CmUC05G101050 (gene) Watermelon (USVL531) v1

Overview


Name	CmUC05G101050
Type	gene
Organism	Citrullus mucosospermus (Watermelon (USVL531) v1)
Description	calmodulin-like
Location	CmU531Chr05: 30163146 .. 30163418 (-)
RNA-Seq Expression	CmUC05G101050
Synteny	CmUC05G101050

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAGGAAAAGCAAAACGTACCATCCCAGGGCGAAGCTATCAAGAGAGCAGTTACTTGTAATCTTCAGAAACCACGACAGCGACAAGGACGGCCGCCTCAGCACCACCGAACTCACCGAAGCCTTCCGATATATCGGCGCCTTTCTCCCGTCCTACAGAGCTCGCCACGCTCTTTTTCATTCCGATTCCGACAGAGATGGATTTATCGGAGAGAAGGATTTCGAGTTGCTCCTCAATTATGCCGAGCAACGCCAATATACCATCATTTGA

mRNA sequence

ATGGCGAGGAAAAGCAAAACGTACCATCCCAGGGCGAAGCTATCAAGAGAGCAGTTACTTGTAATCTTCAGAAACCACGACAGCGACAAGGACGGCCGCCTCAGCACCACCGAACTCACCGAAGCCTTCCGATATATCGGCGCCTTTCTCCCGTCCTACAGAGCTCGCCACGCTCTTTTTCATTCCGATTCCGACAGAGATGGATTTATCGGAGAGAAGGATTTCGAGTTGCTCCTCAATTATGCCGAGCAACGCCAATATACCATCATTTGA

Coding sequence (CDS)

ATGGCGAGGAAAAGCAAAACGTACCATCCCAGGGCGAAGCTATCAAGAGAGCAGTTACTTGTAATCTTCAGAAACCACGACAGCGACAAGGACGGCCGCCTCAGCACCACCGAACTCACCGAAGCCTTCCGATATATCGGCGCCTTTCTCCCGTCCTACAGAGCTCGCCACGCTCTTTTTCATTCCGATTCCGACAGAGATGGATTTATCGGAGAGAAGGATTTCGAGTTGCTCCTCAATTATGCCGAGCAACGCCAATATACCATCATTTGA

Protein sequence

MARKSKTYHPRAKLSREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALFHSDSDRDGFIGEKDFELLLNYAEQRQYTII

Homology

[Close]

BLAST of CmUC05G101050 vs. NCBI nr
Match: KAA0064376.1 (putative calcium-binding protein CML27 [Cucumis melo var. makuwa] >TYK20211.1 putative calcium-binding protein CML27 [Cucumis melo var. makuwa])

HSP 1 Score: 172.2 bits (435), Expect = 2.1e-39
Identity = 81/90 (90.00%), Postives = 88/90 (97.78%), Query Frame = 0


Query: 1  MARKSKTYHPRAKLSREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALF 60
          M+R+SKTYHPRA+LSREQLL+IFRNHDSDKDGRLSTTELTEAFRYIGAFLP YRARHALF
Sbjct: 1  MSRRSKTYHPRARLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPPYRARHALF 60

Query: 61 HSDSDRDGFIGEKDFELLLNYAEQRQYTII 91
          H+DSDRDGFI EKDFELL+NYAEQRQYT+I
Sbjct: 61 HADSDRDGFISEKDFELLVNYAEQRQYTVI 90

[Close]

BLAST of CmUC05G101050 vs. NCBI nr
Match: KGN55185.1 (hypothetical protein Csa_012915 [Cucumis sativus])

HSP 1 Score: 169.1 bits (427), Expect = 1.8e-38
Identity = 81/90 (90.00%), Postives = 87/90 (96.67%), Query Frame = 0


Query: 1  MARKSKTYHPRAKLSREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALF 60
          M+RKSK Y+PRAKLSREQLL+IFRNHDSDKDGRLSTTELTEAFRYIGAFLP+YRARHAL 
Sbjct: 1  MSRKSKNYYPRAKLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPTYRARHALL 60

Query: 61 HSDSDRDGFIGEKDFELLLNYAEQRQYTII 91
          H+DSDRDGFIGEKDFE L+NYAEQRQYTII
Sbjct: 61 HADSDRDGFIGEKDFESLVNYAEQRQYTII 90

[Close]

BLAST of CmUC05G101050 vs. NCBI nr
Match: KAG7024248.1 (Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 156.4 bits (394), Expect = 1.2e-34
Identity = 76/90 (84.44%), Postives = 81/90 (90.00%), Query Frame = 0


Query: 1  MARKSKTYHPRAKLSREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALF 60
          MARKSKTY PR KLSREQL VIFRNHD D DGRLSTTELT AFRYIGAFLPSYRA HAL 
Sbjct: 1  MARKSKTYQPRPKLSREQLSVIFRNHDGDGDGRLSTTELTAAFRYIGAFLPSYRALHALL 60

Query: 61 HSDSDRDGFIGEKDFELLLNYAEQRQYTII 91
          H+DSD DGFIGEKDFELL+NYAE+R+YT+I
Sbjct: 61 HTDSDGDGFIGEKDFELLVNYAEKRKYTVI 90

[Close]

BLAST of CmUC05G101050 vs. NCBI nr
Match: KAG6591370.1 (Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 154.1 bits (388), Expect = 5.9e-34
Identity = 75/88 (85.23%), Postives = 79/88 (89.77%), Query Frame = 0


Query: 1  MARKSKTYHPRAKLSREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALF 60
          MARKSKTY PR KLSREQL VIFRNHD D DGRLSTTELT AFRYIGAFLPSYRA HAL 
Sbjct: 1  MARKSKTYQPRPKLSREQLSVIFRNHDGDGDGRLSTTELTAAFRYIGAFLPSYRALHALL 60

Query: 61 HSDSDRDGFIGEKDFELLLNYAEQRQYT 89
          H+DSD DGFIGEKDFELL+NYAE+R+YT
Sbjct: 61 HTDSDGDGFIGEKDFELLVNYAEKRKYT 88

[Close]

BLAST of CmUC05G101050 vs. NCBI nr
Match: KAG6608481.1 (Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037808.1 putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 145.6 bits (366), Expect = 2.1e-31
Identity = 71/90 (78.89%), Postives = 77/90 (85.56%), Query Frame = 0


Query: 1  MARKSKTYHPRAKLSREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALF 60
          MARK+K   PR KLSREQL VIFRNHD D DGRLS TELTEAFRYIGAFLPSYRA HAL 
Sbjct: 1  MARKNKMSQPRPKLSREQLSVIFRNHDGDGDGRLSATELTEAFRYIGAFLPSYRAHHALR 60

Query: 61 HSDSDRDGFIGEKDFELLLNYAEQRQYTII 91
          ++DSDRDGFIGEKDFE L+NY E+R YTI+
Sbjct: 61 YTDSDRDGFIGEKDFESLVNYTEKRHYTIV 90

[Close]

BLAST of CmUC05G101050 vs. ExPASy Swiss-Prot
Match: Q39419 (Polcalcin Bet v 4 OS=Betula pendula OX=3505 GN=BETV4 PE=1 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 2.0e-05
Identity = 25/67 (37.31%), Postives = 40/67 (59.70%), Query Frame = 0


Query: 9  HPRAKLSREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALFHSDSDRDG 68
          HP+ K  RE+   IF+  D++ DG++S  EL EA + +G+  P    +H +   D+D DG
Sbjct: 5  HPQDKAERER---IFKRFDANGDGKISAAELGEALKTLGSITPD-EVKHMMAEIDTDGDG 64

Query: 69 FIGEKDF 76
          FI  ++F
Sbjct: 65 FISFQEF 67

[Close]

BLAST of CmUC05G101050 vs. ExPASy Swiss-Prot
Match: Q8L3R2 (Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 PE=2 SV=2)

HSP 1 Score: 49.3 bits (116), Expect = 2.7e-05
Identity = 23/61 (37.70%), Postives = 37/61 (60.66%), Query Frame = 0


Query: 15  SREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALFHSDSDRDGFIGEKD 74
           S+E+L  +F + DSD DG++S  EL   F  +G ++    A+ A+   D+D DG +G +D
Sbjct: 61  SKEELRQVFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFED 120

Query: 75  F 76
           F
Sbjct: 121 F 121

[Close]

BLAST of CmUC05G101050 vs. ExPASy TrEMBL
Match: A0A5A7VDB9 (Putative calcium-binding protein CML27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003480 PE=4 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 1.0e-39
Identity = 81/90 (90.00%), Postives = 88/90 (97.78%), Query Frame = 0


Query: 1  MARKSKTYHPRAKLSREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALF 60
          M+R+SKTYHPRA+LSREQLL+IFRNHDSDKDGRLSTTELTEAFRYIGAFLP YRARHALF
Sbjct: 1  MSRRSKTYHPRARLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPPYRARHALF 60

Query: 61 HSDSDRDGFIGEKDFELLLNYAEQRQYTII 91
          H+DSDRDGFI EKDFELL+NYAEQRQYT+I
Sbjct: 61 HADSDRDGFISEKDFELLVNYAEQRQYTVI 90

[Close]

BLAST of CmUC05G101050 vs. ExPASy TrEMBL
Match: A0A0A0KZR6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639730 PE=4 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 8.5e-39
Identity = 81/90 (90.00%), Postives = 87/90 (96.67%), Query Frame = 0


Query: 1  MARKSKTYHPRAKLSREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALF 60
          M+RKSK Y+PRAKLSREQLL+IFRNHDSDKDGRLSTTELTEAFRYIGAFLP+YRARHAL 
Sbjct: 1  MSRKSKNYYPRAKLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPTYRARHALL 60

Query: 61 HSDSDRDGFIGEKDFELLLNYAEQRQYTII 91
          H+DSDRDGFIGEKDFE L+NYAEQRQYTII
Sbjct: 61 HADSDRDGFIGEKDFESLVNYAEQRQYTII 90

[Close]

BLAST of CmUC05G101050 vs. ExPASy TrEMBL
Match: A0A5C7H618 (Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_021566 PE=4 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 5.2e-12
Identity = 36/81 (44.44%), Postives = 54/81 (66.67%), Query Frame = 0


Query: 9   HPRAKLSREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALFHSDSDRDG 68
           HP    + +QL  IFR  D+D DGRLS  ELT+AF  +G+  P+YRA  AL H+D+++DG
Sbjct: 103 HPNLPYNEKQLHGIFRRFDTDHDGRLSQQELTDAFHSLGSRFPAYRAWRALHHADANKDG 162

Query: 69  FIGEKDFELLLNYAEQRQYTI 90
           F+ E +F+ L+ YA +  + +
Sbjct: 163 FVSENEFQQLVKYAVKHGFLL 183

[Close]

BLAST of CmUC05G101050 vs. ExPASy TrEMBL
Match: A0A5N6RQV2 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_018544 PE=4 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 5.2e-12
Identity = 38/75 (50.67%), Postives = 55/75 (73.33%), Query Frame = 0


Query: 15 SREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALFHSDSDRDGFIGEKD 74
          +R+QL+ IFR+HD D DG+LS  E+  AF Y+G+   SYRA  AL H+DSD DG++GE++
Sbjct: 16 TRDQLMNIFRSHDLDGDGKLSRKEVKAAFGYLGSRWDSYRAVLALLHADSDHDGYVGEEE 75

Query: 75 FELLLNYAEQRQYTI 90
           + L++YA +  YTI
Sbjct: 76 LKKLVDYALKCGYTI 90

[Close]

BLAST of CmUC05G101050 vs. ExPASy TrEMBL
Match: A5AWP8 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_023174 PE=4 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 8.9e-12
Identity = 37/84 (44.05%), Postives = 57/84 (67.86%), Query Frame = 0


Query: 6  KTYHPRAKLSREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALFHSDSD 65
          KT   ++ L  +QL  IF+ HD++ DG+L+  EL +AF+++GA +P +RA  AL H+D+D
Sbjct: 7  KTIPSKSPLPEQQLKKIFKQHDTNGDGQLNKAELKQAFQHLGALIPGWRAHRALHHADTD 66

Query: 66 RDGFIGEKDFELLLNYAEQRQYTI 90
           DG I EK+ + L+ YA +  YTI
Sbjct: 67 GDGCISEKEMKDLVEYAAKFGYTI 90

[Close]

BLAST of CmUC05G101050 vs. TAIR 10
Match: AT3G50770.1 (calmodulin-like 41 )

HSP 1 Score: 49.3 bits (116), Expect = 1.9e-06
Identity = 23/61 (37.70%), Postives = 37/61 (60.66%), Query Frame = 0


Query: 15  SREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALFHSDSDRDGFIGEKD 74
           S+E+L  +F + DSD DG++S  EL   F  +G ++    A+ A+   D+D DG +G +D
Sbjct: 61  SKEELRQVFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFED 120

Query: 75  F 76
           F
Sbjct: 121 F 121

[Close]

BLAST of CmUC05G101050 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 42.7 bits (99), Expect = 1.8e-04
Identity = 21/61 (34.43%), Postives = 35/61 (57.38%), Query Frame = 0


Query: 19 LLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALFHSDSDRDGFIGEKDFELL 78
          L  +F   D+++DGR+S  EL ++F+ +G  +    A  A+  SD D DG +   +F LL
Sbjct: 23 LEAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALL 82

Query: 79 L 80
          +
Sbjct: 83 I 83

[Close]

BLAST of CmUC05G101050 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 42.4 bits (98), Expect = 2.3e-04
Identity = 21/69 (30.43%), Postives = 39/69 (56.52%), Query Frame = 0


Query: 18  QLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALFHSDSDRDGFIGEKDFEL 77
           +L  +F   D+++DGR+S  EL ++F  +G  L    A  A+  SD+D DG +  ++F  
Sbjct: 43  ELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQ 102

Query: 78  LLNYAEQRQ 87
           L+   ++ +
Sbjct: 103 LIKVDDEEE 111

[Close]

BLAST of CmUC05G101050 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 42.4 bits (98), Expect = 2.3e-04
Identity = 21/69 (30.43%), Postives = 39/69 (56.52%), Query Frame = 0


Query: 18  QLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALFHSDSDRDGFIGEKDFEL 77
           +L  +F   D+++DGR+S  EL ++F  +G  L    A  A+  SD+D DG +  ++F  
Sbjct: 43  ELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQ 102

Query: 78  LLNYAEQRQ 87
           L+   ++ +
Sbjct: 103 LIKVDDEEE 111

The following BLAST results are available for this feature:

BLAST of CmUC05G101050 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0064376.1	2.1e-39	90.00	putative calcium-binding protein CML27 [Cucumis melo var. makuwa] >TYK20211.1 pu...	[more]
KGN55185.1	1.8e-38	90.00	hypothetical protein Csa_012915 [Cucumis sativus]	[more]
KAG7024248.1	1.2e-34	84.44	Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6591370.1	5.9e-34	85.23	Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG6608481.1	2.1e-31	78.89	Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037808.1...	[more]

BLAST of CmUC05G101050 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39419	2.0e-05	37.31	Polcalcin Bet v 4 OS=Betula pendula OX=3505 GN=BETV4 PE=1 SV=1	[more]
Q8L3R2	2.7e-05	37.70	Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 ...	[more]

BLAST of CmUC05G101050 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VDB9	1.0e-39	90.00	Putative calcium-binding protein CML27 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A0A0KZR6	8.5e-39	90.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639730 PE=4 SV=1	[more]
A0A5C7H618	5.2e-12	44.44	Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_021566 PE=4 SV=1	[more]
A0A5N6RQV2	5.2e-12	50.67	Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_018544 PE=4 SV=1	[more]
A5AWP8	8.9e-12	44.05	Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_023174 PE=4 SV=1	[more]

BLAST of CmUC05G101050 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G50770.1	1.9e-06	37.70	calmodulin-like 41	[more]
AT1G76640.1	1.8e-04	34.43	Calcium-binding EF-hand family protein	[more]
AT1G76650.1	2.3e-04	30.43	calmodulin-like 38	[more]
AT1G76650.3	2.3e-04	30.43	calmodulin-like 38	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.238.10		coord: 3..88 e-value: 2.7E-9 score: 38.7
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 19..44 e-value: 3.7E-5 score: 23.2
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 14..49 score: 12.365459
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 63..75
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 27..39
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 13..81

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmUC05G101050.1	CmUC05G101050.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding