CmUC01G014600 (gene) Watermelon (USVL531) v1

Overview
NameCmUC01G014600
Typegene
OrganismCitrullus mucosospermus (Watermelon (USVL531) v1)
Descriptioncysteine proteinase inhibitor 6-like
LocationCmU531Chr01: 28014528 .. 28014890 (-)
RNA-Seq ExpressionCmUC01G014600
SyntenyCmUC01G014600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATTCGAGATAGTCGCCCCGGGACAAAACGATGCCTTGCTCCAAAACCGGTGCGAGCCAGGCGGTTGGGAACCGATCGAGAACATCCACGACTCGTACGTGCAAGAGATAGGAAGGTTTGCAGTGATGGAACACACACACAAGCTAGTAGGAGCATCACTGAGGTTCATTCGTGTGGTGAGTGGTGAGACTAGGCCGATGAGCGAAGGCAAAGAATACAGGCTTGTGGTGGAAGTGGCAGAACAGATAATAACTAGTCCTCCAATGTTATCAGTTTCTATCAAGTTTTACTTGGCAACTGTTCTGGAGAAGCCATTAGATCGATCTTGGACACTCAAAGCTTTTGTGCCTTGTAATTAG

mRNA sequence

ATGGAATTCGAGATAGTCGCCCCGGGACAAAACGATGCCTTGCTCCAAAACCGGTGCGAGCCAGGCGGTTGGGAACCGATCGAGAACATCCACGACTCGTACGTGCAAGAGATAGGAAGGTTTGCAGTGATGGAACACACACACAAGCTAGTAGGAGCATCACTGAGGTTCATTCGTGTGGTGAGTGGTGAGACTAGGCCGATGAGCGAAGGCAAAGAATACAGGCTTGTGGTGGAAGTGGCAGAACAGATAATAACTAGTCCTCCAATGTTATCAGTTTCTATCAAGTTTTACTTGGCAACTGTTCTGGAGAAGCCATTAGATCGATCTTGGACACTCAAAGCTTTTGTGCCTTGTAATTAG

Coding sequence (CDS)

ATGGAATTCGAGATAGTCGCCCCGGGACAAAACGATGCCTTGCTCCAAAACCGGTGCGAGCCAGGCGGTTGGGAACCGATCGAGAACATCCACGACTCGTACGTGCAAGAGATAGGAAGGTTTGCAGTGATGGAACACACACACAAGCTAGTAGGAGCATCACTGAGGTTCATTCGTGTGGTGAGTGGTGAGACTAGGCCGATGAGCGAAGGCAAAGAATACAGGCTTGTGGTGGAAGTGGCAGAACAGATAATAACTAGTCCTCCAATGTTATCAGTTTCTATCAAGTTTTACTTGGCAACTGTTCTGGAGAAGCCATTAGATCGATCTTGGACACTCAAAGCTTTTGTGCCTTGTAATTAG

Protein sequence

MEFEIVAPGQNDALLQNRCEPGGWEPIENIHDSYVQEIGRFAVMEHTHKLVGASLRFIRVVSGETRPMSEGKEYRLVVEVAEQIITSPPMLSVSIKFYLATVLEKPLDRSWTLKAFVPCN
Homology
BLAST of CmUC01G014600 vs. NCBI nr
Match: WP_198843152.1 (hypothetical protein, partial [Aureibaculum sp. A20] >MBJ2176574.1 hypothetical protein [Aureibaculum sp. A20])

HSP 1 Score: 222.6 bits (566), Expect = 1.8e-54
Identity = 103/120 (85.83%), Postives = 114/120 (95.00%), Query Frame = 0

Query: 1   MEFEIVAPGQNDALLQNRCEPGGWEPIENIHDSYVQEIGRFAVMEHTHKLVGASLRFIRV 60
           M+FE++APGQNDAL QNRC+PGGWEPI+NIHD YVQE+GRF+VMEHTHKLVGAS++FIRV
Sbjct: 5   MDFEVIAPGQNDALHQNRCKPGGWEPIKNIHDPYVQEMGRFSVMEHTHKLVGASMKFIRV 64

Query: 61  VSGETRPMSEGKEYRLVVEVAEQIITSPPMLSVSIKFYLATVLEKPLDRSWTLKAFVPCN 120
           VSGETRPMSEGKEYRLVVEVAE IIT PPMLSVS+KFYLATVLEKPLDRSW L+AFV CN
Sbjct: 65  VSGETRPMSEGKEYRLVVEVAEHIITCPPMLSVSVKFYLATVLEKPLDRSWQLEAFVSCN 124

BLAST of CmUC01G014600 vs. NCBI nr
Match: XP_038882290.1 (cysteine proteinase inhibitor 5-like [Benincasa hispida])

HSP 1 Score: 217.2 bits (552), Expect = 7.5e-53
Identity = 104/120 (86.67%), Postives = 113/120 (94.17%), Query Frame = 0

Query: 1   MEFEIVAPGQNDALLQNRCEPGGWEPIENIHDSYVQEIGRFAVMEHTHKLVGASLRFIRV 60
           M++E+VAPGQNDALLQNRCEPG WEPI+NIHD YVQE+GRFAVMEHTHKLVGASL+FI V
Sbjct: 1   MDYEVVAPGQNDALLQNRCEPGCWEPIKNIHDPYVQEMGRFAVMEHTHKLVGASLKFICV 60

Query: 61  VSGETRPMSEGKEYRLVVEVAEQIITSPPMLSVSIKFYLATVLEKPLDRSWTLKAFVPCN 120
           VSGETRPMS+GKEYRLVVEVAEQIITSPPMLSVSIKFYLATVLEKP+DRSW L +FV  N
Sbjct: 61  VSGETRPMSQGKEYRLVVEVAEQIITSPPMLSVSIKFYLATVLEKPIDRSWQLISFVSSN 120

BLAST of CmUC01G014600 vs. NCBI nr
Match: WP_156424223.1 (hypothetical protein, partial [Myroides odoratus])

HSP 1 Score: 204.5 bits (519), Expect = 5.0e-49
Identity = 95/108 (87.96%), Postives = 104/108 (96.30%), Query Frame = 0

Query: 4   EIVAPGQNDALLQNRCEPGGWEPIENIHDSYVQEIGRFAVMEHTHKLVGASLRFIRVVSG 63
           E++APGQNDAL QNRC+PGGWEPI+NIHD YVQE+GRF+VMEHTHKLVGAS++FIRVVSG
Sbjct: 1   EVIAPGQNDALHQNRCKPGGWEPIKNIHDPYVQEMGRFSVMEHTHKLVGASMKFIRVVSG 60

Query: 64  ETRPMSEGKEYRLVVEVAEQIITSPPMLSVSIKFYLATVLEKPLDRSW 112
           ETRPMSEGKEYRLVVEVAE IIT PPMLSVS+KFYLATVLEKPLDRSW
Sbjct: 61  ETRPMSEGKEYRLVVEVAEHIITCPPMLSVSVKFYLATVLEKPLDRSW 108

BLAST of CmUC01G014600 vs. NCBI nr
Match: XP_022132557.1 (cysteine proteinase inhibitor 6-like [Momordica charantia])

HSP 1 Score: 130.6 bits (327), Expect = 9.2e-27
Identity = 71/116 (61.21%), Postives = 87/116 (75.00%), Query Frame = 0

Query: 6   VAPG--QNDALLQNRCEPGGWEPIENIHDSYVQEIGRFAVMEHTHKLVGASLRFIRVVSG 65
           VAP   QNDALL N  +PGGWEPI+NI+D YVQE+GRFAVMEHTH L GAS  FIRVVSG
Sbjct: 6   VAPAVQQNDALL-NVQKPGGWEPIKNINDPYVQELGRFAVMEHTHNLCGASYEFIRVVSG 65

Query: 66  ETRPMSEGKEYRLVVEVAEQIITSPPMLSVSIKFYLATVLEKPLDRSWTLKAFVPC 120
           E++ +  GK+Y LV+EV E II   P +  SIKF+ A +L+K  ++SW L +FVPC
Sbjct: 66  ESQVVG-GKKYCLVIEVKETIII--PSMPSSIKFFKAIMLDKAWEKSWALLSFVPC 117

BLAST of CmUC01G014600 vs. NCBI nr
Match: KAG6604292.1 (putative cysteine proteinase inhibitor 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 121.3 bits (303), Expect = 5.6e-24
Identity = 64/118 (54.24%), Postives = 83/118 (70.34%), Query Frame = 0

Query: 1   MEFEIVAPGQNDALLQNRCEPGGWEPIENIHDSYVQEIGRFAVMEHTHKLVGASLRFIRV 60
           M+ ++VA GQNDALL ++ +   WEP+ N+HD YVQE+GRFAVMEH H L G  + FIRV
Sbjct: 1   MDLKVVAHGQNDALL-HKPKSCDWEPV-NVHDQYVQELGRFAVMEHVHGLCGVGMTFIRV 60

Query: 61  VSGETRPMSEGKEYRLVVEVAEQIITSPPMLSVSIKFYLATVLEKPLDRSWTLKAFVP 119
           VSGE++ + EGK+Y LVVEV E  +   P L  +IK Y+A VL+K   +SW L AF P
Sbjct: 61  VSGESKMVCEGKKYSLVVEVKE--VPVLPHLCPAIKLYVADVLDKQCAKSWELLAFKP 114

BLAST of CmUC01G014600 vs. ExPASy Swiss-Prot
Match: Q41916 (Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=2)

HSP 1 Score: 60.1 bits (144), Expect = 2.0e-08
Identity = 42/99 (42.42%), Postives = 53/99 (53.54%), Query Frame = 0

Query: 22  GGWEPIENIHDSYVQEIGRFAVMEHTHKLVGASLRFIRVVSGETRPMSEGKEYRLVVEVA 81
           GGW PI N+ D  V EIG FAV E+ +K   + L+F  VVSGET+ +S G  YRL V   
Sbjct: 29  GGWSPISNVTDPQVVEIGEFAVSEY-NKRSESGLKFETVVSGETQVVS-GTNYRLKVAAN 88

Query: 82  EQIITSPPMLSVSIKFYLATVLEKPLDRSWTLKAFVPCN 121
           +    S        K YLA V +KP  +   L +F P N
Sbjct: 89  DGDGVS--------KNYLAIVWDKPWMKFRNLTSFEPAN 117

BLAST of CmUC01G014600 vs. ExPASy Swiss-Prot
Match: Q10J94 (Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0429000 PE=2 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 6.5e-07
Identity = 37/97 (38.14%), Postives = 52/97 (53.61%), Query Frame = 0

Query: 22  GGWEPIENIHDSYVQEIGRFAVMEHTHKLVGASLRFIRVVSGETRPMSEGKEYRLVVEVA 81
           GGW PI ++ D ++QE+G +AV  H   L    LRF RV SGE + +S G  YRLVV  +
Sbjct: 33  GGWSPITDVGDPHIQELGGWAVERHA-SLSSDGLRFRRVTSGEQQVVS-GMNYRLVVSAS 92

Query: 82  EQIITSPPMLSVSIKFYLATVLEKPLDRSWTLKAFVP 119
           +     P   + S   Y+A V E+    +  L +F P
Sbjct: 93  D-----PAGATAS---YVAVVYEQSWTNTRQLTSFKP 119

BLAST of CmUC01G014600 vs. ExPASy Swiss-Prot
Match: Q10Q46 (Cysteine proteinase inhibitor 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210200 PE=3 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 8.5e-07
Identity = 27/61 (44.26%), Postives = 37/61 (60.66%), Query Frame = 0

Query: 21 PGGWEPIENIHDSYVQEIGRFAVMEHTHKLVGASLRFIRVVSGETRPMSEGKEYRLVVEV 80
          P GW PI+NI D ++QE+GR+A+ E+        L F RV  GE + +S G  YRL +E 
Sbjct: 20 PRGWSPIKNIDDPHIQELGRWAITENNRVSPSDELTFHRVTGGEQQVVS-GMNYRLEIEA 79

Query: 81 A 82
          A
Sbjct: 80 A 79

BLAST of CmUC01G014600 vs. ExPASy Swiss-Prot
Match: Q10Q47 (Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210100 PE=3 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 6.1e-05
Identity = 25/60 (41.67%), Postives = 37/60 (61.67%), Query Frame = 0

Query: 22 GGWEPIENIHDSYVQEIGRFAVMEHTHKLVGASLRFIRVVSGETRPMSEGKEYRLVVEVA 81
          G WEP++ I+D +VQE+GR+AV E    +    L F RV  GE + ++ G  YRL +E +
Sbjct: 28 GSWEPVD-INDPHVQELGRWAVAEEDRGVAAGGLTFERVTDGEKQVVA-GVNYRLTLEAS 85

BLAST of CmUC01G014600 vs. ExPASy TrEMBL
Match: A0A6J1BU61 (cysteine proteinase inhibitor 6-like OS=Momordica charantia OX=3673 GN=LOC111005388 PE=4 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 4.5e-27
Identity = 71/116 (61.21%), Postives = 87/116 (75.00%), Query Frame = 0

Query: 6   VAPG--QNDALLQNRCEPGGWEPIENIHDSYVQEIGRFAVMEHTHKLVGASLRFIRVVSG 65
           VAP   QNDALL N  +PGGWEPI+NI+D YVQE+GRFAVMEHTH L GAS  FIRVVSG
Sbjct: 6   VAPAVQQNDALL-NVQKPGGWEPIKNINDPYVQELGRFAVMEHTHNLCGASYEFIRVVSG 65

Query: 66  ETRPMSEGKEYRLVVEVAEQIITSPPMLSVSIKFYLATVLEKPLDRSWTLKAFVPC 120
           E++ +  GK+Y LV+EV E II   P +  SIKF+ A +L+K  ++SW L +FVPC
Sbjct: 66  ESQVVG-GKKYCLVIEVKETIII--PSMPSSIKFFKAIMLDKAWEKSWALLSFVPC 117

BLAST of CmUC01G014600 vs. ExPASy TrEMBL
Match: A0A6J1BX23 (cysteine proteinase inhibitor 5-like OS=Momordica charantia OX=3673 GN=LOC111005501 PE=4 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 6.7e-23
Identity = 69/122 (56.56%), Postives = 87/122 (71.31%), Query Frame = 0

Query: 1   MEFEIVAPGQNDALLQNRCEPGGWEPIENIHDSYVQEIGRFAVMEHTHKLVG--ASLRFI 60
           M+   VAP QNDA LQ+  +PGGWEPI+NI D YVQE+GRFAVME   K VG  AS+ F+
Sbjct: 32  MDSVAVAPAQNDA-LQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQP-KPVGLVASMEFV 91

Query: 61  RVVSGETRPMSEGKEYRLVVE--VAEQIITSPPMLSVSIKFYLATVLEKPLDRSWTLKAF 119
            VVSGE + ++ GKEYRLVVE  V E+II     +S SI+ Y+A VL+KP D+SW + +F
Sbjct: 92  GVVSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVS-SIRLYVAVVLDKPSDKSWKVLSF 150

BLAST of CmUC01G014600 vs. ExPASy TrEMBL
Match: A0A6J1BUK6 (cysteine proteinase inhibitor 5-like OS=Momordica charantia OX=3673 GN=LOC111005500 PE=4 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 8.7e-23
Identity = 68/122 (55.74%), Postives = 85/122 (69.67%), Query Frame = 0

Query: 1   MEFEIVAPGQNDALLQNRCEPGGWEPIENIHDSYVQEIGRFAVMEHTHK--LVGASLRFI 60
           M+   VAP QNDALL  +  PGGWEPI+NI D YVQE+GRFAVME   K   + AS+ FI
Sbjct: 1   MDSVAVAPAQNDALLHLKM-PGGWEPIKNIKDPYVQEMGRFAVMEQHPKPSELIASMEFI 60

Query: 61  RVVSGETRPMSEGKEYRLVVE--VAEQIITSPPMLSVSIKFYLATVLEKPLDRSWTLKAF 119
            VVSGE + ++ GKEYRLVVE  V E+II     +S SI+ Y+A VL+KP D+SW + +F
Sbjct: 61  GVVSGEIQLVAHGKEYRLVVEVRVTEKIIVHGCPVS-SIRLYVAVVLDKPSDKSWKVLSF 120

BLAST of CmUC01G014600 vs. ExPASy TrEMBL
Match: A0A6J1GCT2 (uncharacterized protein LOC111453027 OS=Cucurbita moschata OX=3662 GN=LOC111453027 PE=4 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 8.7e-23
Identity = 62/118 (52.54%), Postives = 81/118 (68.64%), Query Frame = 0

Query: 1   MEFEIVAPGQNDALLQNRCEPGGWEPIENIHDSYVQEIGRFAVMEHTHKLVGASLRFIRV 60
           M+ ++VA GQNDALL ++ +   WEP+ N+HD YVQE+GRFAVMEH H L G  + FI V
Sbjct: 1   MDLKVVAHGQNDALL-HKPKSCDWEPV-NVHDQYVQELGRFAVMEHVHGLCGVGMTFICV 60

Query: 61  VSGETRPMSEGKEYRLVVEVAEQIITSPPMLSVSIKFYLATVLEKPLDRSWTLKAFVP 119
           VSGE++ + EGK+Y LVVEV E  +   P    +IK Y+A VL+K   +SW L AF P
Sbjct: 61  VSGESKMVCEGKKYSLVVEVKE--VPVLPHACPAIKLYVADVLDKQCAKSWELLAFKP 114

BLAST of CmUC01G014600 vs. ExPASy TrEMBL
Match: A0A6J1FCN5 (cysteine proteinase inhibitor 8-like OS=Cucurbita moschata OX=3662 GN=LOC111442890 PE=4 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 5.5e-17
Identity = 51/114 (44.74%), Postives = 74/114 (64.91%), Query Frame = 0

Query: 7   APGQNDALLQNR--CEPGGWEPIENIHDSYVQEIGRFAVMEHTHKLVGASLRFIRVVSGE 66
           APGQN+A+ Q++   E G W+PIE+I D YVQE+GRFAVMEH +  +G +L+FI V SGE
Sbjct: 8   APGQNEAMFQDKKGIEVGQWKPIEDIKDPYVQEMGRFAVMEHDNMRIGENLKFICVQSGE 67

Query: 67  TRPMSEGKEYRLVVEVAEQIITSPPMLSVSIKFYLATVLEKPLDRSWTLKAFVP 119
            + + EG  YRL+++ +  +  S          Y+A V +KP +++W L  FVP
Sbjct: 68  -KQVVEGMNYRLLIKASNHLGIS--------SLYMAIVYDKPWEKTWKLIEFVP 112

BLAST of CmUC01G014600 vs. TAIR 10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 60.1 bits (144), Expect = 1.4e-09
Identity = 42/99 (42.42%), Postives = 53/99 (53.54%), Query Frame = 0

Query: 22  GGWEPIENIHDSYVQEIGRFAVMEHTHKLVGASLRFIRVVSGETRPMSEGKEYRLVVEVA 81
           GGW PI N+ D  V EIG FAV E+ +K   + L+F  VVSGET+ +S G  YRL V   
Sbjct: 29  GGWSPISNVTDPQVVEIGEFAVSEY-NKRSESGLKFETVVSGETQVVS-GTNYRLKVAAN 88

Query: 82  EQIITSPPMLSVSIKFYLATVLEKPLDRSWTLKAFVPCN 121
           +    S        K YLA V +KP  +   L +F P N
Sbjct: 89  DGDGVS--------KNYLAIVWDKPWMKFRNLTSFEPAN 117

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
WP_198843152.11.8e-5485.83hypothetical protein, partial [Aureibaculum sp. A20] >MBJ2176574.1 hypothetical ... [more]
XP_038882290.17.5e-5386.67cysteine proteinase inhibitor 5-like [Benincasa hispida][more]
WP_156424223.15.0e-4987.96hypothetical protein, partial [Myroides odoratus][more]
XP_022132557.19.2e-2761.21cysteine proteinase inhibitor 6-like [Momordica charantia][more]
KAG6604292.15.6e-2454.24putative cysteine proteinase inhibitor 7, partial [Cucurbita argyrosperma subsp.... [more]
Match NameE-valueIdentityDescription
Q419162.0e-0842.42Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=... [more]
Q10J946.5e-0738.14Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03... [more]
Q10Q468.5e-0744.26Cysteine proteinase inhibitor 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03... [more]
Q10Q476.1e-0541.67Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=3994... [more]
Match NameE-valueIdentityDescription
A0A6J1BU614.5e-2761.21cysteine proteinase inhibitor 6-like OS=Momordica charantia OX=3673 GN=LOC111005... [more]
A0A6J1BX236.7e-2356.56cysteine proteinase inhibitor 5-like OS=Momordica charantia OX=3673 GN=LOC111005... [more]
A0A6J1BUK68.7e-2355.74cysteine proteinase inhibitor 5-like OS=Momordica charantia OX=3673 GN=LOC111005... [more]
A0A6J1GCT28.7e-2352.54uncharacterized protein LOC111453027 OS=Cucurbita moschata OX=3662 GN=LOC1114530... [more]
A0A6J1FCN55.5e-1744.74cysteine proteinase inhibitor 8-like OS=Cucurbita moschata OX=3662 GN=LOC1114428... [more]
Match NameE-valueIdentityDescription
AT5G47550.11.4e-0942.42Cystatin/monellin superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.10.450.10coord: 18..119
e-value: 5.2E-17
score: 63.8
NoneNo IPR availableSUPERFAMILY54403Cystatin/monellincoord: 22..118
IPR000010Cystatin domainPFAMPF16845SQAPIcoord: 27..117
e-value: 3.2E-12
score: 46.6
IPR000010Cystatin domainCDDcd00042CYcoord: 22..106
e-value: 3.17567E-7
score: 43.6168
IPR027214CystatinPANTHERPTHR47116PHLOEM FILAMENT PROTEINcoord: 19..118

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmUC01G014600.1CmUC01G014600.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010466 negative regulation of peptidase activity
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity