CmUC03G063810 (gene) Watermelon (USVL531) v1

Overview
NameCmUC03G063810
Typegene
OrganismCitrullus mucosospermus (Watermelon (USVL531) v1)
DescriptionL-ascorbate oxidase
LocationCmU531Chr03: 27671572 .. 27676446 (+)
RNA-Seq ExpressionCmUC03G063810
SyntenyCmUC03G063810
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTTCCTCCTTTTTACTCATTGCTTCGCTCATTATTCTTCCCTTCCATCTCTTAGCTTAGAGATGGCAAAAGTCGCAAATCAGCCATTGTTTCCTATGCCTTTCCTAGTCATCTTCTTGGTTTTGTCAATCATGTCTGGTTTTGGAATAACACTCTCTGAGGCAGTTCCCAAAGTCAGACACTACAAATGGGAAGTTGAGTACATGTTTTGGTACCCCGACTGTGTCGAAAACATCGTCATGGGAATCAACGGTCAGTTTCCCGGGCCAACGATTAGAGCCAACGTTGGTGACACCATCGTTGTAGAGATGATTAACAAACTCTCCACTGAAGGCCTCGTTATTCACTGGCATGGAATCCTACAACGAGGAACTCCCTGGGCCGATGGCACTGCTTCCATCTCACAGTGTGCCACTAACCCAGGTGAAACCTTCATTTACCGGTTCGTAGTGGATAAGGTCAGAAGGATTTAAAATATCAATGTCAATGAAAGTGCCATATTGTGTCTAATAAAATTTATTCGTTTATTGAACTTTTTTTTTTCGTAGGCTGGGACATATTTTTATCATGGCCATTTAGGGATGCAAAGAGCAGCAGGGTTGTATGGATCCTTGATAGTGGATCTAGAAGAAGGAAAGAAAGAGCCCTTCCATTATGACGAAGATATCAACTTATTGCTTAGTGATTGGTGGCATAAAACTGTTCAAAAGCAAGAAGTTGGCCTCAACTCCAACCCTATGCATTGGATTAATGAGCCCCAGGTAGTTAATTTATGTCTTATCTACTAAATTATGTTTGAGTTAACTCTCAAATATTATATATTTAATTAAATTATGCGATTCAACTCTGAAAAGTTTCATTTTTTATTCTAATATATATTCTTGAAATTTCTTACATATAAACACACATCTTTGAGAGTTTTTTTTTTTTTTTTTTTTTTTGTCCTATTAAAAAAGATGAAGGTGGCATAAAGGTTAAGGTTTAAAATTTGAAAGACCTACTTTAACAAGCAAAAAAAAAAAAAAAGTTATTGAACAATTTCAATATTTTAGGATATTTTTTTTTAAACAAAGTTTAAAATTTATGCATAAAAACAAAACATCTCAAATCAAAAGAGATAATTGAAACATATATGTCTTTACTTAAATTCTAAAGCAAAAACTTTATTTTAAAAATATTTATGAGTGTTCGAACTAGCTTACATGTACCAACTAATTTTACGGAACAATTTATCTGGCCCTAAAATATTTGTGTGTCAAGGAAAACAAATATTTTCAAAATTAATTTTTAAAGAATTTGTATTAGAAAAAAAAAAACTACTTTTTGAATTTTTTTTTTAGTTTTCAAAATTTAAACTCTTTTTTAAAAAATTGCTAGATCGTGAGAAGCTTATAAGTGAAAATAATATTACAAATTTATTTTTTTAAAAAAAATAAATAAAGGTCACATTTGATAATTATTTAATTTAACTTTTTTTTACAATATCAAGTTCATTTCCTCTCAATTTAATATTATAAAAACAAAAACTAGTTTTTTTTACTTTTCAAAACTTTACTTGTTTTTAGAAAATACATGTAAAAGGTATATAAAGAAAAAACAAGAAATTTAGAAGGGAAAGAGAGATCTGTAAAGCTTAATATTCAAAAATTAAAAACAAAAAACTAAGAGCCCGTTTGGCAATCATTTCCTTTCTTGCTTTTTATCTTTTAAAATTAAGCTTATTTCCTCCTAATTTTTTATCATGATTTGCATATTTCTTAAGTACAAGAATTGAATTCTTAGACAAATTCTAAAAATAAAAACAAGCTTTTTAAAAAAAAATACTTTTTTAAAAAATTGGCTTGATTTTTGTAAACATGGGTAAAAAAATTGATTAAAAAAAATAAAAAAGTAAAATAAGTATTTATAAACTTAATTTTTAAAAATTAAGAAAAAAAACTAAATAACTATAAAATACGGCCTAAACAGTTATGTAAATGATTATCACCTTAGATTTAAATTGCTTTCTTTCTTTGGACATGAGAACATATTGACAGATTCCTTTTTTTTTTCAATATTGAAAACGTTTGCTGCTTTTTCCCGAGCAAAAACAAATAAACAAAACAAAAAAAAATATAAAATAAAATAAAAAAATAAACAAAATAAAAAATAAACAAAACAAAAAAACAAAAAAAACAAAAAAAGAAAAAGAAAAACTGTCCACATAATATTGTCTCTCCCCTCTCTAATCTTTCCCCGTTTCTGTCCTTGAATTGTATGTGAGAGAGTTTTATTTTTATTTTATTTTATTTTTTCTCTCTTTTTTTATCCTTTAAACTTCATTAAACCAAACAAAACTAATTACAATCAAAGTTCTTTATTGAAAATAGAGGACACTCAACTAGAATAATGCGGTAGAAGAAAACTTTTTAAAGTAAATTTGTATATAATATAAGAAAATTTAAACTTTCAATTTTATGTAAAATATATTTTTAAATTTTAAAAAATGTCGAATAAGTAAAAAACATATTAAAACATAGAATTAAAAGTTCAACTACTTATTAGAAATTTCTAAAGTTTAAAAATCTATTAGACTCAAACTTTAACAATTCGTGAATAATATTAAGCATAACTGAATTAGAAATATCATCAAACAAGAGTTCAAATGTTCCAATATTTTTTTTCTTACATGTCGGGTGAACTCTAAAAATAAATAAATATGATAGAGAAAAGGAATTTTTTTTTTTTTTTTTAAGCCGGCAGTGTATCCAAAATTTGAATTTTATATTAACTTTTAGGGTTAACTTTTAGCATTAACACTAATGTAGGGAAAATCCATATGGGACACATTTATTGAAATTTAGATAAAATGACATAATTTAAAATTGATTGGAAAAAAAATAATAAAAAAGTTGATTGAAAAAAATTGAGAGATTGGTGGGGCATGAAAATTATCCCAACCCAATCAAAATCCTCAAAGATTATCAAATTTAATCCAATTTATATCCCACAATTAATTAAAATCAACATTAATGATTCATTGATATTTATTTATTAATTAATTAGCCTATATCTTATTGTTGATTTTCCAGGTATAAAAACTACACCTATATGGTCAGCATTAGAAAACCTATATTAGATGGGTTTTAAAGTTATCTAAATAATAATAATAATAACAATAATATAATTGGTTTTAAAAATAAAATCTTATGAAATAAACAAGTATATTTTAATGTTTGTTTGAAATCCTATATCAAAATATGTGAAACAAAATAGACCCTTAGGACAAAAATTCTAAAATATTGGATAAAGAAAATGACTTAAATTGAGACTTGAACATCGTCAAACTGTTCTAAGTTACCAATATACGCATGCAGTCAACTAGTTTCAATTTGCATCCAAAACCTATATGATTTTTTTTCAAAGCTAATTTTGAAACATGTATAAAAAGGTCGAAAATAATATTAGTGTTTGGTCAATTTAGGTAAAGTATTTATTAGAGATTTAATAATTGAACTATAGTGTTGTTGATTAATTAAATAGAATGTTTTTATAATATTTATGTTGTTGTTGTTGTTGTTGAAGGCCATACTGATAAATGGGAGAGGGCAGACCAATTGTTCAATAGCAGCAAAATACAACACAAGTTTGAAGCAATGTGAGTTGAAAGGAGATGAACAATGTGCACCATTTATTCTTCATGTCAAACCCAATAAGACTTATAGGATAAGAATTGCAAGTACCACTTCATTGTCTGCCCTCAACTTTGCCATTCGGGTAACTCTCTCTCTCTCTTAAATGTTAAAATTGCTAAATAGTGATATTTTGTTATATTTATACAAATTTGGTTCTTTTGCTATATTTGATAAGAACGTTGTTATTCTTGATTTTTAATATTTTTTTTAATCATTTTTCTTCTTCAGAATCATCAACTGTTAGTGGTAGAAGCCGACGGTAACTACGTTCAACCATTTCTAACTTCCGACATCGACATTTATTCCGGCGAGTCCTACTCCGTCCTAATCACCACCGATCAACCGGCATCAGAGAATTACTGGGTTTCCGTCGGCGTCCGCGCGCGGCCACCCAAAACGCCTCCGGCACTAACTATCCTCAACTACCTCCCCAACTCCGTCTCCAAATTACCCATTTCTCCGCCCCCGGAAATCCCCAATTGGGACGATTACGACCGCAGCAAAAACTTCACATACAGAATCTTAGCCGCCATGGGTAGCCCAAAGCCACCGGTGAAATCCAACCGCCGTATCTTTCTCCTCAACACCCAAAATCATATAAACGGCGGCTTCGTAAAGTGGGCCATCAATAACGTGTCTTTAGCCCTCCCCCCGACGCCGTACCTCGCCGCCATAAGAATCGGGCTAAACACCACCTTCGACCAAAATCCCCCACCAGAAACATTCCCAGAGGACTATGACATCTACAATCCACCACCGAACACGAACACGACGACCGGAAATGGCGTTTACCGATTCGAATTGTGGGAAATAGTGGATGTAATTCTACAAAATGCCAATACATTGGAGGATAAAAGAAGCGAGATTCACCCTTGGCATTTGCACGGCCATGATTTTTGGGTTTTGGGGTACGGAGAGGGGAAATTTTCCGCCGCCGTAGATGAGAAGAAATTGAATCTGAAAAACCCACCGCTGAGGAACACGGTGGTGATTTTCCCATATGGGTGGACGGCGATTCGATTTGTGGCTGATAATCCAGGAGTTTGGCCTTTCCATTGCCACATTGAACCTCATTTGCATATGGGAATGGGGGTTGTCTTCGCCGAAGGAGTTCATAAGGTCGACAGGATTCCGACGAAGGCTCTGGCTTGTGGTGAAACTGCGAAAGTGCTGATTAATAACCCCACAAAAAAGCCCTAG

