CmUC03G052970 (gene) Watermelon (USVL531) v1

Overview


Name	CmUC03G052970
Type	gene
Organism	Citrullus mucosospermus (Watermelon (USVL531) v1)
Description	High-affinity nitrate transporter
Location	CmU531Chr03: 3374363 .. 3375599 (+)
RNA-Seq Expression	CmUC03G052970
Synteny	CmUC03G052970

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCTTTTAATCAAGGGTTTCCTCAAATCACCAACTTGTGCTCTTTGACTTGTTCTATTCACCACCACTTCTATATAAACCAAACCCAAGTCCTTAGCTCTGTTATCAATATCATCAAATCATCATCAAATCTGAGATTCACTTCACTTTTTGTCTGCAACTTGGAAGGGTTAAATCAAATCAATATGGGTTCAAATGGGTTTCTCTTGGCTGCCTTGTTTCTGTCCTGTTTCTTGGGATTCTCTCATGGAAATGTCCATTTCTCTTCTCTCCCAAGAACTTTGGAGGTCACTGCTTCACCAAAACCTGGACAAGGTAAGATTTTTCACCTCCCCCATCTCTTGAAACTCAAAACCCTTGATTCTTCCATGGAGATCTTAATAAAAAATTCAAACTTTACCAATTTTTAACAACTGGGTTGCCTTTTTTTTTTTTTTTTTTTTCTCTGCAGTTCTTAAAGCAGGTGTTGATAAAATCAGTGTGACCTGGGTTTTAAATGGGACCGTCACAGCCGGGTCGGATTCGGGTTACAAGAACATCAAGGTGAAGCTCTGTTACGCGCCGGTGAGCCAAGTGGACCGAGCGTGGAGGAAAACAGAGGACGATCTATCAAAGGACAAGACTTGTCAATTTAACATGGTGGAAAAGCCGTACAATCCGGCGAACAAGACGGTTCAAAAGTTTGAGTGGACGGTGGAGCGTGACATTCCGATGGGGACTTACTTTGTCAGAGCCTACGTTTTAAACTCCGCCGGCGATGAAGTGGCGTACGGCCAAACCACTGATGATAAAAAAGGAACAAACTTGTTTCAAGTTGAATCCATCACTGGTCGTCATATCTCGCTTGATATTGCCTCTGTTTGCTTCAGTGCTTTCTCTGTGGTGTCGTTGTTCGGATTTTTCCTCATCGACAAGAGAAAGGCCAAGAAGGCGGCGGCGGCGGGGGGGCAGTAAGTTAAGAAAAAGGTGGGAACTTTTGCTCTGTTTTGAGTTTCCTTCTCAGCTTGTGACAATGGCGATCTGCTTGATCAAGAGTAGACTTTATGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTCTCTTGTACTGTAATTTTCTAGGGGAAAAAGATCACCTATGTTGAGAAAAAGAAGGGTATGGTATGATATGATATATGGTGGAAAATAAAGTGTACTAATGTGTTTAATATTGATGTGGAAATCTTAATGACAATATTTGAATATTTGGATTTTGGG

mRNA sequence

CCTTTTAATCAAGGGTTTCCTCAAATCACCAACTTGTGCTCTTTGACTTGTTCTATTCACCACCACTTCTATATAAACCAAACCCAAGTCCTTAGCTCTGTTATCAATATCATCAAATCATCATCAAATCTGAGATTCACTTCACTTTTTGTCTGCAACTTGGAAGGGTTAAATCAAATCAATATGGGTTCAAATGGGTTTCTCTTGGCTGCCTTGTTTCTGTCCTGTTTCTTGGGATTCTCTCATGGAAATGTCCATTTCTCTTCTCTCCCAAGAACTTTGGAGGTCACTGCTTCACCAAAACCTGGACAAGTTCTTAAAGCAGGTGTTGATAAAATCAGTGTGACCTGGGTTTTAAATGGGACCGTCACAGCCGGGTCGGATTCGGGTTACAAGAACATCAAGGTGAAGCTCTGTTACGCGCCGGTGAGCCAAGTGGACCGAGCGTGGAGGAAAACAGAGGACGATCTATCAAAGGACAAGACTTGTCAATTTAACATGGTGGAAAAGCCGTACAATCCGGCGAACAAGACGGTTCAAAAGTTTGAGTGGACGGTGGAGCGTGACATTCCGATGGGGACTTACTTTGTCAGAGCCTACGTTTTAAACTCCGCCGGCGATGAAGTGGCGTACGGCCAAACCACTGATGATAAAAAAGGAACAAACTTGTTTCAAGTTGAATCCATCACTGGTCGTCATATCTCGCTTGATATTGCCTCTGTTTGCTTCAGTGCTTTCTCTGTGGTGTCGTTGTTCGGATTTTTCCTCATCGACAAGAGAAAGGCCAAGAAGGCGGCGGCGGCGGGGGGGCAGTAAGTTAAGAAAAAGGTGGGAACTTTTGCTCTGTTTTGAGTTTCCTTCTCAGCTTGTGACAATGGCGATCTGCTTGATCAAGAGTAGACTTTATGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTCTCTTGTACTGTAATTTTCTAGGGGAAAAAGATCACCTATGTTGAGAAAAAGAAGGGTATGGTATGATATGATATATGGTGGAAAATAAAGTGTACTAATGTGTTTAATATTGATGTGGAAATCTTAATGACAATATTTGAATATTTGGATTTTGGG

Coding sequence (CDS)

ATGGGTTCAAATGGGTTTCTCTTGGCTGCCTTGTTTCTGTCCTGTTTCTTGGGATTCTCTCATGGAAATGTCCATTTCTCTTCTCTCCCAAGAACTTTGGAGGTCACTGCTTCACCAAAACCTGGACAAGTTCTTAAAGCAGGTGTTGATAAAATCAGTGTGACCTGGGTTTTAAATGGGACCGTCACAGCCGGGTCGGATTCGGGTTACAAGAACATCAAGGTGAAGCTCTGTTACGCGCCGGTGAGCCAAGTGGACCGAGCGTGGAGGAAAACAGAGGACGATCTATCAAAGGACAAGACTTGTCAATTTAACATGGTGGAAAAGCCGTACAATCCGGCGAACAAGACGGTTCAAAAGTTTGAGTGGACGGTGGAGCGTGACATTCCGATGGGGACTTACTTTGTCAGAGCCTACGTTTTAAACTCCGCCGGCGATGAAGTGGCGTACGGCCAAACCACTGATGATAAAAAAGGAACAAACTTGTTTCAAGTTGAATCCATCACTGGTCGTCATATCTCGCTTGATATTGCCTCTGTTTGCTTCAGTGCTTTCTCTGTGGTGTCGTTGTTCGGATTTTTCCTCATCGACAAGAGAAAGGCCAAGAAGGCGGCGGCGGCGGGGGGGCAGTAA

Protein sequence

MGSNGFLLAALFLSCFLGFSHGNVHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNGTVTAGSDSGYKNIKVKLCYAPVSQVDRAWRKTEDDLSKDKTCQFNMVEKPYNPANKTVQKFEWTVERDIPMGTYFVRAYVLNSAGDEVAYGQTTDDKKGTNLFQVESITGRHISLDIASVCFSAFSVVSLFGFFLIDKRKAKKAAAAGGQ

Homology

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BLAST of CmUC03G052970 vs. NCBI nr
Match: XP_008439011.1 (PREDICTED: high-affinity nitrate transporter 3.1-like [Cucumis melo] >KAA0055266.1 high-affinity nitrate transporter 3.1-like [Cucumis melo var. makuwa] >TYJ99190.1 high-affinity nitrate transporter 3.1-like [Cucumis melo var. makuwa])

HSP 1 Score: 380.6 bits (976), Expect = 9.0e-102
Identity = 186/207 (89.86%), Postives = 193/207 (93.24%), Query Frame = 0


Query: 1   MGSNGFLLAALFLSCFLGFSHGNVHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNG 60
           M SNGFLLAALFLSCFLGFS GN+H SSLPRTLEVTASPKPGQVLKAGVDKI VTWVLNG
Sbjct: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60

Query: 61  TVTAGSDSGYKNIKVKLCYAPVSQVDRAWRKTEDDLSKDKTCQFNMVEKPYNPANKTVQK 120
           TV AGSDS YKNIK KLCYAPVSQVDRAWRKTEDDL KDKTCQF++VEKPYNPANKT Q 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120

Query: 121 FEWTVERDIPMGTYFVRAYVLNSAGDEVAYGQTTDDKKGTNLFQVESITGRHISLDIASV 180
           FEWTVERD+P GT+FVRAYV NSAG EVAYGQTTDDKKGTNLFQ+ESITGRH+SLDIASV
Sbjct: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180

Query: 181 CFSAFSVVSLFGFFLIDKRKAKKAAAA 208
           CFSAFSVVSLFGFF IDKRKAKKAAAA
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAA 207

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BLAST of CmUC03G052970 vs. NCBI nr
Match: NP_001267619.1 (high-affinity nitrate transporter 3.1-like precursor [Cucumis sativus] >ACV33078.2 high-affinity nitrate transport system component [Cucumis sativus] >KGN64351.1 hypothetical protein Csa_013320 [Cucumis sativus])

HSP 1 Score: 379.0 bits (972), Expect = 2.6e-101
Identity = 187/207 (90.34%), Postives = 194/207 (93.72%), Query Frame = 0


Query: 1   MGSNGFLLAALFLSCFLGFSHGNVHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNG 60
           M SN FLLAALFLSCFLGFSHGN+HFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLN 
Sbjct: 1   MASNAFLLAALFLSCFLGFSHGNIHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNE 60

Query: 61  TVTAGSDSGYKNIKVKLCYAPVSQVDRAWRKTEDDLSKDKTCQFNMVEKPYNPANKTVQK 120
           TV AGSDS YKNIK KLCYAPVSQVDRAWRKTEDDL KDKTCQF++VEK YNPANKTVQ 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFSIVEKQYNPANKTVQS 120

Query: 121 FEWTVERDIPMGTYFVRAYVLNSAGDEVAYGQTTDDKKGTNLFQVESITGRHISLDIASV 180
           FEWTV+RDIP GT+FVRAYVLNSAG+EVAYGQTTD KKGTNLFQ+ESITGRHISLDIAS 
Sbjct: 121 FEWTVKRDIPTGTFFVRAYVLNSAGEEVAYGQTTDAKKGTNLFQIESITGRHISLDIASA 180

Query: 181 CFSAFSVVSLFGFFLIDKRKAKKAAAA 208
           CFSAFSVVSLFGFF IDKRKAKKAAAA
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAA 207

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BLAST of CmUC03G052970 vs. NCBI nr
Match: XP_038894835.1 (high-affinity nitrate transporter 3.1-like [Benincasa hispida])

HSP 1 Score: 372.9 bits (956), Expect = 1.9e-99
Identity = 183/207 (88.41%), Postives = 191/207 (92.27%), Query Frame = 0


Query: 1   MGSNGFLLAALFLSCFLGFSHGNVHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNG 60
           M SNGFLLA LFLSCFLGFSHGN+H SSL RTLEVTASPKPGQVLKAGVDKISVTW+  G
Sbjct: 1   MASNGFLLATLFLSCFLGFSHGNIHLSSLQRTLEVTASPKPGQVLKAGVDKISVTWIFVG 60

Query: 61  TVTAGSDSGYKNIKVKLCYAPVSQVDRAWRKTEDDLSKDKTCQFNMVEKPYNPANKTVQK 120
           T    SDS YKNIK KLCYAPVSQVDRAWRKTEDDLSKDKTCQF++VEKPYNPANKTVQK
Sbjct: 61  TDKVKSDSDYKNIKAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFDIVEKPYNPANKTVQK 120

Query: 121 FEWTVERDIPMGTYFVRAYVLNSAGDEVAYGQTTDDKKGTNLFQVESITGRHISLDIASV 180
           FEWTVERD+P  T+FVR YVLNSAGD+VAYGQTTDDKKGTNLFQ+ESITGRHISLDIASV
Sbjct: 121 FEWTVERDVPTATFFVRVYVLNSAGDKVAYGQTTDDKKGTNLFQIESITGRHISLDIASV 180

Query: 181 CFSAFSVVSLFGFFLIDKRKAKKAAAA 208
           CFSAFSVVSLFGFF IDKRKAKKAAAA
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAA 207

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BLAST of CmUC03G052970 vs. NCBI nr
Match: XP_022924070.1 (high-affinity nitrate transporter 3.1 [Cucurbita moschata])

HSP 1 Score: 361.3 bits (926), Expect = 5.6e-96
Identity = 176/208 (84.62%), Postives = 189/208 (90.87%), Query Frame = 0


Query: 1   MGSNGFLLAALFLSCFLGFSHGNVHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNG 60
           M +NGFLLAALFL CFLGFSHG++HFSSLPR+LEVTASPKPGQVLKAGVDKISVTWVLNG
Sbjct: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66

Query: 61  TVTAGSDSGYKNIKVKLCYAPVSQVDRAWRKTEDDLSKDKTCQFNMVEKPYNPANKTVQK 120
           TV +GSDS YK ++ KLCYAPVSQVDRAWRKTEDDLSKDKTCQF +V KPYNP NKTVQK
Sbjct: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126

Query: 121 FEWTVERDIPMGTYFVRAYVLNSAGDEVAYGQTTDDKKGTNLFQVESITGRHISLDIASV 180
           FEWTV RDIP GT+FVRAY  NSAGDEV YGQTTD+KKGTNLFQ+E+ITGRHISLDIASV
Sbjct: 127 FEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186

Query: 181 CFSAFSVVSLFGFFLIDKRKAKKAAAAG 209
           CFSAFSV+SL GFFL  KRKAKKA A G
Sbjct: 187 CFSAFSVLSLSGFFLAGKRKAKKAEAPG 214

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BLAST of CmUC03G052970 vs. NCBI nr
Match: XP_023520111.1 (high-affinity nitrate transporter 3.1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 360.5 bits (924), Expect = 9.6e-96
Identity = 175/208 (84.13%), Postives = 189/208 (90.87%), Query Frame = 0


Query: 1   MGSNGFLLAALFLSCFLGFSHGNVHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNG 60
           M +NGFLLAALFL CF GFSHG++HFSSLPR+LEVTASPKPGQVLKAGVDKISVTW+LNG
Sbjct: 7   MSANGFLLAALFLFCFFGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWILNG 66

Query: 61  TVTAGSDSGYKNIKVKLCYAPVSQVDRAWRKTEDDLSKDKTCQFNMVEKPYNPANKTVQK 120
           TV +GSDS YK ++ KLCYAPVSQVDRAWRKTEDDLSKDKTCQF +V KPYNPANKTVQK
Sbjct: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPANKTVQK 126

Query: 121 FEWTVERDIPMGTYFVRAYVLNSAGDEVAYGQTTDDKKGTNLFQVESITGRHISLDIASV 180
           FEWTV RDIP GT+FVRAY  NSAG+EV YGQTTD+KKGTNLFQ+ESITGRHISLDIASV
Sbjct: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIESITGRHISLDIASV 186

Query: 181 CFSAFSVVSLFGFFLIDKRKAKKAAAAG 209
           CFSAFSV+SL GFFL  KRKAKKA A G
Sbjct: 187 CFSAFSVLSLSGFFLAGKRKAKKAEAPG 214

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BLAST of CmUC03G052970 vs. ExPASy Swiss-Prot
Match: Q9FGS5 (High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana OX=3702 GN=NRT3.1 PE=1 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 2.6e-51
Identity = 110/209 (52.63%), Postives = 142/209 (67.94%), Query Frame = 0


Query: 1   MGSNGFLLAALFLSCFLGFSHGNVH---FSSLPR-TLEVTASPK---PGQVLKAGVDKIS 60
           M     L A+L +   +   HG      F  L +  L+VT  P    PG VL AG D ++
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 61  VTWVLNGTVTAGSDSGYKNIKVKLCYAPVSQVDRAWRKTEDDLSKDKTCQFNMVEKPYNP 120
           +TW L+ ++ +  ++ +K IKVKLCYAP SQVDR WRKT D+L KDKTC   ++ KPY  
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPY-- 120

Query: 121 ANKTVQKFEWTVERDIPMGTYFVRAYVLNSAGDEVAYGQTTDDKKGTNLFQVESITGRHI 180
            +KT+Q   WT+ERDIP GTYFVRAY +++ G EVAYGQ+TDD K TNLF V++I+GRH 
Sbjct: 121 -DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHA 180

Query: 181 SLDIASVCFSAFSVVSLFGFFLIDKRKAK 203
           SLDIAS+CFS FSVV+L  FF+ +KRKAK
Sbjct: 181 SLDIASICFSVFSVVALVVFFVNEKRKAK 205

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BLAST of CmUC03G052970 vs. ExPASy Swiss-Prot
Match: Q9SB67 (High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana OX=3702 GN=NRT3.2 PE=2 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 5.9e-48
Identity = 96/179 (53.63%), Postives = 129/179 (72.07%), Query Frame = 0


Query: 26  FSSLPRTLEVTASP-KPGQVLKAGVDKISVTWVL-NGTVTAGSDSGYKNIKVKLCYAPVS 85
           F+ L  ++EVTA P K   VL+AG D +++TW L + +    +D+ +K I+VKLCYAP+S
Sbjct: 29  FTDLQNSIEVTAKPVKDSGVLEAGKDMVTITWKLKSSSAKVDTDTAFKTIQVKLCYAPIS 88

Query: 86  QVDRAWRKTEDDLSKDKTCQFNMVEKPYNPANKTVQKFEWTVERDIPMGTYFVRAYVLNS 145
           QVDR WRKT++ L KD++C   +V K Y   +KT Q  +WT+  DIP GTYFVRAY ++ 
Sbjct: 89  QVDRPWRKTDNKLFKDRSCPHEIVSKAY---DKTPQSLDWTIGLDIPTGTYFVRAYGIDG 148

Query: 146 AGDEVAYGQTTDDKKGTNLFQVESITGRHISLDIASVCFSAFSVVSLFGFFLIDKRKAK 203
            G EVAYGQ+TD+ + TNLF V +I+G H+ LDIAS  FS FSVVSLF FF+++KRKAK
Sbjct: 149 DGHEVAYGQSTDEGRTTNLFSVHAISGHHVGLDIASTFFSVFSVVSLFVFFVMEKRKAK 204

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BLAST of CmUC03G052970 vs. ExPASy Swiss-Prot
Match: Q6ZI50 (High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. japonica OX=39947 GN=NAR2.1 PE=1 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 7.5e-43
Identity = 87/180 (48.33%), Postives = 119/180 (66.11%), Query Frame = 0


Query: 24  VHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNGTVTAGSDSGYKNIKVKLCYAPVS 83
           V  S LP+ L V  SPK G+V+ AG + ++VTW LN +  AG+D+ +K++KVKLCYAP S
Sbjct: 34  VFLSKLPKALVVGVSPKHGEVVHAGENTVTVTWSLNTSEPAGADAAFKSVKVKLCYAPAS 93

Query: 84  QVDRAWRKTEDDLSKDKTCQFNMVEKPYNPANKTVQKFEWTVERDIPMGTYFVRAYVLNS 143
           + DR WRK  DDL KDK CQF +  +PY        +F++ V RDIP  +YFVRAY +++
Sbjct: 94  RTDRGWRKASDDLHKDKACQFKVTVQPYAAG---AGRFDYVVARDIPTASYFVRAYAVDA 153

Query: 144 AGDEVAYGQTTDDKKGTNLFQVESITGRHISLDIASVCFSAFSVVSLFGFFLIDKRKAKK 203
           +G EVAYGQ++ D      F V  ITG H SL +A+  FS FS+ +L  FF+++KRK  K
Sbjct: 154 SGTEVAYGQSSPDA----AFDVAGITGIHASLKVAAGVFSTFSIAALAFFFVVEKRKKDK 206

[Close]

BLAST of CmUC03G052970 vs. ExPASy Swiss-Prot
Match: Q7XK12 (Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativa subsp. japonica OX=39947 GN=NAR2.2 PE=2 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 4.9e-42
Identity = 96/206 (46.60%), Postives = 124/206 (60.19%), Query Frame = 0


Query: 8   LAALFLSCFLGFSH-------GNVHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNG 67
           L  L L   LG  H            S+L + L V ASP+ GQVL AG D I+VTW LN 
Sbjct: 13  LPLLLLLVVLGACHVTPAAAAAGARLSALAKALVVEASPRAGQVLHAGEDAITVTWSLNA 72

Query: 68  T---VTAGSDSGYKNIKVKLCYAPVSQVDRAWRKTEDDLSKDKTCQFNMVEKPYNPANKT 127
           T     AG+D+GYK +KV LCYAP SQV R WRK  DDLSKDK CQF + ++PY+ A   
Sbjct: 73  TAAAAAAGADAGYKAVKVTLCYAPASQVGRGWRKAHDDLSKDKACQFKIAQQPYDGAG-- 132

Query: 128 VQKFEWTVERDIPMGTYFVRAYVLNSAGDEVAYGQTTDDKKGTNLFQVESITGRHISLDI 187
             KFE+TV RD+P  +Y+VRAY L+++G  VAYG+T         F V  ITG   S+++
Sbjct: 133 --KFEYTVARDVPTASYYVRAYALDASGARVAYGETAPSAS----FAVAGITGVTASIEV 192

Query: 188 ASVCFSAFSVVSLFGFFLIDKRKAKK 204
           A+   SAFSV +L  F +++ +K  K
Sbjct: 193 AAGVLSAFSVAALAVFLVLENKKKNK 210

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BLAST of CmUC03G052970 vs. ExPASy TrEMBL
Match: A0A5A7ULV7 (High-affinity nitrate transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004000 PE=3 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 4.3e-102
Identity = 186/207 (89.86%), Postives = 193/207 (93.24%), Query Frame = 0


Query: 1   MGSNGFLLAALFLSCFLGFSHGNVHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNG 60
           M SNGFLLAALFLSCFLGFS GN+H SSLPRTLEVTASPKPGQVLKAGVDKI VTWVLNG
Sbjct: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60

Query: 61  TVTAGSDSGYKNIKVKLCYAPVSQVDRAWRKTEDDLSKDKTCQFNMVEKPYNPANKTVQK 120
           TV AGSDS YKNIK KLCYAPVSQVDRAWRKTEDDL KDKTCQF++VEKPYNPANKT Q 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120

Query: 121 FEWTVERDIPMGTYFVRAYVLNSAGDEVAYGQTTDDKKGTNLFQVESITGRHISLDIASV 180
           FEWTVERD+P GT+FVRAYV NSAG EVAYGQTTDDKKGTNLFQ+ESITGRH+SLDIASV
Sbjct: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180

Query: 181 CFSAFSVVSLFGFFLIDKRKAKKAAAA 208
           CFSAFSVVSLFGFF IDKRKAKKAAAA
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAA 207

[Close]

BLAST of CmUC03G052970 vs. ExPASy TrEMBL
Match: A0A1S3AXC1 (High-affinity nitrate transporter OS=Cucumis melo OX=3656 GN=LOC103483931 PE=3 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 4.3e-102
Identity = 186/207 (89.86%), Postives = 193/207 (93.24%), Query Frame = 0


Query: 1   MGSNGFLLAALFLSCFLGFSHGNVHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNG 60
           M SNGFLLAALFLSCFLGFS GN+H SSLPRTLEVTASPKPGQVLKAGVDKI VTWVLNG
Sbjct: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60

Query: 61  TVTAGSDSGYKNIKVKLCYAPVSQVDRAWRKTEDDLSKDKTCQFNMVEKPYNPANKTVQK 120
           TV AGSDS YKNIK KLCYAPVSQVDRAWRKTEDDL KDKTCQF++VEKPYNPANKT Q 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120

Query: 121 FEWTVERDIPMGTYFVRAYVLNSAGDEVAYGQTTDDKKGTNLFQVESITGRHISLDIASV 180
           FEWTVERD+P GT+FVRAYV NSAG EVAYGQTTDDKKGTNLFQ+ESITGRH+SLDIASV
Sbjct: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180

Query: 181 CFSAFSVVSLFGFFLIDKRKAKKAAAA 208
           CFSAFSVVSLFGFF IDKRKAKKAAAA
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAA 207

[Close]

BLAST of CmUC03G052970 vs. ExPASy TrEMBL
Match: C8CHG8 (High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NAR2 PE=2 SV=2)

HSP 1 Score: 379.0 bits (972), Expect = 1.3e-101
Identity = 187/207 (90.34%), Postives = 194/207 (93.72%), Query Frame = 0


Query: 1   MGSNGFLLAALFLSCFLGFSHGNVHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNG 60
           M SN FLLAALFLSCFLGFSHGN+HFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLN 
Sbjct: 1   MASNAFLLAALFLSCFLGFSHGNIHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNE 60

Query: 61  TVTAGSDSGYKNIKVKLCYAPVSQVDRAWRKTEDDLSKDKTCQFNMVEKPYNPANKTVQK 120
           TV AGSDS YKNIK KLCYAPVSQVDRAWRKTEDDL KDKTCQF++VEK YNPANKTVQ 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFSIVEKQYNPANKTVQS 120

Query: 121 FEWTVERDIPMGTYFVRAYVLNSAGDEVAYGQTTDDKKGTNLFQVESITGRHISLDIASV 180
           FEWTV+RDIP GT+FVRAYVLNSAG+EVAYGQTTD KKGTNLFQ+ESITGRHISLDIAS 
Sbjct: 121 FEWTVKRDIPTGTFFVRAYVLNSAGEEVAYGQTTDAKKGTNLFQIESITGRHISLDIASA 180

Query: 181 CFSAFSVVSLFGFFLIDKRKAKKAAAA 208
           CFSAFSVVSLFGFF IDKRKAKKAAAA
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAA 207

[Close]

BLAST of CmUC03G052970 vs. ExPASy TrEMBL
Match: A0A6J1EDS1 (High-affinity nitrate transporter OS=Cucurbita moschata OX=3662 GN=LOC111431611 PE=3 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 2.7e-96
Identity = 176/208 (84.62%), Postives = 189/208 (90.87%), Query Frame = 0


Query: 1   MGSNGFLLAALFLSCFLGFSHGNVHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNG 60
           M +NGFLLAALFL CFLGFSHG++HFSSLPR+LEVTASPKPGQVLKAGVDKISVTWVLNG
Sbjct: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66

Query: 61  TVTAGSDSGYKNIKVKLCYAPVSQVDRAWRKTEDDLSKDKTCQFNMVEKPYNPANKTVQK 120
           TV +GSDS YK ++ KLCYAPVSQVDRAWRKTEDDLSKDKTCQF +V KPYNP NKTVQK
Sbjct: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126

Query: 121 FEWTVERDIPMGTYFVRAYVLNSAGDEVAYGQTTDDKKGTNLFQVESITGRHISLDIASV 180
           FEWTV RDIP GT+FVRAY  NSAGDEV YGQTTD+KKGTNLFQ+E+ITGRHISLDIASV
Sbjct: 127 FEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186

Query: 181 CFSAFSVVSLFGFFLIDKRKAKKAAAAG 209
           CFSAFSV+SL GFFL  KRKAKKA A G
Sbjct: 187 CFSAFSVLSLSGFFLAGKRKAKKAEAPG 214

[Close]

BLAST of CmUC03G052970 vs. ExPASy TrEMBL
Match: A0A6J1KMC9 (High-affinity nitrate transporter OS=Cucurbita maxima OX=3661 GN=LOC111495476 PE=3 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 6.7e-95
Identity = 173/208 (83.17%), Postives = 189/208 (90.87%), Query Frame = 0


Query: 1   MGSNGFLLAALFLSCFLGFSHGNVHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNG 60
           M +NGFLLAALFL CFLGFSHG++HFSSLPR+LEVTASPKPGQVLKAGVDKISVTWVLNG
Sbjct: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66

Query: 61  TVTAGSDSGYKNIKVKLCYAPVSQVDRAWRKTEDDLSKDKTCQFNMVEKPYNPANKTVQK 120
           TV +GSDS YK ++ KLCYAPVSQVDRAWRKTEDDLSKDKTCQF +V KPYNPANKTVQK
Sbjct: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPANKTVQK 126

Query: 121 FEWTVERDIPMGTYFVRAYVLNSAGDEVAYGQTTDDKKGTNLFQVESITGRHISLDIASV 180
           FEWTV RDIP GT+FVRAY  NSAG+EV YGQT+D+KKGTNLFQ+++ITGRHISLDIASV
Sbjct: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTSDNKKGTNLFQIKAITGRHISLDIASV 186

Query: 181 CFSAFSVVSLFGFFLIDKRKAKKAAAAG 209
           CFSAFSV+SL GFFL  KRK KKA A G
Sbjct: 187 CFSAFSVLSLSGFFLAGKRKTKKAEAPG 214

[Close]

BLAST of CmUC03G052970 vs. TAIR 10
Match: AT5G50200.3 (nitrate transmembrane transporters )

HSP 1 Score: 203.4 bits (516), Expect = 1.8e-52
Identity = 110/209 (52.63%), Postives = 142/209 (67.94%), Query Frame = 0


Query: 1   MGSNGFLLAALFLSCFLGFSHGNVH---FSSLPR-TLEVTASPK---PGQVLKAGVDKIS 60
           M     L A+L +   +   HG      F  L +  L+VT  P    PG VL AG D ++
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 61  VTWVLNGTVTAGSDSGYKNIKVKLCYAPVSQVDRAWRKTEDDLSKDKTCQFNMVEKPYNP 120
           +TW L+ ++ +  ++ +K IKVKLCYAP SQVDR WRKT D+L KDKTC   ++ KPY  
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPY-- 120

Query: 121 ANKTVQKFEWTVERDIPMGTYFVRAYVLNSAGDEVAYGQTTDDKKGTNLFQVESITGRHI 180
            +KT+Q   WT+ERDIP GTYFVRAY +++ G EVAYGQ+TDD K TNLF V++I+GRH 
Sbjct: 121 -DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHA 180

Query: 181 SLDIASVCFSAFSVVSLFGFFLIDKRKAK 203
           SLDIAS+CFS FSVV+L  FF+ +KRKAK
Sbjct: 181 SLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of CmUC03G052970 vs. TAIR 10
Match: AT5G50200.2 (nitrate transmembrane transporters )

HSP 1 Score: 203.4 bits (516), Expect = 1.8e-52
Identity = 110/209 (52.63%), Postives = 142/209 (67.94%), Query Frame = 0


Query: 1   MGSNGFLLAALFLSCFLGFSHGNVH---FSSLPR-TLEVTASPK---PGQVLKAGVDKIS 60
           M     L A+L +   +   HG      F  L +  L+VT  P    PG VL AG D ++
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 61  VTWVLNGTVTAGSDSGYKNIKVKLCYAPVSQVDRAWRKTEDDLSKDKTCQFNMVEKPYNP 120
           +TW L+ ++ +  ++ +K IKVKLCYAP SQVDR WRKT D+L KDKTC   ++ KPY  
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPY-- 120

Query: 121 ANKTVQKFEWTVERDIPMGTYFVRAYVLNSAGDEVAYGQTTDDKKGTNLFQVESITGRHI 180
            +KT+Q   WT+ERDIP GTYFVRAY +++ G EVAYGQ+TDD K TNLF V++I+GRH 
Sbjct: 121 -DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHA 180

Query: 181 SLDIASVCFSAFSVVSLFGFFLIDKRKAK 203
           SLDIAS+CFS FSVV+L  FF+ +KRKAK
Sbjct: 181 SLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of CmUC03G052970 vs. TAIR 10
Match: AT5G50200.1 (nitrate transmembrane transporters )

HSP 1 Score: 203.4 bits (516), Expect = 1.8e-52
Identity = 110/209 (52.63%), Postives = 142/209 (67.94%), Query Frame = 0


Query: 1   MGSNGFLLAALFLSCFLGFSHGNVH---FSSLPR-TLEVTASPK---PGQVLKAGVDKIS 60
           M     L A+L +   +   HG      F  L +  L+VT  P    PG VL AG D ++
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 61  VTWVLNGTVTAGSDSGYKNIKVKLCYAPVSQVDRAWRKTEDDLSKDKTCQFNMVEKPYNP 120
           +TW L+ ++ +  ++ +K IKVKLCYAP SQVDR WRKT D+L KDKTC   ++ KPY  
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPY-- 120

Query: 121 ANKTVQKFEWTVERDIPMGTYFVRAYVLNSAGDEVAYGQTTDDKKGTNLFQVESITGRHI 180
            +KT+Q   WT+ERDIP GTYFVRAY +++ G EVAYGQ+TDD K TNLF V++I+GRH 
Sbjct: 121 -DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHA 180

Query: 181 SLDIASVCFSAFSVVSLFGFFLIDKRKAK 203
           SLDIAS+CFS FSVV+L  FF+ +KRKAK
Sbjct: 181 SLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of CmUC03G052970 vs. TAIR 10
Match: AT4G24730.4 (Calcineurin-like metallo-phosphoesterase superfamily protein )

HSP 1 Score: 192.2 bits (487), Expect = 4.2e-49
Identity = 96/179 (53.63%), Postives = 129/179 (72.07%), Query Frame = 0


Query: 26  FSSLPRTLEVTASP-KPGQVLKAGVDKISVTWVL-NGTVTAGSDSGYKNIKVKLCYAPVS 85
           F+ L  ++EVTA P K   VL+AG D +++TW L + +    +D+ +K I+VKLCYAP+S
Sbjct: 51  FTDLQNSIEVTAKPVKDSGVLEAGKDMVTITWKLKSSSAKVDTDTAFKTIQVKLCYAPIS 110

Query: 86  QVDRAWRKTEDDLSKDKTCQFNMVEKPYNPANKTVQKFEWTVERDIPMGTYFVRAYVLNS 145
           QVDR WRKT++ L KD++C   +V K Y   +KT Q  +WT+  DIP GTYFVRAY ++ 
Sbjct: 111 QVDRPWRKTDNKLFKDRSCPHEIVSKAY---DKTPQSLDWTIGLDIPTGTYFVRAYGIDG 170

Query: 146 AGDEVAYGQTTDDKKGTNLFQVESITGRHISLDIASVCFSAFSVVSLFGFFLIDKRKAK 203
            G EVAYGQ+TD+ + TNLF V +I+G H+ LDIAS  FS FSVVSLF FF+++KRKAK
Sbjct: 171 DGHEVAYGQSTDEGRTTNLFSVHAISGHHVGLDIASTFFSVFSVVSLFVFFVMEKRKAK 226

[Close]

BLAST of CmUC03G052970 vs. TAIR 10
Match: AT4G24730.1 (Calcineurin-like metallo-phosphoesterase superfamily protein )

HSP 1 Score: 185.7 bits (470), Expect = 3.9e-47
Identity = 97/184 (52.72%), Postives = 127/184 (69.02%), Query Frame = 0


Query: 20  SHGNVHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVL-NGTVTAGSDSGYKNIKVKLC 79
           SHG VH  SL   LE         VL+AG D +++TW L + +    +D+ +K I+VKLC
Sbjct: 265 SHG-VHHRSLEAALECP------PVLEAGKDMVTITWKLKSSSAKVDTDTAFKTIQVKLC 324

Query: 80  YAPVSQVDRAWRKTEDDLSKDKTCQFNMVEKPYNPANKTVQKFEWTVERDIPMGTYFVRA 139
           YAP+SQVDR WRKT++ L KD++C   +V K Y   +KT Q  +WT+  DIP GTYFVRA
Sbjct: 325 YAPISQVDRPWRKTDNKLFKDRSCPHEIVSKAY---DKTPQSLDWTIGLDIPTGTYFVRA 384

Query: 140 YVLNSAGDEVAYGQTTDDKKGTNLFQVESITGRHISLDIASVCFSAFSVVSLFGFFLIDK 199
           Y ++  G EVAYGQ+TD+ + TNLF V +I+G H+ LDIAS  FS FSVVSLF FF+++K
Sbjct: 385 YGIDGDGHEVAYGQSTDEGRTTNLFSVHAISGHHVGLDIASTFFSVFSVVSLFVFFVMEK 438

Query: 200 RKAK 203
           RKAK
Sbjct: 445 RKAK 438

The following BLAST results are available for this feature:

BLAST of CmUC03G052970 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008439011.1	9.0e-102	89.86	PREDICTED: high-affinity nitrate transporter 3.1-like [Cucumis melo] >KAA0055266...	[more]
NP_001267619.1	2.6e-101	90.34	high-affinity nitrate transporter 3.1-like precursor [Cucumis sativus] >ACV33078...	[more]
XP_038894835.1	1.9e-99	88.41	high-affinity nitrate transporter 3.1-like [Benincasa hispida]	[more]
XP_022924070.1	5.6e-96	84.62	high-affinity nitrate transporter 3.1 [Cucurbita moschata]	[more]
XP_023520111.1	9.6e-96	84.13	high-affinity nitrate transporter 3.1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmUC03G052970 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FGS5	2.6e-51	52.63	High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana OX=3702 GN=NRT3.1 ...	[more]
Q9SB67	5.9e-48	53.63	High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana OX=3702 GN=NRT3.2 ...	[more]
Q6ZI50	7.5e-43	48.33	High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. ...	[more]
Q7XK12	4.9e-42	46.60	Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativ...	[more]

BLAST of CmUC03G052970 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7ULV7	4.3e-102	89.86	High-affinity nitrate transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3AXC1	4.3e-102	89.86	High-affinity nitrate transporter OS=Cucumis melo OX=3656 GN=LOC103483931 PE=3 S...	[more]
C8CHG8	1.3e-101	90.34	High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NAR2 PE=2 SV=2	[more]
A0A6J1EDS1	2.7e-96	84.62	High-affinity nitrate transporter OS=Cucurbita moschata OX=3662 GN=LOC111431611 ...	[more]
A0A6J1KMC9	6.7e-95	83.17	High-affinity nitrate transporter OS=Cucurbita maxima OX=3661 GN=LOC111495476 PE...	[more]

BLAST of CmUC03G052970 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G50200.3	1.8e-52	52.63	nitrate transmembrane transporters	[more]
AT5G50200.2	1.8e-52	52.63	nitrate transmembrane transporters	[more]
AT5G50200.1	1.8e-52	52.63	nitrate transmembrane transporters	[more]
AT4G24730.4	4.2e-49	53.63	Calcineurin-like metallo-phosphoesterase superfamily protein	[more]
AT4G24730.1	3.9e-47	52.72	Calcineurin-like metallo-phosphoesterase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016605	High-affinity nitrate transporter	PIRSF	PIRSF012939	NAR2	coord: 1..205 e-value: 1.1E-84 score: 280.6
IPR016605	High-affinity nitrate transporter	PFAM	PF16974	NAR2	coord: 26..201 e-value: 3.5E-78 score: 260.9
IPR016605	High-affinity nitrate transporter	PANTHER	PTHR34806	HIGH-AFFINITY NITRATE TRANSPORTER 3.2	coord: 5..204
None	No IPR available	PANTHER	PTHR34806:SF1	HIGH-AFFINITY NITRATE TRANSPORTER 3.2	coord: 5..204

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmUC03G052970.1	CmUC03G052970.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042128	nitrate assimilation
biological_process	GO:0015706	nitrate transport
biological_process	GO:0010167	response to nitrate
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0015112	nitrate transmembrane transporter activity