CmUC05G083350 (gene) Watermelon (USVL531) v1

Overview
NameCmUC05G083350
Typegene
OrganismCitrullus mucosospermus (Watermelon (USVL531) v1)
DescriptionMalic enzyme
LocationCmU531Chr05: 2613097 .. 2625250 (-)
RNA-Seq ExpressionCmUC05G083350
SyntenyCmUC05G083350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGAAGTGGAATTCTCTTTCTGCTTCGCTTATGAAGCGTCTCAAGCTTCACTACCAGATGACCCACAATATTCCAGCGTTAGCTCAGGCGAGATCCTTCACCACAAGCGAGGGGCATCGTCCCACCATTGTTCATAAACGTAGCCTCGATATTCTTCACGATCCGTGGTTTAACAAAGTAAGCCTTTCGATACTCTTTCCATTTCGCTCTCTTTTCTTATCTGATTTGCTTCTGTTGTTCTGTATTTTCGTTTTAATACACTCTCTGTAGCCTGTGAGATGCGGTACTTGGTGCCCGTGCGTACTCCAGTGATTAGCGAGGATGATAATCTGTATTAGAAATTAAGATTAGAGGAATTATCAATTACGTGTTGTAATTAAGTGGCGGAAACACTTACTCTTAGAAATTGTGTAACAAAATAATTAACTACTACTCAAATTCATTGATGAATCTCTAGGACTAAATCACTACATGATTATTGATAATGGTTCTGGTTCTTGAGCATCCGAATGTTACCGTCGGATACTGGATCGAGCTGGGGTAACTTTGTTAAGAATTAATTATACAGAGTAGAGCTTTCTTTTGTAAGTGGTACTGGCTACTATACAAACTGGTGGTGATCCAAGGTTATTAGATCGGTGTTTATAGAATCCTAGCTAATTATATGGTGTGTGAAAGTATGAAGCGGTTATCAAGTTTTGCCCAATTATTGGGTGAAATTAAAGGGTACTGTATGGTCGGCACTGATTTTATTTATTTATATCTGTTAATGCTCTGCATTTTCTCCCCCTTTAATTGGCACTACTTTTTAGCTTTTATGAAGAAAAAAACAGAGATGCCACGAGCGAAATTCTTTCGTACCATTTTTTTAATGTTAAATTGTGCACTTACATTTGAAAATAATCTCATAAGAAACTATCCCTCTGTTCTTTTCAAAATAACTTTATCGTATGGAAAGAATCCAATTCATTATTTTGTAGGTATTGATATTCACTTCATGATTATGAAATGAAAAAAATGCTATCGCCAAATCCCAGATCAATTATTCTCTAAATGCAACTTCTATTACTATTACGTTTCATGACTTCTTCATTTCATTGAAAGAAGGGAAATGGTTTACTTTGAAGAATACCTTTTTCCTTTCCAAATGGTCCACTTATATTCTTCGAACCTAAAATGAAGTTCAAAACTATCACGGGCATTGGAAAGTGAATGTAATGTAATTTGGAGCTAATTCTATCAGTGTTTATATTATTATGTTGCTTTGGTATAAGGGTCATTTATAGACATCCTTTCTTCGTTATCATCATAGTAGGGAACTGCCTTTACGATGACAGAACGTGACCGGCTTGATCTTCGAGGACTTCTTCCACCAAATGTAATGTCTTCAGAGCAGCAAATTGAACGCTTTAGTAAGTTCTTAAGACTTTTATTTTCCATGGAGCCATTGAGAATCAAATACCATTTATTTTATATTTCTTAGCATTTCCTTTCTTGGAATTTTAGACACAAATTTGTACATCGGTGCCTGTTAGTTGATATGTGGTTTCAACATTGTGTACATGTCTTAAGAGAGTGTGCGAGCATGACAACAATGAATACTGCGCTCTTTCTTTTAAGAAGTTTCTCCAGTGTTGTCTACTTTTATTTCTGTTTAATTTTATGCTTTAGTTGTTAAATAGTTCATACATTTGAAATGATTGTTAGCAAAATCTCTCTTTATTATTGAACAAGATTATCCTTGTCATCACTGGTGCACGTTCAATATAGCAGCTCTGAATTCCTTTCAATTATATTGGAGCAACAAAAAAAAAAAAAAAAAAAAATATTGGAGCAACATTCCTTCATTTGTTTATTATAGTTCTTCTTGAGTTTATAGGTTGTATTCAATTTACATTCATTAATTTCCTTGCTGCTTAAACTCCAAATTTGTCTTCTAGATAATATGCAACTTCAACTTTTCATCTTGTTCTTTAATTTGATCTTGTGACCGACAACAGGTTTCTTGTAGTTCTGGGCGCGACTTCTTCTGTTTAAATTTAGGAACCAAAATCTATGTAGCTTTAATCTACATGTCATAACTCTAGATAATTTACAGTGGTTGACCTGAAGAGGCTTGAAGTGCAAGCAAGAGATGGGCCTTCAGATCCTAATGCTTTGGCCAAATGGCGAATTCTGAATAGATTGCACGATAGAAACGAGACAATGTACTATAAAGTGAGTTAAGGTTGCTCATCTACCAGTGTAGACAATTTCAAGGAATTTTCCCAGACTTGTTCTCATGAATTCTCGATCTAATCTAGCTAGTCTTTGCTTTATAGATCTATACAATCTTATTATATTAATCTTGTTTTTGCTTATGAATTATTGTTAACACTGATCAACTTATGCTATGTAGTTAACCATCATGGGTTGGCCTAGTGGTAAAAAGAGAGACATAATCTCAATAAATGGCTAAGAGATCATGGGTTCAATCTATGGTGGCCACCTACCTAGGATTTAATATCTTATGAGTTTCCTTGACATCCAAATGTTGTAGGGTCAGGTGGGTTGTCCCGTGAGATTAGTCGAGGGGAGCGTAAGTTGGCCCGGACACTCACGGACATTAAAAAAAAAAAGTTAGGGAAAGGATATTTCTTCTTATAGAATAAAGTAAATTTTCTTTTGGTTAATTTGTTTTCTAATGTTTGATTTAGTAAGAGATCTCCTGGAAGGGTTTTTTATTTTTTATTTTATTTTATTATTTATTTTTTAAGGAAGAATTAAACATGCCACAAAAATTTTAACACATTAATGACATTGTCTACTGAATATGTTGAATGATATCTTCCATTCTTCTACTGTCGAAAAAATGCAGCTTCACAATTTATTAGAAGTGCTTCAATTTTAAAGTTGTGAAAGATTTTAAAGTGGTCATCCATGATGTTAAACCACTAATCAACCCAAAAGAAAATTTAATCACATCTGACACTTCAACTCATCACATTCAAGTTATTCTAAAAAATATAAAAATAACAAATTTTCAGATGGTTTCATTTCTCAGCGTGCGTATATTCTTACTTATACTGTAATAAAGTACTTTTGCACTGTCATATTTTTTTGAAGAAATGCAGTTATTTGTCTTATGATTTATTTTCCATATCACAAGTCATCTAATTAAATGTGACTTTGACAGGTTTTGATTGCTCACATTGAGGAGTATGCACCTATAGTATATACACCAACTGTTGGTCTTGTTTGCCAGAACTATAGTGGCTTATTTAGAAGACCAAGGGGAATGTATTTTAGTGCAGAAGATCGAGGAGAAATGATGTCCATGGTTTATAATTGGCCTGCTGATCAGGTTTTCTTCAAGTTTCTTTGATGCTCATAACAGTCAATTCATTGAACCAATTCAAATCTTTCAGAAGCCAAATAGTTTCTACTTCTACTTTTAAGAATTGGAACTCTATATATAAAGTATATTGAAAACTGAATAACTTTTTTGCAGGTTGATATGATTGTTGTTACTGATGGAAGTAGAATATTAGGTCTTGGAGATCTCGGAGTTCACGGGATTGGGATTGCCATCGGAAAGTTGGATTTATATGTTGCTGCTGCTGGGATAAATCCTCAGAGGGTAGGTTCTTGCTTCTAGATCTCTTATGTGTTGAGTTCCCTAGCATGATTACCAAGTAGCTGGTAATGTTTTGGAATTTATTAATTGGTTCAGAAACTTTTATTTGTGTTGAAAAAATTATTTAAACAGAGCCATTCACGGTAGCATGAAGAATTTGTCATATGAGCAGCTAAGCGTTTTTTTCCCCACAAGCCACTTCAAACTAACAGGCTTTCTTGAGATTATTTTCAGTTTGGGTTATTATTAATTCCTTTCCAGTTTCAGTTTGGGTTATTAAACTTCAATTTTTGAGTTGTCTTTAGAAGGATGTTCTTTCAGTGTTTTCTTTCTTTATAACTTAACAAGCAATGAAAATATCTTCTTGTGCAAGTTACAATAGGTGCTTCCAGTCATGATTGATGTTGGAACCAACAATGAAAAGCTTCTGAAGGATCCCTTATGTAAGTACTTCCATACTATTAGGTGGATAATGTGCTAAATTCTCTCTCTCACATAAAAGAGTTGTCTTAATGAAGGCTTGATTTTTAATAATAATAATAATAATAATAATAATAATAATAATAATTAACTAAATCTACAATCTGATGACCAATCATTTATAGTATGTATAAAATCCCTTTTTGACTTGGTCAGGGGCCGTACCTTTCCTTGCCTCATGTTTGAATTGAGATTAGGAACATGCACAAAATGCTGTAGGGAAACTGTTGCATGGCTTCATGCATGTTCCACGTCTTAATCATCATACAAAATTCACTTATACTTAATACTGTAATGAGATGATTTCCAAAGCTTTAAGAAAAAAGGTACGTTATTGATTGAATGAGCACAACTATTTCCATCTCATAATGTGTAAAATTTTTGTATTGTTTTCACGATGGATCAACATCTTCAAAACATCCATAAATTGCTCGATATTCTCTAGCTGTGTTTAGTTTTCAACTTTTCATCACTAGTTCACATCTTTAAAATAACCGATTACAATTTTAAAGCGACATTGGGATGATGCTTTCATGTTTTAGCTTTTAATTTTATTGGTATAGCATTTTGAATTAATCCTTTTTTGGTAGATTTAGGATTGCAAGAACACCGATTGGATGGTGATGAATATTTGGCCATTATCGATGAATTCATGGAGGCTGTGTTTACTCGCTGGCCACATGTTATTGTTCAAGTAATTCAACTGCTACTTTGGTCTAATAACTGGAGTTTCTATTCTTATTTTATATTACATTTTTAAACCATGCAATAAATACTTAACAACTGTTCAACAGTTTGAAGATTTCCAAAGCAAATGGGCATTTAAGTTATTGCAACGATACAGAAATAACTACAGAATGTTTAATGATGACGTTCAGGTTGACCATTCTCTCCTTTCTTCTTCTACTTGATTTCTTTCCTTTTTCCCGATAGTTTGCTTTGTTTACATTGATTTCTTGTACTATACTGATATGTCAACTTATCTAGATTGATTTGACATTTTCCTTTCTTAAACTCTTAGCTATTTATTTATATAGGAACCATACTTTTTTTTCCCTTCTCTAAAACTTAGCTATTTAGATTATTATGTTTTCCATGTATGCTTTTTTTATAAATTTTTTTTATGGTGGTTGCTAAACACACTTGCTGGTTTATACCATGATCATCCAGCAGTCAACTCTAACAATTCAGTGTCAAACTGTCTCTAGGGAACGGCAGGAGTTGCTATTGCTGGACTTTTAGGGGCTGTGAGAGCACAAGGAAGACCGATGATTGACTTTCCAAAGCAAAAGATTGTTGTTGCTGGTGCTGGAAGGTAGTTGCATTCCTTATTAGTTTTATCTAACATTATAAGATAGAAGGATGCAATTCAGAATTTTGTGGTGATAGTATTCATAGTGCCCCAAGCCTTGTTTATGTTAGCAAAGCATACAAAACTTCCAACCATATAATCTTTCTATTTTGTCCTAACTGAATTTCTGTCAATTGTTCCATTTATTAACAATTGACACTGTTACTTTCATATTTTTAGTACATGCGTAAAACTTAAGTTTTAGCTTATTATTATTTTTTTAATGATGCAGTGCAGGAATTGGGGTTTTAAACGCGGCAAGGAAAACCATGGCGAGGATGTTGGGAAACAATGAATCAGCATTCGAGGCTGCCCGAAGCCAATTTTGGGTGGTAGATGCCCAGGTGATCAACTGCTAGTCTTTCAGTTCTTAAGCTAAAAGTTTTTACATTTAAAAGCTAACAGTAAATTTATTACACGACTATTCTGTTATGTCTTTTATACTTAAAACCATGCCACTTAATGATTTTCTTGGGAGAATGAAAAATTAAAAAGTTCCCTTTAAATCTGAAGTTTTTAATGGCCTGCTATACATTTTTCTCCAACATTTTAAACAAAAACTAAATATACATACGTTTTGGTTCTCAATGTTCGTATAAAACGAAGGGTCAAGCACTTTAAGAAAAAGCCTTTAGCCATGAGTTCGAAAACTTTGGACCTACCTATTTGGCACAATAGATATCTCATGAGTTTGTGGCCTACTAATAATGTAAGGTCAGGCAATACTTCCCAGTTTTGGAAGATGAGATTTTATGGTCTGCAAGTGTTTGGTAAAAATAAAAAGTATAAATTGTTTGTTACTTTGTGCACATTTCTTTGTTTTATGTTAACTCGCCCAATTAGGATAAATGGTAATTTTGCTCTCACAATGCTCGTAGAATTGAAGACATAGATTTATTAAATGTAAAAAACGAAGAAGCCTTTTCTTCTAGGAGACAAATTTTCATCAAGTAAAACATGAAGTTGAATCTCGAGTATGTAGCAGCTGATTGAAAATCTCTTTTCGCATTTCAAATATTTTCTTTATGTGCCCTAGGGCTTTTCCGCCCACTTCCTTGAGTTCATTATTTTAAAGTATATTTTATATTATTTTGTTTGTCAACATATTTCCAAGTCATTAGCTTACTTGTGTTACTGAGTTCAGCCAACATTTTCCCGTGCATGAATATTTCTTGCTTACATTTGCAATAACCTTCATTGTCGTAGGTTACGTCCATTATACTATGGATGCTGCTATCCTTCAAGGGGTACATGGGTGATGTGGGATCAAACTTTTCGTACATTTGCTAATTGTTTGTTTTTTTGGTTGTTGTCAGGGTTTGATCACAGAGGAAAGAGAAAATATTGATCAAGATGCAAGTCCGTTTGCAAGGAAGGTCAAAGAAATTAATCGTCAAGGATTGAGGGAAGGTGCAAGTCTAGTGGAAGTGGTTTGTAAATTTCCCATTCTTCTCACTCATAGGCTTATAGCTACTTTTGGATATAATGATAATTTTGACGTTGACATTTAGAAATTGAAAGTTGTTGGAAAAGGTGGAATTTGAGTGGAGAACCTAAATAATTGTTGCAGGAAAATACTTCAATCTTGTGAGGCATCATAATTTCATTGTTAGGATATAAATGTATTAGTCAATTTAATACTAATGAACTATTCTATGAGTGACCTCGTGAAGTGCTTGGTGGTAAGAGGTTAGGATGTTTGAAGTTACCCTCCAATGCTTTGGGTTATATTCTCTTGGTTGAAAATTAGGGTGCGTTTGGTTAGTGAATGTATACATGCATTTTTAACACATTGAACGCGAGTGCATGGAAATTATAGATCCTTCTGGTGTTAGTTTACTTCAGTGTGATTTTTCTATTTTTATTTAAATGGTGTATTTTAATTCTATCGTCCCCATATGAGACATATTAATTTTCTGCCATTTCCTTGTAAATGTTATATTAGGTGCAACAAGTGAAACCTGATGTGCTTCTGGGATTGTCTGCCGTTGGTGGATTGTTCACAAAGGAGGTATTAAGCATGGGCATGGCTTTTTTTATGCAAAATCATTTGTAGTCAATATTCACTTGTCTCCTTAATATTTTCTTTCCGGGGGATAACAAACTGACTGTTCTTTCAGTTATTTGTGCCTTATAATTGTGTGTTAGATTTCTATTGATTCAAACACTACACCTACTGCCTACAATGGATGGAGAACGAGGGGAAAAGAGAATTGGAAGATTTCCATTTCTGTATTCTATGGGAGCCCCTATGATGGGACACAAAAATGACGGCTGAAAATAGAAACAAAAGTCAGTGAAAAAAATATGCAAATAAAAATGATATAGACTAGGACAGATCTAAATGAATGACAATGTAACTGAATTTTTTAGCTTGTGCATAGTTTAGGATTTAAGAAATAAATGATACCAATCACCCGAATGTGAAACTTGTGTAATTTACTCTTCTTATACAACATGTGTGTAGGTGTTGGAGGCTCTTAAAGGATCAACAGCTACAAGACCAGCTATTTTTGCCATGTCTAATCCTACAACAAATGGTACAGTTGTAGAATCTATGAATTTCTCTACCTGTGTATAGGTCACAAATCTCGCCCTTCAGCTGGTTGGTAATTCTTACAGTTTGTATATCTATCTCATGTAGCCGAGTGCACTCCTCAAGAAGCATTCTCAATCTTGGGTGAAAACATCATATTTGCAAGTGGAAGCCCATTCAAAGATGTCGATTTTGGTATGTGAAATGAATGTGTCATATGCTTTAATGCTTTTTACTTCTGTTTTAAGATCTTAAAACAAGGAAGAGGTATGTTTCTAAAAAAATTAAGCTCAAGAGCATTATCTCGGATCTTTTCTTTTAAAGGTGTTGGAAAAATAAGTAAAAGTTTACTCGCAGCCAGCAAGAATGCACAAAATTTTTGGATGCATTCAGTGTGAAATAATGCTCTTGAAACTAGTGAGATTGGGAGGCACTTTTAAACGTTTACTTAGCTTGAACGTCCTGTTTCAGATAACATTCATTTTTAAAATCCCTTTATTTTTTCAAGAGATAGAAGTAAGTTTTGCTGATTATTTGGACTTCTATTTAACGACATAGCTCTGAGTGGTTAATGGGAATGGGAGAGCGTTTATCTTTCAACTAAATTTGATTAGCGAGTACCTCCCTTCTTTCATTGTGCATCCATAATTTGAGAAATTATTTTGCGAAAAATGTTTCTTCTCGGTGTAATTTCTTTCTTAAAAAGGGAAAACAAATCAAGGATAGAAACTTTAGTGCGTTATATTATCTTCATTATAATAACAATGATAATTTTGTAGTAACTATAATTTGGTAAGTAGTCCTTTCATGACTTGCCTTTTTGTTCGTCGATCATGAATGTTGGAACTAGTTCTTTAGTTTCATACTAGAGACAAACCTTCAATCTATTCAATTTCTATGAGTTCAAATATATTAAATACAAATGTTTTTCTTTCTACAGTCTTTTGGAAAGTGCTCTAATATAATTGCAATATTCTCCAGGGAATGGTCACATTGGCCACTGCAACCAAGGAAACAACATGTATCTTTTCCCAGGGTTAGTTTGCCAAGTTGCACATTGTAATGGCAATAAAGAGAATATTTTATGAGATGTGAAGTTTTTAATATGTCTGGATGCATTTTTGTGTAGTATTGGGCTCGGTACGCTTCTATCTGGCTCTCCTATCGTATCTGATGGCATGCTGCAGGCTGCTGCTGAGTGGTAAGGCTTCTGTTCTTTCAAGCTTTTGTAGATCAAATTTTGTTGATGGCAAACCTCTGAGGAACAATTCCTAAAATTTTAGAAGGATGGAATCTAGTCATTTTTCAAAATAGTAGTGTAACCTTCTGCGGCTGCAGGTGCAGTAGCGATAAAGAACTCATTGGCTGGAAAAGGAATTTTGTGTTTTCTGGATTAAAAGAAAATTTTCACTGATAAGTGGGATCCACACGGAAATGCTCCAAAACCTAGTCAAAGAGAGGCATGAAAAATAACATTTTCTTGAATTGGCATAAAAATGAGGGAGAATAATTATAATTCTTTTTGGAGAAATTATTTTAAATCACAAAACTGCTGAAAATATTTATAAATAATAGCAAAATATCACAGTTTATTTGCGATAGTAGTAAATTGCAAATTTTGTTATATTTGTGAATATTTTGGTTCATTTTCTTATATTTGAAAACAGCCATTCTTTTAGAATACAAGAGACCTTATTTATTTCTTAAATAATTTCTCAGTTTTTTTAAAACCTTTCTTGCACATTAGATCTAATAGCATTTGAGTCGCAGAATTTTCAGCCTTGGACAAAAGACGAATGACCACAAAACATATTCAGTAGGAATAATTTCTTCGTATAGGGAAAGATACTTTTACTTCCAATAGATTACTTCTGCCAAAGTTCATGGGGAAAGGATTTTGTAGAAAAACAAACGATTAACATCTTTTCAATTTTTGATACACGAAGTGGATCACCACAGAGAGATATTTCTTAACTCCCTAGGATGTGCGTTCATATGAAAATTCACTTTTCTCGTATACCTTTGATGCCAAAACTCCTCTTGATGTTCGAAGGAATAACAACCTCCTACCACCATCAAAACGTAGAGTAGTTCTAAATTACCTCACTAATATTTGAAGGAACTGATACCTCCACGTTCATCTCCACTGCAACAATTTTCTTTGTATGAGTTCAACATGTGAAGGTAGAGATTCCTAGGATGTAATGTCTTAATCAGTTGGGCTCTATGCACAAGTTGGCTTACTTCAACCGCTTCCAACCACTTAAGATGGATTTATTCTTGCAGTTGCCTGGAACCTGAGAAAAGTACAATGCCATGCTAATTTCATCTTCCAAATTTTAATATCTCCACTATTCTCTATAACTGGTAATTGAATGCTTATTCCAGTGCCGTGCTAATTACAGTGCAACTATAACAGCTCAGGGAGGCCATTGGCCTTCACAGCTAAATGTAAAGCATTTAGTTACCAGTTATAGAAAATAGTGGAGATCAAAATTTGGAAGCTACAATTAGCACGCGTTGTGCTTTTCCAAGGTTAAATGCAACTGCAAGATTAAATCCATCCCAATACCTTTACCTTGGAACTATTACATTATTTGCTTCTACTCCTTCATTACCCGCAAGAATGTTTCTCATTTGTGTATTTAATAGAGAACTTCCAATTCCTAAGGAAATTGAGGATCCAAGTGAGGAAACTAGTTCAGTTGAGAACAAGGAGCTAGCTTCACAATTTCAAATTCCTGACCCTACCTTCTCTGTTTCTCGTCAAGTACACGTGAAATTGTCAATGCTAAACCAAGTATCTACGTTCTTGGTATTTCCTACCTATGAGTGCATCATCTTAAACGCCTAGTCCCACAGACATGATGGTGTCTGTGCATGGCCGTTTTTCTGTCGGTTTTGAAGGTTTCCCTTGACCATTTGAAAAGAGCTTTCTTTTCATTTTATTTTCCTTGAAAATGAGCTTGTTCAAGAGATAAAATCCGAAGTGTATAAAACCATAATCCCTAAATAATAAACCTGTAGCTTCCAAAAGGAAGCTAGAAACCCCTCAATGGTTAATCGAATCCACACATTGAAAAAACTGAATTAGGATCATCGTTTTTCAACTGCCTTGTATTGGATGTCTTTTGGTGTTTGATGAGGCATTTATCATGTTTACTGGCAGTGGAGAGTTTTGTAAACCAAGTAAACTCAGCAACTGAATGGTGATTTGAGAGTTTCTTGGTTGGGCAACTAAAATAATGCCAGAAGAAAGTTTTGATTTATGAAGTGAACTTGGGTTTAATTCAATATCTTATTGAAGTACTCATGTTCTAATTTAGAGCATGCTTGTGATGCATCATACAGTATTCAATGTATGAAGAATACCAACTACAAGATATAATCTGTGACCTTTCTCATTAAATTAATAACATAACAGCTTCTGACAATAATGATTGTTTTTCATTCCATTTGTAACAAAAACAGCCTGGCTGCATATATGACTGAAGATGAAGTCCACGAAGGAATTATTTATCCGTCAATTTCTAGGTAAATTCATCATCTTTATATGATGTTTAAATACTTCTATTTGATTGAGATGAGAAAGTGGGAAATGTTTCATGAGCATGTTTTTGTTTGTAGCATCCGTGATATTACAAAGGAAATTGCGGCGGCTGTTATTATGGAAGCCATAGAAGAGGATCTTGTCGAAGGATACCGTGGCGTCGATGCTCGAGAACTCCGAAAATTTAGCAAGGTAGATTCTATTGTTAGAAACTTTCCTAATCTTGTTTCTAGTGATTTTTGCATTTTGTTCGTTAAACAATTGCAATAACCCAACCTAAACTTAGTTGAATTGATTAAAACATTTACTATCGATTAAGAGGTTAAAGATTTAAGTCTCTCACCCCACATTTTGAACTAGAAGAGAGGATCAAACATACATCTACTTATAGAATAATAATTGGTGTCTTATCCATTGAACTATGCTTAAGTTCCATAGGGTGTAATGACTTGTTTGTCGTATGATTTTTATAACGTAATGCAAATTATAAGTCGATAACATCGTCAAGGAATAATCCAATTTTCATGTTTTCTTTTCTTCCAGGAGGAAATTTTGGAATTTGTGAAGAACAACATGTGGAGTCCAGAATATCCGACACTAGTTTATAGTCAGGATTGA

