CmUC03G060470 (gene) Watermelon (USVL531) v1

Overview
NameCmUC03G060470
Typegene
OrganismCitrullus mucosospermus (Watermelon (USVL531) v1)
Description(1->3)-beta-glucan endohydrolase
LocationCmU531Chr03: 19289523 .. 19292285 (+)
RNA-Seq ExpressionCmUC03G060470
SyntenyCmUC03G060470
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCCTCAACATACAAACCCCAATCTTTTCTTACATATCCAATGGCAAACTTTTGTTCCATCATTTCCTTATGCCTTATTGGCCTCTTTCTGCCAAACCTACATCTAACAAGTATCTCTCTTCCTCTCATATTGCATTATTGTATTATCAATTTTTTTTTATTAAACTAATTTTTTGTTTTTATTTTTGCAATGCTTCTTAACTTTCTATGCAAAATCTAGAAATCTAGTTTGCTATGTTCATGGATCTTCATTGTTTTTGTTTTTCTTATTATGAAAAGTTATTTGCTCTATATTTTAAATCTTATTAAAGTTTCAATACGAAAGGTCAAAAGACCTCCTTCAATAATTATTTGATTTTTAATTTTTTATTTTAAAAAATTAAGTTCTATATATATAGCTTGTGGCCATGCATCAAATTATTTATTTTTAATTTTTTCTTCTCTTATCGACATTTAATTTAGTAAACTATATATATATAATAAATGCTTTTAAAAACTTTTTTTTTTCTACTTTTAGAATTTGGCCGAGATTTAAAGGTTTTAATAGAAAATATAAAAATCATTCTAAAAGAAATACACAAGTTTCTAAACTCAAAAATTTAAAAATCAAATGATAACCAACATAACATAAATTTATTTATAAGATTTTGTTTGACAAATAACAATAGTGATTTTGTTTCGTTATTATAGTTATTGTTAATAGATTTGTTTCATGACACGTCGTAAGGTTTGGTTTTCCATTTTTTAAAGTTGTACTCTTACCATCTTTGATAAAACTTAGAAAACAAACATGGGTTTGCAATGAGTGTTTCAATTTTTTTTTTAGAAAATATAAAATAAAGAAATATTTTAGTGAAGAAGCATTATTTGAGTTTTTTTTTTTTTTAAAAAGGTAAAAAATACATTTTTGTATTAATAAATAAATTATTCTTTTGGAAATAAAAATAGATAAATATTTTTATTCACTAAATTTGTTACTTTAATTTTTACTTTCAAAATATAAGCCTGTAAATTAATCAAACTAATATACAACCCATATTATACCCCCACTAAATACCTACGATATGTGAGTTTTAGTAGTTCAACATTTAATATGACTCAGTTATACTCCCACTAAATATCTACAATATGTGAGCTTTAGTGGTCCATCAACATTTAATATGACCCTTAATAATCATATAATTTCCTCCTTAATATGACTCATACATAAAGATAGCACTATAATAGATAGTATACACGACTTTCATTTAACATTTCACATATTATTAGATGATATATTTTTTAAAAAGTCCACTAGAAATGATAGAAATTTTCACAAGGTTAAGTCTCTTGTCACTTAGAACGAAGACAATAACAAGACTTGGATGAAGACAAAAGTTTCTCCAATTTATTTTCAAGGATGGATATAAGGAATGCATCACCCTCATCCTAACCTTGATTCCAAACTCAATTATAATTAGTTAGTTATTTTTGCTTTTATTAACTTTTTATATTAAAGTGATAATAAAAATGTTAGAATTTTTAGAGACTGATTATTTAAATAAATAAAATAATTAAAAAAAAGTTACTAATTTTAACATAAAACTATATATAATTTGAGTATATTCATTGAATTTCAATTATTTTTTATTTAAAAAATTATTAAATATAGAAACGAAAAAGAGGTTGTATGCCATAATAGTAGAAATTTTATTTATAATTTAAAATATGTTGTGGTAATTTGAGGTGCACAAATTGGAGTTTGCTATGGACAAATGGGAAACAACCTTCCAACCCAAAGAGAAGTGGTAGATCTCTACAACCAAAGAAACATTAGAAGAATGAGACTGTACGATCCAAATAAAGCTTCCCTGGATGCTCTTAGAGGCTCCAACATCGAGCTCATGCTGGGCCTTCCCAATACCGACCTCCAAAGAATCGCTTCAAGCCAGGCTGAAGCCAATACGTGGGTTCAAAACAATGTAAAAAATTTCGCCAACGTGAGATTCAAGTACATCGCCGTTGGCAATGAAGTCCAACCCTCTAGTTCTACTGCCCAGTTCCTGGTCCCAGCGATGCGAAACATCCAAACTGCAGTTTCCAACGCTGGCTTGGCTGATCGGATCAAAGTTTCCACCGCCGTTGACACCAGTATCATTGGCGTGTCCTTCCCTCCTTCTCGTGGCGCATTCAAAAACGAAGTCATGCCTCTAATGAACTCCATCATTAGTTTTCTAGTAGCCAATCGTTCCCCACTACTTGTCAACTTGTACCCTTACTTCAGTTACATTGGCAACCCAAGGGACATTCGTTTAGACTATGCCCTCTTCACGGCACCGTCAACTGTGGTCACTGATGGCCAGTTTCGTTACCAGAATTTGTTTGATGCAATTCTTGATGCAGTTTACGCAGCATTGGAGAAGGTTGGCGGAGCAAATATAAACATTGTGATATCAGAGAGTGGGTGGCCATCCGCGGGGGGGACGGCTACTTCAATCGATAATGCAAGGACTTACATTAACAATTTGATTCAACATGTTAAGAACGGGACTCCCAGACGCCCTCAAAGGCCAATTGAGACTTATATTTTTGGCATGTTTGATGAGAACCAGAAGAATCCTGAGTTAGAGAAGCATTTTGGCCTCTTCTTTCCAAACAAACAGCCTAAATTTCCCGTCAATTTCAATTGATAGACTTGGAATCGATATTGATATAAGGAGAAATTAAAGCTTTCAAATAAACTCCTCGAGATTGCAATGCTTGAAATACTACATATTATATTTT

mRNA sequence

ATCCTCAACATACAAACCCCAATCTTTTCTTACATATCCAATGGCAAACTTTTGTTCCATCATTTCCTTATGCCTTATTGGCCTCTTTCTGCCAAACCTACATCTAACAAGTGCACAAATTGGAGTTTGCTATGGACAAATGGGAAACAACCTTCCAACCCAAAGAGAAGTGGTAGATCTCTACAACCAAAGAAACATTAGAAGAATGAGACTGTACGATCCAAATAAAGCTTCCCTGGATGCTCTTAGAGGCTCCAACATCGAGCTCATGCTGGGCCTTCCCAATACCGACCTCCAAAGAATCGCTTCAAGCCAGGCTGAAGCCAATACGTGGGTTCAAAACAATGTAAAAAATTTCGCCAACGTGAGATTCAAGTACATCGCCGTTGGCAATGAAGTCCAACCCTCTAGTTCTACTGCCCAGTTCCTGGTCCCAGCGATGCGAAACATCCAAACTGCAGTTTCCAACGCTGGCTTGGCTGATCGGATCAAAGTTTCCACCGCCGTTGACACCAGTATCATTGGCGTGTCCTTCCCTCCTTCTCGTGGCGCATTCAAAAACGAAGTCATGCCTCTAATGAACTCCATCATTAGTTTTCTAGTAGCCAATCGTTCCCCACTACTTGTCAACTTGTACCCTTACTTCAGTTACATTGGCAACCCAAGGGACATTCGTTTAGACTATGCCCTCTTCACGGCACCGTCAACTGTGGTCACTGATGGCCAGTTTCGTTACCAGAATTTGTTTGATGCAATTCTTGATGCAGTTTACGCAGCATTGGAGAAGGTTGGCGGAGCAAATATAAACATTGTGATATCAGAGAGTGGGTGGCCATCCGCGGGGGGGACGGCTACTTCAATCGATAATGCAAGGACTTACATTAACAATTTGATTCAACATGTTAAGAACGGGACTCCCAGACGCCCTCAAAGGCCAATTGAGACTTATATTTTTGGCATGTTTGATGAGAACCAGAAGAATCCTGAGTTAGAGAAGCATTTTGGCCTCTTCTTTCCAAACAAACAGCCTAAATTTCCCGTCAATTTCAATTGATAGACTTGGAATCGATATTGATATAAGGAGAAATTAAAGCTTTCAAATAAACTCCTCGAGATTGCAATGCTTGAAATACTACATATTATATTTT

Coding sequence (CDS)

ATGGCAAACTTTTGTTCCATCATTTCCTTATGCCTTATTGGCCTCTTTCTGCCAAACCTACATCTAACAAGTGCACAAATTGGAGTTTGCTATGGACAAATGGGAAACAACCTTCCAACCCAAAGAGAAGTGGTAGATCTCTACAACCAAAGAAACATTAGAAGAATGAGACTGTACGATCCAAATAAAGCTTCCCTGGATGCTCTTAGAGGCTCCAACATCGAGCTCATGCTGGGCCTTCCCAATACCGACCTCCAAAGAATCGCTTCAAGCCAGGCTGAAGCCAATACGTGGGTTCAAAACAATGTAAAAAATTTCGCCAACGTGAGATTCAAGTACATCGCCGTTGGCAATGAAGTCCAACCCTCTAGTTCTACTGCCCAGTTCCTGGTCCCAGCGATGCGAAACATCCAAACTGCAGTTTCCAACGCTGGCTTGGCTGATCGGATCAAAGTTTCCACCGCCGTTGACACCAGTATCATTGGCGTGTCCTTCCCTCCTTCTCGTGGCGCATTCAAAAACGAAGTCATGCCTCTAATGAACTCCATCATTAGTTTTCTAGTAGCCAATCGTTCCCCACTACTTGTCAACTTGTACCCTTACTTCAGTTACATTGGCAACCCAAGGGACATTCGTTTAGACTATGCCCTCTTCACGGCACCGTCAACTGTGGTCACTGATGGCCAGTTTCGTTACCAGAATTTGTTTGATGCAATTCTTGATGCAGTTTACGCAGCATTGGAGAAGGTTGGCGGAGCAAATATAAACATTGTGATATCAGAGAGTGGGTGGCCATCCGCGGGGGGGACGGCTACTTCAATCGATAATGCAAGGACTTACATTAACAATTTGATTCAACATGTTAAGAACGGGACTCCCAGACGCCCTCAAAGGCCAATTGAGACTTATATTTTTGGCATGTTTGATGAGAACCAGAAGAATCCTGAGTTAGAGAAGCATTTTGGCCTCTTCTTTCCAAACAAACAGCCTAAATTTCCCGTCAATTTCAATTGA

Protein sequence

MANFCSIISLCLIGLFLPNLHLTSAQIGVCYGQMGNNLPTQREVVDLYNQRNIRRMRLYDPNKASLDALRGSNIELMLGLPNTDLQRIASSQAEANTWVQNNVKNFANVRFKYIAVGNEVQPSSSTAQFLVPAMRNIQTAVSNAGLADRIKVSTAVDTSIIGVSFPPSRGAFKNEVMPLMNSIISFLVANRSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVTDGQFRYQNLFDAILDAVYAALEKVGGANINIVISESGWPSAGGTATSIDNARTYINNLIQHVKNGTPRRPQRPIETYIFGMFDENQKNPELEKHFGLFFPNKQPKFPVNFN
Homology
BLAST of CmUC03G060470 vs. NCBI nr
Match: XP_038876764.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Benincasa hispida])

HSP 1 Score: 573.9 bits (1478), Expect = 8.9e-160
Identity = 281/337 (83.38%), Postives = 310/337 (91.99%), Query Frame = 0

Query: 1   MANFCSIISLCLIGLFLPNLHLTSAQIGVCYGQMGNNLPTQREVVDLYNQRNIRRMRLYD 60
           MANF SIISL L+G  LPNLHLTSAQIGVCYGQ+GNNLP+QREVV+L+NQRNIRRMRLYD
Sbjct: 1   MANFYSIISLFLLGFLLPNLHLTSAQIGVCYGQLGNNLPSQREVVNLFNQRNIRRMRLYD 60

Query: 61  PNKASLDALRGSNIELMLGLPNTDLQRIASSQAEANTWVQNNVKNFANVRFKYIAVGNEV 120
           PN+ASLDALRGSNIELMLGLPN+DLQRIASSQ+EAN WVQNNVK+F NVRFKYIAVGNEV
Sbjct: 61  PNRASLDALRGSNIELMLGLPNSDLQRIASSQSEANAWVQNNVKSFGNVRFKYIAVGNEV 120

Query: 121 QPSSSTAQFLVPAMRNIQTAVSNAGLADRIKVSTAVDTSIIGVSFPPSRGAFKNEVMPLM 180
           QPSSS AQF+VPAM+NIQTA+SN GLA++IKVSTA+DT ++GVSFPPS GAF N+ MPL+
Sbjct: 121 QPSSSAAQFVVPAMQNIQTALSNGGLANQIKVSTAIDTGLLGVSFPPSSGAFNNQAMPLL 180

Query: 181 NSIISFLVANRSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVTDGQFRYQNLFDAIL 240
           N II FL ANRSPLLVNLYP+FSYI NPRDIRLDYALFTA STVVTDGQ RYQNLFDAI+
Sbjct: 181 NPIIRFLNANRSPLLVNLYPFFSYISNPRDIRLDYALFTAKSTVVTDGQLRYQNLFDAIV 240

Query: 241 DAVYAALEKVGGANINIVISESGWPSAGGTATSIDNARTYINNLIQHVKNGTPRRPQRPI 300
           DA+YAALEK GG+++ IV+SESGWPSAGGTA S +NARTY NNLIQHVK GTPRRPQRPI
Sbjct: 241 DAIYAALEKAGGSSLEIVVSESGWPSAGGTAASTENARTYNNNLIQHVKRGTPRRPQRPI 300

Query: 301 ETYIFGMFDENQKNPELEKHFGLFFPNKQPKFPVNFN 338
           ETYIF MFDENQK+PE EKHFGLFFPNKQPK+ VNFN
Sbjct: 301 ETYIFAMFDENQKSPEFEKHFGLFFPNKQPKYAVNFN 337

BLAST of CmUC03G060470 vs. NCBI nr
Match: XP_008443726.1 (PREDICTED: glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo])

HSP 1 Score: 565.1 bits (1455), Expect = 4.1e-157
Identity = 283/339 (83.48%), Postives = 303/339 (89.38%), Query Frame = 0

Query: 1   MANFC-SIISLCLIGLFLPNLHLTSAQIGVCYGQMGNNLPTQREVVDLYNQRNIRRMRLY 60
           MANF  ++I LCL+GL + NLHLTSAQIGVCYGQMGNNLP QREVV LYNQ NI+RMRLY
Sbjct: 1   MANFSWTMICLCLLGLLVQNLHLTSAQIGVCYGQMGNNLPPQREVVTLYNQNNIKRMRLY 60

Query: 61  DPNKASLDALRGSNIELMLGLPNTDLQRIASSQAEANTWVQNNVKNFANVRFKYIAVGNE 120
           DPN+ SLDALRGS IELMLGLPN DLQRIASSQAEAN WVQNN+KN+ NVRFKYIAVGNE
Sbjct: 61  DPNRGSLDALRGSTIELMLGLPNPDLQRIASSQAEANAWVQNNIKNYQNVRFKYIAVGNE 120

Query: 121 VQPSSSTAQFLVPAMRNIQTAVSNAGLADRIKVSTAVDTSIIGVSFPPSRGAFKNEVMPL 180
           VQPSSS A F+VPAMRNIQTA++NAGL +RIKVSTA+DTSI+ +S+PPS G  KNE MP 
Sbjct: 121 VQPSSSAASFVVPAMRNIQTALNNAGLVNRIKVSTAIDTSILAMSYPPSSGTIKNEDMPF 180

Query: 181 MNSIISFLVANRSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVTDGQFRYQNLFDAI 240
           M+S+I FL  N SPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVV DGQFRYQNLFDAI
Sbjct: 181 MSSVIRFLKDNGSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQFRYQNLFDAI 240

Query: 241 LDAVYAALEKVGGANINIVISESGWPSAGGTATSIDNARTYINNLIQHVKNGTPRRP-QR 300
           LDAVYAALEKVGG N+ IVISESGWPSAGGTA SIDNARTYINNLIQHVK GTPRRP  R
Sbjct: 241 LDAVYAALEKVGGGNLEIVISESGWPSAGGTAASIDNARTYINNLIQHVKRGTPRRPGGR 300

Query: 301 PIETYIFGMFDENQKNPELEKHFGLFFPNKQPKFPVNFN 338
           PIETYIF MFDENQKNPE EKHFGLFFPNKQ K+PVNFN
Sbjct: 301 PIETYIFAMFDENQKNPEFEKHFGLFFPNKQSKYPVNFN 339

BLAST of CmUC03G060470 vs. NCBI nr
Match: XP_031737288.1 (glucan endo-1,3-beta-glucosidase, basic isoform [Cucumis sativus] >KGN66700.1 hypothetical protein Csa_007701 [Cucumis sativus])

HSP 1 Score: 555.1 bits (1429), Expect = 4.3e-154
Identity = 276/337 (81.90%), Postives = 302/337 (89.61%), Query Frame = 0

Query: 1   MANFCSIISLCLIGLFLPNLHLTSAQIGVCYGQMGNNLPTQREVVDLYNQRNIRRMRLYD 60
           MANF  IISLCL+GL +PNLHLT+AQIGVCYGQMGNNLP Q EVV L+NQ NI+RMRLYD
Sbjct: 1   MANFSPIISLCLLGLLVPNLHLTNAQIGVCYGQMGNNLPPQTEVVTLFNQNNIKRMRLYD 60

Query: 61  PNKASLDALRGSNIELMLGLPNTDLQRIASSQAEANTWVQNNVKNFANVRFKYIAVGNEV 120
           PN+ SLDALRGS IELMLG+PN+DLQRIASSQ EAN WVQNNVKN+ NVRF+YIAVGNEV
Sbjct: 61  PNRGSLDALRGSPIELMLGVPNSDLQRIASSQTEANAWVQNNVKNYPNVRFRYIAVGNEV 120

Query: 121 QPSSSTAQFLVPAMRNIQTAVSNAGLADRIKVSTAVDTSIIGVSFPPSRGAFKNEVMPLM 180
           QPSSS A F+VPAM NIQTA++NAGL  +IKVSTAV TSI+  S+PPSRG  KNEVMPLM
Sbjct: 121 QPSSSAASFVVPAMVNIQTALNNAGLG-KIKVSTAVATSIMADSYPPSRGTIKNEVMPLM 180

Query: 181 NSIISFLVANRSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVTDGQFRYQNLFDAIL 240
           N II FL  NRSPLL+NLYPYFSYIGNPRDIRLDYALFTAPSTVV DGQ+ YQNLFDA+L
Sbjct: 181 NPIIRFLNNNRSPLLLNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQYLYQNLFDAML 240

Query: 241 DAVYAALEKVGGANINIVISESGWPSAGGTATSIDNARTYINNLIQHVKNGTPRRPQRPI 300
           DA+YAALEKVGG N+ IVISESGWPSAGGTATSI+NARTYINNLIQHVK GTPRRP RP 
Sbjct: 241 DALYAALEKVGGGNLEIVISESGWPSAGGTATSINNARTYINNLIQHVKRGTPRRPGRPT 300

Query: 301 ETYIFGMFDENQKNPELEKHFGLFFPNKQPKFPVNFN 338
           ETYIF MFDEN+K+PELEKHFGLFFPNKQ K+P+NFN
Sbjct: 301 ETYIFAMFDENKKSPELEKHFGLFFPNKQSKYPINFN 336

BLAST of CmUC03G060470 vs. NCBI nr
Match: XP_022960641.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucurbita moschata])

HSP 1 Score: 550.4 bits (1417), Expect = 1.1e-152
Identity = 270/337 (80.12%), Postives = 297/337 (88.13%), Query Frame = 0

Query: 1   MANFCSIISLCLIGLFLPNLHLTSAQIGVCYGQMGNNLPTQREVVDLYNQRNIRRMRLYD 60
           MAN  SI+SLCL+GL +P+LHLTSAQIGVCYGQMGNNLP  REVV LYNQ NIRRMRLY 
Sbjct: 1   MANSYSIVSLCLLGLLMPSLHLTSAQIGVCYGQMGNNLPPSREVVALYNQNNIRRMRLYG 60

Query: 61  PNKASLDALRGSNIELMLGLPNTDLQRIASSQAEANTWVQNNVKNFANVRFKYIAVGNEV 120
           PN+ SL AL GSNIEL+L +PN DL +IASSQAEANTWVQNN+++  NVRFKYIAVGNEV
Sbjct: 61  PNRDSLGALSGSNIELILDVPNPDLPKIASSQAEANTWVQNNIRSLPNVRFKYIAVGNEV 120

Query: 121 QPSSSTAQFLVPAMRNIQTAVSNAGLADRIKVSTAVDTSIIGVSFPPSRGAFKNEVMPLM 180
           Q SSS AQF+VPAMRNIQTA+++ GL  RIKVSTA+DT ++G SFPPS GAFK+E MPL+
Sbjct: 121 QSSSSNAQFVVPAMRNIQTALASVGLGGRIKVSTAIDTGVLGTSFPPSNGAFKSEAMPLL 180

Query: 181 NSIISFLVANRSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVTDGQFRYQNLFDAIL 240
           N II FL+ N SPLLVNLYPYFSYIGN RDIRLDYALFTAPSTVVTDGQFRYQNLFDAIL
Sbjct: 181 NPIIRFLIENGSPLLVNLYPYFSYIGNMRDIRLDYALFTAPSTVVTDGQFRYQNLFDAIL 240

Query: 241 DAVYAALEKVGGANINIVISESGWPSAGGTATSIDNARTYINNLIQHVKNGTPRRPQRPI 300
           DA YAALEK GG N+ IV+SESGWP+AGGTATSIDNARTYINNLIQHVK GTPRRP +PI
Sbjct: 241 DATYAALEKAGGRNVEIVVSESGWPTAGGTATSIDNARTYINNLIQHVKRGTPRRPGKPI 300

Query: 301 ETYIFGMFDENQKNPELEKHFGLFFPNKQPKFPVNFN 338
           ETYIF MFDENQKNPE EKHFGLF PNKQ K+P+NFN
Sbjct: 301 ETYIFAMFDENQKNPEFEKHFGLFSPNKQLKYPINFN 337

BLAST of CmUC03G060470 vs. NCBI nr
Match: XP_022987104.1 (glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucurbita maxima])

HSP 1 Score: 550.1 bits (1416), Expect = 1.4e-152
Identity = 272/337 (80.71%), Postives = 295/337 (87.54%), Query Frame = 0

Query: 1   MANFCSIISLCLIGLFLPNLHLTSAQIGVCYGQMGNNLPTQREVVDLYNQRNIRRMRLYD 60
           MAN  SIISLCL+GL +  LHLTSAQIGVCYGQMGNNLP  REVV LYNQ NIRRMRLY 
Sbjct: 1   MANSYSIISLCLLGLLMSTLHLTSAQIGVCYGQMGNNLPPSREVVALYNQNNIRRMRLYG 60

Query: 61  PNKASLDALRGSNIELMLGLPNTDLQRIASSQAEANTWVQNNVKNFANVRFKYIAVGNEV 120
           PN+ SL AL GSNIEL+L +PN DLQRIASSQAEANTWVQNN+++F NVRFKYI+VGNEV
Sbjct: 61  PNRDSLGALSGSNIELILDVPNPDLQRIASSQAEANTWVQNNIRSFPNVRFKYISVGNEV 120

Query: 121 QPSSSTAQFLVPAMRNIQTAVSNAGLADRIKVSTAVDTSIIGVSFPPSRGAFKNEVMPLM 180
           QPSSS AQF+VPAMRNIQTA++N GL+ RIKVST++DT +IG SFPPS GAFK+E MPL+
Sbjct: 121 QPSSSNAQFVVPAMRNIQTALANVGLSSRIKVSTSIDTGVIGTSFPPSNGAFKSEAMPLL 180

Query: 181 NSIISFLVANRSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVTDGQFRYQNLFDAIL 240
           N II FL  N SPLLVNLYPYFSYIGN RDIRLDYALFTAPSTVVTDGQFRYQNLFDAIL
Sbjct: 181 NPIIRFLNENSSPLLVNLYPYFSYIGNMRDIRLDYALFTAPSTVVTDGQFRYQNLFDAIL 240

Query: 241 DAVYAALEKVGGANINIVISESGWPSAGGTATSIDNARTYINNLIQHVKNGTPRRPQRPI 300
           DA YAALEK GG N+ IV+SESGWP+AGG ATSI NARTYINNLIQHVK GTPRRP RPI
Sbjct: 241 DATYAALEKAGGRNLEIVVSESGWPTAGGAATSISNARTYINNLIQHVKRGTPRRPGRPI 300

Query: 301 ETYIFGMFDENQKNPELEKHFGLFFPNKQPKFPVNFN 338
           E YIF MFDENQKNPE EKHFGLF PNKQ K+P+NFN
Sbjct: 301 EAYIFAMFDENQKNPEFEKHFGLFTPNKQLKYPINFN 337

