CmUC05G086250 (gene) Watermelon (USVL531) v1

Overview
NameCmUC05G086250
Typegene
OrganismCitrullus mucosospermus (Watermelon (USVL531) v1)
DescriptionNEDD8-activating enzyme E1 catalytic subunit
LocationCmU531Chr05: 5002646 .. 5007622 (-)
RNA-Seq ExpressionCmUC05G086250
SyntenyCmUC05G086250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGGCTCTGTCGTCCAATCTAGTCGATCGAGGGACCTCGATAAGCTCCTTCTTAGGCCCGGTAACCTCATCGGCGCCAATTTCGAGCCCGGTTCCCAAGTGGGTACTTCTTCTTCTCTCTGACATTTTGTCGAATACCTTGTGTTCAGTACATGGTCTGCAATCGTCTTACGACTTAGTTGTTTTGCATTACAGTTGAGGGATGATCTTCAACAGTATGTGAAAGTGTTGGTCATAGGAGCTGGGGGGTTGGGCTGCGAGCTCCTGAAGGACTTGGCTTTGTCGGGTTTCCGGAATTTGGAAGTCATTGACATGGATCGCATCGAAGTTACTAATCTCAATCGTCAGTTTCTCTTCAGGTTTCAACTATTTTCTTGGTTCTATATGTATCTTTCGACTTCTATTCGTAGTGTGTTTTCTTAATTTTTGAACAACCGTGGCCCGGGGGGTGGGTTTAGAACCTTTACTTTTTGTAGATTGAATACTGCGCTGTGATGACTTTTCAAATTGATCTGTATTTCTAAGTTTCATCCATTATTTGACTGATGATATGTATATATTTATGATTTGTTATTGTAAAAGCTGCACCGGTTCTAGGAATTCGACGGATCGAATGTCACATTTTACTTGACTGGAGCCTTTCTCATAGTAGGCTTCCCGTTTTGTGGACTTCTCCTTTTTGTATGTCAATTCTTGCATGTTTTTCTCGATGAAAGTCCAATTCTTACATCAAAAAGGAAAAGAAAAGAAAAAAAGATCAATGGATCGAATGTTCTTTCCTTTTAACCATTTGTACTAGAATTCTGAACCAGCCGAATAAGCATGGTGCTTCAATAGATTTTCCTGAAAGAAAGTTCTTTTCAGGATATTTTACTAATACGGTATGATTTGACCCTGGACTCCCTTGAAGAAGTCACATTTACACATGTACATTTGCACATGGCTTTACAATAGTCATTTGATTTAGTATGGCAGTCAGGAGAATAACACTAGGTAGAATTTCAGGCTTGAAGATGTTGGTAAGCCAAAAGCTGAGGTGGCTGCAAAACGTGTTATGGAGAGAGTTAGTGGTGTCAATATTCTGCCGCATTTTTGCCGGATTGAGGATAAAGAAATTGAATTTTACAACGATTTTCACATAATCGCACTTGGTCTTGATTCCATTGAAGCTCGAAGTTACATCAATTCCGTCGCTTGTAGCTTCCTTGGTATGCCATTTTTGGACTTGCTATGTTCTCCTTATGTCTCTTCACCATCTTGCCTTCATTAAATGTTAATGTTGTGTTGTCGATGAATGAGAACTTATATGTCCTTTATTTTCTCTTTGCAACATCTAGAGTATGATTCTGATGATAATCCATTAGAAGAAACTATTAAACCAATGGTAGATGGTGGAACTGAAGGCTTCAAGGGGCATGCGAGGGTAATTTTACCAGGGGTCACACCATGCTTCGAGTGCACCATCTGGCTTTTTCCACCTCAAGTAAAGTTTCCATTATGTACCCTAGCAGAAACTCCTAGAACTGCTGCTCATTGTATCGAGTATGCCCATCTAATTAAATGGGATGAGGTAGTACATTAGTTATCAGGCTTCTCGTTGATGATTGTAAATGCACCCCTTTTGGACTTACCAGCACTTTTTCTTTTAGGTTCATAGTGGAAAAGCTTTTGATCCAGATGATTCTGAACATATGAAATGGGTTTACAGTGAGGTACACAAATTCCTGCAAGATCTGGTAATTCTTTTCAATTACTGATTCTAGACTTCTAATTTATCCACCCACATGTTTAGGCTGTTAAGAGAGCTGAGCTTTTTGGCATTCCGGGAGTTACATATTCACTAACTCAGGTACCTATTAGTCTGGGCATTTTCACATCACTGAAGTGATGATTGTTTTTGTTTTTATTTTTTTATCTGAACTTGGGATTGTTCTTTTAGCTACATATTATCTGTTTGCATTTTTTGTTAACTACTCACTCTTTGTGATTTACAAATGCCCCTACTGATTTACATTTTGTAAGTGATTTTTAAGGGTGTTGTTAAGAATATCATACCTGCTATTGCTTCCACCAATGCAATTATATCAGCTGCATGTGCACTGGAAACGTTGAAGATTGTATCAGGATGCAGCAAAACTCTATCCAACTATTTAACGTGCGTACTGTTTCCTTTAACTATGTGACAATTCCCTTTCATTTTAACGTAGTATGGGTGCAATAATAATTTTGTTGTGCAACTTAATATTGTTTTTCTTTATTTCTTACTCCTAATTGTATAGGTACATAACATTTCAGACTGCAATGAGACATTTGGATTTATTATTATGGGTTCTTTGGGGTAGCGGAATAATATCTTGTTTTGAGGGTTGGAGATGAATCCTAATTATATTTGGTCCCTTCTTAGATTTCATGTTTTCCTATAGGCTTTGATTTCGAAGACCATATGTAATTATTTTCAAGACACTATTTTGTATAGCTGAAGCCTCTTCCTTTAGTAGGCTCCTTTTTTAACATTGTGCCATTATTTCTTATAGCCTTTTTAACATTGTGCTATTATTTCCTTTTATTTGTGGCCTTAATTTCCAAGTGCATTGAATTTAATGCCAAGTGATCTTTTTCCAGATATAATGGTGCAGAAGGTCTTCACACTAAGGTGACCGAGTTTGTAAAAGATAAGGACTGTCTTGTTTGTGGTCCAGGCGTTCTTATTGAGCTGGATTCTTCAATTACTTTACAAAAGGTCAGTTATTTAGCTTATGCCACCTCTAGAAACTACATGTTATATGCAGTATTACAGACGCATCACTGTATTCATCTTGTATTCGGTTGTTGCTGTGTTAGTAAATTTCAGATTTTGAGAAATCTTGTTAATCCTTCATGACGGTTCTTCGCAATTTGAATTTGTATGAAACAGTTCATTGACCTGTTAGAAGATCATCCGAAGCTCTTGTTATCAAAGGCAAGTGTCACGCATCGAGGGAAGAATCTGTACATGCAGGCACCTCCTGTACTTGAAGAGATGACCAGATCAAATTTGGGTGTTCCACTTTTTGACTTGATGGGTAAAGTTTTGAAAGACGTAGTCCATGTGACTGGTGTAGCTTCTAAGAATGATAAGAAAACTTCCTGTCTTCGAAAACTACGTGTGGCTTTTAAGGGGGTTGACGGTGTTACGGATATGGATACTGCAAGTGGGGCTTAATATTACATTGCATTTTTATCTACAAATTTGGGTCAGATGGCTCTCTCCCCCCTCTGACACGGAAATTAATGGAAAAAGGTTGATGTGGGGTCTGATCTATTCCCATTTTGTATAATTTTTTTTTTCTTGTTCCAAGATTCCTGAAAAGAACAATAGAGCTGTGAATTTGTCGCCATAGAAGACTGATGTATTGCACAATGATTTACTTCTTTCATCCCTCTACTTCAAGCAACCATGTGAATATTAACTGCTAACCATGATTATGACTTAACACAGTGGATATTTTTTAAGATTTCCTTGTCATTTGTATCAGCTTCAGCTTGGAAAAATAGGCAACAAGTTTGTTGCTGTTGTATATTGAACAATGATGCCAATCCTTTGGGAAGATTGTTTTTTATCTGGTTAAGTAGAAGAAAGAAAGGAAGACACAGTATTGTTTTGTCTTTGGCCTCTCTCATTCTTCCTCCTGTTTTATACAACATGTTTGTAAATGATACGTTACTCTTCATTTAGGTTATATCTTTTAATTCTATGAGTTGTGTTTTTTTAAAATGAATGCATCTTTTCTCTTTTTAACTAACTTGAAGAAGAGACTTGACCATCCAAATGATCAAGAATTAGTCTATAAATGTCTCTTCTTCAAATTAGAACTTCACCTTTTTTATAAGCACTTTTCTCCCGTAAAAATTTGTTTGCTTTTAAATTTCACTGGGTTTGAAACTTACTTTTGAGAGACAAGTACTCTTGAAACTTAGACACTAGAGTGAGTAAAATTGTCTTAAGAAGAGAAGGTGAATTTTTTTTAGCTCAAATTTTCTTTTGTATAGGAGGTTATTGGTTTTCTAGTTTTATGTGATATTATTCTTATAACAACATATTTACAATGAATTCTCTCTCCAGCTGTCGATCCCAAAATCTGATTGGTTTGCTTGCTGAAGACAGGATATGATCTTGGAGAAACCAAGAGATTTACTGATGCTGAACATTTAATGCTATAGTAGAAAGAAAGAACTCTTTGATCTAACATTTACCTGTAGAAGCCTCCAATTCACAAAGAAAGTTGGGCAGGAAACATGGAGGGGCCCTCTACCAGCTAGCAAACCGAGTGAAATTGTGGAACCCTGCTATCCCCATGATTAATAGTTTAGTACTTTGCACATTTGGGTTCACTGTAAAGTGGATGAAAATAGTGATATGGATTTTAATGAGCCTTTGGAATATGTCATGTTCATTCGATTATCTCTGAAGTTGACTGTAGAGGAGAGTTGATTCCCCTTTTTCTTTTTCTGTGATAATATTTGTTTGTGGATTTCCTGAAATTATTGAACATAGTCTAGGAAAGGAGGTATTTGGCTTAAAGATAAGATTTTGTATATTTCGACCATAGAAGGATTAGTTTCATGTTTGTATAGTGGCTTTATTTTCTATTATTTGCATCTCTCTCAATCGTTGTTTTATTAAAATTATGAGCTAAACATCTCTTCATCGTTGTACATGTTCCTTGACACTACAAATGCAAGGGGCCCTGCGAAAGAGATGTCCACCGACTCCTAGATCTAATGGTCTTAAACAACACACAGATGCACGGATGAGTACCTATGACAGTTAGCTTAGCTAGTTCAAGTTGAGAGCCAGGTAGATCTTCTCTCATTTACGAGAATACAGATCATTTATTGCTTTTTTGAATACTTGTCAAAGATTCTCGATCAGATCTAAGTCGTCATATGAAACATGCTAATGAGATATGCTTTGATGAACAGAGAATCCATTAA

mRNA sequence

ATGGCGGGCTCTGTCGTCCAATCTAGTCGATCGAGGGACCTCGATAAGCTCCTTCTTAGGCCCGGTAACCTCATCGGCGCCAATTTCGAGCCCGGTTCCCAATTGAGGGATGATCTTCAACAGTATGTGAAAGTGTTGGTCATAGGAGCTGGGGGGTTGGGCTGCGAGCTCCTGAAGGACTTGGCTTTGTCGGGTTTCCGGAATTTGGAAGTCATTGACATGGATCGCATCGAAGTTACTAATCTCAATCGTCAGTTTCTCTTCAGGCTTGAAGATGTTGGTAAGCCAAAAGCTGAGGTGGCTGCAAAACGTGTTATGGAGAGAGTTAGTGGTGTCAATATTCTGCCGCATTTTTGCCGGATTGAGGATAAAGAAATTGAATTTTACAACGATTTTCACATAATCGCACTTGGTCTTGATTCCATTGAAGCTCGAAGTTACATCAATTCCGTCGCTTGTAGCTTCCTTGAGTATGATTCTGATGATAATCCATTAGAAGAAACTATTAAACCAATGGTAGATGGTGGAACTGAAGGCTTCAAGGGGCATGCGAGGGTAATTTTACCAGGGGTCACACCATGCTTCGAGTGCACCATCTGGCTTTTTCCACCTCAAGTAAAGTTTCCATTATGTACCCTAGCAGAAACTCCTAGAACTGCTGCTCATTGTATCGAGTATGCCCATCTAATTAAATGGGATGAGGTTCATAGTGGAAAAGCTTTTGATCCAGATGATTCTGAACATATGAAATGGGTTTACAGTGAGGCTGTTAAGAGAGCTGAGCTTTTTGGCATTCCGGGAGTTACATATTCACTAACTCAGGGTGTTGTTAAGAATATCATACCTGCTATTGCTTCCACCAATGCAATTATATCAGCTGCATGTGCACTGGAAACGTTGAAGATTGTATCAGGATGCAGCAAAACTCTATCCAACTATTTAACATATAATGGTGCAGAAGGTCTTCACACTAAGGTGACCGAGTTTGTAAAAGATAAGGACTGTCTTGTTTGTGGTCCAGGCGTTCTTATTGAGCTGGATTCTTCAATTACTTTACAAAAGTTCATTGACCTGTTAGAAGATCATCCGAAGCTCTTGTTATCAAAGGCAAGTGTCACGCATCGAGGGAAGAATCTGTACATGCAGGCACCTCCTGTACTTGAAGAGATGACCAGATCAAATTTGGGTGTTCCACTTTTTGACTTGATGGGTAAAGTTTTGAAAGACGTAGTCCATGTGACTGGTGTAGCTTCTAAGAATGATAAGAAAACTTCCTGTCTTCGAAAACTACGTGTGGCTTTTAAGGGGGTTGACGGTGTTACGGATATGGATACTGCAATTCAAGTTGAGAGCCAGAGAATCCATTAA

Coding sequence (CDS)

ATGGCGGGCTCTGTCGTCCAATCTAGTCGATCGAGGGACCTCGATAAGCTCCTTCTTAGGCCCGGTAACCTCATCGGCGCCAATTTCGAGCCCGGTTCCCAATTGAGGGATGATCTTCAACAGTATGTGAAAGTGTTGGTCATAGGAGCTGGGGGGTTGGGCTGCGAGCTCCTGAAGGACTTGGCTTTGTCGGGTTTCCGGAATTTGGAAGTCATTGACATGGATCGCATCGAAGTTACTAATCTCAATCGTCAGTTTCTCTTCAGGCTTGAAGATGTTGGTAAGCCAAAAGCTGAGGTGGCTGCAAAACGTGTTATGGAGAGAGTTAGTGGTGTCAATATTCTGCCGCATTTTTGCCGGATTGAGGATAAAGAAATTGAATTTTACAACGATTTTCACATAATCGCACTTGGTCTTGATTCCATTGAAGCTCGAAGTTACATCAATTCCGTCGCTTGTAGCTTCCTTGAGTATGATTCTGATGATAATCCATTAGAAGAAACTATTAAACCAATGGTAGATGGTGGAACTGAAGGCTTCAAGGGGCATGCGAGGGTAATTTTACCAGGGGTCACACCATGCTTCGAGTGCACCATCTGGCTTTTTCCACCTCAAGTAAAGTTTCCATTATGTACCCTAGCAGAAACTCCTAGAACTGCTGCTCATTGTATCGAGTATGCCCATCTAATTAAATGGGATGAGGTTCATAGTGGAAAAGCTTTTGATCCAGATGATTCTGAACATATGAAATGGGTTTACAGTGAGGCTGTTAAGAGAGCTGAGCTTTTTGGCATTCCGGGAGTTACATATTCACTAACTCAGGGTGTTGTTAAGAATATCATACCTGCTATTGCTTCCACCAATGCAATTATATCAGCTGCATGTGCACTGGAAACGTTGAAGATTGTATCAGGATGCAGCAAAACTCTATCCAACTATTTAACATATAATGGTGCAGAAGGTCTTCACACTAAGGTGACCGAGTTTGTAAAAGATAAGGACTGTCTTGTTTGTGGTCCAGGCGTTCTTATTGAGCTGGATTCTTCAATTACTTTACAAAAGTTCATTGACCTGTTAGAAGATCATCCGAAGCTCTTGTTATCAAAGGCAAGTGTCACGCATCGAGGGAAGAATCTGTACATGCAGGCACCTCCTGTACTTGAAGAGATGACCAGATCAAATTTGGGTGTTCCACTTTTTGACTTGATGGGTAAAGTTTTGAAAGACGTAGTCCATGTGACTGGTGTAGCTTCTAAGAATGATAAGAAAACTTCCTGTCTTCGAAAACTACGTGTGGCTTTTAAGGGGGTTGACGGTGTTACGGATATGGATACTGCAATTCAAGTTGAGAGCCAGAGAATCCATTAA

Protein sequence

MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGVTDMDTAIQVESQRIH
Homology
BLAST of CmUC05G086250 vs. NCBI nr
Match: XP_004134149.1 (NEDD8-activating enzyme E1 catalytic subunit [Cucumis sativus] >KGN57012.1 hypothetical protein Csa_009619 [Cucumis sativus])

HSP 1 Score: 898.7 bits (2321), Expect = 2.1e-257
Identity = 444/446 (99.55%), Postives = 446/446 (100.00%), Query Frame = 0

Query: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK+
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKS 240

Query: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDDSEHMKWVYSEA+KRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420
           DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN
Sbjct: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTA 447
           DKKTSCLRKLRVAFKGVDGVTDMDTA
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTA 446

BLAST of CmUC05G086250 vs. NCBI nr
Match: XP_038897781.1 (NEDD8-activating enzyme E1 catalytic subunit [Benincasa hispida])

HSP 1 Score: 897.9 bits (2319), Expect = 3.6e-257
Identity = 443/446 (99.33%), Postives = 445/446 (99.78%), Query Frame = 0

Query: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGS+VQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSIVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNI+PHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNIVPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420
           DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKD VHVTGVASKN
Sbjct: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDAVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTA 447
           DKKTSCLRKLRVAFKGVDGVTDMDTA
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTA 446

BLAST of CmUC05G086250 vs. NCBI nr
Match: XP_008438709.1 (PREDICTED: NEDD8-activating enzyme E1 catalytic subunit [Cucumis melo])

HSP 1 Score: 897.5 bits (2318), Expect = 4.7e-257
Identity = 444/446 (99.55%), Postives = 446/446 (100.00%), Query Frame = 0

Query: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDDSEHMKWVYSEA+KRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420
           D+PKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN
Sbjct: 361 DYPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTA 447
           DKKTSCLRKLRVAFKGVDGVTDMDTA
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTA 446

BLAST of CmUC05G086250 vs. NCBI nr
Match: KAA0049385.1 (NEDD8-activating enzyme E1 catalytic subunit [Cucumis melo var. makuwa] >TYK17174.1 NEDD8-activating enzyme E1 catalytic subunit [Cucumis melo var. makuwa])

HSP 1 Score: 896.0 bits (2314), Expect = 1.4e-256
Identity = 443/446 (99.33%), Postives = 445/446 (99.78%), Query Frame = 0

Query: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDDSEHMKWVYSEA+KRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420
           D+PKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN
Sbjct: 361 DYPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTA 447
           DKKTSCLRKLRV FKGVDGVTDMDTA
Sbjct: 421 DKKTSCLRKLRVTFKGVDGVTDMDTA 446

BLAST of CmUC05G086250 vs. NCBI nr
Match: XP_022138079.1 (NEDD8-activating enzyme E1 catalytic subunit isoform X1 [Momordica charantia])

HSP 1 Score: 886.7 bits (2290), Expect = 8.4e-254
Identity = 435/449 (96.88%), Postives = 444/449 (98.89%), Query Frame = 0

Query: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLV+GAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVVGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRL+DVGKPKAEVAAKRVMERVSGVNI+PHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLQDVGKPKAEVAAKRVMERVSGVNIVPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYIN+VACSFLEYDSDD+PLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINAVACSFLEYDSDDSPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK 
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKT 240

Query: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDD  HMKWVY+EAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDPVHMKWVYNEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFV+DKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVRDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420
           DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNL VPLFDLMGKVLKDVVHVTGVASKN
Sbjct: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTAIQV 450
           DKKTSCLRKLRVAFKGVDGVTDMDTA+ +
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTAVDI 449

BLAST of CmUC05G086250 vs. ExPASy Swiss-Prot
Match: O65041 (NEDD8-activating enzyme E1 catalytic subunit OS=Arabidopsis thaliana OX=3702 GN=ECR1 PE=1 SV=2)

HSP 1 Score: 728.8 bits (1880), Expect = 3.8e-209
Identity = 355/443 (80.14%), Postives = 388/443 (87.58%), Query Frame = 0

Query: 6   VQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSG 65
           V  S++RDLDKLLLR GNL+   F PG  LRDD++ YV++LVIGAGGLGCELLKDLALSG
Sbjct: 10  VPQSKTRDLDKLLLRHGNLVDPGFVPGPGLRDDIRDYVRILVIGAGGLGCELLKDLALSG 69

Query: 66  FRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKE 125
           FRNLEVIDMDRIEVTNLNRQFLFR+EDVGKPKAEVAAKRVMERVSGV I+PHF RIEDKE
Sbjct: 70  FRNLEVIDMDRIEVTNLNRQFLFRIEDVGKPKAEVAAKRVMERVSGVEIVPHFSRIEDKE 129

Query: 126 IEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHAR 185
           IEFYNDF+IIALGLDSIEAR YIN VAC FLEY+ DD P  ETIKPMVDGGTEGFKGHAR
Sbjct: 130 IEFYNDFNIIALGLDSIEARKYINGVACGFLEYNEDDTPKRETIKPMVDGGTEGFKGHAR 189

Query: 186 VILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDD 245
           VILPGVTPCFECTI+LFPPQVKFPLCTLAETPR AAHCIEYAHLI+W+ VH GK FDPD+
Sbjct: 190 VILPGVTPCFECTIYLFPPQVKFPLCTLAETPRNAAHCIEYAHLIQWETVHRGKTFDPDE 249

Query: 246 SEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSG 305
            EHMKWVY EA++RAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVS 
Sbjct: 250 PEHMKWVYDEAIRRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSA 309

Query: 306 CSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDHPKL 365
           CSKTL NYLTYNG EGL+T+VT+F +D +CLVCGPG+LIELD+S+TL KFI++LEDHPKL
Sbjct: 310 CSKTLVNYLTYNGGEGLYTEVTKFERDTECLVCGPGILIELDTSVTLSKFIEMLEDHPKL 369

Query: 366 LLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGV-ASKNDKKT 425
           LLSKASV      LYMQAPPVLEE  R  L  PL+DLMG+V KD +HV G  A KN++K 
Sbjct: 370 LLSKASVKQGENTLYMQAPPVLEEFHRPKLSKPLYDLMGRVQKDTIHVFGQRALKNNEKE 429

Query: 426 SCLRKLRVAFKGVDGVTDMDTAI 448
           SC  K+RV FKG DGV DMDTAI
Sbjct: 430 SCTTKVRVVFKGADGVADMDTAI 452

BLAST of CmUC05G086250 vs. ExPASy Swiss-Prot
Match: Q54QG9 (NEDD8-activating enzyme E1 catalytic subunit OS=Dictyostelium discoideum OX=44689 GN=uba3 PE=1 SV=1)

HSP 1 Score: 383.3 bits (983), Expect = 3.9e-105
Identity = 201/412 (48.79%), Postives = 267/412 (64.81%), Query Frame = 0

Query: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQ----LRDDLQQYVKVLVIGAGGLGCE 60
           M  SV    R  D++K++ R G     +FEP ++    + + LQ   KVLVIGAGGLGCE
Sbjct: 4   MDTSVDLPGRWIDIEKIIKRTGPFASPSFEPDTKASPNIMNGLQNDFKVLVIGAGGLGCE 63

Query: 61  LLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILP 120
           +LK+LALSGFRN++VIDMD I+++NLNRQFLFR +DVGK KAEVAA  +  R++G N+ P
Sbjct: 64  ILKNLALSGFRNIDVIDMDTIDISNLNRQFLFRRKDVGKSKAEVAAAFINSRITGCNVTP 123

Query: 121 HFCRIEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGG 180
           H CRI+DK+ ++Y  F I+  GLDSIEAR +IN +  + +  +   +   +TI P+VDGG
Sbjct: 124 HKCRIQDKDEDYYRQFKIVIAGLDSIEARRWINGLLVNLVVVNDSGDIEPDTIIPLVDGG 183

Query: 181 TEGFKGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVH 240
           TEGFKG ARVILP ++ CFEC++  FPPQV + +CT+A TPR   HCI++A L    +  
Sbjct: 184 TEGFKGQARVILPKISSCFECSLDAFPPQVSYAICTIANTPRVPEHCIQWALLFGLQDAT 243

Query: 241 SGKAFDP-----DDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAII 300
             K FDP     D+ +HM W++  A KRAE F I GVTY LTQGV KNIIPAIASTNAII
Sbjct: 244 LEKPFDPKQFDNDNPDHMNWLFECAKKRAEKFNINGVTYKLTQGVAKNIIPAIASTNAII 303

Query: 301 SAACALETLKIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPG-VLIELDSSI 360
           +AAC  E  K  +  S  L+NY+ YNG  G++T   E+   + C VCG   V  E+D S 
Sbjct: 304 AAACCNEVFKFCTDSSGYLNNYMMYNGLNGVYTFTFEYEIKEGCAVCGTNLVTFEIDKSN 363

Query: 361 TLQKFIDLLEDHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDL 403
           TL  F++ +    +    K S+   G+NLYMQ   +L + T  NL   L +L
Sbjct: 364 TLSTFLEKITTDSRFQFKKPSLRSNGRNLYMQG--LLHQSTVPNLEKTLSEL 413

BLAST of CmUC05G086250 vs. ExPASy Swiss-Prot
Match: Q9V6U8 (Nedd8-activating enzyme E1 catalytic subunit OS=Drosophila melanogaster OX=7227 GN=Uba3 PE=1 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 5.1e-105
Identity = 200/409 (48.90%), Postives = 262/409 (64.06%), Query Frame = 0

Query: 3   GSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLA 62
           G ++QS R   L  +L R G      F   S+  + LQ   +VL+IGAGGLGCELLKDLA
Sbjct: 9   GLILQSKRFNGLRNILEREGPFCKDGFAASSENLEFLQTKCQVLIIGAGGLGCELLKDLA 68

Query: 63  LSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIE 122
           L GF NL VIDMD IE++NLNRQFLFR  D+G  KAE AA+ +  RV    + PHF +I+
Sbjct: 69  LMGFGNLHVIDMDTIELSNLNRQFLFRRTDIGASKAECAARFINARVPTCRVTPHFKKIQ 128

Query: 123 DKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKG 182
           D +  FY  FH++  GLDSI AR +IN +  S L Y+ D      +I PM+DGGTEGFKG
Sbjct: 129 DFDESFYQQFHLVVCGLDSIVARRWINGMLLSMLRYEEDGTIDTSSIVPMIDGGTEGFKG 188

Query: 183 HARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHS-GKAF 242
           +ARVILPG T C ECT+ LFPPQV +PLCT+A TPR   HCIEY  +I+W++ +  G   
Sbjct: 189 NARVILPGFTACIECTLDLFPPQVNYPLCTIANTPRLPEHCIEYVKIIQWEKQNPFGVPL 248

Query: 243 DPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLK 302
           D DD +H+ W+Y  A++R+  F I GVTY L QGVVK+IIPA+ASTNA I+AACALE  K
Sbjct: 249 DGDDPQHIGWIYERALERSNEFNITGVTYRLVQGVVKHIIPAVASTNAAIAAACALEVFK 308

Query: 303 IVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCG--PGVL-IELDSSITLQKFIDL 362
           + + C  +++NYL +N  +G++T   E  K ++CL C   P  L IE  ++ TL+  I L
Sbjct: 309 LATSCYDSMANYLNFNDLDGIYTYTYEAEKSENCLACSNTPQPLPIEDPNTTTLEDVIKL 368

Query: 363 LEDHPKLLLSKASVT-----HRGKNLYMQAPPVLEEMTRSNLGVPLFDL 403
           L D P+  L   ++T      + + LYM     +EE TR NL   L +L
Sbjct: 369 LCDSPRFQLKSPALTTVMKDGKRRTLYMSGVKSIEEATRKNLTQSLGEL 417

BLAST of CmUC05G086250 vs. ExPASy Swiss-Prot
Match: Q99MI7 (NEDD8-activating enzyme E1 catalytic subunit OS=Rattus norvegicus OX=10116 GN=Uba3 PE=1 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 1.1e-102
Identity = 198/402 (49.25%), Postives = 262/402 (65.17%), Query Frame = 0

Query: 10  RSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNL 69
           R   + K L R G     +FEP ++    L    KVLVIGAGGLGCELLK+LALSGFR +
Sbjct: 37  RWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQI 96

Query: 70  EVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFY 129
            VIDMD I+V+NLNRQFLFR +DVG+PKAEVAA+ + +RV   N++PHF +I+D    FY
Sbjct: 97  HVIDMDTIDVSNLNRQFLFRPKDVGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFY 156

Query: 130 NDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLE-ETIKPMVDGGTEGFKGHARVIL 189
             FHII  GLDSI AR +IN +  S L Y  +D  L+  +I P++DGGTEGFKG+ARVIL
Sbjct: 157 RQFHIIVCGLDSIIARRWINGMLISLLNY--EDGVLDPSSIVPLIDGGTEGFKGNARVIL 216

Query: 190 PGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVH---SGKAFDPDD 249
           PG+T C ECT+ L+PPQV FP+CT+A  PR   HCIEY  +++W +      G   D DD
Sbjct: 217 PGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGDGVPLDGDD 276

Query: 250 SEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSG 309
            EH++W++ ++V+RA  + I GVTY LTQGVVK IIPA+ASTNA+I+A CA E  KI + 
Sbjct: 277 PEHIQWIFQKSVERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATS 336

Query: 310 CSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGP-GVLIELDSSITLQKFIDLLEDHPK 369
               L+NYL +N  +GL+T   E  + ++C  C      I+   S  LQ+ +D L +   
Sbjct: 337 AYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSAS 396

Query: 370 LLLSKASVTH--RGKN--LYMQAPPVLEEMTRSNLGVPLFDL 403
           L +   ++T    GKN  LY+Q+   +EE TR NL   L +L
Sbjct: 397 LQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKEL 436

BLAST of CmUC05G086250 vs. ExPASy Swiss-Prot
Match: Q8C878 (NEDD8-activating enzyme E1 catalytic subunit OS=Mus musculus OX=10090 GN=Uba3 PE=1 SV=2)

HSP 1 Score: 374.8 bits (961), Expect = 1.4e-102
Identity = 197/402 (49.00%), Postives = 262/402 (65.17%), Query Frame = 0

Query: 10  RSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNL 69
           R   + K L R G     +FEP ++    L    KVLVIGAGGLGCELLK+LALSGFR +
Sbjct: 37  RWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQI 96

Query: 70  EVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFY 129
            VIDMD I+V+NLNRQFLFR +DVG+PKAEVAA+ + +RV   N++PHF +I+D    FY
Sbjct: 97  HVIDMDTIDVSNLNRQFLFRPKDVGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFY 156

Query: 130 NDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLE-ETIKPMVDGGTEGFKGHARVIL 189
             FHII  GLDSI AR +IN +  S L Y  +D  L+  +I P++DGGTEGFKG+ARVIL
Sbjct: 157 RQFHIIVCGLDSIIARRWINGMLISLLNY--EDGVLDPSSIVPLIDGGTEGFKGNARVIL 216

Query: 190 PGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVH---SGKAFDPDD 249
           PG+T C ECT+ L+PPQV FP+CT+A  PR   HCIEY  +++W +      G   D DD
Sbjct: 217 PGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGDGVPLDGDD 276

Query: 250 SEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSG 309
            EH++W++ ++++RA  + I GVTY LTQGVVK IIPA+ASTNA+I+A CA E  KI + 
Sbjct: 277 PEHIQWIFQKSIERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATS 336

Query: 310 CSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGP-GVLIELDSSITLQKFIDLLEDHPK 369
               L+NYL +N  +GL+T   E  + ++C  C      I+   S  LQ+ +D L +   
Sbjct: 337 AYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSAS 396

Query: 370 LLLSKASVTH--RGKN--LYMQAPPVLEEMTRSNLGVPLFDL 403
           L +   ++T    GKN  LY+Q+   +EE TR NL   L +L
Sbjct: 397 LQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKEL 436

BLAST of CmUC05G086250 vs. ExPASy TrEMBL
Match: A0A0A0L8U2 (NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis sativus OX=3659 GN=Csa_3G149920 PE=3 SV=1)

HSP 1 Score: 898.7 bits (2321), Expect = 1.0e-257
Identity = 444/446 (99.55%), Postives = 446/446 (100.00%), Query Frame = 0

Query: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK+
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKS 240

Query: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDDSEHMKWVYSEA+KRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420
           DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN
Sbjct: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTA 447
           DKKTSCLRKLRVAFKGVDGVTDMDTA
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTA 446

BLAST of CmUC05G086250 vs. ExPASy TrEMBL
Match: A0A1S3AXQ8 (NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis melo OX=3656 GN=LOC103483734 PE=3 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 2.3e-257
Identity = 444/446 (99.55%), Postives = 446/446 (100.00%), Query Frame = 0

Query: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDDSEHMKWVYSEA+KRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420
           D+PKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN
Sbjct: 361 DYPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTA 447
           DKKTSCLRKLRVAFKGVDGVTDMDTA
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTA 446

BLAST of CmUC05G086250 vs. ExPASy TrEMBL
Match: A0A5D3D1R8 (NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00180 PE=3 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 6.7e-257
Identity = 443/446 (99.33%), Postives = 445/446 (99.78%), Query Frame = 0

Query: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240

Query: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDDSEHMKWVYSEA+KRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420
           D+PKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN
Sbjct: 361 DYPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTA 447
           DKKTSCLRKLRV FKGVDGVTDMDTA
Sbjct: 421 DKKTSCLRKLRVTFKGVDGVTDMDTA 446

BLAST of CmUC05G086250 vs. ExPASy TrEMBL
Match: A0A6J1C926 (NEDD8-activating enzyme E1 catalytic subunit OS=Momordica charantia OX=3673 GN=LOC111009338 PE=3 SV=1)

HSP 1 Score: 886.7 bits (2290), Expect = 4.0e-254
Identity = 435/449 (96.88%), Postives = 444/449 (98.89%), Query Frame = 0

Query: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLV+GAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVVGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRL+DVGKPKAEVAAKRVMERVSGVNI+PHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLQDVGKPKAEVAAKRVMERVSGVNIVPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYIN+VACSFLEYDSDD+PLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINAVACSFLEYDSDDSPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK 
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKT 240

Query: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDD  HMKWVY+EAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDPVHMKWVYNEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFV+DKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVRDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420
           DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNL VPLFDLMGKVLKDVVHVTGVASKN
Sbjct: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTAIQV 450
           DKKTSCLRKLRVAFKGVDGVTDMDTA+ +
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTAVDI 449

BLAST of CmUC05G086250 vs. ExPASy TrEMBL
Match: A0A6J1C8P8 (NEDD8-activating enzyme E1 catalytic subunit OS=Momordica charantia OX=3673 GN=LOC111009338 PE=3 SV=1)

HSP 1 Score: 885.6 bits (2287), Expect = 9.0e-254
Identity = 435/447 (97.32%), Postives = 443/447 (99.11%), Query Frame = 0

Query: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKD 60
           MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLV+GAGGLGCELLKD
Sbjct: 1   MAGSVVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVVGAGGLGCELLKD 60

Query: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCR 120
           LALSGFRNLEVIDMDRIEVTNLNRQFLFRL+DVGKPKAEVAAKRVMERVSGVNI+PHFCR
Sbjct: 61  LALSGFRNLEVIDMDRIEVTNLNRQFLFRLQDVGKPKAEVAAKRVMERVSGVNIVPHFCR 120

Query: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGF 180
           IEDKEIEFYNDFHIIALGLDSIEARSYIN+VACSFLEYDSDD+PLEETIKPMVDGGTEGF
Sbjct: 121 IEDKEIEFYNDFHIIALGLDSIEARSYINAVACSFLEYDSDDSPLEETIKPMVDGGTEGF 180

Query: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKA 240
           KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK 
Sbjct: 181 KGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKT 240

Query: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FDPDD  HMKWVY+EAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL
Sbjct: 241 FDPDDPVHMKWVYNEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300

Query: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLE 360
           KIVSGCSKTLSNYLTYNGAEGLHTKVTEFV+DKDCLVCGPGVLIELDSSITLQKFIDLLE
Sbjct: 301 KIVSGCSKTLSNYLTYNGAEGLHTKVTEFVRDKDCLVCGPGVLIELDSSITLQKFIDLLE 360

Query: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKN 420
           DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNL VPLFDLMGKVLKDVVHVTGVASKN
Sbjct: 361 DHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKN 420

Query: 421 DKKTSCLRKLRVAFKGVDGVTDMDTAI 448
           DKKTSCLRKLRVAFKGVDGVTDMDTA+
Sbjct: 421 DKKTSCLRKLRVAFKGVDGVTDMDTAV 447

BLAST of CmUC05G086250 vs. TAIR 10
Match: AT5G19180.1 (E1 C-terminal related 1 )

HSP 1 Score: 728.8 bits (1880), Expect = 2.7e-210
Identity = 355/443 (80.14%), Postives = 388/443 (87.58%), Query Frame = 0

Query: 6   VQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSG 65
           V  S++RDLDKLLLR GNL+   F PG  LRDD++ YV++LVIGAGGLGCELLKDLALSG
Sbjct: 10  VPQSKTRDLDKLLLRHGNLVDPGFVPGPGLRDDIRDYVRILVIGAGGLGCELLKDLALSG 69

Query: 66  FRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKE 125
           FRNLEVIDMDRIEVTNLNRQFLFR+EDVGKPKAEVAAKRVMERVSGV I+PHF RIEDKE
Sbjct: 70  FRNLEVIDMDRIEVTNLNRQFLFRIEDVGKPKAEVAAKRVMERVSGVEIVPHFSRIEDKE 129

Query: 126 IEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHAR 185
           IEFYNDF+IIALGLDSIEAR YIN VAC FLEY+ DD P  ETIKPMVDGGTEGFKGHAR
Sbjct: 130 IEFYNDFNIIALGLDSIEARKYINGVACGFLEYNEDDTPKRETIKPMVDGGTEGFKGHAR 189

Query: 186 VILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDD 245
           VILPGVTPCFECTI+LFPPQVKFPLCTLAETPR AAHCIEYAHLI+W+ VH GK FDPD+
Sbjct: 190 VILPGVTPCFECTIYLFPPQVKFPLCTLAETPRNAAHCIEYAHLIQWETVHRGKTFDPDE 249

Query: 246 SEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSG 305
            EHMKWVY EA++RAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVS 
Sbjct: 250 PEHMKWVYDEAIRRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSA 309

Query: 306 CSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDHPKL 365
           CSKTL NYLTYNG EGL+T+VT+F +D +CLVCGPG+LIELD+S+TL KFI++LEDHPKL
Sbjct: 310 CSKTLVNYLTYNGGEGLYTEVTKFERDTECLVCGPGILIELDTSVTLSKFIEMLEDHPKL 369

Query: 366 LLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGV-ASKNDKKT 425
           LLSKASV      LYMQAPPVLEE  R  L  PL+DLMG+V KD +HV G  A KN++K 
Sbjct: 370 LLSKASVKQGENTLYMQAPPVLEEFHRPKLSKPLYDLMGRVQKDTIHVFGQRALKNNEKE 429

Query: 426 SCLRKLRVAFKGVDGVTDMDTAI 448
           SC  K+RV FKG DGV DMDTAI
Sbjct: 430 SCTTKVRVVFKGADGVADMDTAI 452

BLAST of CmUC05G086250 vs. TAIR 10
Match: AT2G21470.1 (SUMO-activating enzyme 2 )

HSP 1 Score: 151.8 bits (382), Expect = 1.4e-36
Identity = 79/203 (38.92%), Postives = 118/203 (58.13%), Query Frame = 0

Query: 44  KVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAK 103
           KVL++GAGG+GCELLK LALSGF ++ +IDMD IEV+NLNRQFLFR   VG+ KA+VA  
Sbjct: 14  KVLMVGAGGIGCELLKTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHVGQSKAKVARD 73

Query: 104 RVMERVSGVNILPHFCRIEDKE--IEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSD 163
            V+     +NI  +   +++ E  ++F+  F ++  GLD+++AR ++N +  +       
Sbjct: 74  AVLRFRPNINIRSYHANVKNPEFDVDFFKQFDVVLNGLDNLDARRHVNRLCLA------- 133

Query: 164 DNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAA 223
                    P+V+ GT GF G   V + G T C+EC     P    +P+CT+  TP    
Sbjct: 134 ------ADVPLVESGTTGFLGQVTVHIKGKTECYECQ--TKPAPKTYPVCTITSTPTKFV 193

Query: 224 HCIEYAHLIKWDEVHSGKAFDPD 245
           HCI +A  + + ++   K  D D
Sbjct: 194 HCIVWAKDLLFAKLFGDKNQDND 201


HSP 2 Score: 51.6 bits (122), Expect = 1.9e-06
Identity = 42/144 (29.17%), Postives = 70/144 (48.61%), Query Frame = 0

Query: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FD DD   +++V + A  RAE FGIP  +    +G+  NI+ A+A+TNAII+    +E +
Sbjct: 336 FDKDDQLAVEFVTAAANIRAESFGIPLHSLFEAKGIAGNIVHAVATTNAIIAGLIVIEAI 395

Query: 301 KIV-SGCSKTLSNY-LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDL 360
           K++     K    Y L +   + L   +  +  +  C VC    L+ L+ +    K  DL
Sbjct: 396 KVLKKDVDKFRMTYCLEHPSKKLLLMPIEPYEPNPACYVCSETPLV-LEINTRKSKLRDL 455

Query: 361 LED--HPKLLLSKASVTHRGKNLY 381
           ++     KL ++   + H    LY
Sbjct: 456 VDKIVKTKLGMNLPLIMHGNSLLY 478

BLAST of CmUC05G086250 vs. TAIR 10
Match: AT2G21470.2 (SUMO-activating enzyme 2 )

HSP 1 Score: 151.8 bits (382), Expect = 1.4e-36
Identity = 79/203 (38.92%), Postives = 118/203 (58.13%), Query Frame = 0

Query: 44  KVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAK 103
           KVL++GAGG+GCELLK LALSGF ++ +IDMD IEV+NLNRQFLFR   VG+ KA+VA  
Sbjct: 14  KVLMVGAGGIGCELLKTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHVGQSKAKVARD 73

Query: 104 RVMERVSGVNILPHFCRIEDKE--IEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSD 163
            V+     +NI  +   +++ E  ++F+  F ++  GLD+++AR ++N +  +       
Sbjct: 74  AVLRFRPNINIRSYHANVKNPEFDVDFFKQFDVVLNGLDNLDARRHVNRLCLA------- 133

Query: 164 DNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAA 223
                    P+V+ GT GF G   V + G T C+EC     P    +P+CT+  TP    
Sbjct: 134 ------ADVPLVESGTTGFLGQVTVHIKGKTECYECQ--TKPAPKTYPVCTITSTPTKFV 193

Query: 224 HCIEYAHLIKWDEVHSGKAFDPD 245
           HCI +A  + + ++   K  D D
Sbjct: 194 HCIVWAKDLLFAKLFGDKNQDND 201


HSP 2 Score: 51.6 bits (122), Expect = 1.9e-06
Identity = 42/144 (29.17%), Postives = 70/144 (48.61%), Query Frame = 0

Query: 241 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETL 300
           FD DD   +++V + A  RAE FGIP  +    +G+  NI+ A+A+TNAII+    +E +
Sbjct: 336 FDKDDQLAVEFVTAAANIRAESFGIPLHSLFEAKGIAGNIVHAVATTNAIIAGLIVIEAI 395

Query: 301 KIV-SGCSKTLSNY-LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDL 360
           K++     K    Y L +   + L   +  +  +  C VC    L+ L+ +    K  DL
Sbjct: 396 KVLKKDVDKFRMTYCLEHPSKKLLLMPIEPYEPNPACYVCSETPLV-LEINTRKSKLRDL 455

Query: 361 LED--HPKLLLSKASVTHRGKNLY 381
           ++     KL ++   + H    LY
Sbjct: 456 VDKIVKTKLGMNLPLIMHGNSLLY 478

BLAST of CmUC05G086250 vs. TAIR 10
Match: AT2G21470.3 (SUMO-activating enzyme 2 )

HSP 1 Score: 151.8 bits (382), Expect = 1.4e-36
Identity = 79/203 (38.92%), Postives = 118/203 (58.13%), Query Frame = 0

Query: 44  KVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRLEDVGKPKAEVAAK 103
           KVL++GAGG+GCELLK LALSGF ++ +IDMD IEV+NLNRQFLFR   VG+ KA+VA  
Sbjct: 14  KVLMVGAGGIGCELLKTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHVGQSKAKVARD 73

Query: 104 RVMERVSGVNILPHFCRIEDKE--IEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSD 163
            V+     +NI  +   +++ E  ++F+  F ++  GLD+++AR ++N +  +       
Sbjct: 74  AVLRFRPNINIRSYHANVKNPEFDVDFFKQFDVVLNGLDNLDARRHVNRLCLA------- 133

Query: 164 DNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAA 223
                    P+V+ GT GF G   V + G T C+EC     P    +P+CT+  TP    
Sbjct: 134 ------ADVPLVESGTTGFLGQVTVHIKGKTECYECQ--TKPAPKTYPVCTITSTPTKFV 193

Query: 224 HCIEYAHLIKWDEVHSGKAFDPD 245
           HCI +A  + + ++   K  D D
Sbjct: 194 HCIVWAKDLLFAKLFGDKNQDND 201


HSP 2 Score: 54.7 bits (130), Expect = 2.3e-07
Identity = 46/162 (28.40%), Postives = 75/162 (46.30%), Query Frame = 0

Query: 223 CIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIP 282
           C+E  HL           FD DD   +++V + A  RAE FGIP  +    +G+  NI+ 
Sbjct: 331 CVEIGHL----------TFDKDDQLAVEFVTAAANIRAESFGIPLHSLFEAKGIAGNIVH 390

Query: 283 AIASTNAIISAACALETLKIV-SGCSKTLSNY-LTYNGAEGLHTKVTEFVKDKDCLVCGP 342
           A+A+TNAII+    +E +K++     K    Y L +   + L   +  +  +  C VC  
Sbjct: 391 AVATTNAIIAGLIVIEAIKVLKKDVDKFRMTYCLEHPSKKLLLMPIEPYEPNPACYVCSE 450

Query: 343 GVLIELDSSITLQKFIDLLED--HPKLLLSKASVTHRGKNLY 381
             L+ L+ +    K  DL++     KL ++   + H    LY
Sbjct: 451 TPLV-LEINTRKSKLRDLVDKIVKTKLGMNLPLIMHGNSLLY 481

BLAST of CmUC05G086250 vs. TAIR 10
Match: AT5G06460.1 (ubiquitin activating enzyme 2 )

HSP 1 Score: 101.3 bits (251), Expect = 2.1e-21
Identity = 68/206 (33.01%), Postives = 110/206 (53.40%), Query Frame = 0

Query: 32  GSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFR-----NLEVIDMDRIEVTNLNRQF 91
           GS L+  L+   +V V+GAG LGCE LK+LAL G        L V D D IE +NL+RQF
Sbjct: 480 GSTLQKKLED-ARVFVVGAGALGCEFLKNLALMGVSCGTQGKLTVTDDDVIEKSNLSRQF 539

Query: 92  LFRLEDVGKPKAEVAAKRVMERVSGVNI--LPHFCRIEDKEI---EFYNDFHIIALGLDS 151
           LFR  ++G+ K+ VAA       S +NI  L +    E + +    F+ +  ++   LD+
Sbjct: 540 LFRDWNIGQAKSTVAATAAAGINSRLNIDALQNRVGPETENVFDDSFWENLTVVVNALDN 599

Query: 152 IEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWL 211
           + AR Y++S  C + +            KP+++ GT G K + ++++P +T  +  +   
Sbjct: 600 VTARLYVDS-RCVYFQ------------KPLLESGTLGAKCNTQMVIPHLTENYGAS--R 659

Query: 212 FPPQVKFPLCTLAETPRTAAHCIEYA 228
            PP+ + P+CT+   P    HC+ +A
Sbjct: 660 DPPEKQAPMCTVHSFPHNIDHCLTWA 669

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004134149.12.1e-25799.55NEDD8-activating enzyme E1 catalytic subunit [Cucumis sativus] >KGN57012.1 hypot... [more]
XP_038897781.13.6e-25799.33NEDD8-activating enzyme E1 catalytic subunit [Benincasa hispida][more]
XP_008438709.14.7e-25799.55PREDICTED: NEDD8-activating enzyme E1 catalytic subunit [Cucumis melo][more]
KAA0049385.11.4e-25699.33NEDD8-activating enzyme E1 catalytic subunit [Cucumis melo var. makuwa] >TYK1717... [more]
XP_022138079.18.4e-25496.88NEDD8-activating enzyme E1 catalytic subunit isoform X1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
O650413.8e-20980.14NEDD8-activating enzyme E1 catalytic subunit OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q54QG93.9e-10548.79NEDD8-activating enzyme E1 catalytic subunit OS=Dictyostelium discoideum OX=4468... [more]
Q9V6U85.1e-10548.90Nedd8-activating enzyme E1 catalytic subunit OS=Drosophila melanogaster OX=7227 ... [more]
Q99MI71.1e-10249.25NEDD8-activating enzyme E1 catalytic subunit OS=Rattus norvegicus OX=10116 GN=Ub... [more]
Q8C8781.4e-10249.00NEDD8-activating enzyme E1 catalytic subunit OS=Mus musculus OX=10090 GN=Uba3 PE... [more]
Match NameE-valueIdentityDescription
A0A0A0L8U21.0e-25799.55NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis sativus OX=3659 GN=Csa_3... [more]
A0A1S3AXQ82.3e-25799.55NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A5D3D1R86.7e-25799.33NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis melo var. makuwa OX=1194... [more]
A0A6J1C9264.0e-25496.88NEDD8-activating enzyme E1 catalytic subunit OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1C8P89.0e-25497.32NEDD8-activating enzyme E1 catalytic subunit OS=Momordica charantia OX=3673 GN=L... [more]
Match NameE-valueIdentityDescription
AT5G19180.12.7e-21080.14E1 C-terminal related 1 [more]
AT2G21470.11.4e-3638.92SUMO-activating enzyme 2 [more]
AT2G21470.21.4e-3638.92SUMO-activating enzyme 2 [more]
AT2G21470.31.4e-3638.92SUMO-activating enzyme 2 [more]
AT5G06460.12.1e-2133.01ubiquitin activating enzyme 2 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014929E2 bindingSMARTSM01181E2_bind_2coord: 344..435
e-value: 1.3E-37
score: 140.9
IPR014929E2 bindingPFAMPF08825E2_bindcoord: 345..434
e-value: 1.0E-22
score: 80.0
NoneNo IPR availableGENE3D3.40.50.720coord: 41..342
e-value: 1.2E-65
score: 223.4
NoneNo IPR availableGENE3D3.10.290.20coord: 343..430
e-value: 4.2E-16
score: 60.6
NoneNo IPR availablePANTHERPTHR10953UBIQUITIN-ACTIVATING ENZYME E1coord: 9..414
NoneNo IPR availableCDDcd01488Uba3_RUBcoord: 44..339
e-value: 0.0
score: 563.518
IPR000594THIF-type NAD/FAD binding foldPFAMPF00899ThiFcoord: 36..335
e-value: 6.0E-71
score: 238.6
IPR023318Ubiquitin activating enzyme, alpha domain superfamilyGENE3D1.10.10.520Ubiquitin activating enzymes (Uba3). Chain: B, domain 2coord: 211..279
e-value: 1.5E-31
score: 110.2
IPR030468NEDD8-activating enzyme E1 catalytic subunitPANTHERPTHR10953:SF6NEDD8-ACTIVATING ENZYME E1 CATALYTIC SUBUNITcoord: 9..414
IPR033127Ubiquitin-activating enzyme E1, Cys active sitePROSITEPS00865UBIQUITIN_ACTIVAT_2coord: 209..217
IPR035985Ubiquitin-activating enzymeSUPERFAMILY69572Activating enzymes of the ubiquitin-like proteinscoord: 44..406

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmUC05G086250.1CmUC05G086250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045116 protein neddylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0019781 NEDD8 activating enzyme activity
molecular_function GO:0008641 ubiquitin-like modifier activating enzyme activity