mRNA sequence

ATGACGAAGTGGAATTCTCTTTCTGCTTCGCTTATGAAGCGTCTCAAGCTTCACTACCAGATGACCCACAATATTCCAGCGTTAGCTCAGGCGAGATCCTTCACCACAAGCGAGGGGCATCGTCCCACCATTGTTCATAAACGTAGCCTCGATATTCTTCACGATCCGTGGTTTAACAAAGGAACTGCCTTTACGATGACAGAACGTGACCGGCTTGATCTTCGAGGACTTCTTCCACCAAATGTAATGTCTTCAGAGCAGCAAATTGAACGCTTTATGGTTGACCTGAAGAGGCTTGAAGTGCAAGCAAGAGATGGGCCTTCAGATCCTAATGCTTTGGCCAAATGGCGAATTCTGAATAGATTGCACGATAGAAACGAGACAATGTACTATAAAGTTTTGATTGCTCACATTGAGGAGTATGCACCTATAGTATATACACCAACTGTTGGTCTTGTTTGCCAGAACTATAGTGGCTTATTTAGAAGACCAAGGGGAATGTATTTTAGTGCAGAAGATCGAGGAGAAATGATGTCCATGGTTTATAATTGGCCTGCTGATCAGGTTGATATGATTGTTGTTACTGATGGAAGTAGAATATTAGGTCTTGGAGATCTCGGAGTTCACGGGATTGGGATTGCCATCGGAAAGTTGGATTTATATGTTGCTGCTGCTGGGATAAATCCTCAGAGGGTGCTTCCAGTCATGATTGATGTTGGAACCAACAATGAAAAGCTTCTGAAGGATCCCTTATATTTAGGATTGCAAGAACACCGATTGGATGGTGATGAATATTTGGCCATTATCGATGAATTCATGGAGGCTGTGTTTACTCGCTGGCCACATGTTATTGTTCAATTTGAAGATTTCCAAAGCAAATGGGCATTTAAGTTATTGCAACGATACAGAAATAACTACAGAATGTTTAATGATGACGTTCAGGGAACGGCAGGAGTTGCTATTGCTGGACTTTTAGGGGCTGTGAGAGCACAAGGAAGACCGATGATTGACTTTCCAAAGCAAAAGATTGTTGTTGCTGGTGCTGGAAGTGCAGGAATTGGGGTTTTAAACGCGGCAAGGAAAACCATGGCGAGGATGTTGGGAAACAATGAATCAGCATTCGAGGCTGCCCGAAGCCAATTTTGGGTGGTAGATGCCCAGGGTTTGATCACAGAGGAAAGAGAAAATATTGATCAAGATGCAAGTCCGTTTGCAAGGAAGGTCAAAGAAATTAATCGTCAAGGATTGAGGGAAGGTGCAAGTCTAGTGGAAGTGGTGCAACAAGTGAAACCTGATGTGCTTCTGGGATTGTCTGCCGTTGGTGGATTGTTCACAAAGGAGGTGTTGGAGGCTCTTAAAGGATCAACAGCTACAAGACCAGCTATTTTTGCCATGTCTAATCCTACAACAAATGCCGAGTGCACTCCTCAAGAAGCATTCTCAATCTTGGGTGAAAACATCATATTTGCAAGTGGAAGCCCATTCAAAGATGTCGATTTTGGGAATGGTCACATTGGCCACTGCAACCAAGGAAACAACATGTATCTTTTCCCAGGTATTGGGCTCGGTACGCTTCTATCTGGCTCTCCTATCGTATCTGATGGCATGCTGCAGGCTGCTGCTGAGTGCCTGGCTGCATATATGACTGAAGATGAAGTCCACGAAGGAATTATTTATCCGTCAATTTCTAGCATCCGTGATATTACAAAGGAAATTGCGGCGGCTGTTATTATGGAAGCCATAGAAGAGGATCTTGTCGAAGGATACCGTGGCGTCGATGCTCGAGAACTCCGAAAATTTAGCAAGGAGGAAATTTTGGAATTTGTGAAGAACAACATGTGGAGTCCAGAATATCCGACACTAGTTTATAGTCAGGATTGA

Coding sequence (CDS)

ATGACGAAGTGGAATTCTCTTTCTGCTTCGCTTATGAAGCGTCTCAAGCTTCACTACCAGATGACCCACAATATTCCAGCGTTAGCTCAGGCGAGATCCTTCACCACAAGCGAGGGGCATCGTCCCACCATTGTTCATAAACGTAGCCTCGATATTCTTCACGATCCGTGGTTTAACAAAGGAACTGCCTTTACGATGACAGAACGTGACCGGCTTGATCTTCGAGGACTTCTTCCACCAAATGTAATGTCTTCAGAGCAGCAAATTGAACGCTTTATGGTTGACCTGAAGAGGCTTGAAGTGCAAGCAAGAGATGGGCCTTCAGATCCTAATGCTTTGGCCAAATGGCGAATTCTGAATAGATTGCACGATAGAAACGAGACAATGTACTATAAAGTTTTGATTGCTCACATTGAGGAGTATGCACCTATAGTATATACACCAACTGTTGGTCTTGTTTGCCAGAACTATAGTGGCTTATTTAGAAGACCAAGGGGAATGTATTTTAGTGCAGAAGATCGAGGAGAAATGATGTCCATGGTTTATAATTGGCCTGCTGATCAGGTTGATATGATTGTTGTTACTGATGGAAGTAGAATATTAGGTCTTGGAGATCTCGGAGTTCACGGGATTGGGATTGCCATCGGAAAGTTGGATTTATATGTTGCTGCTGCTGGGATAAATCCTCAGAGGGTGCTTCCAGTCATGATTGATGTTGGAACCAACAATGAAAAGCTTCTGAAGGATCCCTTATATTTAGGATTGCAAGAACACCGATTGGATGGTGATGAATATTTGGCCATTATCGATGAATTCATGGAGGCTGTGTTTACTCGCTGGCCACATGTTATTGTTCAATTTGAAGATTTCCAAAGCAAATGGGCATTTAAGTTATTGCAACGATACAGAAATAACTACAGAATGTTTAATGATGACGTTCAGGGAACGGCAGGAGTTGCTATTGCTGGACTTTTAGGGGCTGTGAGAGCACAAGGAAGACCGATGATTGACTTTCCAAAGCAAAAGATTGTTGTTGCTGGTGCTGGAAGTGCAGGAATTGGGGTTTTAAACGCGGCAAGGAAAACCATGGCGAGGATGTTGGGAAACAATGAATCAGCATTCGAGGCTGCCCGAAGCCAATTTTGGGTGGTAGATGCCCAGGGTTTGATCACAGAGGAAAGAGAAAATATTGATCAAGATGCAAGTCCGTTTGCAAGGAAGGTCAAAGAAATTAATCGTCAAGGATTGAGGGAAGGTGCAAGTCTAGTGGAAGTGGTGCAACAAGTGAAACCTGATGTGCTTCTGGGATTGTCTGCCGTTGGTGGATTGTTCACAAAGGAGGTGTTGGAGGCTCTTAAAGGATCAACAGCTACAAGACCAGCTATTTTTGCCATGTCTAATCCTACAACAAATGCCGAGTGCACTCCTCAAGAAGCATTCTCAATCTTGGGTGAAAACATCATATTTGCAAGTGGAAGCCCATTCAAAGATGTCGATTTTGGGAATGGTCACATTGGCCACTGCAACCAAGGAAACAACATGTATCTTTTCCCAGGTATTGGGCTCGGTACGCTTCTATCTGGCTCTCCTATCGTATCTGATGGCATGCTGCAGGCTGCTGCTGAGTGCCTGGCTGCATATATGACTGAAGATGAAGTCCACGAAGGAATTATTTATCCGTCAATTTCTAGCATCCGTGATATTACAAAGGAAATTGCGGCGGCTGTTATTATGGAAGCCATAGAAGAGGATCTTGTCGAAGGATACCGTGGCGTCGATGCTCGAGAACTCCGAAAATTTAGCAAGGAGGAAATTTTGGAATTTGTGAAGAACAACATGTGGAGTCCAGAATATCCGACACTAGTTTATAGTCAGGATTGA

Protein sequence

MTKWNSLSASLMKRLKLHYQMTHNIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNKGTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILNRLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEKLLKDPLYLGLQEHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNNYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAARKTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGASLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPQEAFSILGENIIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAAAECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKFSKEEILEFVKNNMWSPEYPTLVYSQD
Homology
BLAST of CmUC05G083350 vs. NCBI nr
Match: XP_008438266.1 (PREDICTED: NAD-dependent malic enzyme 62 kDa isoform, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 1232.2 bits (3187), Expect = 0.0e+00
Identity = 615/626 (98.24%), Postives = 625/626 (99.84%), Query Frame = 0

Query: 1   MTKWNSLSASLMKRLKLHYQMTHNIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK 60
           MTKWNSLSASLMKRLKLHYQMT++IPALAQ+RSFTTSEGHRPTIVHKRSLDILHDPWFNK
Sbjct: 1   MTKWNSLSASLMKRLKLHYQMTNSIPALAQSRSFTTSEGHRPTIVHKRSLDILHDPWFNK 60

Query: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120
           GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN
Sbjct: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120

Query: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180
           RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM
Sbjct: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180

Query: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240
           VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG
Sbjct: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240

Query: 241 TNNEKLLKDPLYLGLQEHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300
           TNNEKLLKDPLYLGLQ+HRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ
Sbjct: 241 TNNEKLLKDPLYLGLQQHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300

Query: 301 RYRNNYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360
           RYRN YRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR
Sbjct: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360

Query: 361 KTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420
           KTMARMLGNNE+AFEAARSQFWVVDAQGLITEEREN+DQDASPFARKVKEINRQGLREGA
Sbjct: 361 KTMARMLGNNEAAFEAARSQFWVVDAQGLITEERENLDQDASPFARKVKEINRQGLREGA 420

Query: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPQEAF 480
           SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTP+EAF
Sbjct: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAF 480

Query: 481 SILGENIIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540
           SILGE++IFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA
Sbjct: 481 SILGESVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540

Query: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKF 600
           AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKF
Sbjct: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKF 600

Query: 601 SKEEILEFVKNNMWSPEYPTLVYSQD 627
           SKEEILEFVKNNMWSPEYPTLVY+QD
Sbjct: 601 SKEEILEFVKNNMWSPEYPTLVYNQD 626