BLAST of CmUC03G060470 vs. ExPASy Swiss-Prot
Match: P52408 (Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS1 PE=3 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 9.3e-128
Identity = 227/331 (68.58%), Postives = 272/331 (82.18%), Query Frame = 0

Query: 7   IISLCLIGLFLPNLHLTSAQIGVCYGQMGNNLPTQREVVDLYNQRNIRRMRLYDPNKASL 66
           ++   ++G      H T A IGVC G +G++LP Q EVV LY   NI RMRLYDPN A+L
Sbjct: 21  LLGQLVVGSLATKQH-TGAPIGVCNGMVGDDLPPQAEVVALYKTNNIPRMRLYDPNPAAL 80

Query: 67  DALRGSNIELMLGLPNTDLQRIASSQAEANTWVQNNVKNFANVRFKYIAVGNEVQPSSST 126
           +ALRGSNI+L+LG+PN +LQ IA SQA AN WVQNNV+N+ANV+FKYIAVGNEV+PS S 
Sbjct: 81  EALRGSNIKLLLGVPNENLQYIALSQANANAWVQNNVRNYANVKFKYIAVGNEVKPSDSF 140

Query: 127 AQFLVPAMRNIQTAVSNAGLADRIKVSTAVDTSIIGVSFPPSRGAFKNEVMPLMNSIISF 186
           AQFLVPAMRNIQ A+S AGLA +IKVSTA+DT ++G +FPPS G+FK+E   L+  II F
Sbjct: 141 AQFLVPAMRNIQEAISLAGLAKKIKVSTAIDTGVLGETFPPSIGSFKSEYNALLYPIIRF 200

Query: 187 LVANRSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVTDGQFRYQNLFDAILDAVYAA 246
           LV+++SPLLVNLYPYF+Y GN +DIRLDYALFTAPS VV DG F Y+NLFDA+LD VYAA
Sbjct: 201 LVSHQSPLLVNLYPYFAYSGNTQDIRLDYALFTAPSVVVQDGNFGYRNLFDAMLDGVYAA 260

Query: 247 LEKVGGANINIVISESGWPSAGGTATSIDNARTYINNLIQHVKNGTPRRPQRPIETYIFG 306
           LEK GG ++ +VISE+GWPSA GTAT+IDNART+I+NLIQHVK GTPRRP RPIETYIF 
Sbjct: 261 LEKAGGGSLKVVISETGWPSAAGTATTIDNARTFISNLIQHVKEGTPRRPGRPIETYIFA 320

Query: 307 MFDENQKNPELEKHFGLFFPNKQPKFPVNFN 338
           MFDEN+K PELEKH+GLF P KQPK+ ++FN
Sbjct: 321 MFDENRKTPELEKHWGLFSPTKQPKYQISFN 350

BLAST of CmUC03G060470 vs. ExPASy Swiss-Prot
Match: P07979 (Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3)

HSP 1 Score: 424.5 bits (1090), Expect = 1.1e-117
Identity = 208/333 (62.46%), Postives = 263/333 (78.98%), Query Frame = 0

Query: 9   SLCLIGLFLPNLHLTSAQ-IGVCYGQMGNNLPTQREVVDLYNQRNIRRMRLYDPNKASLD 68
           ++ L+GL + +  +  AQ +GVCYG +GNNLP   +VV LY  +NIRRMRLYDPN+A+L 
Sbjct: 14  AIILLGLLVSSTEIVGAQSVGVCYGMLGNNLPPASQVVQLYKSKNIRRMRLYDPNQAALQ 73

Query: 69  ALRGSNIELMLGLPNTDLQRIASSQAEANTWVQNNVKNF-ANVRFKYIAVGNEVQP---S 128
           ALRGSNIE+MLG+PN+DLQ IA++ + AN WVQ NV+NF   V+F+YIAVGNEV P   +
Sbjct: 74  ALRGSNIEVMLGVPNSDLQNIAANPSNANNWVQRNVRNFWPAVKFRYIAVGNEVSPVTGT 133

Query: 129 SSTAQFLVPAMRNIQTAVSNAGLADRIKVSTAVDTSIIGVSFPPSRGAFKNEVMPLMNSI 188
           SS  ++L+PAMRNI+ A+S+AGL + IKVS++VD ++IG SFPPS+G+F+N+V   ++ I
Sbjct: 134 SSLTRYLLPAMRNIRNAISSAGLQNNIKVSSSVDMTLIGNSFPPSQGSFRNDVRSFIDPI 193

Query: 189 ISFLVANRSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVTDGQFRYQNLFDAILDAV 248
           I F+    SPLLVN+YPYFSY GNPRDI L YALFTAP+ VV DG   Y+NLFDA+ DAV
Sbjct: 194 IGFVRRINSPLLVNIYPYFSYAGNPRDISLPYALFTAPNVVVQDGSLGYRNLFDAMSDAV 253

Query: 249 YAALEKVGGANINIVISESGWPSAGGTATSIDNARTYINNLIQHVKNGTPRRPQRPIETY 308
           YAAL + GG +I IV+SESGWPSAG  A + +NA TY  NLIQHVK G+PRRP + IETY
Sbjct: 254 YAALSRAGGGSIEIVVSESGWPSAGAFAATTNNAATYYKNLIQHVKRGSPRRPNKVIETY 313

Query: 309 IFGMFDENQKNPELEKHFGLFFPNKQPKFPVNF 337
           +F MFDEN KNPELEKHFGLF PNKQPK+P++F
Sbjct: 314 LFAMFDENNKNPELEKHFGLFSPNKQPKYPLSF 346

BLAST of CmUC03G060470 vs. ExPASy Swiss-Prot
Match: Q03773 (Glucan endo-1,3-beta-glucosidase OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 7.4e-117
Identity = 203/318 (63.84%), Postives = 257/318 (80.82%), Query Frame = 0

Query: 23  TSAQIGVCYGQMGNNLPTQREVVDLYNQRNIRRMRLYDPNKASLDALRGSNIELMLGLPN 82
           T AQ GVCYG++GNNLPT +EVV LYNQ NIRRMR+Y P+   L+ALRGSNIEL+L +PN
Sbjct: 30  TDAQSGVCYGRLGNNLPTPQEVVALYNQANIRRMRIYGPSPEVLEALRGSNIELLLDIPN 89

Query: 83  TDLQRIASSQAEANTWVQNNVKNFA-NVRFKYIAVGNEVQPSSSTAQFLVPAMRNIQTAV 142
            +L+ +ASSQ  AN WVQ+N+KN+A NVRF+Y++VGNEV+P  S AQFLVPA+ NIQ A+
Sbjct: 90  DNLRNLASSQDNANKWVQDNIKNYANNVRFRYVSVGNEVKPEHSFAQFLVPALENIQRAI 149

Query: 143 SNAGLADRIKVSTAVDTSIIGVSFPPSRGAFKNEVM-PLMNSIISFLVANRSPLLVNLYP 202
           SNAGL +++KVSTA+DT  +  SFPPS+G+FK++     ++ +I FLV N +PL+VN+Y 
Sbjct: 150 SNAGLGNQVKVSTAIDTGALAESFPPSKGSFKSDYRGAYLDGVIRFLVNNNAPLMVNVYS 209

Query: 203 YFSYIGNPRDIRLDYALFTAPSTVVTDGQFRYQNLFDAILDAVYAALEKVGGANINIVIS 262
           YF+Y  NP+DI LDYALF +PS VV DG   Y+NLFDA +DAVYAALEK GG ++NIV+S
Sbjct: 210 YFAYTANPKDISLDYALFRSPSVVVQDGSLGYRNLFDASVDAVYAALEKAGGGSLNIVVS 269

Query: 263 ESGWPSAGGTATSIDNARTYINNLIQHVKNGTPRRPQRPIETYIFGMFDENQKNPELEKH 322
           ESGWPS+GGTATS+DNARTY  NL+++VK GTP+RP  P+ETY+F MFDENQK PE EK 
Sbjct: 270 ESGWPSSGGTATSLDNARTYNTNLVRNVKQGTPKRPGAPLETYVFAMFDENQKQPEFEKF 329

Query: 323 FGLFFP-NKQPKFPVNFN 338
           +GLF P  KQPK+ +NFN
Sbjct: 330 WGLFSPITKQPKYSINFN 347

BLAST of CmUC03G060470 vs. ExPASy Swiss-Prot
Match: F4J270 (Probable glucan endo-1,3-beta-glucosidase BG3 OS=Arabidopsis thaliana OX=3702 GN=BG3 PE=2 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 3.7e-116
Identity = 218/330 (66.06%), Postives = 256/330 (77.58%), Query Frame = 0

Query: 10  LCLIGLFLPNLHLTS-AQIGVCYGQMGNNLPTQREVVDLYNQRNIRRMRLYDPNKASLDA 69
           L L+   L +   T+  QIGVCYG+ GNNL    EVV LY QRNIRRMRLYDPN+ +L+A
Sbjct: 17  LLLLSFILASFFDTAVGQIGVCYGRNGNNLRPASEVVALYQQRNIRRMRLYDPNQETLNA 76

Query: 70  LRGSNIELMLGLPNTDLQRIASSQAEANTWVQNNVKNFANVRFKYIAVGNEVQPSSSTAQ 129
           LRGSNIEL+L +PN DLQR+ASSQAEA+TWV+NNV+N+ANV F+YI+VGNEVQPS   A 
Sbjct: 77  LRGSNIELVLDVPNPDLQRLASSQAEADTWVRNNVRNYANVTFRYISVGNEVQPSDQAAS 136

Query: 130 FLVPAMRNIQTAVSNAGLADRIKVSTAVDTSIIGVSFPPSRGAFKNEVMPLMNSIISFLV 189
           F++PAM+NI+ AVS+ G    IKVSTA+DT  I   FPPS G F  E    +  +ISFL 
Sbjct: 137 FVLPAMQNIERAVSSLG----IKVSTAIDTRGIS-GFPPSSGTFTPEFRSFIAPVISFLS 196

Query: 190 ANRSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVTDGQFRYQNLFDAILDAVYAALE 249
           + +SPLLVN YPYFSY GN RDIRLDY LFTAPSTVV DGQ +Y+NLF AILD VYA+LE
Sbjct: 197 SKQSPLLVNNYPYFSYTGNMRDIRLDYTLFTAPSTVVNDGQNQYRNLFHAILDTVYASLE 256

Query: 250 KVGGANINIVISESGWPSAGGTATSIDNARTYINNLIQHVKNGTPRRPQRPIETYIFGMF 309
           K GG ++ IV+SESGWP+AGG AT +DNARTY+NNLIQ VKNG+PRRP R  ETYIF MF
Sbjct: 257 KAGGGSLEIVVSESGWPTAGGAATGVDNARTYVNNLIQTVKNGSPRRPGRATETYIFAMF 316

Query: 310 DENQK-NPELEKHFGLFFPNKQPKFPVNFN 338
           DEN K  PE EK +GLF PN QPK+ VNFN
Sbjct: 317 DENSKQGPETEKFWGLFLPNLQPKYVVNFN 341

BLAST of CmUC03G060470 vs. ExPASy Swiss-Prot
Match: P36401 (Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum OX=4097 PE=1 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 1.9e-112
Identity = 204/336 (60.71%), Postives = 262/336 (77.98%), Query Frame = 0

Query: 7   IISLCLIG-LFLPNLHLTSAQIGVCYGQMGNNLPTQREVVDLYNQRNIRRMRLYDPNKAS 66
           I++L L+  L L  L  T AQ GVCYG+ GN LP+  +VV L N+ NIRRMR+YDP++ +
Sbjct: 5   IVTLLLLSVLTLATLDFTGAQAGVCYGRQGNGLPSPADVVSLCNRNNIRRMRIYDPDQPT 64

Query: 67  LDALRGSNIELMLGLPNTDLQRIASSQAEANTWVQNNVKNFANVRFKYIAVGNEVQPSSS 126
           L+ALRGSNIELMLG+PN DL+ +A+SQA A+TWVQNNV+N+ NV+F+YIAVGNEV P + 
Sbjct: 65  LEALRGSNIELMLGVPNPDLENVAASQANADTWVQNNVRNYGNVKFRYIAVGNEVSPLNE 124

Query: 127 TAQF---LVPAMRNIQTAVSNAGLADRIKVSTAVDTSIIGVSFPPSRGAFKNEVMPLMNS 186
            +++   L+ AMRNIQTA+S AGL ++IKVSTA++T +   + PPS G FK++V   +  
Sbjct: 125 NSKYVPVLLNAMRNIQTAISGAGLGNQIKVSTAIETGLTTDTSPPSNGRFKDDVRQFIEP 184

Query: 187 IISFLVANRSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVTDGQFRYQNLFDAILDA 246
           II+FLV NR+PLLVNLYPYF+ I N  DI+L+YALFT+   VV D    Y+NLFDAILDA
Sbjct: 185 IINFLVTNRAPLLVNLYPYFA-IANNADIKLEYALFTSSEVVVNDNGRGYRNLFDAILDA 244

Query: 247 VYAALEKVGGANINIVISESGWPSAG-GTATSIDNARTYINNLIQHVKNGTPRRPQRPIE 306
            Y+ALEK  G+++ IV+SESGWPSAG G  TSIDNARTY NNLI HVK G+P+RP  PIE
Sbjct: 245 TYSALEKASGSSLEIVVSESGWPSAGAGQLTSIDNARTYNNNLISHVKGGSPKRPSGPIE 304

Query: 307 TYIFGMFDENQKNPELEKHFGLFFPNKQPKFPVNFN 338
           TY+F +FDE+QK+PE+EKHFGLF  N QPK+ ++FN
Sbjct: 305 TYVFALFDEDQKDPEIEKHFGLFSANMQPKYQISFN 339

BLAST of CmUC03G060470 vs. ExPASy TrEMBL
Match: A0A1S3B9H8 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487244 PE=3 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 2.0e-157
Identity = 283/339 (83.48%), Postives = 303/339 (89.38%), Query Frame = 0

Query: 1   MANFC-SIISLCLIGLFLPNLHLTSAQIGVCYGQMGNNLPTQREVVDLYNQRNIRRMRLY 60
           MANF  ++I LCL+GL + NLHLTSAQIGVCYGQMGNNLP QREVV LYNQ NI+RMRLY
Sbjct: 1   MANFSWTMICLCLLGLLVQNLHLTSAQIGVCYGQMGNNLPPQREVVTLYNQNNIKRMRLY 60

Query: 61  DPNKASLDALRGSNIELMLGLPNTDLQRIASSQAEANTWVQNNVKNFANVRFKYIAVGNE 120
           DPN+ SLDALRGS IELMLGLPN DLQRIASSQAEAN WVQNN+KN+ NVRFKYIAVGNE
Sbjct: 61  DPNRGSLDALRGSTIELMLGLPNPDLQRIASSQAEANAWVQNNIKNYQNVRFKYIAVGNE 120

Query: 121 VQPSSSTAQFLVPAMRNIQTAVSNAGLADRIKVSTAVDTSIIGVSFPPSRGAFKNEVMPL 180
           VQPSSS A F+VPAMRNIQTA++NAGL +RIKVSTA+DTSI+ +S+PPS G  KNE MP 
Sbjct: 121 VQPSSSAASFVVPAMRNIQTALNNAGLVNRIKVSTAIDTSILAMSYPPSSGTIKNEDMPF 180

Query: 181 MNSIISFLVANRSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVTDGQFRYQNLFDAI 240
           M+S+I FL  N SPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVV DGQFRYQNLFDAI
Sbjct: 181 MSSVIRFLKDNGSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQFRYQNLFDAI 240

Query: 241 LDAVYAALEKVGGANINIVISESGWPSAGGTATSIDNARTYINNLIQHVKNGTPRRP-QR 300
           LDAVYAALEKVGG N+ IVISESGWPSAGGTA SIDNARTYINNLIQHVK GTPRRP  R
Sbjct: 241 LDAVYAALEKVGGGNLEIVISESGWPSAGGTAASIDNARTYINNLIQHVKRGTPRRPGGR 300

Query: 301 PIETYIFGMFDENQKNPELEKHFGLFFPNKQPKFPVNFN 338
           PIETYIF MFDENQKNPE EKHFGLFFPNKQ K+PVNFN
Sbjct: 301 PIETYIFAMFDENQKNPEFEKHFGLFFPNKQSKYPVNFN 339

BLAST of CmUC03G060470 vs. ExPASy TrEMBL
Match: A0A0A0LXW2 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G660200 PE=3 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 2.1e-154
Identity = 276/337 (81.90%), Postives = 302/337 (89.61%), Query Frame = 0

Query: 1   MANFCSIISLCLIGLFLPNLHLTSAQIGVCYGQMGNNLPTQREVVDLYNQRNIRRMRLYD 60
           MANF  IISLCL+GL +PNLHLT+AQIGVCYGQMGNNLP Q EVV L+NQ NI+RMRLYD
Sbjct: 1   MANFSPIISLCLLGLLVPNLHLTNAQIGVCYGQMGNNLPPQTEVVTLFNQNNIKRMRLYD 60