mRNA sequence

CTTTTCCTCCTTTTTACTCATTGCTTCGCTCATTATTCTTCCCTTCCATCTCTTAGCTTAGAGATGGCAAAAGTCGCAAATCAGCCATTGTTTCCTATGCCTTTCCTAGTCATCTTCTTGGTTTTGTCAATCATGTCTGGTTTTGGAATAACACTCTCTGAGGCAGTTCCCAAAGTCAGACACTACAAATGGGAAGTTGAGTACATGTTTTGGTACCCCGACTGTGTCGAAAACATCGTCATGGGAATCAACGGTCAGTTTCCCGGGCCAACGATTAGAGCCAACGTTGGTGACACCATCGTTGTAGAGATGATTAACAAACTCTCCACTGAAGGCCTCGTTATTCACTGGCATGGAATCCTACAACGAGGAACTCCCTGGGCCGATGGCACTGCTTCCATCTCACAGTGTGCCACTAACCCAGGTGAAACCTTCATTTACCGGTTCGTAGTGGATAAGGCTGGGACATATTTTTATCATGGCCATTTAGGGATGCAAAGAGCAGCAGGGTTGTATGGATCCTTGATAGTGGATCTAGAAGAAGGAAAGAAAGAGCCCTTCCATTATGACGAAGATATCAACTTATTGCTTAGTGATTGGTGGCATAAAACTGTTCAAAAGCAAGAAGTTGGCCTCAACTCCAACCCTATGCATTGGATTAATGAGCCCCAGGCCATACTGATAAATGGGAGAGGGCAGACCAATTGTTCAATAGCAGCAAAATACAACACAAGTTTGAAGCAATGTGAGTTGAAAGGAGATGAACAATGTGCACCATTTATTCTTCATGTCAAACCCAATAAGACTTATAGGATAAGAATTGCAAGTACCACTTCATTGTCTGCCCTCAACTTTGCCATTCGGAATCATCAACTGTTAGTGGTAGAAGCCGACGGTAACTACGTTCAACCATTTCTAACTTCCGACATCGACATTTATTCCGGCGAGTCCTACTCCGTCCTAATCACCACCGATCAACCGGCATCAGAGAATTACTGGGTTTCCGTCGGCGTCCGCGCGCGGCCACCCAAAACGCCTCCGGCACTAACTATCCTCAACTACCTCCCCAACTCCGTCTCCAAATTACCCATTTCTCCGCCCCCGGAAATCCCCAATTGGGACGATTACGACCGCAGCAAAAACTTCACATACAGAATCTTAGCCGCCATGGGTAGCCCAAAGCCACCGGTGAAATCCAACCGCCGTATCTTTCTCCTCAACACCCAAAATCATATAAACGGCGGCTTCGTAAAGTGGGCCATCAATAACGTGTCTTTAGCCCTCCCCCCGACGCCGTACCTCGCCGCCATAAGAATCGGGCTAAACACCACCTTCGACCAAAATCCCCCACCAGAAACATTCCCAGAGGACTATGACATCTACAATCCACCACCGAACACGAACACGACGACCGGAAATGGCGTTTACCGATTCGAATTGTGGGAAATAGTGGATGTAATTCTACAAAATGCCAATACATTGGAGGATAAAAGAAGCGAGATTCACCCTTGGCATTTGCACGGCCATGATTTTTGGGTTTTGGGGTACGGAGAGGGGAAATTTTCCGCCGCCGTAGATGAGAAGAAATTGAATCTGAAAAACCCACCGCTGAGGAACACGGTGGTGATTTTCCCATATGGGTGGACGGCGATTCGATTTGTGGCTGATAATCCAGGAGTTTGGCCTTTCCATTGCCACATTGAACCTCATTTGCATATGGGAATGGGGGTTGTCTTCGCCGAAGGAGTTCATAAGGTCGACAGGATTCCGACGAAGGCTCTGGCTTGTGGTGAAACTGCGAAAGTGCTGATTAATAACCCCACAAAAAAGCCCTAG

Coding sequence (CDS)

ATGGCAAAAGTCGCAAATCAGCCATTGTTTCCTATGCCTTTCCTAGTCATCTTCTTGGTTTTGTCAATCATGTCTGGTTTTGGAATAACACTCTCTGAGGCAGTTCCCAAAGTCAGACACTACAAATGGGAAGTTGAGTACATGTTTTGGTACCCCGACTGTGTCGAAAACATCGTCATGGGAATCAACGGTCAGTTTCCCGGGCCAACGATTAGAGCCAACGTTGGTGACACCATCGTTGTAGAGATGATTAACAAACTCTCCACTGAAGGCCTCGTTATTCACTGGCATGGAATCCTACAACGAGGAACTCCCTGGGCCGATGGCACTGCTTCCATCTCACAGTGTGCCACTAACCCAGGTGAAACCTTCATTTACCGGTTCGTAGTGGATAAGGCTGGGACATATTTTTATCATGGCCATTTAGGGATGCAAAGAGCAGCAGGGTTGTATGGATCCTTGATAGTGGATCTAGAAGAAGGAAAGAAAGAGCCCTTCCATTATGACGAAGATATCAACTTATTGCTTAGTGATTGGTGGCATAAAACTGTTCAAAAGCAAGAAGTTGGCCTCAACTCCAACCCTATGCATTGGATTAATGAGCCCCAGGCCATACTGATAAATGGGAGAGGGCAGACCAATTGTTCAATAGCAGCAAAATACAACACAAGTTTGAAGCAATGTGAGTTGAAAGGAGATGAACAATGTGCACCATTTATTCTTCATGTCAAACCCAATAAGACTTATAGGATAAGAATTGCAAGTACCACTTCATTGTCTGCCCTCAACTTTGCCATTCGGAATCATCAACTGTTAGTGGTAGAAGCCGACGGTAACTACGTTCAACCATTTCTAACTTCCGACATCGACATTTATTCCGGCGAGTCCTACTCCGTCCTAATCACCACCGATCAACCGGCATCAGAGAATTACTGGGTTTCCGTCGGCGTCCGCGCGCGGCCACCCAAAACGCCTCCGGCACTAACTATCCTCAACTACCTCCCCAACTCCGTCTCCAAATTACCCATTTCTCCGCCCCCGGAAATCCCCAATTGGGACGATTACGACCGCAGCAAAAACTTCACATACAGAATCTTAGCCGCCATGGGTAGCCCAAAGCCACCGGTGAAATCCAACCGCCGTATCTTTCTCCTCAACACCCAAAATCATATAAACGGCGGCTTCGTAAAGTGGGCCATCAATAACGTGTCTTTAGCCCTCCCCCCGACGCCGTACCTCGCCGCCATAAGAATCGGGCTAAACACCACCTTCGACCAAAATCCCCCACCAGAAACATTCCCAGAGGACTATGACATCTACAATCCACCACCGAACACGAACACGACGACCGGAAATGGCGTTTACCGATTCGAATTGTGGGAAATAGTGGATGTAATTCTACAAAATGCCAATACATTGGAGGATAAAAGAAGCGAGATTCACCCTTGGCATTTGCACGGCCATGATTTTTGGGTTTTGGGGTACGGAGAGGGGAAATTTTCCGCCGCCGTAGATGAGAAGAAATTGAATCTGAAAAACCCACCGCTGAGGAACACGGTGGTGATTTTCCCATATGGGTGGACGGCGATTCGATTTGTGGCTGATAATCCAGGAGTTTGGCCTTTCCATTGCCACATTGAACCTCATTTGCATATGGGAATGGGGGTTGTCTTCGCCGAAGGAGTTCATAAGGTCGACAGGATTCCGACGAAGGCTCTGGCTTGTGGTGAAACTGCGAAAGTGCTGATTAATAACCCCACAAAAAAGCCCTAG

Protein sequence

MAKVANQPLFPMPFLVIFLVLSIMSGFGITLSEAVPKVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTIRANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGETFIYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDINLLLSDWWHKTVQKQEVGLNSNPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSKNFTYRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIGLNTTFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSEIHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGETAKVLINNPTKKP
Homology
BLAST of CmUC03G063810 vs. NCBI nr
Match: XP_038880052.1 (L-ascorbate oxidase-like [Benincasa hispida])

HSP 1 Score: 1075.8 bits (2781), Expect = 0.0e+00
Identity = 498/590 (84.41%), Postives = 538/590 (91.19%), Query Frame = 0

Query: 1   MAKVANQPLFPMPFLVIFLVLSIMSGFGITLSEAVPKVRHYKWEVEYMFWYPDCVENIVM 60
           MAK ANQPLFP+PFLVIFL+ S++SGFGITLSEAV   R++KWEVEYMFW PDCVEN+VM
Sbjct: 1   MAKAANQPLFPLPFLVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVM 60

Query: 61  GINGQFPGPTIRANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNP 120
           GINGQFPGPTIRANVGDTI+VE++NKL TEG+VIHWHGILQR TPWADGTASISQCA NP
Sbjct: 61  GINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINP 120

Query: 121 GETFIYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDINLLLSDWW 180
           GETFIY+F  DK GTYFYHGHLGMQR+AGLYGSLI+D EEGKKEPFHYDE+INLLLSDWW
Sbjct: 121 GETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWW 180

Query: 181 HKTVQKQEVGLNSNPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFI 240
           HK+  KQEVGL+S+PM WI EP+ ILINGRGQ +CSIAAKYN +L+QC+LKG EQCAPFI
Sbjct: 181 HKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPFI 240