BLAST of CmUC05G083350 vs. NCBI nr
Match: XP_004133957.1 (NAD-dependent malic enzyme 1, mitochondrial isoform X1 [Cucumis sativus] >KGN56672.1 hypothetical protein Csa_011430 [Cucumis sativus])

HSP 1 Score: 1229.5 bits (3180), Expect = 0.0e+00
Identity = 614/626 (98.08%), Postives = 624/626 (99.68%), Query Frame = 0

Query: 1   MTKWNSLSASLMKRLKLHYQMTHNIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK 60
           MTKWNSLSASLMKRLKLHYQM ++IPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK
Sbjct: 1   MTKWNSLSASLMKRLKLHYQMINSIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK 60

Query: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120
           GTAFT+TERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN
Sbjct: 61  GTAFTITERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120

Query: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180
           RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSA+DRGEMMSM
Sbjct: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAQDRGEMMSM 180

Query: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240
           VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG
Sbjct: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240

Query: 241 TNNEKLLKDPLYLGLQEHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300
           TNNEKLLKDPLYLGLQ+HRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ
Sbjct: 241 TNNEKLLKDPLYLGLQQHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300

Query: 301 RYRNNYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360
           RYRN YRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR
Sbjct: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360

Query: 361 KTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420
           KTMARMLGNNE+AFEAARSQFWVVDAQGLITEER+NIDQDASPFARKVKEINRQGLREGA
Sbjct: 361 KTMARMLGNNEAAFEAARSQFWVVDAQGLITEERKNIDQDASPFARKVKEINRQGLREGA 420

Query: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPQEAF 480
           SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTP+EAF
Sbjct: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAF 480

Query: 481 SILGENIIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540
           SILGEN+IFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA
Sbjct: 481 SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540

Query: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKF 600
           AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKF
Sbjct: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKF 600

Query: 601 SKEEILEFVKNNMWSPEYPTLVYSQD 627
           SKEEILEFVKNNMWSPEYPTLVY+QD
Sbjct: 601 SKEEILEFVKNNMWSPEYPTLVYNQD 626

BLAST of CmUC05G083350 vs. NCBI nr
Match: XP_023528517.1 (NAD-dependent malic enzyme 1, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo] >KAG6582518.1 NAD-dependent malic enzyme 1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1227.2 bits (3174), Expect = 0.0e+00
Identity = 612/626 (97.76%), Postives = 624/626 (99.68%), Query Frame = 0

Query: 1   MTKWNSLSASLMKRLKLHYQMTHNIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK 60
           MTKWNSLSASLMKRLKLHYQM+++IPALAQARSFTTSEGHRP I+HKRSLDILHDPWFNK
Sbjct: 1   MTKWNSLSASLMKRLKLHYQMSNSIPALAQARSFTTSEGHRPAIIHKRSLDILHDPWFNK 60

Query: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120
           GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN
Sbjct: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120

Query: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180
           RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM
Sbjct: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180

Query: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240
           VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG
Sbjct: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240

Query: 241 TNNEKLLKDPLYLGLQEHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300
           TNNEKLLKDPLYLGLQ++RLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ
Sbjct: 241 TNNEKLLKDPLYLGLQQNRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300

Query: 301 RYRNNYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360
           RYRN YRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR
Sbjct: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360

Query: 361 KTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420
           KTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA
Sbjct: 361 KTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420

Query: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPQEAF 480
           SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTP++AF
Sbjct: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEQAF 480

Query: 481 SILGENIIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540
           SILGEN+IFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSG+PIVSDGMLQAA
Sbjct: 481 SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGAPIVSDGMLQAA 540

Query: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKF 600
           AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKF
Sbjct: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKF 600

Query: 601 SKEEILEFVKNNMWSPEYPTLVYSQD 627
           SKE+ILEFVKNNMWSPEYPTLVY+QD
Sbjct: 601 SKEQILEFVKNNMWSPEYPTLVYNQD 626

BLAST of CmUC05G083350 vs. NCBI nr
Match: XP_022980252.1 (NAD-dependent malic enzyme 1, mitochondrial isoform X1 [Cucurbita maxima])

HSP 1 Score: 1226.1 bits (3171), Expect = 0.0e+00
Identity = 611/626 (97.60%), Postives = 624/626 (99.68%), Query Frame = 0

Query: 1   MTKWNSLSASLMKRLKLHYQMTHNIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK 60
           MTKWNSLSASLMKRLKLHYQM+++IPALAQARSFTTSEGHRP I+HKRSLDILHDPWFNK
Sbjct: 1   MTKWNSLSASLMKRLKLHYQMSNSIPALAQARSFTTSEGHRPAIIHKRSLDILHDPWFNK 60

Query: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120
           GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN
Sbjct: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120

Query: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180
           RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM
Sbjct: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180

Query: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240
           VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG
Sbjct: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240

Query: 241 TNNEKLLKDPLYLGLQEHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300
           TNNEKLLKDPLYLGLQ++RLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ
Sbjct: 241 TNNEKLLKDPLYLGLQQNRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300

Query: 301 RYRNNYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360
           RYRN YRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR
Sbjct: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360

Query: 361 KTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420
           KTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA
Sbjct: 361 KTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420

Query: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPQEAF 480
           SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTP++AF
Sbjct: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEQAF 480

Query: 481 SILGENIIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540
           SILGEN+IFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSG+PIVSDGMLQAA
Sbjct: 481 SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGAPIVSDGMLQAA 540

Query: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKF 600
           AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVD+RELRKF
Sbjct: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDSRELRKF 600

Query: 601 SKEEILEFVKNNMWSPEYPTLVYSQD 627
           SKE+ILEFVKNNMWSPEYPTLVY+QD
Sbjct: 601 SKEQILEFVKNNMWSPEYPTLVYNQD 626

BLAST of CmUC05G083350 vs. NCBI nr
Match: KAG7018901.1 (NAD-dependent malic enzyme 1, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1225.7 bits (3170), Expect = 0.0e+00
Identity = 611/626 (97.60%), Postives = 624/626 (99.68%), Query Frame = 0

Query: 1   MTKWNSLSASLMKRLKLHYQMTHNIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK 60
           MTKWNSLSASLMKRLKLHYQM+++IPALAQARSFTTSEGHRP I+HKRSLDILHDPWFNK
Sbjct: 1   MTKWNSLSASLMKRLKLHYQMSNSIPALAQARSFTTSEGHRPAIIHKRSLDILHDPWFNK 60

Query: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120
           GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN
Sbjct: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120

Query: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180
           RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM
Sbjct: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180

Query: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240
           VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG
Sbjct: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240

Query: 241 TNNEKLLKDPLYLGLQEHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300
           TNNEKLLKDPLYLGLQ++RLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ
Sbjct: 241 TNNEKLLKDPLYLGLQQNRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300

Query: 301 RYRNNYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360
           RYRN YRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR
Sbjct: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360

Query: 361 KTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420
           KTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA
Sbjct: 361 KTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420

Query: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPQEAF 480
           SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTP++AF
Sbjct: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEQAF 480

Query: 481 SILGENIIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540
           SILGEN+IFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSG+PIVSDGMLQAA
Sbjct: 481 SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGAPIVSDGMLQAA 540

Query: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKF 600
           AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDAREL+KF
Sbjct: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELQKF 600

Query: 601 SKEEILEFVKNNMWSPEYPTLVYSQD 627
           SKE+ILEFVKNNMWSPEYPTLVY+QD
Sbjct: 601 SKEQILEFVKNNMWSPEYPTLVYNQD 626

BLAST of CmUC05G083350 vs. ExPASy Swiss-Prot
Match: Q9SIU0 (NAD-dependent malic enzyme 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NAD-ME1 PE=1 SV=1)

HSP 1 Score: 1063.5 bits (2749), Expect = 9.0e-310
Identity = 526/620 (84.84%), Postives = 577/620 (93.06%), Query Frame = 0

Query: 7   LSASLMKRLKLHYQMTHNIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNKGTAFTM 66
           LS+S + R+ LH ++ ++    +  RSFTTSEGHRPTIVHK+ LDILHDPWFNKGTAFTM
Sbjct: 9   LSSSSLSRI-LHRRILYS----SAVRSFTTSEGHRPTIVHKQGLDILHDPWFNKGTAFTM 68

Query: 67  TERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILNRLHDRN 126
           TER+RLDLRGLLPPNVM SEQQI RFM DLKRLE QARDGPSDPNALAKWRILNRLHDRN
Sbjct: 69  TERNRLDLRGLLPPNVMDSEQQIFRFMTDLKRLEEQARDGPSDPNALAKWRILNRLHDRN 128

Query: 127 ETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPA 186
           ETMYYKVLI +IEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPA
Sbjct: 129 ETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPA 188

Query: 187 DQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEKL 246
           +QVDMIVVTDGSRILGLGDLGVHGIGIA+GKLDLYVAAAGINPQRVLPVMIDVGTNNEKL
Sbjct: 189 EQVDMIVVTDGSRILGLGDLGVHGIGIAVGKLDLYVAAAGINPQRVLPVMIDVGTNNEKL 248

Query: 247 LKDPLYLGLQEHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNNY 306
             DP+YLGLQ+ RL+ D+Y+ +IDEFMEAV+TRWPHVIVQFEDFQSKWAFKLLQRYR  Y
Sbjct: 249 RNDPMYLGLQQRRLEDDDYIDVIDEFMEAVYTRWPHVIVQFEDFQSKWAFKLLQRYRCTY 308

Query: 307 RMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAARKTMARM 366
           RMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPK KIVVAGAGSAGIGVLNAARKTMARM
Sbjct: 309 RMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKMKIVVAGAGSAGIGVLNAARKTMARM 368

Query: 367 LGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGASLVEVV 426
           LGN E+AF++A+SQFWVVDAQGLITE RENID +A PFARK KE+ RQGL+EGA+LVEVV
Sbjct: 369 LGNTETAFDSAQSQFWVVDAQGLITEGRENIDPEAQPFARKTKEMERQGLKEGATLVEVV 428

Query: 427 QQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPQEAFSILGEN 486
           ++VKPDVLLGLSAVGGLF+KEVLEA+KGST+TRPAIFAMSNPT NAECTPQ+AFSILGEN
Sbjct: 429 REVKPDVLLGLSAVGGLFSKEVLEAMKGSTSTRPAIFAMSNPTKNAECTPQDAFSILGEN 488

Query: 487 IIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAAAECLAA 546
           +IFASGSPFK+V+FGNGH+GHCNQGNNMYLFPGIGLGTLLSG+PIVSDGMLQAA+ECLAA
Sbjct: 489 MIFASGSPFKNVEFGNGHVGHCNQGNNMYLFPGIGLGTLLSGAPIVSDGMLQAASECLAA 548

Query: 547 YMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKFSKEEIL 606
           YM+E+EV EGIIYP IS IRDITK IAAAVI EAIEEDLVEGYR +DARE++K  +E ++
Sbjct: 549 YMSEEEVLEGIIYPPISRIRDITKRIAAAVIKEAIEEDLVEGYREMDAREIQKLDEEGLM 608

Query: 607 EFVKNNMWSPEYPTLVYSQD 627
           E+V+NNMW+PEYPTLVY  D
Sbjct: 609 EYVENNMWNPEYPTLVYKDD 623

BLAST of CmUC05G083350 vs. ExPASy Swiss-Prot
Match: P37221 (NAD-dependent malic enzyme 62 kDa isoform, mitochondrial OS=Solanum tuberosum OX=4113 PE=1 SV=1)

HSP 1 Score: 1062.4 bits (2746), Expect = 2.0e-309
Identity = 526/620 (84.84%), Postives = 573/620 (92.42%), Query Frame = 0

Query: 7   LSASLMKRLKLHYQMTHNIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNKGTAFTM 66
           LS++L+KR  LH ++   + + + +R+FTT+EGHRPTIVHKRSLDILHDPWFNKGTAF+ 
Sbjct: 10  LSSTLLKR--LHQRVAAAVNS-SSSRNFTTTEGHRPTIVHKRSLDILHDPWFNKGTAFSF 69

