CmUC03G066680 (gene) Watermelon (USVL531) v1

Overview
NameCmUC03G066680
Typegene
OrganismCitrullus mucosospermus (Watermelon (USVL531) v1)
Descriptionamidase 1-like
LocationCmU531Chr03: 30780326 .. 30784756 (+)
RNA-Seq ExpressionCmUC03G066680
SyntenyCmUC03G066680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGACATTGGATTTTGATTTGATGTCGTTGAGAGAGGGGATAAGCCACAGACGATTCCGTTATACTATTTGCGACCCTTCTTCCTCATCTTCTTTGGTTGCCATAGCGTATTATCTTCCGCTCTGCAATTGTCCAGCCATTATTAGGGAGGTTGTGGCGGACTCTGCTTCCAATTAGATTCGGAGAAGGAGGGTGGAGAGCAAAAAACCTCAGGTTTTGCAATGGCTGTGCAGTATTATGGAGCTTTCATGGAGAAATTCCTCCTGCAACCGAGCTCCCCCTCCGATCAAATTCCCTTAACTGGCCTCACTTTCGCTGTTAAAGACATGTGAGATTCTTTTTATTATTTACTGTTTTTGATTTGGTTCTGGAAATTCATGAAGGATTTGGTGATAACAGGTCATGAGTTCTGTAATATTTCTAGTATTTTGGATGGTGTCAAAAGATTTCAATTTCAATGGTAGGTGGAAAATAGATGTTTCTCTCCGATGTTATTTCTAGGATCAACATATATATTCATGAAATGAATTGATTGTTCTTTGAGGAAAATTCATTAATTAGTAGTAAGATCAAATTACGACATTGGCCATCAATCAATCAATAAGTTACGAAAAAAGAAAAAAAAATCCAACAAAATGCTAAAAGTCAAGCAACATAAATGTCCAAAACCAATCACTAAAGTACTTGAATCTAACCATTAAGTAAAGCAAGTGACGAACTTTCTTAAAAGCCATCAACAGTTTGTTTTCTATTTTAATGGGTCATTAGTGTGTGCAAGATGAAGCCTAGTTCAACAAAATAATTCTTGGTTCTTTCGCTTCTTTGTTTACTTTAACGCGTAGTCCTTCAAATAAAAGTGTGTCAGTTTTTCTTCATGAAATAGGGGCATTGATGTCAAATTTCTCATTGGAATTTGAACAAAACAGATTTGATATGGATGGACATGTAACTGGTTTTGGAAATCCTGAATGGCTGAGGACTCACCCACCTGCCAATCAGACAGCCCCAGCCGTGTTGACCATCCTAAGAGGAGGAGCCACTTGCATTGGCAGGACTATCATGGATGAAATGGCCTACAGGTACCAAGAATACCTAACTTTTACTCTTTTTTTCCCCTTAGGTTTCTTATTATTTTCTTTTCTTTTACAATTAATAAACAACCATATAAGGCATAAGCTTTGCAAATTGGGATACAGTGGATTACTATTTTGTGCTTCACACCATTCTATAATTGGTCTTAAAGCTATGTCTATTCCATTCTTATCATTCTATTTCAAGCAGACAACTCTGCAATGTAATTGGTCAAAACATGAACTGAACCAATTAACTTTTGTCAGGTTTCTAATTTCTAGTTGAAAAGCTTTCACCAAATGATTTTTCCTGACAATGTAGGCTTTTAAATCTTTTCAATAGTGGATCACTAGTTTGGTAGTCGTATTTTCTCAGTATAAATGGGGAAAACTTTCACTATGGCACACCCCAAAACCCACATGCATCAGATCGGATACCTGGAGGATCTTCTAGTGGCTCCGCTGTTGCTGTAGGTGCAAAACTTGCGGATTTCTCCTTAGGCAAGTTCTTAGTTCTTTCAGAACAATTTGTGGTTTAATGACTTCTGCCTGGTAAATGTTGTAAAAGATCATATTTAGTTTTGTAGGAACTGATACTGGAGGTAGTGTAAGAGTGCCTGCATCCTATTGTGGAATACTTGGATTTCGGCCTTCACATGGTGCAGTCTCTACCTCCGGAGTAGTACCCATGGCACAGAGCTTTGATACAGTAGGTAATGAGTGGTGTCTCATTGTCACTTTGTCTACATCTTTTATAATCACGGACGACTTTGTTGCTCATGATTTTGCTTATATGAGCGATATAGTATTGGTTGGTAATACAGGTGTTGTTTGCTTATAAATTGAATAGAGTCCATCTCTCTCTCTCTCTCTCTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGCTATCTTTTTGAACATGTTATCTCTTCCTTTCAAGGCTTCAACATTGATTTTGCATTCATGATGTAACGCCATCTATTCTGCCATATTAAAAAGTGCATTGTTTGACACCACCTCCCCATTTTAGGATGGTTTGCCAGGGATCCTGTTATATTAAAAAAAGTAGGTCGGCTGCTGCTGGAACAGCCAGAGGTTGAATATCAAAAGCCTACACAGGTGCTCATTGCAGAAGATTGTTTCAAGCTGTCAAGCATTCCTAGTGAACGATTGACACAAGCTTTTGTTAATTCAGTAAAGAAGTTATTTGGTGGTGAGTCGACATTCCATATTAGAGGTTGTGTTTTGATGAATGTATATTTGATAATGGTTCATGAATGAATTGGATTTTGATTTCCTTTGATTTGAATTTCTCACTTGTTGAGTTTAGCAACACATGGGGTAGAAATTTGAATCTTTGACCTTTTGGTCGATGATATATGCTTTAACCATTTGAGTTATGCGTTGGTTGACATTCATTTTAGGCTTTTCATTTGCTCGAGAGGGAATTTATTAGAGGATCTAGCTTCATACTTTGAGATGTCTGCCATTTCTTAGACTATAGCCTTTTTGACACATTCAACAAAAGATCAATTCAGAGCCAAAGCTTTGCAAACGGTATACGGGATCATTGATAGTAATGTGGAAGGAAAATGATAAAACATAATATTTGAGTTCTTTTGTTCTTTATGATATTATTTTTCAACTGAGTTCGATGCTTTCTTATATGAGCCTTCAACTGCAGGCCATCTAATAAAACATGTCGACCTTGGGAATTATGTTGCGGACAAAGTTCCAAGTTTGAAGCATTTCATCATTGAAGGAAATGCAGGCCATGAGCATAGCATAGCATCCTTGGCAGCCCTAGCAAGATCGATGCAATTACTTCAGAGGTTCTGATCTCGCCACGAATTGTACTCAAATTTTGTCAAGCTGATTGATTATGATATATTTTTGTGTTGAATAAACGATTGATCTTCGTCCTACTTTCTTTGTACTTCCCCTTTGTAGGTATGAATTCAAAATCAATCATGAAGAATGGGTTAGGACTCACAATCCTCATTTGGGCCCAGGAATATCAGATCGAGTATCAGAAGCCATGAAGGCAACAGATGAGAATACTGATCTTTGTCATTCCATTAAAATGGAACTGCGTGAAGCTCTTGCTGCAATTCTTGAAGTACTCTCTCTCTCTCACACACGCTCTATTTACTAAAATTTCAAAGTAAGATAGTTAGCAACTTGAAATTCATTTTGCTTTTTGTTACAGAAAATTCTCAGTGACCAAAAATGTGATTTATGTGTGAAATGAATTACTTTTAAGGTCTAAATACTACGTTAATCTTTTACTTTTGATTTTAGTTCATTTTGGTTCTTATACTTTAAAAACATTTATTTTGGTTCCTATACTTTCAACTTTGGTTCATATACTTCTTTTGATCCTTGTATTGTTAACTTTGGTTTATTTTGGTTTTTATACTTTTAAAAAGTAACCATTTTGGTTATATATATAACAATCATATATTACACGGCAAAATTTGTTCCAAAATTATAGCGGCATATTAGAGTCGGTAAGAACAATGGAACCTCCATCTATCTCACAATGGTATGATATTTCACTTCAGGCATAAACCCTCGTGGCTTAGCTTTTGGAAGCACCCAAAAGAATGAATTGTTCTTGCTTATATATCCATGACCACTTCCTTCCCTAGCTGCTAATGTGGGACTTTTTTTACACTTCTAACAAGAGTTTGTTGAAGACTTTTTCTGTCGTTTCTATCTTACGAGACATACTCTGGAAAAAGCAGTTTGTGAATCATAGTTTTAAACTAATCTTGAGCTACTATTTGGATGATTTCATTTCAGGATTTTGGGGTCCTTGCAATTCCTACAGTCCCAGGCCCCCCTCCAAAACTAAACACAGACGTCTTGAAGCTACACGACTTTCGTGCGAAGGCTTTCAGCTTGCTCTCCATTGCTGGGGTATCCGGATTCTGCCAGGTTCTCTCTTTCGCTCTCTTAAAACTGAAAAGCAACCACTATGATGTTTTTTTTTTTCCTGACTAAGTAATCTGCAGGTTAGCATACCTCTAGGCTTGTACAATGGTCTTCCTGTATCAATATCTTTGCTAGCAAACCATGGTTCAGATGGGTTTTTGCTCAATGTTGTTCATAGTCTTTACAACACCTTAGAAGAAGAAGTTAAGGCAAGCTCCTAAAGAAAGACAAAAGGATGCTAAAGGAAACTACCTTGATATTTGAAATTTGCTGTTCTATTTTCAGATTGCAAGAATGGGAACATATGTAAGGTAGGTATTATCCTATATATTGATTTTTTTTTTTTGAGCAGTGAATAAATTTTACATTTTTGGATGATGAGATATTTGTTTTTAAGAATAGCCGTTGATAATGAATCT

mRNA sequence

TGGACATTGGATTTTGATTTGATGTCGTTGAGAGAGGGGATAAGCCACAGACGATTCCGTTATACTATTTGCGACCCTTCTTCCTCATCTTCTTTGGTTGCCATAGCGTATTATCTTCCGCTCTGCAATTGTCCAGCCATTATTAGGGAGGTTGTGGCGGACTCTGCTTCCAATTAGATTCGGAGAAGGAGGGTGGAGAGCAAAAAACCTCAGGTTTTGCAATGGCTGTGCAGTATTATGGAGCTTTCATGGAGAAATTCCTCCTGCAACCGAGCTCCCCCTCCGATCAAATTCCCTTAACTGGCCTCACTTTCGCTGTTAAAGACATATTTGATATGGATGGACATGTAACTGGTTTTGGAAATCCTGAATGGCTGAGGACTCACCCACCTGCCAATCAGACAGCCCCAGCCGTGTTGACCATCCTAAGAGGAGGAGCCACTTGCATTGGCAGGACTATCATGGATGAAATGGCCTACAGTATAAATGGGGAAAACTTTCACTATGGCACACCCCAAAACCCACATGCATCAGATCGGATACCTGGAGGATCTTCTAGTGGCTCCGCTGTTGCTGTAGGAACTGATACTGGAGGTAGTGTAAGAGTGCCTGCATCCTATTGTGGAATACTTGGATTTCGGCCTTCACATGGTGCAGTCTCTACCTCCGGAGTAGTACCCATGGCACAGAGCTTTGATACAGTAGGTAATGAGTGGTGTCTCATTGTCACTTTGTCTACATCTTTTATAATCACGGACGACTTTGTTGCTCATGATTTTGCTTATATGAGCGATATAGTATTGGTTGGTAATACAGGATGGTTTGCCAGGGATCCTGTTATATTAAAAAAAGTAGGTCGGCTGCTGCTGGAACAGCCAGAGGTTGAATATCAAAAGCCTACACAGGTGCTCATTGCAGAAGATTGTTTCAAGCTGTCAAGCATTCCTAGTGAACGATTGACACAAGCTTTTGTTAATTCAGTAAAGAAGTTATTTGGTGGCCATCTAATAAAACATGTCGACCTTGGGAATTATGTTGCGGACAAAGTTCCAAGTTTGAAGCATTTCATCATTGAAGGAAATGCAGGCCATGAGCATAGCATAGCATCCTTGGCAGCCCTAGCAAGATCGATGCAATTACTTCAGAGGTATGAATTCAAAATCAATCATGAAGAATGGGTTAGGACTCACAATCCTCATTTGGGCCCAGGAATATCAGATCGAGTATCAGAAGCCATGAAGGCAACAGATGAGAATACTGATCTTTGTCATTCCATTAAAATGGAACTGCGTGAAGCTCTTGCTGCAATTCTTGAAGATTTTGGGGTCCTTGCAATTCCTACAGTCCCAGGCCCCCCTCCAAAACTAAACACAGACGTCTTGAAGCTACACGACTTTCGTGCGAAGGCTTTCAGCTTGCTCTCCATTGCTGGGGTATCCGGATTCTGCCAGGTTAGCATACCTCTAGGCTTGTACAATGGTCTTCCTGTATCAATATCTTTGCTAGCAAACCATGGTTCAGATGGGTTTTTGCTCAATGTTGTTCATAGTCTTTACAACACCTTAGAAGAAGAAGTTAAGGCAAGCTCCTAAAGAAAGACAAAAGGATGCTAAAGGAAACTACCTTGATATTTGAAATTTGCTGTTCTATTTTCAGATTGCAAGAATGGGAACATATGTAAGGTAGGTATTATCCTATATATTGATTTTTTTTTTTTGAGCAGTGAATAAATTTTACATTTTTGGATGATGAGATATTTGTTTTTAAGAATAGCCGTTGATAATGAATCT

Coding sequence (CDS)

ATGGCTGTGCAGTATTATGGAGCTTTCATGGAGAAATTCCTCCTGCAACCGAGCTCCCCCTCCGATCAAATTCCCTTAACTGGCCTCACTTTCGCTGTTAAAGACATATTTGATATGGATGGACATGTAACTGGTTTTGGAAATCCTGAATGGCTGAGGACTCACCCACCTGCCAATCAGACAGCCCCAGCCGTGTTGACCATCCTAAGAGGAGGAGCCACTTGCATTGGCAGGACTATCATGGATGAAATGGCCTACAGTATAAATGGGGAAAACTTTCACTATGGCACACCCCAAAACCCACATGCATCAGATCGGATACCTGGAGGATCTTCTAGTGGCTCCGCTGTTGCTGTAGGAACTGATACTGGAGGTAGTGTAAGAGTGCCTGCATCCTATTGTGGAATACTTGGATTTCGGCCTTCACATGGTGCAGTCTCTACCTCCGGAGTAGTACCCATGGCACAGAGCTTTGATACAGTAGGTAATGAGTGGTGTCTCATTGTCACTTTGTCTACATCTTTTATAATCACGGACGACTTTGTTGCTCATGATTTTGCTTATATGAGCGATATAGTATTGGTTGGTAATACAGGATGGTTTGCCAGGGATCCTGTTATATTAAAAAAAGTAGGTCGGCTGCTGCTGGAACAGCCAGAGGTTGAATATCAAAAGCCTACACAGGTGCTCATTGCAGAAGATTGTTTCAAGCTGTCAAGCATTCCTAGTGAACGATTGACACAAGCTTTTGTTAATTCAGTAAAGAAGTTATTTGGTGGCCATCTAATAAAACATGTCGACCTTGGGAATTATGTTGCGGACAAAGTTCCAAGTTTGAAGCATTTCATCATTGAAGGAAATGCAGGCCATGAGCATAGCATAGCATCCTTGGCAGCCCTAGCAAGATCGATGCAATTACTTCAGAGGTATGAATTCAAAATCAATCATGAAGAATGGGTTAGGACTCACAATCCTCATTTGGGCCCAGGAATATCAGATCGAGTATCAGAAGCCATGAAGGCAACAGATGAGAATACTGATCTTTGTCATTCCATTAAAATGGAACTGCGTGAAGCTCTTGCTGCAATTCTTGAAGATTTTGGGGTCCTTGCAATTCCTACAGTCCCAGGCCCCCCTCCAAAACTAAACACAGACGTCTTGAAGCTACACGACTTTCGTGCGAAGGCTTTCAGCTTGCTCTCCATTGCTGGGGTATCCGGATTCTGCCAGGTTAGCATACCTCTAGGCTTGTACAATGGTCTTCCTGTATCAATATCTTTGCTAGCAAACCATGGTTCAGATGGGTTTTTGCTCAATGTTGTTCATAGTCTTTACAACACCTTAGAAGAAGAAGTTAAGGCAAGCTCCTAA

Protein sequence

MAVQYYGAFMEKFLLQPSSPSDQIPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANQTAPAVLTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPHASDRIPGGSSSGSAVAVGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGNEWCLIVTLSTSFIITDDFVAHDFAYMSDIVLVGNTGWFARDPVILKKVGRLLLEQPEVEYQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKHVDLGNYVADKVPSLKHFIIEGNAGHEHSIASLAALARSMQLLQRYEFKINHEEWVRTHNPHLGPGISDRVSEAMKATDENTDLCHSIKMELREALAAILEDFGVLAIPTVPGPPPKLNTDVLKLHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLLANHGSDGFLLNVVHSLYNTLEEEVKASS
Homology
BLAST of CmUC03G066680 vs. NCBI nr
Match: XP_038878397.1 (amidase 1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 758.1 bits (1956), Expect = 4.5e-215
Identity = 381/459 (83.01%), Postives = 401/459 (87.36%), Query Frame = 0

Query: 1   MAVQYYGAFMEKFLLQPSSPSDQIPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANQ 60
           MAVQYYGAFMEKFLLQPSSPSD++PL GLTFAVKDIFDMDGHVTGFGNPEWLRTH PANQ
Sbjct: 1   MAVQYYGAFMEKFLLQPSSPSDELPLNGLTFAVKDIFDMDGHVTGFGNPEWLRTHRPANQ 60

Query: 61  TAPAVLTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPHASDRIPGGSSSGSAVAV- 120
           TAPAVLTILRGGA+CIGRTIMDEMAYSINGENFHYGTPQNP+A DR+PGGSSSGSAVAV 
Sbjct: 61  TAPAVLTILRGGASCIGRTIMDEMAYSINGENFHYGTPQNPYALDRVPGGSSSGSAVAVG 120

Query: 121 --------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGNEWCLIVTL 180
                   GTDTGGS+RVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTV          
Sbjct: 121 AKLVDFSLGTDTGGSIRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTV---------- 180

Query: 181 STSFIITDDFVAHDFAYMSDIVLVGNTGWFARDPVILKKVGRLLLEQPEVEYQKPTQVLI 240
                                      GWFARDPV+LKKVGRLLL+ PEVE+QKPTQVLI
Sbjct: 181 ---------------------------GWFARDPVVLKKVGRLLLQHPEVEHQKPTQVLI 240

Query: 241 AEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKHVDLGNYVADKVPSLKHFIIEGNAGHE 300
           AEDCFKLSSIPSERLTQAF+NSVKKLFGGHLIK V LG Y+ DKVPSLKHF+IEGNAGHE
Sbjct: 241 AEDCFKLSSIPSERLTQAFINSVKKLFGGHLIKQVVLGTYLVDKVPSLKHFMIEGNAGHE 300

Query: 301 HSIASLAALARSMQLLQRYEFKINHEEWVRTHNPHLGPGISDRVSEAMKATDENTDLCHS 360
           HSI SLAALARSMQLLQRYEFKINHEEWVRT+NPHLGPGIS+RVSEAM+AT+EN DLCH+
Sbjct: 301 HSIPSLAALARSMQLLQRYEFKINHEEWVRTYNPHLGPGISERVSEAMRATNENIDLCHN 360

Query: 361 IKMELREALAAILEDFGVLAIPTVPGPPPKLNTDVLKLHDFRAKAFSLLSIAGVSGFCQV 420
           IKMELREALAA+LE+FGVLAIPTVPGPPPKLNTD+ KLHDFRAKAFSLLSIAGVSGFCQV
Sbjct: 361 IKMELREALAALLEEFGVLAIPTVPGPPPKLNTDISKLHDFRAKAFSLLSIAGVSGFCQV 420

Query: 421 SIPLGLYNGLPVSISLLANHGSDGFLLNVVHSLYNTLEE 451
           SIPLGLYNGLPVSISLLA HGSDGFLLNVVHSLYNTLEE
Sbjct: 421 SIPLGLYNGLPVSISLLAKHGSDGFLLNVVHSLYNTLEE 422

BLAST of CmUC03G066680 vs. NCBI nr
Match: XP_008450658.1 (PREDICTED: amidase 1-like isoform X2 [Cucumis melo])

HSP 1 Score: 758.1 bits (1956), Expect = 4.5e-215
Identity = 387/464 (83.41%), Postives = 404/464 (87.07%), Query Frame = 0

Query: 1   MAVQYYGAFMEKFLLQPSSPSDQIPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANQ 60
           MAVQ YGAFMEKFLLQPSSPSDQ+PLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPAN 
Sbjct: 1   MAVQ-YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60

Query: 61  TAPAVLTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPHASDRIPGGSSSGSAVAV- 120
           TAP V TILRGGATCIGRTIMDEMAYSINGENFHYGTPQNP ASDR+PGGSSSGSAVAV 
Sbjct: 61  TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120

Query: 121 --------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGNEWCLIVTL 180
                   GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTV          
Sbjct: 121 ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTV---------- 180

Query: 181 STSFIITDDFVAHDFAYMSDIVLVGNTGWFARDPVILKKVGRLLLEQPEVEYQKPTQVLI 240
                                      GWFARDPV+ KKVG LLL+QPEVE+QKPTQVLI
Sbjct: 181 ---------------------------GWFARDPVVFKKVGLLLLQQPEVEHQKPTQVLI 240

Query: 241 AEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKHVDLGNYVADKVPSLKHFIIEGNAGHE 300
           AEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIK V+LGNYV +KVPSLK F+IEGNAG+E
Sbjct: 241 AEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLGNYVEEKVPSLKQFMIEGNAGNE 300

Query: 301 HSIASLAALARSMQLLQRYEFKINHEEWVRTHNPHLGPGISDRVSEAMKATDENTDLCHS 360
           HSI SLAALARS+QLLQRYEFKINHEEWVRT+NPHLGPGIS+RVSEAM+ATDEN DLCHS
Sbjct: 301 HSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGPGISERVSEAMRATDENIDLCHS 360

Query: 361 IKMELREALAAILEDFGVLAIPTVPGPPPKLNTDVLKLHDFRAKAFSLLSIAGVSGFCQV 420
           IK+ELR ALAA+LEDFGVLAIPTVPGPPPKLNTDV +LHDFRAKAFSLLSIAGVSGFCQV
Sbjct: 361 IKIELRRALAALLEDFGVLAIPTVPGPPPKLNTDVSELHDFRAKAFSLLSIAGVSGFCQV 420

Query: 421 SIPLGLYNGLPVSISLLANHGSDGFLLNVVHSLYNTLEEEVKAS 456
           SIPLGLYNGLPVSISL+ANHGSDGFLLNVVHSLYNTLEEEVKAS
Sbjct: 421 SIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNTLEEEVKAS 426

BLAST of CmUC03G066680 vs. NCBI nr
Match: TYK10232.1 (amidase 1-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 756.5 bits (1952), Expect = 1.3e-214
Identity = 387/468 (82.69%), Postives = 404/468 (86.32%), Query Frame = 0

Query: 1   MAVQYYGAFMEKFLLQPSSPSDQIPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANQ 60
           MAVQ YGAFMEKFLLQPSSPSDQ+PLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPAN 
Sbjct: 46  MAVQ-YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 105

Query: 61  TAPAVLTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPHASDRIPGGSSSGSAVA-- 120
           TAP V TILRGGATCIGRTIMDEMAYSINGENFHYGTPQNP ASDR+PGGSSSGSAVA  
Sbjct: 106 TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 165

Query: 121 -----------VGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGNEWCL 180
                      VGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTV      
Sbjct: 166 ANLVDFSLGTFVGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTV------ 225

Query: 181 IVTLSTSFIITDDFVAHDFAYMSDIVLVGNTGWFARDPVILKKVGRLLLEQPEVEYQKPT 240
                                          GWFARDPV+ KKVG LLL+QPEVE+QKPT
Sbjct: 226 -------------------------------GWFARDPVVFKKVGLLLLQQPEVEHQKPT 285

Query: 241 QVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKHVDLGNYVADKVPSLKHFIIEGN 300
           QVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIK V+LGNYV +KVPSLK F+IEGN
Sbjct: 286 QVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLGNYVEEKVPSLKQFMIEGN 345

Query: 301 AGHEHSIASLAALARSMQLLQRYEFKINHEEWVRTHNPHLGPGISDRVSEAMKATDENTD 360
           AG+EHSI SLAALARS+QLLQRYEFKINHEEWVRT+NPHLGPGIS+RVSEAM+ATDEN D
Sbjct: 346 AGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGPGISERVSEAMRATDENID 405

Query: 361 LCHSIKMELREALAAILEDFGVLAIPTVPGPPPKLNTDVLKLHDFRAKAFSLLSIAGVSG 420
           LCHSIK+ELR ALAA+LEDFGVLAIPTVPGPPPKLNTDV +LHDFRAKAFSLLSIAGVSG
Sbjct: 406 LCHSIKIELRRALAALLEDFGVLAIPTVPGPPPKLNTDVSELHDFRAKAFSLLSIAGVSG 465

Query: 421 FCQVSIPLGLYNGLPVSISLLANHGSDGFLLNVVHSLYNTLEEEVKAS 456
           FCQVSIPLGLYNGLPVSISL+ANHGSDGFLLNVVHSLYNTLEEEVKAS
Sbjct: 466 FCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNTLEEEVKAS 475

BLAST of CmUC03G066680 vs. NCBI nr
Match: XP_004135624.1 (amidase 1 [Cucumis sativus] >KGN66086.1 hypothetical protein Csa_006860 [Cucumis sativus])

HSP 1 Score: 754.6 bits (1947), Expect = 5.0e-214
Identity = 384/464 (82.76%), Postives = 402/464 (86.64%), Query Frame = 0

Query: 1   MAVQYYGAFMEKFLLQPSSPSDQIPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANQ 60
           MAVQ YGAFMEKFLLQPSSPSDQ+PLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPAN 
Sbjct: 1   MAVQCYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60

Query: 61  TAPAVLTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPHASDRIPGGSSSGSAVAV- 120
           TAP V TILRGGATCIGRTIMDEMAYSINGENFHYGTPQNP ASDR+PGGSSSGSAVAV 
Sbjct: 61  TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120

Query: 121 --------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGNEWCLIVTL 180
                   GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQS DTV          
Sbjct: 121 ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSMDTV---------- 180

Query: 181 STSFIITDDFVAHDFAYMSDIVLVGNTGWFARDPVILKKVGRLLLEQPEVEYQKPTQVLI 240
                                      GWFARDPV+LKKVG LLL+QPEVE+QKP QVLI
Sbjct: 181 ---------------------------GWFARDPVVLKKVGWLLLQQPEVEHQKPRQVLI 240

Query: 241 AEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKHVDLGNYVADKVPSLKHFIIEGNAGHE 300
           AEDCFKLSSIPSERLTQAFVNSVKKLFGGH IK V LGNYV +KVPSLKHF+IEGNAG+E
Sbjct: 241 AEDCFKLSSIPSERLTQAFVNSVKKLFGGHPIKEVSLGNYVEEKVPSLKHFMIEGNAGNE 300

Query: 301 HSIASLAALARSMQLLQRYEFKINHEEWVRTHNPHLGPGISDRVSEAMKATDENTDLCHS 360
           HSI SLAALARS+QLLQRYEFKINHEEWVRT+NPHLGPGIS+RVSEAM+ATDEN DLCHS
Sbjct: 301 HSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGPGISERVSEAMRATDENIDLCHS 360

Query: 361 IKMELREALAAILEDFGVLAIPTVPGPPPKLNTDVLKLHDFRAKAFSLLSIAGVSGFCQV 420
           I++ELR+ALAA+LEDFGVLAIPTVPGPPPKLNTDV +L DFRAKAFSLLSIAGVSGFCQV
Sbjct: 361 IRIELRKALAALLEDFGVLAIPTVPGPPPKLNTDVSELQDFRAKAFSLLSIAGVSGFCQV 420

Query: 421 SIPLGLYNGLPVSISLLANHGSDGFLLNVVHSLYNTLEEEVKAS 456
           SIPLGLYNGLPVSISL+ANHGSDGFLLNVVHSLYNTLEEEVKAS
Sbjct: 421 SIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNTLEEEVKAS 427

BLAST of CmUC03G066680 vs. NCBI nr
Match: KAA0050880.1 (amidase 1-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 753.4 bits (1944), Expect = 1.1e-213
Identity = 386/468 (82.48%), Postives = 403/468 (86.11%), Query Frame = 0

Query: 1   MAVQYYGAFMEKFLLQPSSPSDQIPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANQ 60
           MAVQ YGAFMEKFLLQPSSPSDQ+PLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPAN 
Sbjct: 46  MAVQ-YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 105

Query: 61  TAPAVLTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPHASDRIPGGSSSGSAVA-- 120
           TAP V TILRGGATCIGRTIMDEMAYSINGENFHYGTPQNP ASDR+PGGSSSGSAVA  
Sbjct: 106 TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 165

Query: 121 -----------VGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGNEWCL 180
                      VGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTV      
Sbjct: 166 ANLVDFSLGTFVGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTV------ 225

Query: 181 IVTLSTSFIITDDFVAHDFAYMSDIVLVGNTGWFARDPVILKKVGRLLLEQPEVEYQKPT 240
                                          GWFARDPV+ KKVG LLL+QPEVE+QKPT
Sbjct: 226 -------------------------------GWFARDPVVFKKVGLLLLQQPEVEHQKPT 285

Query: 241 QVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKHVDLGNYVADKVPSLKHFIIEGN 300
           QVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIK V+LGNYV +KVPSLK F+IEGN
Sbjct: 286 QVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLGNYVEEKVPSLKQFMIEGN 345

Query: 301 AGHEHSIASLAALARSMQLLQRYEFKINHEEWVRTHNPHLGPGISDRVSEAMKATDENTD 360
           AG+EHSI SLAALARS+QLLQRYEFKINHEEWVRT+NPHLGPGIS+RVSEAM+ATDEN D
Sbjct: 346 AGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGPGISERVSEAMRATDENID 405

Query: 361 LCHSIKMELREALAAILEDFGVLAIPTVPGPPPKLNTDVLKLHDFRAKAFSLLSIAGVSG 420
           LCHSIK+ELR ALAA+LEDFGVLAIPTVPGPPPKLNTDV +LHDFRAKAFSLLSIAGVSG
Sbjct: 406 LCHSIKIELRRALAALLEDFGVLAIPTVPGPPPKLNTDVSELHDFRAKAFSLLSIAGVSG 465

Query: 421 FCQVSIPLGLYNGLPVSISLLANHGSDGFLLNVVHSLYNTLEEEVKAS 456
           FCQVSIPL LYNGLPVSISL+ANHGSDGFLLNVVHSLYNTLEEEVKAS
Sbjct: 466 FCQVSIPLRLYNGLPVSISLVANHGSDGFLLNVVHSLYNTLEEEVKAS 475

BLAST of CmUC03G066680 vs. ExPASy Swiss-Prot
Match: Q9FR37 (Amidase 1 OS=Arabidopsis thaliana OX=3702 GN=AMI1 PE=1 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 5.1e-137
Identity = 257/452 (56.86%), Postives = 313/452 (69.25%), Query Frame = 0

Query: 6   YGAFMEKFLLQPSSPSDQIP-LTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANQTAPA 65
           +GAF+EK  + P+S S   P L GLTFA+KDIFD++G VTGFGNP+WLRTH  A  TAP 
Sbjct: 7   FGAFIEKVTISPTSTSSSPPSLQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTAPV 66

Query: 66  VLTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPHASDRIPGGSSSGSAVAV----- 125
           V ++L  GAT +G TIMDEMAYSINGEN HYGTP+NP A DR+PGGSSSGSAVAV     
Sbjct: 67  VSSLLEAGATALGITIMDEMAYSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAARLV 126

Query: 126 ----GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGNEWCLIVTLSTSF 185
               GTDTGGSVRVPASYCGI GFRPSHGAVST G+ PMAQSFDTV              
Sbjct: 127 DFSIGTDTGGSVRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTV-------------- 186

Query: 186 IITDDFVAHDFAYMSDIVLVGNTGWFARDPVILKKVGRLLLEQPEVEYQKPTQVLIAEDC 245
                                  GWFARD   LK+VG +LL+Q  +   +P+Q++IA+DC
Sbjct: 187 -----------------------GWFARDTATLKRVGCVLLQQHHLNPIEPSQLIIADDC 246

Query: 246 FKLSSIPSERLTQAFVNSVKKLFGGH-LIKHVDLGNYVADKVPSLKHFIIEGN--AGHEH 305
           FKL S+P + L Q  V SV+K FGG+ ++K V+LG Y+   VPSLKHF+   +     E 
Sbjct: 247 FKLCSVPHDLLVQPLVGSVEKSFGGNTVVKKVNLGEYIGQNVPSLKHFMTSDDVTTQQEF 306

Query: 306 SIASLAALARSMQLLQRYEFKINHEEWVRTHNPHLGPGISDRVSEAMKATDENTDLCHSI 365
            I SL AL+ SM+LLQR+EFKINH  W+ +  P  GPGIS+R+ EA++ +DE  D C S+
Sbjct: 307 CIPSLMALSSSMRLLQRHEFKINHGAWISSVKPEFGPGISERIEEAIRTSDEKIDHCRSV 366

Query: 366 KMELREALAAILEDFGVLAIPTVPGPPPKLNTDVLKLHDFRAKAFSLLSIAGVSGFCQVS 425
           K EL  AL+ +L + GVL IPTVPGPPP L  +V  L  FR++AFSLLSIAGVSGFCQVS
Sbjct: 367 KSELITALSTLLGEKGVLVIPTVPGPPPHLQANVAALESFRSRAFSLLSIAGVSGFCQVS 421

Query: 426 IPLGLYNGLPVSISLLANHGSDGFLLNVVHSL 445
           IPLGL+  LPVS+SL+A +GSDGFLL++V SL
Sbjct: 427 IPLGLHENLPVSVSLVAKYGSDGFLLSLVDSL 421

BLAST of CmUC03G066680 vs. ExPASy Swiss-Prot
Match: Q7XTK3 (Amidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMI1 PE=1 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 3.1e-126
Identity = 239/456 (52.41%), Postives = 302/456 (66.23%), Query Frame = 0

Query: 6   YGAFMEKFLLQPSSPSDQIPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANQTAPAV 65
           YGAFME+F+L P  PS Q+PL GLTFA+KDIFD+ G VTGFGNP+W RTH PA  T+P V
Sbjct: 11  YGAFMERFVL-PPPPSQQLPLHGLTFAIKDIFDIAGRVTGFGNPDWARTHAPAAATSPVV 70

Query: 66  LTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPHASDRIPGGSSSGSAVAV------ 125
           L  L  GAT +G TIMDEMAYSINGEN HYGTP NP A  R+PGGSSSGSAVAV      
Sbjct: 71  LAALAAGATSLGTTIMDEMAYSINGENTHYGTPTNPCAPGRVPGGSSSGSAVAVAANLVD 130

Query: 126 ---GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGNEWCLIVTLSTSFI 185
              GTDTGGSVRVPA+YCGI G RPSHG VS   V+PMAQ FDTV               
Sbjct: 131 FSLGTDTGGSVRVPAAYCGIFGLRPSHGLVSAENVIPMAQMFDTV--------------- 190

Query: 186 ITDDFVAHDFAYMSDIVLVGNTGWFARDPVILKKVGRLLLEQPEVEYQKPTQVLIAEDCF 245
                                 GWF+RD   L +V ++LL  P+   ++PTQV I  DCF
Sbjct: 191 ----------------------GWFSRDLSTLSRVTKVLLPLPDDIVKQPTQVTIPMDCF 250

Query: 246 KLSSIPSERLTQAFVNSVKKLFGGHLIKHVDLGNYVADKVPSLKHFIIEGNAGHEHSIAS 305
           ++     +R  Q    SV K F   ++ + +LG++++D VPS+  FI + +     S+ +
Sbjct: 251 QILGSLDDRTYQIINASVAKRFDSQILDNRNLGDFISDNVPSIGKFITDFSESELPSVPA 310

Query: 306 LAALARSMQLLQRYEFKINHEEWVRTHNPHLGPGISDRVSEAMKATD-ENTDLCHSIKME 365
           L+ ++  M+ LQR +FK NH EWV T  P+LGPG+ +R+ EA+ + D E+ +   +I+ E
Sbjct: 311 LSVISHVMRGLQRSQFKANHAEWVNTVKPNLGPGLRERILEAIASGDNESLEDFQAIRAE 370

Query: 366 LREALAAILEDFGVLAIPTVPGPPPKLNTDVLKLHDFRAKAFSLLSIAGVSGFCQVSIPL 425
            + ALAA+L+D G+LAIPTVPGPPPK+  +   L +FRA+AFSLLSIAG+SGFCQVSIPL
Sbjct: 371 FKSALAALLKDHGILAIPTVPGPPPKVGMEAAPLENFRARAFSLLSIAGLSGFCQVSIPL 428

Query: 426 GLYNGLPVSISLLANHGSDGFLLNVVHSLYNTLEEE 452
           G+ NGLPVS+SL+A HG+D FLLNVV  LY TL +E
Sbjct: 431 GMRNGLPVSVSLVARHGADHFLLNVVEELYQTLIDE 428

BLAST of CmUC03G066680 vs. ExPASy Swiss-Prot
Match: Q9LVH5 (Outer envelope protein 64, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP64 PE=1 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 1.5e-109
Identity = 202/458 (44.10%), Postives = 288/458 (62.88%), Query Frame = 0

Query: 6   YGAFMEKFLL----QPSSPSDQIPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANQT 65
           +GAF++K +L    QP+ P    PLTGLTFAV D+FD+ G+VTGFG+P+W+RTH  A+ T
Sbjct: 34  FGAFIDKLMLLPPPQPAPPKAPHPLTGLTFAVSDVFDITGYVTGFGHPDWVRTHEAASST 93

Query: 66  APAVLTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPHASDRIPGGSSSGSAVAV-- 125
           +P V T++ GGATC+G+T++DE A+SI+GEN HY +P NP A  RIPGG+ SG+AVAV  
Sbjct: 94  SPVVSTLVEGGATCVGKTVVDEFAFSISGENKHYDSPTNPAAPTRIPGGACSGAAVAVAT 153

Query: 126 -------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGNEWCLIVTLS 185
                  G DT G VRVPA YCG+LGF+ S+GA+S +G++P++ S D+V           
Sbjct: 154 NAVDFALGIDTVGGVRVPAGYCGVLGFKSSYGAISNTGIIPVSSSLDSV----------- 213

Query: 186 TSFIITDDFVAHDFAYMSDIVLVGNTGWFARDPVILKKVGRLLLEQPEVEYQKPTQVLIA 245
                                     GWFARDP  L++VG +LL+ P    + P Q+++A
Sbjct: 214 --------------------------GWFARDPNTLRRVGHVLLQLPFATQRNPRQIILA 273

Query: 246 EDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKHVDLGNYVADKVPSLKHFIIEGNAGHEH 305
           +DCF+L  IP +R+TQ    S +KLFG  L+KH +L  Y   KVPSLK F     A    
Sbjct: 274 DDCFQLLKIPVDRITQVVTKSAEKLFGRQLLKHQNLETYFETKVPSLKEF-ARTKAIANT 333

Query: 306 SIASLAALARSMQLLQRYEFKINHEEWVRTHNPHLGPGISDRVSEAMKATDENTDLCHSI 365
            +++   LA  MQLLQR+EF  NH +W+ T  P + P I  +V E  + T+E T+  ++I
Sbjct: 334 KVSTSRLLANVMQLLQRHEFLQNHGDWINTVKPAIDPVILSQVCENPELTNEETENLNAI 393

Query: 366 KMELREALAAILEDFGVLAIPTVPGPPPKLNTDVLKLHDFRAKAFSLLSIAGVSGFCQVS 425
           + E R A+ ++L+D G+L IPT+P  PPKL +  +   D++ +A SLLSIA +SG CQV+
Sbjct: 394 RNETRVAIGSLLKDDGILVIPTLPAVPPKLGSKEITSEDYQNRASSLLSIASISGCCQVT 453

Query: 426 IPLGLYNGLPVSISLLANHGSDGFLLNVVHSLYNTLEE 451
           +PLG +   P+S+S +  HG D FLL+ V ++Y +L+E
Sbjct: 454 VPLGHHEKCPISVSFIGRHGGDRFLLDTVQTMYPSLQE 453

BLAST of CmUC03G066680 vs. ExPASy Swiss-Prot
Match: Q9MUK5 (Translocon at the outer membrane of chloroplasts 64 OS=Pisum sativum OX=3888 GN=TOC64 PE=1 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 3.4e-109
Identity = 203/459 (44.23%), Postives = 289/459 (62.96%), Query Frame = 0

Query: 6   YGAFMEKFLL----QPSSPSDQIPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANQT 65
           +GAF+ K  L     P+ P    PL+ L FA+ DIFD++GHV+ FG+PEW RTH PA+ T
Sbjct: 37  FGAFLLKLKLLPPPPPAPPKAPHPLSSLNFAISDIFDIEGHVSTFGHPEWARTHEPASST 96

Query: 66  APAVLTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPHASDRIPGGSSSGSAVAV-- 125
           A AV  ++  GATCIG T++DE+AY I+GEN H+GTP NP   +R+PGGSSSG+AVAV  
Sbjct: 97  ASAVSALVESGATCIGTTVVDELAYGISGENKHFGTPTNPAVPNRVPGGSSSGAAVAVAA 156

Query: 126 -------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGNEWCLIVTLS 185
                  G DT G VRVPA +CGILGFRPSHGAVS  G++P++ S DTV           
Sbjct: 157 NFVDFSLGVDTSGGVRVPAGFCGILGFRPSHGAVSHVGIIPVSTSLDTV----------- 216

Query: 186 TSFIITDDFVAHDFAYMSDIVLVGNTGWFARDPVILKKVGRLLLEQPEVEYQKPTQVLIA 245
                                     GWFA+DP +L++VG +LL+ P V  + P Q++IA
Sbjct: 217 --------------------------GWFAKDPDVLRRVGHILLQAPFVMQRNPRQIIIA 276

Query: 246 EDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKHVDLGNYVADKVPSLKHFIIEGNAGHEH 305
           +DCF+  ++P +R +Q  + + +KLFG  ++KH++  +Y++ KV SLK   I+ + G   
Sbjct: 277 DDCFQHLNVPLDRTSQVVIKATEKLFGKQVLKHINFEDYISSKVSSLKACSIQKSNGVLK 336

Query: 306 SIASLAALARSMQLLQRYEFKINHEEWVRTHNPHLGPGISDRVSEAMKATDENTDLCHSI 365
           S +SL  LA  MQ LQR+EF+  H EW+    P L P +S ++ E  + ++   +   S+
Sbjct: 337 S-SSLKLLANVMQSLQRHEFEHTHSEWMSIVKPDLHPAVSAQLHEKFEVSELEIENSKSV 396

Query: 366 KMELREALAAILEDFGVLAIPTVPGPPPKLNTDVLKLHDFRAKAFSLLSIAGVSGFCQVS 425
           + ELR A+ ++L+D GVL IPTV  PPPKL       HD++++A SLLSIA +SG CQV+
Sbjct: 397 RSELRVAVNSLLKDEGVLVIPTVADPPPKLGGKEFLSHDYQSRALSLLSIASISGCCQVT 456

Query: 426 IPLGLYNGLPVSISLLANHGSDGFLLNVVHSLYNTLEEE 452
           +PLG ++  PVS+SL+A HG D FLL+ + ++Y  L+E+
Sbjct: 457 VPLGFFDKNPVSVSLIARHGGDRFLLDTLKTMYTVLQEQ 457

BLAST of CmUC03G066680 vs. ExPASy Swiss-Prot
Match: F4KCL7 (Outer envelope protein 64, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OM64 PE=1 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 1.0e-97
Identity = 193/465 (41.51%), Postives = 280/465 (60.22%), Query Frame = 0

Query: 6   YGAFMEKFLL----QPSSPSDQIPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANQT 65
           +GAF+++F L     P  P+ +  L+GLTF++ D FD+  ++TGFG P+W +TH  A +T
Sbjct: 47  FGAFLDRFELLPFPPPPPPAAKQSLSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAEKT 106

Query: 66  APAVLTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPHASDRIPGGSSSGSAVAVGT 125
           A  V T+L+ GATC+G+TIMDE+ + I GEN HYGTP NP   D +PGG SSGSAV+VG 
Sbjct: 107 AVVVTTLLKNGATCVGKTIMDELGFGIIGENKHYGTPINPLMPDNVPGGCSSGSAVSVGA 166

Query: 126 ---------DTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGNEWCLIVTLS 185
                    DT G VRVPA++CGILGFRPS G VS+ GV+P +QS +TV           
Sbjct: 167 ELVDFSLGIDTTGGVRVPAAFCGILGFRPSQGTVSSVGVLPNSQSLETV----------- 226

Query: 186 TSFIITDDFVAHDFAYMSDIVLVGNTGWFARDPVILKKVGRLLLEQPEVEYQKPTQVLIA 245
                                     GWFA DP +L +VG  LL    V +++   ++ A
Sbjct: 227 --------------------------GWFASDPSVLCQVGHALLNLSAVTHRRQRSLIFA 286

Query: 246 EDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKHVDLGNYVADKVPSLKHFIIEGNAGHEH 305
           +D F+LS IP ++  Q    +++ L G    KHV++G YVA  VPSL  F  E +   ++
Sbjct: 287 DDLFELSDIPKQKSVQVVRKAIENLSGYKTPKHVNVGQYVASNVPSLAEF-CEQSGKSQN 346

Query: 306 SIASLAALARSMQLLQRYEFKINHEEWVRTHNPHLGPGISDRVSEAMKATDENTDLCHSI 365
           S ++L AL+  M  +QR+EFK NHEEW +T    LGP  S+ V  A+K+ +E+    + +
Sbjct: 347 SASTLRALSSVMLAIQRHEFKTNHEEWWQTCKSFLGPRFSNDVVTALKSKNESIKSLYRV 406

Query: 366 KMELREALAAILEDFGVLAIPTVPGPPPKLNTDVLK-LHDFRAKAFSLLSIAGVSGFCQV 425
           K E+R  + ++L++ G+L IPTV  PPP+LNT   K L++F  + ++L  IA +SG CQV
Sbjct: 407 KNEMRATIQSLLKEDGILVIPTVADPPPRLNTKRNKSLNEFLDRTYALSCIASMSGCCQV 466

Query: 426 SIPLGLYNGLPVSISLLANHGSDGFLLNVVHSLYNTLEEEVKASS 457
           +IPLG +   P+S+SLL  +G D FLL+    +Y +L+++ K +S
Sbjct: 467 TIPLGEHGDRPISVSLLTYYGGDKFLLDTTLDVYASLQDQAKLAS 473

BLAST of CmUC03G066680 vs. ExPASy TrEMBL
Match: A0A1S3BPN7 (amidase 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492180 PE=3 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 2.2e-215
Identity = 387/464 (83.41%), Postives = 404/464 (87.07%), Query Frame = 0

Query: 1   MAVQYYGAFMEKFLLQPSSPSDQIPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANQ 60
           MAVQ YGAFMEKFLLQPSSPSDQ+PLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPAN 
Sbjct: 1   MAVQ-YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60

Query: 61  TAPAVLTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPHASDRIPGGSSSGSAVAV- 120
           TAP V TILRGGATCIGRTIMDEMAYSINGENFHYGTPQNP ASDR+PGGSSSGSAVAV 
Sbjct: 61  TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120

Query: 121 --------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGNEWCLIVTL 180
                   GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTV          
Sbjct: 121 ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTV---------- 180

Query: 181 STSFIITDDFVAHDFAYMSDIVLVGNTGWFARDPVILKKVGRLLLEQPEVEYQKPTQVLI 240
                                      GWFARDPV+ KKVG LLL+QPEVE+QKPTQVLI
Sbjct: 181 ---------------------------GWFARDPVVFKKVGLLLLQQPEVEHQKPTQVLI 240

Query: 241 AEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKHVDLGNYVADKVPSLKHFIIEGNAGHE 300
           AEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIK V+LGNYV +KVPSLK F+IEGNAG+E
Sbjct: 241 AEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLGNYVEEKVPSLKQFMIEGNAGNE 300

Query: 301 HSIASLAALARSMQLLQRYEFKINHEEWVRTHNPHLGPGISDRVSEAMKATDENTDLCHS 360
           HSI SLAALARS+QLLQRYEFKINHEEWVRT+NPHLGPGIS+RVSEAM+ATDEN DLCHS
Sbjct: 301 HSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGPGISERVSEAMRATDENIDLCHS 360

Query: 361 IKMELREALAAILEDFGVLAIPTVPGPPPKLNTDVLKLHDFRAKAFSLLSIAGVSGFCQV 420
           IK+ELR ALAA+LEDFGVLAIPTVPGPPPKLNTDV +LHDFRAKAFSLLSIAGVSGFCQV
Sbjct: 361 IKIELRRALAALLEDFGVLAIPTVPGPPPKLNTDVSELHDFRAKAFSLLSIAGVSGFCQV 420

Query: 421 SIPLGLYNGLPVSISLLANHGSDGFLLNVVHSLYNTLEEEVKAS 456
           SIPLGLYNGLPVSISL+ANHGSDGFLLNVVHSLYNTLEEEVKAS
Sbjct: 421 SIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNTLEEEVKAS 426

BLAST of CmUC03G066680 vs. ExPASy TrEMBL
Match: A0A5D3CG55 (Amidase 1-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003790 PE=4 SV=1)

HSP 1 Score: 756.5 bits (1952), Expect = 6.3e-215
Identity = 387/468 (82.69%), Postives = 404/468 (86.32%), Query Frame = 0

Query: 1   MAVQYYGAFMEKFLLQPSSPSDQIPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANQ 60
           MAVQ YGAFMEKFLLQPSSPSDQ+PLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPAN 
Sbjct: 46  MAVQ-YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 105

Query: 61  TAPAVLTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPHASDRIPGGSSSGSAVA-- 120
           TAP V TILRGGATCIGRTIMDEMAYSINGENFHYGTPQNP ASDR+PGGSSSGSAVA  
Sbjct: 106 TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 165

Query: 121 -----------VGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGNEWCL 180
                      VGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTV      
Sbjct: 166 ANLVDFSLGTFVGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTV------ 225

Query: 181 IVTLSTSFIITDDFVAHDFAYMSDIVLVGNTGWFARDPVILKKVGRLLLEQPEVEYQKPT 240
                                          GWFARDPV+ KKVG LLL+QPEVE+QKPT
Sbjct: 226 -------------------------------GWFARDPVVFKKVGLLLLQQPEVEHQKPT 285

Query: 241 QVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKHVDLGNYVADKVPSLKHFIIEGN 300
           QVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIK V+LGNYV +KVPSLK F+IEGN
Sbjct: 286 QVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLGNYVEEKVPSLKQFMIEGN 345

Query: 301 AGHEHSIASLAALARSMQLLQRYEFKINHEEWVRTHNPHLGPGISDRVSEAMKATDENTD 360
           AG+EHSI SLAALARS+QLLQRYEFKINHEEWVRT+NPHLGPGIS+RVSEAM+ATDEN D
Sbjct: 346 AGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGPGISERVSEAMRATDENID 405

Query: 361 LCHSIKMELREALAAILEDFGVLAIPTVPGPPPKLNTDVLKLHDFRAKAFSLLSIAGVSG 420
           LCHSIK+ELR ALAA+LEDFGVLAIPTVPGPPPKLNTDV +LHDFRAKAFSLLSIAGVSG
Sbjct: 406 LCHSIKIELRRALAALLEDFGVLAIPTVPGPPPKLNTDVSELHDFRAKAFSLLSIAGVSG 465

Query: 421 FCQVSIPLGLYNGLPVSISLLANHGSDGFLLNVVHSLYNTLEEEVKAS 456
           FCQVSIPLGLYNGLPVSISL+ANHGSDGFLLNVVHSLYNTLEEEVKAS
Sbjct: 466 FCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNTLEEEVKAS 475

BLAST of CmUC03G066680 vs. ExPASy TrEMBL
Match: A0A0A0M1K1 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G570110 PE=3 SV=1)

HSP 1 Score: 754.6 bits (1947), Expect = 2.4e-214
Identity = 384/464 (82.76%), Postives = 402/464 (86.64%), Query Frame = 0

Query: 1   MAVQYYGAFMEKFLLQPSSPSDQIPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANQ 60
           MAVQ YGAFMEKFLLQPSSPSDQ+PLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPAN 
Sbjct: 1   MAVQCYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60

Query: 61  TAPAVLTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPHASDRIPGGSSSGSAVAV- 120
           TAP V TILRGGATCIGRTIMDEMAYSINGENFHYGTPQNP ASDR+PGGSSSGSAVAV 
Sbjct: 61  TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120

Query: 121 --------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGNEWCLIVTL 180
                   GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQS DTV          
Sbjct: 121 ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSMDTV---------- 180

Query: 181 STSFIITDDFVAHDFAYMSDIVLVGNTGWFARDPVILKKVGRLLLEQPEVEYQKPTQVLI 240
                                      GWFARDPV+LKKVG LLL+QPEVE+QKP QVLI
Sbjct: 181 ---------------------------GWFARDPVVLKKVGWLLLQQPEVEHQKPRQVLI 240

Query: 241 AEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKHVDLGNYVADKVPSLKHFIIEGNAGHE 300
           AEDCFKLSSIPSERLTQAFVNSVKKLFGGH IK V LGNYV +KVPSLKHF+IEGNAG+E
Sbjct: 241 AEDCFKLSSIPSERLTQAFVNSVKKLFGGHPIKEVSLGNYVEEKVPSLKHFMIEGNAGNE 300

Query: 301 HSIASLAALARSMQLLQRYEFKINHEEWVRTHNPHLGPGISDRVSEAMKATDENTDLCHS 360
           HSI SLAALARS+QLLQRYEFKINHEEWVRT+NPHLGPGIS+RVSEAM+ATDEN DLCHS
Sbjct: 301 HSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGPGISERVSEAMRATDENIDLCHS 360

Query: 361 IKMELREALAAILEDFGVLAIPTVPGPPPKLNTDVLKLHDFRAKAFSLLSIAGVSGFCQV 420
           I++ELR+ALAA+LEDFGVLAIPTVPGPPPKLNTDV +L DFRAKAFSLLSIAGVSGFCQV
Sbjct: 361 IRIELRKALAALLEDFGVLAIPTVPGPPPKLNTDVSELQDFRAKAFSLLSIAGVSGFCQV 420

Query: 421 SIPLGLYNGLPVSISLLANHGSDGFLLNVVHSLYNTLEEEVKAS 456
           SIPLGLYNGLPVSISL+ANHGSDGFLLNVVHSLYNTLEEEVKAS
Sbjct: 421 SIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNTLEEEVKAS 427

BLAST of CmUC03G066680 vs. ExPASy TrEMBL
Match: A0A5A7U6R8 (Amidase 1-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold761G00050 PE=4 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 5.4e-214
Identity = 386/468 (82.48%), Postives = 403/468 (86.11%), Query Frame = 0

Query: 1   MAVQYYGAFMEKFLLQPSSPSDQIPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANQ 60
           MAVQ YGAFMEKFLLQPSSPSDQ+PLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPAN 
Sbjct: 46  MAVQ-YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 105

Query: 61  TAPAVLTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPHASDRIPGGSSSGSAVA-- 120
           TAP V TILRGGATCIGRTIMDEMAYSINGENFHYGTPQNP ASDR+PGGSSSGSAVA  
Sbjct: 106 TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 165

Query: 121 -----------VGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGNEWCL 180
                      VGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTV      
Sbjct: 166 ANLVDFSLGTFVGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTV------ 225

Query: 181 IVTLSTSFIITDDFVAHDFAYMSDIVLVGNTGWFARDPVILKKVGRLLLEQPEVEYQKPT 240
                                          GWFARDPV+ KKVG LLL+QPEVE+QKPT
Sbjct: 226 -------------------------------GWFARDPVVFKKVGLLLLQQPEVEHQKPT 285

Query: 241 QVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKHVDLGNYVADKVPSLKHFIIEGN 300
           QVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIK V+LGNYV +KVPSLK F+IEGN
Sbjct: 286 QVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLGNYVEEKVPSLKQFMIEGN 345

Query: 301 AGHEHSIASLAALARSMQLLQRYEFKINHEEWVRTHNPHLGPGISDRVSEAMKATDENTD 360
           AG+EHSI SLAALARS+QLLQRYEFKINHEEWVRT+NPHLGPGIS+RVSEAM+ATDEN D
Sbjct: 346 AGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGPGISERVSEAMRATDENID 405

Query: 361 LCHSIKMELREALAAILEDFGVLAIPTVPGPPPKLNTDVLKLHDFRAKAFSLLSIAGVSG 420
           LCHSIK+ELR ALAA+LEDFGVLAIPTVPGPPPKLNTDV +LHDFRAKAFSLLSIAGVSG
Sbjct: 406 LCHSIKIELRRALAALLEDFGVLAIPTVPGPPPKLNTDVSELHDFRAKAFSLLSIAGVSG 465

Query: 421 FCQVSIPLGLYNGLPVSISLLANHGSDGFLLNVVHSLYNTLEEEVKAS 456
           FCQVSIPL LYNGLPVSISL+ANHGSDGFLLNVVHSLYNTLEEEVKAS
Sbjct: 466 FCQVSIPLRLYNGLPVSISLVANHGSDGFLLNVVHSLYNTLEEEVKAS 475

BLAST of CmUC03G066680 vs. ExPASy TrEMBL
Match: A0A1S4DYH0 (amidase 1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492180 PE=3 SV=1)

HSP 1 Score: 750.4 bits (1936), Expect = 4.5e-213
Identity = 387/473 (81.82%), Postives = 404/473 (85.41%), Query Frame = 0

Query: 1   MAVQYYGAFMEKFLLQPSSPSDQIPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANQ 60
           MAVQ YGAFMEKFLLQPSSPSDQ+PLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPAN 
Sbjct: 1   MAVQ-YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60

Query: 61  TAPAVLTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPHASDRIPGGSSSGSAVAV- 120
           TAP V TILRGGATCIGRTIMDEMAYSINGENFHYGTPQNP ASDR+PGGSSSGSAVAV 
Sbjct: 61  TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120

Query: 121 --------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGNEWCLIVTL 180
                   GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTV          
Sbjct: 121 ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTV---------- 180

Query: 181 STSFIITDDFVAHDFAYMSDIVLVGNTGWFARDPVILKKVGRLLLEQPEVEYQKPTQVLI 240
                                      GWFARDPV+ KKVG LLL+QPEVE+QKPTQVLI
Sbjct: 181 ---------------------------GWFARDPVVFKKVGLLLLQQPEVEHQKPTQVLI 240

Query: 241 AEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKHVDLGNYVADKVPSLKHFIIEGNAGHE 300
           AEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIK V+LGNYV +KVPSLK F+IEGNAG+E
Sbjct: 241 AEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNLGNYVEEKVPSLKQFMIEGNAGNE 300

Query: 301 HSIASLAALARSMQLLQRYEFKINHEEWVRTHNPHLGPGISDRVSEAMKATDENTDLCHS 360
           HSI SLAALARS+QLLQRYEFKINHEEWVRT+NPHLGPGIS+RVSEAM+ATDEN DLCHS
Sbjct: 301 HSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGPGISERVSEAMRATDENIDLCHS 360

Query: 361 IKMELREALAAILE---------DFGVLAIPTVPGPPPKLNTDVLKLHDFRAKAFSLLSI 420
           IK+ELR ALAA+LE         DFGVLAIPTVPGPPPKLNTDV +LHDFRAKAFSLLSI
Sbjct: 361 IKIELRRALAALLEVLYLSRMLHDFGVLAIPTVPGPPPKLNTDVSELHDFRAKAFSLLSI 420

Query: 421 AGVSGFCQVSIPLGLYNGLPVSISLLANHGSDGFLLNVVHSLYNTLEEEVKAS 456
           AGVSGFCQVSIPLGLYNGLPVSISL+ANHGSDGFLLNVVHSLYNTLEEEVKAS
Sbjct: 421 AGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNTLEEEVKAS 435

BLAST of CmUC03G066680 vs. TAIR 10
Match: AT1G08980.1 (amidase 1 )

HSP 1 Score: 489.2 bits (1258), Expect = 3.6e-138
Identity = 257/452 (56.86%), Postives = 313/452 (69.25%), Query Frame = 0

Query: 6   YGAFMEKFLLQPSSPSDQIP-LTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANQTAPA 65
           +GAF+EK  + P+S S   P L GLTFA+KDIFD++G VTGFGNP+WLRTH  A  TAP 
Sbjct: 7   FGAFIEKVTISPTSTSSSPPSLQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTAPV 66

Query: 66  VLTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPHASDRIPGGSSSGSAVAV----- 125
           V ++L  GAT +G TIMDEMAYSINGEN HYGTP+NP A DR+PGGSSSGSAVAV     
Sbjct: 67  VSSLLEAGATALGITIMDEMAYSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAARLV 126

Query: 126 ----GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGNEWCLIVTLSTSF 185
               GTDTGGSVRVPASYCGI GFRPSHGAVST G+ PMAQSFDTV              
Sbjct: 127 DFSIGTDTGGSVRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTV-------------- 186

Query: 186 IITDDFVAHDFAYMSDIVLVGNTGWFARDPVILKKVGRLLLEQPEVEYQKPTQVLIAEDC 245
                                  GWFARD   LK+VG +LL+Q  +   +P+Q++IA+DC
Sbjct: 187 -----------------------GWFARDTATLKRVGCVLLQQHHLNPIEPSQLIIADDC 246

Query: 246 FKLSSIPSERLTQAFVNSVKKLFGGH-LIKHVDLGNYVADKVPSLKHFIIEGN--AGHEH 305
           FKL S+P + L Q  V SV+K FGG+ ++K V+LG Y+   VPSLKHF+   +     E 
Sbjct: 247 FKLCSVPHDLLVQPLVGSVEKSFGGNTVVKKVNLGEYIGQNVPSLKHFMTSDDVTTQQEF 306

Query: 306 SIASLAALARSMQLLQRYEFKINHEEWVRTHNPHLGPGISDRVSEAMKATDENTDLCHSI 365
            I SL AL+ SM+LLQR+EFKINH  W+ +  P  GPGIS+R+ EA++ +DE  D C S+
Sbjct: 307 CIPSLMALSSSMRLLQRHEFKINHGAWISSVKPEFGPGISERIEEAIRTSDEKIDHCRSV 366

Query: 366 KMELREALAAILEDFGVLAIPTVPGPPPKLNTDVLKLHDFRAKAFSLLSIAGVSGFCQVS 425
           K EL  AL+ +L + GVL IPTVPGPPP L  +V  L  FR++AFSLLSIAGVSGFCQVS
Sbjct: 367 KSELITALSTLLGEKGVLVIPTVPGPPPHLQANVAALESFRSRAFSLLSIAGVSGFCQVS 421

Query: 426 IPLGLYNGLPVSISLLANHGSDGFLLNVVHSL 445
           IPLGL+  LPVS+SL+A +GSDGFLL++V SL
Sbjct: 427 IPLGLHENLPVSVSLVAKYGSDGFLLSLVDSL 421

BLAST of CmUC03G066680 vs. TAIR 10
Match: AT3G17970.1 (translocon at the outer membrane of chloroplasts 64-III )

HSP 1 Score: 397.9 bits (1021), Expect = 1.1e-110
Identity = 202/458 (44.10%), Postives = 288/458 (62.88%), Query Frame = 0

Query: 6   YGAFMEKFLL----QPSSPSDQIPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANQT 65
           +GAF++K +L    QP+ P    PLTGLTFAV D+FD+ G+VTGFG+P+W+RTH  A+ T
Sbjct: 34  FGAFIDKLMLLPPPQPAPPKAPHPLTGLTFAVSDVFDITGYVTGFGHPDWVRTHEAASST 93

Query: 66  APAVLTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPHASDRIPGGSSSGSAVAV-- 125
           +P V T++ GGATC+G+T++DE A+SI+GEN HY +P NP A  RIPGG+ SG+AVAV  
Sbjct: 94  SPVVSTLVEGGATCVGKTVVDEFAFSISGENKHYDSPTNPAAPTRIPGGACSGAAVAVAT 153

Query: 126 -------GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGNEWCLIVTLS 185
                  G DT G VRVPA YCG+LGF+ S+GA+S +G++P++ S D+V           
Sbjct: 154 NAVDFALGIDTVGGVRVPAGYCGVLGFKSSYGAISNTGIIPVSSSLDSV----------- 213

Query: 186 TSFIITDDFVAHDFAYMSDIVLVGNTGWFARDPVILKKVGRLLLEQPEVEYQKPTQVLIA 245
                                     GWFARDP  L++VG +LL+ P    + P Q+++A
Sbjct: 214 --------------------------GWFARDPNTLRRVGHVLLQLPFATQRNPRQIILA 273

Query: 246 EDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKHVDLGNYVADKVPSLKHFIIEGNAGHEH 305
           +DCF+L  IP +R+TQ    S +KLFG  L+KH +L  Y   KVPSLK F     A    
Sbjct: 274 DDCFQLLKIPVDRITQVVTKSAEKLFGRQLLKHQNLETYFETKVPSLKEF-ARTKAIANT 333

Query: 306 SIASLAALARSMQLLQRYEFKINHEEWVRTHNPHLGPGISDRVSEAMKATDENTDLCHSI 365
            +++   LA  MQLLQR+EF  NH +W+ T  P + P I  +V E  + T+E T+  ++I
Sbjct: 334 KVSTSRLLANVMQLLQRHEFLQNHGDWINTVKPAIDPVILSQVCENPELTNEETENLNAI 393

Query: 366 KMELREALAAILEDFGVLAIPTVPGPPPKLNTDVLKLHDFRAKAFSLLSIAGVSGFCQVS 425
           + E R A+ ++L+D G+L IPT+P  PPKL +  +   D++ +A SLLSIA +SG CQV+
Sbjct: 394 RNETRVAIGSLLKDDGILVIPTLPAVPPKLGSKEITSEDYQNRASSLLSIASISGCCQVT 453

Query: 426 IPLGLYNGLPVSISLLANHGSDGFLLNVVHSLYNTLEE 451
           +PLG +   P+S+S +  HG D FLL+ V ++Y +L+E
Sbjct: 454 VPLGHHEKCPISVSFIGRHGGDRFLLDTVQTMYPSLQE 453

BLAST of CmUC03G066680 vs. TAIR 10
Match: AT5G09420.1 (translocon at the outer membrane of chloroplasts 64-V )

HSP 1 Score: 358.6 bits (919), Expect = 7.4e-99
Identity = 193/465 (41.51%), Postives = 280/465 (60.22%), Query Frame = 0

Query: 6   YGAFMEKFLL----QPSSPSDQIPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANQT 65
           +GAF+++F L     P  P+ +  L+GLTF++ D FD+  ++TGFG P+W +TH  A +T
Sbjct: 47  FGAFLDRFELLPFPPPPPPAAKQSLSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAEKT 106

Query: 66  APAVLTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPHASDRIPGGSSSGSAVAVGT 125
           A  V T+L+ GATC+G+TIMDE+ + I GEN HYGTP NP   D +PGG SSGSAV+VG 
Sbjct: 107 AVVVTTLLKNGATCVGKTIMDELGFGIIGENKHYGTPINPLMPDNVPGGCSSGSAVSVGA 166

Query: 126 ---------DTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGNEWCLIVTLS 185
                    DT G VRVPA++CGILGFRPS G VS+ GV+P +QS +TV           
Sbjct: 167 ELVDFSLGIDTTGGVRVPAAFCGILGFRPSQGTVSSVGVLPNSQSLETV----------- 226

Query: 186 TSFIITDDFVAHDFAYMSDIVLVGNTGWFARDPVILKKVGRLLLEQPEVEYQKPTQVLIA 245
                                     GWFA DP +L +VG  LL    V +++   ++ A
Sbjct: 227 --------------------------GWFASDPSVLCQVGHALLNLSAVTHRRQRSLIFA 286

Query: 246 EDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKHVDLGNYVADKVPSLKHFIIEGNAGHEH 305
           +D F+LS IP ++  Q    +++ L G    KHV++G YVA  VPSL  F  E +   ++
Sbjct: 287 DDLFELSDIPKQKSVQVVRKAIENLSGYKTPKHVNVGQYVASNVPSLAEF-CEQSGKSQN 346

Query: 306 SIASLAALARSMQLLQRYEFKINHEEWVRTHNPHLGPGISDRVSEAMKATDENTDLCHSI 365
           S ++L AL+  M  +QR+EFK NHEEW +T    LGP  S+ V  A+K+ +E+    + +
Sbjct: 347 SASTLRALSSVMLAIQRHEFKTNHEEWWQTCKSFLGPRFSNDVVTALKSKNESIKSLYRV 406

Query: 366 KMELREALAAILEDFGVLAIPTVPGPPPKLNTDVLK-LHDFRAKAFSLLSIAGVSGFCQV 425
           K E+R  + ++L++ G+L IPTV  PPP+LNT   K L++F  + ++L  IA +SG CQV
Sbjct: 407 KNEMRATIQSLLKEDGILVIPTVADPPPRLNTKRNKSLNEFLDRTYALSCIASMSGCCQV 466

Query: 426 SIPLGLYNGLPVSISLLANHGSDGFLLNVVHSLYNTLEEEVKASS 457
           +IPLG +   P+S+SLL  +G D FLL+    +Y +L+++ K +S
Sbjct: 467 TIPLGEHGDRPISVSLLTYYGGDKFLLDTTLDVYASLQDQAKLAS 473

BLAST of CmUC03G066680 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 90.1 bits (222), Expect = 4.9e-18
Identity = 56/147 (38.10%), Postives = 74/147 (50.34%), Query Frame = 0

Query: 25  PLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANQTAPAVLTILRGGATCIGRTIMDEM 84
           PL G+   VKD     G  +   +       PP + T  AV  I   G   +G+T MDE 
Sbjct: 107 PLAGVLIGVKDNICTQGMPSTAASRILEHYRPPFDAT--AVKKIKELGGIVVGKTNMDEF 166

Query: 85  AYSINGENFHYGTPQNPHASDRIPGGSSSGSAVAV---------GTDTGGSVRVPASYCG 144
                 E   +    NP    R+PGGSS GSA AV         G+DTGGSVR PAS+CG
Sbjct: 167 GMGSTTEASAFQVTANPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCG 226

Query: 145 ILGFRPSHGAVSTSGVVPMAQSFDTVG 163
           ++G +P++G VS  G++  A S D +G
Sbjct: 227 VVGLKPTYGRVSRFGLMAYASSLDVIG 251

BLAST of CmUC03G066680 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 85.9 bits (211), Expect = 9.2e-17
Identity = 53/161 (32.92%), Postives = 79/161 (49.07%), Query Frame = 0

Query: 26  LTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANQTAPAVLTILRGGATCIGRTIMDEMA 85
           L G+   +KD  D   H T  G   WL       + +  V  +   GA  +G+  M E+ 
Sbjct: 197 LDGIFVTIKDDIDCLPHPTN-GGTTWLHEDRSVEKDSAVVSKLRSCGAILLGKANMHELG 256

Query: 86  YSINGENFHYGTPQNPHASDRIPGGSSSGSA---------VAVGTDTGGSVRVPASYCGI 145
               G N +YGT +NPH   R  GGSSSGSA          A+GTD GGSVR+P++ CGI
Sbjct: 257 MGTTGNNSNYGTTRNPHDPKRYTGGSSSGSAAIVAAGLCSAALGTDGGGSVRIPSALCGI 316

Query: 146 LGFRPSHGAVSTSGVVPMAQSFDTVGNEWCLIVTLSTSFII 178
            G + ++G    +G +    + + +G    L  +L  +F++
Sbjct: 317 TGLKTTYGRTDMTGSLCEGGTVEIIGP---LASSLEDAFLV 353

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038878397.14.5e-21583.01amidase 1-like isoform X1 [Benincasa hispida][more]
XP_008450658.14.5e-21583.41PREDICTED: amidase 1-like isoform X2 [Cucumis melo][more]
TYK10232.11.3e-21482.69amidase 1-like isoform X2 [Cucumis melo var. makuwa][more]
XP_004135624.15.0e-21482.76amidase 1 [Cucumis sativus] >KGN66086.1 hypothetical protein Csa_006860 [Cucumis... [more]
KAA0050880.11.1e-21382.48amidase 1-like isoform X2 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q9FR375.1e-13756.86Amidase 1 OS=Arabidopsis thaliana OX=3702 GN=AMI1 PE=1 SV=1[more]
Q7XTK33.1e-12652.41Amidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMI1 PE=1 SV=1[more]
Q9LVH51.5e-10944.10Outer envelope protein 64, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP6... [more]
Q9MUK53.4e-10944.23Translocon at the outer membrane of chloroplasts 64 OS=Pisum sativum OX=3888 GN=... [more]
F4KCL71.0e-9741.51Outer envelope protein 64, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OM64... [more]
Match NameE-valueIdentityDescription
A0A1S3BPN72.2e-21583.41amidase 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492180 PE=3 SV=1[more]
A0A5D3CG556.3e-21582.69Amidase 1-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A0A0M1K12.4e-21482.76Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G570110 PE=... [more]
A0A5A7U6R85.4e-21482.48Amidase 1-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
A0A1S4DYH04.5e-21381.82amidase 1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492180 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G08980.13.6e-13856.86amidase 1 [more]
AT3G17970.11.1e-11044.10translocon at the outer membrane of chloroplasts 64-III [more]
AT5G09420.17.4e-9941.51translocon at the outer membrane of chloroplasts 64-V [more]
AT3G25660.14.9e-1838.10Amidase family protein [more]
AT5G64440.19.2e-1732.92fatty acid amide hydrolase [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023631Amidase signature domainPFAMPF01425Amidasecoord: 22..162
e-value: 4.7E-38
score: 131.4
coord: 321..438
e-value: 2.2E-6
score: 27.0
IPR036928Amidase signature (AS) superfamilyGENE3D3.90.1300.10Amidase signature (AS) domaincoord: 15..450
e-value: 4.5E-77
score: 262.0
IPR036928Amidase signature (AS) superfamilySUPERFAMILY75304Amidase signature (AS) enzymescoord: 21..446
NoneNo IPR availablePANTHERPTHR46310AMIDASE 1coord: 5..453
NoneNo IPR availablePANTHERPTHR46310:SF7AMIDASE 1coord: 5..453

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmUC03G066680.1CmUC03G066680.1mRNA