Query: 61  PNKASLDALRGSNIELMLGLPNTDLQRIASSQAEANTWVQNNVKNFANVRFKYIAVGNEV 120
           PN+ SLDALRGS IELMLG+PN+DLQRIASSQ EAN WVQNNVKN+ NVRF+YIAVGNEV
Sbjct: 61  PNRGSLDALRGSPIELMLGVPNSDLQRIASSQTEANAWVQNNVKNYPNVRFRYIAVGNEV 120

Query: 121 QPSSSTAQFLVPAMRNIQTAVSNAGLADRIKVSTAVDTSIIGVSFPPSRGAFKNEVMPLM 180
           QPSSS A F+VPAM NIQTA++NAGL  +IKVSTAV TSI+  S+PPSRG  KNEVMPLM
Sbjct: 121 QPSSSAASFVVPAMVNIQTALNNAGLG-KIKVSTAVATSIMADSYPPSRGTIKNEVMPLM 180

Query: 181 NSIISFLVANRSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVTDGQFRYQNLFDAIL 240
           N II FL  NRSPLL+NLYPYFSYIGNPRDIRLDYALFTAPSTVV DGQ+ YQNLFDA+L
Sbjct: 181 NPIIRFLNNNRSPLLLNLYPYFSYIGNPRDIRLDYALFTAPSTVVNDGQYLYQNLFDAML 240

Query: 241 DAVYAALEKVGGANINIVISESGWPSAGGTATSIDNARTYINNLIQHVKNGTPRRPQRPI 300
           DA+YAALEKVGG N+ IVISESGWPSAGGTATSI+NARTYINNLIQHVK GTPRRP RP 
Sbjct: 241 DALYAALEKVGGGNLEIVISESGWPSAGGTATSINNARTYINNLIQHVKRGTPRRPGRPT 300

Query: 301 ETYIFGMFDENQKNPELEKHFGLFFPNKQPKFPVNFN 338
           ETYIF MFDEN+K+PELEKHFGLFFPNKQ K+P+NFN
Sbjct: 301 ETYIFAMFDENKKSPELEKHFGLFFPNKQSKYPINFN 336

BLAST of CmUC03G060470 vs. ExPASy TrEMBL
Match: A0A6J1H865 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461371 PE=3 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 5.1e-153
Identity = 270/337 (80.12%), Postives = 297/337 (88.13%), Query Frame = 0

Query: 1   MANFCSIISLCLIGLFLPNLHLTSAQIGVCYGQMGNNLPTQREVVDLYNQRNIRRMRLYD 60
           MAN  SI+SLCL+GL +P+LHLTSAQIGVCYGQMGNNLP  REVV LYNQ NIRRMRLY 
Sbjct: 1   MANSYSIVSLCLLGLLMPSLHLTSAQIGVCYGQMGNNLPPSREVVALYNQNNIRRMRLYG 60

Query: 61  PNKASLDALRGSNIELMLGLPNTDLQRIASSQAEANTWVQNNVKNFANVRFKYIAVGNEV 120
           PN+ SL AL GSNIEL+L +PN DL +IASSQAEANTWVQNN+++  NVRFKYIAVGNEV
Sbjct: 61  PNRDSLGALSGSNIELILDVPNPDLPKIASSQAEANTWVQNNIRSLPNVRFKYIAVGNEV 120

Query: 121 QPSSSTAQFLVPAMRNIQTAVSNAGLADRIKVSTAVDTSIIGVSFPPSRGAFKNEVMPLM 180
           Q SSS AQF+VPAMRNIQTA+++ GL  RIKVSTA+DT ++G SFPPS GAFK+E MPL+
Sbjct: 121 QSSSSNAQFVVPAMRNIQTALASVGLGGRIKVSTAIDTGVLGTSFPPSNGAFKSEAMPLL 180

Query: 181 NSIISFLVANRSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVTDGQFRYQNLFDAIL 240
           N II FL+ N SPLLVNLYPYFSYIGN RDIRLDYALFTAPSTVVTDGQFRYQNLFDAIL
Sbjct: 181 NPIIRFLIENGSPLLVNLYPYFSYIGNMRDIRLDYALFTAPSTVVTDGQFRYQNLFDAIL 240

Query: 241 DAVYAALEKVGGANINIVISESGWPSAGGTATSIDNARTYINNLIQHVKNGTPRRPQRPI 300
           DA YAALEK GG N+ IV+SESGWP+AGGTATSIDNARTYINNLIQHVK GTPRRP +PI
Sbjct: 241 DATYAALEKAGGRNVEIVVSESGWPTAGGTATSIDNARTYINNLIQHVKRGTPRRPGKPI 300

Query: 301 ETYIFGMFDENQKNPELEKHFGLFFPNKQPKFPVNFN 338
           ETYIF MFDENQKNPE EKHFGLF PNKQ K+P+NFN
Sbjct: 301 ETYIFAMFDENQKNPEFEKHFGLFSPNKQLKYPINFN 337

BLAST of CmUC03G060470 vs. ExPASy TrEMBL
Match: A0A6J1JHX8 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484757 PE=3 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 6.6e-153
Identity = 272/337 (80.71%), Postives = 295/337 (87.54%), Query Frame = 0

Query: 1   MANFCSIISLCLIGLFLPNLHLTSAQIGVCYGQMGNNLPTQREVVDLYNQRNIRRMRLYD 60
           MAN  SIISLCL+GL +  LHLTSAQIGVCYGQMGNNLP  REVV LYNQ NIRRMRLY 
Sbjct: 1   MANSYSIISLCLLGLLMSTLHLTSAQIGVCYGQMGNNLPPSREVVALYNQNNIRRMRLYG 60

Query: 61  PNKASLDALRGSNIELMLGLPNTDLQRIASSQAEANTWVQNNVKNFANVRFKYIAVGNEV 120
           PN+ SL AL GSNIEL+L +PN DLQRIASSQAEANTWVQNN+++F NVRFKYI+VGNEV
Sbjct: 61  PNRDSLGALSGSNIELILDVPNPDLQRIASSQAEANTWVQNNIRSFPNVRFKYISVGNEV 120

Query: 121 QPSSSTAQFLVPAMRNIQTAVSNAGLADRIKVSTAVDTSIIGVSFPPSRGAFKNEVMPLM 180
           QPSSS AQF+VPAMRNIQTA++N GL+ RIKVST++DT +IG SFPPS GAFK+E MPL+
Sbjct: 121 QPSSSNAQFVVPAMRNIQTALANVGLSSRIKVSTSIDTGVIGTSFPPSNGAFKSEAMPLL 180

Query: 181 NSIISFLVANRSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVTDGQFRYQNLFDAIL 240
           N II FL  N SPLLVNLYPYFSYIGN RDIRLDYALFTAPSTVVTDGQFRYQNLFDAIL
Sbjct: 181 NPIIRFLNENSSPLLVNLYPYFSYIGNMRDIRLDYALFTAPSTVVTDGQFRYQNLFDAIL 240

Query: 241 DAVYAALEKVGGANINIVISESGWPSAGGTATSIDNARTYINNLIQHVKNGTPRRPQRPI 300
           DA YAALEK GG N+ IV+SESGWP+AGG ATSI NARTYINNLIQHVK GTPRRP RPI
Sbjct: 241 DATYAALEKAGGRNLEIVVSESGWPTAGGAATSISNARTYINNLIQHVKRGTPRRPGRPI 300

Query: 301 ETYIFGMFDENQKNPELEKHFGLFFPNKQPKFPVNFN 338
           E YIF MFDENQKNPE EKHFGLF PNKQ K+P+NFN
Sbjct: 301 EAYIFAMFDENQKNPEFEKHFGLFTPNKQLKYPINFN 337

BLAST of CmUC03G060470 vs. ExPASy TrEMBL
Match: A0A6J1CAZ7 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111010014 PE=3 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 1.4e-150
Identity = 272/338 (80.47%), Postives = 294/338 (86.98%), Query Frame = 0

Query: 1   MANFCSIISLCLIGLFLPNLHLTSAQIGVCYGQMGNNLPTQREVVDLYNQRNIRRMRLYD 60
           M N  SIISLCL+   LPNLHLTSAQIGVCYGQ+GNNLP QREVV LYNQ NIRRMRLYD
Sbjct: 1   MDNLYSIISLCLLAFLLPNLHLTSAQIGVCYGQIGNNLPPQREVVALYNQNNIRRMRLYD 60

Query: 61  PNKASLDALRGSNIELMLGLPNTDLQRIASSQAEANTWVQNNVKNFANVRFKYIAVGNEV 120
           PN+ASLDAL+ SNIEL+LGLPN DLQRIASSQ EAN WVQNNV+NFANVRFKYIAVGNEV
Sbjct: 61  PNRASLDALKXSNIELILGLPNPDLQRIASSQGEANAWVQNNVRNFANVRFKYIAVGNEV 120