Query: 241 LHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVL 300
           L VKP KTYRIRIASTTSLSALN AI NHQLLVVEADGNYV+PF+TSDIDIYSGESYSVL
Sbjct: 241 LQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVL 300

Query: 301 ITTDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSKN 360
           ITT+Q  ++NYWVSVGVR R PKTPPALT+LNYLPNSV+KLP+SPPPE PNW+DY RSKN
Sbjct: 301 ITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKN 360

Query: 361 FTYRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIGL 420
           FTYRILAAMGSP PP+K NRRIFLLNTQN +NGGF KWAINNVSL+LP TPYL AI+IGL
Sbjct: 361 FTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGL 420

Query: 421 NTTFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSEI 480
           N TF+QNPPPETF EDYDI+NPPPN NT T NGVY F L E VDVILQNANTL DK SEI
Sbjct: 421 NNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEI 480

Query: 481 HPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW 540
           HPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW
Sbjct: 481 HPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW 540

Query: 541 PFHCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGETAKVLINNPTKKP 591
            FHCHIEPHLHMGMGV+FAEGVHKV  IPTKALACG TAKVLINNPT+KP
Sbjct: 541 AFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKVLINNPTQKP 590

BLAST of CmUC03G063810 vs. NCBI nr
Match: XP_038878978.1 (L-ascorbate oxidase [Benincasa hispida])

HSP 1 Score: 1064.7 bits (2752), Expect = 2.9e-307
Identity = 503/592 (84.97%), Postives = 539/592 (91.05%), Query Frame = 0

Query: 1   MAKVANQPLFPMPFLVIFLVLSIMSGFGITLSEA-VPKVRHYKWEVEYMFWYPDCVENIV 60
           MAKVA++ LFP PFL IF +LSI+ GFGITLSEA  PK+RHYKWEVEYMFW PDCVENIV
Sbjct: 1   MAKVADKSLFPKPFL-IFFILSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIV 60

Query: 61  MGINGQFPGPTIRANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATN 120
           MGI+GQFPGPTIRANVGDT+VVE+ NKL TEG+VIHWHGI+QRGTPWADGTASISQCA N
Sbjct: 61  MGIDGQFPGPTIRANVGDTVVVELTNKLHTEGVVIHWHGIMQRGTPWADGTASISQCAIN 120

Query: 121 PGETFIYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDINLLLSDW 180
           PGETF YRFVVDKAGTYFYHGHLGMQR+AGLYGSLIVD EEGK EPFHYDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPEEGKNEPFHYDEEINLLLSDW 180

Query: 181 WHKTVQKQEVGLNSNPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPF 240
           WH++V KQEVGL+S P+ WI EPQ ILINGRGQ +CSIAAKYN +LKQCELKG EQCAPF
Sbjct: 181 WHQSVHKQEVGLSSKPIRWIGEPQTILINGRGQFDCSIAAKYNNNLKQCELKGGEQCAPF 240

Query: 241 ILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSV 300
           ILHV PNKTYRIRIASTT+L++LNFAI NH+LLVVEADGNY+QPFLTSDIDIYSGESYSV
Sbjct: 241 ILHVLPNKTYRIRIASTTALASLNFAIGNHRLLVVEADGNYIQPFLTSDIDIYSGESYSV 300

Query: 301 LITTDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSK 360
           LITTDQP+SENYWVS+GVRAR PKTPP LTILNYLPNSVSKLPISPPPE P+W+D+DRSK
Sbjct: 301 LITTDQPSSENYWVSIGVRARLPKTPPGLTILNYLPNSVSKLPISPPPETPDWEDFDRSK 360

Query: 361 NFTYRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIG 420
           NFT+RI AAMGSPKPP++ NRRIFLLNTQN +N GFVKWAINNVSLALPPTPYLAA++  
Sbjct: 361 NFTFRIFAAMGSPKPPMRYNRRIFLLNTQNRMN-GFVKWAINNVSLALPPTPYLAAMKFK 420

Query: 421 LNTTFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSE 480
           LNT FDQNPPPETFP DYDI NPP N NT TGNGVYRF + EIVDVILQNAN L D  SE
Sbjct: 421 LNTAFDQNPPPETFPIDYDINNPPQNANTITGNGVYRFNMGEIVDVILQNANMLNDNTSE 480

Query: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGETAKVLI-NNPTKKP 591
           W FHCHIEPHLHMGMGVVFAEGVHKV RIPTKALACG  AK LI NNP + P
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHKVGRIPTKALACGGAAKALINNNPPQDP 590

BLAST of CmUC03G063810 vs. NCBI nr
Match: NP_001284440.1 (L-ascorbate oxidase-like [Cucumis melo] >AAF35910.1 ascorbate oxidase AO1 [Cucumis melo])

HSP 1 Score: 1051.2 bits (2717), Expect = 3.3e-303
Identity = 487/588 (82.82%), Postives = 529/588 (89.97%), Query Frame = 0

Query: 3   KVANQPLFPMPFLVIFLVLSIMSGFGITLSEAVPKVRHYKWEVEYMFWYPDCVENIVMGI 62
           KVANQPLFPMPF  IF + SI  GFGITLS+A  + RH+KWEVEYMF  PDC EN+VMGI
Sbjct: 4   KVANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63

Query: 63  NGQFPGPTIRANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGE 122
           N QFPGPTIRANVGDT+VVE+INKLSTE +VIHWHGILQ+GTPWADGTASISQCA NPGE
Sbjct: 64  NHQFPGPTIRANVGDTVVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123

Query: 123 TFIYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDINLLLSDWWHK 182
           +F Y+FVVDK GTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDE+INLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183

Query: 183 TVQKQEVGLNSNPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFILH 242
           +V KQEVGL+S+PM WI EPQ+ILINGRGQ+NCSI AKY  +++QC+L G E+CAPFIL 
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEECAPFILK 243

Query: 243 VKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLIT 302
           VKPNKTYRIRI STTSLSALNFAI NH+LLVVEADGNYVQPFLTSDIDIYSGESYSVLI 
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303

Query: 303 TDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSKNFT 362
           TDQ  S+NYWVS+GVR R PKTPP LT+LNYLPNS SKLPISPPPE P W+DYDRSKNFT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363

Query: 363 YRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIGLNT 422
           YRILAAMGSPKPPVKS RRIFLLNTQN +NGG+ KWAINNVSL LP TPYL AI+IG+N 
Sbjct: 364 YRILAAMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIND 423

Query: 423 TFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSEIHP 482
           TFDQNPPPET+PE+YDI+ PPPN NT TGNGVYRF+L EIVDV+LQNANTL DK SEIHP
Sbjct: 424 TFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIHP 483

Query: 483 WHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPF 542
           WHLHGHDFWVLGYGEGKFSAAVDE KLNLK+PPLRNTVVIFP+GWT +RFVADNPGVW F
Sbjct: 484 WHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWAF 543

Query: 543 HCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGETAKVLINNPTKKP 591
           HCHIEPHLHMGMGVVFAEGV KV RIPT A+ACG TAK LI NPT+KP
Sbjct: 544 HCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTAKALIQNPTQKP 591

BLAST of CmUC03G063810 vs. NCBI nr
Match: TYK09892.1 (L-ascorbate oxidase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1047.3 bits (2707), Expect = 4.8e-302
Identity = 487/588 (82.82%), Postives = 527/588 (89.63%), Query Frame = 0

Query: 3   KVANQPLFPMPFLVIFLVLSIMSGFGITLSEAVPKVRHYKWEVEYMFWYPDCVENIVMGI 62
           K ANQPLFPMPF  IF + SI  GFGITLS+A  + RH+KWEVEYMF  PDC EN+VMGI
Sbjct: 4   KDANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63

Query: 63  NGQFPGPTIRANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGE 122
           N QFPGPTIRANVGDTIVVE+INKLSTE +VIHWHGILQ+GTPWADGTASISQCA NPGE
Sbjct: 64  NHQFPGPTIRANVGDTIVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123

Query: 123 TFIYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDINLLLSDWWHK 182
           +F Y+FVVDK GTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDE+INLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183

Query: 183 TVQKQEVGLNSNPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFILH 242
           +V KQEVGL+S+PM WI EPQ+ILINGRGQ+NCSI AKY  +++QC+L G E CAPFIL 
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEGCAPFILK 243

Query: 243 VKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLIT 302
           VKPNKTYRIRI STTSLSALNFAI NH+LLVVEADGNYVQPFLTSDIDIYSGESYSVLI 
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303

Query: 303 TDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSKNFT 362
           TDQ  S+NYWVS+GVR R PKTPP LT+LNYLPNS SKLPISPPPE P W+DYDRSKNFT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363

Query: 363 YRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIGLNT 422
           YRILAAMGSPKPPVKS RRIFLLNTQN +NGG+ KWAINNVSL LP TPYL AI+IG+N 
Sbjct: 364 YRILAAMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIND 423

Query: 423 TFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSEIHP 482
           TFDQNPPPET+PE+YDI+ PPPN NT TGNGVYRF+L EIVDV+LQNANTL DK SEIHP
Sbjct: 424 TFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIHP 483

Query: 483 WHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPF 542
           WHLHGHDFWVLGYGEGKFSAAVDE KLNLK+PPLRNTVVIFP+GWT +RFVADNPGVW F
Sbjct: 484 WHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWAF 543

Query: 543 HCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGETAKVLINNPTKKP 591
           HCHIEPHLHMGMGVVFAEGV KV RIPT A+ACG TAK LI NPT+KP
Sbjct: 544 HCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTAKALIQNPTQKP 591

BLAST of CmUC03G063810 vs. NCBI nr
Match: KAA0055636.1 (L-ascorbate oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 1020.8 bits (2638), Expect = 4.8e-294
Identity = 482/591 (81.56%), Postives = 521/591 (88.16%), Query Frame = 0

Query: 1   MAKVANQPLFPMPFLVIFLVLSIMSGFGITLSEA-VPKVRHYKWEVEYMFWYPDCVENIV 60
           MAKVA++P FP PFLV F VLSI+ GFGITLSEA  PK+RHYKWEVEYMFW PDCVENIV
Sbjct: 1   MAKVADKPFFPKPFLV-FFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIV 60

Query: 61  MGINGQFPGPTIRANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATN 120
           MGINGQFPGPTIRAN GD +VVE+ NKL TEG+VIHWHGILQRGTPWADGTASISQCA N
Sbjct: 61  MGINGQFPGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120

Query: 121 PGETFIYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDINLLLSDW 180
           PGETF YRFVVDKAGTYFYHGHLGMQR+AGLYGSLIVD +EG  EPFHYDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDW 180

Query: 181 WHKTVQKQEVGLNSNPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPF 240
           WH++V KQEVGL+S PM WI EPQ+ILINGRGQ +CSIAAKY   LKQCEL G EQCAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPF 240

Query: 241 ILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSV 300
           ILHV P KTYRIRIASTT+L++LNFAI NH+LLVVEADGNYVQPF TSDIDIYSGESYSV
Sbjct: 241 ILHVHPKKTYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSV 300

Query: 301 LITTDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSK 360
           LITTDQ  SENYWVS+GVRAR PKTPP LT+LNYLPNSVSKLPISPPPE P+W+D+DRSK
Sbjct: 301 LITTDQNPSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSK 360

Query: 361 NFTYRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIG 420
           NFT+RI AAMGSPKPPV+ NRR+FLLNTQN IN GF+KWAINNVSLALPPTPYLAA++  
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKFR 420

Query: 421 LNTTFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSE 480
           L+T F+QNPPPETFP DYDI NPP N  T TGNGVY+F + E VDVILQNAN L    SE
Sbjct: 421 LSTAFNQNPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSE 480

Query: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLGYGEGKFSAA D +KLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGETAKVLINNPTKKP 591
           W FHCHIEPHLHMGMGV+FAEGVHKV  IP  A+ACG TA  +I N  +KP
Sbjct: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGMIPPNAMACGSTA--MIKNYPRKP 587

BLAST of CmUC03G063810 vs. ExPASy Swiss-Prot
Match: P14133 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 1018.1 bits (2631), Expect = 4.1e-296
Identity = 479/591 (81.05%), Postives = 523/591 (88.49%), Query Frame = 0

Query: 1   MAKVANQPLFPMPFLVIFLVLSIMSGFGITLSEA-VPKVRHYKWEVEYMFWYPDCVENIV 60
           MAKVA++P FP PFL  FLVLSI+ GFGITLSEA  PK++HYKW+VEYMFW PDCVENIV
Sbjct: 1   MAKVADKPFFPKPFL-SFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIV 60

Query: 61  MGINGQFPGPTIRANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATN 120
           MGING+FPGPTIRAN GD +VVE+ NKL TEG+VIHWHGILQRGTPWADGTASISQCA N
Sbjct: 61  MGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120

Query: 121 PGETFIYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDINLLLSDW 180
           PGETF YRFVVDKAGTYFYHGHLGMQR+AGLYGSLIVD  EG+ EPFHYDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDW 180

Query: 181 WHKTVQKQEVGLNSNPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPF 240
           WH++V KQEVGL+S PM WI EPQ+ILING+GQ +CSIAAKYN  LKQCEL G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPF 240

Query: 241 ILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSV 300
           ILHV+P KTYRIRIASTT+L++LNFAI NH+LLVVEADGNYVQPF+TSDIDIYSGESYSV
Sbjct: 241 ILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSV 300

Query: 301 LITTDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSK 360
           LITTDQ   ENYWVS+GVRAR PKTPP LT+LNYLPNS SKLPISPPPE P+W+D+DRSK
Sbjct: 301 LITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSK 360

Query: 361 NFTYRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIG 420
           NFT+RI AAMGSPKPPV+ NRR+FLLNTQN IN GF+KWAINNVSLALPPTPYLAA+++ 
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKMR 420

Query: 421 LNTTFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSE 480
           LNT F+QNPPPETFP +YDI NPPPN  TTTGNGVY+F + E VDVILQNAN L    SE
Sbjct: 421 LNTAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSE 480

Query: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLGYGEGKF A  DEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGETAKVLINNPTKKP 591
           W FHCHIEPHLHMGMGVVFAEGVH V  IP KALACG TA  L+ N  + P
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTA--LVKNYPRLP 587

BLAST of CmUC03G063810 vs. ExPASy Swiss-Prot
Match: P24792 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)

HSP 1 Score: 958.7 bits (2477), Expect = 2.9e-278
Identity = 450/573 (78.53%), Postives = 493/573 (86.04%), Query Frame = 0

Query: 14  FLVIFLVLSIMSGFGITLSEAVPKVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTIRA 73
           FL++F    ++  FGI  S    ++RHYKWEVEYMFW PDC ENIVMGINGQFPGPTIRA
Sbjct: 12  FLILFF-FGLILAFGI--SSEGSQIRHYKWEVEYMFWAPDCNENIVMGINGQFPGPTIRA 71

Query: 74  NVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGETFIYRFVVDKA 133
           N GDT+VVE+INKL TEG+VIHWHGILQRGTPWADGTASISQCA NPGETF Y F VD  
Sbjct: 72  NAGDTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNP 131

Query: 134 GTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDINLLLSDWWHKTVQKQEVGLNS 193
           GT+FYHGHLGMQR+AGLYGSLIVD  +GKKEPFHYD +INLLLSDWWH+++ KQEVGL+S
Sbjct: 132 GTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSS 191

Query: 194 NPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFILHVKPNKTYRIRI 253
            P+ WI EPQ IL+NGRGQ +CSIAAKY+++L+ C+LKG E CAP+I HV P KTYRIRI
Sbjct: 192 KPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHVMPKKTYRIRI 251

Query: 254 ASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENYWV 313
           ASTT+L+ALNFAI NH LLVVEADGNYVQPF TSDIDIYSGESYSVLITTDQ  SENYWV
Sbjct: 252 ASTTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWV 311

Query: 314 SVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSKNFTYRILAAMGSPK 373
           SVG R R P TPP LT+LNYLPNSVSKLP SPPPE P WDD+DRSKNFTYRI AAMGSPK
Sbjct: 312 SVGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRITAAMGSPK 371

Query: 374 PPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIGLNTTFDQNPPPETF 433
           PPVKSNRRIFLLNTQN IN G+VKWAIN+VSLALPPTPYL A++  L   FDQNPPPE F
Sbjct: 372 PPVKSNRRIFLLNTQNVIN-GYVKWAINDVSLALPPTPYLGAMKFNLLHAFDQNPPPEVF 431

Query: 434 PEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSEIHPWHLHGHDFWVL 493
           PEDYDI  PP N  T  GNGVY+F++ EIVDVILQNAN +++  SEIHPWHLHGHDFWVL
Sbjct: 432 PEDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLHGHDFWVL 491

Query: 494 GYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMG 553
           GYG+GKF+A  +E  LNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW FHCHIEPHLHMG
Sbjct: 492 GYGDGKFTAE-EESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMG 551

Query: 554 MGVVFAEGVHKVDRIPTKALACGETAKVLINNP 587
           MGVVFAEGV KV RIPTKALACG TAK LINNP
Sbjct: 552 MGVVFAEGVEKVGRIPTKALACGGTAKSLINNP 579

BLAST of CmUC03G063810 vs. ExPASy Swiss-Prot
Match: P37064 (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)

HSP 1 Score: 949.5 bits (2453), Expect = 1.8e-275
Identity = 439/550 (79.82%), Postives = 481/550 (87.45%), Query Frame = 0

Query: 37  KVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTIRANVGDTIVVEMINKLSTEGLVIHW 96
           ++RHYKWEVEYMFW P+C ENIVMGINGQFPGPTIRAN GD++VVE+ NKL TEG+VIHW
Sbjct: 2   QIRHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIHW 61

Query: 97  HGILQRGTPWADGTASISQCATNPGETFIYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIV 156
           HGILQRGTPWADGTASISQCA NPGETF Y F VD  GT+FYHGHLGMQR+AGLYGSLIV
Sbjct: 62  HGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIV 121

Query: 157 DLEEGKKEPFHYDEDINLLLSDWWHKTVQKQEVGLNSNPMHWINEPQAILINGRGQTNCS 216
           D  +GKKEPFHYD +INLLLSDWWH+++ KQEVGL+S P+ WI EPQ IL+NGRGQ +CS
Sbjct: 122 DPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSSKPIRWIGEPQTILLNGRGQFDCS 181

Query: 217 IAAKYNTSLKQCELKGDEQCAPFILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEA 276
           IAAKY+++L+ C+LKG E CAP+I HV P KTYRIRIASTT+L+ALNFAI NHQLLVVEA
Sbjct: 182 IAAKYDSNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLVVEA 241

Query: 277 DGNYVQPFLTSDIDIYSGESYSVLITTDQPASENYWVSVGVRARPPKTPPALTILNYLPN 336
           DGNYVQPF TSDIDIYSGESYSVLITTDQ  SENYWVSVG RAR P TPP LT+LNYLPN
Sbjct: 242 DGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNYLPN 301

Query: 337 SVSKLPISPPPEIPNWDDYDRSKNFTYRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFV 396
           SVSKLP SPPP+ P WDD+DRSKNFTYRI AAMGSPKPPVK NRRIFLLNTQN IN G+V
Sbjct: 302 SVSKLPTSPPPQTPAWDDFDRSKNFTYRITAAMGSPKPPVKFNRRIFLLNTQNVIN-GYV 361

Query: 397 KWAINNVSLALPPTPYLAAIRIGLNTTFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYR 456
           KWAIN+VSLALPPTPYL A++  L   FDQNPPPE FPEDYDI  PP N  T  GNGVY+
Sbjct: 362 KWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFPEDYDIDTPPTNEKTRIGNGVYQ 421

Query: 457 FELWEIVDVILQNANTLEDKRSEIHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPL 516
           F++ E+VDVILQNAN +++  SE HPWHLHGHDFWVLGYG+GKFSA  +E  LNLKNPPL
Sbjct: 422 FKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSAE-EESSLNLKNPPL 481

Query: 517 RNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACG 576
           RNTVVIFPYGWTAIRFVADNPGVW FHCHIEPHLHMGMGVVFAEGV KV RIPTKALACG
Sbjct: 482 RNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKALACG 541

Query: 577 ETAKVLINNP 587
            TAK LINNP
Sbjct: 542 GTAKSLINNP 549

BLAST of CmUC03G063810 vs. ExPASy Swiss-Prot
Match: Q40588 (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 1.2e-236
Identity = 379/573 (66.14%), Postives = 451/573 (78.71%), Query Frame = 0

Query: 14  FLVIFLVLSIMSGFGITLSEAVPKVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTIRA 73
           FL  FL+  I+     + S    K RH+KW+VEY+ W PD  E++VMGINGQFPGPTIRA
Sbjct: 6   FLFFFLLPLILLELSSSRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPTIRA 65

Query: 74  NVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGETFIYRFVVDKA 133
             GDT+ V + NKL TEG+VIHWHGI Q GTPWADGTA+ISQCA NPGETF+YRF VDKA
Sbjct: 66  KAGDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKVDKA 125

Query: 134 GTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDINLLLSDWWHKTVQKQEVGLNS 193
           GTYFYHGH GMQR+AGLYGSLIV++ EG+KEPFHYD + NLLLSDWWHK   +QEV L+S
Sbjct: 126 GTYFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDLSS 185

Query: 194 NPMHWINEPQAILINGRGQTNCSIAAKYN-TSLKQCELKGDEQCAPFILHVKPNKTYRIR 253
           NP+ WI EPQ +L+NGRGQ NCS+AA+++   L QC+L+G EQ AP IL V+PNK YR+R
Sbjct: 186 NPLRWIGEPQTLLLNGRGQYNCSLAARFSKPPLPQCKLRGGEQYAPQILRVRPNKIYRLR 245

Query: 254 IASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENYW 313
           +ASTT+L +L+ AI  H+++VVEADGNYVQPF   D+DIYSGESYSVL  TDQ  ++NYW
Sbjct: 246 VASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTKNYW 305

Query: 314 VSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSKNFTYRILAAMGSP 373
           +S+ VR R PKTP  LT+LNYLPNS SK P  PPP  P W+DY+ SK+F+ +I A MGSP
Sbjct: 306 ISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKIFALMGSP 365

Query: 374 KPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIGLNTTFDQNPPPET 433
           KPP +++RRI LLNTQN I+ G+ KWAINNVSL LP   YL +IR G+N  FD  PPP+ 
Sbjct: 366 KPPPQNHRRIILLNTQNKID-GYTKWAINNVSLVLPTQLYLGSIRYGIN-AFDTKPPPDN 425

Query: 434 FPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSEIHPWHLHGHDFWV 493
           FP+DYD+    PN+N+T GNGVY  +    +D+ILQNAN L    SEIHPWHLHGHDFWV
Sbjct: 426 FPKDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLHGHDFWV 485

Query: 494 LGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHM 553
           LGYGEGKFS   D KK NLKNPPLRNT VIFP+GWTA+RFV DNPGVW FHCHIEPHLHM
Sbjct: 486 LGYGEGKFSEK-DVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHCHIEPHLHM 545

Query: 554 GMGVVFAEGVHKVDRIPTKALACGETAKVLINN 586
           GMGV+FAEGVH V +IP +ALACG T K+L++N
Sbjct: 546 GMGVIFAEGVHLVKKIPKEALACGLTGKMLMSN 575

BLAST of CmUC03G063810 vs. ExPASy Swiss-Prot
Match: M4DUF2 (L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 4.8e-228
Identity = 360/548 (65.69%), Postives = 431/548 (78.65%), Query Frame = 0

Query: 38  VRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTIRANVGDTIVVEMINKLSTEGLVIHWH 97
           VR Y WEVEY F +PDC E +VM +NGQFPGPTI A  GDTIVV + NKL+TEGLVIHWH
Sbjct: 20  VREYAWEVEYKFGWPDCKEGMVMAVNGQFPGPTIHALAGDTIVVHLTNKLATEGLVIHWH 79

Query: 98  GILQRGTPWADGTASISQCATNPGETFIYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVD 157
           GI Q G+PWADG A ++QCA +PGETF Y F VDK GT+FYHGH GMQR+AGLYGSLI+D
Sbjct: 80  GIRQLGSPWADGAAGVTQCAISPGETFTYNFTVDKPGTHFYHGHYGMQRSAGLYGSLIID 139

Query: 158 LEEGKKEPFHYDEDINLLLSDWWHKTVQKQEVGLNSNPMHWINEPQAILINGRGQTNCSI 217
           + +GKKEP  YD + NLLLSDWWH+ V  QE+GL+S PM WI E Q+ILINGRGQ NCS+
Sbjct: 140 VAKGKKEPLRYDGEFNLLLSDWWHEDVLSQEIGLSSRPMRWIGEAQSILINGRGQFNCSL 199

Query: 218 AAKY-NTSLKQCELKGDEQCAPFILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEA 277
           AA++ +TSL  C  K  +QCAP  LHV+PNKTYRIR+AS+T+L++LNFA++ H+L+VVEA
Sbjct: 200 AAQFSSTSLPTCTFKEGDQCAPQRLHVEPNKTYRIRLASSTALASLNFAVQGHKLVVVEA 259

Query: 278 DGNYVQPFLTSDIDIYSGESYSVLITTDQPASENYWVSVGVRARPPKTPPALTILNYLPN 337
           DGNY+ PF T DIDIYSGE+YSVL+TTDQ  S+NY+++ GVR R P TPPALT+LNY+  
Sbjct: 260 DGNYITPFTTDDIDIYSGETYSVLLTTDQDPSQNYYITAGVRGRKPNTPPALTVLNYVTA 319

Query: 338 SVSKLPISPPPEIPNWDDYDRSKNFTYRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFV 397
             S+LP SPPPE P W+D+DRSKNF+ +I AAMGSP PP   + R+ LLNTQN I  GF 
Sbjct: 320 PSSQLPTSPPPETPRWNDFDRSKNFSKKIFAAMGSPSPPETFDERLILLNTQNLIE-GFT 379

Query: 398 KWAINNVSLALPPTPYLAAIRIGLNTTFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYR 457
           KWAINNVSLA+P TPYL +++  L T F+++ PP+ +P DYDI  PP N N   GN    
Sbjct: 380 KWAINNVSLAVPGTPYLGSVKYNLRTGFNRSSPPKDYPVDYDIMTPPRNRNAKQGNVSCV 439

Query: 458 FELWEIVDVILQNANTLEDKRSEIHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPL 517
           F     VDVILQNAN L    SEIHPWHLHGHDFWVLGYGEGKF   VDEK  NLKNPPL
Sbjct: 440 FPFNVTVDVILQNANGLNANASEIHPWHLHGHDFWVLGYGEGKFKPGVDEKTYNLKNPPL 499

Query: 518 RNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACG 577
           RNTV ++PYGWTA+RFV DNPGVW FHCHIEPHLHMGMGVVFAEG++++ ++P +AL CG
Sbjct: 500 RNTVALYPYGWTALRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGKVPDEALGCG 559

Query: 578 ETAKVLIN 585
            T + L+N
Sbjct: 560 LTKQFLMN 566

BLAST of CmUC03G063810 vs. ExPASy TrEMBL
Match: Q9M5B5 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=2C07 PE=2 SV=1)

HSP 1 Score: 1051.2 bits (2717), Expect = 1.6e-303
Identity = 487/588 (82.82%), Postives = 529/588 (89.97%), Query Frame = 0

Query: 3   KVANQPLFPMPFLVIFLVLSIMSGFGITLSEAVPKVRHYKWEVEYMFWYPDCVENIVMGI 62
           KVANQPLFPMPF  IF + SI  GFGITLS+A  + RH+KWEVEYMF  PDC EN+VMGI
Sbjct: 4   KVANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63

Query: 63  NGQFPGPTIRANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGE 122
           N QFPGPTIRANVGDT+VVE+INKLSTE +VIHWHGILQ+GTPWADGTASISQCA NPGE
Sbjct: 64  NHQFPGPTIRANVGDTVVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123

Query: 123 TFIYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDINLLLSDWWHK 182
           +F Y+FVVDK GTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDE+INLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183

Query: 183 TVQKQEVGLNSNPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFILH 242
           +V KQEVGL+S+PM WI EPQ+ILINGRGQ+NCSI AKY  +++QC+L G E+CAPFIL 
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEECAPFILK 243

Query: 243 VKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLIT 302
           VKPNKTYRIRI STTSLSALNFAI NH+LLVVEADGNYVQPFLTSDIDIYSGESYSVLI 
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303

Query: 303 TDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSKNFT 362
           TDQ  S+NYWVS+GVR R PKTPP LT+LNYLPNS SKLPISPPPE P W+DYDRSKNFT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363

Query: 363 YRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIGLNT 422
           YRILAAMGSPKPPVKS RRIFLLNTQN +NGG+ KWAINNVSL LP TPYL AI+IG+N 
Sbjct: 364 YRILAAMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIND 423

Query: 423 TFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSEIHP 482
           TFDQNPPPET+PE+YDI+ PPPN NT TGNGVYRF+L EIVDV+LQNANTL DK SEIHP
Sbjct: 424 TFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIHP 483

Query: 483 WHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPF 542
           WHLHGHDFWVLGYGEGKFSAAVDE KLNLK+PPLRNTVVIFP+GWT +RFVADNPGVW F
Sbjct: 484 WHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWAF 543

Query: 543 HCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGETAKVLINNPTKKP 591
           HCHIEPHLHMGMGVVFAEGV KV RIPT A+ACG TAK LI NPT+KP
Sbjct: 544 HCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTAKALIQNPTQKP 591

BLAST of CmUC03G063810 vs. ExPASy TrEMBL
Match: A0A5D3CF11 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G00020 PE=3 SV=1)

HSP 1 Score: 1047.3 bits (2707), Expect = 2.3e-302
Identity = 487/588 (82.82%), Postives = 527/588 (89.63%), Query Frame = 0

Query: 3   KVANQPLFPMPFLVIFLVLSIMSGFGITLSEAVPKVRHYKWEVEYMFWYPDCVENIVMGI 62
           K ANQPLFPMPF  IF + SI  GFGITLS+A  + RH+KWEVEYMF  PDC EN+VMGI
Sbjct: 4   KDANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63

Query: 63  NGQFPGPTIRANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGE 122
           N QFPGPTIRANVGDTIVVE+INKLSTE +VIHWHGILQ+GTPWADGTASISQCA NPGE
Sbjct: 64  NHQFPGPTIRANVGDTIVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123

Query: 123 TFIYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDINLLLSDWWHK 182
           +F Y+FVVDK GTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDE+INLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183

Query: 183 TVQKQEVGLNSNPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPFILH 242
           +V KQEVGL+S+PM WI EPQ+ILINGRGQ+NCSI AKY  +++QC+L G E CAPFIL 
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEGCAPFILK 243

Query: 243 VKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLIT 302
           VKPNKTYRIRI STTSLSALNFAI NH+LLVVEADGNYVQPFLTSDIDIYSGESYSVLI 
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303

Query: 303 TDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSKNFT 362
           TDQ  S+NYWVS+GVR R PKTPP LT+LNYLPNS SKLPISPPPE P W+DYDRSKNFT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363

Query: 363 YRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIGLNT 422
           YRILAAMGSPKPPVKS RRIFLLNTQN +NGG+ KWAINNVSL LP TPYL AI+IG+N 
Sbjct: 364 YRILAAMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIND 423

Query: 423 TFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSEIHP 482
           TFDQNPPPET+PE+YDI+ PPPN NT TGNGVYRF+L EIVDV+LQNANTL DK SEIHP
Sbjct: 424 TFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIHP 483

Query: 483 WHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPF 542
           WHLHGHDFWVLGYGEGKFSAAVDE KLNLK+PPLRNTVVIFP+GWT +RFVADNPGVW F
Sbjct: 484 WHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWAF 543

Query: 543 HCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGETAKVLINNPTKKP 591
           HCHIEPHLHMGMGVVFAEGV KV RIPT A+ACG TAK LI NPT+KP
Sbjct: 544 HCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTAKALIQNPTQKP 591

BLAST of CmUC03G063810 vs. ExPASy TrEMBL
Match: A0A5A7UMY3 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G00010 PE=3 SV=1)

HSP 1 Score: 1020.8 bits (2638), Expect = 2.3e-294
Identity = 482/591 (81.56%), Postives = 521/591 (88.16%), Query Frame = 0

Query: 1   MAKVANQPLFPMPFLVIFLVLSIMSGFGITLSEA-VPKVRHYKWEVEYMFWYPDCVENIV 60
           MAKVA++P FP PFLV F VLSI+ GFGITLSEA  PK+RHYKWEVEYMFW PDCVENIV
Sbjct: 1   MAKVADKPFFPKPFLV-FFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIV 60

Query: 61  MGINGQFPGPTIRANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATN 120
           MGINGQFPGPTIRAN GD +VVE+ NKL TEG+VIHWHGILQRGTPWADGTASISQCA N
Sbjct: 61  MGINGQFPGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120

Query: 121 PGETFIYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDINLLLSDW 180
           PGETF YRFVVDKAGTYFYHGHLGMQR+AGLYGSLIVD +EG  EPFHYDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDW 180

Query: 181 WHKTVQKQEVGLNSNPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPF 240
           WH++V KQEVGL+S PM WI EPQ+ILINGRGQ +CSIAAKY   LKQCEL G EQCAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPF 240

Query: 241 ILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSV 300
           ILHV P KTYRIRIASTT+L++LNFAI NH+LLVVEADGNYVQPF TSDIDIYSGESYSV
Sbjct: 241 ILHVHPKKTYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSV 300

Query: 301 LITTDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSK 360
           LITTDQ  SENYWVS+GVRAR PKTPP LT+LNYLPNSVSKLPISPPPE P+W+D+DRSK
Sbjct: 301 LITTDQNPSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSK 360

Query: 361 NFTYRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIG 420
           NFT+RI AAMGSPKPPV+ NRR+FLLNTQN IN GF+KWAINNVSLALPPTPYLAA++  
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKFR 420

Query: 421 LNTTFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSE 480
           L+T F+QNPPPETFP DYDI NPP N  T TGNGVY+F + E VDVILQNAN L    SE
Sbjct: 421 LSTAFNQNPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSE 480

Query: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLGYGEGKFSAA D +KLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGETAKVLINNPTKKP 591
           W FHCHIEPHLHMGMGV+FAEGVHKV  IP  A+ACG TA  +I N  +KP
Sbjct: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGMIPPNAMACGSTA--MIKNYPRKP 587

BLAST of CmUC03G063810 vs. ExPASy TrEMBL
Match: Q9M5B4 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492479 PE=2 SV=1)

HSP 1 Score: 1018.5 bits (2632), Expect = 1.2e-293
Identity = 481/591 (81.39%), Postives = 520/591 (87.99%), Query Frame = 0

Query: 1   MAKVANQPLFPMPFLVIFLVLSIMSGFGITLSEA-VPKVRHYKWEVEYMFWYPDCVENIV 60
           MAKVA++P FP PFLV F VLSI+ GFGITLSEA  PK+RHYKWEVEYMFW PDCVENIV
Sbjct: 1   MAKVADKPFFPKPFLV-FFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIV 60

Query: 61  MGINGQFPGPTIRANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATN 120
           MGINGQFPGPTIRAN GD +VVE+ NKL TEG+VIHWHGILQRGTPWADGTASISQCA N
Sbjct: 61  MGINGQFPGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120

Query: 121 PGETFIYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDINLLLSDW 180
           PGETF YRFVVDKAGTYFYHGHLGMQR+AGLYGSLIVD +EG  EPFHYDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDW 180

Query: 181 WHKTVQKQEVGLNSNPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPF 240
           WH++V KQEVGL+S PM WI EPQ+ILINGRGQ +CSIAAKY   LKQCEL G EQCAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPF 240

Query: 241 ILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSV 300
           ILHV P K YRIRIASTT+L++LNFAI NH+LLVVEADGNYVQPF TSDIDIYSGESYSV
Sbjct: 241 ILHVHPKKIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSV 300

Query: 301 LITTDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSK 360
           LITTDQ  SENYWVS+GVRAR PKTPP LT+LNYLPNSVSKLPISPPPE P+W+D+DRSK
Sbjct: 301 LITTDQNPSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSK 360

Query: 361 NFTYRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIG 420
           NFT+RI AAMGSPKPPV+ NRR+FLLNTQN IN GF+KWAINNVSLALPPTPYLAA++  
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKFR 420

Query: 421 LNTTFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSE 480
           L+T F+QNPPPETFP DYDI NPP N  T TGNGVY+F + E VDVILQNAN L    SE
Sbjct: 421 LSTAFNQNPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSE 480

Query: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLGYGEGKFSAA D +KLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGETAKVLINNPTKKP 591
           W FHCHIEPHLHMGMGV+FAEGVHKV  IP  A+ACG TA  +I N  +KP
Sbjct: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGMIPPNAMACGSTA--MIKNYPRKP 587

BLAST of CmUC03G063810 vs. ExPASy TrEMBL
Match: A0A0A0LXB9 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G604040 PE=3 SV=1)

HSP 1 Score: 1018.1 bits (2631), Expect = 1.5e-293
Identity = 479/591 (81.05%), Postives = 523/591 (88.49%), Query Frame = 0

Query: 1   MAKVANQPLFPMPFLVIFLVLSIMSGFGITLSEA-VPKVRHYKWEVEYMFWYPDCVENIV 60
           MAKVA++P FP PFL  FLVLSI+ GFGITLSEA  PK++HYKW+VEYMFW PDCVENIV
Sbjct: 1   MAKVADKPFFPKPFL-SFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIV 60

Query: 61  MGINGQFPGPTIRANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATN 120
           MGING+FPGPTIRAN GD +VVE+ NKL TEG+VIHWHGILQRGTPWADGTASISQCA N
Sbjct: 61  MGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120

Query: 121 PGETFIYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDINLLLSDW 180
           PGETF YRFVVDKAGTYFYHGHLGMQR+AGLYGSLIVD  EG+ EPFHYDE+INLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDW 180

Query: 181 WHKTVQKQEVGLNSNPMHWINEPQAILINGRGQTNCSIAAKYNTSLKQCELKGDEQCAPF 240
           WH++V KQEVGL+S PM WI EPQ+ILING+GQ +CSIAAKYN  LKQCEL G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPF 240

Query: 241 ILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSV 300
           ILHV+P KTYRIRIASTT+L++LNFAI NH+LLVVEADGNYVQPF+TSDIDIYSGESYSV
Sbjct: 241 ILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSV 300

Query: 301 LITTDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSK 360
           LITTDQ   ENYWVS+GVRAR PKTPP LT+LNYLPNS SKLPISPPPE P+W+D+DRSK
Sbjct: 301 LITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSK 360

Query: 361 NFTYRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIG 420
           NFT+RI AAMGSPKPPV+ NRR+FLLNTQN IN GF+KWAINNVSLALPPTPYLAA+++ 
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKMR 420

Query: 421 LNTTFDQNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSE 480
           LNT F+QNPPPETFP +YDI NPPPN  TTTGNGVY+F + E VDVILQNAN L    SE
Sbjct: 421 LNTAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSE 480

Query: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLGYGEGKF A  DEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WPFHCHIEPHLHMGMGVVFAEGVHKVDRIPTKALACGETAKVLINNPTKKP 591
           W FHCHIEPHLHMGMGVVFAEGVH V  IP KALACG TA  L+ N  + P
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTA--LVKNYPRLP 587

BLAST of CmUC03G063810 vs. TAIR 10
Match: AT5G21105.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 796.2 bits (2055), Expect = 1.8e-230
Identity = 366/575 (63.65%), Postives = 441/575 (76.70%), Query Frame = 0

Query: 18  FLVLSIMSGFGITLSEAV------PKVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTI 77
           F  +S M  + I L  AV        VR Y WEVEY +W PDC E  VM +NG+FPGPTI
Sbjct: 11  FTYISQMGVWWIVLVVAVLTHTASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTI 70

Query: 78  RANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGETFIYRFVVD 137
           +A  GDTIVV + NKL+TEGLVIHWHGI Q G+PWADG A ++QCA NPGETF Y F V+
Sbjct: 71  KAFAGDTIVVNLTNKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVE 130

Query: 138 KAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDINLLLSDWWHKTVQKQEVGL 197
           K GT+FYHGH GMQR+AGLYGSLIVD+ +GK E   YD + NLLLSDWWH+ +  QE+GL
Sbjct: 131 KPGTHFYHGHYGMQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGL 190

Query: 198 NSNPMHWINEPQAILINGRGQTNCSIAAKY--NTSLKQCELKGDEQCAPFILHVKPNKTY 257
           +S PM WI E Q+ILINGRGQ NCS+AA++  NTSL  C  K  +QCAP ILHV+PNKTY
Sbjct: 191 SSKPMRWIGEAQSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTY 250

Query: 258 RIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASE 317
           RIR++STT+L++LN A++ H+L+VVEADGNY+ PF T DIDIYSGESYSVL+TTDQ  S+
Sbjct: 251 RIRLSSTTALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQ 310

Query: 318 NYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSKNFTYRILAAM 377
           NY++SVGVR R P T  ALTILNY+    SKLP SPPP  P WDD++RSKNF+ +I +AM
Sbjct: 311 NYYISVGVRGRKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAM 370

Query: 378 GSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIGLNTTFDQNPP 437
           GSP PP K  +R+ LLNTQN I+ G+ KWAINNVSL  P TPYL +++  L   F++  P
Sbjct: 371 GSPSPPKKYRKRLILLNTQNLID-GYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSP 430

Query: 438 PETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSEIHPWHLHGHD 497
           P ++  DYDI NPPP  NTTTGNG+Y F     VDVI+QNAN L+   SEIHPWHLHGHD
Sbjct: 431 PRSYRMDYDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHD 490

Query: 498 FWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPH 557
           FWVLGYG+GKF   +DEK  NLKNPPLRNT +++PYGWTAIRFV DNPGVW FHCHIEPH
Sbjct: 491 FWVLGYGDGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPH 550

Query: 558 LHMGMGVVFAEGVHKVDRIPTKALACGETAKVLIN 585
           LHMGMGVVFAEG++++ ++P +AL CG T + L+N
Sbjct: 551 LHMGMGVVFAEGLNRIGKVPDEALGCGLTKQFLMN 584

BLAST of CmUC03G063810 vs. TAIR 10
Match: AT5G21100.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 740.0 bits (1909), Expect = 1.5e-213
Identity = 339/547 (61.97%), Postives = 423/547 (77.33%), Query Frame = 0

Query: 43  WEVEYMFWYPDCVENIVMGINGQFPGPTIRANVGDTIVVEMINKLSTEGLVIHWHGILQR 102
           WEVEY +W+PDC E IVM INGQFPGPTI A  GDT+++ ++NKLSTEG+VIHWHGI Q+
Sbjct: 28  WEVEYKYWWPDCKEGIVMAINGQFPGPTIDAVAGDTVIIHVVNKLSTEGVVIHWHGIRQK 87

Query: 103 GTPWADGTASISQCATNPGETFIYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIVDLEEGK 162
           GTPWADG A ++QC  NPGETF Y+F+VDKAGT+FYHGH GMQR++GLYG LIV      
Sbjct: 88  GTPWADGAAGVTQCPINPGETFTYKFIVDKAGTHFYHGHYGMQRSSGLYGMLIV---RSP 147

Query: 163 KEPFHYDEDINLLLSDWWHKTVQKQEVGLNSNPMHWINEPQAILINGRGQTNCSIAAKYN 222
           KE   YD + NLLLSDWWH+++  QE+ L+S PM WI EPQ++LINGRGQ NCS AA +N
Sbjct: 148 KERLIYDGEFNLLLSDWWHQSIHAQELALSSRPMRWIGEPQSLLINGRGQFNCSQAAYFN 207

Query: 223 TSLKQ--CELKGDEQCAPFILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVVEADGNY 282
              ++  C  K ++QCAP  L V+PN+ YR+RIASTT+L++LN A++ HQL+VVEADGNY
Sbjct: 208 KGGEKDVCTFKENDQCAPQTLRVEPNRVYRLRIASTTALASLNLAVQGHQLVVVEADGNY 267

Query: 283 VQPFLTSDIDIYSGESYSVLITTDQPASENYWVSVGVRARPPKTPPALTILNYLPNSVSK 342
           V PF  +DID+YSGE+YSVL+ T+   S+ YW+SVGVR R PKTP ALT++NY+  + S+
Sbjct: 268 VAPFTVNDIDVYSGETYSVLLKTNALPSKKYWISVGVRGREPKTPQALTVINYVDATESR 327

Query: 343 LPISPPPEIPNWDDYDRSKNFTYRILAAMGSPKPPVKSNRRIFLLNTQNHINGGFVKWAI 402
            P  PPP  P W+D DRSK+F+ +I AA G PKPP KS+ ++ LLNTQN +   + KW+I
Sbjct: 328 -PSHPPPVTPIWNDTDRSKSFSKKIFAAKGYPKPPEKSHDQLILLNTQN-LYEDYTKWSI 387

Query: 403 NNVSLALPPTPYLAAIRIGLNTTFD-QNPPPETFPEDYDIYNPPPNTNTTTGNGVYRFEL 462
           NNVSL++P TPYL +IR GL + +D ++P  +   ++YDI  PPPN NTT G+G+Y F  
Sbjct: 388 NNVSLSVPVTPYLGSIRYGLKSAYDLKSPAKKLIMDNYDIMKPPPNPNTTKGSGIYNFAF 447

Query: 463 WEIVDVILQNANTLEDKRSEIHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNT 522
             +VDVILQNAN L+   SEIHPWH+HGHDFWVLGYGEGKF   +DEK  NLKNPPLRNT
Sbjct: 448 GIVVDVILQNANVLKGVISEIHPWHIHGHDFWVLGYGEGKFKPGIDEKTFNLKNPPLRNT 507

Query: 523 VVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGMGVVFAEGVHKVDR--IPTKALACGE 582
           VV++P+GWTAIRFV DNPGVW FHCHIEPHLHMGMGVVF EGV ++ +  IP +AL CG 
Sbjct: 508 VVLYPFGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFVEGVDRIGKMEIPDEALGCGL 567

Query: 583 TAKVLIN 585
           T K L+N
Sbjct: 568 TRKWLMN 569

BLAST of CmUC03G063810 vs. TAIR 10
Match: AT5G21105.3 (Plant L-ascorbate oxidase )

HSP 1 Score: 725.3 bits (1871), Expect = 3.9e-209
Identity = 336/529 (63.52%), Postives = 402/529 (75.99%), Query Frame = 0

Query: 18  FLVLSIMSGFGITLSEAV------PKVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTI 77
           F  +S M  + I L  AV        VR Y WEVEY +W PDC E  VM +NG+FPGPTI
Sbjct: 11  FTYISQMGVWWIVLVVAVLTHTASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTI 70

Query: 78  RANVGDTIVVEMINKLSTEGLVIHWHGILQRGTPWADGTASISQCATNPGETFIYRFVVD 137
           +A  GDTIVV + NKL+TEGLVIHWHGI Q G+PWADG A ++QCA NPGETF Y F V+
Sbjct: 71  KAFAGDTIVVNLTNKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVE 130

Query: 138 KAGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEDINLLLSDWWHKTVQKQEVGL 197
           K GT+FYHGH GMQR+AGLYGSLIVD+ +GK E   YD + NLLLSDWWH+ +  QE+GL
Sbjct: 131 KPGTHFYHGHYGMQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGL 190

Query: 198 NSNPMHWINEPQAILINGRGQTNCSIAAKY--NTSLKQCELKGDEQCAPFILHVKPNKTY 257
           +S PM WI E Q+ILINGRGQ NCS+AA++  NTSL  C  K  +QCAP ILHV+PNKTY
Sbjct: 191 SSKPMRWIGEAQSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTY 250

Query: 258 RIRIASTTSLSALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASE 317
           RIR++STT+L++LN A++ H+L+VVEADGNY+ PF T DIDIYSGESYSVL+TTDQ  S+
Sbjct: 251 RIRLSSTTALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQ 310

Query: 318 NYWVSVGVRARPPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSKNFTYRILAAM 377
           NY++SVGVR R P T  ALTILNY+    SKLP SPPP  P WDD++RSKNF+ +I +AM
Sbjct: 311 NYYISVGVRGRKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAM 370

Query: 378 GSPKPPVKSNRRIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIGLNTTFDQNPP 437
           GSP PP K  +R+ LLNTQN I+ G+ KWAINNVSL  P TPYL +++  L   F++  P
Sbjct: 371 GSPSPPKKYRKRLILLNTQNLID-GYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSP 430

Query: 438 PETFPEDYDIYNPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSEIHPWHLHGHD 497
           P ++  DYDI NPPP  NTTTGNG+Y F     VDVI+QNAN L+   SEIHPWHLHGHD
Sbjct: 431 PRSYRMDYDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHD 490

Query: 498 FWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPG 539
           FWVLGYG+GKF   +DEK  NLKNPPLRNT +++PYGWTAIRFV DNPG
Sbjct: 491 FWVLGYGDGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPG 538

BLAST of CmUC03G063810 vs. TAIR 10
Match: AT4G39830.1 (Cupredoxin superfamily protein )

HSP 1 Score: 578.9 bits (1491), Expect = 4.5e-165
Identity = 276/551 (50.09%), Postives = 358/551 (64.97%), Query Frame = 0

Query: 37  KVRHYKWEVEYMFWYPDCVENIVMGINGQFPGPTIRANVGDTIVVEMINKLSTEGLVIHW 96
           K+R +KWEV+Y F  PDC E +V+ ING+FPGPTI+A  GDTIVVE+ N   TE + +HW
Sbjct: 34  KIRRFKWEVKYEFKSPDCFEKLVITINGKFPGPTIKAQQGDTIVVELKNSFMTENVAVHW 93

Query: 97  HGILQRGTPWADGTASISQCATNPGETFIYRFVVDKAGTYFYHGHLGMQRAAGLYGSLIV 156
           HGI Q GTPW DG   ++QC   PGE FIY+FVVD+ GTY YH H GMQR +GL G + V
Sbjct: 94  HGIRQIGTPWFDGVEGVTQCPILPGEVFIYQFVVDRPGTYMYHSHYGMQRESGLIGMIQV 153

Query: 157 DLEEGKKEPFHYDEDINLLLSDWWHKTVQKQEVGLNSNPMHWINEPQAILINGRGQTNCS 216
                + EPF YD D N LL+DW+HK++ ++  GL S P  W+ EPQ+++I GRG+ NCS
Sbjct: 154 SPPATEPEPFTYDYDRNFLLTDWYHKSMSEKATGLASIPFKWVGEPQSLMIQGRGRFNCS 213

Query: 217 IAAKYNTSLKQ--CELKGDEQCAPFILHVKPNKTYRIRIASTTSLSALNFAIRNHQLLVV 276
                  SL    C +  +  C+ FIL V P KTYR+RI S T+LSAL+F I  H L VV
Sbjct: 214 NNLTTPPSLVSGVCNV-SNADCSRFILTVIPGKTYRLRIGSLTALSALSFQIEGHNLTVV 273

Query: 277 EADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENYWVSVGVRARPPKTPPALTILNYL 336
           EADG+YV+PF   ++ +YSGE+YSVL+  DQ    NYW++  + +RP  TPPA  +LNY 
Sbjct: 274 EADGHYVEPFTVKNLFVYSGETYSVLLKADQNPRRNYWITSSIVSRPATTPPATAVLNYY 333

Query: 337 PNSVSKLPISPPPE--IPNWDDYDRSKNFTYRILAAMGSPKP-PVKSNRRIFLLNTQNHI 396
           PN   + P +      +P W+D       +  I A  G     P  S++ I LLNTQN +
Sbjct: 334 PNHPRRRPPTSESSNIVPEWNDTRSRLAQSLAIKARRGFIHALPENSDKVIVLLNTQNEV 393

Query: 397 NGGFVKWAINNVSLALPPTPYLAAIRIGLNTTFD-QNPPPETF-PEDYDIYNPPPNTNTT 456
           N G+ +W++NNVS   P TPYL A++  L   FD +   PE +   +YDI+  P N N T
Sbjct: 394 N-GYRRWSVNNVSYHHPKTPYLIALKQNLTNAFDWRFTAPENYDSRNYDIFAKPLNANAT 453

Query: 457 TGNGVYRFELWEIVDVILQNANTLEDKRSEIHPWHLHGHDFWVLGYGEGKFSAAVDEKKL 516
           T +G+YR      VDVILQNANT+    SE HPWHLHGHDFWVLGYGEGKF+ + D K+ 
Sbjct: 454 TSDGIYRLRFNSTVDVILQNANTMNANNSETHPWHLHGHDFWVLGYGEGKFNESEDPKRY 513

Query: 517 NLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGMGVVFAEGVHKVDRIP 576
           N  +P  +NTV + P+GWTA+RF ADNPGVW FHCHIE H  MGMG+VF  G+ KV  +P
Sbjct: 514 NRVDPIKKNTVAVQPFGWTALRFRADNPGVWSFHCHIESHFFMGMGIVFESGIDKVSSLP 573

Query: 577 TKALACGETAK 581
           +  + CG+T +
Sbjct: 574 SSIMGCGQTKR 582

BLAST of CmUC03G063810 vs. TAIR 10
Match: AT5G21105.2 (Plant L-ascorbate oxidase )

HSP 1 Score: 541.6 bits (1394), Expect = 8.0e-154
Identity = 248/384 (64.58%), Postives = 303/384 (78.91%), Query Frame = 0

Query: 203 QAILINGRGQTNCSIAAKY--NTSLKQCELKGDEQCAPFILHVKPNKTYRIRIASTTSLS 262
           ++ILINGRGQ NCS+AA++  NTSL  C  K  +QCAP ILHV+PNKTYRIR++STT+L+
Sbjct: 11  KSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALA 70

Query: 263 ALNFAIRNHQLLVVEADGNYVQPFLTSDIDIYSGESYSVLITTDQPASENYWVSVGVRAR 322
           +LN A++ H+L+VVEADGNY+ PF T DIDIYSGESYSVL+TTDQ  S+NY++SVGVR R
Sbjct: 71  SLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGR 130

Query: 323 PPKTPPALTILNYLPNSVSKLPISPPPEIPNWDDYDRSKNFTYRILAAMGSPKPPVKSNR 382
            P T  ALTILNY+    SKLP SPPP  P WDD++RSKNF+ +I +AMGSP PP K  +
Sbjct: 131 KPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRK 190

Query: 383 RIFLLNTQNHINGGFVKWAINNVSLALPPTPYLAAIRIGLNTTFDQNPPPETFPEDYDIY 442
           R+ LLNTQN I+ G+ KWAINNVSL  P TPYL +++  L   F++  PP ++  DYDI 
Sbjct: 191 RLILLNTQNLID-GYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIM 250

Query: 443 NPPPNTNTTTGNGVYRFELWEIVDVILQNANTLEDKRSEIHPWHLHGHDFWVLGYGEGKF 502
           NPPP  NTTTGNG+Y F     VDVI+QNAN L+   SEIHPWHLHGHDFWVLGYG+GKF
Sbjct: 251 NPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKF 310

Query: 503 SAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWPFHCHIEPHLHMGMGVVFAE 562
              +DEK  NLKNPPLRNT +++PYGWTAIRFV DNPGVW FHCHIEPHLHMGMGVVFAE
Sbjct: 311 KPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAE 370

Query: 563 GVHKVDRIPTKALACGETAKVLIN 585
           G++++ ++P +AL CG T + L+N
Sbjct: 371 GLNRIGKVPDEALGCGLTKQFLMN 393

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038880052.10.0e+0084.41L-ascorbate oxidase-like [Benincasa hispida][more]
XP_038878978.12.9e-30784.97L-ascorbate oxidase [Benincasa hispida][more]
NP_001284440.13.3e-30382.82L-ascorbate oxidase-like [Cucumis melo] >AAF35910.1 ascorbate oxidase AO1 [Cucum... [more]
TYK09892.14.8e-30282.82L-ascorbate oxidase-like [Cucumis melo var. makuwa][more]
KAA0055636.14.8e-29481.56L-ascorbate oxidase [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
P141334.1e-29681.05L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1[more]
P247922.9e-27878.53L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2[more]
P370641.8e-27579.82L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1[more]
Q405881.2e-23666.14L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1[more]
M4DUF24.8e-22865.69L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1[more]
Match NameE-valueIdentityDescription
Q9M5B51.6e-30382.82L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=2C07 PE=2 SV=1[more]
A0A5D3CF112.3e-30282.82L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G0... [more]
A0A5A7UMY32.3e-29481.56L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G... [more]
Q9M5B41.2e-29381.39L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492479 PE=2 SV=1[more]
A0A0A0LXB91.5e-29381.05L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G604040 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G21105.11.8e-23063.65Plant L-ascorbate oxidase [more]
AT5G21100.11.5e-21361.97Plant L-ascorbate oxidase [more]
AT5G21105.33.9e-20963.52Plant L-ascorbate oxidase [more]
AT4G39830.14.5e-16550.09Cupredoxin superfamily protein [more]
AT5G21105.28.0e-15464.58Plant L-ascorbate oxidase [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 446..561
e-value: 9.4E-35
score: 119.3
IPR008972CupredoxinGENE3D2.60.40.420coord: 372..589
e-value: 1.3E-72
score: 245.9
IPR008972CupredoxinGENE3D2.60.40.420coord: 166..371
e-value: 2.4E-65
score: 222.4
IPR008972CupredoxinGENE3D2.60.40.420coord: 28..157
e-value: 2.4E-46
score: 159.4
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 374..575
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 165..370
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 34..183
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 172..334
e-value: 1.7E-47
score: 161.5
IPR017760L-ascorbate oxidase, plantsTIGRFAMTIGR03388TIGR03388coord: 38..578
e-value: 1.1E-296
score: 982.7
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 45..158
e-value: 2.7E-39
score: 133.9
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 32..579
NoneNo IPR availablePANTHERPTHR11709:SF394PLANT L-ASCORBATE OXIDASEcoord: 32..579
IPR002355Multicopper oxidase, copper-binding sitePROSITEPS00080MULTICOPPER_OXIDASE2coord: 543..554
IPR033138Multicopper oxidases, conserved sitePROSITEPS00079MULTICOPPER_OXIDASE1coord: 538..558
IPR034259Ascorbate oxidase, first cupredoxin domainCDDcd13845CuRO_1_AAOcoord: 39..158
e-value: 7.75185E-82
score: 250.441
IPR034267Ascorbate oxidase, third cupredoxin domainCDDcd13893CuRO_3_AAOcoord: 378..569
e-value: 8.11812E-83
score: 254.267

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmUC03G063810.1CmUC03G063810.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005576 extracellular region
molecular_function GO:0005507 copper ion binding
molecular_function GO:0008447 L-ascorbate oxidase activity
molecular_function GO:0016491 oxidoreductase activity