CmUC01G016370 (gene) Watermelon (USVL531) v1

Overview
NameCmUC01G016370
Typegene
OrganismCitrullus mucosospermus (Watermelon (USVL531) v1)
DescriptionCytochrome P450
LocationCmU531Chr01: 30009824 .. 30013575 (-)
RNA-Seq ExpressionCmUC01G016370
SyntenyCmUC01G016370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGCAAATGTCAGTGTCCTGGCGTGAATCTATATAAATAGCTCACCGTGGTCACGTCTTCGTCCTCAGTCCCAAATCAAAACAGAAATACACTACACAAATATTATGGAAAATTACTCTTTGCTTTGCCTTTTTCTCTCTCTTATCTCTCTCATCTTCGCTTTCAATCTCCGCCGTCGCAGAAACTTACCGCCCAGTCCGCTGTCTCTTCCCATCATCGGCCACCTCCATTACCTCAAAATCCCCGTCCACCGAACTTTCCAGAACCTCTCTGCAAAATACGGACCAGTAATCTCTCTTTGGTTCGGATCTCGCCTCGTCGTCGTCGTCTCGTCATTGGACGTGATGGAGGAGTGCTTCACTAAGAACGACATCGTTTTAGCCAACCGTCCTCGGTTGCTAGTCGGCAAATACATCGGCTACAACCACACTACGATGGTAGCGTCACCGTACGGCGAACACTGGAGGAACCTCCGCCGAATTGGAGCTATCGAGATCTTTTCGGCATCTCGCCTCAATAAATTAGCTGGAATCCGAAAGGACGAAGTGGAACGGTTACTGAGGAAGCTATCGAGGAATTCGTTGAATGGATTCTCGAAGGTACATATGCAATCGGCGATTTCGGAACTGACGTTCAACATATCGATGAGAATGGCGGCCGGAAAGAGGTATTTCGGTGAGGAAGTGACGGATGAAGAGGAAGCGAGACAGTTCGGAGATTTGATTAAACAGGTGGTGGCGTTGGGAGGAGTTTCGAATCCAGGAGATTTCATTCCGATGATAAATTGGATTCCGAACGGGTTCAAGAGGAAGGTTTCGGGACTTGCAAAGAGGACGGACGCGTTTTTGCAGGGATTGATCGAGGAACATCGGAGCAAGAAGGAAGAAGGAAGGAACACGATGATCGATCATTTGCTCTCTCTGCAAGAATCAGAGCCGGAGAACTATGGAGATCAAATTATCAAAGGAATTATTCTGGTAATCTCCATATTTTCTCACTCACTTTTCTTGAATTTCAAGATTTTGACGGATTTCAAATGAATTTAGTTCTTTGTCACTATAAAATAAATAATTATTTTAAAATTAAAAGAATCTTCTTAGTTTTGGTGCTACCTCAGCTCCATTTATTTATGTGCAGGTTAGAAATTATACAGTGAAAAACTGTCAAAAGAAAAAGAAAAACCATTTAAAAATAATTTTTCTTTTTTTTGTGTGATAAAAGCTATAGGAGTATGACAACCAATCATTGTACTTGAAACCATTTTCCTTCGAATTTCCTAGATTTTTTTTTTAAATAGATTATTTAATTACCTTTATCATATTCTAATTTTTAATTAATACATCATATTTAAAAATTAATATTAATAAATTCTTAACTTTCTTTTTCTAGTTATCATTTAAATACAAAATTTGAAATACATTTATTTTAAATCTAATTAACTGAAAAGTATTTCTCGAAATTCAAATCCCAGAATTTAAAATCTATTTTTTTTTTCTTTTCTTTTTATCCAAACAGGAGGTTCATGTTGCTTTTAACATTTTGAATCTCCATTGCGATTCGGTTACTTCCACGGTTGCGCACTTTATTATTCCATCTCTTTTGGATAATCTTCAAAGGAGAAAAAGAATATGTCAGAACCGCAATCAAGATTCCAGTGATGGCACTTGCGGGACCTAACGTCTCACGTGTAACGATAACATTTGCCATAAAAGTATTTTTTAAAATAAAATTTATAATTCTTTCCTGATTAGTAATAAAAAGTTTTCCTGCAAATAATTTTTTATTTTTATTTTTCACCTGCCAGTCTGCCACTGGCCATTTTCTTCCATTCTTTGGCGTCCAATCAAGTAGGGTCATGAAATGCATATTGAAATTTTAATTTTATGTATATATCCACAACATATCAATAAAGATTTATTAAATAAATAAATGGGGAAATTACAAAATCTATCATTGAAGTAAGATAATTAATAGTTGTGATTATACCATTAAACTTTTAATTGTAAAAATTGGACTCTTAACTTCTAGAAGTGGTAAACTTCAACCCTAAGCTTATAATAATTGTAGAAATTGAACACAAATGATAAAAATTGAATGCTCAAACTTATACATATGTTACATTTCATACGATTATGTGGTTTTAACACTTATATAAGTTTGAGAGTTTAATATTCAAAATTTAAATTTTAAGGGTATAATTGTAAGAGTGGTTTTTACAATTATGGTAATAGTTACAATATACCTCTAAATATAAATAAATTTATTAAAATGATTTAAATTAATAATTGACTTGCTTCTACTCTCAAACTTAGATTTGTAAATCTTATTACTAGCATTTTTATTTACATATATTGATATTAATATATTATATTTATAAGTATTTATAAAAGGTATTGGTGACAATTTTTTTATATTTTTTTCTAATTATGATTGGGTTAAATTATTGGAGAGAAAAAAAAGTGCATTCGAGGTTACATCTATATGTTTTTATCTTATCCAATTGTTCAATTATATATAATTTGTCAACATGTGATTGGACACCCATCTCCATGCAAACACATGACAATATGTGATTGGACATTTTTAATGTTTATAGCTTAAGATGCCCCAAATATTTTTCATAAATATTGTTATTATTAAGAGACATGTAACATCTAGTTCCTAACTTGTCAGGATTTGTTTTTGTTTTTGTTTTTGTTTTTTTTTTTTTCTCTCTTTTGTATGTGTTGGTGTCACATTGAAAAGGCATATAAATGTGCAGGTTTTGTTACTAGCAGGAACGGACACATCAGCTGTGACAATTGAATGGGCTCTAGCTCACTTACTCAACAACCCAGAGGTACTCAAAAAGGCTAGAGAAGAGCTAGACGCTCAAATCGGAGAAGATCGACTAGTGGAAGAATCAGACGTCCCCAAGTTACCATATCTTCAAGGAATCATCTCTGAGACTCTTCGGTTGAACCCAGCTGCTCCGATGCTAGTGCCACATCTCACCTCCGACGATTGCACAATCAGCGGCTACGACATACCACGTGACACAATTGTATTGGTTAATGCATGGGCCATCCACAGAGATCCTAATCTATGGGAAGAACCCACACTATTCAAACCAGAAAGACACCAAAAGTCATCAGAACTAGGTGAACATCAGGTCCACAAGTTGGTTCCATTTGGAGTTGGACGTCGAGCTTGCCCTGGGTCCGGCATGGCTCAACGAGTGGTGGGGTTGGCTCTAGCGGCCCTGATTCAGTGCTACGAATGGCAGAGGATTGGCGAAGAGAAAATAGACATGACGGAGGGACGAGGCGTCACTATGCCCAAGGCAGTGCCATTGGAAGTCATGTGCAAACCTCGTCGTATCATCCACAACCTTTTTAACTGAAAAAATGGTTGGATTTAAAGGTATTTTATTGTTTATATTTAATAATGGATGTAGTTTTGATTTATTTGTATTATTATATCTTAAAAAAGGATTAAAAAGTATATATTTGGAAGGGTGTATGTATCGTTGTTCTGTAAACTTGTTTTCTTGTATCATAGCATTGGAATAATATATATCTAGTTGTTCAGTCTCTGTACAAATAGTTATTCAATTTGAGTGTTTTAGTTCTAATTTGAACATGATTTCACGGTTAAATTATTTTAAGATCCCTGGTTTGATCTCCACTTTTGAAAATTTTGAATAAAAGAGAACTAAATGGCCACTATTTGATTTAGTGACACATAACAGATTCGATCATAACTGTACTATGG

mRNA sequence

TGCAAATGTCAGTGTCCTGGCGTGAATCTATATAAATAGCTCACCGTGGTCACGTCTTCGTCCTCAGTCCCAAATCAAAACAGAAATACACTACACAAATATTATGGAAAATTACTCTTTGCTTTGCCTTTTTCTCTCTCTTATCTCTCTCATCTTCGCTTTCAATCTCCGCCGTCGCAGAAACTTACCGCCCAGTCCGCTGTCTCTTCCCATCATCGGCCACCTCCATTACCTCAAAATCCCCGTCCACCGAACTTTCCAGAACCTCTCTGCAAAATACGGACCAGTAATCTCTCTTTGGTTCGGATCTCGCCTCGTCGTCGTCGTCTCGTCATTGGACGTGATGGAGGAGTGCTTCACTAAGAACGACATCGTTTTAGCCAACCGTCCTCGGTTGCTAGTCGGCAAATACATCGGCTACAACCACACTACGATGGTAGCGTCACCGTACGGCGAACACTGGAGGAACCTCCGCCGAATTGGAGCTATCGAGATCTTTTCGGCATCTCGCCTCAATAAATTAGCTGGAATCCGAAAGGACGAAGTGGAACGGTTACTGAGGAAGCTATCGAGGAATTCGTTGAATGGATTCTCGAAGGTACATATGCAATCGGCGATTTCGGAACTGACGTTCAACATATCGATGAGAATGGCGGCCGGAAAGAGGTATTTCGGTGAGGAAGTGACGGATGAAGAGGAAGCGAGACAGTTCGGAGATTTGATTAAACAGGTGGTGGCGTTGGGAGGAGTTTCGAATCCAGGAGATTTCATTCCGATGATAAATTGGATTCCGAACGGGTTCAAGAGGAAGGTTTCGGGACTTGCAAAGAGGACGGACGCGTTTTTGCAGGGATTGATCGAGGAACATCGGAGCAAGAAGGAAGAAGGAAGGAACACGATGATCGATCATTTGCTCTCTCTGCAAGAATCAGAGCCGGAGAACTATGGAGATCAAATTATCAAAGGAATTATTCTGCTTAAGATGCCCCAAATATTTTTCATAAATATTGTTATTATTAAGAGACATGTTTTGTTACTAGCAGGAACGGACACATCAGCTGTGACAATTGAATGGGCTCTAGCTCACTTACTCAACAACCCAGAGGTACTCAAAAAGGCTAGAGAAGAGCTAGACGCTCAAATCGGAGAAGATCGACTAGTGGAAGAATCAGACGTCCCCAAGTTACCATATCTTCAAGGAATCATCTCTGAGACTCTTCGGTTGAACCCAGCTGCTCCGATGCTAGTGCCACATCTCACCTCCGACGATTGCACAATCAGCGGCTACGACATACCACGTGACACAATTGTATTGGTTAATGCATGGGCCATCCACAGAGATCCTAATCTATGGGAAGAACCCACACTATTCAAACCAGAAAGACACCAAAAGTCATCAGAACTAGGTGAACATCAGGTCCACAAGTTGGTTCCATTTGGAGTTGGACGTCGAGCTTGCCCTGGGTCCGGCATGGCTCAACGAGTGGTGGGGTTGGCTCTAGCGGCCCTGATTCAGTGCTACGAATGGCAGAGGATTGGCGAAGAGAAAATAGACATGACGGAGGGACGAGGCGTCACTATGCCCAAGGCAGTGCCATTGGAAGTCATGTGCAAACCTCGTCGTATCATCCACAACCTTTTTAACTGAAAAAATGGTTGGATTTAAAGGTATTTTATTGTTTATATTTAATAATGGATGTAGTTTTGATTTATTTGTATTATTATATCTTAAAAAAGGATTAAAAAGTATATATTTGGAAGGGTGTATGTATCGTTGTTCTGTAAACTTGTTTTCTTGTATCATAGCATTGGAATAATATATATCTAGTTGTTCAGTCTCTGTACAAATAGTTATTCAATTTGAGTGTTTTAGTTCTAATTTGAACATGATTTCACGGTTAAATTATTTTAAGATCCCTGGTTTGATCTCCACTTTTGAAAATTTTGAATAAAAGAGAACTAAATGGCCACTATTTGATTTAGTGACACATAACAGATTCGATCATAACTGTACTATGG