Query: 121 QPSS-STAQFLVPAMRNIQTAVSNAGLADRIKVSTAVDTSIIGVSFPPSRGAFKNEVMPL 180
             S    A+F+ PAM+NIQ A+S AGL  +IKVSTA+DT +IGVSFPPS GAFK +V   
Sbjct: 121 LSSDPGAARFVGPAMQNIQNAISAAGLGGQIKVSTAIDTRVIGVSFPPSAGAFKTDVRGF 180

Query: 181 MNSIISFLVANRSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVTDGQFRYQNLFDAI 240
           M+ +I FLVANRSPLL+NLYPYFSYIGN RDIRLDYALFTAPS VVTDGQFRYQNLFDAI
Sbjct: 181 MDPVIRFLVANRSPLLLNLYPYFSYIGNTRDIRLDYALFTAPSVVVTDGQFRYQNLFDAI 240

Query: 241 LDAVYAALEKVGGANINIVISESGWPSAGGTATSIDNARTYINNLIQHVKNGTPRRPQRP 300
           LD+ YAALEK GG N+ IVISESGWPSAGGTATSIDNARTY NNLIQHVK GTPRRP RP
Sbjct: 241 LDSFYAALEKAGGGNLEIVISESGWPSAGGTATSIDNARTYNNNLIQHVKRGTPRRPGRP 300

Query: 301 IETYIFGMFDENQKNPELEKHFGLFFPNKQPKFPVNFN 338
           IETYIFGMFDENQK+PE EKHFGLF PNKQ K+P+NFN
Sbjct: 301 IETYIFGMFDENQKSPEFEKHFGLFSPNKQRKYPINFN 338

BLAST of CmUC03G060470 vs. TAIR 10
Match: AT3G57240.1 (beta-1,3-glucanase 3 )

HSP 1 Score: 419.5 bits (1077), Expect = 2.6e-117
Identity = 218/330 (66.06%), Postives = 256/330 (77.58%), Query Frame = 0

Query: 10  LCLIGLFLPNLHLTS-AQIGVCYGQMGNNLPTQREVVDLYNQRNIRRMRLYDPNKASLDA 69
           L L+   L +   T+  QIGVCYG+ GNNL    EVV LY QRNIRRMRLYDPN+ +L+A
Sbjct: 17  LLLLSFILASFFDTAVGQIGVCYGRNGNNLRPASEVVALYQQRNIRRMRLYDPNQETLNA 76

Query: 70  LRGSNIELMLGLPNTDLQRIASSQAEANTWVQNNVKNFANVRFKYIAVGNEVQPSSSTAQ 129
           LRGSNIEL+L +PN DLQR+ASSQAEA+TWV+NNV+N+ANV F+YI+VGNEVQPS   A 
Sbjct: 77  LRGSNIELVLDVPNPDLQRLASSQAEADTWVRNNVRNYANVTFRYISVGNEVQPSDQAAS 136

Query: 130 FLVPAMRNIQTAVSNAGLADRIKVSTAVDTSIIGVSFPPSRGAFKNEVMPLMNSIISFLV 189
           F++PAM+NI+ AVS+ G    IKVSTA+DT  I   FPPS G F  E    +  +ISFL 
Sbjct: 137 FVLPAMQNIERAVSSLG----IKVSTAIDTRGIS-GFPPSSGTFTPEFRSFIAPVISFLS 196

Query: 190 ANRSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVTDGQFRYQNLFDAILDAVYAALE 249
           + +SPLLVN YPYFSY GN RDIRLDY LFTAPSTVV DGQ +Y+NLF AILD VYA+LE
Sbjct: 197 SKQSPLLVNNYPYFSYTGNMRDIRLDYTLFTAPSTVVNDGQNQYRNLFHAILDTVYASLE 256

Query: 250 KVGGANINIVISESGWPSAGGTATSIDNARTYINNLIQHVKNGTPRRPQRPIETYIFGMF 309
           K GG ++ IV+SESGWP+AGG AT +DNARTY+NNLIQ VKNG+PRRP R  ETYIF MF
Sbjct: 257 KAGGGSLEIVVSESGWPTAGGAATGVDNARTYVNNLIQTVKNGSPRRPGRATETYIFAMF 316

Query: 310 DENQK-NPELEKHFGLFFPNKQPKFPVNFN 338
           DEN K  PE EK +GLF PN QPK+ VNFN
Sbjct: 317 DENSKQGPETEKFWGLFLPNLQPKYVVNFN 341

BLAST of CmUC03G060470 vs. TAIR 10
Match: AT3G57270.1 (beta-1,3-glucanase 1 )

HSP 1 Score: 405.2 bits (1040), Expect = 5.1e-113
Identity = 202/328 (61.59%), Postives = 257/328 (78.35%), Query Frame = 0

Query: 12  LIGLFLPNLHLTSAQIGVCYGQMGNNLPTQREVVDLYNQRNIRRMRLYDPNKASLDALRG 71
           L+GLF  N + T  Q+GVCYG+ GNNLP+  E + L+ Q+NI+R+RLY P+   L ALRG
Sbjct: 12  LLGLFFVNTNPTGGQVGVCYGRNGNNLPSPAETIALFKQKNIQRVRLYSPDHDVLAALRG 71

Query: 72  SNIELMLGLPNTDLQRIASSQAEANTWVQNNVKNFAN-VRFKYIAVGNEVQPSSSTAQFL 131
           SNIE+ LGLPN+ LQ +ASSQ++AN WVQ  V N+AN VRF+YI+VGNEV+ S S AQFL
Sbjct: 72  SNIEVTLGLPNSYLQSVASSQSQANAWVQTYVMNYANGVRFRYISVGNEVKISDSYAQFL 131

Query: 132 VPAMRNIQTAVSNAGLADRIKVSTAVDTSIIGVSFPPSRGAFKNEVMPLMNSIISFLVAN 191
           VPAM NI  AV  AGL  RIKVST+VD  ++  S+PPS+G+F+ +VM +M  II FLV+ 
Sbjct: 132 VPAMENIDRAVLAAGLGGRIKVSTSVDMGVLRESYPPSKGSFRGDVMVVMEPIIRFLVSK 191

Query: 192 RSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVTDGQFRYQNLFDAILDAVYAALEKV 251
            SPLL+NLY YFSY GN   IRLDYALFTAPS +V+D    YQNLFDA+LDA+Y+ALEK 
Sbjct: 192 NSPLLLNLYTYFSYAGNVGQIRLDYALFTAPSGIVSDPPRSYQNLFDAMLDAMYSALEKS 251

Query: 252 GGANINIVISESGWPSAGGTATSIDNARTYINNLIQHVKNGTPRRPQRPIETYIFGMFDE 311
           GGA++ IV++E+GWP+ GGT T+I+NAR Y NNLI+HVKNGTP+RP + IETY+F ++DE
Sbjct: 252 GGASLEIVVAETGWPTGGGTDTNIENARIYNNNLIKHVKNGTPKRPGKEIETYLFAIYDE 311

Query: 312 NQK--NPELEKHFGLFFPNKQPKFPVNF 337
           NQK   P +EK +GLF+PNKQPK+ +NF
Sbjct: 312 NQKPTPPYVEKFWGLFYPNKQPKYDINF 339

BLAST of CmUC03G060470 vs. TAIR 10
Match: AT4G16260.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 381.7 bits (979), Expect = 6.0e-106
Identity = 191/331 (57.70%), Postives = 249/331 (75.23%), Query Frame = 0

Query: 12  LIGLFLPN-LHLTSAQ-IGVCYGQMGNNLPTQREVVDLYNQRNIRRMRLYDPNKASLDAL 71
           LI LF+   L+ TS + +GVCYG MGNNLP+Q + + L+ Q NIRR+RLYDPN+A+L+AL
Sbjct: 7   LIALFITTILNPTSGESVGVCYGMMGNNLPSQSDTIALFRQNNIRRVRLYDPNQAALNAL 66

Query: 72  RGSNIELMLGLPNTDLQRIASSQAEANTWVQNNVKN-FANVRFKYIAVGNEVQPSSSTAQ 131
           R + IE+++G+PNTDL R  ++ + A +W+QNNV N +  V FKYIAVGNEV PS+    
Sbjct: 67  RNTGIEVIIGVPNTDL-RSLTNPSSARSWLQNNVLNYYPAVSFKYIAVGNEVSPSNG-GD 126

Query: 132 FLVPAMRNIQTAVSNAGLADRIKVSTAVDTSIIGVSFPPSRGAFKNEVMPLMNSIISFLV 191
            ++PAMRN+  A+  A L DRIKVSTA+D ++IG SFPPS G F+ +V   ++ +I FL 
Sbjct: 127 VVLPAMRNVYDALRGANLQDRIKVSTAIDMTLIGNSFPPSSGEFRGDVRWYIDPVIGFLT 186

Query: 192 ANRSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVTDGQFRYQNLFDAILDAVYAALE 251
           +  S LL N+YPYFSY+ NPRDI L YALFT+PS VV DG   YQNLFDA+LD VY+A+E
Sbjct: 187 STNSALLANIYPYFSYVDNPRDISLSYALFTSPSVVVWDGSRGYQNLFDALLDVVYSAVE 246

Query: 252 KVGGANINIVISESGWPSAGGTATSIDNARTYINNLIQHVK--NGTPRRPQRPIETYIFG 311
           + GG ++ +V+SESGWPS GG A S DNAR +  NL   V+   GTP+RP R +ETY+F 
Sbjct: 247 RSGGGSLPVVVSESGWPSNGGNAASFDNARAFYTNLASRVRENRGTPKRPGRGVETYLFA 306

Query: 312 MFDENQKNPELEKHFGLFFPNKQPKFPVNFN 338
           MFDENQK+PE+EK+FGLFFPNKQPKFP+ F+
Sbjct: 307 MFDENQKSPEIEKNFGLFFPNKQPKFPITFS 335

BLAST of CmUC03G060470 vs. TAIR 10
Match: AT3G57260.1 (beta-1,3-glucanase 2 )

HSP 1 Score: 371.3 bits (952), Expect = 8.1e-103
Identity = 189/332 (56.93%), Postives = 250/332 (75.30%), Query Frame = 0

Query: 7   IISLCLIGLFLPNLHLTSAQIGVCYGQMGNNLPTQREVVDLYNQRNIRRMRLYDPNKASL 66
           ++SL +   F    + T+ QIGVCYG +G+ LP+  +VV LY Q+NI+RMRLY P+  +L
Sbjct: 16  LLSLVIASFF----NHTAGQIGVCYGMLGDTLPSPSDVVALYKQQNIQRMRLYGPDPGAL 75

Query: 67  DALRGSNIELMLGLPNTDLQRIASSQAEANTWVQNNVKNFAN-VRFKYIAVGNEVQPSSS 126
            ALRGS+IEL+L +P++DL+R+ASSQ EA+ WVQ NV+++ + VRF+YI VGNEV+P  S
Sbjct: 76  AALRGSDIELILDVPSSDLERLASSQTEADKWVQENVQSYRDGVRFRYINVGNEVKP--S 135

Query: 127 TAQFLVPAMRNIQTAVSNAGLADRIKVSTAVDTSIIGVSFPPSRGAFKNEVMPLMNSIIS 186
              FL+ AM+NI+ AVS AGL   +KVSTA+ T     + PPS+G F++E    +  +I 
Sbjct: 136 VGGFLLQAMQNIENAVSGAGL--EVKVSTAIATDTTTDTSPPSQGRFRDEYKSFLEPVIG 195

Query: 187 FLVANRSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVTDGQFRYQNLFDAILDAVYA 246
           FL + +SPLLVNLYPYFSY+G+  +I LDYALFTA STV  D  + YQNLFDA LD+VYA
Sbjct: 196 FLASKQSPLLVNLYPYFSYMGDTANIHLDYALFTAQSTVDNDPGYSYQNLFDANLDSVYA 255

Query: 247 ALEKVGGANINIVISESGWPSAGGTATSIDNARTYINNLIQHVKNGTPRRPQRPIETYIF 306
           ALEK GG ++ IV+SE+GWP+ G   TS++NA+TY+NNLIQHVKNG+PRRP + IETYIF
Sbjct: 256 ALEKSGGGSLEIVVSETGWPTEGAVGTSVENAKTYVNNLIQHVKNGSPRRPGKAIETYIF 315

Query: 307 GMFDENQKNPELEKHFGLFFPNKQPKFPVNFN 338
            MFDEN+K P  EK +GLF P++Q K+ VNFN
Sbjct: 316 AMFDENKKEPTYEKFWGLFHPDRQSKYEVNFN 339

BLAST of CmUC03G060470 vs. TAIR 10
Match: AT5G56590.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 249.6 bits (636), Expect = 3.6e-66
Identity = 134/345 (38.84%), Postives = 213/345 (61.74%), Query Frame = 0

Query: 3   NFCSIISLCLIGLFLPNLHLTSAQIGVCYGQMGNNLPTQREVVDLYNQRNIRRMRLYDPN 62
           +F  I S+ ++ L L   +    ++GVCYG+  ++LPT  +VV L  Q NI+ +R+YD N
Sbjct: 4   DFKLIFSISILLLLLDCCY--GGKVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYN 63

Query: 63  KASLDALRGSNIELMLGLPNTDLQRIASSQAEANTWVQNNV-KNFANVRFKYIAVGNEV- 122
              L A   ++IELM+G+PN+DL   + SQ+  +TW++N+V   +   +  YI VG E  
Sbjct: 64  SQVLKAFGNTSIELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAEST 123

Query: 123 -QPSSSTAQFLVPAMRNIQTAVSNAGLADRIKVSTAVDTSIIGVSFPPSRGAFKNEVMPL 182
             P  + + F+VPAM+N+ TA+   GL+ RIKVST +   I+  SFPPS GAF +     
Sbjct: 124 DDPHINASSFVVPAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYAYF 183

Query: 183 MNSIISFLVANRSPLLVNLYPYFSYIGNPRDIRLDYALFTAPSTVVTDGQ-FRYQNLFDA 242
           +  ++ FL  N+SP +++LYPY++Y  +P ++ LDY LF + S V+       Y+N+FDA
Sbjct: 184 LRPMLEFLAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDA 243

Query: 243 ILDAVYAALEKVGGANINIVISESGWPSAGG----TATSIDNARTYINNLIQHV--KNGT 302
            +DA+Y AL  +    I I+++E+GWP+ G      A S DNA TY +N+I+HV    GT
Sbjct: 244 QVDALYYALTALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGT 303

Query: 303 PRRPQRPIETYIFGMFDENQK-NPELEKHFGLFFPNKQPKFPVNF 337
           P +P   +  YIF +F+EN+K   + E+++GLF+P++   + ++F
Sbjct: 304 PAKPGEAMNVYIFSLFNENRKAGLDSERNWGLFYPDQTSVYQLDF 346

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038876764.18.9e-16083.38glucan endo-1,3-beta-glucosidase, basic isoform-like [Benincasa hispida][more]
XP_008443726.14.1e-15783.48PREDICTED: glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo][more]
XP_031737288.14.3e-15481.90glucan endo-1,3-beta-glucosidase, basic isoform [Cucumis sativus] >KGN66700.1 hy... [more]
XP_022960641.11.1e-15280.12glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucurbita moschata][more]
XP_022987104.11.4e-15280.71glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
P524089.3e-12868.58Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS... [more]
P079791.1e-11762.46Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3[more]
Q037737.4e-11763.84Glucan endo-1,3-beta-glucosidase OS=Glycine max OX=3847 PE=1 SV=1[more]
F4J2703.7e-11666.06Probable glucan endo-1,3-beta-glucosidase BG3 OS=Arabidopsis thaliana OX=3702 GN... [more]
P364011.9e-11260.71Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum OX=4... [more]
Match NameE-valueIdentityDescription
A0A1S3B9H82.0e-15783.48(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487244 PE=3 SV... [more]
A0A0A0LXW22.1e-15481.90(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G660200 PE=3... [more]
A0A6J1H8655.1e-15380.12(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461371 P... [more]
A0A6J1JHX86.6e-15380.71(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484757 PE=... [more]
A0A6J1CAZ71.4e-15080.47(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111010014 ... [more]
Match NameE-valueIdentityDescription
AT3G57240.12.6e-11766.06beta-1,3-glucanase 3 [more]
AT3G57270.15.1e-11361.59beta-1,3-glucanase 1 [more]
AT4G16260.16.0e-10657.70Glycosyl hydrolase superfamily protein [more]
AT3G57260.18.1e-10356.93beta-1,3-glucanase 2 [more]
AT5G56590.13.6e-6638.84O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 26..337
e-value: 9.5E-135
score: 450.6
NoneNo IPR availablePANTHERPTHR32227:SF138GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG3-RELATEDcoord: 22..333
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 27..336
e-value: 1.4E-122
score: 409.2
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 255..268
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 22..333
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 27..335

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmUC03G060470.1CmUC03G060470.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds