CmUC05G099120 (gene) Watermelon (USVL531) v1

Overview
NameCmUC05G099120
Typegene
OrganismCitrullus mucosospermus (Watermelon (USVL531) v1)
Description28S rRNA (Cytosine-C(5))-methyltransferase
LocationCmU531Chr05: 28630536 .. 28635439 (+)
RNA-Seq ExpressionCmUC05G099120
SyntenyCmUC05G099120
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAACTTGGCTGAAGGTTATCAAATTCCAAAGTTCAACCTCCTTCATCTTTTGGTAGACATAACTTTTCCCAGAATACCCAACAAATCTTTCTTCTGGAAGGGTTTTATTAATACAGGCTTTTTAATTATGAATTATTCCTAAAACATTAGAACATGTAAGAGTAAAAAAGTAATCGTCCTCTAAATCCTATCCAATCAAGCAAAATTGAACTATCTTAGTTTTTCTTCTAATTTTAAAATGGACTACATTTCTAGGTAAAATAATAGTTCAATTGAACCATTCAGGTAAAAATTATACAAGTTTAAAATTTATTTGGGCTATAGCCCTAATTGGAATTTACACACTGGGCTAAGGCCCGGGATTTTGTCGTCGTTTCACTGCACAGTTGGGATCAGCGACCTTCTCGAGTATCGACTCTCGACCGGAGTTCGGAGTGCTCCTTCCGTGGAGTTATGGGGCGCGTTAACAACAGAACCAACGCATCGGCGTCGGGAACTGCCGGTAAATCAGAAAATCGGCGGATGAGCAACGCCGACCGTTCGGCATACTTCGCAAGGAGAGAGGCGGCGAAGATATTGCGGCGTGTTCTTGAGGGAGATGCTCAGCGTCGAGCCGTCGCCTCCATCAAGTCACTCGTTTATGCTCCGTCTGTTCGGAATAAGAAGGGCACATTCGCTCTTGTCTGCAAGACACTCAAATGTAAGTTCTTTCCCGAAATGATTTTCAAACTGGTCCTCGTGTAGCTGTCAATAGAAGTATTGGTTTTAGTTGAACATCTTGCTCTGCGTTTGGATGTGAGATTTTTAATATTGTTGTGCAACGGAGCCGTGCAGCTTCGAAGTAATTTATATCACAACTATAAATTATATGGAAAATTGAAATCCTACTTATTTGATATGAATCTATGAGAAGCTAGATATTAGCTTTTCCCTCGTTCCTTTGTGCTGATGTTTGCATTGGTGTAGCATAACCATTAAGGTTTATTTTGTAGCTCCCTGAATTCTCTTGCTTTTGTTGTGTTATTAGACCTCCCTGTAATCAAGGATGTACTTGAGGCTGCTGATTTGTTAAGCAATAAATGGAAGGTACTCTTGCATCTTCTTTATAGCTGCTCTCGGTTCTGATCTGATGAAAGTTGTTCTTTTACCACTATCTTTTGTATTTCATTTGTTTCTCAAGTTTTCTCACTTACTCCAAACATGCCATAACACTAGCCTAATGTTGTTGCAGAGGCAGAAGGAATTAATTTATGTCATCATGTATGATATCCTTCTAGGCCAGGTATTTCATTCGCGCATTTATCTTAGTTAAGACGATAACAGTGTATCATTATAAAAGGTCGGCTCGGAAAATATTTTATGACAGTTGAACAAGCGGGAGAAGGACTTATTGTTGTTTGAATTCCATGCCAACTATCAGGACATTATATAATTTATTTACTCGAGATCAATTTGTATGTTATTTGTATGCCAACTCCAATTTAATTTATTTAATCAATTTTAATTTATTTAATTTGTATGTTACTCAAGATCAATAATTTGTATGCCAAATCCAATAATATTTATATGTTATTTGCCAGCATGACCGTAACTTAATTGGCATAAGACATACATTCTTGACCAAGAAGTTAAACGTTCAAATCACCCACCCTGATTTTATGTAGTTAAAAATTTTATGGTTGTGGTTTTGAGTTCAAACGAATTTGTATCGTCAAGAGTTACTGTCTACTGGGTCCCGTAGGAGTCTGGAGGCCAGACCATGTAATTCAACACAAGTGAACAATCTTTTTTTCTTTGGTGAGAGGTTTAGACTTTAGAGAAGCTTGCAATAGAAAATACTCAGACAAGTTATTGTTATAATGAAGTGTTTAATGAATATGCAGAAGCTTCTGCTACTGAGACTACTGCAGATCGCTAATTTTACATTTGGTGATAATTGGTTATTTTATCGGAACTGTGCGTAGAAAACTCATTTAGCTGGCGATGCAGAGAAGTTTCTCATGCGGCAGCAAAGTGCTTTACAATCTGTAGTTACACAGATTCTGTCAAAGAAAAAAGCAAACAATATTGAAGATTTAATAGCTCATGGCGATGATCCTGGTGAAAATTCTGTATAAATTTGTTGATGTGCTGATGTCTACTAACTTAGTTATTAAGCCTATTCCGGTCTGAATTGATAATCATCAACTCAGGTGTTTTAAGGCCTCGTTTTATCCGTGTGAATACTTTGAAGATGGATGTCCACACAGCAATAAAAGAATTAGGCAAAAGATATGCAGTGAGCGCCAAAATCCTTCCTAATCGCTATTGATGTTTATCCCAATAGAGTAGAATTCCTCAATGGTGCTGGTTATTATGTCAAACAGGTTCAGAAGGATGACATGGTTCCCGAGCTCTTGGTACTTCCTCCAGGCAGTGATTTACATGATCATCCTCTGGTTGCAGATGGAAGCATCTTCATGCAAGTAAGTCTCTAGTGCATTATCATGAAGCATATGTTTAAGGCGAAGGGTAGCTGTTTCTTCTCCTTATTGTATAACAACAATTGGGTATTAATTTTTGTAGGGGAAGGCTAGTTCTATGGTTGCAGTAGCCCTTGATCCCAAGCCAGGATGGGAAGTAAGATCATATCACCTGTATTTACTGTCAAGCTCACTTTGGAATTTGGATGGGTTTTTCTGGTTTCTCAACTTCTATTTACTCGCTCTTTTCTACCTTTATTTGAAATTTTAACTAATGCCGAACAACTTATCGTTTTACAAGATGTTCAAACTTATTTTGGGGCATCAACCCCATGTTTTCTTATTGCCTCTTTTCTCTTTACCTCTTTGCCACATGGTATAATTACTTGGGCGTTAATATTTCCCATTTTGACATTGTGTATTATTGAAGGTCCTTGATGCATGTTCAGCTCCGGGCAACAAAACTGTCCACCTTGCCTCCCTCATGAATGGAAAAGGAAGGGTCATTGCATGTGAATTGAATGAAAACAGGGTCAGACGCTTACAAGATACTATCAAACTATCTGGCGCTTCTAGTATCCTTGTCTCGTCTGCATACGGGTGTTGAGATAGCAACTCTCTTGCATTAAATTATTTTCTCTATTTGGTTGAAGACTGAAGAGTAATGTGATGTTATTTTCATGTAACCTGTTACCTTGATTAACCTTAACCTTGATGCAAGATATAGAAGTTTTGCATGGGGATTTCCTTAATCTTAATCCAGAAGATCCTTCATTATCCAAAGTAAGTTTTGCTTAGCTTCACAGTGATAATCTTTTCTTCAATCCCTATGATGAATAACTAAAGCTGCTACATTGTGGCCAGAGACTCAAGAGTTACCCTTTTTTTGCTTCTTTTCTAATGATGACCACTTAGATGTAGTTGGATCGGCCAATCATTACAGCTTTCTTTACAAGGCAATCCATTCCCGTTTTCCCCCTAGGTTTTGACTTTTGACAGAACAATGGAAGTAATACGGCTATCATTAATTAGAGATCATAATACTGTGCGATTTCTAATTTGCTTGAGTAGGTGGTAGAACAAAATCTAATCTTTGTCCAGCGTACTGATATTCATCAGGAAAATGTTATGTTCTTGATATCATCCTCTTTTGACATTTTAAGGTCCGTGCCATTCTTTTGGATCCTTCTTGCTCTGGATCTGGGACTGCGGCTGCTAGATTAGATCATTTGCTTCCGTCCCATGTTGAAGGTAACTCCTAGCCTCGTATTTTGTTCTTTTCCTTTGACAATATGTAGGATTAGAAAATTGAACTTCTGACTTGGGCTATGGCAATTGTTCATTTCAGCTCTAACCTCATGTTAACTGGCAACATCATAGTCTATTATCTGCTATATCTTCTTCTGGTTATTGATTGTTGCCTCCATCATATCGTCATGAATTGTACAGGGAAAACCTCTGAGGATGACCTTGAACGACTGAACAAACTCGCTGCCTTTCAGAAAAAGGCTCTAGCACATGCATTTTCCTGTGAGTTGGCCCCCCCACCTTTTTTTTTAAAAAAAATAATATTTCTAATATTTTTTAGAGCTAAAGAGAAAGTAGAATTAATATATTGAAGAATATTAGAATATCTCAGATGTTCGAAAAGGTCTAAAACTCACACCCCTCACAAATTGCTATCAGTTGCATTCTTTCTCGCGTACCTTTCTGTATTCATCATCTTCTTACCCAAGGGAATTTAAAGAGTTTAAATTATTCCAGACGTCCAAAAAGAAAAGTTGACAAGCAATTAACGCAATATCCAAACGCAAATAGTTACAGTACACTAACCTCTGGGCAATTTATAGAGCAAACCTTTTCTTTTCGCTGCTCGTGACCAAAGTCATCTCATTGCAGTCCCAGCCGTAGAGAGAATTGTTTACAGCACTTGTTCCATTCATCAAGTTGAAAACGAAGATGTCGTTCAGTCATTATTACCTCTTGCTGAATCGTATGGTTTTCAACTGGAAACTCCGTTCCCACAGTGGCAGCGGCGAGGTCTCCCAGTTTTTGCGGGTGGTAAGTATCAACATATTGTTTTTTGTTTTTTCAACTCGCCCTCTTTGATTTAGCTATTTAAATAACTTTATTGCATGATAGAACGTTCTGGATCCGTTTCTCATTATGCAGCTAACCATTTACTTCGAACCGATCCTGTTGAAGACAAAGAAGGGTTCTTCATAGCTCTATTTAAGAAGAAGCAGAACACATCGCACCCATCTTCCCGAATTTCAGATAGAGATAAAAGCTCAAGAAATCGTGGTGGAAATATCAGAAACAGTTGGCCAGTAGCCTGCCCTCCAACCAAGATATCCAAACTGTGGTTGTATACACTTTCGTCAAGGACACGATGGAGAAAGAACCACACTTCATCCGAAACTAGAACAAGAACTTAA

mRNA sequence

ATGAAAACTTGGCTGAAGGCCCGGGATTTTGTCGTCGTTTCACTGCACAGTTGGGATCAGCGACCTTCTCGAGTATCGACTCTCGACCGGAGTTCGGAGTGCTCCTTCCGTGGAGTTATGGGGCGCGTTAACAACAGAACCAACGCATCGGCGTCGGGAACTGCCGGTAAATCAGAAAATCGGCGGATGAGCAACGCCGACCGTTCGGCATACTTCGCAAGGAGAGAGGCGGCGAAGATATTGCGGCGTGTTCTTGAGGGAGATGCTCAGCGTCGAGCCGTCGCCTCCATCAAGTCACTCGTTTATGCTCCGTCTGTTCGGAATAAGAAGGGCACATTCGCTCTTGTCTGCAAGACACTCAAATACCTCCCTGTAATCAAGGATGTACTTGAGGCTGCTGATTTGTTAAGCAATAAATGGAAGAGGCAGAAGGAATTAATTTATGTCATCATGTATGATATCCTTCTAGGCCAGAAAACTCATTTAGCTGGCGATGCAGAGAAGTTTCTCATGCGGCAGCAAAGTGCTTTACAATCTGTAGTTACACAGATTCTGTCAAAGAAAAAAGCAAACAATATTGAAGATTTAATAGCTCATGGCGATGATCCTGGTGTTTTAAGGCCTCGTTTTATCCGTGTGAATACTTTGAAGATGGATGTCCACACAGCAATAAAAGAATTAGGCAAAAGATATGCAGTTCAGAAGGATGACATGGTTCCCGAGCTCTTGGTACTTCCTCCAGGCAGTGATTTACATGATCATCCTCTGGTTGCAGATGGAAGCATCTTCATGCAAGGGAAGGCTAGTTCTATGGTTGCAGTAGCCCTTGATCCCAAGCCAGGATGGGAAGTCCTTGATGCATGTTCAGCTCCGGGCAACAAAACTGTCCACCTTGCCTCCCTCATGAATGGAAAAGGAAGGGTCATTGCATGTGAATTGAATGAAAACAGGGTCAGACGCTTACAAGATACTATCAAACTATCTGGCGCTTCTAATATAGAAGTTTTGCATGGGGATTTCCTTAATCTTAATCCAGAAGATCCTTCATTATCCAAAGTCCGTGCCATTCTTTTGGATCCTTCTTGCTCTGGATCTGGGACTGCGGCTGCTAGATTAGATCATTTGCTTCCGTCCCATGTTGAAGGGAAAACCTCTGAGGATGACCTTGAACGACTGAACAAACTCGCTGCCTTTCAGAAAAAGGCTCTAGCACATGCATTTTCCTTCCCAGCCGTAGAGAGAATTGTTTACAGCACTTGTTCCATTCATCAAGTTGAAAACGAAGATGTCGTTCAGTCATTATTACCTCTTGCTGAATCGTATGGTTTTCAACTGGAAACTCCGTTCCCACAGTGGCAGCGGCGAGGTCTCCCAGTTTTTGCGGGTGCTAACCATTTACTTCGAACCGATCCTGTTGAAGACAAAGAAGGGTTCTTCATAGCTCTATTTAAGAAGAAGCAGAACACATCGCACCCATCTTCCCGAATTTCAGATAGAGATAAAAGCTCAAGAAATCGTGGTGGAAATATCAGAAACAGTTGGCCAGTAGCCTGCCCTCCAACCAAGATATCCAAACTGTGGTTGTATACACTTTCGTCAAGGACACGATGGAGAAAGAACCACACTTCATCCGAAACTAGAACAAGAACTTAA

Coding sequence (CDS)

ATGAAAACTTGGCTGAAGGCCCGGGATTTTGTCGTCGTTTCACTGCACAGTTGGGATCAGCGACCTTCTCGAGTATCGACTCTCGACCGGAGTTCGGAGTGCTCCTTCCGTGGAGTTATGGGGCGCGTTAACAACAGAACCAACGCATCGGCGTCGGGAACTGCCGGTAAATCAGAAAATCGGCGGATGAGCAACGCCGACCGTTCGGCATACTTCGCAAGGAGAGAGGCGGCGAAGATATTGCGGCGTGTTCTTGAGGGAGATGCTCAGCGTCGAGCCGTCGCCTCCATCAAGTCACTCGTTTATGCTCCGTCTGTTCGGAATAAGAAGGGCACATTCGCTCTTGTCTGCAAGACACTCAAATACCTCCCTGTAATCAAGGATGTACTTGAGGCTGCTGATTTGTTAAGCAATAAATGGAAGAGGCAGAAGGAATTAATTTATGTCATCATGTATGATATCCTTCTAGGCCAGAAAACTCATTTAGCTGGCGATGCAGAGAAGTTTCTCATGCGGCAGCAAAGTGCTTTACAATCTGTAGTTACACAGATTCTGTCAAAGAAAAAAGCAAACAATATTGAAGATTTAATAGCTCATGGCGATGATCCTGGTGTTTTAAGGCCTCGTTTTATCCGTGTGAATACTTTGAAGATGGATGTCCACACAGCAATAAAAGAATTAGGCAAAAGATATGCAGTTCAGAAGGATGACATGGTTCCCGAGCTCTTGGTACTTCCTCCAGGCAGTGATTTACATGATCATCCTCTGGTTGCAGATGGAAGCATCTTCATGCAAGGGAAGGCTAGTTCTATGGTTGCAGTAGCCCTTGATCCCAAGCCAGGATGGGAAGTCCTTGATGCATGTTCAGCTCCGGGCAACAAAACTGTCCACCTTGCCTCCCTCATGAATGGAAAAGGAAGGGTCATTGCATGTGAATTGAATGAAAACAGGGTCAGACGCTTACAAGATACTATCAAACTATCTGGCGCTTCTAATATAGAAGTTTTGCATGGGGATTTCCTTAATCTTAATCCAGAAGATCCTTCATTATCCAAAGTCCGTGCCATTCTTTTGGATCCTTCTTGCTCTGGATCTGGGACTGCGGCTGCTAGATTAGATCATTTGCTTCCGTCCCATGTTGAAGGGAAAACCTCTGAGGATGACCTTGAACGACTGAACAAACTCGCTGCCTTTCAGAAAAAGGCTCTAGCACATGCATTTTCCTTCCCAGCCGTAGAGAGAATTGTTTACAGCACTTGTTCCATTCATCAAGTTGAAAACGAAGATGTCGTTCAGTCATTATTACCTCTTGCTGAATCGTATGGTTTTCAACTGGAAACTCCGTTCCCACAGTGGCAGCGGCGAGGTCTCCCAGTTTTTGCGGGTGCTAACCATTTACTTCGAACCGATCCTGTTGAAGACAAAGAAGGGTTCTTCATAGCTCTATTTAAGAAGAAGCAGAACACATCGCACCCATCTTCCCGAATTTCAGATAGAGATAAAAGCTCAAGAAATCGTGGTGGAAATATCAGAAACAGTTGGCCAGTAGCCTGCCCTCCAACCAAGATATCCAAACTGTGGTTGTATACACTTTCGTCAAGGACACGATGGAGAAAGAACCACACTTCATCCGAAACTAGAACAAGAACTTAA

Protein sequence

MKTWLKARDFVVVSLHSWDQRPSRVSTLDRSSECSFRGVMGRVNNRTNASASGTAGKSENRRMSNADRSAYFARREAAKILRRVLEGDAQRRAVASIKSLVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQKTHLAGDAEKFLMRQQSALQSVVTQILSKKKANNIEDLIAHGDDPGVLRPRFIRVNTLKMDVHTAIKELGKRYAVQKDDMVPELLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDPKPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNENRVRRLQDTIKLSGASNIEVLHGDFLNLNPEDPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGKTSEDDLERLNKLAAFQKKALAHAFSFPAVERIVYSTCSIHQVENEDVVQSLLPLAESYGFQLETPFPQWQRRGLPVFAGANHLLRTDPVEDKEGFFIALFKKKQNTSHPSSRISDRDKSSRNRGGNIRNSWPVACPPTKISKLWLYTLSSRTRWRKNHTSSETRTRT
Homology
BLAST of CmUC05G099120 vs. NCBI nr
Match: XP_038898181.1 (28S rRNA (cytosine-C(5))-methyltransferase-like isoform X4 [Benincasa hispida])

HSP 1 Score: 911.8 bits (2355), Expect = 2.9e-261
Identity = 466/505 (92.28%), Postives = 483/505 (95.64%), Query Frame = 0

Query: 40  MGRVNNRTNASASGTAGKSENRRMSNADRSAYFARREAAKILRRVLEGDAQRRAVASIKS 99
           MGRV NRT  SASGTAGKSENRRMSNADRSAYFARREAAKILRRVLEGDAQRRAVASIKS
Sbjct: 1   MGRVTNRTKPSASGTAGKSENRRMSNADRSAYFARREAAKILRRVLEGDAQRRAVASIKS 60

Query: 100 LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 159
           LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK
Sbjct: 61  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 120

Query: 160 THLAGDAEKFLMRQQSALQSVVTQILSKKKANNIEDLIAHGDDPGVLRPRFIRVNTLKMD 219
           THLAGDAEKFLMRQQSALQSVV Q+LSKKKA+NIEDLIA GDDPGV RPRFIRVNTLKM+
Sbjct: 121 THLAGDAEKFLMRQQSALQSVVRQLLSKKKASNIEDLIAPGDDPGVSRPRFIRVNTLKME 180

Query: 220 VHTAIKELGKRYAVQKDDMVPELLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDPK 279
           VHTAIKELGK+YAVQKDDMVP+LLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDPK
Sbjct: 181 VHTAIKELGKKYAVQKDDMVPDLLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDPK 240

Query: 280 PGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNENRVRRLQDTIKLSGASNIEVLHGD 339
           PGWEVLDACSAPGNKTVHLASLM GKGR+IACELNENRV+RLQ TIKLSGASNIEVLHGD
Sbjct: 241 PGWEVLDACSAPGNKTVHLASLMKGKGRIIACELNENRVKRLQHTIKLSGASNIEVLHGD 300

Query: 340 FLNLNPEDPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGKTSEDDLERLNKLAAFQ 399
           FLNLNP+DPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGKTSEDDLERLNKLAAFQ
Sbjct: 301 FLNLNPKDPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGKTSEDDLERLNKLAAFQ 360

Query: 400 KKALAHAFSFPAVERIVYSTCSIHQVENEDVVQSLLPLAESYGFQLETPFPQWQRRGLPV 459
           +KALAHAFSFPAVERIVYSTCSIHQVENEDVVQSLLPLAES+GFQL+TPFPQWQRRGLPV
Sbjct: 361 RKALAHAFSFPAVERIVYSTCSIHQVENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLPV 420

Query: 460 FAGANHLLRTDPVEDKEGFFIALFKKKQNTSHPSSRISDRDKSSRNRGGNIRNSWPVACP 519
           FAGANHLLRTDPVEDKEGFFIALF KKQ+T+ P  R S+RDKS R RG N+RNS  VACP
Sbjct: 421 FAGANHLLRTDPVEDKEGFFIALFTKKQSTTQPFPRRSNRDKSFRYRGRNVRNSRAVACP 480

Query: 520 PTKISKLWL--YTLSSRTRWRKNHT 543
           PTKISKLWL  Y  S+RTRWRKNH+
Sbjct: 481 PTKISKLWLYQYAFSTRTRWRKNHS 505

BLAST of CmUC05G099120 vs. NCBI nr
Match: XP_038898177.1 (28S rRNA (cytosine-C(5))-methyltransferase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 901.7 bits (2329), Expect = 3.0e-258
Identity = 463/505 (91.68%), Postives = 481/505 (95.25%), Query Frame = 0

Query: 40  MGRVNNRTNASASGTAGKSENRRMSNADRSAYFARREAAKILRRVLEGDAQRRAVASIKS 99
           MGRV NRT  SASGTAGKSENRRMSNADRSAYFARREAAKILRRVLEGDAQRRAVASIKS
Sbjct: 1   MGRVTNRTKPSASGTAGKSENRRMSNADRSAYFARREAAKILRRVLEGDAQRRAVASIKS 60

Query: 100 LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 159
           LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK
Sbjct: 61  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 120

Query: 160 THLAGDAEKFLMRQQSALQSVVTQILSKKKANNIEDLIAHGDDPGVLRPRFIRVNTLKMD 219
           THLAGDAEKFLMRQQSALQSVV Q+LSKKKA+NIEDLIA GDDPGV RPRFIRVNTLKM+
Sbjct: 121 THLAGDAEKFLMRQQSALQSVVRQLLSKKKASNIEDLIAPGDDPGVSRPRFIRVNTLKME 180

Query: 220 VHTAIKELGKRYAVQKDDMVPELLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDPK 279
           VHTAIKELGK+YAVQKDDMVP+LLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDPK
Sbjct: 181 VHTAIKELGKKYAVQKDDMVPDLLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDPK 240

Query: 280 PGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNENRVRRLQDTIKLSGASNIEVLHGD 339
           PGWEVLDACSAPGNKTVHLASLM GKGR+IACELNENRV+RLQ TIKLSGAS  +VLHGD
Sbjct: 241 PGWEVLDACSAPGNKTVHLASLMKGKGRIIACELNENRVKRLQHTIKLSGAS--KVLHGD 300

Query: 340 FLNLNPEDPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGKTSEDDLERLNKLAAFQ 399
           FLNLNP+DPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGKTSEDDLERLNKLAAFQ
Sbjct: 301 FLNLNPKDPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGKTSEDDLERLNKLAAFQ 360

Query: 400 KKALAHAFSFPAVERIVYSTCSIHQVENEDVVQSLLPLAESYGFQLETPFPQWQRRGLPV 459
           +KALAHAFSFPAVERIVYSTCSIHQVENEDVVQSLLPLAES+GFQL+TPFPQWQRRGLPV
Sbjct: 361 RKALAHAFSFPAVERIVYSTCSIHQVENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLPV 420

Query: 460 FAGANHLLRTDPVEDKEGFFIALFKKKQNTSHPSSRISDRDKSSRNRGGNIRNSWPVACP 519
           FAGANHLLRTDPVEDKEGFFIALF KKQ+T+ P  R S+RDKS R RG N+RNS  VACP
Sbjct: 421 FAGANHLLRTDPVEDKEGFFIALFTKKQSTTQPFPRRSNRDKSFRYRGRNVRNSRAVACP 480

Query: 520 PTKISKLWL--YTLSSRTRWRKNHT 543
           PTKISKLWL  Y  S+RTRWRKNH+
Sbjct: 481 PTKISKLWLYQYAFSTRTRWRKNHS 503

BLAST of CmUC05G099120 vs. NCBI nr
Match: XP_004141333.2 (probable 28S rRNA (cytosine-C(5))-methyltransferase [Cucumis sativus])

HSP 1 Score: 897.9 bits (2319), Expect = 4.4e-257
Identity = 458/523 (87.57%), Postives = 488/523 (93.31%), Query Frame = 0

Query: 24  RVSTLD--RSSECSFRGVMGRVNNRTNASASGTAGKSENRRMSNADRSAYFARREAAKIL 83
           +VST+D        +   MGRVNNRT ASASG AGKSE RRMSNADRSAYFARREAAK+L
Sbjct: 16  KVSTIDCREFGRGGYWEAMGRVNNRTKASASGAAGKSEMRRMSNADRSAYFARREAAKVL 75

Query: 84  RRVLEGDAQRRAVASIKSLVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWK 143
           RRVLEGDAQRRA+ASIK+LVYAPSVRNKKGTFALVCKTLKYLPVIKDV+EAADLLSNKWK
Sbjct: 76  RRVLEGDAQRRAIASIKTLVYAPSVRNKKGTFALVCKTLKYLPVIKDVVEAADLLSNKWK 135

Query: 144 RQKELIYVIMYDILLGQKTHLAGDAEKFLMRQQSALQSVVTQILSKKKANNIEDLIAHGD 203
           RQKELIYVIMYDILLGQKTHLAGDAEKFLMRQQSALQS VTQ+LSKKKANNIEDLIA GD
Sbjct: 136 RQKELIYVIMYDILLGQKTHLAGDAEKFLMRQQSALQSAVTQLLSKKKANNIEDLIARGD 195

Query: 204 D-PGVLRPRFIRVNTLKMDVHTAIKELGKRYAVQKDDMVPELLVLPPGSDLHDHPLVADG 263
           D PGV RPRF+RVNTLKMDVHTAI+ELGKRYAVQKDDMV +LL+LPPGSDLHDHPLVADG
Sbjct: 196 DGPGVSRPRFVRVNTLKMDVHTAIEELGKRYAVQKDDMVSDLLILPPGSDLHDHPLVADG 255

Query: 264 SIFMQGKASSMVAVALDPKPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNENRVRR 323
           SIF+QGKASSMVAVALDPKPGWEVLDACSAPGNKTVHLASLM+GKGRVIACELNENRV+R
Sbjct: 256 SIFLQGKASSMVAVALDPKPGWEVLDACSAPGNKTVHLASLMHGKGRVIACELNENRVKR 315

Query: 324 LQDTIKLSGASNIEVLHGDFLNLNPEDPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHV 383
           L+ TIKLSGASNI+VLHGDFLNLNP+DPSLSKVRAILLDPSCSGSGTAA RLDHLLPSH 
Sbjct: 316 LRHTIKLSGASNIDVLHGDFLNLNPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHA 375

Query: 384 EGKTSEDDLERLNKLAAFQKKALAHAFSFPAVERIVYSTCSIHQVENEDVVQSLLPLAES 443
           EG  S DDLERLNKLAAFQ+KALAHAFSFPAVE+IVYSTCSIHQ+ENEDVVQS+LPLAES
Sbjct: 376 EGTISGDDLERLNKLAAFQRKALAHAFSFPAVEKIVYSTCSIHQIENEDVVQSVLPLAES 435

Query: 444 YGFQLETPFPQWQRRGLPVFAGANHLLRTDPVEDKEGFFIALFKKKQNTSHPSSRISDRD 503
            GFQL+TPFP+WQRRGLPVFAGANHLLRTDPVEDKEGFFIALFKKKQNT++P  R S+RD
Sbjct: 436 RGFQLDTPFPRWQRRGLPVFAGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSSNRD 495

Query: 504 KSSRNRGGNIRNSWPVACPPTKISKLWL--YTLSSRTRWRKNH 542
           KSSR RGG++ NSW VACPPTKISKLWL  Y LS+RTRWRKNH
Sbjct: 496 KSSRYRGGDVCNSWAVACPPTKISKLWLYQYALSTRTRWRKNH 538

BLAST of CmUC05G099120 vs. NCBI nr
Match: KAE8649915.1 (hypothetical protein Csa_012918 [Cucumis sativus])

HSP 1 Score: 897.1 bits (2317), Expect = 7.5e-257
Identity = 454/505 (89.90%), Postives = 481/505 (95.25%), Query Frame = 0

Query: 40  MGRVNNRTNASASGTAGKSENRRMSNADRSAYFARREAAKILRRVLEGDAQRRAVASIKS 99
           MGRVNNRT ASASG AGKSE RRMSNADRSAYFARREAAK+LRRVLEGDAQRRA+ASIK+
Sbjct: 1   MGRVNNRTKASASGAAGKSEMRRMSNADRSAYFARREAAKVLRRVLEGDAQRRAIASIKT 60

Query: 100 LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 159
           LVYAPSVRNKKGTFALVCKTLKYLPVIKDV+EAADLLSNKWKRQKELIYVIMYDILLGQK
Sbjct: 61  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVVEAADLLSNKWKRQKELIYVIMYDILLGQK 120

Query: 160 THLAGDAEKFLMRQQSALQSVVTQILSKKKANNIEDLIAHGDD-PGVLRPRFIRVNTLKM 219
           THLAGDAEKFLMRQQSALQS VTQ+LSKKKANNIEDLIA GDD PGV RPRF+RVNTLKM
Sbjct: 121 THLAGDAEKFLMRQQSALQSAVTQLLSKKKANNIEDLIARGDDGPGVSRPRFVRVNTLKM 180

Query: 220 DVHTAIKELGKRYAVQKDDMVPELLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDP 279
           DVHTAI+ELGKRYAVQKDDMV +LL+LPPGSDLHDHPLVADGSIF+QGKASSMVAVALDP
Sbjct: 181 DVHTAIEELGKRYAVQKDDMVSDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP 240

Query: 280 KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNENRVRRLQDTIKLSGASNIEVLHG 339
           KPGWEVLDACSAPGNKTVHLASLM+GKGRVIACELNENRV+RL+ TIKLSGASNI+VLHG
Sbjct: 241 KPGWEVLDACSAPGNKTVHLASLMHGKGRVIACELNENRVKRLRHTIKLSGASNIDVLHG 300

Query: 340 DFLNLNPEDPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGKTSEDDLERLNKLAAF 399
           DFLNLNP+DPSLSKVRAILLDPSCSGSGTAA RLDHLLPSH EG  S DDLERLNKLAAF
Sbjct: 301 DFLNLNPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHAEGTISGDDLERLNKLAAF 360

Query: 400 QKKALAHAFSFPAVERIVYSTCSIHQVENEDVVQSLLPLAESYGFQLETPFPQWQRRGLP 459
           Q+KALAHAFSFPAVE+IVYSTCSIHQ+ENEDVVQS+LPLAES GFQL+TPFP+WQRRGLP
Sbjct: 361 QRKALAHAFSFPAVEKIVYSTCSIHQIENEDVVQSVLPLAESRGFQLDTPFPRWQRRGLP 420

Query: 460 VFAGANHLLRTDPVEDKEGFFIALFKKKQNTSHPSSRISDRDKSSRNRGGNIRNSWPVAC 519
           VFAGANHLLRTDPVEDKEGFFIALFKKKQNT++P  R S+RDKSSR RGG++ NSW VAC
Sbjct: 421 VFAGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSSNRDKSSRYRGGDVCNSWAVAC 480

Query: 520 PPTKISKLWL--YTLSSRTRWRKNH 542
           PPTKISKLWL  Y LS+RTRWRKNH
Sbjct: 481 PPTKISKLWLYQYALSTRTRWRKNH 505

BLAST of CmUC05G099120 vs. NCBI nr
Match: XP_022135170.1 (probable 28S rRNA (cytosine-C(5))-methyltransferase [Momordica charantia])

HSP 1 Score: 892.5 bits (2305), Expect = 1.8e-255
Identity = 453/512 (88.48%), Postives = 484/512 (94.53%), Query Frame = 0

Query: 40  MGRVNNRTNASASGTAGKSENRRMSNADRSAYFARREAAKILRRVLEGDAQRRAVASIKS 99
           MGRVNNRTNASA GTAGKS+ RR+SNA RSAYFARREAAKILRRVLEGDAQRRAVASIKS
Sbjct: 1   MGRVNNRTNASAPGTAGKSDIRRLSNAARSAYFARREAAKILRRVLEGDAQRRAVASIKS 60

Query: 100 LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 159
           LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK
Sbjct: 61  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 120

Query: 160 THLAGDAEKFLMRQQSALQSVVTQILSKKKANNIEDLIAHGDDPGVLRPRFIRVNTLKMD 219
             LAGDAEKFLMRQQS LQSVVTQ+LSK+KA++IEDLI  G +PGV RPRFIRVNTLKMD
Sbjct: 121 APLAGDAEKFLMRQQSVLQSVVTQLLSKRKASSIEDLIVRGKNPGVSRPRFIRVNTLKMD 180

Query: 220 VHTAIKELGKRYAVQKDDMVPELLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDPK 279
           VH AI ELGKRYAV KDDMVP+LLVLPPGSDLHDHPLVA+GSIFMQGKASSMVAVALDPK
Sbjct: 181 VHAAISELGKRYAVHKDDMVPDLLVLPPGSDLHDHPLVANGSIFMQGKASSMVAVALDPK 240

Query: 280 PGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNENRVRRLQDTIKLSGASNIEVLHGD 339
           PGWEVLDACSAPGNKTVHLASLMNGKGR+IACELNE+RVRRLQDTIKLSGASNI+VLHGD
Sbjct: 241 PGWEVLDACSAPGNKTVHLASLMNGKGRIIACELNEDRVRRLQDTIKLSGASNIQVLHGD 300

Query: 340 FLNLNPEDPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGKTSED-DLERLNKLAAF 399
           FLNLNP+DPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGKTSED DLERLNKLAAF
Sbjct: 301 FLNLNPKDPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGKTSEDVDLERLNKLAAF 360

Query: 400 QKKALAHAFSFPAVERIVYSTCSIHQVENEDVVQSLLPLAESYGFQLETPFPQWQRRGLP 459
           Q+KALAHAFSFPAVERIVYSTCSIHQ+ENEDVVQS+LPLAES+GF+LETPFP+WQRRGLP
Sbjct: 361 QRKALAHAFSFPAVERIVYSTCSIHQIENEDVVQSILPLAESHGFELETPFPRWQRRGLP 420

Query: 460 VFAGANHLLRTDPVEDKEGFFIALFKKKQNTSHPSSRISDRDKSSRNRGGNIRNSWPVAC 519
           VF GANHLLRTDP+EDKEGFFIALFK+KQ+T+H   + S+RDKSSRNRGGNIR+ W VAC
Sbjct: 421 VFVGANHLLRTDPIEDKEGFFIALFKRKQDTTHSFPQTSNRDKSSRNRGGNIRDHWAVAC 480

Query: 520 PPTKISKLWLYTLSSRTRWRKNHTSSETRTRT 551
           PPT++ K+WLYT S RTRWRKN T  ++ TRT
Sbjct: 481 PPTRMFKMWLYTHSLRTRWRKNCTPPKSITRT 512

BLAST of CmUC05G099120 vs. ExPASy Swiss-Prot
Match: Q8GYE8 (25S rRNA (cytosine-C(5))-methyltransferase NSUN5 OS=Arabidopsis thaliana OX=3702 GN=NSUN5 PE=1 SV=1)

HSP 1 Score: 603.2 bits (1554), Expect = 2.9e-171
Identity = 309/472 (65.47%), Postives = 374/472 (79.24%), Query Frame = 0

Query: 61  RRMSNADRSAYFARREAAKILRRVLEGDAQRRAVASIKSLVYAPSVRNKKGTFALVCKTL 120
           ++ S+ +RSA +ARREAA ILR VL GDA+RRAVASIKSLV +PSVRNK+GTFALVC+TL
Sbjct: 86  QKFSSLERSALYARREAANILRTVLRGDAERRAVASIKSLVLSPSVRNKRGTFALVCETL 145

Query: 121 KYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQKTHLAGDAEKFLMRQQSALQSV 180
           KYL VIKDVL+ A++L++KWKRQ+ L++++ YDIL G+ T   GDAEKFLMR + AL S 
Sbjct: 146 KYLTVIKDVLDIANVLNSKWKRQEPLVFIVCYDILFGKDTPSIGDAEKFLMRHKEALLSG 205

Query: 181 VTQILSKKKANNIEDLIAHGDDPGVLRPRFIRVNTLKMDVHTAIKELGKRYAVQKDDMVP 240
           +  +L +KK ++++ L+      G L+PR++RVNTLKMDV +A++EL K Y VQKD+ VP
Sbjct: 206 LATLLVRKKVDSVDQLLG-SKLTGHLKPRYVRVNTLKMDVDSAVQELEKHYTVQKDETVP 265

Query: 241 ELLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDPKPGWEVLDACSAPGNKTVHLAS 300
           +LLVLPPGSDLH H LVA+G IF+QGKASSMVA AL P+ GWEVLDACSAPGNKT+HLA+
Sbjct: 266 DLLVLPPGSDLHAHRLVANGRIFLQGKASSMVAAALQPQAGWEVLDACSAPGNKTIHLAA 325

Query: 301 LMNGKGRVIACELNENRVRRLQDTIKLSGASNIEVLHGDFLNLNPEDPSLSKVRAILLDP 360
           LM G+G++IACELNE RV+RL+ TIKLSGASNIEV HGDFL LNP+DPS +K+RAILLDP
Sbjct: 326 LMEGQGKIIACELNEERVKRLEHTIKLSGASNIEVCHGDFLGLNPKDPSFAKIRAILLDP 385

Query: 361 SCSGSGTAAARLDHLLPSHVEGKTSEDDLERLNKLAAFQKKALAHAFSFPAVERIVYSTC 420
           SCSGSGT   RLDHLLPSH E      D  RL+KLA FQKKALAHA SFP VER+VYSTC
Sbjct: 386 SCSGSGTITDRLDHLLPSHSEDNNMNYDSMRLHKLAVFQKKALAHALSFPKVERVVYSTC 445

Query: 421 SIHQVENEDVVQSLLPLAESYGFQLETPFPQWQRRGLPVFAGANHLLRTDPVEDKEGFFI 480
           SI+Q+ENEDVV S+LPLA S GF+L TPFPQWQRRGLPVFAG+ HLLR DPVEDKEGFFI
Sbjct: 446 SIYQIENEDVVSSVLPLASSLGFKLATPFPQWQRRGLPVFAGSEHLLRMDPVEDKEGFFI 505

Query: 481 ALFKKKQNTSHP-SSRISDRDKSSRNRGGNIRNSWPVACPPTKISKLWLYTL 532
           ALF +     +P SS + DR    R +   ++   P  CP  K+ + W  TL
Sbjct: 506 ALFVRANKLDNPKSSELPDRVCRRRPKERTMQ-LHPYLCP--KMFRAWSGTL 553

BLAST of CmUC05G099120 vs. ExPASy Swiss-Prot
Match: Q8K4F6 (28S rRNA (cytosine-C(5))-methyltransferase OS=Mus musculus OX=10090 GN=Nsun5 PE=1 SV=2)

HSP 1 Score: 271.9 bits (694), Expect = 1.5e-71
Identity = 169/440 (38.41%), Postives = 246/440 (55.91%), Query Frame = 0

Query: 84  VLEGDAQRRAVASIKSLVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQ 143
           VL G   R+   S+K LVY+ + +N K  +ALVC+T +Y  V+  V+ +A LL  + K +
Sbjct: 10  VLAGVESRQ--GSLKGLVYSSNFQNLKQLYALVCETQRYSAVLDAVIASAGLLRAEKKLR 69

Query: 144 KELIYVIMYDILLGQKTHLAGDAEKFLM-RQQSALQSVVTQILSKKKANNIEDLIAHGDD 203
             L  V++Y++LLG+     G   K L+ R Q+ L++ + ++   +  +  EDL+     
Sbjct: 70  PHLAKVLVYELLLGKGFRGGGGRWKALLGRHQARLKAELARLKVHRGVSRNEDLLQESSR 129

Query: 204 PGVLR--PRFIRVNTLKMDVHTAI---KELGKRYAVQK----------------DDMVPE 263
           PG     PRF+RVNTLK     AI   K  G  Y  +                 D ++PE
Sbjct: 130 PGQAYQVPRFVRVNTLKTRPEDAIDYFKRQGFSYQGRASSLEDLRALKGQHFLLDPLLPE 189

Query: 264 LLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDPKPGWEVLDACSAPGNKTVHLASL 323
           LLV P  +DLH+HPL   G + +Q KAS + A+ L P PG  V+DAC+APGNKT ++A+L
Sbjct: 190 LLVFPAQTDLHEHPLYRAGHLILQDKASCLPAMLLSPPPGSHVIDACAAPGNKTSYIAAL 249

Query: 324 MNGKGRVIACELNENRVRRLQDTIKLSGASNIEVLHGDFLNLNPEDPSLSKVRAILLDPS 383
           +  +G++ A + +  R+  +   +  +G S  E+   DFL ++P D   S+V+ ILLDPS
Sbjct: 250 LKNQGKIFAFDQDAKRLAAMATLVARAGVSCCELAEKDFLTVSPSDQRYSQVQYILLDPS 309

Query: 384 CSGSGTAAARLDHLLPSHVEGKTSEDDLERLNKLAAFQKKALAHAFSFPAVERIVYSTCS 443
           CSGSG  + +L+     H EG  S+   ERL  LA FQ++AL HA  FP+++R+VYSTCS
Sbjct: 310 CSGSGMLSRQLE----EHGEGTPSK---ERLQALAGFQQRALCHALRFPSLQRLVYSTCS 369

Query: 444 IHQVENEDVVQSLLPLAESYGFQLETPFPQWQRRGLPVFAGANHLLRTDPVED-KEGFFI 501
           + Q ENEDVVQ  L    S  F+L    P W  RGL  F G+ H LR  P      GFFI
Sbjct: 370 LCQEENEDVVQEALQ-HNSGTFRLAPVLPTWPHRGLSTFPGSEHCLRASPETTLTGGFFI 429

BLAST of CmUC05G099120 vs. ExPASy Swiss-Prot
Match: Q96P11 (28S rRNA (cytosine-C(5))-methyltransferase OS=Homo sapiens OX=9606 GN=NSUN5 PE=1 SV=2)

HSP 1 Score: 271.2 bits (692), Expect = 2.6e-71
Identity = 165/425 (38.82%), Postives = 238/425 (56.00%), Query Frame = 0

Query: 84  VLEGDAQRRAVASIKSLVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQ 143
           VL G   R+   SIK LVY+ + +N K  +ALVC+T +Y  V+  V+ +A LL  + K +
Sbjct: 10  VLAGVESRQ--GSIKGLVYSSNFQNVKQLYALVCETQRYSAVLDAVIASAGLLRAEKKLR 69

Query: 144 KELIYVIMYDILLGQKTHLAGDAEKFLM-RQQSALQSVVTQILSKKKANNIEDLIAHGDD 203
             L  V++Y++LLG+     G   K L+ R Q+ L++ + ++   +  +  EDL+  G  
Sbjct: 70  PHLAKVLVYELLLGKGFRGGGGRWKALLGRHQARLKAELARLKVHRGVSRNEDLLEVGSR 129

Query: 204 PGVLR--PRFIRVNTLKM---DVHTAIKELGKRYAVQK----------------DDMVPE 263
           PG     PRF+RVNTLK    DV    K  G  Y  +                 D ++PE
Sbjct: 130 PGPASQLPRFVRVNTLKTCSDDVVDYFKRQGFSYQGRASSLDDLRALKGKHFLLDPLMPE 189

Query: 264 LLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDPKPGWEVLDACSAPGNKTVHLASL 323
           LLV P  +DLH+HPL   G + +Q +AS + A+ LDP PG  V+DAC+APGNKT HLA+L
Sbjct: 190 LLVFPAQTDLHEHPLYRAGHLILQDRASCLPAMLLDPPPGSHVIDACAAPGNKTSHLAAL 249

Query: 324 MNGKGRVIACELNENRVRRLQDTIKLSGASNIEVLHGDFLNLNPEDPSLSKVRAILLDPS 383
           +  +G++ A +L+  R+  +   +  +G S  E+   DFL ++P DP   +V  ILLDPS
Sbjct: 250 LKNQGKIFAFDLDAKRLASMATLLARAGVSCCELAEEDFLAVSPSDPRYHEVHYILLDPS 309

Query: 384 CSGSGTAAARLDHLLPSHVEGKTSEDDLERLNKLAAFQKKALAHAFSFPAVERIVYSTCS 443
           CSGSG  + +L+       E         RL+ LA FQ++AL HA +FP+++R+VYSTCS
Sbjct: 310 CSGSGMPSRQLE-------EPGAGTPSPVRLHALAGFQQRALCHALTFPSLQRLVYSTCS 369

Query: 444 IHQVENEDVVQSLLPLAESYGFQLETPFPQWQRRGLPVFAGANHLLRTDPVED-KEGFFI 486
           + Q ENEDVV+  L       F+L    P W  RGL  F GA H LR  P      GFF+
Sbjct: 370 LCQEENEDVVRDALQ-QNPGAFRLAPALPAWPHRGLSTFPGAEHCLRASPETTLSSGFFV 424

BLAST of CmUC05G099120 vs. ExPASy Swiss-Prot
Match: O14039 (25S rRNA (cytosine-C(5))-methyltransferase rcm1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rcm1 PE=3 SV=3)

HSP 1 Score: 206.8 bits (525), Expect = 6.1e-52
Identity = 141/435 (32.41%), Postives = 227/435 (52.18%), Query Frame = 0

Query: 96  SIKSLVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDIL 155
           SIK L +     + K T+ALVC+TLKY PV+ +++  ++LL  + K ++ L  V+++D+L
Sbjct: 20  SIKQLAFNSKKHDPKRTYALVCETLKYKPVLDEIIARSELLVLEKKLKENLARVLVHDLL 79

Query: 156 LGQK--THLAGDAEKFLMRQQSALQSVVTQILSKKKANNIEDLIAHGDDPGVLRPRFIRV 215
           + ++  +   G  ++ ++R ++ L +   ++  KK   + E+L        V  PR++R+
Sbjct: 80  MSKRGLSISNGPIKECILRHKTRLNAEFVKLKVKKGVKSHEELALKNP---VSLPRWLRI 139

Query: 216 NTLK-----------MDVHTAIKELG--KRYAVQKDDMVPELLVLPPGSDLHDHPLVADG 275
           NT+K           +D  ++I+ELG  K Y    DD V  L+ + P   + ++ L  +G
Sbjct: 140 NTIKSTKDEVLQGLGLDKVSSIEELGPDKFYI---DDCVENLIAIDPSFPIVENSLYKEG 199

Query: 276 SIFMQGKASSMVAVALDPKPG--WEVLDACSAPGNKTVHLASLMNGKGRVIACELNENRV 335
            + +Q KAS   A  L    G   +++D C+APGNKT HLA+    K  + A E +  RV
Sbjct: 200 KVIIQDKASCFPAAVLAGLTGHVGDIIDGCAAPGNKTTHLAACF-PKSHIFAFERDAKRV 259

Query: 336 RRLQDTIKLSGASNIEVLHGDFLNLNPEDPSLSKVRAILLDPSCSGSGTAAARLDHLLPS 395
           + L+  + +SGA+N+ + H DF   +P+      V  ILLDPSCSGSG   +R D+LL +
Sbjct: 260 QTLRKMVGISGANNVTIEHQDFTLTDPKSDLYRNVTHILLDPSCSGSG-IVSRQDYLLGN 319

Query: 396 HVEGKTSEDDLERLNKLAAFQKKALAHAFSFPAVERIVYSTCSIHQVENEDVVQSLLPLA 455
               +   +D ERL  L +FQ   L HA  FP    + YSTCS+H++ENE VV  +L   
Sbjct: 320 E---QDVTEDTERLENLCSFQSTILKHALQFPNCRHVTYSTCSVHRLENEQVVCEVLSQE 379

Query: 456 ESYGF-QLETPFPQWQRRGLPVFAG----ANHLLRTDP-VEDKEGFFIA-LFKKKQNTSH 507
             +    L    P W+ RG+P +      A  ++R  P      GFF+A L+  ++    
Sbjct: 380 PDWKCNSLTKTLPNWKTRGIPEYCAQPSMAEGMIRCKPGAGGTIGFFVANLYHPQREQET 439

BLAST of CmUC05G099120 vs. ExPASy Swiss-Prot
Match: Q9NAA7 (26S rRNA (cytosine-C(5))-methyltransferase nsun-5 OS=Caenorhabditis elegans OX=6239 GN=nsun-5 PE=1 SV=2)

HSP 1 Score: 196.1 bits (497), Expect = 1.1e-48
Identity = 137/447 (30.65%), Postives = 223/447 (49.89%), Query Frame = 0

Query: 76  EAAKILRRVLEGDAQRRAVASIKSLVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADL 135
           E A+I+R VL  +       S+++ VY  S +NKK    L C++LK+ PV  ++L+  +L
Sbjct: 9   EVAEIIRCVLAKE------KSVRNAVYGSSYKNKKALLRLSCESLKFRPVFDEILQDKEL 68

Query: 136 LSNKWKR----QKELIYVIMYDILLGQ-KTHLAGDAEKFLMRQQSALQSVVTQILSKKKA 195
            S K         EL+YV+MY+ L+G   T  + + +  + R+   ++ V  +   + + 
Sbjct: 69  KSMKRDANIGGSVELLYVLMYETLVGSGLTRCSQELKSVISRRIQRIKEV--EHAMQDEG 128

Query: 196 NNIEDLIAHGDDPGVLR-PRFIRVNTLKMDVHTAIKELG--------------------- 255
             I+ +    D    ++ PR+ R+NTLK     A+K L                      
Sbjct: 129 RGIKAMKEADDGMKKIQIPRYARINTLKWTADEAMKTLETEKWKILGTLKPENFAEMVTK 188

Query: 256 -KRYAVQKDDMVPELLVLPPG-SDLHDHPLVADGSIFMQGKASSMVAVALDPKPGWEVLD 315
            K   V  D  V  L++  P   + +++ +V    + +Q KAS + A  L+P+PG +V D
Sbjct: 189 MKDDEVYVDPHVENLIIFAPNIQNFYEYWMVEQRYLILQDKASCLPAFLLNPRPGSQVFD 248

Query: 316 ACSAPGNKTVHLASLMNGKGRVIACELNENRVRRLQDTIKLSGASNIEVLHGDFLNLNPE 375
            C+APG KT H A++M  +G+V A +   +RV  ++  +  S  +      GDFL  +  
Sbjct: 249 TCAAPGMKTSHAAAIMENQGKVWAMDRAADRVATMKQLLDASKVAIASSFCGDFLKTDVT 308

Query: 376 DPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGKTSEDDLERLNKLAAFQKKALAHA 435
           D   SKV+  ++DP CSGSG    R+D +   + E        ERL KL   Q   L HA
Sbjct: 309 DKKFSKVKFAIVDPPCSGSG-IVKRMDEITGGNAE-------KERLEKLKNLQAMILKHA 368

Query: 436 FSFPAVERIVYSTCSIHQVENEDVVQSLL---PLAESYGFQLETPFPQWQRRGLPVFAGA 490
              P ++R VYSTCS+H+ ENE VV  +L    + ++Y  + +   P+W  RGL  +   
Sbjct: 369 LKLPGLKRAVYSTCSVHEEENEQVVDEVLLDTYVRQNYVLK-KNVLPEWTYRGLKTYEVG 428

BLAST of CmUC05G099120 vs. ExPASy TrEMBL
Match: A0A0A0L2U2 (SAM_MT_RSMB_NOP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G646330 PE=3 SV=1)

HSP 1 Score: 897.1 bits (2317), Expect = 3.6e-257
Identity = 454/505 (89.90%), Postives = 481/505 (95.25%), Query Frame = 0

Query: 40  MGRVNNRTNASASGTAGKSENRRMSNADRSAYFARREAAKILRRVLEGDAQRRAVASIKS 99
           MGRVNNRT ASASG AGKSE RRMSNADRSAYFARREAAK+LRRVLEGDAQRRA+ASIK+
Sbjct: 26  MGRVNNRTKASASGAAGKSEMRRMSNADRSAYFARREAAKVLRRVLEGDAQRRAIASIKT 85

Query: 100 LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 159
           LVYAPSVRNKKGTFALVCKTLKYLPVIKDV+EAADLLSNKWKRQKELIYVIMYDILLGQK
Sbjct: 86  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVVEAADLLSNKWKRQKELIYVIMYDILLGQK 145

Query: 160 THLAGDAEKFLMRQQSALQSVVTQILSKKKANNIEDLIAHGDD-PGVLRPRFIRVNTLKM 219
           THLAGDAEKFLMRQQSALQS VTQ+LSKKKANNIEDLIA GDD PGV RPRF+RVNTLKM
Sbjct: 146 THLAGDAEKFLMRQQSALQSAVTQLLSKKKANNIEDLIARGDDGPGVSRPRFVRVNTLKM 205

Query: 220 DVHTAIKELGKRYAVQKDDMVPELLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDP 279
           DVHTAI+ELGKRYAVQKDDMV +LL+LPPGSDLHDHPLVADGSIF+QGKASSMVAVALDP
Sbjct: 206 DVHTAIEELGKRYAVQKDDMVSDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP 265

Query: 280 KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNENRVRRLQDTIKLSGASNIEVLHG 339
           KPGWEVLDACSAPGNKTVHLASLM+GKGRVIACELNENRV+RL+ TIKLSGASNI+VLHG
Sbjct: 266 KPGWEVLDACSAPGNKTVHLASLMHGKGRVIACELNENRVKRLRHTIKLSGASNIDVLHG 325

Query: 340 DFLNLNPEDPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGKTSEDDLERLNKLAAF 399
           DFLNLNP+DPSLSKVRAILLDPSCSGSGTAA RLDHLLPSH EG  S DDLERLNKLAAF
Sbjct: 326 DFLNLNPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHAEGTISGDDLERLNKLAAF 385

Query: 400 QKKALAHAFSFPAVERIVYSTCSIHQVENEDVVQSLLPLAESYGFQLETPFPQWQRRGLP 459
           Q+KALAHAFSFPAVE+IVYSTCSIHQ+ENEDVVQS+LPLAES GFQL+TPFP+WQRRGLP
Sbjct: 386 QRKALAHAFSFPAVEKIVYSTCSIHQIENEDVVQSVLPLAESRGFQLDTPFPRWQRRGLP 445

Query: 460 VFAGANHLLRTDPVEDKEGFFIALFKKKQNTSHPSSRISDRDKSSRNRGGNIRNSWPVAC 519
           VFAGANHLLRTDPVEDKEGFFIALFKKKQNT++P  R S+RDKSSR RGG++ NSW VAC
Sbjct: 446 VFAGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSSNRDKSSRYRGGDVCNSWAVAC 505

Query: 520 PPTKISKLWL--YTLSSRTRWRKNH 542
           PPTKISKLWL  Y LS+RTRWRKNH
Sbjct: 506 PPTKISKLWLYQYALSTRTRWRKNH 530

BLAST of CmUC05G099120 vs. ExPASy TrEMBL
Match: A0A6J1C0F2 (probable 28S rRNA (Cytosine-C(5))-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111007201 PE=3 SV=1)

HSP 1 Score: 892.5 bits (2305), Expect = 8.9e-256
Identity = 453/512 (88.48%), Postives = 484/512 (94.53%), Query Frame = 0

Query: 40  MGRVNNRTNASASGTAGKSENRRMSNADRSAYFARREAAKILRRVLEGDAQRRAVASIKS 99
           MGRVNNRTNASA GTAGKS+ RR+SNA RSAYFARREAAKILRRVLEGDAQRRAVASIKS
Sbjct: 1   MGRVNNRTNASAPGTAGKSDIRRLSNAARSAYFARREAAKILRRVLEGDAQRRAVASIKS 60

Query: 100 LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 159
           LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK
Sbjct: 61  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 120

Query: 160 THLAGDAEKFLMRQQSALQSVVTQILSKKKANNIEDLIAHGDDPGVLRPRFIRVNTLKMD 219
             LAGDAEKFLMRQQS LQSVVTQ+LSK+KA++IEDLI  G +PGV RPRFIRVNTLKMD
Sbjct: 121 APLAGDAEKFLMRQQSVLQSVVTQLLSKRKASSIEDLIVRGKNPGVSRPRFIRVNTLKMD 180

Query: 220 VHTAIKELGKRYAVQKDDMVPELLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDPK 279
           VH AI ELGKRYAV KDDMVP+LLVLPPGSDLHDHPLVA+GSIFMQGKASSMVAVALDPK
Sbjct: 181 VHAAISELGKRYAVHKDDMVPDLLVLPPGSDLHDHPLVANGSIFMQGKASSMVAVALDPK 240

Query: 280 PGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNENRVRRLQDTIKLSGASNIEVLHGD 339
           PGWEVLDACSAPGNKTVHLASLMNGKGR+IACELNE+RVRRLQDTIKLSGASNI+VLHGD
Sbjct: 241 PGWEVLDACSAPGNKTVHLASLMNGKGRIIACELNEDRVRRLQDTIKLSGASNIQVLHGD 300

Query: 340 FLNLNPEDPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGKTSED-DLERLNKLAAF 399
           FLNLNP+DPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGKTSED DLERLNKLAAF
Sbjct: 301 FLNLNPKDPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGKTSEDVDLERLNKLAAF 360

Query: 400 QKKALAHAFSFPAVERIVYSTCSIHQVENEDVVQSLLPLAESYGFQLETPFPQWQRRGLP 459
           Q+KALAHAFSFPAVERIVYSTCSIHQ+ENEDVVQS+LPLAES+GF+LETPFP+WQRRGLP
Sbjct: 361 QRKALAHAFSFPAVERIVYSTCSIHQIENEDVVQSILPLAESHGFELETPFPRWQRRGLP 420

Query: 460 VFAGANHLLRTDPVEDKEGFFIALFKKKQNTSHPSSRISDRDKSSRNRGGNIRNSWPVAC 519
           VF GANHLLRTDP+EDKEGFFIALFK+KQ+T+H   + S+RDKSSRNRGGNIR+ W VAC
Sbjct: 421 VFVGANHLLRTDPIEDKEGFFIALFKRKQDTTHSFPQTSNRDKSSRNRGGNIRDHWAVAC 480

Query: 520 PPTKISKLWLYTLSSRTRWRKNHTSSETRTRT 551
           PPT++ K+WLYT S RTRWRKN T  ++ TRT
Sbjct: 481 PPTRMFKMWLYTHSLRTRWRKNCTPPKSITRT 512

BLAST of CmUC05G099120 vs. ExPASy TrEMBL
Match: A0A6J1F919 (probable 28S rRNA (Cytosine-C(5))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111443222 PE=3 SV=1)

HSP 1 Score: 886.7 bits (2290), Expect = 4.9e-254
Identity = 454/511 (88.85%), Postives = 481/511 (94.13%), Query Frame = 0

Query: 40  MGRVNNRTNASASGTAGKSENRRMSNADRSAYFARREAAKILRRVLEGDAQRRAVASIKS 99
           MGRVN RT  S +GTAGKSE+RRMSNADRSAYFARREAAKILRRVLEGDAQRRAVASIKS
Sbjct: 1   MGRVNPRTKPSVAGTAGKSESRRMSNADRSAYFARREAAKILRRVLEGDAQRRAVASIKS 60

Query: 100 LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 159
           LVYAPSVRNKKGTFALVCKTLKYLP+IK+VLEAADLLSNKWKRQKELIYVIMYDILLGQK
Sbjct: 61  LVYAPSVRNKKGTFALVCKTLKYLPIIKEVLEAADLLSNKWKRQKELIYVIMYDILLGQK 120

Query: 160 THLAGDAEKFLMRQQSALQSVVTQILSKKKANNIEDLIAHGDDPGVLRPRFIRVNTLKMD 219
           T LAGDAEKFLMRQQSALQSVVTQ+LSK+KANNIEDLIA G +PGV RPRFIRVNTLKMD
Sbjct: 121 TPLAGDAEKFLMRQQSALQSVVTQLLSKRKANNIEDLIARGHNPGVSRPRFIRVNTLKMD 180

Query: 220 VHTAIKELGKRYAVQKDDMVPELLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDPK 279
           VHTAI+ELGKRYAVQKDDMVP+LLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDPK
Sbjct: 181 VHTAIQELGKRYAVQKDDMVPDLLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDPK 240

Query: 280 PGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNENRVRRLQDTIKLSGASNIEVLHGD 339
           PGWEVLDACSAPGNKTVHLASLMNG+GR+IACELNENRV+RLQ TIKLSGASNIEVLHGD
Sbjct: 241 PGWEVLDACSAPGNKTVHLASLMNGEGRIIACELNENRVKRLQHTIKLSGASNIEVLHGD 300

Query: 340 FLNLNPEDPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGKTSEDDLERLNKLAAFQ 399
           FL L+P+DPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGK+S DDLERL KLAAFQ
Sbjct: 301 FLALDPKDPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGKSSGDDLERLEKLAAFQ 360

Query: 400 KKALAHAFSFPAVERIVYSTCSIHQVENEDVVQSLLPLAESYGFQLETPFPQWQRRGLPV 459
           +KALAHAFSFPAVERIVYSTCS+HQVENEDVVQSLLPLAES+GFQLETPFPQWQRRG PV
Sbjct: 361 RKALAHAFSFPAVERIVYSTCSVHQVENEDVVQSLLPLAESHGFQLETPFPQWQRRGHPV 420

Query: 460 FAGANHLLRTDPVEDKEGFFIALFKKKQNTSHPSSRISDRDKSSRNRGGNIRNSWPVACP 519
           FAGANHLLRTDP+ED EGFFIALF KKQNT+HP  +IS RDKSSRN GGN+RNS  VAC 
Sbjct: 421 FAGANHLLRTDPIEDTEGFFIALF-KKQNTTHPFPQISSRDKSSRNHGGNVRNSRAVACL 480

Query: 520 PTKISKLWLYTLSSRTRWRKNHTSSETRTRT 551
           PT + K+W+Y  S RTR RK HT S+TRTR+
Sbjct: 481 PTSMFKMWMYAHSKRTRRRKIHTPSKTRTRS 510

BLAST of CmUC05G099120 vs. ExPASy TrEMBL
Match: A0A6J1IIH7 (probable 28S rRNA (Cytosine-C(5))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111477204 PE=3 SV=1)

HSP 1 Score: 885.2 bits (2286), Expect = 1.4e-253
Identity = 455/511 (89.04%), Postives = 481/511 (94.13%), Query Frame = 0

Query: 40  MGRVNNRTNASASGTAGKSENRRMSNADRSAYFARREAAKILRRVLEGDAQRRAVASIKS 99
           MGRVN RT  S +GTAGKSE+RRMSNADRSAYFARREAAKILRRVLEGDA RRAVASIKS
Sbjct: 1   MGRVNPRTKPSVAGTAGKSESRRMSNADRSAYFARREAAKILRRVLEGDAHRRAVASIKS 60

Query: 100 LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 159
           LVYAPSVRNKKGTFALVCKTLKYLP+IK+VLEAADLLSNKWKRQKELIYVIMYDILLGQK
Sbjct: 61  LVYAPSVRNKKGTFALVCKTLKYLPIIKEVLEAADLLSNKWKRQKELIYVIMYDILLGQK 120

Query: 160 THLAGDAEKFLMRQQSALQSVVTQILSKKKANNIEDLIAHGDDPGVLRPRFIRVNTLKMD 219
           T LAGDAEKFLMRQQSALQSVVTQ+LSK+KANNIEDLIA GD+PGV RPRFIRVNTLKMD
Sbjct: 121 TPLAGDAEKFLMRQQSALQSVVTQLLSKRKANNIEDLIARGDNPGVSRPRFIRVNTLKMD 180

Query: 220 VHTAIKELGKRYAVQKDDMVPELLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDPK 279
           VH AI+ELGKRYAVQKDDMVP+LLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDPK
Sbjct: 181 VHAAIQELGKRYAVQKDDMVPDLLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDPK 240

Query: 280 PGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNENRVRRLQDTIKLSGASNIEVLHGD 339
           PGWEVLDACSAPGNKTVHLASLMNGKGR+IACELNENRV+RLQ TI+LSGASNIEVLHGD
Sbjct: 241 PGWEVLDACSAPGNKTVHLASLMNGKGRIIACELNENRVKRLQHTIELSGASNIEVLHGD 300

Query: 340 FLNLNPEDPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGKTSEDDLERLNKLAAFQ 399
           FL L+P+DPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGK+S DD ERL+KLAAFQ
Sbjct: 301 FLALDPKDPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGKSSGDDHERLDKLAAFQ 360

Query: 400 KKALAHAFSFPAVERIVYSTCSIHQVENEDVVQSLLPLAESYGFQLETPFPQWQRRGLPV 459
           +KALAHAFSFPAVERIVYSTCSIHQVENEDVVQSLL LAES+GFQLETPFPQWQRRGLPV
Sbjct: 361 RKALAHAFSFPAVERIVYSTCSIHQVENEDVVQSLLHLAESHGFQLETPFPQWQRRGLPV 420

Query: 460 FAGANHLLRTDPVEDKEGFFIALFKKKQNTSHPSSRISDRDKSSRNRGGNIRNSWPVACP 519
           FAGANHLLRTDP+EDKEGFFIALF KKQNT+HP  +IS RDKSSRN GGNIRNS  VAC 
Sbjct: 421 FAGANHLLRTDPIEDKEGFFIALF-KKQNTTHPFPQISSRDKSSRNHGGNIRNSRAVACL 480

Query: 520 PTKISKLWLYTLSSRTRWRKNHTSSETRTRT 551
           PT + K+W++  S RTR RK HT SETRTR+
Sbjct: 481 PTSMFKMWMHAHSKRTRRRKIHTPSETRTRS 510

BLAST of CmUC05G099120 vs. ExPASy TrEMBL
Match: A0A5D3D9A2 (Putative 28S rRNA (Cytosine-C(5))-methyltransferase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001930 PE=3 SV=1)

HSP 1 Score: 874.8 bits (2259), Expect = 1.9e-250
Identity = 449/506 (88.74%), Postives = 474/506 (93.68%), Query Frame = 0

Query: 40  MGRVNNRTNASASGTAGKSENRRMSNADRSAYFARREAAKILRRVLEGDAQRRAVASIKS 99
           MGRVNNRT  S S  A KSENR+MSNADRSAYFARREAAKILRRVLEGDAQRRA+ASIKS
Sbjct: 1   MGRVNNRTKVSPSRAAYKSENRKMSNADRSAYFARREAAKILRRVLEGDAQRRAIASIKS 60

Query: 100 LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 159
           LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK
Sbjct: 61  LVYAPSVRNKKGTFALVCKTLKYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQK 120

Query: 160 THLAGDAEKFLMRQQSALQSVVTQILSKKKANNIEDLIAHG-DDPGVLRPRFIRVNTLKM 219
            HLAGDAEKFLMRQQSALQS VTQ+LSKKKANNIEDLI  G DDPGV RPRFIRVNTLKM
Sbjct: 121 AHLAGDAEKFLMRQQSALQSAVTQLLSKKKANNIEDLITRGDDDPGVSRPRFIRVNTLKM 180

Query: 220 DVHTAIKELGKRYAVQKDDMVPELLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDP 279
           DVHTAI+ELGKRY V KDDMVP+LL+LPPGSDLHDHPLVADGSIF+QGKASSMVAVALDP
Sbjct: 181 DVHTAIEELGKRYPVLKDDMVPDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDP 240

Query: 280 KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNENRVRRLQDTIKLSGASNIEVLHG 339
           KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNE+RV+RLQ TIKLSGAS I+VLHG
Sbjct: 241 KPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNESRVKRLQHTIKLSGASIIDVLHG 300

Query: 340 DFLNLNPEDPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGKTSEDDLERLNKLAAF 399
           DFLNL+P+DPSLSKVRAILLDPSCSGSGTAA RLDHLLPSHVEG TS DDLERLNKLAAF
Sbjct: 301 DFLNLSPKDPSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHVEGTTSGDDLERLNKLAAF 360

Query: 400 QKKALAHAFSFPAVERIVYSTCSIHQVENEDVVQSLLPLAESYGFQLETPFPQWQRRGLP 459
           Q+KALAHAFSFPAVERIVYSTCSIHQ+ENEDVVQSLLPLAES+GFQL+TPFPQWQRRGLP
Sbjct: 361 QRKALAHAFSFPAVERIVYSTCSIHQIENEDVVQSLLPLAESHGFQLDTPFPQWQRRGLP 420

Query: 460 VFAGANHLLRTDPVEDKEGFFIALFKKKQNTSHPSSRISDRDKSSRNRGGNIRNSWPVAC 519
           VFAGANHLLRTDPVEDKEGFFIALFKKKQNT++P  R  + DKSSR R G++RN+  +AC
Sbjct: 421 VFAGANHLLRTDPVEDKEGFFIALFKKKQNTTNPFPRSLNIDKSSRYRSGDVRNNRALAC 480

Query: 520 PPTKISKLWL--YTLSSRTRWRKNHT 543
           PPTKISKL L  Y LS+RTRWRKNH+
Sbjct: 481 PPTKISKLRLYQYALSTRTRWRKNHS 506

BLAST of CmUC05G099120 vs. TAIR 10
Match: AT5G26180.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 603.2 bits (1554), Expect = 2.1e-172
Identity = 309/472 (65.47%), Postives = 374/472 (79.24%), Query Frame = 0

Query: 61  RRMSNADRSAYFARREAAKILRRVLEGDAQRRAVASIKSLVYAPSVRNKKGTFALVCKTL 120
           ++ S+ +RSA +ARREAA ILR VL GDA+RRAVASIKSLV +PSVRNK+GTFALVC+TL
Sbjct: 86  QKFSSLERSALYARREAANILRTVLRGDAERRAVASIKSLVLSPSVRNKRGTFALVCETL 145

Query: 121 KYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQKTHLAGDAEKFLMRQQSALQSV 180
           KYL VIKDVL+ A++L++KWKRQ+ L++++ YDIL G+ T   GDAEKFLMR + AL S 
Sbjct: 146 KYLTVIKDVLDIANVLNSKWKRQEPLVFIVCYDILFGKDTPSIGDAEKFLMRHKEALLSG 205

Query: 181 VTQILSKKKANNIEDLIAHGDDPGVLRPRFIRVNTLKMDVHTAIKELGKRYAVQKDDMVP 240
           +  +L +KK ++++ L+      G L+PR++RVNTLKMDV +A++EL K Y VQKD+ VP
Sbjct: 206 LATLLVRKKVDSVDQLLG-SKLTGHLKPRYVRVNTLKMDVDSAVQELEKHYTVQKDETVP 265

Query: 241 ELLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDPKPGWEVLDACSAPGNKTVHLAS 300
           +LLVLPPGSDLH H LVA+G IF+QGKASSMVA AL P+ GWEVLDACSAPGNKT+HLA+
Sbjct: 266 DLLVLPPGSDLHAHRLVANGRIFLQGKASSMVAAALQPQAGWEVLDACSAPGNKTIHLAA 325

Query: 301 LMNGKGRVIACELNENRVRRLQDTIKLSGASNIEVLHGDFLNLNPEDPSLSKVRAILLDP 360
           LM G+G++IACELNE RV+RL+ TIKLSGASNIEV HGDFL LNP+DPS +K+RAILLDP
Sbjct: 326 LMEGQGKIIACELNEERVKRLEHTIKLSGASNIEVCHGDFLGLNPKDPSFAKIRAILLDP 385

Query: 361 SCSGSGTAAARLDHLLPSHVEGKTSEDDLERLNKLAAFQKKALAHAFSFPAVERIVYSTC 420
           SCSGSGT   RLDHLLPSH E      D  RL+KLA FQKKALAHA SFP VER+VYSTC
Sbjct: 386 SCSGSGTITDRLDHLLPSHSEDNNMNYDSMRLHKLAVFQKKALAHALSFPKVERVVYSTC 445

Query: 421 SIHQVENEDVVQSLLPLAESYGFQLETPFPQWQRRGLPVFAGANHLLRTDPVEDKEGFFI 480
           SI+Q+ENEDVV S+LPLA S GF+L TPFPQWQRRGLPVFAG+ HLLR DPVEDKEGFFI
Sbjct: 446 SIYQIENEDVVSSVLPLASSLGFKLATPFPQWQRRGLPVFAGSEHLLRMDPVEDKEGFFI 505

Query: 481 ALFKKKQNTSHP-SSRISDRDKSSRNRGGNIRNSWPVACPPTKISKLWLYTL 532
           ALF +     +P SS + DR    R +   ++   P  CP  K+ + W  TL
Sbjct: 506 ALFVRANKLDNPKSSELPDRVCRRRPKERTMQ-LHPYLCP--KMFRAWSGTL 553

BLAST of CmUC05G099120 vs. TAIR 10
Match: AT5G26180.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 603.2 bits (1554), Expect = 2.1e-172
Identity = 309/472 (65.47%), Postives = 374/472 (79.24%), Query Frame = 0

Query: 61  RRMSNADRSAYFARREAAKILRRVLEGDAQRRAVASIKSLVYAPSVRNKKGTFALVCKTL 120
           ++ S+ +RSA +ARREAA ILR VL GDA+RRAVASIKSLV +PSVRNK+GTFALVC+TL
Sbjct: 86  QKFSSLERSALYARREAANILRTVLRGDAERRAVASIKSLVLSPSVRNKRGTFALVCETL 145

Query: 121 KYLPVIKDVLEAADLLSNKWKRQKELIYVIMYDILLGQKTHLAGDAEKFLMRQQSALQSV 180
           KYL VIKDVL+ A++L++KWKRQ+ L++++ YDIL G+ T   GDAEKFLMR + AL S 
Sbjct: 146 KYLTVIKDVLDIANVLNSKWKRQEPLVFIVCYDILFGKDTPSIGDAEKFLMRHKEALLSG 205

Query: 181 VTQILSKKKANNIEDLIAHGDDPGVLRPRFIRVNTLKMDVHTAIKELGKRYAVQKDDMVP 240
           +  +L +KK ++++ L+      G L+PR++RVNTLKMDV +A++EL K Y VQKD+ VP
Sbjct: 206 LATLLVRKKVDSVDQLLG-SKLTGHLKPRYVRVNTLKMDVDSAVQELEKHYTVQKDETVP 265

Query: 241 ELLVLPPGSDLHDHPLVADGSIFMQGKASSMVAVALDPKPGWEVLDACSAPGNKTVHLAS 300
           +LLVLPPGSDLH H LVA+G IF+QGKASSMVA AL P+ GWEVLDACSAPGNKT+HLA+
Sbjct: 266 DLLVLPPGSDLHAHRLVANGRIFLQGKASSMVAAALQPQAGWEVLDACSAPGNKTIHLAA 325

Query: 301 LMNGKGRVIACELNENRVRRLQDTIKLSGASNIEVLHGDFLNLNPEDPSLSKVRAILLDP 360
           LM G+G++IACELNE RV+RL+ TIKLSGASNIEV HGDFL LNP+DPS +K+RAILLDP
Sbjct: 326 LMEGQGKIIACELNEERVKRLEHTIKLSGASNIEVCHGDFLGLNPKDPSFAKIRAILLDP 385

Query: 361 SCSGSGTAAARLDHLLPSHVEGKTSEDDLERLNKLAAFQKKALAHAFSFPAVERIVYSTC 420
           SCSGSGT   RLDHLLPSH E      D  RL+KLA FQKKALAHA SFP VER+VYSTC
Sbjct: 386 SCSGSGTITDRLDHLLPSHSEDNNMNYDSMRLHKLAVFQKKALAHALSFPKVERVVYSTC 445

Query: 421 SIHQVENEDVVQSLLPLAESYGFQLETPFPQWQRRGLPVFAGANHLLRTDPVEDKEGFFI 480
           SI+Q+ENEDVV S+LPLA S GF+L TPFPQWQRRGLPVFAG+ HLLR DPVEDKEGFFI
Sbjct: 446 SIYQIENEDVVSSVLPLASSLGFKLATPFPQWQRRGLPVFAGSEHLLRMDPVEDKEGFFI 505

Query: 481 ALFKKKQNTSHP-SSRISDRDKSSRNRGGNIRNSWPVACPPTKISKLWLYTL 532
           ALF +     +P SS + DR    R +   ++   P  CP  K+ + W  TL
Sbjct: 506 ALFVRANKLDNPKSSELPDRVCRRRPKERTMQ-LHPYLCP--KMFRAWSGTL 553

BLAST of CmUC05G099120 vs. TAIR 10
Match: AT5G55920.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 90.1 bits (222), Expect = 5.9e-18
Identity = 84/307 (27.36%), Postives = 124/307 (40.39%), Query Frame = 0

Query: 207 RPRFIRVNTLK---MDVHTAIKELGKRYAVQKDDMVPELLVLPPGSDLHDHPLVADGSIF 266
           RP  IR NTLK    D+   +   G             L++      +   P    G   
Sbjct: 283 RPTSIRTNTLKTRRRDLADVLLNRGVNLDPLSKWSKVGLVIYDSQVPIGATPEYLAGYYM 342

Query: 267 MQGKASSMVAVALDPKPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNENRVRRLQD 326
           +QG +S +  +AL P+    ++D  +APG KT ++A+LM   G + A E+   R++ L  
Sbjct: 343 LQGASSFLPVMALAPRENERIVDVAAAPGGKTTYIAALMKNTGLIYANEMKVPRLKSLTA 402

Query: 327 TIKLSGASNIEVLHGDFLNLNPEDPSLSKVRAILLDPSCSGSGTAAARLDHLLPSHVEGK 386
            +   G +N  V + D   L P+    + V  +LLD  CSG+G         + S  E  
Sbjct: 403 NLHRMGVTNTIVCNYDGREL-PKVLGQNTVDRVLLDAPCSGTG---------IISKDESV 462

Query: 387 TSEDDLERLNKLAAFQKKALAHAFSFPAVER-----IVYSTCSIHQVENEDVVQSLLPLA 446
                ++ + K A  QK+ L  A             IVYSTCSI   ENE V+   L   
Sbjct: 463 KITKTMDEIKKFAHLQKQLLLAAIDMVDANSKTGGYIVYSTCSIMVTENEAVIDYAL--- 522

Query: 447 ESYGFQLETPFPQWQRRGLPVFAGANHLLRTDP-------VEDKEGFFIALFKKKQNTSH 499
           +    +L T    + R+G   F         D        V + +GFF+A  KK  N   
Sbjct: 523 KKRDVKLVTCGLDFGRKGFTRFREHRFQPSLDKTRRFYPHVHNMDGFFVAKLKKMSNVKQ 576

BLAST of CmUC05G099120 vs. TAIR 10
Match: AT3G13180.1 (NOL1/NOP2/sun family protein / antitermination NusB domain-containing protein )

HSP 1 Score: 85.1 bits (209), Expect = 1.9e-16
Identity = 57/181 (31.49%), Postives = 93/181 (51.38%), Query Frame = 0

Query: 256 LVADGSIFMQGKASSMVAVALDPKPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNE 315
           L+ +G   +Q +++ ++   + P+PG  ++DAC+APG KT+ +AS + G+G + A ++NE
Sbjct: 306 LLKEGICSVQDESAGLIVSVVKPQPGERIMDACAAPGGKTLFMASCLKGQGMIYAMDVNE 365

Query: 316 NRVRRLQDTIKLSGASN-IEVLHGDFLNLNPEDPSLSKVRAILLDPSCSGSGTAAARLDH 375
            R+R L +T K       I  +H D L +  E   + +   +LLD  CSG G  + R D 
Sbjct: 366 GRLRILGETAKSHQVDGLITTIHSD-LRVFAETNEV-QYDKVLLDAPCSGLGVLSKRAD- 425

Query: 376 LLPSHVEGKTSEDDLERLNKLAAFQKKALAHAFSFPAVERIVYSTCSIHQVENEDVVQSL 435
                +      +D+  L KL      + +       V  +VYSTCSI   ENE  V++ 
Sbjct: 426 -----LRWNRKLEDMLELTKLQDELLDSASKLVKHGGV--LVYSTCSIDPEENEGRVEAF 476

BLAST of CmUC05G099120 vs. TAIR 10
Match: AT1G06560.1 (NOL1/NOP2/sun family protein )

HSP 1 Score: 64.7 bits (156), Expect = 2.7e-10
Identity = 29/67 (43.28%), Postives = 45/67 (67.16%), Query Frame = 0

Query: 257 VADGSIFMQGKASSMVAVALDPKPGWEVLDACSAPGNKTVHLASLMNGKGRVIACELNEN 316
           + +G IF+Q   S +VA ALDP+ G  +LD C+APG KT  +A LMN +G ++A + + N
Sbjct: 273 ILEGEIFLQNLPSIIVAHALDPQKGERILDMCAAPGGKTTAIAILMNDEGEIVAADRSHN 332

Query: 317 RVRRLQD 324
           +V  +Q+
Sbjct: 333 KVLVVQN 339

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038898181.12.9e-26192.2828S rRNA (cytosine-C(5))-methyltransferase-like isoform X4 [Benincasa hispida][more]
XP_038898177.13.0e-25891.6828S rRNA (cytosine-C(5))-methyltransferase-like isoform X1 [Benincasa hispida][more]
XP_004141333.24.4e-25787.57probable 28S rRNA (cytosine-C(5))-methyltransferase [Cucumis sativus][more]
KAE8649915.17.5e-25789.90hypothetical protein Csa_012918 [Cucumis sativus][more]
XP_022135170.11.8e-25588.48probable 28S rRNA (cytosine-C(5))-methyltransferase [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q8GYE82.9e-17165.4725S rRNA (cytosine-C(5))-methyltransferase NSUN5 OS=Arabidopsis thaliana OX=3702... [more]
Q8K4F61.5e-7138.4128S rRNA (cytosine-C(5))-methyltransferase OS=Mus musculus OX=10090 GN=Nsun5 PE=... [more]
Q96P112.6e-7138.8228S rRNA (cytosine-C(5))-methyltransferase OS=Homo sapiens OX=9606 GN=NSUN5 PE=1... [more]
O140396.1e-5232.4125S rRNA (cytosine-C(5))-methyltransferase rcm1 OS=Schizosaccharomyces pombe (st... [more]
Q9NAA71.1e-4830.6526S rRNA (cytosine-C(5))-methyltransferase nsun-5 OS=Caenorhabditis elegans OX=6... [more]
Match NameE-valueIdentityDescription
A0A0A0L2U23.6e-25789.90SAM_MT_RSMB_NOP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G64... [more]
A0A6J1C0F28.9e-25688.48probable 28S rRNA (Cytosine-C(5))-methyltransferase OS=Momordica charantia OX=36... [more]
A0A6J1F9194.9e-25488.85probable 28S rRNA (Cytosine-C(5))-methyltransferase OS=Cucurbita moschata OX=366... [more]
A0A6J1IIH71.4e-25389.04probable 28S rRNA (Cytosine-C(5))-methyltransferase OS=Cucurbita maxima OX=3661 ... [more]
A0A5D3D9A21.9e-25088.74Putative 28S rRNA (Cytosine-C(5))-methyltransferase isoform X1 OS=Cucumis melo v... [more]
Match NameE-valueIdentityDescription
AT5G26180.12.1e-17265.47S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G26180.22.1e-17265.47S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G55920.15.9e-1827.36S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT3G13180.11.9e-1631.49NOL1/NOP2/sun family protein / antitermination NusB domain-containing protein [more]
AT1G06560.12.7e-1043.28NOL1/NOP2/sun family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023267RNA (C5-cytosine) methyltransferasePRINTSPR02008RCMTFAMILYcoord: 468..485
score: 30.24
coord: 285..295
score: 67.26
coord: 256..270
score: 24.27
coord: 412..428
score: 46.72
coord: 354..366
score: 52.08
IPR001678SAM-dependent methyltransferase RsmB/NOP2-typePFAMPF01189Methyltr_RsmB-Fcoord: 274..484
e-value: 1.3E-26
score: 93.5
IPR001678SAM-dependent methyltransferase RsmB/NOP2-typePROSITEPS51686SAM_MT_RSMB_NOPcoord: 199..486
score: 49.716988
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 265..492
e-value: 4.2E-69
score: 234.1
NoneNo IPR availableGENE3D3.30.70.1170Sun protein; domain 3coord: 228..264
e-value: 6.3E-7
score: 31.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 491..506
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 490..515
NoneNo IPR availablePANTHERPTHR22807NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAININGcoord: 48..512
NoneNo IPR availablePANTHERPTHR22807:SF66BNAA09G04380D PROTEINcoord: 48..512
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 284..372
e-value: 5.57256E-9
score: 52.0471
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 209..486

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmUC05G099120.1CmUC05G099120.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001510 RNA methylation
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0003723 RNA binding