Query: 67  TERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILNRLHDRN 126
           TERDRL +RGLLPPNVMS EQQI RFM DLKRLEVQARDGPSDP  LAKWRILNRLHDRN
Sbjct: 70  TERDRLHIRGLLPPNVMSFEQQIARFMADLKRLEVQARDGPSDPYVLAKWRILNRLHDRN 129

Query: 127 ETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPA 186
           ET+YYKVL+ +IEEYAPIVYTPTVGLVCQ YSGLFRRPRGMYFSAEDRGEMMSMVYNWPA
Sbjct: 130 ETLYYKVLMENIEEYAPIVYTPTVGLVCQKYSGLFRRPRGMYFSAEDRGEMMSMVYNWPA 189

Query: 187 DQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEKL 246
           DQVDMIVVTDGSRILGLGDLG+ GIGIAIGKLDLYVAAAGINPQRVLPVMIDVGT+NE L
Sbjct: 190 DQVDMIVVTDGSRILGLGDLGIQGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTDNENL 249

Query: 247 LKDPLYLGLQEHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNNY 306
           LKDPLYLGLQ+HRLDG+EY+ +IDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNNY
Sbjct: 250 LKDPLYLGLQDHRLDGEEYIEVIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNNY 309

Query: 307 RMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAARKTMARM 366
           RMFNDD+QGTAGVAIAGLLGAVRAQGRPMIDFPK KIVVAGAGSAGIGVLNAARKTMARM
Sbjct: 310 RMFNDDIQGTAGVAIAGLLGAVRAQGRPMIDFPKMKIVVAGAGSAGIGVLNAARKTMARM 369

Query: 367 LGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGASLVEVV 426
           LGN E AFE+ARSQFWVVDA+GLITE REN+D DA PFARK+KEI RQGL EGA+L EVV
Sbjct: 370 LGNTEIAFESARSQFWVVDAKGLITEARENVDPDARPFARKIKEIERQGLSEGATLAEVV 429

Query: 427 QQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPQEAFSILGEN 486
           ++VKPDVLLGLSA GGLF+KEVLEALK ST+TRPAIF MSNPT NAECTP+EAFSILGEN
Sbjct: 430 REVKPDVLLGLSACGGLFSKEVLEALKHSTSTRPAIFPMSNPTRNAECTPEEAFSILGEN 489

Query: 487 IIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAAAECLAA 546
           IIFASGSPFKDVD GNGH+GHCNQ NNM+LFPGIGLGTLLSGS IVSDGMLQAAAECLAA
Sbjct: 490 IIFASGSPFKDVDLGNGHVGHCNQANNMFLFPGIGLGTLLSGSRIVSDGMLQAAAECLAA 549

Query: 547 YMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKFSKEEIL 606
           Y+TE+EV +GIIYPSIS IRDITKE+AAAV+ EAIEEDL EGYR +D+RELRK  + +I 
Sbjct: 550 YITEEEVLKGIIYPSISRIRDITKEVAAAVVKEAIEEDLAEGYREMDSRELRKLDEAQIS 609

Query: 607 EFVKNNMWSPEYPTLVYSQD 627
           EFV+NNMWSP+YPTLVY +D
Sbjct: 610 EFVENNMWSPDYPTLVYKKD 626

BLAST of CmUC05G083350 vs. ExPASy Swiss-Prot
Match: P37224 (NAD-dependent malic enzyme 65 kDa isoform, mitochondrial OS=Amaranthus hypochondriacus OX=28502 PE=1 SV=1)

HSP 1 Score: 977.2 bits (2525), Expect = 8.5e-284
Identity = 485/619 (78.35%), Postives = 551/619 (89.01%), Query Frame = 0

Query: 7   LSASLMKRLKLHYQMTHNIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNKGTAFTM 66
           L++SL++RLK        I  ++  RSF TSEGHR  IV+KRSLDIL DPWFNKGTAF+M
Sbjct: 10  LTSSLIRRLK------DQIANVSNHRSFATSEGHRLAIVNKRSLDILQDPWFNKGTAFSM 69

Query: 67  TERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILNRLHDRN 126
           TERDRLDLRGLLPPNVM++EQQIERF  DL+ LE+  +DGPSD   LAKWRILNRLHDRN
Sbjct: 70  TERDRLDLRGLLPPNVMTTEQQIERFTADLRVLELTTKDGPSDTYDLAKWRILNRLHDRN 129

Query: 127 ETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPA 186
           ETM++KVLI +IEEYAPIV TPTVGLVCQ +SGL+RRPRGMYFS++DRGEMMSMVYNWPA
Sbjct: 130 ETMFFKVLIENIEEYAPIVSTPTVGLVCQKFSGLYRRPRGMYFSSDDRGEMMSMVYNWPA 189

Query: 187 DQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEKL 246
           +QVDMIVVTDGSRILGLGDLGVHGIG+AIGKLDLYVAAAGINPQRVLPVMIDVGTNNE L
Sbjct: 190 EQVDMIVVTDGSRILGLGDLGVHGIGVAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEDL 249

Query: 247 LKDPLYLGLQEHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNNY 306
           LK+PLYLGLQ+ RLDG+EYLA++DEFMEAVFTRWP+VIVQFED Q+KWA  LLQRYR+ Y
Sbjct: 250 LKNPLYLGLQKKRLDGEEYLAVMDEFMEAVFTRWPNVIVQFEDIQNKWALTLLQRYRHKY 309

Query: 307 RMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAARKTMARM 366
           R FN DVQGT+GVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGS+G+GVLNAARKTMARM
Sbjct: 310 RTFNVDVQGTSGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSSGVGVLNAARKTMARM 369

Query: 367 LGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGASLVEVV 426
           LGN+ESAF+ ARSQFWVVD +GLITE+R N+D +  PFA K  EI+ QGL EGA LVEVV
Sbjct: 370 LGNDESAFDRARSQFWVVDDKGLITEKRANLDPEVQPFAWKENEISLQGLNEGAKLVEVV 429

Query: 427 QQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPQEAFSILGEN 486
           +QVKPDVLLGLSA GGLF+KEVLEALK ST+TRPAIFAMSNPT NAECTP+EAFSI+G++
Sbjct: 430 RQVKPDVLLGLSAYGGLFSKEVLEALKDSTSTRPAIFAMSNPTKNAECTPEEAFSIVGDH 489

Query: 487 IIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAAAECLAA 546
           +++ASGSPFKDVD GNG IGH NQGNNMYLFPGIGLG LLSGS I+SD M QAAAE LA 
Sbjct: 490 VVYASGSPFKDVDLGNGKIGHVNQGNNMYLFPGIGLGVLLSGSRIISDSMFQAAAERLAG 549

Query: 547 YMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKFSKEEIL 606
           YMT++EV  G+IYPSIS IRDITKE+AAAVI EA+EEDL EGYR +DAREL+K ++E+IL
Sbjct: 550 YMTDEEVINGVIYPSISRIRDITKEVAAAVIKEAVEEDLAEGYRDMDARELQKLNEEQIL 609

Query: 607 EFVKNNMWSPEYPTLVYSQ 626
           E+++ NMW+PEYPTLVY +
Sbjct: 610 EYIEKNMWNPEYPTLVYKK 622

BLAST of CmUC05G083350 vs. ExPASy Swiss-Prot
Match: P37225 (NAD-dependent malic enzyme 59 kDa isoform, mitochondrial OS=Solanum tuberosum OX=4113 PE=1 SV=1)

HSP 1 Score: 796.2 bits (2055), Expect = 2.7e-229
Identity = 390/582 (67.01%), Postives = 468/582 (80.41%), Query Frame = 0

Query: 42  PTIVHKRSLDILHDPWFNKGTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEV 101
           P IVHKR  DILHDPWFNK T F MTERDRL LRGLLPP V+S EQQ +RFM   + LE 
Sbjct: 25  PCIVHKRGADILHDPWFNKDTGFPMTERDRLGLRGLLPPRVISFEQQYDRFMESFRSLEK 84

Query: 102 QARDGPSDPNALAKWRILNRLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLF 161
                P    +LAKWRILNRLHDRNET+YY+VLI +I+++API+YTPTVGLVCQNYSGLF
Sbjct: 85  NTEGQPDSVVSLAKWRILNRLHDRNETLYYRVLIDNIKDFAPIIYTPTVGLVCQNYSGLF 144

Query: 162 RRPRGMYFSAEDRGEMMSMVYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLY 221
           RRPRGMYFSA+D+GEMMSM++NWP+ QVDMIV+TDGSRILGLGDLGV GIGI IGKLD+Y
Sbjct: 145 RRPRGMYFSAKDKGEMMSMIFNWPSTQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDMY 204

Query: 222 VAAAGINPQRVLPVMIDVGTNNEKLLKDPLYLGLQEHRLDGDEYLAIIDEFMEAVFTRWP 281
           VAAAGINPQRVLPVM+DVGTNN+KLL+DPLYLGL++ RL+G+EYL+I+DEF+EAV  RWP
Sbjct: 205 VAAAGINPQRVLPVMLDVGTNNQKLLEDPLYLGLRQPRLEGEEYLSIVDEFVEAVHARWP 264

Query: 282 HVIVQFEDFQSKWAFKLLQRYRNNYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQ 341
             +VQFEDFQ+KWAF+ L RYR  + MFNDD+QGTAGVA+AGLLG VRAQGRP+ DF  Q
Sbjct: 265 KAVVQFEDFQAKWAFETLDRYRKKFCMFNDDIQGTAGVALAGLLGTVRAQGRPLTDFANQ 324

Query: 342 KIVVAGAGSAGIGVLNAARKTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDA 401
           KIVV GAGSAG+GVL  A + ++RM G       +A   F+++D  GLIT++R++ID  A
Sbjct: 325 KIVVVGAGSAGLGVLKMALQAVSRMTG------PSADPHFFLLDKNGLITKDRKDIDPAA 384

Query: 402 SPFARKVKEINRQGLREGASLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPA 461
            PFA+   EI   GL+EGA L EVV++VKP VLLGLS VGG+F +EVL A+K S + RPA
Sbjct: 385 LPFAKAHHEIEGLGLQEGAGLAEVVKKVKPHVLLGLSGVGGIFHEEVLRAMKESDSVRPA 444

Query: 462 IFAMSNPTTNAECTPQEAFSILGENIIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIG 521
           IFAMSNPT NAEC P +AF + GE+I+FASGSPF +VD GNG IGH NQ NNMYLFPGIG
Sbjct: 445 IFAMSNPTNNAECCPVDAFKLAGEDIVFASGSPFANVDLGNGKIGHVNQANNMYLFPGIG 504

Query: 522 LGTLLSGSPIVSDGMLQAAAECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAI 581
           LG LLSG+  +SD ML+AAAECLA+YM++DE++ GI+YPSI  IRDIT E+ AAV+  A+
Sbjct: 505 LGALLSGARNISDTMLEAAAECLASYMSDDEINRGILYPSIDDIRDITAEVGAAVLRAAV 564

Query: 582 EEDLVEGYRGVDARELRKFSKEEILEFVKNNMWSPEYPTLVY 624
            EDL EG+  V  +EL+  SKEE +E V+ NMW P Y  LV+
Sbjct: 565 AEDLAEGHGDVGVKELQHMSKEETIEHVRQNMWYPVYGPLVH 600

BLAST of CmUC05G083350 vs. ExPASy Swiss-Prot
Match: Q8L7K9 (NAD-dependent malic enzyme 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NAD-ME2 PE=1 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 5.8e-224
Identity = 386/614 (62.87%), Postives = 478/614 (77.85%), Query Frame = 0

Query: 21  MTHNIPALAQARSFTTSEGHR---------PTIVHKRSLDILHDPWFNKGTAFTMTERDR 80
           M  NI  L++A +   + G R         P IVHKR  DILHDPWFNK T F +TERDR
Sbjct: 2   MWKNIAGLSKAAAAARTHGSRRCFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDR 61

Query: 81  LDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILNRLHDRNETMYY 140
           L +RGLLPP VM+  QQ +RF+   + LE   +  P +  ALAKWR+LNRLHDRNET+YY
Sbjct: 62  LGIRGLLPPRVMTCVQQCDRFIESFRSLENNTKGEPENVVALAKWRMLNRLHDRNETLYY 121

Query: 141 KVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPADQVDM 200
           +VLI +I+++API+YTPTVGLVCQNYSGL+RRPRGMYFSA+D+GEMMSM+YNWPA QVDM
Sbjct: 122 RVLIDNIKDFAPIIYTPTVGLVCQNYSGLYRRPRGMYFSAKDKGEMMSMIYNWPAPQVDM 181

Query: 201 IVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEKLLKDPL 260
           IV+TDGSRILGLGDLGV GIGI IGKLD+YVAAAGINPQRVLP+M+DVGTNNEKLL++ L
Sbjct: 182 IVITDGSRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRVLPIMLDVGTNNEKLLQNDL 241

Query: 261 YLGLQEHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNNYRMFND 320
           YLG+++ RL+G+EYL IIDEFMEA FTRWP  +VQFEDFQ+KWAF  L+RYR  + MFND
Sbjct: 242 YLGVRQPRLEGEEYLEIIDEFMEAAFTRWPKAVVQFEDFQAKWAFGTLERYRKKFCMFND 301

Query: 321 DVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAARKTMARMLGNNE 380
           DVQGTAGVA+AGLLG VRAQGRP+ DF  QKIVV GAGSAG+GV   A + +ARM G +E
Sbjct: 302 DVQGTAGVALAGLLGTVRAQGRPISDFVNQKIVVVGAGSAGLGVTKMAVQAVARMAGISE 361

Query: 381 SAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGASLVEVVQQVKP 440
           S    A   F+++D  GL+T ER  +D  A  FA+   EI     REGAS+VEVV++V+P
Sbjct: 362 S---EATKNFYLIDKDGLVTTERTKLDPGAVLFAKNPAEI-----REGASIVEVVKKVRP 421

Query: 441 DVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPQEAFSILGENIIFAS 500
            VLLGLS VGG+F +EVL+A++ S + +PAIFAMSNPT NAECT  +AF   G NI+FAS
Sbjct: 422 HVLLGLSGVGGIFNEEVLKAMRESDSCKPAIFAMSNPTLNAECTAADAFKHAGGNIVFAS 481

Query: 501 GSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAAAECLAAYMTED 560
           GSPF++V+  NG +GH NQ NNMYLFPGIGLGTLLSG+ IV+DGMLQAA+ECLA+YMT++
Sbjct: 482 GSPFENVELENGKVGHVNQANNMYLFPGIGLGTLLSGARIVTDGMLQAASECLASYMTDE 541

Query: 561 EVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKFSKEEILEFVKN 620
           EV +GI+YPSI++IR IT E+ AAV+  A+ +D+ EG+  V  ++L   SKE+ + ++  
Sbjct: 542 EVQKGILYPSINNIRHITAEVGAAVLRAAVTDDIAEGHGDVGPKDLSHMSKEDTVNYITR 601

Query: 621 NMWSPEYPTLVYSQ 626
           NMW P Y  LV+ +
Sbjct: 602 NMWFPVYSPLVHEK 607

BLAST of CmUC05G083350 vs. ExPASy TrEMBL
Match: A0A1S3AVM9 (Malic enzyme OS=Cucumis melo OX=3656 GN=LOC103483428 PE=3 SV=1)

HSP 1 Score: 1232.2 bits (3187), Expect = 0.0e+00
Identity = 615/626 (98.24%), Postives = 625/626 (99.84%), Query Frame = 0

Query: 1   MTKWNSLSASLMKRLKLHYQMTHNIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK 60
           MTKWNSLSASLMKRLKLHYQMT++IPALAQ+RSFTTSEGHRPTIVHKRSLDILHDPWFNK
Sbjct: 1   MTKWNSLSASLMKRLKLHYQMTNSIPALAQSRSFTTSEGHRPTIVHKRSLDILHDPWFNK 60

Query: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120
           GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN
Sbjct: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120

Query: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180
           RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM
Sbjct: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180

Query: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240
           VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG
Sbjct: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240

Query: 241 TNNEKLLKDPLYLGLQEHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300
           TNNEKLLKDPLYLGLQ+HRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ
Sbjct: 241 TNNEKLLKDPLYLGLQQHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300

Query: 301 RYRNNYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360
           RYRN YRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR
Sbjct: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360

Query: 361 KTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420
           KTMARMLGNNE+AFEAARSQFWVVDAQGLITEEREN+DQDASPFARKVKEINRQGLREGA
Sbjct: 361 KTMARMLGNNEAAFEAARSQFWVVDAQGLITEERENLDQDASPFARKVKEINRQGLREGA 420

Query: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPQEAF 480
           SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTP+EAF
Sbjct: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAF 480

Query: 481 SILGENIIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540
           SILGE++IFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA
Sbjct: 481 SILGESVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540

Query: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKF 600
           AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKF
Sbjct: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKF 600

Query: 601 SKEEILEFVKNNMWSPEYPTLVYSQD 627
           SKEEILEFVKNNMWSPEYPTLVY+QD
Sbjct: 601 SKEEILEFVKNNMWSPEYPTLVYNQD 626

BLAST of CmUC05G083350 vs. ExPASy TrEMBL
Match: A0A0A0L7T0 (Malic enzyme OS=Cucumis sativus OX=3659 GN=Csa_3G127830 PE=3 SV=1)

HSP 1 Score: 1229.5 bits (3180), Expect = 0.0e+00
Identity = 614/626 (98.08%), Postives = 624/626 (99.68%), Query Frame = 0

Query: 1   MTKWNSLSASLMKRLKLHYQMTHNIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK 60
           MTKWNSLSASLMKRLKLHYQM ++IPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK
Sbjct: 1   MTKWNSLSASLMKRLKLHYQMINSIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK 60

Query: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120
           GTAFT+TERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN
Sbjct: 61  GTAFTITERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120

Query: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180
           RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSA+DRGEMMSM
Sbjct: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAQDRGEMMSM 180

Query: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240
           VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG
Sbjct: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240

Query: 241 TNNEKLLKDPLYLGLQEHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300
           TNNEKLLKDPLYLGLQ+HRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ
Sbjct: 241 TNNEKLLKDPLYLGLQQHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300

Query: 301 RYRNNYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360
           RYRN YRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR
Sbjct: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360

Query: 361 KTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420
           KTMARMLGNNE+AFEAARSQFWVVDAQGLITEER+NIDQDASPFARKVKEINRQGLREGA
Sbjct: 361 KTMARMLGNNEAAFEAARSQFWVVDAQGLITEERKNIDQDASPFARKVKEINRQGLREGA 420

Query: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPQEAF 480
           SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTP+EAF
Sbjct: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAF 480

Query: 481 SILGENIIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540
           SILGEN+IFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA
Sbjct: 481 SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540

Query: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKF 600
           AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKF
Sbjct: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKF 600

Query: 601 SKEEILEFVKNNMWSPEYPTLVYSQD 627
           SKEEILEFVKNNMWSPEYPTLVY+QD
Sbjct: 601 SKEEILEFVKNNMWSPEYPTLVYNQD 626

BLAST of CmUC05G083350 vs. ExPASy TrEMBL
Match: A0A6J1IQW3 (Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111479676 PE=3 SV=1)

HSP 1 Score: 1226.1 bits (3171), Expect = 0.0e+00
Identity = 611/626 (97.60%), Postives = 624/626 (99.68%), Query Frame = 0

Query: 1   MTKWNSLSASLMKRLKLHYQMTHNIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK 60
           MTKWNSLSASLMKRLKLHYQM+++IPALAQARSFTTSEGHRP I+HKRSLDILHDPWFNK
Sbjct: 1   MTKWNSLSASLMKRLKLHYQMSNSIPALAQARSFTTSEGHRPAIIHKRSLDILHDPWFNK 60

Query: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120
           GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN
Sbjct: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120

Query: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180
           RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM
Sbjct: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180

Query: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240
           VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG
Sbjct: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240

Query: 241 TNNEKLLKDPLYLGLQEHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300
           TNNEKLLKDPLYLGLQ++RLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ
Sbjct: 241 TNNEKLLKDPLYLGLQQNRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300

Query: 301 RYRNNYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360
           RYRN YRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR
Sbjct: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360

Query: 361 KTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420
           KTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA
Sbjct: 361 KTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420

Query: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPQEAF 480
           SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTP++AF
Sbjct: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEQAF 480

Query: 481 SILGENIIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540
           SILGEN+IFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSG+PIVSDGMLQAA
Sbjct: 481 SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGAPIVSDGMLQAA 540

Query: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKF 600
           AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVD+RELRKF
Sbjct: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDSRELRKF 600

Query: 601 SKEEILEFVKNNMWSPEYPTLVYSQD 627
           SKE+ILEFVKNNMWSPEYPTLVY+QD
Sbjct: 601 SKEQILEFVKNNMWSPEYPTLVYNQD 626

BLAST of CmUC05G083350 vs. ExPASy TrEMBL
Match: A0A6J1EA17 (Malic enzyme OS=Cucurbita moschata OX=3662 GN=LOC111432147 PE=3 SV=1)

HSP 1 Score: 1223.0 bits (3163), Expect = 0.0e+00
Identity = 609/626 (97.28%), Postives = 623/626 (99.52%), Query Frame = 0

Query: 1   MTKWNSLSASLMKRLKLHYQMTHNIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK 60
           MTKWNSLSASLMKRLKLHYQM+++IPAL+Q RSFTTSEGHRP I+HKRSLDILHDPWFNK
Sbjct: 1   MTKWNSLSASLMKRLKLHYQMSNSIPALSQVRSFTTSEGHRPAIIHKRSLDILHDPWFNK 60

Query: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120
           GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN
Sbjct: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120

Query: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180
           RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM
Sbjct: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180

Query: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240
           VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG
Sbjct: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240

Query: 241 TNNEKLLKDPLYLGLQEHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300
           TNNEKLLKDPLYLGLQ++RLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ
Sbjct: 241 TNNEKLLKDPLYLGLQQNRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300

Query: 301 RYRNNYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360
           RYRN YRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR
Sbjct: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360

Query: 361 KTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420
           KTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA
Sbjct: 361 KTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420

Query: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPQEAF 480
           SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTP++AF
Sbjct: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEQAF 480

Query: 481 SILGENIIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540
           SILGEN+IFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSG+PIVSDGMLQAA
Sbjct: 481 SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGAPIVSDGMLQAA 540

Query: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKF 600
           AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDAREL+KF
Sbjct: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELQKF 600

Query: 601 SKEEILEFVKNNMWSPEYPTLVYSQD 627
           SKE+ILEFVKNNMWSPEYPTLVY+QD
Sbjct: 601 SKEQILEFVKNNMWSPEYPTLVYNQD 626

BLAST of CmUC05G083350 vs. ExPASy TrEMBL
Match: A0A5D3D1H1 (Malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003240 PE=3 SV=1)

HSP 1 Score: 1211.1 bits (3132), Expect = 0.0e+00
Identity = 603/615 (98.05%), Postives = 614/615 (99.84%), Query Frame = 0

Query: 12  MKRLKLHYQMTHNIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNKGTAFTMTERDR 71
           MKRLKLHYQMT++IPALAQ+RSFTTSEGHRPTIVHKRSLDILHDPWFNKGTAFTMTERDR
Sbjct: 1   MKRLKLHYQMTNSIPALAQSRSFTTSEGHRPTIVHKRSLDILHDPWFNKGTAFTMTERDR 60

Query: 72  LDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILNRLHDRNETMYY 131
           LDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILNRLHDRNETMYY
Sbjct: 61  LDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILNRLHDRNETMYY 120

Query: 132 KVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPADQVDM 191
           KVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPADQVDM
Sbjct: 121 KVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPADQVDM 180

Query: 192 IVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEKLLKDPL 251
           IVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEKLLKDPL
Sbjct: 181 IVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEKLLKDPL 240

Query: 252 YLGLQEHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNNYRMFND 311
           YLGLQ+HRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRN YRMFND
Sbjct: 241 YLGLQQHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNTYRMFND 300

Query: 312 DVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAARKTMARMLGNNE 371
           DVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAARKTMARMLGNNE
Sbjct: 301 DVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAARKTMARMLGNNE 360

Query: 372 SAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGASLVEVVQQVKP 431
           +AFEAARSQFWVVDAQGLITEEREN+DQDASPFARKVKEINRQGLREGASLVEVVQQVKP
Sbjct: 361 AAFEAARSQFWVVDAQGLITEERENLDQDASPFARKVKEINRQGLREGASLVEVVQQVKP 420

Query: 432 DVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPQEAFSILGENIIFAS 491
           DVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTP+EAFSILGE++IFAS
Sbjct: 421 DVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAFSILGESVIFAS 480

Query: 492 GSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAAAECLAAYMTED 551
           GSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAAAECLAAYMTED
Sbjct: 481 GSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAAAECLAAYMTED 540

Query: 552 EVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKFSKEEILEFVKN 611
           EVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRG+DARELRKFSKEEILEFVKN
Sbjct: 541 EVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGIDARELRKFSKEEILEFVKN 600

Query: 612 NMWSPEYPTLVYSQD 627
           NMWSPEYPTLVY+QD
Sbjct: 601 NMWSPEYPTLVYNQD 615

BLAST of CmUC05G083350 vs. TAIR 10
Match: AT2G13560.1 (NAD-dependent malic enzyme 1 )

HSP 1 Score: 1063.5 bits (2749), Expect = 6.4e-311
Identity = 526/620 (84.84%), Postives = 577/620 (93.06%), Query Frame = 0

Query: 7   LSASLMKRLKLHYQMTHNIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNKGTAFTM 66
           LS+S + R+ LH ++ ++    +  RSFTTSEGHRPTIVHK+ LDILHDPWFNKGTAFTM
Sbjct: 9   LSSSSLSRI-LHRRILYS----SAVRSFTTSEGHRPTIVHKQGLDILHDPWFNKGTAFTM 68

Query: 67  TERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILNRLHDRN 126
           TER+RLDLRGLLPPNVM SEQQI RFM DLKRLE QARDGPSDPNALAKWRILNRLHDRN
Sbjct: 69  TERNRLDLRGLLPPNVMDSEQQIFRFMTDLKRLEEQARDGPSDPNALAKWRILNRLHDRN 128

Query: 127 ETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPA 186
           ETMYYKVLI +IEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPA
Sbjct: 129 ETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPA 188

Query: 187 DQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEKL 246
           +QVDMIVVTDGSRILGLGDLGVHGIGIA+GKLDLYVAAAGINPQRVLPVMIDVGTNNEKL
Sbjct: 189 EQVDMIVVTDGSRILGLGDLGVHGIGIAVGKLDLYVAAAGINPQRVLPVMIDVGTNNEKL 248

Query: 247 LKDPLYLGLQEHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNNY 306
             DP+YLGLQ+ RL+ D+Y+ +IDEFMEAV+TRWPHVIVQFEDFQSKWAFKLLQRYR  Y
Sbjct: 249 RNDPMYLGLQQRRLEDDDYIDVIDEFMEAVYTRWPHVIVQFEDFQSKWAFKLLQRYRCTY 308

Query: 307 RMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAARKTMARM 366
           RMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPK KIVVAGAGSAGIGVLNAARKTMARM
Sbjct: 309 RMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKMKIVVAGAGSAGIGVLNAARKTMARM 368

Query: 367 LGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGASLVEVV 426
           LGN E+AF++A+SQFWVVDAQGLITE RENID +A PFARK KE+ RQGL+EGA+LVEVV
Sbjct: 369 LGNTETAFDSAQSQFWVVDAQGLITEGRENIDPEAQPFARKTKEMERQGLKEGATLVEVV 428

Query: 427 QQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPQEAFSILGEN 486
           ++VKPDVLLGLSAVGGLF+KEVLEA+KGST+TRPAIFAMSNPT NAECTPQ+AFSILGEN
Sbjct: 429 REVKPDVLLGLSAVGGLFSKEVLEAMKGSTSTRPAIFAMSNPTKNAECTPQDAFSILGEN 488

Query: 487 IIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAAAECLAA 546
           +IFASGSPFK+V+FGNGH+GHCNQGNNMYLFPGIGLGTLLSG+PIVSDGMLQAA+ECLAA
Sbjct: 489 MIFASGSPFKNVEFGNGHVGHCNQGNNMYLFPGIGLGTLLSGAPIVSDGMLQAASECLAA 548

Query: 547 YMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKFSKEEIL 606
           YM+E+EV EGIIYP IS IRDITK IAAAVI EAIEEDLVEGYR +DARE++K  +E ++
Sbjct: 549 YMSEEEVLEGIIYPPISRIRDITKRIAAAVIKEAIEEDLVEGYREMDAREIQKLDEEGLM 608

Query: 607 EFVKNNMWSPEYPTLVYSQD 627
           E+V+NNMW+PEYPTLVY  D
Sbjct: 609 EYVENNMWNPEYPTLVYKDD 623

BLAST of CmUC05G083350 vs. TAIR 10
Match: AT4G00570.1 (NAD-dependent malic enzyme 2 )

HSP 1 Score: 778.5 bits (2009), Expect = 4.1e-225
Identity = 386/614 (62.87%), Postives = 478/614 (77.85%), Query Frame = 0

Query: 21  MTHNIPALAQARSFTTSEGHR---------PTIVHKRSLDILHDPWFNKGTAFTMTERDR 80
           M  NI  L++A +   + G R         P IVHKR  DILHDPWFNK T F +TERDR
Sbjct: 2   MWKNIAGLSKAAAAARTHGSRRCFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDR 61

Query: 81  LDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILNRLHDRNETMYY 140
           L +RGLLPP VM+  QQ +RF+   + LE   +  P +  ALAKWR+LNRLHDRNET+YY
Sbjct: 62  LGIRGLLPPRVMTCVQQCDRFIESFRSLENNTKGEPENVVALAKWRMLNRLHDRNETLYY 121

Query: 141 KVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPADQVDM 200
           +VLI +I+++API+YTPTVGLVCQNYSGL+RRPRGMYFSA+D+GEMMSM+YNWPA QVDM
Sbjct: 122 RVLIDNIKDFAPIIYTPTVGLVCQNYSGLYRRPRGMYFSAKDKGEMMSMIYNWPAPQVDM 181

Query: 201 IVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEKLLKDPL 260
           IV+TDGSRILGLGDLGV GIGI IGKLD+YVAAAGINPQRVLP+M+DVGTNNEKLL++ L
Sbjct: 182 IVITDGSRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRVLPIMLDVGTNNEKLLQNDL 241

Query: 261 YLGLQEHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNNYRMFND 320
           YLG+++ RL+G+EYL IIDEFMEA FTRWP  +VQFEDFQ+KWAF  L+RYR  + MFND
Sbjct: 242 YLGVRQPRLEGEEYLEIIDEFMEAAFTRWPKAVVQFEDFQAKWAFGTLERYRKKFCMFND 301

Query: 321 DVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAARKTMARMLGNNE 380
           DVQGTAGVA+AGLLG VRAQGRP+ DF  QKIVV GAGSAG+GV   A + +ARM G +E
Sbjct: 302 DVQGTAGVALAGLLGTVRAQGRPISDFVNQKIVVVGAGSAGLGVTKMAVQAVARMAGISE 361

Query: 381 SAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGASLVEVVQQVKP 440
           S    A   F+++D  GL+T ER  +D  A  FA+   EI     REGAS+VEVV++V+P
Sbjct: 362 S---EATKNFYLIDKDGLVTTERTKLDPGAVLFAKNPAEI-----REGASIVEVVKKVRP 421

Query: 441 DVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPQEAFSILGENIIFAS 500
            VLLGLS VGG+F +EVL+A++ S + +PAIFAMSNPT NAECT  +AF   G NI+FAS
Sbjct: 422 HVLLGLSGVGGIFNEEVLKAMRESDSCKPAIFAMSNPTLNAECTAADAFKHAGGNIVFAS 481

Query: 501 GSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAAAECLAAYMTED 560
           GSPF++V+  NG +GH NQ NNMYLFPGIGLGTLLSG+ IV+DGMLQAA+ECLA+YMT++
Sbjct: 482 GSPFENVELENGKVGHVNQANNMYLFPGIGLGTLLSGARIVTDGMLQAASECLASYMTDE 541

Query: 561 EVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKFSKEEILEFVKN 620
           EV +GI+YPSI++IR IT E+ AAV+  A+ +D+ EG+  V  ++L   SKE+ + ++  
Sbjct: 542 EVQKGILYPSINNIRHITAEVGAAVLRAAVTDDIAEGHGDVGPKDLSHMSKEDTVNYITR 601

Query: 621 NMWSPEYPTLVYSQ 626
           NMW P Y  LV+ +
Sbjct: 602 NMWFPVYSPLVHEK 607

BLAST of CmUC05G083350 vs. TAIR 10
Match: AT2G19900.1 (NADP-malic enzyme 1 )

HSP 1 Score: 432.6 bits (1111), Expect = 5.5e-121
Identity = 241/569 (42.36%), Postives = 351/569 (61.69%), Query Frame = 0

Query: 52  ILHDPWFNKGTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPN 111
           +L DP +NKG AFT  ERD   LRGLLPP V+  + Q +R + ++++ +           
Sbjct: 45  LLRDPRYNKGLAFTEKERDTHYLRGLLPPVVLDQKLQEKRLLNNIRQYQF---------- 104

Query: 112 ALAKWRILNRLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSA 171
            L K+  L  L +RNE ++YK+LI ++EE  PIVYTPTVG  CQ +  +FRRP+G++ S 
Sbjct: 105 PLQKYMALTELQERNERLFYKLLIDNVEELLPIVYTPTVGEACQKFGSIFRRPQGLFISL 164

Query: 172 EDRGEMMSMVYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQR 231
           +D+G+++ ++ NWP   + +IVVTDG RILGLGDLG  G+GI +GKL LY A  G+ P  
Sbjct: 165 KDKGKILDVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYSALGGVRPSA 224

Query: 232 VLPVMIDVGTNNEKLLKDPLYLGLQEHRLDGDEYLAIIDEFMEAVFTRW-PHVIVQFEDF 291
            LPV IDVGTNNEKLL D  Y+GL++ R  G EY  +++EFM AV   +   V++QFEDF
Sbjct: 225 CLPVTIDVGTNNEKLLNDEFYIGLRQKRATGQEYSELLNEFMSAVKQNYGEKVLIQFEDF 284

Query: 292 QSKWAFKLLQRYRNNYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGS 351
            +  AF+LL +Y + + +FNDD+QGTA V +AGL+ A +    P+ +      +  GAG 
Sbjct: 285 ANHNAFELLAKYSDTHLVFNDDIQGTASVVLAGLVSAQKLTNSPLAE---HTFLFLGAGE 344

Query: 352 AGIGVLNAARKTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQD-ASPFARKVK 411
           AG G+       M++ +    ++ E +R + W+VD++GLI   R++  QD   P+A + +
Sbjct: 345 AGTGIAELIALYMSKQM---NASVEESRKKIWLVDSKGLIVNSRKDSLQDFKKPWAHEHE 404

Query: 412 EINRQGLREGASLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPT 471
            +          L+  ++ +KP VL+G S VG  FTKEV+EA+  S   RP I A+SNPT
Sbjct: 405 PVK--------DLLGAIKAIKPTVLIGSSGVGRSFTKEVIEAM-SSINERPLIMALSNPT 464

Query: 472 TNAECTPQEAFSILGENIIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGS 531
           T +ECT +EA++      IFASGSPF  V++  G +    Q NN Y+FPG GLG ++SG+
Sbjct: 465 TQSECTAEEAYTWSKGRAIFASGSPFDPVEY-EGKVFVSTQANNAYIFPGFGLGLVISGA 524

Query: 532 PIVSDGMLQAAAECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGY 591
             V D ML AAAE LA  ++++   +G+IYPS SSIR I+ +IAA V  +A E  L    
Sbjct: 525 IRVHDDMLLAAAEALAGQVSKENYEKGMIYPSFSSIRKISAQIAANVATKAYELGLAG-- 577

Query: 592 RGVDARELRKFSKEEILEFVKNNMWSPEY 619
                   R    ++I++  +++M+SP Y
Sbjct: 585 --------RLPRPKDIVKCAESSMYSPTY 577

BLAST of CmUC05G083350 vs. TAIR 10
Match: AT5G11670.1 (NADP-malic enzyme 2 )

HSP 1 Score: 420.2 bits (1079), Expect = 2.8e-117
Identity = 234/569 (41.12%), Postives = 340/569 (59.75%), Query Frame = 0

Query: 52  ILHDPWFNKGTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPN 111
           ++ DP +NKG AFT  ERD   L GLLPP ++S + Q  + M +L++  V          
Sbjct: 52  LMRDPRYNKGLAFTDKERDAHYLTGLLPPVILSQDVQERKVMHNLRQYTV---------- 111

Query: 112 ALAKWRILNRLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSA 171
            L ++  L  L +RNE ++YK+LI ++EE  P+VYTPTVG  CQ Y  +FR+P+G+Y S 
Sbjct: 112 PLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRKPQGLYISL 171

Query: 172 EDRGEMMSMVYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQR 231
            ++G+++ ++ NWP   + +IVVTDG RILGLGDLG  G+GI +GKL LY A  GI P  
Sbjct: 172 NEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSA 231

Query: 232 VLPVMIDVGTNNEKLLKDPLYLGLQEHRLDGDEYLAIIDEFMEAVFTRW-PHVIVQFEDF 291
            LP+ IDVGTNNEKLL D  Y+GL++ R  G EY   + EFM AV   +   V+VQFEDF
Sbjct: 232 CLPITIDVGTNNEKLLNDEFYIGLKQRRATGQEYAEFLHEFMCAVKQNYGEKVLVQFEDF 291

Query: 292 QSKWAFKLLQRYRNNYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGS 351
            +  AF LL +Y +++ +FNDD+QGTA V +AGL+ A +  G+ + D      +  GAG 
Sbjct: 292 ANHNAFDLLSKYSDSHLVFNDDIQGTASVVLAGLIAAQKVLGKKLAD---HTFLFLGAGE 351

Query: 352 AGIGVLNAARKTMARMLGNNESAFEAARSQFWVVDAQGLITEER-ENIDQDASPFARKVK 411
           AG G+       +++  G   +     R + W+VD++GLI   R E++     P+A + K
Sbjct: 352 AGTGIAELIALKISKETG---APITETRKKIWLVDSKGLIVSSRKESLQHFKQPWAHEHK 411

Query: 412 EINRQGLREGASLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPT 471
            +          L+  V  +KP VL+G S VG  FTKEV+EA+  +   +P I A+SNPT
Sbjct: 412 PVK--------DLIGAVNAIKPTVLIGTSGVGQTFTKEVVEAM-ATNNEKPLILALSNPT 471

Query: 472 TNAECTPQEAFSILGENIIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGS 531
           + AECT ++A++      IF SGSPF  V + +G      Q NN Y+FPG+GLG ++SG+
Sbjct: 472 SQAECTAEQAYTWTKGRAIFGSGSPFDPVVY-DGKTYLPGQANNCYIFPGLGLGLIMSGA 531

Query: 532 PIVSDGMLQAAAECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGY 591
             V D ML AA+E LAA +TE+    G+IYP  S+IR+I+  IAA V  +  +  L    
Sbjct: 532 IRVRDDMLLAASEALAAQVTEEHYANGLIYPPFSNIREISANIAACVAAKTYDLGLASNL 584

Query: 592 RGVDARELRKFSKEEILEFVKNNMWSPEY 619
                        +++++F +++M+SP Y
Sbjct: 592 P----------RAKDLVKFAESSMYSPVY 584

BLAST of CmUC05G083350 vs. TAIR 10
Match: AT5G25880.1 (NADP-malic enzyme 3 )

HSP 1 Score: 418.3 bits (1074), Expect = 1.1e-116
Identity = 234/569 (41.12%), Postives = 343/569 (60.28%), Query Frame = 0

Query: 52  ILHDPWFNKGTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPN 111
           ++ DP +NKG AFT  ERD   + GLLPP V+S + Q  + M +L++  V          
Sbjct: 52  LMRDPRYNKGLAFTDKERDAHYITGLLPPVVLSQDVQERKVMHNLRQYTV---------- 111

Query: 112 ALAKWRILNRLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSA 171
            L ++  L  L +RNE ++YK+LI ++EE  P+VYTPTVG  CQ Y  ++RRP+G+Y S 
Sbjct: 112 PLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLYISL 171

Query: 172 EDRGEMMSMVYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQR 231
           +++G+++ ++ NWP   + +IVVTDG RILGLGDLG  G+GI +GKL LY A  GI P  
Sbjct: 172 KEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSA 231

Query: 232 VLPVMIDVGTNNEKLLKDPLYLGLQEHRLDGDEYLAIIDEFMEAVFTRW-PHVIVQFEDF 291
            LP+ IDVGTNNEKLL +  Y+GL++ R +G+EY   + EFM AV   +   V+VQFEDF
Sbjct: 232 CLPITIDVGTNNEKLLNNEFYIGLKQKRANGEEYAEFLQEFMCAVKQNYGEKVLVQFEDF 291

Query: 292 QSKWAFKLLQRYRNNYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGS 351
            +  AF+LL +Y +++ +FNDD+QGTA V +AGL+ A +  G+ + D      +  GAG 
Sbjct: 292 ANHHAFELLSKYCSSHLVFNDDIQGTASVVLAGLIAAQKVLGKSLAD---HTFLFLGAGE 351

Query: 352 AGIGVLNAARKTMARMLGNNESAFEAARSQFWVVDAQGLITEER-ENIDQDASPFARKVK 411
           AG G+       +++  G      +  R + W+VD++GLI  ER E++     P+A   K
Sbjct: 352 AGTGIAELIALKISKETG---KPIDETRKKIWLVDSKGLIVSERKESLQHFKQPWAHDHK 411

Query: 412 EINRQGLREGASLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPT 471
            +          L+  V  +KP VL+G S VG  FTKEV+EA+  +   +P I A+SNPT
Sbjct: 412 PVK--------ELLAAVNAIKPTVLIGTSGVGKTFTKEVVEAM-ATLNEKPLILALSNPT 471

Query: 472 TNAECTPQEAFSILGENIIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGS 531
           + AECT +EA++      IFASGSPF  V + +G      Q NN Y+FPG+GLG ++SG+
Sbjct: 472 SQAECTAEEAYTWTKGRAIFASGSPFDPVQY-DGKKFTPGQANNCYIFPGLGLGLIMSGA 531

Query: 532 PIVSDGMLQAAAECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGY 591
             V D ML AA+E LA+ +TE+    G+IYP  ++IR I+  IAA+V  +  E  L    
Sbjct: 532 IRVRDDMLLAASEALASQVTEENFANGLIYPPFANIRKISANIAASVGAKTYELGLASNL 584

Query: 592 RGVDARELRKFSKEEILEFVKNNMWSPEY 619
                        +++++  ++ M+SP Y
Sbjct: 592 P----------RPKDLVKMAESCMYSPVY 584

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008438266.10.0e+0098.24PREDICTED: NAD-dependent malic enzyme 62 kDa isoform, mitochondrial isoform X1 [... [more]
XP_004133957.10.0e+0098.08NAD-dependent malic enzyme 1, mitochondrial isoform X1 [Cucumis sativus] >KGN566... [more]
XP_023528517.10.0e+0097.76NAD-dependent malic enzyme 1, mitochondrial isoform X1 [Cucurbita pepo subsp. pe... [more]
XP_022980252.10.0e+0097.60NAD-dependent malic enzyme 1, mitochondrial isoform X1 [Cucurbita maxima][more]
KAG7018901.10.0e+0097.60NAD-dependent malic enzyme 1, mitochondrial [Cucurbita argyrosperma subsp. argyr... [more]
Match NameE-valueIdentityDescription
Q9SIU09.0e-31084.84NAD-dependent malic enzyme 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=N... [more]
P372212.0e-30984.84NAD-dependent malic enzyme 62 kDa isoform, mitochondrial OS=Solanum tuberosum OX... [more]
P372248.5e-28478.35NAD-dependent malic enzyme 65 kDa isoform, mitochondrial OS=Amaranthus hypochond... [more]
P372252.7e-22967.01NAD-dependent malic enzyme 59 kDa isoform, mitochondrial OS=Solanum tuberosum OX... [more]
Q8L7K95.8e-22462.87NAD-dependent malic enzyme 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=N... [more]
Match NameE-valueIdentityDescription
A0A1S3AVM90.0e+0098.24Malic enzyme OS=Cucumis melo OX=3656 GN=LOC103483428 PE=3 SV=1[more]
A0A0A0L7T00.0e+0098.08Malic enzyme OS=Cucumis sativus OX=3659 GN=Csa_3G127830 PE=3 SV=1[more]
A0A6J1IQW30.0e+0097.60Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111479676 PE=3 SV=1[more]
A0A6J1EA170.0e+0097.28Malic enzyme OS=Cucurbita moschata OX=3662 GN=LOC111432147 PE=3 SV=1[more]
A0A5D3D1H10.0e+0098.05Malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003240 ... [more]
Match NameE-valueIdentityDescription
AT2G13560.16.4e-31184.84NAD-dependent malic enzyme 1 [more]
AT4G00570.14.1e-22562.87NAD-dependent malic enzyme 2 [more]
AT2G19900.15.5e-12142.36NADP-malic enzyme 1 [more]
AT5G11670.12.8e-11741.12NADP-malic enzyme 2 [more]
AT5G25880.11.1e-11641.12NADP-malic enzyme 3 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 82..102
NoneNo IPR availableGENE3D3.40.50.720coord: 311..626
e-value: 2.4E-99
score: 334.5
NoneNo IPR availablePIRSRPIRSR000106-1PIRSR000106-1coord: 52..394
e-value: 8.0E-121
score: 402.1
NoneNo IPR availablePIRSRPIRSR000106-3PIRSR000106-3coord: 51..399
e-value: 5.5E-131
score: 435.5
NoneNo IPR availablePANTHERPTHR23406MALIC ENZYME-RELATEDcoord: 36..622
NoneNo IPR availablePANTHERPTHR23406:SF78MALIC ENZYMEcoord: 36..622
NoneNo IPR availableCDDcd05312NAD_bind_1_malic_enzcoord: 313..616
e-value: 1.5776E-121
score: 359.555
NoneNo IPR availableSUPERFAMILY53223Aminoacid dehydrogenase-like, N-terminal domaincoord: 20..312
IPR001891Malic oxidoreductasePRINTSPR00072MALOXRDTASEcoord: 225..247
score: 64.02
coord: 309..325
score: 70.74
coord: 343..359
score: 43.82
coord: 284..302
score: 53.95
coord: 457..473
score: 56.76
coord: 189..218
score: 70.25
coord: 129..153
score: 54.1
IPR001891Malic oxidoreductasePIRSFPIRSF000106MEcoord: 16..624
e-value: 1.8E-220
score: 731.3
IPR012302Malic enzyme, NAD-bindingSMARTSM00919Malic_M_2coord: 313..581
e-value: 1.0E-85
score: 300.8
IPR012302Malic enzyme, NAD-bindingPFAMPF03949Malic_Mcoord: 313..580
e-value: 4.0E-91
score: 304.8
IPR012301Malic enzyme, N-terminal domainSMARTSM01274malic_2coord: 123..303
e-value: 1.8E-97
score: 339.8
IPR012301Malic enzyme, N-terminal domainPFAMPF00390maliccoord: 123..303
e-value: 8.5E-77
score: 257.1
IPR037062Malic enzyme, N-terminal domain superfamilyGENE3D3.40.50.10380coord: 47..310
e-value: 1.0E-123
score: 413.5
IPR015884Malic enzyme, conserved sitePROSITEPS00331MALIC_ENZYMEScoord: 309..325
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 313..622

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmUC05G083350.1CmUC05G083350.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006108 malate metabolic process
biological_process GO:0006090 pyruvate metabolic process
molecular_function GO:0004471 malate dehydrogenase (decarboxylating) (NAD+) activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0051287 NAD binding
molecular_function GO:0042803 protein homodimerization activity
molecular_function GO:0004470 malic enzyme activity