CmUC04G079450 (gene) Watermelon (USVL531) v1

Overview


Name	CmUC04G079450
Type	gene
Organism	Citrullus mucosospermus (Watermelon (USVL531) v1)
Description	Calcium-binding EF-hand family protein
Location	CmU531Chr04: 25786781 .. 25787482 (-)
RNA-Seq Expression	CmUC04G079450
Synteny	CmUC04G079450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAATTCTTCTATTGTCTGCTGCTTACTACTCAATTATAGATATATCTACTATTTCTTCTTTCACTCTTTCGAAAATGGCACCCATTGATGAGCTTGATAATGCTAGCCCCATTTCAGTTTTCCAGATTTTGTTTCTCATTTTTCTCAATTTCGTACTTGAACGTTTCTTTTCAGGTTTTTTATACATTTTCATACTCTGTGCCGCGTTCCTTTTTTATCTGTGGAATTCATGGGCTGCCCACCGCAACATCATCAAACGCCTTGAGGCGGTTGATGCGACATGCACACCAAGTGTTCGACCAAATGCCCATGTGGGACATTGCTACACAGAAATTGTGGGCATGCCCGTTAATGACACACAGGAACTGAAACTATCGATTGAAGATGTGAAAACTATGATGGAGGCACTTGATCATGAGTATAACAACAGGGAAAGTAATAATGCTGAGATTGAGGGGCTATTTGAGGAAGAATTGAGTTTGGGGGAAGTTAAGGAGGCTTTTGATTTGTTTGATGAGAATGGTGATGGGTTTATTGATGCAGAGGACTTGAAGAAAGTGTTGTGTGGCATGGGATTTGCAGTGGCTTCAGAAGTTGATCAATGCAGGAGAATGATAAGTGGGTTTGACAATAATGGAGATGGGCGTTTAGATTTTGAAGACTTTGCTAAGCTTGTTGAACAGAGCTTTTGTGGATAA

mRNA sequence

ATGGGAATTCTTCTATTGTCTGCTGCTTACTACTCAATTATAGATATATCTACTATTTCTTCTTTCACTCTTTCGAAAATGGCACCCATTGATGAGCTTGATAATGCTAGCCCCATTTCAGTTTTCCAGATTTTGTTTCTCATTTTTCTCAATTTCGTACTTGAACGTTTCTTTTCAGGTTTTTTATACATTTTCATACTCTGTGCCGCGTTCCTTTTTTATCTGTGGAATTCATGGGCTGCCCACCGCAACATCATCAAACGCCTTGAGGCGGTTGATGCGACATGCACACCAAGTGTTCGACCAAATGCCCATGTGGGACATTGCTACACAGAAATTGTGGGCATGCCCGTTAATGACACACAGGAACTGAAACTATCGATTGAAGATGTGAAAACTATGATGGAGGCACTTGATCATGAGTATAACAACAGGGAAAGTAATAATGCTGAGATTGAGGGGCTATTTGAGGAAGAATTGAGTTTGGGGGAAGTTAAGGAGGCTTTTGATTTGTTTGATGAGAATGGTGATGGGTTTATTGATGCAGAGGACTTGAAGAAAGTGTTGTGTGGCATGGGATTTGCAGTGGCTTCAGAAGTTGATCAATGCAGGAGAATGATAAGTGGGTTTGACAATAATGGAGATGGGCGTTTAGATTTTGAAGACTTTGCTAAGCTTGTTGAACAGAGCTTTTGTGGATAA

Coding sequence (CDS)

ATGGGAATTCTTCTATTGTCTGCTGCTTACTACTCAATTATAGATATATCTACTATTTCTTCTTTCACTCTTTCGAAAATGGCACCCATTGATGAGCTTGATAATGCTAGCCCCATTTCAGTTTTCCAGATTTTGTTTCTCATTTTTCTCAATTTCGTACTTGAACGTTTCTTTTCAGGTTTTTTATACATTTTCATACTCTGTGCCGCGTTCCTTTTTTATCTGTGGAATTCATGGGCTGCCCACCGCAACATCATCAAACGCCTTGAGGCGGTTGATGCGACATGCACACCAAGTGTTCGACCAAATGCCCATGTGGGACATTGCTACACAGAAATTGTGGGCATGCCCGTTAATGACACACAGGAACTGAAACTATCGATTGAAGATGTGAAAACTATGATGGAGGCACTTGATCATGAGTATAACAACAGGGAAAGTAATAATGCTGAGATTGAGGGGCTATTTGAGGAAGAATTGAGTTTGGGGGAAGTTAAGGAGGCTTTTGATTTGTTTGATGAGAATGGTGATGGGTTTATTGATGCAGAGGACTTGAAGAAAGTGTTGTGTGGCATGGGATTTGCAGTGGCTTCAGAAGTTGATCAATGCAGGAGAATGATAAGTGGGTTTGACAATAATGGAGATGGGCGTTTAGATTTTGAAGACTTTGCTAAGCTTGTTGAACAGAGCTTTTGTGGATAA

Protein sequence

MGILLLSAAYYSIIDISTISSFTLSKMAPIDELDNASPISVFQILFLIFLNFVLERFFSGFLYIFILCAAFLFYLWNSWAAHRNIIKRLEAVDATCTPSVRPNAHVGHCYTEIVGMPVNDTQELKLSIEDVKTMMEALDHEYNNRESNNAEIEGLFEEELSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGMGFAVASEVDQCRRMISGFDNNGDGRLDFEDFAKLVEQSFCG

Homology

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BLAST of CmUC04G079450 vs. NCBI nr
Match: XP_038895011.1 (probable calcium-binding protein CML45 [Benincasa hispida])

HSP 1 Score: 357.1 bits (915), Expect = 1.2e-94
Identity = 182/209 (87.08%), Postives = 192/209 (91.87%), Query Frame = 0


Query: 27  MAPIDELDNASPISVFQILFLIFLNFVLERFFSGFLYIFILCAAFLFYLWNSWAAHRNII 86
           MAPIDELD ASPISVFQILFLIFLNFVLERFF G LY+FILCAAFLFYL NSWAAHRNII
Sbjct: 1   MAPIDELDYASPISVFQILFLIFLNFVLERFFPGLLYVFILCAAFLFYLCNSWAAHRNII 60

Query: 87  KRLEAVDATCTPSVRPNAHVGHCYTEIVGMPVNDTQELKLSIEDVKTMMEALDHEYNNRE 146
           KRLEA DATCTPSVR NAH GHCYT+IV MPVNDT+E+KL+IEDVKTMME LDHE+NN E
Sbjct: 61  KRLEAADATCTPSVRRNAHEGHCYTDIVVMPVNDTKEVKLTIEDVKTMMETLDHEHNNAE 120

Query: 147 SN----NAEIEGLFEEELSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGMGFAVASEVDQ 206
           SN    NAEI GLFEEELSLGEVKEAFDLFD+NGDGFID EDLKKVLCGMGFAVA EVDQ
Sbjct: 121 SNNLELNAEIVGLFEEELSLGEVKEAFDLFDDNGDGFIDVEDLKKVLCGMGFAVALEVDQ 180

Query: 207 CRRMISGFDNNGDGRLDFEDFAKLVEQSF 232
           CRRMISGFD+NGDGR+DFE+FAKLVEQSF
Sbjct: 181 CRRMISGFDSNGDGRIDFEEFAKLVEQSF 209

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BLAST of CmUC04G079450 vs. NCBI nr
Match: XP_023519469.1 (probable calcium-binding protein CML47 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 332.8 bits (852), Expect = 2.4e-87
Identity = 173/219 (79.00%), Postives = 189/219 (86.30%), Query Frame = 0


Query: 19  ISSFTLSKMAPIDELDNASPISVFQILFLIFLNFVLERFFSGFLYIFILCAAFLFYLWNS 78
           +  FTLS+MAPIDELD A+PISVFQILFL FLNF+LE  F G LY+FILCAAF FYLWNS
Sbjct: 4   VHPFTLSQMAPIDELDYANPISVFQILFLFFLNFLLEYLFPGPLYVFILCAAFHFYLWNS 63

Query: 79  WAAHRNIIKRLEAV-DATCTPSVRPNAHVGHCYTEIVGMPVNDTQELKLSIEDVKTMMEA 138
           W AHRNIIKR EA  DATCTPS R NAHVGHCYT IVGMPVND  E+KL+I+DVKTMMEA
Sbjct: 64  WVAHRNIIKRFEAADDATCTPSARRNAHVGHCYTGIVGMPVNDAYEVKLTIDDVKTMMEA 123

Query: 139 LDHEYNNRESNN----AEIEGLFEEELSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGMG 198
           L   +NN E++N    AEIEG+FEEELS+GEVKEAFDLFDENGDGFIDAEDLKKVL  MG
Sbjct: 124 L---HNNMETSNLELKAEIEGVFEEELSVGEVKEAFDLFDENGDGFIDAEDLKKVLDAMG 183

Query: 199 FAVASEVDQCRRMISGFDNNGDGRLDFEDFAKLVEQSFC 233
           F VASEVD+CRRMISG+D NGDGRLDFE+FAKLVEQSFC
Sbjct: 184 FTVASEVDECRRMISGYDKNGDGRLDFEEFAKLVEQSFC 219

[Close]

BLAST of CmUC04G079450 vs. NCBI nr
Match: XP_023001351.1 (probable calcium-binding protein CML47 [Cucurbita maxima])

HSP 1 Score: 332.4 bits (851), Expect = 3.1e-87
Identity = 172/219 (78.54%), Postives = 189/219 (86.30%), Query Frame = 0


Query: 19  ISSFTLSKMAPIDELDNASPISVFQILFLIFLNFVLERFFSGFLYIFILCAAFLFYLWNS 78
           +  FTLS+MAPIDELD A+PISVFQILFL FLNF+LE  F G LY+FILCAAF FYLWNS
Sbjct: 4   VHPFTLSQMAPIDELDYANPISVFQILFLFFLNFLLEYLFPGPLYVFILCAAFHFYLWNS 63

Query: 79  WAAHRNIIKRLEAV-DATCTPSVRPNAHVGHCYTEIVGMPVNDTQELKLSIEDVKTMMEA 138
           W AHRNIIKR EA  DATCTPS R NAH+GHCYT IVGMPVND  E+KL+IEDVKTMM+A
Sbjct: 64  WVAHRNIIKRFEAADDATCTPSARRNAHMGHCYTGIVGMPVNDAHEVKLTIEDVKTMMDA 123

Query: 139 LDHEYNNRESNN----AEIEGLFEEELSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGMG 198
           L   +NN E+NN    AEIEG+FEEELS+GEV+EAFDLFDENGDGFIDAEDLKKVL  MG
Sbjct: 124 L---HNNMETNNLELKAEIEGVFEEELSVGEVEEAFDLFDENGDGFIDAEDLKKVLDAMG 183

Query: 199 FAVASEVDQCRRMISGFDNNGDGRLDFEDFAKLVEQSFC 233
           F VASEVD+CRRMISG+D NGDGRLDFE+FAKLVEQSFC
Sbjct: 184 FTVASEVDECRRMISGYDKNGDGRLDFEEFAKLVEQSFC 219

[Close]

BLAST of CmUC04G079450 vs. NCBI nr
Match: KAG7020054.1 (putative calcium-binding protein CML45, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 327.8 bits (839), Expect = 7.7e-86
Identity = 170/211 (80.57%), Postives = 183/211 (86.73%), Query Frame = 0


Query: 27  MAPIDELDNASPISVFQILFLIFLNFVLERFFSGFLYIFILCAAFLFYLWNSWAAHRNII 86
           MAPIDELD A+PISVFQILFL FLNF+LE  F G LY+F+LCAAF FYLWNSW AHRNII
Sbjct: 1   MAPIDELDYANPISVFQILFLFFLNFLLEYLFPGPLYVFMLCAAFHFYLWNSWVAHRNII 60

Query: 87  KRLEAVD-ATCTPSVRPNAHVGHCYTEIVGMPVNDTQELKLSIEDVKTMMEALDHEYNNR 146
           KR EA D ATCTPS R NAHVGHCYT IVGMPVND  E+KL+IEDVKTMMEAL   +NN 
Sbjct: 61  KRFEAADGATCTPSARRNAHVGHCYTGIVGMPVNDAHEVKLTIEDVKTMMEAL---HNNM 120

Query: 147 ESNN----AEIEGLFEEELSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGMGFAVASEVD 206
           E+NN    AEIEG+FEEELS+GEVKEAFDLFDENGDGFIDAEDLKKVL  MGF VASEVD
Sbjct: 121 ETNNLELKAEIEGVFEEELSVGEVKEAFDLFDENGDGFIDAEDLKKVLDAMGFTVASEVD 180

Query: 207 QCRRMISGFDNNGDGRLDFEDFAKLVEQSFC 233
           +CRRMISG+D NGDGRLDFE+FAKLVEQSFC
Sbjct: 181 ECRRMISGYDKNGDGRLDFEEFAKLVEQSFC 208

[Close]

BLAST of CmUC04G079450 vs. NCBI nr
Match: KAA0036089.1 (putative calcium-binding protein CML30 [Cucumis melo var. makuwa] >TYJ98897.1 putative calcium-binding protein CML30 [Cucumis melo var. makuwa])

HSP 1 Score: 322.0 bits (824), Expect = 4.2e-84
Identity = 163/211 (77.25%), Postives = 182/211 (86.26%), Query Frame = 0


Query: 27  MAPIDELDNASPISVFQILFLIFLNFVLERFFSGFLYIFILCAAFLFYLWNSWAAHRNII 86
           MAP D +D  +PISVF IL L FLNF+LERFF G  YIFIL AAFLFYLWNSWAA RNII
Sbjct: 1   MAPTDVIDYTNPISVFHILCLFFLNFILERFFPGLFYIFILYAAFLFYLWNSWAAQRNII 60

Query: 87  KRLEAVDATCTPSVRPNAHVGHCYTEIVGMPVNDTQELKLSIEDVKTMMEALDHEY-NNR 146
           +RLEA D TCTPSVR N H  HC T+IVG PVND +E+KLSIEDVKTMME LDHE+ NNR
Sbjct: 61  RRLEAADVTCTPSVRQNDHEEHCCTKIVGTPVNDKREVKLSIEDVKTMMETLDHEHNNNR 120

Query: 147 ESNN----AEIEGLFEEELSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGMGFAVASEVD 206
           +S+N    AE+E +FEEELSLGEV+EAFDLFDENGDGFIDAEDL+KVLCG+GFAVAS++D
Sbjct: 121 DSDNLGLSAELEEMFEEELSLGEVQEAFDLFDENGDGFIDAEDLEKVLCGLGFAVASDID 180

Query: 207 QCRRMISGFDNNGDGRLDFEDFAKLVEQSFC 233
           QC+ MISGFDNNGDGRLDFE+FAKLVEQSFC
Sbjct: 181 QCKTMISGFDNNGDGRLDFEEFAKLVEQSFC 211

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BLAST of CmUC04G079450 vs. ExPASy Swiss-Prot
Match: Q93Z27 (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 2.9e-19
Identity = 56/129 (43.41%), Postives = 78/129 (60.47%), Query Frame = 0


Query: 111 TEIVGMPVNDTQELKLSIEDVKTMMEAL------DHEYNNRESNNAEIEGLFEE-ELSLG 170
           T I      D  +  L  EDV  +M++L      ++E   ++ ++ E+  LFEE E SL 
Sbjct: 76  TSIKHEEYRDDDDDGLCREDVGMVMKSLGLSTDQENEGLQKQYSSKEVSNLFEEKEPSLE 135

Query: 171 EVKEAFDLFDENGDGFIDAEDLKKVLCGMGFAVASEVDQCRRMISGFDNNGDGRLDFEDF 230
           EVK+AFD+FDEN DGFID  DL++VL  +G    S ++ CRRMI  FD + DGR+DF  F
Sbjct: 136 EVKQAFDVFDENRDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDFYGF 195

Query: 231 AKLVEQSFC 233
            K +E +FC
Sbjct: 196 VKFMENNFC 204

[Close]

BLAST of CmUC04G079450 vs. ExPASy Swiss-Prot
Match: Q9MBG5 (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.1e-18
Identity = 52/118 (44.07%), Postives = 75/118 (63.56%), Query Frame = 0


Query: 119 NDTQELKLSIEDVKTMMEALDHEYNN----RESNNAEIEGLFEE-ELSLGEVKEAFDLFD 178
           +D  ++ +S E+ + +M +L   YN+     + +  E+  LFEE E SL EVK+AFD+FD
Sbjct: 76  DDDDDIDISREEAEMVMRSLGLFYNDDQLQEQYSAKEVSSLFEEKEASLEEVKQAFDVFD 135

Query: 179 ENGDGFIDAEDLKKVLCGMGFAVASEVDQCRRMISGFDNNGDGRLDFEDFAKLVEQSF 232
           EN DGFIDA +L++VL  +GF   S +D C  MI   D N DG++DF +F K +E SF
Sbjct: 136 ENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMETSF 193

[Close]

BLAST of CmUC04G079450 vs. ExPASy Swiss-Prot
Match: Q9SN89 (Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 PE=2 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 5.3e-13
Identity = 63/200 (31.50%), Postives = 105/200 (52.50%), Query Frame = 0


Query: 37  SPISVFQI---LFLIFLNFVLERFFSGFLYIFILCAAFLFYLWNSWAAHRNIIKRLEAVD 96
           SPIS+  I   LF++ L  +++ F S F + F L  +  + L+ S   ++          
Sbjct: 8   SPISLLTIVIFLFILNLMMIIQDFSSSFPFRFHLFFSNAYILFTSIRNNK---------Q 67

Query: 97  ATCTPSVRPNAHVGHCYTEIVGMPVNDTQELKLSIEDVKTMMEALDHEYNNRESNNAEIE 156
            T  P ++              + V +  ++K S+E+VK +++  +  Y        E  
Sbjct: 68  NTELPIIKK-------------VVVPNRADIKTSVEEVKAIIDDSEALYECLIEEGEEY- 127

Query: 157 GLFEEELSLGE--VKEAFDLFDENGDGFIDAEDLKKVLCGMGFAVASEVDQCRRMISGFD 216
            L E+   +G+  VKEAF LFDEN DGFID  +LK VL  +G+   +++ +CR+M+  +D
Sbjct: 128 -LLEKNEMMGKEIVKEAFRLFDENQDGFIDENELKHVLSLLGYDECTKM-ECRKMVKVYD 182

Query: 217 NNGDGRLDFEDFAKLVEQSF 232
            N DG++DF +F KL+E+SF
Sbjct: 188 ENRDGKIDFYEFVKLIEKSF 182

[Close]

BLAST of CmUC04G079450 vs. ExPASy Swiss-Prot
Match: Q2QVI1 (Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 GN=CML28 PE=2 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 9.9e-12
Identity = 30/63 (47.62%), Postives = 46/63 (73.02%), Query Frame = 0


Query: 165 VKEAFDLFDENGDGFIDAEDLKKVLCGMGFAVASEVDQCRRMISGFDNNGDGRLDFEDFA 224
           ++EAF++FD+NGDGFI  ++L+ VL  +G       D CRRMIS  D +GDGR+DF++F 
Sbjct: 100 MREAFNVFDQNGDGFITVDELRSVLSSLGLKHGRTADDCRRMISMVDADGDGRVDFKEFK 159

Query: 225 KLV 228
           +++
Sbjct: 160 QMM 162

[Close]

BLAST of CmUC04G079450 vs. ExPASy Swiss-Prot
Match: Q09011 (Calcium-binding protein CAST OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.3e-11
Identity = 42/112 (37.50%), Postives = 64/112 (57.14%), Query Frame = 0


Query: 124 LKLSIEDVKTMMEALDHEYNNRESNNAEIEGLFEEELSL--------GEVKEAFDLFDEN 183
           +KL I+   T +   D E  +R  N+       E++L L         ++KEAFD+FDEN
Sbjct: 81  VKLHIKPENTGLRFEDFETLHRSLNDVFFGSKCEDKLGLNPDPAQDESDLKEAFDVFDEN 140

Query: 184 GDGFIDAEDLKKVLCGMGFAVASEVDQCRRMISGFDNNGDGRLDFEDFAKLV 228
           GDGFI A++L+ VL  +G    SE+D+   MIS  + + DGR+DF +F  ++
Sbjct: 141 GDGFISAKELQVVLEKLGLPEGSEIDRVEMMISSVEQDHDGRVDFFEFKDMM 192

[Close]

BLAST of CmUC04G079450 vs. ExPASy TrEMBL
Match: A0A6J1KID5 (probable calcium-binding protein CML47 OS=Cucurbita maxima OX=3661 GN=LOC111495509 PE=4 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 1.5e-87
Identity = 172/219 (78.54%), Postives = 189/219 (86.30%), Query Frame = 0


Query: 19  ISSFTLSKMAPIDELDNASPISVFQILFLIFLNFVLERFFSGFLYIFILCAAFLFYLWNS 78
           +  FTLS+MAPIDELD A+PISVFQILFL FLNF+LE  F G LY+FILCAAF FYLWNS
Sbjct: 4   VHPFTLSQMAPIDELDYANPISVFQILFLFFLNFLLEYLFPGPLYVFILCAAFHFYLWNS 63

Query: 79  WAAHRNIIKRLEAV-DATCTPSVRPNAHVGHCYTEIVGMPVNDTQELKLSIEDVKTMMEA 138
           W AHRNIIKR EA  DATCTPS R NAH+GHCYT IVGMPVND  E+KL+IEDVKTMM+A
Sbjct: 64  WVAHRNIIKRFEAADDATCTPSARRNAHMGHCYTGIVGMPVNDAHEVKLTIEDVKTMMDA 123

Query: 139 LDHEYNNRESNN----AEIEGLFEEELSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGMG 198
           L   +NN E+NN    AEIEG+FEEELS+GEV+EAFDLFDENGDGFIDAEDLKKVL  MG
Sbjct: 124 L---HNNMETNNLELKAEIEGVFEEELSVGEVEEAFDLFDENGDGFIDAEDLKKVLDAMG 183

Query: 199 FAVASEVDQCRRMISGFDNNGDGRLDFEDFAKLVEQSFC 233
           F VASEVD+CRRMISG+D NGDGRLDFE+FAKLVEQSFC
Sbjct: 184 FTVASEVDECRRMISGYDKNGDGRLDFEEFAKLVEQSFC 219

[Close]

BLAST of CmUC04G079450 vs. ExPASy TrEMBL
Match: A0A5A7T3X0 (Putative calcium-binding protein CML30 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00940 PE=4 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 2.0e-84
Identity = 163/211 (77.25%), Postives = 182/211 (86.26%), Query Frame = 0


Query: 27  MAPIDELDNASPISVFQILFLIFLNFVLERFFSGFLYIFILCAAFLFYLWNSWAAHRNII 86
           MAP D +D  +PISVF IL L FLNF+LERFF G  YIFIL AAFLFYLWNSWAA RNII
Sbjct: 1   MAPTDVIDYTNPISVFHILCLFFLNFILERFFPGLFYIFILYAAFLFYLWNSWAAQRNII 60

Query: 87  KRLEAVDATCTPSVRPNAHVGHCYTEIVGMPVNDTQELKLSIEDVKTMMEALDHEY-NNR 146
           +RLEA D TCTPSVR N H  HC T+IVG PVND +E+KLSIEDVKTMME LDHE+ NNR
Sbjct: 61  RRLEAADVTCTPSVRQNDHEEHCCTKIVGTPVNDKREVKLSIEDVKTMMETLDHEHNNNR 120

Query: 147 ESNN----AEIEGLFEEELSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGMGFAVASEVD 206
           +S+N    AE+E +FEEELSLGEV+EAFDLFDENGDGFIDAEDL+KVLCG+GFAVAS++D
Sbjct: 121 DSDNLGLSAELEEMFEEELSLGEVQEAFDLFDENGDGFIDAEDLEKVLCGLGFAVASDID 180

Query: 207 QCRRMISGFDNNGDGRLDFEDFAKLVEQSFC 233
           QC+ MISGFDNNGDGRLDFE+FAKLVEQSFC
Sbjct: 181 QCKTMISGFDNNGDGRLDFEEFAKLVEQSFC 211

[Close]

BLAST of CmUC04G079450 vs. ExPASy TrEMBL
Match: A0A1S3BEM1 (probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103488781 PE=4 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 1.3e-83
Identity = 162/211 (76.78%), Postives = 181/211 (85.78%), Query Frame = 0


Query: 27  MAPIDELDNASPISVFQILFLIFLNFVLERFFSGFLYIFILCAAFLFYLWNSWAAHRNII 86
           MAP D +D  +PISVF IL L FLNF+LERFF G  YIFIL AAFLFYLWNSWAA RNII
Sbjct: 1   MAPTDVIDYTNPISVFHILCLFFLNFILERFFPGLFYIFILYAAFLFYLWNSWAAQRNII 60

Query: 87  KRLEAVDATCTPSVRPNAHVGHCYTEIVGMPVNDTQELKLSIEDVKTMMEALDHEY-NNR 146
           +RLEA D TCTPSVR N H  HC T+IVG PVND +E+KLSIEDVKTMME LDHE+ NNR
Sbjct: 61  RRLEAADVTCTPSVRQNDHEEHCCTKIVGTPVNDKREVKLSIEDVKTMMETLDHEHNNNR 120

Query: 147 ESNN----AEIEGLFEEELSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGMGFAVASEVD 206
           +S+N    AE+E +FEEELSLGEV+EAFDLFDEN DGFIDAEDL+KVLCG+GFAVAS++D
Sbjct: 121 DSDNLGLSAELEEMFEEELSLGEVQEAFDLFDENDDGFIDAEDLEKVLCGLGFAVASDID 180

Query: 207 QCRRMISGFDNNGDGRLDFEDFAKLVEQSFC 233
           QC+ MISGFDNNGDGRLDFE+FAKLVEQSFC
Sbjct: 181 QCKTMISGFDNNGDGRLDFEEFAKLVEQSFC 211

[Close]

BLAST of CmUC04G079450 vs. ExPASy TrEMBL
Match: A0A6J1ECX5 (probable calcium-binding protein CML45 OS=Cucurbita moschata OX=3662 GN=LOC111431403 PE=4 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 9.8e-71
Identity = 140/172 (81.40%), Postives = 150/172 (87.21%), Query Frame = 0


Query: 66  ILCAAFLFYLWNSWAAHRNIIKRLEAVD-ATCTPSVRPNAHVGHCYTEIVGMPVNDTQEL 125
           +LCAAF FYLWNSW AHRNIIKR EA D ATCTPS R NAHVGHCYT IVGMPVND  E+
Sbjct: 1   MLCAAFHFYLWNSWVAHRNIIKRFEASDGATCTPSARRNAHVGHCYTGIVGMPVNDAHEV 60

Query: 126 KLSIEDVKTMMEALDHEYNNRESNN----AEIEGLFEEELSLGEVKEAFDLFDENGDGFI 185
           KL+IEDVKTMMEAL   +NN E+NN    AEIEG+FEEELS+GEVKEAFDLFDENGDGFI
Sbjct: 61  KLTIEDVKTMMEAL---HNNMETNNLELKAEIEGVFEEELSVGEVKEAFDLFDENGDGFI 120

Query: 186 DAEDLKKVLCGMGFAVASEVDQCRRMISGFDNNGDGRLDFEDFAKLVEQSFC 233
           DAEDLKKVL  MGF VASEVD+CRRMISG+D NGDGRLDFE+FAKLVEQSFC
Sbjct: 121 DAEDLKKVLDAMGFTVASEVDECRRMISGYDKNGDGRLDFEEFAKLVEQSFC 169

[Close]

BLAST of CmUC04G079450 vs. ExPASy TrEMBL
Match: A0A0A0LTE2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G033260 PE=4 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 2.4e-69
Identity = 146/214 (68.22%), Postives = 168/214 (78.50%), Query Frame = 0


Query: 27  MAPIDELDNASPISVFQILFLIFLNFVLERFFSGFLYIFILCAAFLFYLWNSWAAHRNII 86
           M PID +D  +PISVF ILFL FLNF+L RFF G   IFIL AAF+FYLWNSW A R+II
Sbjct: 1   MEPIDVVDYTNPISVFHILFLFFLNFILGRFFPGLFNIFILSAAFIFYLWNSWVAQRSII 60

Query: 87  KRLEAVDATCTPSVRPNAHVGH-CYTEIVGMPVND--TQELKLSIEDVKTMMEALDHEYN 146
                     TPSVR N H  H C T+IVGM VND   ++ KLSIEDVKTMME LDHE+N
Sbjct: 61  ----------TPSVRRNDHEEHCCCTKIVGMLVNDKRVEQAKLSIEDVKTMMEVLDHEHN 120

Query: 147 NR----ESNNAEIEGLFEEELSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGMG-FAVAS 206
           N     +S+N E+EG+FEEE+SLGEVKEAFDLFDENGDGFIDAEDLKKVLCG+G   V S
Sbjct: 121 NNNRDSDSDNLELEGMFEEEVSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGLGLITVGS 180

Query: 207 EVDQCRRMISGFDNNGDGRLDFEDFAKLVEQSFC 233
           ++D+C+RM+SGFDN+GDGRLDFE+FAKLVEQSFC
Sbjct: 181 DIDECKRMLSGFDNDGDGRLDFEEFAKLVEQSFC 204

[Close]

BLAST of CmUC04G079450 vs. TAIR 10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 97.1 bits (240), Expect = 2.0e-20
Identity = 56/129 (43.41%), Postives = 78/129 (60.47%), Query Frame = 0


Query: 111 TEIVGMPVNDTQELKLSIEDVKTMMEAL------DHEYNNRESNNAEIEGLFEE-ELSLG 170
           T I      D  +  L  EDV  +M++L      ++E   ++ ++ E+  LFEE E SL 
Sbjct: 76  TSIKHEEYRDDDDDGLCREDVGMVMKSLGLSTDQENEGLQKQYSSKEVSNLFEEKEPSLE 135

Query: 171 EVKEAFDLFDENGDGFIDAEDLKKVLCGMGFAVASEVDQCRRMISGFDNNGDGRLDFEDF 230
           EVK+AFD+FDEN DGFID  DL++VL  +G    S ++ CRRMI  FD + DGR+DF  F
Sbjct: 136 EVKQAFDVFDENRDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDFYGF 195

Query: 231 AKLVEQSFC 233
            K +E +FC
Sbjct: 196 VKFMENNFC 204

[Close]

BLAST of CmUC04G079450 vs. TAIR 10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 95.1 bits (235), Expect = 7.8e-20
Identity = 52/118 (44.07%), Postives = 75/118 (63.56%), Query Frame = 0


Query: 119 NDTQELKLSIEDVKTMMEALDHEYNN----RESNNAEIEGLFEE-ELSLGEVKEAFDLFD 178
           +D  ++ +S E+ + +M +L   YN+     + +  E+  LFEE E SL EVK+AFD+FD
Sbjct: 76  DDDDDIDISREEAEMVMRSLGLFYNDDQLQEQYSAKEVSSLFEEKEASLEEVKQAFDVFD 135

Query: 179 ENGDGFIDAEDLKKVLCGMGFAVASEVDQCRRMISGFDNNGDGRLDFEDFAKLVEQSF 232
           EN DGFIDA +L++VL  +GF   S +D C  MI   D N DG++DF +F K +E SF
Sbjct: 136 ENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMETSF 193

[Close]

BLAST of CmUC04G079450 vs. TAIR 10
Match: AT3G47480.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 76.3 bits (186), Expect = 3.7e-14
Identity = 63/200 (31.50%), Postives = 105/200 (52.50%), Query Frame = 0


Query: 37  SPISVFQI---LFLIFLNFVLERFFSGFLYIFILCAAFLFYLWNSWAAHRNIIKRLEAVD 96
           SPIS+  I   LF++ L  +++ F S F + F L  +  + L+ S   ++          
Sbjct: 8   SPISLLTIVIFLFILNLMMIIQDFSSSFPFRFHLFFSNAYILFTSIRNNK---------Q 67

Query: 97  ATCTPSVRPNAHVGHCYTEIVGMPVNDTQELKLSIEDVKTMMEALDHEYNNRESNNAEIE 156
            T  P ++              + V +  ++K S+E+VK +++  +  Y        E  
Sbjct: 68  NTELPIIKK-------------VVVPNRADIKTSVEEVKAIIDDSEALYECLIEEGEEY- 127

Query: 157 GLFEEELSLGE--VKEAFDLFDENGDGFIDAEDLKKVLCGMGFAVASEVDQCRRMISGFD 216
            L E+   +G+  VKEAF LFDEN DGFID  +LK VL  +G+   +++ +CR+M+  +D
Sbjct: 128 -LLEKNEMMGKEIVKEAFRLFDENQDGFIDENELKHVLSLLGYDECTKM-ECRKMVKVYD 182

Query: 217 NNGDGRLDFEDFAKLVEQSF 232
            N DG++DF +F KL+E+SF
Sbjct: 188 ENRDGKIDFYEFVKLIEKSF 182

[Close]

BLAST of CmUC04G079450 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 70.1 bits (170), Expect = 2.7e-12
Identity = 29/72 (40.28%), Postives = 51/72 (70.83%), Query Frame = 0


Query: 157 EEELSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGMGFAVASEVDQCRRMISGFDNNGDG 216
           EE+    ++KEAF++FD+NGDGFI  ++LK VL  +G      +D C++MI   D +GDG
Sbjct: 72  EEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDCKKMIKKVDVDGDG 131

Query: 217 RLDFEDFAKLVE 229
           R+++++F ++++
Sbjct: 132 RVNYKEFRQMMK 143

[Close]

BLAST of CmUC04G079450 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 70.1 bits (170), Expect = 2.7e-12
Identity = 29/73 (39.73%), Postives = 52/73 (71.23%), Query Frame = 0


Query: 157 EEELSLGEVKEAFDLFDENGDGFIDAEDLKKVLCGMGFAVASEVDQCRRMISGFDNNGDG 216
           E+E+   ++KEAF++FD NGDGFI  ++LK VL  +G      +++CR+MI   D +GDG
Sbjct: 74  EDEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECRKMIMQVDVDGDG 133

Query: 217 RLDFEDFAKLVEQ 230
           R+++ +F +++++
Sbjct: 134 RVNYMEFRQMMKK 146

The following BLAST results are available for this feature:

BLAST of CmUC04G079450 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038895011.1	1.2e-94	87.08	probable calcium-binding protein CML45 [Benincasa hispida]	[more]
XP_023519469.1	2.4e-87	79.00	probable calcium-binding protein CML47 [Cucurbita pepo subsp. pepo]	[more]
XP_023001351.1	3.1e-87	78.54	probable calcium-binding protein CML47 [Cucurbita maxima]	[more]
KAG7020054.1	7.7e-86	80.57	putative calcium-binding protein CML45, partial [Cucurbita argyrosperma subsp. a...	[more]
KAA0036089.1	4.2e-84	77.25	putative calcium-binding protein CML30 [Cucumis melo var. makuwa] >TYJ98897.1 pu...	[more]

BLAST of CmUC04G079450 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93Z27	2.9e-19	43.41	Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ...	[more]
Q9MBG5	1.1e-18	44.07	Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ...	[more]
Q9SN89	5.3e-13	31.50	Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 ...	[more]
Q2QVI1	9.9e-12	47.62	Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q09011	1.3e-11	37.50	Calcium-binding protein CAST OS=Solanum tuberosum OX=4113 PE=2 SV=1	[more]

BLAST of CmUC04G079450 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KID5	1.5e-87	78.54	probable calcium-binding protein CML47 OS=Cucurbita maxima OX=3661 GN=LOC1114955...	[more]
A0A5A7T3X0	2.0e-84	77.25	Putative calcium-binding protein CML30 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3BEM1	1.3e-83	76.78	probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103488781 P...	[more]
A0A6J1ECX5	9.8e-71	81.40	probable calcium-binding protein CML45 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A0A0LTE2	2.4e-69	68.22	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G033260 PE=4 SV=1	[more]

BLAST of CmUC04G079450 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G39670.1	2.0e-20	43.41	Calcium-binding EF-hand family protein	[more]
AT3G29000.1	7.8e-20	44.07	Calcium-binding EF-hand family protein	[more]
AT3G47480.1	3.7e-14	31.50	Calcium-binding EF-hand family protein	[more]
AT1G05990.1	2.7e-12	40.28	EF hand calcium-binding protein family	[more]
AT4G03290.1	2.7e-12	39.73	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 202..230 e-value: 0.0098 score: 25.1 coord: 164..192 e-value: 7.4E-5 score: 32.1
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 165..227 e-value: 3.2E-11 score: 43.5
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 198..233 score: 12.030714
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 160..195 score: 14.959732
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 164..227 e-value: 6.85232E-15 score: 65.2617
None	No IPR available	GENE3D	1.10.238.10		coord: 101..233 e-value: 1.4E-19 score: 72.7
None	No IPR available	PANTHER	PTHR10891:SF907	CALCIUM-BINDING PROTEIN CML47-RELATED	coord: 34..231
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 34..231
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 173..185
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 211..223
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 125..229

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmUC04G079450.1	CmUC04G079450.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006464	cellular protein modification process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005509	calcium ion binding