Coding sequence (CDS)

ATGGAAAATTACTCTTTGCTTTGCCTTTTTCTCTCTCTTATCTCTCTCATCTTCGCTTTCAATCTCCGCCGTCGCAGAAACTTACCGCCCAGTCCGCTGTCTCTTCCCATCATCGGCCACCTCCATTACCTCAAAATCCCCGTCCACCGAACTTTCCAGAACCTCTCTGCAAAATACGGACCAGTAATCTCTCTTTGGTTCGGATCTCGCCTCGTCGTCGTCGTCTCGTCATTGGACGTGATGGAGGAGTGCTTCACTAAGAACGACATCGTTTTAGCCAACCGTCCTCGGTTGCTAGTCGGCAAATACATCGGCTACAACCACACTACGATGGTAGCGTCACCGTACGGCGAACACTGGAGGAACCTCCGCCGAATTGGAGCTATCGAGATCTTTTCGGCATCTCGCCTCAATAAATTAGCTGGAATCCGAAAGGACGAAGTGGAACGGTTACTGAGGAAGCTATCGAGGAATTCGTTGAATGGATTCTCGAAGGTACATATGCAATCGGCGATTTCGGAACTGACGTTCAACATATCGATGAGAATGGCGGCCGGAAAGAGGTATTTCGGTGAGGAAGTGACGGATGAAGAGGAAGCGAGACAGTTCGGAGATTTGATTAAACAGGTGGTGGCGTTGGGAGGAGTTTCGAATCCAGGAGATTTCATTCCGATGATAAATTGGATTCCGAACGGGTTCAAGAGGAAGGTTTCGGGACTTGCAAAGAGGACGGACGCGTTTTTGCAGGGATTGATCGAGGAACATCGGAGCAAGAAGGAAGAAGGAAGGAACACGATGATCGATCATTTGCTCTCTCTGCAAGAATCAGAGCCGGAGAACTATGGAGATCAAATTATCAAAGGAATTATTCTGCTTAAGATGCCCCAAATATTTTTCATAAATATTGTTATTATTAAGAGACATGTTTTGTTACTAGCAGGAACGGACACATCAGCTGTGACAATTGAATGGGCTCTAGCTCACTTACTCAACAACCCAGAGGTACTCAAAAAGGCTAGAGAAGAGCTAGACGCTCAAATCGGAGAAGATCGACTAGTGGAAGAATCAGACGTCCCCAAGTTACCATATCTTCAAGGAATCATCTCTGAGACTCTTCGGTTGAACCCAGCTGCTCCGATGCTAGTGCCACATCTCACCTCCGACGATTGCACAATCAGCGGCTACGACATACCACGTGACACAATTGTATTGGTTAATGCATGGGCCATCCACAGAGATCCTAATCTATGGGAAGAACCCACACTATTCAAACCAGAAAGACACCAAAAGTCATCAGAACTAGGTGAACATCAGGTCCACAAGTTGGTTCCATTTGGAGTTGGACGTCGAGCTTGCCCTGGGTCCGGCATGGCTCAACGAGTGGTGGGGTTGGCTCTAGCGGCCCTGATTCAGTGCTACGAATGGCAGAGGATTGGCGAAGAGAAAATAGACATGACGGAGGGACGAGGCGTCACTATGCCCAAGGCAGTGCCATTGGAAGTCATGTGCAAACCTCGTCGTATCATCCACAACCTTTTTAACTGA

Protein sequence

MENYSLLCLFLSLISLIFAFNLRRRRNLPPSPLSLPIIGHLHYLKIPVHRTFQNLSAKYGPVISLWFGSRLVVVVSSLDVMEECFTKNDIVLANRPRLLVGKYIGYNHTTMVASPYGEHWRNLRRIGAIEIFSASRLNKLAGIRKDEVERLLRKLSRNSLNGFSKVHMQSAISELTFNISMRMAAGKRYFGEEVTDEEEARQFGDLIKQVVALGGVSNPGDFIPMINWIPNGFKRKVSGLAKRTDAFLQGLIEEHRSKKEEGRNTMIDHLLSLQESEPENYGDQIIKGIILLKMPQIFFINIVIIKRHVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDAQIGEDRLVEESDVPKLPYLQGIISETLRLNPAAPMLVPHLTSDDCTISGYDIPRDTIVLVNAWAIHRDPNLWEEPTLFKPERHQKSSELGEHQVHKLVPFGVGRRACPGSGMAQRVVGLALAALIQCYEWQRIGEEKIDMTEGRGVTMPKAVPLEVMCKPRRIIHNLFN
Homology
BLAST of CmUC01G016370 vs. NCBI nr
Match: XP_038882511.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 906.0 bits (2340), Expect = 1.5e-259
Identity = 459/514 (89.30%), Postives = 476/514 (92.61%), Query Frame = 0

Query: 1   MENYSLLCLFLSLISLIFAFNLRRRRNLPPSPLSLPIIGHLHYLKIPVHRTFQNLSAKYG 60
           MENYSLL L LSLI L+FAFNLRRRRNLPPSP SLPIIGHLHYLKIPVHRTFQNLSAKYG
Sbjct: 1   MENYSLLFLSLSLIFLLFAFNLRRRRNLPPSPPSLPIIGHLHYLKIPVHRTFQNLSAKYG 60

Query: 61  PVISLWFGSRLVVVVSSLDVMEECFTKNDIVLANRPRLLVGKYIGYNHTTMVASPYGEHW 120
           PVISLWFGSRLVVVVSSL V+EECFTKNDIVLANRPRLLVGKYIGYNHTTMVASPYG+HW
Sbjct: 61  PVISLWFGSRLVVVVSSLTVVEECFTKNDIVLANRPRLLVGKYIGYNHTTMVASPYGDHW 120

Query: 121 RNLRRIGAIEIFSASRLNKLAGIRKDEVERLLRKLSRNSLNGFSKVHMQSAISELTFNIS 180
           RNLRRIGAIEIFSASRLNK AGIR DEV+RLLRKLSRNS++GFSKVHMQSAISELTFNIS
Sbjct: 121 RNLRRIGAIEIFSASRLNKFAGIRSDEVKRLLRKLSRNSMHGFSKVHMQSAISELTFNIS 180

Query: 181 MRMAAGKRYFGEEVTDEEEARQFGDLIKQVVALGGVSNPGDFIPMINWIPNGFKRKVSGL 240
           MRMAAGKRYFGEEVTDE EA+QF +LIKQVVALGGVSNPGDFIPM+NWIPNGFKRKVS L
Sbjct: 181 MRMAAGKRYFGEEVTDEVEAKQFRELIKQVVALGGVSNPGDFIPMMNWIPNGFKRKVSRL 240

Query: 241 AKRTDAFLQGLIEEHRSKKEEGRNTMIDHLLSLQESEPENYGDQIIKGIILLKMPQIFFI 300
           AKR DAFLQ LI+EHRSKKEEGRNTMIDHLLSLQESEPE YGDQIIKGIIL         
Sbjct: 241 AKRMDAFLQELIDEHRSKKEEGRNTMIDHLLSLQESEPEYYGDQIIKGIIL--------- 300

Query: 301 NIVIIKRHVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDAQIGEDRLVEESDVPK 360
                   VLLLAGTDTSAVTIEWALAHLLNNPEVLKKAR+ELDAQIGE+RLVEESDVPK
Sbjct: 301 --------VLLLAGTDTSAVTIEWALAHLLNNPEVLKKARQELDAQIGEERLVEESDVPK 360

Query: 361 LPYLQGIISETLRLNPAAPMLVPHLTSDDCTISGYDIPRDTIVLVNAWAIHRDPNLWEEP 420
           LPYLQGIISETLRLNPAAPMLVPHLTSDDCTISGY+IPRDTIVLVNAWAIHRDPN WE+P
Sbjct: 361 LPYLQGIISETLRLNPAAPMLVPHLTSDDCTISGYEIPRDTIVLVNAWAIHRDPNQWEDP 420

Query: 421 TLFKPERHQKSSELGEHQVHKLVPFGVGRRACPGSGMAQRVVGLALAALIQCYEWQRIGE 480
           TLFKPERHQK  EL +HQV KLVPFGVGRRACPGSGMAQRVVGLALA LIQCYEW+RIGE
Sbjct: 421 TLFKPERHQK-LELCDHQVPKLVPFGVGRRACPGSGMAQRVVGLALATLIQCYEWERIGE 480

Query: 481 EKIDMTEGRGVTMPKAVPLEVMCKPRRIIHNLFN 515
           EK+DM EGRGVTMPKAVPLE MCKPRRIIHNLFN
Sbjct: 481 EKVDMAEGRGVTMPKAVPLEAMCKPRRIIHNLFN 496

BLAST of CmUC01G016370 vs. NCBI nr
Match: XP_008440328.1 (PREDICTED: cytochrome P450 81E8 [Cucumis melo])

HSP 1 Score: 857.4 bits (2214), Expect = 6.1e-245
Identity = 432/515 (83.88%), Postives = 459/515 (89.13%), Query Frame = 0

Query: 1   MENYSLLCLFLSLISLIFAFNLR-RRRNLPPSPLSLPIIGHLHYLKIPVHRTFQNLSAKY 60
           ME YSLL LFLSLISL+FAFNLR  RRNLPPSP SLPIIGHLHYLKIP+HRT Q LSAKY
Sbjct: 1   MEIYSLLYLFLSLISLLFAFNLRSSRRNLPPSPPSLPIIGHLHYLKIPLHRTLQKLSAKY 60

Query: 61  GPVISLWFGSRLVVVVSSLDVMEECFTKNDIVLANRPRLLVGKYIGYNHTTMVASPYGEH 120
           GPVISLWFGSRLVVVVSSL  +EECFTKNDIVLANRPRLLVGK++GYNHTTMVASPYG+H
Sbjct: 61  GPVISLWFGSRLVVVVSSLPAVEECFTKNDIVLANRPRLLVGKHVGYNHTTMVASPYGDH 120

Query: 121 WRNLRRIGAIEIFSASRLNKLAGIRKDEVERLLRKLSRNSLNGFSKVHMQSAISELTFNI 180
           WRNLRRIGAIEIFSASRLNK AG RKDEVERLL+KLSRNS+NGFSKV MQSAI+ELTFNI
Sbjct: 121 WRNLRRIGAIEIFSASRLNKFAGTRKDEVERLLKKLSRNSMNGFSKVEMQSAIAELTFNI 180

Query: 181 SMRMAAGKRYFGEEVTDEEEARQFGDLIKQVVALGGVSNPGDFIPMINWIPNGFKRKVSG 240
           SMRMAAGKRYFGEEVTD EEARQ  +LIKQ+V+LGGVSNPGDFIP++NWIPNGF+RKVS 
Sbjct: 181 SMRMAAGKRYFGEEVTDVEEARQSRELIKQIVSLGGVSNPGDFIPLMNWIPNGFRRKVSR 240

Query: 241 LAKRTDAFLQGLIEEHRSKKEEGRNTMIDHLLSLQESEPENYGDQIIKGIILLKMPQIFF 300
           LAKR D FLQGLI+EHRS K E RNTMIDHLLSLQESEPE YGDQIIKGIIL        
Sbjct: 241 LAKRMDGFLQGLIDEHRSNKCEERNTMIDHLLSLQESEPEYYGDQIIKGIIL-------- 300

Query: 301 INIVIIKRHVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDAQIGEDRLVEESDVP 360
                    VLLLAGTDTSAVTIEWALAHLLNN EVLKKAREELD QIGE RLVEESD+ 
Sbjct: 301 ---------VLLLAGTDTSAVTIEWALAHLLNNLEVLKKAREELDTQIGEKRLVEESDIL 360

Query: 361 KLPYLQGIISETLRLNPAAPMLVPHLTSDDCTISGYDIPRDTIVLVNAWAIHRDPNLWEE 420
           KLPYLQGIISETLRLNPAAPMLVPHLTS+DCTISGY IPRDTIVLVNAWAIHR+PN WEE
Sbjct: 361 KLPYLQGIISETLRLNPAAPMLVPHLTSNDCTISGYKIPRDTIVLVNAWAIHREPNQWEE 420

Query: 421 PTLFKPERHQKSSELGEHQVHKLVPFGVGRRACPGSGMAQRVVGLALAALIQCYEWQRIG 480
           PTLFKPERHQKS     HQ+ KL+PFG+GRRACPGSGMAQRVVGL LAALIQCYEW+RIG
Sbjct: 421 PTLFKPERHQKSESSSHHQIPKLIPFGIGRRACPGSGMAQRVVGLTLAALIQCYEWERIG 480

Query: 481 EEKIDMTEGRGVTMPKAVPLEVMCKPRRIIHNLFN 515
           EEK+DM EGRGVTMPK +PLE MCKPR IIH++FN
Sbjct: 481 EEKVDMAEGRGVTMPKLLPLEAMCKPRPIIHHIFN 498

BLAST of CmUC01G016370 vs. NCBI nr
Match: XP_011657880.2 (cytochrome P450 81Q32 [Cucumis sativus] >KAE8647508.1 hypothetical protein Csa_003220 [Cucumis sativus])

HSP 1 Score: 856.3 bits (2211), Expect = 1.4e-244
Identity = 432/515 (83.88%), Postives = 460/515 (89.32%), Query Frame = 0

Query: 1   MENYSLLCLFLSLISLIFAFNLR-RRRNLPPSPLSLPIIGHLHYLKIPVHRTFQNLSAKY 60
           ME  SLL LFLSLISL+FAFNLR RRRNLPPSP SLPIIGHLHY+KIP+HRT   LSAKY
Sbjct: 1   MEISSLLYLFLSLISLLFAFNLRSRRRNLPPSPPSLPIIGHLHYIKIPLHRTLHKLSAKY 60

Query: 61  GPVISLWFGSRLVVVVSSLDVMEECFTKNDIVLANRPRLLVGKYIGYNHTTMVASPYGEH 120
           GPVISLWFGSRLVVVVSSL  +EECFTKNDIVLANRPRLLVGK++GYNHTTMVASPYG+H
Sbjct: 61  GPVISLWFGSRLVVVVSSLPAVEECFTKNDIVLANRPRLLVGKHVGYNHTTMVASPYGDH 120

Query: 121 WRNLRRIGAIEIFSASRLNKLAGIRKDEVERLLRKLSRNSLNGFSKVHMQSAISELTFNI 180
           WRNLRRIGAIEIFSASRLNK AG RKDEVERLL+KLSRNS+ GFSKV MQS+ISELTFNI
Sbjct: 121 WRNLRRIGAIEIFSASRLNKFAGTRKDEVERLLKKLSRNSMYGFSKVEMQSSISELTFNI 180

Query: 181 SMRMAAGKRYFGEEVTDEEEARQFGDLIKQVVALGGVSNPGDFIPMINWIPNGFKRKVSG 240
           SMRMAAGKRYFGEEV D EEARQ  +LIKQ+V++GGVSNPGDFIPM+NWIPNG+KRKVS 
Sbjct: 181 SMRMAAGKRYFGEEVRDVEEARQSRELIKQIVSMGGVSNPGDFIPMMNWIPNGYKRKVSR 240

Query: 241 LAKRTDAFLQGLIEEHRSKKEEGRNTMIDHLLSLQESEPENYGDQIIKGIILLKMPQIFF 300
           LAKR D FLQGLI+EHRS KEE RNTMIDHLLSLQE+EPE YGDQIIKGIIL        
Sbjct: 241 LAKRMDGFLQGLIDEHRSNKEEERNTMIDHLLSLQETEPEYYGDQIIKGIIL-------- 300

Query: 301 INIVIIKRHVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDAQIGEDRLVEESDVP 360
                    VLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELD QIGE RLVEESDV 
Sbjct: 301 ---------VLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDTQIGEKRLVEESDVS 360

Query: 361 KLPYLQGIISETLRLNPAAPMLVPHLTSDDCTISGYDIPRDTIVLVNAWAIHRDPNLWEE 420
           KLPYLQGIISETLRLNPAAPMLVPHLTS+DCTISGY IPRDTIVLVNAWAIHRDPN WEE
Sbjct: 361 KLPYLQGIISETLRLNPAAPMLVPHLTSNDCTISGYKIPRDTIVLVNAWAIHRDPNQWEE 420

Query: 421 PTLFKPERHQKSSELGEHQVHKLVPFGVGRRACPGSGMAQRVVGLALAALIQCYEWQRIG 480
           PTLFKPERHQKS  + +H + KL+PFGVGRRACPGSGMAQRVVGL LAALIQCYEW+RIG
Sbjct: 421 PTLFKPERHQKSESI-DHHISKLIPFGVGRRACPGSGMAQRVVGLTLAALIQCYEWERIG 480

Query: 481 EEKIDMTEGRGVTMPKAVPLEVMCKPRRIIHNLFN 515
           +EK+DM+EGRGVTMPK VPLE MCKPR IIHN+FN
Sbjct: 481 DEKVDMSEGRGVTMPKMVPLEAMCKPRPIIHNIFN 497

BLAST of CmUC01G016370 vs. NCBI nr
Match: XP_022978934.1 (cytochrome P450 81E8-like [Cucurbita maxima])

HSP 1 Score: 841.3 bits (2172), Expect = 4.5e-240
Identity = 418/514 (81.32%), Postives = 460/514 (89.49%), Query Frame = 0

Query: 1   MENYSLLCLFLSLISLIFAFNLRRRRNLPPSPLSLPIIGHLHYLKIPVHRTFQNLSAKYG 60
           ME+ SLL L LSLI L+FAF+ RRRRNLPP P SLPIIGHLHYLK+PVHRT Q LSAKYG
Sbjct: 1   MEDRSLLYLLLSLIFLLFAFSPRRRRNLPPGPPSLPIIGHLHYLKVPVHRTLQKLSAKYG 60

Query: 61  PVISLWFGSRLVVVVSSLDVMEECFTKNDIVLANRPRLLVGKYIGYNHTTMVASPYGEHW 120
           PVISLWFGSRLVVVVSS  V+EECFTKND+VLANRPRLL+GK++GYN+TTMVASPYG+HW
Sbjct: 61  PVISLWFGSRLVVVVSSSAVVEECFTKNDVVLANRPRLLLGKHVGYNYTTMVASPYGDHW 120

Query: 121 RNLRRIGAIEIFSASRLNKLAGIRKDEVERLLRKLSRNSLNGFSKVHMQSAISELTFNIS 180
           RNLRRIGAIEIFSASRLNK AGIR+DEVERLLRKLS+NSL+GFSKV MQS ISELTFNIS
Sbjct: 121 RNLRRIGAIEIFSASRLNKFAGIRRDEVERLLRKLSKNSLHGFSKVQMQSTISELTFNIS 180

Query: 181 MRMAAGKRYFGEEVTDEEEARQFGDLIKQVVALGGVSNPGDFIPMINWIPNGFKRKVSGL 240
           M+MAAGKRYFG++V DE+EARQF +LIKQ+V+LGGVSNPGDFIPM+NW PN F+RKVS L
Sbjct: 181 MKMAAGKRYFGDDVADEDEARQFRELIKQIVSLGGVSNPGDFIPMLNWFPNVFERKVSRL 240

Query: 241 AKRTDAFLQGLIEEHRSKKEEGRNTMIDHLLSLQESEPENYGDQIIKGIILLKMPQIFFI 300
           AKRTDAFLQGLI+EHRS KEE RNTMIDHLLSLQESEPE YGDQIIKGIIL         
Sbjct: 241 AKRTDAFLQGLIDEHRSNKEEERNTMIDHLLSLQESEPELYGDQIIKGIIL--------- 300

Query: 301 NIVIIKRHVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDAQIGEDRLVEESDVPK 360
                   VLLLAGTDTSAVT+EWAL+HLLNNPE+LKKAREELDAQIG++RLVEESDVPK
Sbjct: 301 --------VLLLAGTDTSAVTMEWALSHLLNNPEILKKAREELDAQIGQERLVEESDVPK 360

Query: 361 LPYLQGIISETLRLNPAAPMLVPHLTSDDCTISGYDIPRDTIVLVNAWAIHRDPNLWEEP 420
           LPYLQGIISETLRLNPAAP+L+PHLTSDDCTIS Y +PRDTIVLVNAWAIHRDP+ WE+P
Sbjct: 361 LPYLQGIISETLRLNPAAPLLLPHLTSDDCTISNYKVPRDTIVLVNAWAIHRDPSQWEDP 420

Query: 421 TLFKPERHQKSSELGEHQVHKLVPFGVGRRACPGSGMAQRVVGLALAALIQCYEWQRIGE 480
           T F PERH+KS   G+H  HK +PFG+GRR+CPGSGMAQRVVGLALAALIQCYEW+RIGE
Sbjct: 421 TRFNPERHRKSE--GDH-AHKFLPFGIGRRSCPGSGMAQRVVGLALAALIQCYEWERIGE 480

Query: 481 EKIDMTEGRGVTMPKAVPLEVMCKPRRIIHNLFN 515
           EK+DM EGRG+TMPK VPLE MCKPR IIHNLFN
Sbjct: 481 EKVDMAEGRGLTMPKVVPLEAMCKPRPIIHNLFN 494

BLAST of CmUC01G016370 vs. NCBI nr
Match: XP_023543193.1 (cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 841.3 bits (2172), Expect = 4.5e-240
Identity = 419/514 (81.52%), Postives = 459/514 (89.30%), Query Frame = 0

Query: 1   MENYSLLCLFLSLISLIFAFNLRRRRNLPPSPLSLPIIGHLHYLKIPVHRTFQNLSAKYG 60
           ME+ SLL L LSLI L+FA + RRRRNLPP P SLPIIGHLHYLK+PVHRT Q LSAKYG
Sbjct: 1   MEDRSLLYLLLSLIFLLFALSPRRRRNLPPGPPSLPIIGHLHYLKVPVHRTLQKLSAKYG 60

Query: 61  PVISLWFGSRLVVVVSSLDVMEECFTKNDIVLANRPRLLVGKYIGYNHTTMVASPYGEHW 120
           PVISLWFGSRLVVVVSS  V+EECFTKND+VLANRPRLLVGK++GYN+TTMVASPYG+HW
Sbjct: 61  PVISLWFGSRLVVVVSSSAVVEECFTKNDVVLANRPRLLVGKHVGYNYTTMVASPYGDHW 120

Query: 121 RNLRRIGAIEIFSASRLNKLAGIRKDEVERLLRKLSRNSLNGFSKVHMQSAISELTFNIS 180
           RNLRRIGAIEIFSASRLNK AGIR+DEVERLLRKLS+NSL+GFSKV MQS ISELTFNIS
Sbjct: 121 RNLRRIGAIEIFSASRLNKFAGIRRDEVERLLRKLSKNSLHGFSKVQMQSTISELTFNIS 180

Query: 181 MRMAAGKRYFGEEVTDEEEARQFGDLIKQVVALGGVSNPGDFIPMINWIPNGFKRKVSGL 240
           MRMAAGKRYFG++V DE+EARQF +LIKQ+V+LGGVSNPGDFIPM+NWIPN F+RKVS L
Sbjct: 181 MRMAAGKRYFGDDVADEDEARQFRELIKQIVSLGGVSNPGDFIPMLNWIPNVFERKVSRL 240

Query: 241 AKRTDAFLQGLIEEHRSKKEEGRNTMIDHLLSLQESEPENYGDQIIKGIILLKMPQIFFI 300
           AKRTDAFLQGLI+EHRS KEE RNTMIDHLLSLQ+SEPE YGDQIIKGIIL         
Sbjct: 241 AKRTDAFLQGLIDEHRSNKEEERNTMIDHLLSLQQSEPELYGDQIIKGIIL--------- 300

Query: 301 NIVIIKRHVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDAQIGEDRLVEESDVPK 360
                   VLLLAGTDTSAVT+EWAL+HLLNNPE+LKKAREELDAQIG++RLVEESDVPK
Sbjct: 301 --------VLLLAGTDTSAVTMEWALSHLLNNPEILKKAREELDAQIGQERLVEESDVPK 360

Query: 361 LPYLQGIISETLRLNPAAPMLVPHLTSDDCTISGYDIPRDTIVLVNAWAIHRDPNLWEEP 420
           LPYLQGIISETLRL PAAP+LVPHLTSDDCTIS Y +PRDTIVLVNAWAIHRDP+ WE+P
Sbjct: 361 LPYLQGIISETLRLTPAAPLLVPHLTSDDCTISNYKVPRDTIVLVNAWAIHRDPSQWEDP 420

Query: 421 TLFKPERHQKSSELGEHQVHKLVPFGVGRRACPGSGMAQRVVGLALAALIQCYEWQRIGE 480
           T F PERH+KS   G+H  HK +PFG+GRR+CPGSGMAQRVVGLALAALIQCYEW+RIGE
Sbjct: 421 TRFNPERHRKSE--GDH-AHKFLPFGIGRRSCPGSGMAQRVVGLALAALIQCYEWERIGE 480

Query: 481 EKIDMTEGRGVTMPKAVPLEVMCKPRRIIHNLFN 515
           EK+DM EGRG+TMPK VPLE MCKPR IIHNLFN
Sbjct: 481 EKVDMAEGRGITMPKVVPLEAMCKPRPIIHNLFN 494

BLAST of CmUC01G016370 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 3.2e-172
Identity = 311/518 (60.04%), Postives = 386/518 (74.52%), Query Frame = 0

Query: 1   MENYSLLCLFLSLISLIFAFNL----RRRRNLPPSP-LSLPIIGHLHYLKIPVHRTFQNL 60
           ME+ +LL  FL+++ L  +  L    RRRRNLPPSP L+LP+IGHLH +   +HR+  +L
Sbjct: 1   MESSTLLYTFLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDL 60

Query: 61  SAKYGPVISLWFGSRLVVVVSSLDVMEECFTKNDIVLANRPRLLVGKYIGYNHTTMVASP 120
           S KYG V SL  G+RLV+VVSS    EECFTKNDIV ANRP  ++GKYIGYN+TTMV SP
Sbjct: 61  SKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSP 120

Query: 121 YGEHWRNLRRIGAIEIFSASRLNKLAGIRKDEVERLLRKLSRNSLNGFSKVHMQSAISEL 180
           YGEHWRNLRR+ A+EIFSA  LN+   IR+DEV++LL  L ++S   F KV M+S +SEL
Sbjct: 121 YGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSEL 180

Query: 181 TFNISMRMAAGKRYFGEEVTDEEEARQFGDLIKQVVALGGVSNPGDFIPMINWIP-NGFK 240
           +FN++MRM AGKRYFG++V D +EA+ F  LI +V    G SNPGDF+P + WI    ++
Sbjct: 181 SFNVTMRMVAGKRYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYE 240

Query: 241 RKVSGLAKRTDAFLQGLIEEHRSKKEEGRNTMIDHLLSLQESEPENYGDQIIKGIILLKM 300
           +KVS +++  DAFLQ LI  H S+  +   TMIDHLLSLQES+PE Y DQIIKGII+   
Sbjct: 241 KKVSKISQEMDAFLQRLI--HESRINKNNVTMIDHLLSLQESQPEYYTDQIIKGIIM--- 300

Query: 301 PQIFFINIVIIKRHVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDAQIGEDRLVE 360
                         VLLLAGTDTSAVT+EWA++ LLN+PE L+KAR E++ Q+G +RL+E
Sbjct: 301 --------------VLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIE 360

Query: 361 ESDVPKLPYLQGIISETLRLNPAAPMLVPHLTSDDCTISGYDIPRDTIVLVNAWAIHRDP 420
           E D+PKL YL  IISET RL PAAPMLVPH +SDDC + GYD+P+ TI+LVNAWAIHRDP
Sbjct: 361 EQDLPKLTYLHNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDP 420

Query: 421 NLWEEPTLFKPERHQKSSELGEHQVHKLVPFGVGRRACPGSGMAQRVVGLALAALIQCYE 480
             W+EPTLFKPERH       E +  KL+PFG+GRR+CPGSG+AQRVVGL L ALIQC+E
Sbjct: 421 EFWDEPTLFKPERHGGV----ELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFE 480

Query: 481 WQRIGEEKIDMTEGRGVTMPKAVPLEVMCKPRRIIHNL 513
           W+RIGE KIDM EG G+TMPKA PLE +CKPR I+H +
Sbjct: 481 WKRIGEAKIDMAEGSGLTMPKAQPLEALCKPRNILHKV 494

BLAST of CmUC01G016370 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 2.6e-166
Identity = 291/505 (57.62%), Postives = 377/505 (74.65%), Query Frame = 0

Query: 6   LLCLFLSLISLIFAFNLRRR-RNLPPSPLSLPIIGHLHYLKIPVHRTFQNLSAKYGPVIS 65
           ++ LF  +I+L   FN  R+ +NLPP P  LPIIG+LH LK P+H TF  LS KYG + S
Sbjct: 9   IISLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQIFS 68

Query: 66  LWFGSRLVVVVSSLDVMEECFTKNDIVLANRPRLLVGKYIGYNHTTMVASPYGEHWRNLR 125
           LWFGSRLVVVVSSL + +ECFTKNDIVLANRP  L GKYIGYN+TT+  SPYG+HWRNLR
Sbjct: 69  LWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNLR 128

Query: 126 RIGAIEIFSASRLNKLAGIRKDEVERLLRKLSRNSLNGFSKVHMQSAISELTFNISMRMA 185
           RI +IEI S+ RLN    IR+DE+ RL++KL++ S NGF++V ++   SE+TFN  MRM 
Sbjct: 129 RILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIMRMV 188

Query: 186 AGKRYFGE--EVTDEEEARQFGDLIKQVVALGGVSNPGDFIPMINWIP-NGFKRKVSGLA 245
           +GKRY+G   +V+D EEAR F  +IK+VV+LGG +N GDF+  + W   +G ++++  ++
Sbjct: 189 SGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLKKIS 248

Query: 246 KRTDAFLQGLIEEHRSKKEEGRNTMIDHLLSLQESEPENYGDQIIKGIILLKMPQIFFIN 305
           KRTDAFLQGLI+EHR  K    NTMIDHLL+ Q+S+PE Y DQIIKG+++          
Sbjct: 249 KRTDAFLQGLIDEHRFGKRNS-NTMIDHLLTQQQSQPEYYTDQIIKGLMV---------- 308

Query: 306 IVIIKRHVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDAQIGEDRLVEESDVPKL 365
                  V+LLAGTDTS+VTIEWA+++LLN+PE++KKA+ ELD  IG DR V+E D+ KL
Sbjct: 309 -------VMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKL 368

Query: 366 PYLQGIISETLRLNPAAPMLVPHLTSDDCTISGYDIPRDTIVLVNAWAIHRDPNLWEEPT 425
           PYLQ I+ ETLRL+ AAP+LVPHL+S+D ++ GY+IP++TI++VNAW IHRDPNLW +PT
Sbjct: 369 PYLQSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPT 428

Query: 426 LFKPERHQKSSELGEHQVHKLVPFGVGRRACPGSGMAQRVVGLALAALIQCYEWQRIGEE 485
            FKPER +K     E +V+KL+ FG+GRRACPG  ++QR  GL L  LIQC+EW+RIGEE
Sbjct: 429 CFKPERFEK-----EGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEE 488

Query: 486 KIDMTEGRGVTMPKAVPLEVMCKPR 507
           KIDM E +G+T  K   L  MCK R
Sbjct: 489 KIDMVEAKGITAGKKTSLNAMCKVR 490

BLAST of CmUC01G016370 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 567.0 bits (1460), Expect = 2.2e-160
Identity = 278/520 (53.46%), Postives = 381/520 (73.27%), Query Frame = 0

Query: 1   MENYSLLCLFLSLISLIFAFNL----RRRRNLPPSPLSLPIIGHLHYLKIPVHRTFQNLS 60
           ME  SLL   +  ++L F FN+    R+ +NLPP P SLPIIG+LH+LK P+HRTF+ LS
Sbjct: 1   MEILSLLSYSVFYLALFFIFNIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLS 60

Query: 61  AKYGPVISLWFGSRLVVVVSSLDVMEECFTKNDIVLANRPRLLVGKYIGYNHTTMVASPY 120
            KYG V SLWFGSRLVVVVSS    ++CFTKND+VLANRPR L GKYI YN+TT+ ++ Y
Sbjct: 61  EKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSY 120

Query: 121 GEHWRNLRRIGAIEIFSASRLNKLAGIRKDEVERLLRKLSRNSLNGFSKVHMQSAISELT 180
           GEHWRNLRRI A+++ S  R+N  +GIR+DE +RL+ +L+ +S   F+++ + S + ++T
Sbjct: 121 GEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMT 180

Query: 181 FNISMRMAAGKRYFGE--EVTDEEEARQFGDLIKQVVALGGVSNPGDFIPMINWIP-NGF 240
           FN  MRM +GKRY+GE  + +D +EA QF D++ +++ L G +N  DF+P++ ++     
Sbjct: 181 FNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENL 240

Query: 241 KRKVSGLAKRTDAFLQGLIEEHRSKKEEGRNTMIDHLLSLQESEPENYGDQIIKGIILLK 300
           ++++  ++ +TDAFL+GLIEEHR+KKE   NTMIDHLL+LQ+S+PE Y DQIIKG+ L  
Sbjct: 241 EKRLKDISGKTDAFLRGLIEEHRTKKERA-NTMIDHLLNLQDSQPEYYTDQIIKGLAL-- 300

Query: 301 MPQIFFINIVIIKRHVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDAQIGEDRLV 360
                           +LLAGTD+SAVT+EW++++LLN+PEVLKK ++ELD  +G+DRLV
Sbjct: 301 ---------------AMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLV 360

Query: 361 EESDVPKLPYLQGIISETLRLNPAAPMLVPHLTSDDCTISGYDIPRDTIVLVNAWAIHRD 420
           +ESD+PKL YL+ +I+ETLRL   AP+L+PH TSD+C I GY +P+DTIVL+NAWAIHRD
Sbjct: 361 DESDLPKLTYLKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRD 420

Query: 421 PNLWEEPTLFKPERHQKSSELGEHQVHKLVPFGVGRRACPGSGMAQRVVGLALAALIQCY 480
           P LW E T FKPER +K  EL      KL+ FG+GRRACPG G+A R + + LA LIQC+
Sbjct: 421 PELWTEATTFKPERFEKKGEL-----EKLIAFGMGRRACPGEGLAIRAISMTLALLIQCF 480

Query: 481 EWQRIGEEKIDMTEGRGVTMPKAVPLEVMCKPRRIIHNLF 514
           +W+ I  +KID+ E  G T+ K VPL+ MCK R +I+ +F
Sbjct: 481 DWKLINGDKIDLAERDGFTLTKLVPLKAMCKSRPVINKVF 497

BLAST of CmUC01G016370 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 1.9e-156
Identity = 273/517 (52.80%), Postives = 373/517 (72.15%), Query Frame = 0

Query: 4   YSLLCL-FLSLISLIFAFNLRRRRNLPPSPLSLPIIGHLHYLKIPVHRTFQNLSAKYGPV 63
           YSLL L F+  I ++     RR +NLPP P ++PIIG+LH+LK P+HRTF  LS  YG +
Sbjct: 5   YSLLSLSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDI 64

Query: 64  ISLWFGSRLVVVVSSLDVMEECFTKNDIVLANRPRLLVGKYIGYNHTTMVASPYGEHWRN 123
            SLWFGSRLVVVVSS  +  ECFTKNDI+LANRPR L GKYI YN+TT+ ++ YG+HWRN
Sbjct: 65  FSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRN 124

Query: 124 LRRIGAIEIFSASRLNKLAGIRKDEVERLLRKLSRNSLN---GFSKVHMQSAISELTFNI 183
           LRRI  I++ S +RLN   G+R+DE  RL++KL ++ ++   GF+KV ++  ++E+TFN 
Sbjct: 125 LRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNA 184

Query: 184 SMRMAAGKRYFGE--EVTDEEEARQFGDLIKQVVALGGVSNPGDFIPMINWIP-NGFKRK 243
            MRM +GKRY+G+  +V+D EEA+QF ++I ++++L G +N GDF+P++  +  +  +++
Sbjct: 185 MMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKR 244

Query: 244 VSGLAKRTDAFLQGLIEEH-RSKKEEGRNTMIDHLLSLQESEPENYGDQIIKGIILLKMP 303
              +AKR++AFL+GLIEEH R        TMIDHLL L ES+PE Y D +IKG+I     
Sbjct: 245 CKRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLI----- 304

Query: 304 QIFFINIVIIKRHVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDAQIGEDRLVEE 363
                         +LLAGTDTSAVTIEW ++ LLN+PEVLKKA+EELD QIG+++LV+E
Sbjct: 305 ------------QGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDE 364

Query: 364 SDVPKLPYLQGIISETLRLNPAAPMLVPHLTSDDCTISGYDIPRDTIVLVNAWAIHRDPN 423
            D+ KLPYLQ IISETLRL+P AP+L+PH +S+DCTI  +++P+DTI+L N W IHRDP 
Sbjct: 365 QDLSKLPYLQNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPK 424

Query: 424 LWEEPTLFKPERHQKSSELGEHQVHKLVPFGVGRRACPGSGMAQRVVGLALAALIQCYEW 483
            W +   FKPER +K     E +V+K++ FG+GRRACPG  +AQR VG  +  LIQC+EW
Sbjct: 425 HWNDALSFKPERFEK-----EEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEW 484

Query: 484 QRIGEEKIDMTEGRGVTMPKAVPLEVMCKPRRIIHNL 513
           +R  EEK+DM EG+G+TMP  +PL  MCK   I +++
Sbjct: 485 ERESEEKLDMMEGKGITMPMKIPLRAMCKALPIANDV 499

BLAST of CmUC01G016370 vs. ExPASy Swiss-Prot
Match: Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 1.6e-155
Identity = 268/516 (51.94%), Postives = 371/516 (71.90%), Query Frame = 0

Query: 5   SLLCLFLSLISLIF----AFNLRRRRNLPPSPLSLPIIGHLHYLKIPVHRTFQNLSAKYG 64
           S LC  L  +S+ F     F  R+ +NLPP P SLPIIG+LH+LK P++RTF+ L+ KYG
Sbjct: 5   SYLCYSLFYLSIFFIIRLLFQSRKFKNLPPGPTSLPIIGNLHHLKRPLNRTFKALTEKYG 64

Query: 65  PVISLWFGSRLVVVVSSLDVMEECFTKNDIVLANRPRLLVGKYIGYNHTTMVASPYGEHW 124
            VISLWFGSRLVVVVSSL   +ECFTKND+VLANRPR L GKYI YN+TT+ ++ YGEHW
Sbjct: 65  NVISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHW 124

Query: 125 RNLRRIGAIEIFSASRLNKLAGIRKDEVERLLRKLSRNSLNGFSKVHMQSAISELTFNIS 184
           RNLRRI ++++ S  R+N  A IR+DE +RL++KL+ +S   F++V +     ++TFN  
Sbjct: 125 RNLRRITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMTFNNI 184

Query: 185 MRMAAGKRYFGE--EVTDEEEARQFGDLIKQVVALGGVSNPGDFIPMINWIP-NGFKRKV 244
           MRM +GKRY+G+  ++++ +EA QF D++ +++ L G +N  DF+P++ ++     +++V
Sbjct: 185 MRMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKRV 244

Query: 245 SGLAKRTDAFLQGLIEEHRSKKEEGRNTMIDHLLSLQESEPENYGDQIIKGIILLKMPQI 304
             + ++ D FL GL++E RSKKE   NTMIDHLL++QES+PE Y D IIKG+ L      
Sbjct: 245 KRIGEKNDVFLSGLLQEQRSKKER-TNTMIDHLLNMQESQPEYYTDTIIKGLCL------ 304

Query: 305 FFINIVIIKRHVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDAQIGEDRLVEESD 364
                       +LLAGTD+SAVT+EW ++++LN PEVLKK R+E+D  +G+DRLV+ESD
Sbjct: 305 -----------AMLLAGTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESD 364

Query: 365 VPKLPYLQGIISETLRLNPAAPMLVPHLTSDDCTISGYDIPRDTIVLVNAWAIHRDPNLW 424
           +PKL YL+ +I ETLRL   AP+L+PH T+D+C + GY +PRDTIVL+NAWAIHRDP  W
Sbjct: 365 LPKLTYLRNVIYETLRLYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETW 424

Query: 425 EEPTLFKPERHQKSSELGEHQVHKLVPFGVGRRACPGSGMAQRVVGLALAALIQCYEWQR 484
            E T FKPER  K  EL      K++ FG+GRRACPG G+A R + + LA L+QC++W+R
Sbjct: 425 SEATTFKPERFDKKGEL-----EKMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKR 484

Query: 485 IGEEKIDMTEGRGVTMPKAVPLEVMCKPRRIIHNLF 514
           I +EKIDM+E  G TM K +PL+ MCK R +++ +F
Sbjct: 485 INDEKIDMSERDGFTMTKLLPLKAMCKTRPVVNKVF 497

BLAST of CmUC01G016370 vs. ExPASy TrEMBL
Match: A0A1S3B1G8 (cytochrome P450 81E8 OS=Cucumis melo OX=3656 GN=LOC103484810 PE=3 SV=1)

HSP 1 Score: 857.4 bits (2214), Expect = 3.0e-245
Identity = 432/515 (83.88%), Postives = 459/515 (89.13%), Query Frame = 0

Query: 1   MENYSLLCLFLSLISLIFAFNLR-RRRNLPPSPLSLPIIGHLHYLKIPVHRTFQNLSAKY 60
           ME YSLL LFLSLISL+FAFNLR  RRNLPPSP SLPIIGHLHYLKIP+HRT Q LSAKY
Sbjct: 1   MEIYSLLYLFLSLISLLFAFNLRSSRRNLPPSPPSLPIIGHLHYLKIPLHRTLQKLSAKY 60

Query: 61  GPVISLWFGSRLVVVVSSLDVMEECFTKNDIVLANRPRLLVGKYIGYNHTTMVASPYGEH 120
           GPVISLWFGSRLVVVVSSL  +EECFTKNDIVLANRPRLLVGK++GYNHTTMVASPYG+H
Sbjct: 61  GPVISLWFGSRLVVVVSSLPAVEECFTKNDIVLANRPRLLVGKHVGYNHTTMVASPYGDH 120

Query: 121 WRNLRRIGAIEIFSASRLNKLAGIRKDEVERLLRKLSRNSLNGFSKVHMQSAISELTFNI 180
           WRNLRRIGAIEIFSASRLNK AG RKDEVERLL+KLSRNS+NGFSKV MQSAI+ELTFNI
Sbjct: 121 WRNLRRIGAIEIFSASRLNKFAGTRKDEVERLLKKLSRNSMNGFSKVEMQSAIAELTFNI 180

Query: 181 SMRMAAGKRYFGEEVTDEEEARQFGDLIKQVVALGGVSNPGDFIPMINWIPNGFKRKVSG 240
           SMRMAAGKRYFGEEVTD EEARQ  +LIKQ+V+LGGVSNPGDFIP++NWIPNGF+RKVS 
Sbjct: 181 SMRMAAGKRYFGEEVTDVEEARQSRELIKQIVSLGGVSNPGDFIPLMNWIPNGFRRKVSR 240

Query: 241 LAKRTDAFLQGLIEEHRSKKEEGRNTMIDHLLSLQESEPENYGDQIIKGIILLKMPQIFF 300
           LAKR D FLQGLI+EHRS K E RNTMIDHLLSLQESEPE YGDQIIKGIIL        
Sbjct: 241 LAKRMDGFLQGLIDEHRSNKCEERNTMIDHLLSLQESEPEYYGDQIIKGIIL-------- 300

Query: 301 INIVIIKRHVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDAQIGEDRLVEESDVP 360
                    VLLLAGTDTSAVTIEWALAHLLNN EVLKKAREELD QIGE RLVEESD+ 
Sbjct: 301 ---------VLLLAGTDTSAVTIEWALAHLLNNLEVLKKAREELDTQIGEKRLVEESDIL 360

Query: 361 KLPYLQGIISETLRLNPAAPMLVPHLTSDDCTISGYDIPRDTIVLVNAWAIHRDPNLWEE 420
           KLPYLQGIISETLRLNPAAPMLVPHLTS+DCTISGY IPRDTIVLVNAWAIHR+PN WEE
Sbjct: 361 KLPYLQGIISETLRLNPAAPMLVPHLTSNDCTISGYKIPRDTIVLVNAWAIHREPNQWEE 420

Query: 421 PTLFKPERHQKSSELGEHQVHKLVPFGVGRRACPGSGMAQRVVGLALAALIQCYEWQRIG 480
           PTLFKPERHQKS     HQ+ KL+PFG+GRRACPGSGMAQRVVGL LAALIQCYEW+RIG
Sbjct: 421 PTLFKPERHQKSESSSHHQIPKLIPFGIGRRACPGSGMAQRVVGLTLAALIQCYEWERIG 480

Query: 481 EEKIDMTEGRGVTMPKAVPLEVMCKPRRIIHNLFN 515
           EEK+DM EGRGVTMPK +PLE MCKPR IIH++FN
Sbjct: 481 EEKVDMAEGRGVTMPKLLPLEAMCKPRPIIHHIFN 498

BLAST of CmUC01G016370 vs. ExPASy TrEMBL
Match: A0A0A0KJM0 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa_6G492230 PE=3 SV=1)

HSP 1 Score: 850.1 bits (2195), Expect = 4.7e-243
Identity = 430/515 (83.50%), Postives = 458/515 (88.93%), Query Frame = 0

Query: 1   MENYSLLCLFLSLISLIFAFNLR-RRRNLPPSPLSLPIIGHLHYLKIPVHRTFQNLSAKY 60
           ME  SLL LFLSLISL+FAFNLR RRRNLPPSP SLPIIGHLHY+KIP HRT   LSAKY
Sbjct: 1   MEISSLLYLFLSLISLLFAFNLRSRRRNLPPSPPSLPIIGHLHYIKIPRHRTLHKLSAKY 60

Query: 61  GPVISLWFGSRLVVVVSSLDVMEECFTKNDIVLANRPRLLVGKYIGYNHTTMVASPYGEH 120
           GPVISLWFGSRLVVVVSSL  +EECFTKNDIVLANRPRLLVGK++GYNHTTMVASPYG+H
Sbjct: 61  GPVISLWFGSRLVVVVSSLPAVEECFTKNDIVLANRPRLLVGKHVGYNHTTMVASPYGDH 120

Query: 121 WRNLRRIGAIEIFSASRLNKLAGIRKDEVERLLRKLSRNSLNGFSKVHMQSAISELTFNI 180
           WRNLRRIGAIEIFSASRLNK AG RKDEVERLL+KLSRNS+ GFSKV MQS+ISELTFNI
Sbjct: 121 WRNLRRIGAIEIFSASRLNKFAGTRKDEVERLLKKLSRNSMYGFSKVEMQSSISELTFNI 180

Query: 181 SMRMAAGKRYFGEEVTDEEEARQFGDLIKQVVALGGVSNPGDFIPMINWIPNGFKRKVSG 240
           SMRMAAGKRYFGEEV D EEARQ  +LIKQ+V++GGVSNPGDFIPM+NWIPNGFKRKV  
Sbjct: 181 SMRMAAGKRYFGEEVRDVEEARQSRELIKQIVSMGGVSNPGDFIPMMNWIPNGFKRKVWR 240

Query: 241 LAKRTDAFLQGLIEEHRSKKEEGRNTMIDHLLSLQESEPENYGDQIIKGIILLKMPQIFF 300
           +AKR D FLQGLI+EHRS KEE RNTMIDHLLSLQE+EPE YGDQIIKGIIL        
Sbjct: 241 VAKRMDGFLQGLIDEHRSNKEEERNTMIDHLLSLQETEPEYYGDQIIKGIIL-------- 300

Query: 301 INIVIIKRHVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDAQIGEDRLVEESDVP 360
                    VLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELD QIGE RLVEESDV 
Sbjct: 301 ---------VLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDTQIGEKRLVEESDVS 360

Query: 361 KLPYLQGIISETLRLNPAAPMLVPHLTSDDCTISGYDIPRDTIVLVNAWAIHRDPNLWEE 420
           KLPYLQGIISETLRLNPAAPMLVPHLTS+DCTISGY IPRDTIVLVNAWAIHRDP+ WEE
Sbjct: 361 KLPYLQGIISETLRLNPAAPMLVPHLTSNDCTISGYKIPRDTIVLVNAWAIHRDPHQWEE 420

Query: 421 PTLFKPERHQKSSELGEHQVHKLVPFGVGRRACPGSGMAQRVVGLALAALIQCYEWQRIG 480
           PTLFKPERHQKS  + +H + KL+PFGVGRRACPGSGMAQRVVGL LAALIQCYEW+RIG
Sbjct: 421 PTLFKPERHQKSESI-DHHISKLIPFGVGRRACPGSGMAQRVVGLTLAALIQCYEWERIG 480

Query: 481 EEKIDMTEGRGVTMPKAVPLEVMCKPRRIIHNLFN 515
           +EK+DM+EGRGVTMPK VPLE MCKPR IIHN+FN
Sbjct: 481 DEKVDMSEGRGVTMPKMVPLEAMCKPRPIIHNIFN 497

BLAST of CmUC01G016370 vs. ExPASy TrEMBL
Match: A0A6J1IVD6 (cytochrome P450 81E8-like OS=Cucurbita maxima OX=3661 GN=LOC111478736 PE=3 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 2.2e-240
Identity = 418/514 (81.32%), Postives = 460/514 (89.49%), Query Frame = 0

Query: 1   MENYSLLCLFLSLISLIFAFNLRRRRNLPPSPLSLPIIGHLHYLKIPVHRTFQNLSAKYG 60
           ME+ SLL L LSLI L+FAF+ RRRRNLPP P SLPIIGHLHYLK+PVHRT Q LSAKYG
Sbjct: 1   MEDRSLLYLLLSLIFLLFAFSPRRRRNLPPGPPSLPIIGHLHYLKVPVHRTLQKLSAKYG 60

Query: 61  PVISLWFGSRLVVVVSSLDVMEECFTKNDIVLANRPRLLVGKYIGYNHTTMVASPYGEHW 120
           PVISLWFGSRLVVVVSS  V+EECFTKND+VLANRPRLL+GK++GYN+TTMVASPYG+HW
Sbjct: 61  PVISLWFGSRLVVVVSSSAVVEECFTKNDVVLANRPRLLLGKHVGYNYTTMVASPYGDHW 120

Query: 121 RNLRRIGAIEIFSASRLNKLAGIRKDEVERLLRKLSRNSLNGFSKVHMQSAISELTFNIS 180
           RNLRRIGAIEIFSASRLNK AGIR+DEVERLLRKLS+NSL+GFSKV MQS ISELTFNIS
Sbjct: 121 RNLRRIGAIEIFSASRLNKFAGIRRDEVERLLRKLSKNSLHGFSKVQMQSTISELTFNIS 180

Query: 181 MRMAAGKRYFGEEVTDEEEARQFGDLIKQVVALGGVSNPGDFIPMINWIPNGFKRKVSGL 240
           M+MAAGKRYFG++V DE+EARQF +LIKQ+V+LGGVSNPGDFIPM+NW PN F+RKVS L
Sbjct: 181 MKMAAGKRYFGDDVADEDEARQFRELIKQIVSLGGVSNPGDFIPMLNWFPNVFERKVSRL 240

Query: 241 AKRTDAFLQGLIEEHRSKKEEGRNTMIDHLLSLQESEPENYGDQIIKGIILLKMPQIFFI 300
           AKRTDAFLQGLI+EHRS KEE RNTMIDHLLSLQESEPE YGDQIIKGIIL         
Sbjct: 241 AKRTDAFLQGLIDEHRSNKEEERNTMIDHLLSLQESEPELYGDQIIKGIIL--------- 300

Query: 301 NIVIIKRHVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDAQIGEDRLVEESDVPK 360
                   VLLLAGTDTSAVT+EWAL+HLLNNPE+LKKAREELDAQIG++RLVEESDVPK
Sbjct: 301 --------VLLLAGTDTSAVTMEWALSHLLNNPEILKKAREELDAQIGQERLVEESDVPK 360

Query: 361 LPYLQGIISETLRLNPAAPMLVPHLTSDDCTISGYDIPRDTIVLVNAWAIHRDPNLWEEP 420
           LPYLQGIISETLRLNPAAP+L+PHLTSDDCTIS Y +PRDTIVLVNAWAIHRDP+ WE+P
Sbjct: 361 LPYLQGIISETLRLNPAAPLLLPHLTSDDCTISNYKVPRDTIVLVNAWAIHRDPSQWEDP 420

Query: 421 TLFKPERHQKSSELGEHQVHKLVPFGVGRRACPGSGMAQRVVGLALAALIQCYEWQRIGE 480
           T F PERH+KS   G+H  HK +PFG+GRR+CPGSGMAQRVVGLALAALIQCYEW+RIGE
Sbjct: 421 TRFNPERHRKSE--GDH-AHKFLPFGIGRRSCPGSGMAQRVVGLALAALIQCYEWERIGE 480

Query: 481 EKIDMTEGRGVTMPKAVPLEVMCKPRRIIHNLFN 515
           EK+DM EGRG+TMPK VPLE MCKPR IIHNLFN
Sbjct: 481 EKVDMAEGRGLTMPKVVPLEAMCKPRPIIHNLFN 494

BLAST of CmUC01G016370 vs. ExPASy TrEMBL
Match: A0A6J1GGA4 (cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111453889 PE=3 SV=1)

HSP 1 Score: 840.5 bits (2170), Expect = 3.8e-240
Identity = 418/514 (81.32%), Postives = 459/514 (89.30%), Query Frame = 0

Query: 1   MENYSLLCLFLSLISLIFAFNLRRRRNLPPSPLSLPIIGHLHYLKIPVHRTFQNLSAKYG 60
           ME+ SLL L LSLI L+FA + RRRRNLPP P SLPIIGHLHYLK+PVHRT Q LSAKYG
Sbjct: 1   MEDRSLLYLLLSLIFLLFALSPRRRRNLPPGPPSLPIIGHLHYLKVPVHRTLQKLSAKYG 60

Query: 61  PVISLWFGSRLVVVVSSLDVMEECFTKNDIVLANRPRLLVGKYIGYNHTTMVASPYGEHW 120
           PVISLWFGSRLVVVVSS  V+EECFTKND+VLANRPRLLVGK++GYN+TTMVASPYG+HW
Sbjct: 61  PVISLWFGSRLVVVVSSSAVVEECFTKNDVVLANRPRLLVGKHVGYNYTTMVASPYGDHW 120

Query: 121 RNLRRIGAIEIFSASRLNKLAGIRKDEVERLLRKLSRNSLNGFSKVHMQSAISELTFNIS 180
           RNLRRIGAIEIFSASRLNK AGIR+DEVERLLRKLS+NSL+GFSKV MQS ISELTFNIS
Sbjct: 121 RNLRRIGAIEIFSASRLNKFAGIRRDEVERLLRKLSKNSLHGFSKVQMQSTISELTFNIS 180

Query: 181 MRMAAGKRYFGEEVTDEEEARQFGDLIKQVVALGGVSNPGDFIPMINWIPNGFKRKVSGL 240
           MRMAAGKRYFG++V DE+EARQF +LIKQ+V+LGGVSNPGDFIPM+NWIPN F+RKVS L
Sbjct: 181 MRMAAGKRYFGDDVADEDEARQFRELIKQIVSLGGVSNPGDFIPMLNWIPNVFERKVSRL 240

Query: 241 AKRTDAFLQGLIEEHRSKKEEGRNTMIDHLLSLQESEPENYGDQIIKGIILLKMPQIFFI 300
           AKRTDAFLQGLI+EHRS KEE RNTMIDHLLSLQ+SEPE YGDQIIKGIIL         
Sbjct: 241 AKRTDAFLQGLIDEHRSNKEEERNTMIDHLLSLQQSEPELYGDQIIKGIIL--------- 300

Query: 301 NIVIIKRHVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDAQIGEDRLVEESDVPK 360
                   VLLLAGTDTSAVT+EWAL+HLLNNPE+LKKAREELDAQIG++RLVEESDVPK
Sbjct: 301 --------VLLLAGTDTSAVTMEWALSHLLNNPEILKKAREELDAQIGQERLVEESDVPK 360

Query: 361 LPYLQGIISETLRLNPAAPMLVPHLTSDDCTISGYDIPRDTIVLVNAWAIHRDPNLWEEP 420
           LPYLQGIISETLRL PAAP+LVPHLTSDDCTIS Y +PRDTIVLVNAWAIHRDP+ WE+P
Sbjct: 361 LPYLQGIISETLRLTPAAPLLVPHLTSDDCTISNYKVPRDTIVLVNAWAIHRDPSQWEDP 420

Query: 421 TLFKPERHQKSSELGEHQVHKLVPFGVGRRACPGSGMAQRVVGLALAALIQCYEWQRIGE 480
           T F PERH+KS   G+H  HK +PFG+GRR+CPGSGMAQRVVGLALAA+IQCYEW+RIGE
Sbjct: 421 TRFNPERHRKSE--GDH-AHKFLPFGIGRRSCPGSGMAQRVVGLALAAMIQCYEWERIGE 480

Query: 481 EKIDMTEGRGVTMPKAVPLEVMCKPRRIIHNLFN 515
           EK+DM EGRG+TMPK VPLE MCKPR IIHNLFN
Sbjct: 481 EKVDMAEGRGITMPKVVPLEAMCKPRPIIHNLFN 494

BLAST of CmUC01G016370 vs. ExPASy TrEMBL
Match: A0A0A0KLF2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G492220 PE=3 SV=1)

HSP 1 Score: 816.2 bits (2107), Expect = 7.6e-233
Identity = 409/515 (79.42%), Postives = 446/515 (86.60%), Query Frame = 0

Query: 1   MENYSLLCLFLSLISLIFAFNLRRR-RNLPPSPLSLPIIGHLHYLKIPVHRTFQNLSAKY 60
           ME  SLL LFLS+ISL+FAFNLR R  NLPPSP SLPIIGHLHY+KIP+HRT   LSAKY
Sbjct: 1   MECCSLLYLFLSIISLLFAFNLRTRPTNLPPSPPSLPIIGHLHYIKIPLHRTLHKLSAKY 60

Query: 61  GPVISLWFGSRLVVVVSSLDVMEECFTKNDIVLANRPRLLVGKYIGYNHTTMVASPYGEH 120
           GP+ISL FGSRLVVVVSSL  +EECFTKNDIVLANRPRLL GK+IGYNHTTMV SPYG+H
Sbjct: 61  GPLISLRFGSRLVVVVSSLPAVEECFTKNDIVLANRPRLLFGKHIGYNHTTMVGSPYGDH 120

Query: 121 WRNLRRIGAIEIFSASRLNKLAGIRKDEVERLLRKLSRNSLNGFSKVHMQSAISELTFNI 180
           WRNLRRIGAIEIFSASRLNK AG RKDEVERLL+KLSRNS++G+SKV MQS+ISELTFNI
Sbjct: 121 WRNLRRIGAIEIFSASRLNKFAGTRKDEVERLLKKLSRNSMHGYSKVEMQSSISELTFNI 180

Query: 181 SMRMAAGKRYFGEEVTDEEEARQFGDLIKQVVALGGVSNPGDFIPMINWIPNGFKRKVSG 240
           SMRMAAGKRYFG+EV D EEARQ  +LIKQ+V++GGVSNPGDFIPM+NW+PNG+KRKVS 
Sbjct: 181 SMRMAAGKRYFGDEVRDVEEARQSRELIKQIVSMGGVSNPGDFIPMMNWVPNGYKRKVSR 240

Query: 241 LAKRTDAFLQGLIEEHRSKKEEGRNTMIDHLLSLQESEPENYGDQIIKGIILLKMPQIFF 300
           L KR D FLQGLI+EHRS KEE RNTMIDHLLSLQE+EPE YGD+IIKGI+L        
Sbjct: 241 LGKRMDRFLQGLIDEHRSNKEEERNTMIDHLLSLQETEPEYYGDEIIKGIVL-------- 300

Query: 301 INIVIIKRHVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDAQIGEDRLVEESDVP 360
                    VLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELD QIGE +L EE DV 
Sbjct: 301 ---------VLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDNQIGEKQLAEELDVS 360

Query: 361 KLPYLQGIISETLRLNPAAPMLVPHLTSDDCTISGYDIPRDTIVLVNAWAIHRDPNLWEE 420
           KLPYLQGII ETLRLNPAAPMLVPHLTS++CTIS Y+IPRDTIVLVNAWAIHRDP+ WEE
Sbjct: 361 KLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTIVLVNAWAIHRDPSQWEE 420

Query: 421 PTLFKPERHQKSSELGEHQVHKLVPFGVGRRACPGSGMAQRVVGLALAALIQCYEWQRIG 480
           PTLFKPERHQ       HQ+ K++PFGVGRRACPGS MAQRVVGL LA LIQCYEW+RIG
Sbjct: 421 PTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMAQRVVGLTLATLIQCYEWERIG 480

Query: 481 EEKIDMTEGRGVTMPKAVPLEVMCKPRRIIHNLFN 515
           EEK+DMTEGRGVTMPK VPLE MCK R I+HN+FN
Sbjct: 481 EEKVDMTEGRGVTMPKVVPLEAMCKARPIVHNIFN 498

BLAST of CmUC01G016370 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 560.1 bits (1442), Expect = 1.9e-159
Identity = 285/514 (55.45%), Postives = 377/514 (73.35%), Query Frame = 0

Query: 4   YSLLCLFLSLISLIFAFNLRRRRNLPPSPL-SLPIIGHLHYLKIPVHRTFQNLSAKY--G 63
           +S+L + LSLI LI    L+R+ NLPPSP  SLP+IGHL  LK P+HRTF +LS      
Sbjct: 8   FSILFVVLSLIYLI--GKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNA 67

Query: 64  PVISLWFGSRLVVVVSSLDVMEECFTKNDIVLANRPRLLVGKYIGYNHTTMVASPYGEHW 123
           P+ SL  G+RLV V SS  + EECFTKND+VLANRP  ++ K++ Y++TTM+A+ YG+HW
Sbjct: 68  PIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHW 127

Query: 124 RNLRRIGAIEIFSASRLNKLAGIRKDEVERLLRKLSRNSLNGFSKVHMQSAISELTFNIS 183
           RNLRRIG++EIFS  RLN    IRKDE+ RL+ +LSRN    F KV M+S +S+LTFN  
Sbjct: 128 RNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNI 187

Query: 184 MRMAAGKRYFGEEVTDEEEARQFGDLIKQVVALGGVSNPGDFIPMINWIPNGFKRKVSGL 243
           +RM AGKRY+G+ V D+ EA++   LI  VVA  G  N  D++P++  + + ++ +V  L
Sbjct: 188 LRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV-SDYETRVKKL 247

Query: 244 AKRTDAFLQGLIEEHRSKKEEGRNTMIDHLLSLQESEPENYGDQIIKGIILLKMPQIFFI 303
           A R D FLQGL++E R  KE+G NTMIDHLL+LQES+P+ + D+IIKG +L         
Sbjct: 248 AGRLDEFLQGLVDEKREAKEKG-NTMIDHLLTLQESQPDYFTDRIIKGNML--------- 307

Query: 304 NIVIIKRHVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDAQIGEDRLVEESDVPK 363
                    L+LAGTDTSAVT+EWAL+++LN+P+VL KAR+E+D +IG DRL++ESD+  
Sbjct: 308 --------ALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISN 367

Query: 364 LPYLQGIISETLRLNPAAPMLVPHLTSDDCTISGYDIPRDTIVLVNAWAIHRDPNLWEEP 423
           LPYLQ I+SETLRL PAAPML+PH+ S+DC ++GYD+PR TI+L N WAIHRDP LW++P
Sbjct: 368 LPYLQNIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDP 427

Query: 424 TLFKPERHQKSSELGEHQVHKLVPFGVGRRACPGSGMAQRVVGLALAALIQCYEWQRIGE 483
             FKPER +K     E +  KL+PFG+GRRACPGSG+A R++ L L +LIQC EW++IGE
Sbjct: 428 MSFKPERFEK-----EGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGE 487

Query: 484 EKIDMTEGRGVTMPKAVPLEVMCKPRRIIHNLFN 515
           E +DM+EG+GVTMPKA PLE MC+ R  +  +FN
Sbjct: 488 E-VDMSEGKGVTMPKAKPLEAMCRARPSVVKIFN 494

BLAST of CmUC01G016370 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 554.3 bits (1427), Expect = 1.0e-157
Identity = 282/514 (54.86%), Postives = 370/514 (71.98%), Query Frame = 0

Query: 1   MENYSLLCLFLSLI-SLIFAF-NLRRRRNLPPSPL-SLPIIGHLHYLKIPVHRTFQNLSA 60
           ME   L+  F  ++ SLIF    ++R+ NLPPSP  +LP+IGHL  LK P+HR F ++S 
Sbjct: 1   MEALMLIFTFCFIVLSLIFLIGRIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQ 60

Query: 61  KYG--PVISLWFGSRLVVVVSSLDVMEECFTKNDIVLANRPRLLVGKYIGYNHTTMVASP 120
             G  P+ISL  G+RL+ VVSS  + EECFTKND++LANR   +  K+I Y ++T+V++ 
Sbjct: 61  SLGDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSAS 120

Query: 121 YGEHWRNLRRIGAIEIFSASRLNKLAGIRKDEVERLLRKLSRNSLNGFSKVHMQSAISEL 180
           Y EHWRNLRRIGA+EIFSA RLN  + IR+DE+ RL+ +L RNS  GF+KV M+S  S+L
Sbjct: 121 YSEHWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDL 180

Query: 181 TFNISMRMAAGKRYFGEEVTDEEEARQFGDLIKQVVALGGVSNPGDFIPMINWIPNGFKR 240
           TFN  +RM AGK Y+G+   D+ EA++   LI + ++  G  N  D+IP++ WI    + 
Sbjct: 181 TFNNIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS-ET 240

Query: 241 KVSGLAKRTDAFLQGLIEEHRSKKEEGRNTMIDHLLSLQESEPENYGDQIIKGIILLKMP 300
           ++  LA R D FLQGL++E R  KE+  NTM+DHLL LQE++PE Y D+IIKG +L    
Sbjct: 241 RIKKLAGRLDEFLQGLVDEKREGKEKKENTMVDHLLCLQETQPEYYMDRIIKGTML---- 300

Query: 301 QIFFINIVIIKRHVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDAQIGEDRLVEE 360
                         L+  GTDT+AVT+EWAL+ LLNNPEVL KAR+E+D  IG DRL+EE
Sbjct: 301 -------------SLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEE 360

Query: 361 SDVPKLPYLQGIISETLRLNPAAPMLVPHLTSDDCTISGYDIPRDTIVLVNAWAIHRDPN 420
           SD+P LPYLQ I+SETLRL PAAPML+PH+ S DC + GYD+PR T++L NAWAIHRDP 
Sbjct: 361 SDIPNLPYLQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPL 420

Query: 421 LWEEPTLFKPERHQKSSELGEHQVHKLVPFGVGRRACPGSGMAQRVVGLALAALIQCYEW 480
           LW++PT FKPER +K     E +  KL+PFG+GRRACPGSG+AQR+V L+L +LIQC+EW
Sbjct: 421 LWDDPTSFKPERFEK-----EGEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEW 480

Query: 481 QRIGEEKIDMTEGRGVTMPKAVPLEVMCKPRRII 510
           +RIGEE++DMTEG G+TMPKA PLE MC+ R  +
Sbjct: 481 ERIGEEEVDMTEGPGLTMPKARPLEAMCRARDFV 491

BLAST of CmUC01G016370 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 545.4 bits (1404), Expect = 4.8e-155
Identity = 278/520 (53.46%), Postives = 371/520 (71.35%), Query Frame = 0

Query: 1   MENYSLLCLFLSL-ISLIFAF-NLRRRRNLPPSP-LSLPIIGHLHYLKIPVHRTFQNLSA 60
           ME  +L+  FL + +SL F    ++RR NLPPSP  +LP+IGHL  LK P+HR F ++S 
Sbjct: 1   MEGQTLIFTFLFISLSLTFIIGRIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSE 60

Query: 61  KYG--PVISLWFGSRLVVVVSSLDVMEECFTKNDIVLANRPRLLVGKYIGYNHTTMVASP 120
             G  P+ISL  G+RLV VVSS  + EECFTKND+VLANR   L  K+I Y  TT+V + 
Sbjct: 61  SLGDAPIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTAS 120

Query: 121 YGEHWRNLRRIGAIEIFSASRLNKLAGIRKDEVERLLRKLSRNSLNGFSKVHMQSAISEL 180
           YG+HWRNLRRIGA+EIFSA RLN  + IR+DE+ RL+  LSRNS   F+KV M+S  S L
Sbjct: 121 YGDHWRNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNL 180

Query: 181 TFNISMRMAAGKRYFGEEVTDEEEARQFGDLIKQVVALGGVSNPGDFIPMINWIPNGFKR 240
           TFN  +RM AGK Y+G+   D+ EA++  +LI + +   G  N  D++P++ WI  G ++
Sbjct: 181 TFNNIIRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWI-TGSEK 240

Query: 241 KVSGLAKRTDAFLQGLIEEHRSKKEEGRNTMIDHLLSLQESEPENYGDQIIKGIILLKMP 300
           ++  +A R D FLQGL++E R  KE+ +NTM+DHLL LQE++PE Y D IIKGI+L    
Sbjct: 241 RIKKIASRLDEFLQGLVDERREGKEKRQNTMVDHLLCLQETQPEYYTDNIIKGIML---- 300

Query: 301 QIFFINIVIIKRHVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDAQIGEDRLVEE 360
                         L+LAGTDTSAVT+EW L+ LLN+P++L KAR+E+D ++G +RLVEE
Sbjct: 301 -------------SLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEE 360

Query: 361 SDVPKLPYLQGIISETLRLNPAAPMLVPHLTSDDCTISGYDIPRDTIVLVNAWAIHRDPN 420
           SD+  LPYLQ I+SE+LRL PA+P+LVPH+ S+DC + GY +PR T++L NAWAIHRDP 
Sbjct: 361 SDLSHLPYLQNIVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPK 420

Query: 421 LWEEPTLFKPERHQKSSELGEHQVHKLVPFGVGRRACPGSGMAQRVVGLALAALIQCYEW 480
           +W++PT FKPER +K     E +  KL+ FG+GRRACPGSG+AQR+  L + +LIQC+EW
Sbjct: 421 IWDDPTSFKPERFEK-----EGEAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEW 480

Query: 481 QRIGEEKIDMTE-GRGVTMPKAVPLEVMCKPRRIIHNLFN 515
           +RIGEE++DMTE G GV MPKA+PL  MCK R ++  + N
Sbjct: 481 ERIGEEEVDMTEGGGGVIMPKAIPLVAMCKARPVVGKILN 497

BLAST of CmUC01G016370 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 545.0 bits (1403), Expect = 6.3e-155
Identity = 269/504 (53.37%), Postives = 361/504 (71.63%), Query Frame = 0

Query: 9   LFLSLISLIFAFNLRRRRNLPPSP-LSLPIIGHLHYLKIPVHRTFQNLSAKYG--PVISL 68
           LFL++   +      R+ NLPPSP   LP+IGHLH LK PVHRTF ++S   G  P+  L
Sbjct: 12  LFLTISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSLGNAPIFHL 71

Query: 69  WFGSRLVVVVSSLDVMEECFTKNDIVLANRPRLLVGKYIGYNHTTMVASPYGEHWRNLRR 128
             G+RLV V+SS  + EECFTKND+VLANRP +++ K++GYN T M+A+ YG+HWRNLRR
Sbjct: 72  RLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYGDHWRNLRR 131

Query: 129 IGAIEIFSASRLNKLAGIRKDEVERLLRKLSRNSLNGFSKVHMQSAISELTFNISMRMAA 188
           I A+EIFS+ R++  + IRKDE+ RL+  LSR+SL+GF +V ++S ++ L FN  + M A
Sbjct: 132 IAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLAFNNIIMMVA 191

Query: 189 GKRYFGEEVTDEEEARQFGDLIKQVVALGGVSNPGDFIPMINWIPNGFKRKVSGLAKRTD 248
           GKRY+G    D +EA+   +LI +++A  G  N  D++P INW+ N F+ +   L  R D
Sbjct: 192 GKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWVTN-FENQTKILGNRLD 251

Query: 249 AFLQGLIEEHRSKKEEGRNTMIDHLLSLQESEPENYGDQIIKGIILLKMPQIFFINIVII 308
             LQ L++E R++KE+G+ T+IDHLLS QE+EPE Y D IIKGIIL              
Sbjct: 252 RVLQKLVDEKRAEKEKGQ-TLIDHLLSFQETEPEYYTDVIIKGIIL-------------- 311

Query: 309 KRHVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDAQIGEDRLVEESDVPKLPYLQ 368
               L+LAGTDTS+VT+EWA+++LLN+PE+L+KAR E+D +IG DRLVEESD+  L YLQ
Sbjct: 312 ---ALVLAGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQ 371

Query: 369 GIISETLRLNPAAPMLVPHLTSDDCTISGYDIPRDTIVLVNAWAIHRDPNLWEEPTLFKP 428
            I+SETLRL PA P+L+PH +SD+C ++GYD+PR T++L N WA+HRDP LWEEP  FKP
Sbjct: 372 NIVSETLRLYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKP 431

Query: 429 ERHQKSSELGEHQVHKLVPFGVGRRACPGSGMAQRVVGLALAALIQCYEWQRIGEEKIDM 488
           ER +K     E +  KL+PFG+GRRACPG+ + +R+V LAL  LIQ +EW+R+G E +DM
Sbjct: 432 ERFEK-----EGEARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDM 491

Query: 489 TEGRGVTMPKAVPLEVMCKPRRII 510
           TEG G+TMPKA PL  MCK R I+
Sbjct: 492 TEGEGITMPKATPLRAMCKARAIV 491

BLAST of CmUC01G016370 vs. TAIR 10
Match: AT3G28740.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 544.7 bits (1402), Expect = 8.2e-155
Identity = 288/518 (55.60%), Postives = 363/518 (70.08%), Query Frame = 0

Query: 4   YSLLCLFLSLI--SLIFAFNLR-RRRNLPPSP-LSLPIIGHLHYLKIPVHRTFQNLSAKY 63
           Y +L LF   I  SL   F  R R+ NLPPSP    PIIGHLH LK+P+HR F +LS   
Sbjct: 12  YLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSLSESL 71

Query: 64  --GPVISLWFGSRLVVVVSSLDVMEECFTKNDIVLANRPRLLVGKYIGYNHTTMVASPYG 123
               + SL  GSRLV VVSS  V EECFTKND+VLANRP  LVGK+IGYN TTMV + YG
Sbjct: 72  NNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAYG 131

Query: 124 EHWRNLRRIGAIEIFSASRLNKLAGIRKDEVERLLRKLSRNSLNGFSKVHMQSAISELTF 183
           + WRNLRRIG IEIFS+ RLN    IR+DE+ RL+  L++NS +GF KV M+     LT 
Sbjct: 132 DSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFMCLTI 191

Query: 184 NISMRMAAGKRYFGEEVTDEEEARQFGDLIKQVVALGGVSNPGDFIPMINWIPNGFKRKV 243
           N  +RM AGKR++G+   ++ EA+    LI +VV  GG  N  D+ P++ ++ N +++ V
Sbjct: 192 NNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYVTN-YEKHV 251

Query: 244 SGLAKRTDAFLQGLIEEHRSKKEEGRNTMIDHLLSLQESEPENYGDQIIKGIILLKMPQI 303
             LA R D FLQ L+ E R +K +G NTMIDHLLSLQE++P+ Y D IIKGIIL      
Sbjct: 252 KKLAGRVDEFLQSLVNEKRVEKVKG-NTMIDHLLSLQETQPDYYTDVIIKGIIL------ 311

Query: 304 FFINIVIIKRHVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDAQIGEDRLVEESD 363
                      V++LAGTDTSA T+EWA+++LLN+PEVL+KA+ E+D QIG DRLVEE D
Sbjct: 312 -----------VMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQD 371

Query: 364 VPKLPYLQGIISETLRLNPAAPMLVPHLTSDDCTISGYDIPRDTIVLVNAWAIHRDPNLW 423
           + KLPYLQ I+SETLRL P APML+PHL S+DC + GYD+PR TI+LVNAWAIHRDP LW
Sbjct: 372 IVKLPYLQHIVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLW 431

Query: 424 EEPTLFKPERHQKSSELGEHQVHKLVPFGVGRRACPGSGMAQRVVGLALAALIQCYEWQR 483
           EEP  FKPER +K  E       KL+PFG+GRR+CPGSG+AQR+V LAL +L+QC+EW+R
Sbjct: 432 EEPEKFKPERFEKKGE-----DKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWER 491

Query: 484 IGEEKIDMTEG-RGVTMPKAVPLEVMCKPRRIIHNLFN 515
           + E+ +DM E  +G TM KA  L+ MCK R I+H + +
Sbjct: 492 VEEKYLDMRESEKGTTMRKATSLQAMCKARPIVHKVLD 505

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038882511.11.5e-25989.30cytochrome P450 81Q32-like [Benincasa hispida][more]
XP_008440328.16.1e-24583.88PREDICTED: cytochrome P450 81E8 [Cucumis melo][more]
XP_011657880.21.4e-24483.88cytochrome P450 81Q32 [Cucumis sativus] >KAE8647508.1 hypothetical protein Csa_0... [more]
XP_022978934.14.5e-24081.32cytochrome P450 81E8-like [Cucurbita maxima][more]
XP_023543193.14.5e-24081.52cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
W8JMU73.2e-17260.04Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1[more]
Q6WNQ82.6e-16657.62Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1[more]
P931472.2e-16053.46Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2[more]
Q6WNQ91.9e-15652.80Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... [more]
Q6WNR01.6e-15551.94Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3B1G83.0e-24583.88cytochrome P450 81E8 OS=Cucumis melo OX=3656 GN=LOC103484810 PE=3 SV=1[more]
A0A0A0KJM04.7e-24383.50Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa_6G492230 PE=3 SV=1[more]
A0A6J1IVD62.2e-24081.32cytochrome P450 81E8-like OS=Cucurbita maxima OX=3661 GN=LOC111478736 PE=3 SV=1[more]
A0A6J1GGA43.8e-24081.32cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111453889 PE=3 SV=... [more]
A0A0A0KLF27.6e-23379.42Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G492220 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G37370.11.9e-15955.45cytochrome P450, family 81, subfamily D, polypeptide 8 [more]
AT4G37360.11.0e-15754.86cytochrome P450, family 81, subfamily D, polypeptide 2 [more]
AT4G37340.14.8e-15553.46cytochrome P450, family 81, subfamily D, polypeptide 3 [more]
AT4G37320.16.3e-15553.37cytochrome P450, family 81, subfamily D, polypeptide 5 [more]
AT3G28740.18.2e-15555.60Cytochrome P450 superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 56..75
score: 42.07
coord: 80..101
score: 19.44
coord: 302..319
score: 28.24
coord: 442..452
score: 54.13
coord: 322..348
score: 39.68
coord: 365..383
score: 34.32
coord: 175..193
score: 21.53
coord: 406..430
score: 33.65
coord: 452..475
score: 30.37
IPR001128Cytochrome P450PRINTSPR00385P450coord: 443..452
score: 57.25
coord: 452..463
score: 29.12
coord: 313..330
score: 38.2
coord: 366..377
score: 38.98
IPR001128Cytochrome P450PFAMPF00067p450coord: 29..495
e-value: 5.5E-89
score: 299.0
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 19..510
e-value: 6.2E-124
score: 416.2
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 29..506
NoneNo IPR availablePANTHERPTHR24298:SF836CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 2-RELATEDcoord: 4..511
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 4..511
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 445..454

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmUC01G016370.1CmUC01G016370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen