECDH+VS+A
Sbjct: 6   LLISFFAIFSVLLQSSTSLISSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSLA 65

Query: 68  RSELKRSEVADNDSGESKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPKENGEDIQ 127
           ++ LKRS VADNDSGESK S VRTSSG FISK KDPIVSGIEDKI+ WTFLPKENGEDIQ
Sbjct: 66  KASLKRSAVADNDSGESKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDIQ 125

Query: 128 VLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPLAEKSRHRRAA 187
           VLRYEHGQKY++H+DYF DKVNI  GGHR+AT+LMYLS+VTKGGETVFP AE    R  +
Sbjct: 126 VLRYEHGQKYDAHFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVLS 185

Query: 188 ETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIHV 247
           E  EDLS+CA++GIAVKP+KGDALLFF+L P+AIPD  SLHGGCPV+EGEKWSATKWIHV
Sbjct: 186 ENKEDLSDCAKRGIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIHV 245

Query: 248 DSFSKNLGNIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           DSF + +   GNCTD+NESCERWA LGECTKNPEYMVG+ E+PGYCRRSC+ C
Sbjct: 246 DSFDRIVTPSGNCTDMNESCERWAVLGECTKNPEYMVGTTELPGYCRRSCKAC 298

BLAST of CmUC06G113550 vs. TAIR 10
Match: AT3G06300.1 (P4H isoform 2 )

HSP 1 Score: 451.4 bits (1160), Expect = 5.5e-127
Identity = 213/294 (72.45%), Postives = 255/294 (86.73%), Query Frame = 0

Query: 7   LLFICLILISLVVRESTCSYAGSASSTVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSI 66
           LLF+ ++L+  +++ STC    S SS ++PSKVKQ+S KPRAFVYEGFLTDLECDHL+S+
Sbjct: 9   LLFVAILLV--LLQSSTC-LISSPSSIINPSKVKQVSSKPRAFVYEGFLTDLECDHLISL 68

Query: 67  ARSELKRSEVADNDSGESKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPKENGEDI 126
           A+  L+RS VADND+GES++S VRTSSG FISK KDPIVSGIEDK++ WTFLPKENGED+
Sbjct: 69  AKENLQRSAVADNDNGESQVSDVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDL 128

Query: 127 QVLRYEHGQKYESHYDYFVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPLAEKSRHRRA 186
           QVLRYEHGQKY++H+DYF DKVNIA GGHR+ATVL+YLS+VTKGGETVFP A++   R  
Sbjct: 129 QVLRYEHGQKYDAHFDYFHDKVNIARGGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSL 188

Query: 187 AETDEDLSECARKGIAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIH 246
           +E  +DLS+CA+KGIAVKPKKG+ALLFF+L+ +AIPD  SLHGGCPV+EGEKWSATKWIH
Sbjct: 189 SENKDDLSDCAKKGIAVKPKKGNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIH 248

Query: 247 VDSFSKNLGNIGNCTDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           VDSF K L + GNCTD+NESCERWA LGEC KNPEYMVG+PE+PG CRRSC+ C
Sbjct: 249 VDSFDKILTHDGNCTDVNESCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

BLAST of CmUC06G113550 vs. TAIR 10
Match: AT3G28480.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 363.6 bits (932), Expect = 1.5e-100
Identity = 166/272 (61.03%), Postives = 209/272 (76.84%), Query Frame = 0

Query: 29  SASSTVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSIARSELKRSEVADNDSGESKLST 88
           ++S   DP++V Q+SW PR F+YEGFL+D ECDH + +A+ +L++S VADNDSGES  S 
Sbjct: 46  ASSFGFDPTRVTQLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESE 105

Query: 89  VRTSSGMFISKSKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEHGQKYESHYDYFVDKV 148
           VRTSSGMF+SK +D IVS +E K+AAWTFLP+ENGE +Q+L YE+GQKYE H+DYF D+ 
Sbjct: 106 VRTSSGMFLSKRQDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQA 165

Query: 149 NIAWGGHRLATVLMYLSDVTKGGETVFPLAEKSRHRRAAETDEDLSECARKGIAVKPKKG 208
           N+  GGHR+ATVLMYLS+V KGGETVFP+    + +     D+  +ECA++G AVKP+KG
Sbjct: 166 NLELGGHRIATVLMYLSNVEKGGETVFPM---WKGKATQLKDDSWTECAKQGYAVKPRKG 225

Query: 209 DALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIHVDSFSKNLGNIGNCTDLNESCE 268
           DALLFF+L PNA  D+NSLHG CPV+EGEKWSAT+WIHV SF +       C D N SCE
Sbjct: 226 DALLFFNLHPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSGCMDENVSCE 285

Query: 269 RWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           +WA  GEC KNP YMVGS +  GYCR+SC+ C
Sbjct: 286 KWAKAGECQKNPTYMVGSDKDHGYCRKSCKAC 314

BLAST of CmUC06G113550 vs. TAIR 10
Match: AT3G28480.2 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 341.3 bits (874), Expect = 8.0e-94
Identity = 160/280 (57.14%), Postives = 203/280 (72.50%), Query Frame = 0

Query: 29  SASSTVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSIARSELKRSEVADNDSGES---- 88
           ++S   DP++V Q+SW PR F+YEGFL+D ECDH + +A+ +L++S VADNDSGES    
Sbjct: 46  ASSFGFDPTRVTQLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESE 105

Query: 89  -KLSTVRTSSGMFISKSK---DPIVSGIEDKIAAWTFLPKENGEDIQVLRYEHGQKYESH 148
             +S VR SS    +      D IVS +E K+AAWTFLP+ENGE +Q+L YE+GQKYE H
Sbjct: 106 DSVSVVRQSSSFIANMDSLEIDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPH 165

Query: 149 YDYFVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPLAEKSRHRRAAETDEDLSECARKG 208
           +DYF D+ N+  GGHR+ATVLMYLS+V KGGETVFP+    + +     D+  +ECA++G
Sbjct: 166 FDYFHDQANLELGGHRIATVLMYLSNVEKGGETVFPM---WKGKATQLKDDSWTECAKQG 225

Query: 209 IAVKPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIHVDSFSKNLGNIGNC 268
            AVKP+KGDALLFF+L PNA  D+NSLHG CPV+EGEKWSAT+WIHV SF +       C
Sbjct: 226 YAVKPRKGDALLFFNLHPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSGC 285

Query: 269 TDLNESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
            D N SCE+WA  GEC KNP YMVGS +  GYCR+SC+ C
Sbjct: 286 MDENVSCEKWAKAGECQKNPTYMVGSDKDHGYCRKSCKAC 322

BLAST of CmUC06G113550 vs. TAIR 10
Match: AT3G28490.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 340.9 bits (873), Expect = 1.0e-93
Identity = 163/277 (58.84%), Postives = 208/277 (75.09%), Query Frame = 0

Query: 25  SYAGSASSTVDPSKVKQISWKPRAFVYEGFLTDLECDHLVSIARSELKRS-EVADNDSGE 84
           S   S S +VDP+++ Q+SW PRAF+Y+GFL+D ECDHL+ +A+ +L++S  VAD DSGE
Sbjct: 18  SQISSFSFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGE 77

Query: 85  SKLSTVRTSSGMFISKSKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEHGQKYESHYDY 144
           S+ S VRTSSGMF++K +D IV+ +E K+AAWTFLP+ENGE +Q+L YE+GQKY+ H+DY
Sbjct: 78  SEDSEVRTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDY 137

Query: 145 FVDKVNIAWGGHRLATVLMYLSDVTKGGETVFPLAEKSRHRRAAETDEDLSECARKGIAV 204
           F DK  +  GGHR+ATVLMYLS+VTKGGETVFP     + +     D+  S+CA++G AV
Sbjct: 138 FYDKKALELGGHRIATVLMYLSNVTKGGETVFP---NWKGKTPQLKDDSWSKCAKQGYAV 197

Query: 205 KPKKGDALLFFSLEPNAIPDTNSLHGGCPVLEGEKWSATKWIHVDSFSKNLGNIGNCTDL 264
           KP+KGDALLFF+L  N   D NSLHG CPV+EGEKWSAT+WIHV SF K       C D 
Sbjct: 198 KPRKGDALLFFNLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHVRSFGKKK---LVCVDD 257

Query: 265 NESCERWAALGECTKNPEYMVGSPEMPGYCRRSCRIC 301
           +ESC+ WA  GEC KNP YMVGS    G+CR+SC+ C
Sbjct: 258 HESCQEWADAGECEKNPMYMVGSETSLGFCRKSCKAC 288

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038874583.12.7e-16897.33probable prolyl 4-hydroxylase 4 [Benincasa hispida][more]
XP_008458517.16.2e-16595.00PREDICTED: probable prolyl 4-hydroxylase 4 [Cucumis melo] >XP_008458518.1 PREDIC... [more]
XP_004144967.15.3e-16494.00probable prolyl 4-hydroxylase 4 [Cucumis sativus] >XP_011656650.1 probable proly... [more]
KAG6575033.12.2e-16294.33putative prolyl 4-hydroxylase 4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022959148.12.9e-16294.33probable prolyl 4-hydroxylase 4 [Cucurbita moschata] >XP_022959149.1 probable pr... [more]
Match NameE-valueIdentityDescription
Q8LAN31.2e-12673.72Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=... [more]
F4JAU37.8e-12672.45Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1[more]
Q8L9702.1e-9961.03Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=... [more]
F4J0A81.5e-9258.84Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=... [more]
Q9LN201.3e-6454.55Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A1S3C8163.0e-16595.00Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103497901 PE=3 S... [more]
A0A5A7SVW63.0e-16595.00Procollagen-proline 4-dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A0A0KCQ52.6e-16494.00Procollagen-proline 4-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_6G067350 PE=... [more]
A0A6J1H5451.4e-16294.33Procollagen-proline 4-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111460227 ... [more]
A0A6J1L4G13.5e-16194.00Procollagen-proline 4-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111499059 PE... [more]
Match NameE-valueIdentityDescription
AT5G18900.18.5e-12873.722-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G06300.15.5e-12772.45P4H isoform 2 [more]
AT3G28480.11.5e-10061.03Oxoglutarate/iron-dependent oxygenase [more]
AT3G28480.28.0e-9457.14Oxoglutarate/iron-dependent oxygenase [more]
AT3G28490.11.0e-9358.84Oxoglutarate/iron-dependent oxygenase [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003582ShKT domainSMARTSM00254ShkT_1coord: 259..300
e-value: 0.0067
score: 25.6
IPR003582ShKT domainPFAMPF01549ShKcoord: 259..300
e-value: 4.8E-6
score: 27.0
IPR003582ShKT domainPROSITEPS51670SHKTcoord: 260..300
score: 8.880531
IPR006620Prolyl 4-hydroxylase, alpha subunitSMARTSM00702p4hccoord: 46..246
e-value: 3.5E-63
score: 225.9
IPR044862Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domainPFAMPF136402OG-FeII_Oxy_3coord: 126..246
e-value: 6.7E-20
score: 71.8
NoneNo IPR availableGENE3D2.60.120.620q2cbj1_9rhob like domaincoord: 38..247
e-value: 2.6E-75
score: 254.7
NoneNo IPR availablePANTHERPTHR10869:SF175PROLYL 4-HYDROXYLASE SUBUNIT ALPHA-LIKE PROTEINcoord: 8..300
IPR045054Prolyl 4-hydroxylasePANTHERPTHR10869PROLYL 4-HYDROXYLASE ALPHA SUBUNITcoord: 8..300
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 122..247
score: 12.301916

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmUC06G113550.1CmUC06G113550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0018401 peptidyl-proline hydroxylation to 4-hydroxy-L-proline
cellular_component GO:0005789 endoplasmic reticulum membrane
molecular_function GO:0005506 iron ion binding
molecular_function GO:0031418 L-ascorbic acid binding
molecular_function GO:0004656 procollagen-proline 4-dioxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen