CmUC05G100450 (gene) Watermelon (USVL531) v1

Overview


Name	CmUC05G100450
Type	gene
Organism	Citrullus mucosospermus (Watermelon (USVL531) v1)
Description	cytochrome P450 CYP82D47-like
Location	CmU531Chr05: 29742575 .. 29743314 (-)
RNA-Seq Expression	CmUC05G100450
Synteny	CmUC05G100450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAATTCTTCACATCCTAGCGGCAAGCTTCACCCTTCTCCTATTTCTCTACGCTCTCTTTCGTACTACATCTCGGACAAAGAACAAACTTCCACCGGAAGCCAGCGGCGGTTTGCCGGTGATCGGCCACCTCCATAAACTAATAATTGCAAAAGAGCCAACTCACAAAACCTTGGCCAAAATGGCCGGCGTCTACGGACCAATCTTTACCTTAAGATTAGGCATGAACACAGCCCTAATCGTGAGTAGTTGGAAATAAACGACAGAATCTTCGCATCCCGCCCGAAAGCTTTAGCCTCAGAGCTGCTAGGCTACAACTATGCAGCAATGGAGGTTAGCCAGTATGGCCCATATTGGCGCCATATTCGCAAAATAATCACACTCGAACTCCTCACAAACCACCGCCTCGAGCAGTGCCAACACATTAGAATATCCGAGGTTCAAACTTCGATCAAGAAACTGTATGAGTTATGTGGGGATGGCAGAAATAATAACAAGGGAACAATGGAGATGAAGACGTGGTTTGAGGAGCTAAAAATGAGTATAATATTGAGGATAATGGTAGGAAAGCGATTCAGCTCGGCTTTTGAAGGCAGTGTTGGGGAGCAGTTTCGAGGAGCGTTGAGGGATCTTCTTTATCTGTTTGGGGCATTTGTTCCTTCAGATTCGTTTCCGTTTCTAAGTTGGTTGGATTTAGGAGGGTATAAGAAAGCCATGAAGAAGACGGCGAAGGTGTAG

mRNA sequence

ATGGAAATTCTTCACATCCTAGCGGCAAGCTTCACCCTTCTCCTATTTCTCTACGCTCTCTTTCGTACTACATCTCGGACAAAGAACAAACTTCCACCGGAAGCCAGCGGCGGTTTGCCGTTGGAAATAAACGACAGAATCTTCGCATCCCGCCCGAAAGCTTTAGCCTCAGAGCTGCTAGGCTACAACTATGCAGCAATGGAGGTTAGCCAGTATGGCCCATATTGGCGCCATATTCGCAAAATAATCACACTCGAACTCCTCACAAACCACCGCCTCGAGCAGTGCCAACACATTAGAATATCCGAGGTTCAAACTTCGATCAAGAAACTGTATGAGTTATGTGGGGATGGCAGAAATAATAACAAGGGAACAATGGAGATGAAGACGTGGTTTGAGGAGCTAAAAATGAGTATAATATTGAGGATAATGGTAGGAAAGCGATTCAGCTCGGCTTTTGAAGGCAGTGTTGGGGAGCAGTTTCGAGGAGCGTTGAGGGATCTTCTTTATCTGTTTGGGGCATTTGTTCCTTCAGATTCGTTTCCGTTTCTAAGTTGGTTGGATTTAGGAGGGTATAAGAAAGCCATGAAGAAGACGGCGAAGGTGTAG

Coding sequence (CDS)

ATGGAAATTCTTCACATCCTAGCGGCAAGCTTCACCCTTCTCCTATTTCTCTACGCTCTCTTTCGTACTACATCTCGGACAAAGAACAAACTTCCACCGGAAGCCAGCGGCGGTTTGCCGTTGGAAATAAACGACAGAATCTTCGCATCCCGCCCGAAAGCTTTAGCCTCAGAGCTGCTAGGCTACAACTATGCAGCAATGGAGGTTAGCCAGTATGGCCCATATTGGCGCCATATTCGCAAAATAATCACACTCGAACTCCTCACAAACCACCGCCTCGAGCAGTGCCAACACATTAGAATATCCGAGGTTCAAACTTCGATCAAGAAACTGTATGAGTTATGTGGGGATGGCAGAAATAATAACAAGGGAACAATGGAGATGAAGACGTGGTTTGAGGAGCTAAAAATGAGTATAATATTGAGGATAATGGTAGGAAAGCGATTCAGCTCGGCTTTTGAAGGCAGTGTTGGGGAGCAGTTTCGAGGAGCGTTGAGGGATCTTCTTTATCTGTTTGGGGCATTTGTTCCTTCAGATTCGTTTCCGTTTCTAAGTTGGTTGGATTTAGGAGGGTATAAGAAAGCCATGAAGAAGACGGCGAAGGTGTAG

Protein sequence

MEILHILAASFTLLLFLYALFRTTSRTKNKLPPEASGGLPLEINDRIFASRPKALASELLGYNYAAMEVSQYGPYWRHIRKIITLELLTNHRLEQCQHIRISEVQTSIKKLYELCGDGRNNNKGTMEMKTWFEELKMSIILRIMVGKRFSSAFEGSVGEQFRGALRDLLYLFGAFVPSDSFPFLSWLDLGGYKKAMKKTAKV

Homology

[Close]

BLAST of CmUC05G100450 vs. NCBI nr
Match: XP_023516810.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 260.8 bits (665), Expect = 1.0e-65
Identity = 144/257 (56.03%), Postives = 165/257 (64.20%), Query Frame = 0


Query: 1   MEILHILAASFTLLLFLYALFR-----TTSRTKNKLPPEASGGLPL-------------- 60
           ME+ HILAA+F LLLFLYALF       T RT  +LPPEA G LP+              
Sbjct: 1   MELTHILAANFALLLFLYALFHIAWTSITHRTNKRLPPEAGGALPVIGHLHKLSTTEPTH 60

Query: 61  ------------------------------------EINDRIFASRPKALASELLGYNYA 120
                                                 NDRIFASR K LAS+LL YNYA
Sbjct: 61  KTLAKMADAYGPIFTIRLGMSRALIVSNWEIVRECFTTNDRIFASRSKTLASKLLAYNYA 120

Query: 121 AMEVSQYGPYWRHIRKIITLELLTNHRLEQCQHIRISEVQTSIKKLYELCGDGRNNNKGT 180
           A+ VS YGPYWRH+RKI TL+LLTNHRLEQ QHIRISEVQ+SIKKL+ELC D  +N K  
Sbjct: 121 AVGVSSYGPYWRHVRKITTLKLLTNHRLEQLQHIRISEVQSSIKKLHELCADKGSNGKVL 180

Query: 181 MEMKTWFEELKMSIILRIMVGKRFSSAFEGSVGEQFRGALRDLLYLFGAFVPSDSFPFLS 203
           +EMKTWF +L ++ I +++VGKRFS+ FEGS   Q R ALRD L LFG FVPSDSFPFLS
Sbjct: 181 VEMKTWFGDLTLNTIFKMVVGKRFSTDFEGSDAAQCREALRDFLELFGVFVPSDSFPFLS 240

[Close]

BLAST of CmUC05G100450 vs. NCBI nr
Match: XP_022935741.1 (cytochrome P450 CYP82D47-like [Cucurbita moschata])

HSP 1 Score: 258.5 bits (659), Expect = 4.9e-65
Identity = 145/261 (55.56%), Postives = 162/261 (62.07%), Query Frame = 0


Query: 1   MEILHILAASFTLLLFLYALFRTTS------RTKNKLPPEASGGLPL------------- 60
           ME+ HIL A+F LLLFLYALF  TS      RT N LPPE  G LPL             
Sbjct: 1   MELTHILPATFALLLFLYALFAITSRRSAAHRTNNTLPPEPPGRLPLIGHLHKLSATEPT 60

Query: 61  -------------------------------------EINDRIFASRPKALASELLGYNY 120
                                                  ND IFASRPK LA ELLGYN 
Sbjct: 61  HITLAKMADAYGPIFTLRLGVNTALVVSSWEIARECFTTNDTIFASRPKGLAPELLGYNS 120

Query: 121 AAMEVSQYGPYWRHIRKIITLELLTNHRLEQCQHIRISEVQTSIKKLYELCGDGRNNNKG 180
           A M +S YGPYWRH+RK+ TLELLTNHRLEQ QHIRISEVQ+SIKKLYELC D  +   G
Sbjct: 121 AMMAISPYGPYWRHVRKLATLELLTNHRLEQLQHIRISEVQSSIKKLYELCADSTSKGSG 180

Query: 181 ---TMEMKTWFEELKMSIILRIMVGKRFSSAFEGSVGEQFRGALRDLLYLFGAFVPSDSF 203
               +EM+ WF +L ++ I R+MVGK+FS+  EGS GEQ+RGALRD+  LFGAFVPSDSF
Sbjct: 181 GKVRVEMRKWFGDLSLNTIFRMMVGKQFSTDLEGSGGEQYRGALRDVFELFGAFVPSDSF 240

[Close]

BLAST of CmUC05G100450 vs. NCBI nr
Match: XP_023535446.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 255.4 bits (651), Expect = 4.2e-64
Identity = 144/261 (55.17%), Postives = 162/261 (62.07%), Query Frame = 0


Query: 1   MEILHILAASFTLLLFLYALFRTTS------RTKNKLPPEASGGLPL------------- 60
           ME+ HIL A+F LLLFLYALF  TS      RT N LPPE +G LPL             
Sbjct: 1   MELTHILPATFALLLFLYALFAITSRRSAAHRTNNTLPPEPAGRLPLIGHLHKLSATEPT 60

Query: 61  -------------------------------------EINDRIFASRPKALASELLGYNY 120
                                                  ND IFASRPK LA ELLGYN 
Sbjct: 61  HITLAKMADAYGPIFTLRLGVNTALVVSSWEIARECFTTNDTIFASRPKGLAPELLGYNS 120

Query: 121 AAMEVSQYGPYWRHIRKIITLELLTNHRLEQCQHIRISEVQTSIKKLYELCGDGRNNNKG 180
           A M +S YGPYWRH+RKI TLELLTNHRLEQ QHIRISEVQ+SIKKLYELC D  +   G
Sbjct: 121 AMMAISPYGPYWRHVRKIATLELLTNHRLEQLQHIRISEVQSSIKKLYELCADSTSKGSG 180

Query: 181 ---TMEMKTWFEELKMSIILRIMVGKRFSSAFEGSVGEQFRGALRDLLYLFGAFVPSDSF 203
               +EM+TWF ++ ++ I R+MVGKRFS+  EGS GE++R ALRD+  LFGAFVPSDSF
Sbjct: 181 GKVRVEMETWFGDVSLNTIFRMMVGKRFSTDSEGSGGEKYRQALRDVFELFGAFVPSDSF 240

[Close]

BLAST of CmUC05G100450 vs. NCBI nr
Match: XP_022976390.1 (cytochrome P450 CYP82D47-like [Cucurbita maxima])

HSP 1 Score: 254.2 bits (648), Expect = 9.3e-64
Identity = 141/261 (54.02%), Postives = 162/261 (62.07%), Query Frame = 0


Query: 1   MEILHILAASFTLLLFLYALFRTTS------RTKNKLPPEASGGLPL------------- 60
           ME+ HIL A+F LLLFLYALFR +S      RT   LPPEA+G LP+             
Sbjct: 1   MELTHILPATFALLLFLYALFRISSRRSAAHRTNKTLPPEAAGRLPVIGHLHKLSATEPT 60

Query: 61  -------------------------------------EINDRIFASRPKALASELLGYNY 120
                                                  ND IFASRPK LASELLGYNY
Sbjct: 61  HITLAKMADAYGPIFTLRLGMNTALIVSSWEIARECFTTNDTIFASRPKGLASELLGYNY 120

Query: 121 AAMEVSQYGPYWRHIRKIITLELLTNHRLEQCQHIRISEVQTSIKKLYELCGDGRNNNKG 180
             + +S YGPYWRH+RKI T ELL N RLEQ QHIR+SEV++SIK LYELCGD RN   G
Sbjct: 121 TVLSLSPYGPYWRHVRKIATHELLANQRLEQLQHIRMSEVRSSIKTLYELCGDSRNKGSG 180

Query: 181 ---TMEMKTWFEELKMSIILRIMVGKRFSSAFEGSVGEQFRGALRDLLYLFGAFVPSDSF 203
               +EM+TWF ++ ++ ILR++VGKR S  FEGS  EQ+R A RD+  LFGAFVPSDSF
Sbjct: 181 GKVRVEMRTWFGDVSLNTILRMVVGKRLSFVFEGSGAEQYREAFRDVFELFGAFVPSDSF 240

[Close]

BLAST of CmUC05G100450 vs. NCBI nr
Match: XP_022975796.1 (cytochrome P450 82A3-like [Cucurbita maxima])

HSP 1 Score: 253.1 bits (645), Expect = 2.1e-63
Identity = 142/261 (54.41%), Postives = 161/261 (61.69%), Query Frame = 0


Query: 1   MEILHILAASFTLLLFLYALFRTTS------RTKNKLPPEASGGLPL------------- 60
           MEI HIL A F LLL LYALFR  S      RT   LPPEA+G LPL             
Sbjct: 1   MEITHILPAIFALLLLLYALFRIASPRSAANRTNKTLPPEAAGRLPLIGHLHKLSATEPT 60

Query: 61  -------------------------------------EINDRIFASRPKALASELLGYNY 120
                                                  ND IFASRPK LA ELLGYN 
Sbjct: 61  HITLAKMADVYGPIFTLRLGMNTALVVSSWEIARECFTTNDTIFASRPKGLAPELLGYNS 120

Query: 121 AAMEVSQYGPYWRHIRKIITLELLTNHRLEQCQHIRISEVQTSIKKLYELCGDGRNNNKG 180
           A M +S YGPYWRH+RK+ TLELLTNHRLEQ QHIRISEVQ+SIKKLYELC D  +   G
Sbjct: 121 AMMAISPYGPYWRHVRKLATLELLTNHRLEQLQHIRISEVQSSIKKLYELCADSTSKGSG 180

Query: 181 ---TMEMKTWFEELKMSIILRIMVGKRFSSAFEGSVGEQFRGALRDLLYLFGAFVPSDSF 203
               +EM+ WF ++ ++ I R++VGKRFS+ FEGS GEQ+R ALRD+  LFGAFVPSDSF
Sbjct: 181 GKVRVEMRKWFGDVSLNTIFRMVVGKRFSAVFEGSGGEQYRRALRDVFELFGAFVPSDSF 240

[Close]

BLAST of CmUC05G100450 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 5.2e-33
Identity = 70/163 (42.94%), Postives = 106/163 (65.03%), Query Frame = 0


Query: 44  NDRIFASRPKALASELLGYNYAAMEVSQYGPYWRHIRKIITLELLTNHRLEQCQHIRISE 103
           ND   +SRPK +A E++ YN A + ++ YGPYWR +RKI+T E L+N R+EQ  HIR+SE
Sbjct: 100 NDLAVSSRPKLVAVEVMSYNQAFVGLAPYGPYWRELRKIVTFEFLSNRRIEQRNHIRVSE 159

Query: 104 VQTSIKKLYELCGDGRNNNK--GTMEMKTWFEELKMSIILRIMVGKRFSSAF--EG-SVG 163
           V+TSIK+L+++  +G  N      +++K W   L  ++++R++VGKR+      EG    
Sbjct: 160 VRTSIKELFDIWSNGNKNESRYTLVDIKQWLAYLTFNMVVRMVVGKRYFGVMHVEGKDKA 219

Query: 164 EQFRGALRDLLYLFGAFVPSDSFPFLSWLDLGGYKKAMKKTAK 202
           ++F   +R+ + L G F  +D  P L WLDLGG++KAMK  AK
Sbjct: 220 QRFMKNIREFMNLMGTFTVADGVPCLRWLDLGGHEKAMKANAK 262

[Close]

BLAST of CmUC05G100450 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 6.8e-33
Identity = 72/160 (45.00%), Postives = 108/160 (67.50%), Query Frame = 0


Query: 44  NDRIFASRPKALASELLGYNYAAMEVSQYGPYWRHIRKIITLELLTNHRLEQCQHIRISE 103
           ND   ++RPK L +EL+ YN A + V+ YGPYWR +RKII  E+L++ R+EQ Q +R+SE
Sbjct: 102 NDVAVSARPKLLVAELMCYNNAMLLVAPYGPYWRELRKIIVTEILSSSRVEQLQDVRVSE 161

Query: 104 VQTSIKKLYELCGDGRN-NNKGTMEMKTWFEELKMSIILRIMVGKRFSSA-FEGSVGEQF 163
           VQ SI +LY++    +N ++  ++E+K WF +   +++LR++VGKRF SA       E+ 
Sbjct: 162 VQNSIVELYDVWRSQKNESDYASVELKQWFAQPIFNMVLRMVVGKRFLSATATDEKAEKC 221

Query: 164 RGALRDLLYLFGAFVPSDSFPFLSWLDLGGYKKAMKKTAK 202
             A+ + + L G F   D+ P+L WLD GGY+KAMK+TAK
Sbjct: 222 VKAVDEFMRLAGVFTVGDAIPYLRWLDFGGYEKAMKETAK 261

[Close]

BLAST of CmUC05G100450 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 8.8e-33
Identity = 69/163 (42.33%), Postives = 108/163 (66.26%), Query Frame = 0


Query: 44  NDRIFASRPKALASELLGYNYAAMEVSQYGPYWRHIRKIITLELLTNHRLEQCQHIRISE 103
           NDR F +RP  +A++++GYNY  + V+ YGPYWR +RKII LE L+N RL+  +H+  SE
Sbjct: 98  NDRFFLNRPSGVAAKIMGYNYVMLGVAPYGPYWRDMRKIIMLEFLSNRRLQSLKHVWHSE 157

Query: 104 VQTSIKKLYELCGDGRNNNKGTMEMKTWFEELKMSIILRIMVGKRFSSAFEGSVGEQFRG 163
           +  S K+LY+L  + +N +   ++MK W  +L +++ ++++VGKRF  +   S  E+   
Sbjct: 158 ISISSKELYKL-WETQNIDFCLVDMKQWLADLTLNMSVKMVVGKRFFGSASASACEETES 217

Query: 164 A-----LRDLLYLFGAFVPSDSFPFLSWLDLGGYKKAMKKTAK 202
           +     LR++  L G+FV SD  P+L WLDLGG++K MK+T K
Sbjct: 218 SNCPKTLRNMFRLMGSFVLSDYLPYLRWLDLGGHEKEMKRTVK 259

[Close]

BLAST of CmUC05G100450 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 139.8 bits (351), Expect = 3.4e-32
Identity = 76/181 (41.99%), Postives = 109/181 (60.22%), Query Frame = 0


Query: 44  NDRIFASRPKALASELLGYNYAAMEVSQYGPYWRHIRKIITLELLTNHRLEQCQHIRISE 103
           ND + +SRPK +A EL+ YN A +  + YG YWR +RKI+TLE+L+N R+E   HIR+SE
Sbjct: 97  NDIVVSSRPKPVAVELMSYNQAFIGWAPYGAYWRQLRKIVTLEILSNRRIELLSHIRVSE 156

Query: 104 VQTSIKKL-----------YELCGDGRNNNKG---------TMEMKTWFEELKMSIILRI 163
           VQTSIK+L           Y L  D ++++           ++E+K WF +L ++++LR+
Sbjct: 157 VQTSIKELVNVWSNQISSQYGLLDDTKSSSTNDEPSTTDYVSVELKKWFAQLTLNMVLRM 216

Query: 164 MVGKRFSSAFEGSVGEQ---FRGALRDLLYLFGAFVPSDSFPFLSWLDLGGYKKAMKKTA 202
           +VGKR     +    E+   F   +RD + L G F   D  PFL WLDLGG++K MKK A
Sbjct: 217 VVGKRCFGDVDVENKEEAKRFLENIRDFMRLIGTFTVGDGVPFLKWLDLGGHEKEMKKCA 276

[Close]

BLAST of CmUC05G100450 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 3.8e-28
Identity = 61/164 (37.20%), Postives = 99/164 (60.37%), Query Frame = 0


Query: 43  INDRIFASRPKALASELLGYNYAAMEVSQYGPYWRHIRKIITLELLTNHRLEQCQHIRIS 102
           +ND+  ASRP   A++ +GYN+A    + Y  +WR +RKI T+ELL+N RL+  +H+R+S
Sbjct: 94  VNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWREMRKIATIELLSNRRLQMLKHVRVS 153

Query: 103 EVQTSIKKLYELCGDGRNNNKGTMEMKTWFEELKMSIILRIMVGKRFSSAFEGSVGE--- 162
           E+   +K LY L           +++K+W E++ +++I+R++ GKR+     GSV     
Sbjct: 154 EITMGVKDLYSLWFKNGGTKPVMVDLKSWLEDMTLNMIVRMVAGKRYFGG-GGSVSSEDT 213

Query: 163 ----QFRGALRDLLYLFGAFVPSDSFPFLSWLDLGGYKKAMKKT 200
               Q + A+    +L G F  SD+FP LS+ DL G++K MK+T
Sbjct: 214 EEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQGHEKEMKQT 256

[Close]

BLAST of CmUC05G100450 vs. ExPASy TrEMBL
Match: A0A6J1F5K0 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442564 PE=3 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 2.4e-65
Identity = 145/261 (55.56%), Postives = 162/261 (62.07%), Query Frame = 0


Query: 1   MEILHILAASFTLLLFLYALFRTTS------RTKNKLPPEASGGLPL------------- 60
           ME+ HIL A+F LLLFLYALF  TS      RT N LPPE  G LPL             
Sbjct: 1   MELTHILPATFALLLFLYALFAITSRRSAAHRTNNTLPPEPPGRLPLIGHLHKLSATEPT 60

Query: 61  -------------------------------------EINDRIFASRPKALASELLGYNY 120
                                                  ND IFASRPK LA ELLGYN 
Sbjct: 61  HITLAKMADAYGPIFTLRLGVNTALVVSSWEIARECFTTNDTIFASRPKGLAPELLGYNS 120

Query: 121 AAMEVSQYGPYWRHIRKIITLELLTNHRLEQCQHIRISEVQTSIKKLYELCGDGRNNNKG 180
           A M +S YGPYWRH+RK+ TLELLTNHRLEQ QHIRISEVQ+SIKKLYELC D  +   G
Sbjct: 121 AMMAISPYGPYWRHVRKLATLELLTNHRLEQLQHIRISEVQSSIKKLYELCADSTSKGSG 180

Query: 181 ---TMEMKTWFEELKMSIILRIMVGKRFSSAFEGSVGEQFRGALRDLLYLFGAFVPSDSF 203
               +EM+ WF +L ++ I R+MVGK+FS+  EGS GEQ+RGALRD+  LFGAFVPSDSF
Sbjct: 181 GKVRVEMRKWFGDLSLNTIFRMMVGKQFSTDLEGSGGEQYRGALRDVFELFGAFVPSDSF 240

[Close]

BLAST of CmUC05G100450 vs. ExPASy TrEMBL
Match: A0A6J1IJB7 (cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111476808 PE=3 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 4.5e-64
Identity = 141/261 (54.02%), Postives = 162/261 (62.07%), Query Frame = 0


Query: 1   MEILHILAASFTLLLFLYALFRTTS------RTKNKLPPEASGGLPL------------- 60
           ME+ HIL A+F LLLFLYALFR +S      RT   LPPEA+G LP+             
Sbjct: 1   MELTHILPATFALLLFLYALFRISSRRSAAHRTNKTLPPEAAGRLPVIGHLHKLSATEPT 60

Query: 61  -------------------------------------EINDRIFASRPKALASELLGYNY 120
                                                  ND IFASRPK LASELLGYNY
Sbjct: 61  HITLAKMADAYGPIFTLRLGMNTALIVSSWEIARECFTTNDTIFASRPKGLASELLGYNY 120

Query: 121 AAMEVSQYGPYWRHIRKIITLELLTNHRLEQCQHIRISEVQTSIKKLYELCGDGRNNNKG 180
             + +S YGPYWRH+RKI T ELL N RLEQ QHIR+SEV++SIK LYELCGD RN   G
Sbjct: 121 TVLSLSPYGPYWRHVRKIATHELLANQRLEQLQHIRMSEVRSSIKTLYELCGDSRNKGSG 180

Query: 181 ---TMEMKTWFEELKMSIILRIMVGKRFSSAFEGSVGEQFRGALRDLLYLFGAFVPSDSF 203
               +EM+TWF ++ ++ ILR++VGKR S  FEGS  EQ+R A RD+  LFGAFVPSDSF
Sbjct: 181 GKVRVEMRTWFGDVSLNTILRMVVGKRLSFVFEGSGAEQYREAFRDVFELFGAFVPSDSF 240

[Close]

BLAST of CmUC05G100450 vs. ExPASy TrEMBL
Match: A0A6J1IF60 (cytochrome P450 82A3-like OS=Cucurbita maxima OX=3661 GN=LOC111476389 PE=3 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 1.0e-63
Identity = 142/261 (54.41%), Postives = 161/261 (61.69%), Query Frame = 0


Query: 1   MEILHILAASFTLLLFLYALFRTTS------RTKNKLPPEASGGLPL------------- 60
           MEI HIL A F LLL LYALFR  S      RT   LPPEA+G LPL             
Sbjct: 1   MEITHILPAIFALLLLLYALFRIASPRSAANRTNKTLPPEAAGRLPLIGHLHKLSATEPT 60

Query: 61  -------------------------------------EINDRIFASRPKALASELLGYNY 120
                                                  ND IFASRPK LA ELLGYN 
Sbjct: 61  HITLAKMADVYGPIFTLRLGMNTALVVSSWEIARECFTTNDTIFASRPKGLAPELLGYNS 120

Query: 121 AAMEVSQYGPYWRHIRKIITLELLTNHRLEQCQHIRISEVQTSIKKLYELCGDGRNNNKG 180
           A M +S YGPYWRH+RK+ TLELLTNHRLEQ QHIRISEVQ+SIKKLYELC D  +   G
Sbjct: 121 AMMAISPYGPYWRHVRKLATLELLTNHRLEQLQHIRISEVQSSIKKLYELCADSTSKGSG 180

Query: 181 ---TMEMKTWFEELKMSIILRIMVGKRFSSAFEGSVGEQFRGALRDLLYLFGAFVPSDSF 203
               +EM+ WF ++ ++ I R++VGKRFS+ FEGS GEQ+R ALRD+  LFGAFVPSDSF
Sbjct: 181 GKVRVEMRKWFGDVSLNTIFRMVVGKRFSAVFEGSGGEQYRRALRDVFELFGAFVPSDSF 240

[Close]

BLAST of CmUC05G100450 vs. ExPASy TrEMBL
Match: A0A6J1F6F9 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442567 PE=3 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 2.2e-63
Identity = 140/261 (53.64%), Postives = 162/261 (62.07%), Query Frame = 0


Query: 1   MEILHILAASFTLLLFLYALFRTTS------RTKNKLPPEASGGLPL------------- 60
           ME+ HILAA+F LLLFLYALFR TS      RT N LPPEA+G LP+             
Sbjct: 1   MELTHILAATFPLLLFLYALFRITSRRSAAHRTNNTLPPEAAGRLPVIGHLHKLSATEPT 60

Query: 61  -------------------------------------EINDRIFASRPKALASELLGYNY 120
                                                  NDRIFASR K +ASELLGYNY
Sbjct: 61  HITLARMADAYGPIFTLKLGVNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNY 120

Query: 121 AAMEVSQYGPYWRHIRKIITLELLTNHRLEQCQHIRISEVQTSIKKLYELCGDGRNNNKG 180
           AA+ +S YGPYWRH+RK+ T+ELLTNHRLEQ QHIRI EVQ SI+KLYELC D RN   G
Sbjct: 121 AALGISPYGPYWRHVRKLATVELLTNHRLEQLQHIRICEVQNSIEKLYELCADSRNKGSG 180

Query: 181 ---TMEMKTWFEELKMSIILRIMVGKRFSSAFEGSVGEQFRGALRDLLYLFGAFVPSDSF 203
               +EM+ WF ++ ++ I R++VGKRFS+ FEG+  EQ+R ALR    LF AFVPSDSF
Sbjct: 181 GKVRVEMRRWFGDVSLNTIFRMVVGKRFSAVFEGNGAEQYREALRAFFKLFAAFVPSDSF 240

[Close]

BLAST of CmUC05G100450 vs. ExPASy TrEMBL
Match: A0A6J1F723 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442948 PE=3 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 2.9e-63
Identity = 140/262 (53.44%), Postives = 160/262 (61.07%), Query Frame = 0


Query: 1   MEILHILAASFTLLLFLYALFRTTS-------RTKNKLPPEASGGLPL------------ 60
           ME+ HIL A+F LLLFLYALFR  S       R+  + PPEA+G LPL            
Sbjct: 1   MELTHILPATFALLLFLYALFRIASRRSSAAHRSNKRHPPEAAGALPLIGHLHKLSATEP 60

Query: 61  --------------------------------------EINDRIFASRPKALASELLGYN 120
                                                   ND IFASR K +ASELLGYN
Sbjct: 61  THITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDTIFASRSKVVASELLGYN 120

Query: 121 YAAMEVSQYGPYWRHIRKIITLELLTNHRLEQCQHIRISEVQTSIKKLYELCGDGRNNNK 180
           YAA+ +S YGPYWRH+RK+ TLELLTNHRLEQ QHIRI EVQ SIKKLYELC D RN   
Sbjct: 121 YAALGISPYGPYWRHVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGS 180

Query: 181 G---TMEMKTWFEELKMSIILRIMVGKRFSSAFEGSVGEQFRGALRDLLYLFGAFVPSDS 203
           G    +EM+ WF ++ ++ I R++VGKRFS+ FEGS  EQ+R ALR    LF AFVPSD 
Sbjct: 181 GGKVRVEMRRWFGDVSLNTIFRMVVGKRFSAVFEGSGAEQYREALRAFFELFAAFVPSDL 240

[Close]

BLAST of CmUC05G100450 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 126.3 bits (316), Expect = 2.7e-29
Identity = 61/164 (37.20%), Postives = 99/164 (60.37%), Query Frame = 0


Query: 43  INDRIFASRPKALASELLGYNYAAMEVSQYGPYWRHIRKIITLELLTNHRLEQCQHIRIS 102
           +ND+  ASRP   A++ +GYN+A    + Y  +WR +RKI T+ELL+N RL+  +H+R+S
Sbjct: 94  VNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWREMRKIATIELLSNRRLQMLKHVRVS 153

Query: 103 EVQTSIKKLYELCGDGRNNNKGTMEMKTWFEELKMSIILRIMVGKRFSSAFEGSVGE--- 162
           E+   +K LY L           +++K+W E++ +++I+R++ GKR+     GSV     
Sbjct: 154 EITMGVKDLYSLWFKNGGTKPVMVDLKSWLEDMTLNMIVRMVAGKRYFGG-GGSVSSEDT 213

Query: 163 ----QFRGALRDLLYLFGAFVPSDSFPFLSWLDLGGYKKAMKKT 200
               Q + A+    +L G F  SD+FP LS+ DL G++K MK+T
Sbjct: 214 EEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQGHEKEMKQT 256

[Close]

BLAST of CmUC05G100450 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 120.2 bits (300), Expect = 2.0e-27
Identity = 56/164 (34.15%), Postives = 97/164 (59.15%), Query Frame = 0


Query: 43  INDRIFASRPKALASELLGYNYAAMEVSQYGPYWRHIRKIITLELLTNHRLEQCQHIRIS 102
           +ND+  ASRP   A++ +GY+ A    + Y  +WR +RKI TLELL+N RL+  +H+R+S
Sbjct: 94  VNDKALASRPITAAAKHMGYDCAVFGFAPYSAFWREMRKIATLELLSNRRLQMLKHVRVS 153

Query: 103 EVQTSIKKLYELCGDGRNNNKGTMEMKTWFEELKMSIILRIMVGKRF-----SSAFEGSV 162
           E+   ++ LY L      +    +++K+W E++ +++++R++ GKR+      S  +   
Sbjct: 154 EISMVMQDLYSLWVKKGGSEPVMVDLKSWLEDMSLNMMVRMVAGKRYFGGGSLSPEDAEE 213

Query: 163 GEQFRGALRDLLYLFGAFVPSDSFPFLSWLDLGGYKKAMKKTAK 202
             Q R  + +  +L G F  SD+FP L W D  G++K MK+T +
Sbjct: 214 ARQCRKGVANFFHLVGIFTVSDAFPKLGWFDFQGHEKEMKQTGR 257

[Close]

BLAST of CmUC05G100450 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 100.5 bits (249), Expect = 1.6e-21
Identity = 57/145 (39.31%), Postives = 79/145 (54.48%), Query Frame = 0


Query: 44  NDRIFASRPKALASELLGYNYAAMEVSQYGPYWRHIRKIITLELLTNHRLEQCQHIRISE 103
           ND   A+RP       +GYN A++ ++ YG YWR +RKI+T+ L +NH +E   HIR SE
Sbjct: 101 NDLATATRPNIAFGRYVGYNNASLTLAPYGDYWRELRKIVTVHLFSNHSIEMLGHIRSSE 160

Query: 104 VQTSIKKLYELCGDGRNNNKGTMEMKTWFEELKMSIILRIMVGKRFSSAFEGSVGEQFRG 163
           V T IK LY+      N     +++   FE L  +IILR MVGKR       S   +++ 
Sbjct: 161 VNTLIKHLYK-----GNGGTSIVKIDMLFEFLTFNIILRKMVGKRIGFGEVNSDEWRYKE 220

Query: 164 ALRDLLYLFGAFVPSDSFPFLSWLD 189
           AL+   YL    +  D  P+L WLD
Sbjct: 221 ALKHCEYLAVIPMIGDVIPWLGWLD 240

[Close]

BLAST of CmUC05G100450 vs. TAIR 10
Match: AT3G25180.2 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 100.5 bits (249), Expect = 1.6e-21
Identity = 57/145 (39.31%), Postives = 79/145 (54.48%), Query Frame = 0


Query: 44  NDRIFASRPKALASELLGYNYAAMEVSQYGPYWRHIRKIITLELLTNHRLEQCQHIRISE 103
           ND   A+RP       +GYN A++ ++ YG YWR +RKI+T+ L +NH +E   HIR SE
Sbjct: 101 NDLATATRPNIAFGRYVGYNNASLTLAPYGDYWRELRKIVTVHLFSNHSIEMLGHIRSSE 160

Query: 104 VQTSIKKLYELCGDGRNNNKGTMEMKTWFEELKMSIILRIMVGKRFSSAFEGSVGEQFRG 163
           V T IK LY+      N     +++   FE L  +IILR MVGKR       S   +++ 
Sbjct: 161 VNTLIKHLYK-----GNGGTSIVKIDMLFEFLTFNIILRKMVGKRIGFGEVNSDEWRYKE 220

Query: 164 ALRDLLYLFGAFVPSDSFPFLSWLD 189
           AL+   YL    +  D  P+L WLD
Sbjct: 221 ALKHCEYLAVIPMIGDVIPWLGWLD 240

[Close]

BLAST of CmUC05G100450 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 96.7 bits (239), Expect = 2.3e-20
Identity = 65/213 (30.52%), Postives = 104/213 (48.83%), Query Frame = 0


Query: 4   LHILAASFTLLLFLYALFRTTSRTKNKLPP---------EASGGLPLEINDRIFASRPKA 63
           LH+L     L      L+RT  +  +   P         E   G   E+    F    KA
Sbjct: 45  LHLLGGKEQL------LYRTLGKMADHYGPAMSLRLGSSETFVGSSFEVAKDCFTVNDKA 104

Query: 64  LASELLGYNYAAMEVSQYGPYWRHIRKIITLELLTNHRLEQCQHIRISEVQTSIKKLYEL 123
           LAS +     AA +   Y  +W  +RKI  +ELL+N RL+   ++R+SE+   +K LY L
Sbjct: 105 LASLMT----AAAKHMGY-VFWLEMRKIAMIELLSNRRLQMLNNVRVSEISMGVKDLYSL 164

Query: 124 CGDGRNNNKGTMEMKTWFEELKMSIILRIMVGKRF------SSAFEGSVGEQFRGALRDL 183
                 +    +++K+W E++  ++I+R++ GKR+       S+       Q+R  +   
Sbjct: 165 WVKKGGSEPVMVDLKSWLEDMIANMIMRMVAGKRYFGGGGAESSEHTEEARQWRKGIAKF 224

Query: 184 LYLFGAFVPSDSFPFLSWLDLGGYKKAMKKTAK 202
            +L G F  SD+FP L WLDL G++K MK+T +
Sbjct: 225 FHLVGIFTVSDAFPKLGWLDLQGHEKEMKQTRR 246

The following BLAST results are available for this feature:

BLAST of CmUC05G100450 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023516810.1	1.0e-65	56.03	cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo]	[more]
XP_022935741.1	4.9e-65	55.56	cytochrome P450 CYP82D47-like [Cucurbita moschata]	[more]
XP_023535446.1	4.2e-64	55.17	cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo]	[more]
XP_022976390.1	9.3e-64	54.02	cytochrome P450 CYP82D47-like [Cucurbita maxima]	[more]
XP_022975796.1	2.1e-63	54.41	cytochrome P450 82A3-like [Cucurbita maxima]	[more]

BLAST of CmUC05G100450 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49858	5.2e-33	42.94	Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1	[more]
O49859	6.8e-33	45.00	Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1	[more]
A0A0N7F297	8.8e-33	42.33	Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP...	[more]
Q43068	3.4e-32	41.99	Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2	[more]
Q9SZ46	3.8e-28	37.20	Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=...	[more]

BLAST of CmUC05G100450 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F5K0	2.4e-65	55.56	cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442564 PE=3...	[more]
A0A6J1IJB7	4.5e-64	54.02	cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111476808 PE=3 S...	[more]
A0A6J1IF60	1.0e-63	54.41	cytochrome P450 82A3-like OS=Cucurbita maxima OX=3661 GN=LOC111476389 PE=3 SV=1	[more]
A0A6J1F6F9	2.2e-63	53.64	cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442567 PE=3...	[more]
A0A6J1F723	2.9e-63	53.44	cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442948 PE=3...	[more]

BLAST of CmUC05G100450 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G31940.1	2.7e-29	37.20	cytochrome P450, family 82, subfamily C, polypeptide 4	[more]
AT4G31970.1	2.0e-27	34.15	cytochrome P450, family 82, subfamily C, polypeptide 2	[more]
AT3G25180.1	1.6e-21	39.31	cytochrome P450, family 82, subfamily G, polypeptide 1	[more]
AT3G25180.2	1.6e-21	39.31	cytochrome P450, family 82, subfamily G, polypeptide 1	[more]
AT4G31950.1	2.3e-20	30.52	cytochrome P450, family 82, subfamily C, polypeptide 3	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 41..202 e-value: 9.3E-21 score: 75.9
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 44..198
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 70..198 e-value: 5.2E-7 score: 28.8
None	No IPR available	PANTHER	PTHR24281	STEROID 21-HYDROXYLASE-RELATED	coord: 42..201
None	No IPR available	PANTHER	PTHR24281:SF48	CYTOCHROME P450	coord: 42..201

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmUC05G100450.1	CmUC05G100450.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen