CmUC01G000460 (gene) Watermelon (USVL531) v1

Overview
NameCmUC01G000460
Typegene
OrganismCitrullus mucosospermus (Watermelon (USVL531) v1)
DescriptionPectinesterase
LocationCmU531Chr01: 314584 .. 320529 (-)
RNA-Seq ExpressionCmUC01G000460
SyntenyCmUC01G000460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAAAAAGAGGTGGATGTAGGTTAACATTACCCTTAATATAAGGAACATATACTGAAATAATGGAGGCATGGAAGGTAGCAAGATCACTTCCCCCACAAAACATACACGTATTGATACACAATAAAAGAGATTATTGAGTAGAAAGATTTAGAAGGACAAGAAAGGGCCAACCCCACATTATAATTAGAATCATATTGTGGAATAATGTTGGCCAACTTTTTGTTATTTAAACTCACTCACTTCCATTATTCCCTCCCCAACTCCATGCACACTCATTATTCTATCTTCTTCAACCATGATCAACAACAACTACAACAAGCTTCACTCCTCCCGCTCCCTCGACTCTACTCCCGCCACCTCCTTCCACCTTTCCCCCAATGATCCTCCGCCACCTCCACCCTCCTCCTCCCTCCGCCGCCGCTCTCGTATCCTTATCATCTCCTTCCTCTCCGTCCTCCTCATCGCTGCCTCCGTGGTCTCCGCCGCCATGCTCGTCCGGTCCCATATCGCATCGAGCCAACCCAATTTCCCTCACAACAAGCCGTCCCAAGCCATCTCCCGAGCCTGCAGCCACACTCTGTACCCATCTCTCTGCCTCTCTTCTCTTCTCTCCTTCCCCGGCTCTCTCTCCGCCGACGTCCATGACCTTGTTCACATCTCTCTCAACTTAACTCTTCAACATCTCACCAAAGCTCTTTATTCCACTTCTCAAATCCCCATCTCCCAAATCTCCAACGACCCTTTAGCCCATTCCGCTTATGAAGACTGCATGGAGCTTCTAAACGACGCCGTTGACGCTTTTTCCCTCTCTCTCTTTTCCACTGAAGCTTCTAATCACGACGTCATGACGTGGCTTAGTGCGGCATTGACTTATCATGACACGTGTACGGATGGATTTGCGGATGTGGCTGACTTGGCAGTTAAGGATCAGGTGGAGGCGAAGTTGAGTGATTTGTCGGAGATGATTAGTAATTCTTTGGCTATATTTTCTGGGTTTGGTGGTGGGGATTTGCCGGTGGAGAATAGGCGCCGCCGCCGGTTGATGGAGAGTGGTACACAGCCGTGGGCGGCGGAGAATGGTGGTGATGGGGGTTTTCCACAGTGGGTGAGTGGGAAGGATCGGAGGCTTTTGGCTACTCCTATTTCGGCGATTCAGGCGGATATTGTGGTGGCTAAGGATGGTTCTGGCAAATTCAAGACGGTGGCTGAGGCCATTGACGCGGCTCCGTCGTCGAGTGGCCGCCGGATTGTTATCTACATTAAGGCCGGAAAGTAAGAACAATACTTTTCTCTACTCATTCAAAGTGAGAGTATACATATTTTTTCACTATTTAAAACATAGTTTTTTTTTAATATTTACCTACCAAAATTATTCCTACTTATACCATGCTTCTACAACCATCCAAATAATCAATCACAAGTTGACACATGACAACAAATTTTTATTTTAAAAAGATAATTTTATTTATTTATTTATTTTTAATGGAAAGAAATGTTTAGGATGGACGCAGACTGAACTACATTATTATTTTTTACCAACCGACCTCTCTTTCTTTTAAGGAAAAAAAATTATAAACCTATTTTTGCCTTTGATGAATTTTTTTTAAAAAGAAATGTAATAGGTGGATTTCAAAATTTTAGTTTTAAGAAGGTAAAAATCTATTTCTGAAAAAAAAAAAAAAGGATAGAAATCTAGGGGTCCAAGTTGGTTGGATTATTAGAGAGTGGATCATGATCTTTCATTATTTTTCTTTTTTTCAAAATATGAATGATTGTTAATTTATTCCTTTTTTTTTTCCTTGGGAAAGTGGAGAAATAAAATGCTTGTGATTAGGTCATAATCAATCAACATTAACTCTCATTAAAAGGAAGGAAAGAAAGGGGAAAAAAAAGTTTATTTGCGCAACTTTTTCCTGATGTGTTTAATTTATTGACTTCAGTGTCATAATCATGCAAAGCTGTTATTTACCAATTCCTAAAGTCCATGGTTTTCATGATAATAACTATACAATCAAAACAATATTATATGTCCCCTCCCACTAATTTAAGCCCTTTTCAATTTAGTTGCTTTCATGATAATAAATCTAATAACAACTAAAATAAAAACATTAAAATTTATCAAAAAGAGCATAATTCAATTGGTATAATATTTATATTATAATATCGCAGTCAAAAAGTATAAAAATTAATCTATAGTAAAATGCTATGATACAGACTAACTACGAATATGAAAATAGTATAGAAAGTGATACATCAAATCAATAAAATACAAGTTAGTAGAAAGGGATTGGTCACTACAATGATCGATCACGAGAATTATAGTATATTTAAAGGCCAACACTTATAGGATTTTTTTTTTTTTTTTTTTTTTTATAATAATTGAAATGGTGTGGGACTAAGAAGGGAGATTAACGTAATTTTGTAGGTACGAAGAGGAGAATTTGAAGGTGGGAAGGAAGAAAACAAACTTGATGTTTGTAGGAGATGGAAAGGGCATAACGGTCATTTCAGGTGGCAAAAGTATCTTTGACAAAGTTACCACTTTTCGCACCGCCACCTTTGGTATATACTCTCACTCTTTCCCCCTTTTTCAATTTTATTCCAAAACCTTTTAAAAAGATATAAAAAATCTTTTGACATTAATAACTAAATAAATAAAATATTATTTCGATCTGTATAGTTTTTTTAATTTTAGTATTTATATTTTTAAATATTTAATTTTAGTCTTGTAATTACAATAATAATAATCTTAAATCCAGTTTTTCAAAATATTTTTTTATTAAAATTGATTAAGGGATAATAATCCTTATGTAAATATATTTCTGATATTTCTAAAATTTATAGTGCAAATACTAGTAATAAAAAGTTGGTTATAATGACATTTTGATCCCTTTTATTCTGGAGTGTTCAATATGCTTTCAAATGTCTAATTTTAGTCTATATATATTTAATAAATCTTAAATTAAGTCCTCAAATTTAATTTTATAATCATAATAATTTTATGCTAAGAAATATAATAAGTTAATATTTCTTCAAATTTATAGTAAAAATATTAATAAATTATTTTTTTAAAAAAAAAAAGCAATAACAAACTAGCTAGTAATATAAATTCAATTTAAAATTGATCGAAAATAATAAAATTAAAATTGAATATTTGAAAATACGGGACTAAAGATGAAACAATTTTAACATAGAGAAAAATAATATTTTAATTTAAAATATATATTTCTAAAAAGATAACCAATCTTTTAGTTCTGACACCGGATATCTAGGACTTAGAATTATGGTCGTCCGGTCAGAACCAAATGTGTCCGTACTTTTCCTTTTCCTTTTTCTTTTTCTTTTTCTTTTTCTACTTGAACTTCATTTATACTCTCATGGTTAGTCTTATAATTTTTTTTTAATTGTATTTATAATCTCGTAATTTTATTGCAATAATTTCTATATTTTTTTAAATAAACATTTGAAAAATATAATTTTAAAAACTATCTTTTTCAAAACTTTGCTTGAATTTTTAAAGAAAATTATATATATTATTTTTAGAAAATTTGAATTACCTATGTAACTTGTTAATACACATAATAATGTAGATTTTTTGGAATGGATATTTGGTAAGAATATTTTAGGTGGTAGAATAATAGCAAGTATTTAATATGAGCAATACAGTTTAGTGATTACGCTGACATCTTTATTTTTCTATTAGGTAGTAAGTTTAAATCCTTGCATTTATTCTAAAAAATTAGTATCAATTTTAAAATATATATATAGAAAGGTTAGGACCAATGATTTATTTTCTTTTTTGAGAAAATAATTATTACACAATTTAAAAGAAAAAAAAAAAACAATGAATTCCTGTACCATTTCTCCCCTTTAGATACATATATTTTTATGGTAGAGAAAAAAAAATTAGAGGCTACAAGTTTTTGTTAAAAATAATTATATTTAAAGATAGTGACAAATTAAATTAAGCTACTTTTGTAGCATGGAATGGGCTACAAAAATAATTATTTGATTTATCCAAATAATCATATGATAAATAGTAGATTCCAGCTGTGTTTGGCAAGTAGCCTTCTTTCTTATATGCAATCATTAATTATTTTTGAGGAAAAATGCCCACCATTCATATTTTATAATTATAAGGCTGTATGCTAATGGCTACATTTACCAATAAAGTCAAGCTTACAAAATTGAATATTTGACGTAACATTTTCACATGACATATTATAGACATATATTATAAACATTTTCGTCTTCCTACTGATATTATAAAAAGTTGATCATCATTAAAAAAAAAATATAAAATAAAAAAAAATAAAAAAAATACTAATTAAGCAAGTTATTTCGATTTTTTAATACAATTGAGATAAGGGAGTTTGAACTATTGATCTGCCAATTGAATTATGTTTGGTTTTATTGATTTCAATAGTTTCAAAAATCTTTTCATTATCTTTATAAAAAAAGATGATGTCTCTGTTAAATAGTATATTGTGTCCATCATAATTAGTTAGTTAACAAAATTGAGAATTATTATGACTCAAAATATTTTAGAATAATTAAATTAAAAAAAAAAAAAGAATGGAATATCCCAATGTTTCATGATAATTTTGAAAAGTCATAGTAGTATTATTCCTTGTTCTTGCAACAACTTTCTTCCACAACTTGGCAAAATAGTTTTTTTTTAAAAAAAAAAAAAATTAATTTTTAATATTTAATTTAAGAATCTAATGTGGTCCAAATAAAGATGCCAAAAGTTTACTCCAAGGAGATAAGGATAGAAGTTAGAAAGTATTGAGTTTTATTTCAAAATTGCTCTATATTTCATGACACAATTCATGGTTCTCACCTATAAATTTATAGAAGTAATTGATTTTCTTTGGTTGGTAAATTATGAATTTAGCTTCTATATTTTTATCTTTAAATGATTGTGTTAATGGTTTCTTAAACTTAATATTACAGCTGAGAATTAAAGACTTAGGATTAATAGGTGTTTAGATTTTTGAATTTTCAATGGTGTTTAAAAGGTTATAAATTTTTGTAATATTGCGCAGCGGGTTCGGGAACAGGAATAATTTTAAGAGACATGACGTTTGAGAACACGGCAGGAGCAAGCAAGCACCAAGCAGTGGCATTGCGCTTGAGCGCTGACCACGCTGTGGTATACCGTTGCAACATCATTGGTTATCAAGACACTCTCTATGTGCACTCAAACCGCCAATTCTTTCGAGAATGCGACATCTACGGTACGGTGGACTTCATCTTTGGGAATGCGGTAGTTGTGTTCCAAAGTTGCAACATCTATGCCCGAAAGCCAATGGCGGGGCAAAAGAACACCATCACTGCCCAAGACCGGAAGGACCCAAACCAAAACACTGGAATCTCCATCCATGCTTGTAAGATCATTGCCACTAGCGACCTCGAAGCCTCCAAGGGTTCTTTCCCCACCTACCTAGGTCGTCCTTGGAAATTATACTCTAGAGTGGTGTACATGGTATCTTCCATGGGCGACCATATTCATCCACGTGGTTGGCTGGAGTGGCAAGGTTCTTTTGCTCTCGACACTCTTTACTACGGCGAGTATATGAATTCTGGGCCAGGTGCCGCTGTGGGGCAGCGGGTCAAGTGGCCGGGTTATCGGGTTATAACTTCCACGGTAGAAGCCTCCAAATTTACTGTTGGACAATTCATTTATGGCTCTTCTTGGTTGCCCTCCACTGGAGTCTCCTTTTCAGCTGGATTATCCGCTAACTAAAATGCATTTTAATTCACTTTACTTTCAAGTCTGTATTTAAATAATTAATATTGTTTTTTTTTTTTTTTTTTTTTTAGGATGTGGTTGGATGATGACACATGTATAGTATCATTCCTTTTATTATGCTCTTCCAAAATAATATGAGCCACACATCAAACTATGAGGGTATTTGGATGATCATATATATACACACACA

mRNA sequence

ATAAAAAGAGGTGGATGTAGGTTAACATTACCCTTAATATAAGGAACATATACTGAAATAATGGAGGCATGGAAGGTAGCAAGATCACTTCCCCCACAAAACATACACGTATTGATACACAATAAAAGAGATTATTGAGTAGAAAGATTTAGAAGGACAAGAAAGGGCCAACCCCACATTATAATTAGAATCATATTGTGGAATAATGTTGGCCAACTTTTTGTTATTTAAACTCACTCACTTCCATTATTCCCTCCCCAACTCCATGCACACTCATTATTCTATCTTCTTCAACCATGATCAACAACAACTACAACAAGCTTCACTCCTCCCGCTCCCTCGACTCTACTCCCGCCACCTCCTTCCACCTTTCCCCCAATGATCCTCCGCCACCTCCACCCTCCTCCTCCCTCCGCCGCCGCTCTCGTATCCTTATCATCTCCTTCCTCTCCGTCCTCCTCATCGCTGCCTCCGTGGTCTCCGCCGCCATGCTCGTCCGGTCCCATATCGCATCGAGCCAACCCAATTTCCCTCACAACAAGCCGTCCCAAGCCATCTCCCGAGCCTGCAGCCACACTCTGTACCCATCTCTCTGCCTCTCTTCTCTTCTCTCCTTCCCCGGCTCTCTCTCCGCCGACGTCCATGACCTTGTTCACATCTCTCTCAACTTAACTCTTCAACATCTCACCAAAGCTCTTTATTCCACTTCTCAAATCCCCATCTCCCAAATCTCCAACGACCCTTTAGCCCATTCCGCTTATGAAGACTGCATGGAGCTTCTAAACGACGCCGTTGACGCTTTTTCCCTCTCTCTCTTTTCCACTGAAGCTTCTAATCACGACGTCATGACGTGGCTTAGTGCGGCATTGACTTATCATGACACGTGTACGGATGGATTTGCGGATGTGGCTGACTTGGCAGTTAAGGATCAGGTGGAGGCGAAGTTGAGTGATTTGTCGGAGATGATTAGTAATTCTTTGGCTATATTTTCTGGGTTTGGTGGTGGGGATTTGCCGGTGGAGAATAGGCGCCGCCGCCGGTTGATGGAGAGTGGTACACAGCCGTGGGCGGCGGAGAATGGTGGTGATGGGGGTTTTCCACAGTGGGTGAGTGGGAAGGATCGGAGGCTTTTGGCTACTCCTATTTCGGCGATTCAGGCGGATATTGTGGTGGCTAAGGATGGTTCTGGCAAATTCAAGACGGTGGCTGAGGCCATTGACGCGGCTCCGTCGTCGAGTGGCCGCCGGATTGTTATCTACATTAAGGCCGGAAAGTACGAAGAGGAGAATTTGAAGGTGGGAAGGAAGAAAACAAACTTGATGTTTGTAGGAGATGGAAAGGGCATAACGGTCATTTCAGGTGGCAAAAGTATCTTTGACAAAGTTACCACTTTTCGCACCGCCACCTTTGCGGGTTCGGGAACAGGAATAATTTTAAGAGACATGACGTTTGAGAACACGGCAGGAGCAAGCAAGCACCAAGCAGTGGCATTGCGCTTGAGCGCTGACCACGCTGTGGTATACCGTTGCAACATCATTGGTTATCAAGACACTCTCTATGTGCACTCAAACCGCCAATTCTTTCGAGAATGCGACATCTACGGTACGGTGGACTTCATCTTTGGGAATGCGGTAGTTGTGTTCCAAAGTTGCAACATCTATGCCCGAAAGCCAATGGCGGGGCAAAAGAACACCATCACTGCCCAAGACCGGAAGGACCCAAACCAAAACACTGGAATCTCCATCCATGCTTGTAAGATCATTGCCACTAGCGACCTCGAAGCCTCCAAGGGTTCTTTCCCCACCTACCTAGGTCGTCCTTGGAAATTATACTCTAGAGTGGTGTACATGGTATCTTCCATGGGCGACCATATTCATCCACGTGGTTGGCTGGAGTGGCAAGGTTCTTTTGCTCTCGACACTCTTTACTACGGCGAGTATATGAATTCTGGGCCAGGTGCCGCTGTGGGGCAGCGGGTCAAGTGGCCGGGTTATCGGGTTATAACTTCCACGGTAGAAGCCTCCAAATTTACTGTTGGACAATTCATTTATGGCTCTTCTTGGTTGCCCTCCACTGGAGTCTCCTTTTCAGCTGGATTATCCGCTAACTAAAATGCATTTTAATTCACTTTACTTTCAAGTCTGTATTTAAATAATTAATATTGTTTTTTTTTTTTTTTTTTTTTTAGGATGTGGTTGGATGATGACACATGTATAGTATCATTCCTTTTATTATGCTCTTCCAAAATAATATGAGCCACACATCAAACTATGAGGGTATTTGGATGATCATATATATACACACACA

Coding sequence (CDS)

ATGATCAACAACAACTACAACAAGCTTCACTCCTCCCGCTCCCTCGACTCTACTCCCGCCACCTCCTTCCACCTTTCCCCCAATGATCCTCCGCCACCTCCACCCTCCTCCTCCCTCCGCCGCCGCTCTCGTATCCTTATCATCTCCTTCCTCTCCGTCCTCCTCATCGCTGCCTCCGTGGTCTCCGCCGCCATGCTCGTCCGGTCCCATATCGCATCGAGCCAACCCAATTTCCCTCACAACAAGCCGTCCCAAGCCATCTCCCGAGCCTGCAGCCACACTCTGTACCCATCTCTCTGCCTCTCTTCTCTTCTCTCCTTCCCCGGCTCTCTCTCCGCCGACGTCCATGACCTTGTTCACATCTCTCTCAACTTAACTCTTCAACATCTCACCAAAGCTCTTTATTCCACTTCTCAAATCCCCATCTCCCAAATCTCCAACGACCCTTTAGCCCATTCCGCTTATGAAGACTGCATGGAGCTTCTAAACGACGCCGTTGACGCTTTTTCCCTCTCTCTCTTTTCCACTGAAGCTTCTAATCACGACGTCATGACGTGGCTTAGTGCGGCATTGACTTATCATGACACGTGTACGGATGGATTTGCGGATGTGGCTGACTTGGCAGTTAAGGATCAGGTGGAGGCGAAGTTGAGTGATTTGTCGGAGATGATTAGTAATTCTTTGGCTATATTTTCTGGGTTTGGTGGTGGGGATTTGCCGGTGGAGAATAGGCGCCGCCGCCGGTTGATGGAGAGTGGTACACAGCCGTGGGCGGCGGAGAATGGTGGTGATGGGGGTTTTCCACAGTGGGTGAGTGGGAAGGATCGGAGGCTTTTGGCTACTCCTATTTCGGCGATTCAGGCGGATATTGTGGTGGCTAAGGATGGTTCTGGCAAATTCAAGACGGTGGCTGAGGCCATTGACGCGGCTCCGTCGTCGAGTGGCCGCCGGATTGTTATCTACATTAAGGCCGGAAAGTACGAAGAGGAGAATTTGAAGGTGGGAAGGAAGAAAACAAACTTGATGTTTGTAGGAGATGGAAAGGGCATAACGGTCATTTCAGGTGGCAAAAGTATCTTTGACAAAGTTACCACTTTTCGCACCGCCACCTTTGCGGGTTCGGGAACAGGAATAATTTTAAGAGACATGACGTTTGAGAACACGGCAGGAGCAAGCAAGCACCAAGCAGTGGCATTGCGCTTGAGCGCTGACCACGCTGTGGTATACCGTTGCAACATCATTGGTTATCAAGACACTCTCTATGTGCACTCAAACCGCCAATTCTTTCGAGAATGCGACATCTACGGTACGGTGGACTTCATCTTTGGGAATGCGGTAGTTGTGTTCCAAAGTTGCAACATCTATGCCCGAAAGCCAATGGCGGGGCAAAAGAACACCATCACTGCCCAAGACCGGAAGGACCCAAACCAAAACACTGGAATCTCCATCCATGCTTGTAAGATCATTGCCACTAGCGACCTCGAAGCCTCCAAGGGTTCTTTCCCCACCTACCTAGGTCGTCCTTGGAAATTATACTCTAGAGTGGTGTACATGGTATCTTCCATGGGCGACCATATTCATCCACGTGGTTGGCTGGAGTGGCAAGGTTCTTTTGCTCTCGACACTCTTTACTACGGCGAGTATATGAATTCTGGGCCAGGTGCCGCTGTGGGGCAGCGGGTCAAGTGGCCGGGTTATCGGGTTATAACTTCCACGGTAGAAGCCTCCAAATTTACTGTTGGACAATTCATTTATGGCTCTTCTTGGTTGCCCTCCACTGGAGTCTCCTTTTCAGCTGGATTATCCGCTAACTAA

Protein sequence

MINNNYNKLHSSRSLDSTPATSFHLSPNDPPPPPPSSSLRRRSRILIISFLSVLLIAASVVSAAMLVRSHIASSQPNFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGSLSADVHDLVHISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSLSLFSTEASNHDVMTWLSAALTYHDTCTDGFADVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRRRRRLMESGTQPWAAENGGDGGFPQWVSGKDRRLLATPISAIQADIVVAKDGSGKFKTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIFDKVTTFRTATFAGSGTGIILRDMTFENTAGASKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIIATSDLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLSAN
Homology
BLAST of CmUC01G000460 vs. NCBI nr
Match: XP_038874841.1 (probable pectinesterase/pectinesterase inhibitor 61 [Benincasa hispida])

HSP 1 Score: 1116.3 bits (2886), Expect = 0.0e+00
Identity = 570/605 (94.21%), Postives = 585/605 (96.69%), Query Frame = 0

Query: 1   MINNNYNKLHSSRSLDSTPATSFHLSPND-PPPPPPSSSLRRRSRILIISFLSVLLIAAS 60
           MINNNYNKLHSS S  STPA+SFHLS ND PP PPPSSS RRRSRILIISFLSVLLIAAS
Sbjct: 1   MINNNYNKLHSSLSATSTPASSFHLSGNDLPPTPPPSSSHRRRSRILIISFLSVLLIAAS 60

Query: 61  VVSAAMLVRSHIASSQPNFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGSLSADVHDLV 120
           +VSAA+LVRS IAS++P+FPHNKPSQAISRACSHTLYPSLCLSSLLS P +LSADVHDLV
Sbjct: 61  IVSAAVLVRSRIASNEPHFPHNKPSQAISRACSHTLYPSLCLSSLLSIPDALSADVHDLV 120

Query: 121 HISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSLSLFSTEAS 180
           HISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFS+SLFSTEAS
Sbjct: 121 HISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSVSLFSTEAS 180

Query: 181 NHDVMTWLSAALTYHDTCTDGFADVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDL 240
           NHDVMTWLSAALTYHDTCTDGFADVADLAVK+QVEAKLSDLSEMISNSLAIFSGFGGGDL
Sbjct: 181 NHDVMTWLSAALTYHDTCTDGFADVADLAVKNQVEAKLSDLSEMISNSLAIFSGFGGGDL 240

Query: 241 PVENRRRRRLMESGTQPWAAENGGDGGFPQWVSGKDRRLLATPISAIQADIVVAKDGSGK 300
           PV+N RRRRLMESGT+ WAAENGGDGGFPQW+S KDRRLLA PISAIQADIVVAKDGSGK
Sbjct: 241 PVQN-RRRRLMESGTRSWAAENGGDGGFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGK 300

Query: 301 FKTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSI 360
           FKTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKS+
Sbjct: 301 FKTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 360

Query: 361 FDKVTTFRTATFAGSGTGIILRDMTFENTAGASKHQAVALRLSADHAVVYRCNIIGYQDT 420
           FDKVTTFRTATFAGSGTGIILRDM FENTAG SKHQAVALRLSADHAVVYRCNIIGYQDT
Sbjct: 361 FDKVTTFRTATFAGSGTGIILRDMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 420

Query: 421 LYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNT 480
           LYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPM GQKNTITAQDRKDPNQNT
Sbjct: 421 LYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNT 480

Query: 481 GISIHACKIIATSDLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540
           GISIHACKIIATSDL+ASKGSFPT+LGRPWKLYSRVVYMVS MGDHIHPRGWLEWQGSFA
Sbjct: 481 GISIHACKIIATSDLQASKGSFPTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFA 540

Query: 541 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 600
           LDTLYYGEY NSGPGAAVGQRVKW GYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA
Sbjct: 541 LDTLYYGEYSNSGPGAAVGQRVKWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 600

Query: 601 GLSAN 605
           GLSAN
Sbjct: 601 GLSAN 604

BLAST of CmUC01G000460 vs. NCBI nr
Match: XP_011654619.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucumis sativus] >KGN49865.1 hypothetical protein Csa_000423 [Cucumis sativus])

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 551/603 (91.38%), Postives = 575/603 (95.36%), Query Frame = 0

Query: 3   NNNYNKLHSSRSLDSTPATSFHLSPNDPPPPPPSSSLRRRSRILIISFLSVLLIAASVVS 62
           NN+YNKLH+S S ++TPA SF LS ND  PPPPSSSLRRRSRILIISF+SVLLIAASVVS
Sbjct: 4   NNSYNKLHTSHSANTTPAASFRLSGND--PPPPSSSLRRRSRILIISFMSVLLIAASVVS 63

Query: 63  AAMLVRSHIASSQPNFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGSLSADVHDLVHIS 122
           AA+LVRSH+ SSQP+FPHNKPSQAISRACSHTLYPSLCLSSLLSFPG+ SADVHDLVHIS
Sbjct: 64  AAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHIS 123

Query: 123 LNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSLSLFSTEASNHD 182
           LNLTLQHLTKALYSTSQIP+ QIS DPLAHSAYEDCMELLNDA+DAFSLSLFS +ASNHD
Sbjct: 124 LNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNHD 183

Query: 183 VMTWLSAALTYHDTCTDGFADVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDLPVE 242
           +MTWLSAALTYHDTCT GF DVADL VKD+VEAKLSDLSEMISNSLAIFSGFGGGDLPVE
Sbjct: 184 IMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPVE 243

Query: 243 NRRRRRLMESGTQPWAAENGGD-GGFPQWVSGKDRRLLATPISAIQADIVVAKDGSGKFK 302
           NR+RRRLMES T  WAAENGGD  GFP W+SGKDRRLLA P+S IQADIVVAKDGSGKFK
Sbjct: 244 NRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFK 303

Query: 303 TVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIFD 362
           TVAEAI+AAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKS++D
Sbjct: 304 TVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYD 363

Query: 363 KVTTFRTATFAGSGTGIILRDMTFENTAGASKHQAVALRLSADHAVVYRCNIIGYQDTLY 422
           KVTTFRTATFAGSGT IILRDMTFENTAG SKHQAVALRLSADHAVVY CNIIGYQDTLY
Sbjct: 364 KVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLY 423

Query: 423 VHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGI 482
           VHSNRQFFRECDIYGT+DFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGI
Sbjct: 424 VHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGI 483

Query: 483 SIHACKIIATSDLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD 542
           SIHACKI+AT DLEASKGSFPT+LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD
Sbjct: 484 SIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD 543

Query: 543 TLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
           TLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL
Sbjct: 544 TLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 603

Query: 603 SAN 605
           S N
Sbjct: 604 STN 604

BLAST of CmUC01G000460 vs. NCBI nr
Match: XP_008437596.1 (PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor 61 [Cucumis melo])

HSP 1 Score: 1093.2 bits (2826), Expect = 0.0e+00
Identity = 555/604 (91.89%), Postives = 578/604 (95.70%), Query Frame = 0

Query: 3   NNNYNKLHSSRSLDSTPATSFHLSPNDPPPPPPSSSLRRRSRILIISFLSVLLIAASVVS 62
           NNNY +LH+S S ++TPATSF LS ND  PPPPSSSLRRRSRILIISF+SVLLIAASVVS
Sbjct: 4   NNNYKQLHTSHSANTTPATSFRLSGND--PPPPSSSLRRRSRILIISFMSVLLIAASVVS 63

Query: 63  AAMLVRSHIASSQPNFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGSLSADVHDLVHIS 122
           AA+LVRS IASSQP+FPHNKPSQAISRACSHTLYPSLCLSSLLSFPG+ SADVHDLVHIS
Sbjct: 64  AAILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHIS 123

Query: 123 LNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSLSLFSTEASNHD 182
           LNLTLQHLTKALYSTSQIPI QIS DPLAHSAYEDCMELLNDA+DAFSLSLFST ASNHD
Sbjct: 124 LNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNHD 183

Query: 183 VMTWLSAALTYHDTCTDGFADVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDLPVE 242
           +MTWLSAALTYHDTCTDGF DV DL VK++VEAKLSDLSEMISNSLAIFSGFGGGDLPVE
Sbjct: 184 IMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPVE 243

Query: 243 NRRRRRLMESGT-QPWAAENGGD-GGFPQWVSGKDRRLLATPISAIQADIVVAKDGSGKF 302
           NR+RRRLMESGT   WAAENGGD  GFP+W+SGKDRRLLA PISAIQADIVVAKDGSGKF
Sbjct: 244 NRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGKF 303

Query: 303 KTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIF 362
           KTVAEAI+AAPSSSGRRI+IYIKAGKYEEE LKVGRKKTNLMFVGDGKGITVISGGKS++
Sbjct: 304 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSVY 363

Query: 363 DKVTTFRTATFAGSGTGIILRDMTFENTAGASKHQAVALRLSADHAVVYRCNIIGYQDTL 422
           DKVTTFRTATFAGSGTGIILRDM+FENTAG SKHQAVALRLSADHAVVYRCNIIGYQDTL
Sbjct: 364 DKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTL 423

Query: 423 YVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 482
           YVHSNRQFFRECDIYGT+DFIFGNAVVVFQSCNIYARKPMAGQK TITAQDRKDPNQNTG
Sbjct: 424 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKXTITAQDRKDPNQNTG 483

Query: 483 ISIHACKIIATSDLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 542
           ISIHACKI+AT DLEASKGSFPT+LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 484 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 543

Query: 543 DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 602
           DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG
Sbjct: 544 DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 603

Query: 603 LSAN 605
           LS N
Sbjct: 604 LSTN 605

BLAST of CmUC01G000460 vs. NCBI nr
Match: KAG6579321.1 (putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1039.6 bits (2687), Expect = 1.0e-299
Identity = 536/606 (88.45%), Postives = 563/606 (92.90%), Query Frame = 0

Query: 2   INNNYNKLHSSRSLDSTPATSFHLS--PNDPPPPPPSSSLRRRSRILIISFLSVLLIAAS 61
           +NNNYNKLHSSRS D TPA+SF +S   +DP PPP  SS RR  RIL+ISFLS+ LI AS
Sbjct: 1   MNNNYNKLHSSRSGD-TPASSFRVSNDHSDPLPPPSPSSSRR--RILLISFLSIFLITAS 60

Query: 62  VVSAAMLVRSHI-ASSQPNFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGSLSADVHDL 121
            VSA +LV S I AS++ +FPHNKP+QAISRACS TLYPSLCLSSLLS PGSLSADVHDL
Sbjct: 61  AVSAVVLVHSRIAASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDL 120

Query: 122 VHISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSLSLFSTEA 181
           VHISLNLTLQHLTKALYSTSQIPISQISNDPLA SAYEDCMELLNDAV+AFSLSLFST A
Sbjct: 121 VHISLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTA 180

Query: 182 SNHDVMTWLSAALTYHDTCTDGFADVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGD 241
           +NHDVMTWLSAALTYHDTCTDGF+DVAD  +KDQ+ AKLSDLSEMISNSLAIFSGFGG D
Sbjct: 181 TNHDVMTWLSAALTYHDTCTDGFSDVAD-PIKDQITAKLSDLSEMISNSLAIFSGFGGDD 240

Query: 242 LPVENRRRRRLMESGTQPWAAENGGDGGFPQWVSGKDRRLLATPISAIQADIVVAKDGSG 301
           LPVENRRRRRLMES     A EN GDG FPQWVSGKDRRLLA PIS+IQADIVVAKDGSG
Sbjct: 241 LPVENRRRRRLMESDEHWLAEENNGDGDFPQWVSGKDRRLLAAPISSIQADIVVAKDGSG 300

Query: 302 KFKTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKS 361
           KFKTVAEAI AAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKS
Sbjct: 301 KFKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKS 360

Query: 362 IFDKVTTFRTATFAGSGTGIILRDMTFENTAGASKHQAVALRLSADHAVVYRCNIIGYQD 421
           ++DKVTTFRTATFAGSGTG+ILRDMTFENTAG +KHQAVALRLSADHAVV+R N+IGYQD
Sbjct: 361 VYDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQD 420

Query: 422 TLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQN 481
           TLYVHSNRQFFRECDIYGT+DFIFGNA VVFQSCNIYARKPM+GQKNTITAQDRKDPNQN
Sbjct: 421 TLYVHSNRQFFRECDIYGTIDFIFGNAAVVFQSCNIYARKPMSGQKNTITAQDRKDPNQN 480

Query: 482 TGISIHACKIIATSDLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF 541
           TGISIHACKIIAT DLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF
Sbjct: 481 TGISIHACKIIATGDLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF 540

Query: 542 ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFS 601
           ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITST+EA+KFTVGQFIYGSSWLPSTGVSFS
Sbjct: 541 ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFS 600

Query: 602 AGLSAN 605
           AGLS N
Sbjct: 601 AGLSTN 602

BLAST of CmUC01G000460 vs. NCBI nr
Match: XP_022972980.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita maxima])

HSP 1 Score: 1035.8 bits (2677), Expect = 1.5e-298
Identity = 532/607 (87.64%), Postives = 560/607 (92.26%), Query Frame = 0

Query: 2   INNNYNKLHSSRSLDSTPATSFHLS---PNDPPPPPPSSSLRRRSRILIISFLSVLLIAA 61
           +NNNYNKLHSSRS D TP TSF +S    N PPPPPPSSS R   RIL+ISF S+LLI A
Sbjct: 1   MNNNYNKLHSSRSGD-TPVTSFRVSNDHSNPPPPPPPSSSHR---RILLISFFSILLITA 60

Query: 62  SVVSAAMLVRSHI-ASSQPNFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGSLSADVHD 121
           S VSA +LV S I  S++ +FPHNKP+QAISRACS TLYPSLC+SSLLS PGSLSADVHD
Sbjct: 61  STVSAVVLVHSRITTSTESHFPHNKPTQAISRACSLTLYPSLCISSLLSAPGSLSADVHD 120

Query: 122 LVHISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSLSLFSTE 181
           LVHISLNLTLQHLTKALYSTSQIPISQISNDPLA SAYEDCMELLNDAV+AFSLSLFST 
Sbjct: 121 LVHISLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTT 180

Query: 182 ASNHDVMTWLSAALTYHDTCTDGFADVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGG 241
           A+NHDVMTWLSAALTYHDTCTDGF+DVAD  VKDQ+ AKLSDLSEMISNSLAIFSGFGGG
Sbjct: 181 ATNHDVMTWLSAALTYHDTCTDGFSDVAD-PVKDQITAKLSDLSEMISNSLAIFSGFGGG 240

Query: 242 DLPVENRRRRRLMESGTQPWAAENGGDGGFPQWVSGKDRRLLATPISAIQADIVVAKDGS 301
           DLPVENRRRRRLMES  Q  A EN GDG FP+WVSGKDRRLLA PIS+IQADIVVAKDGS
Sbjct: 241 DLPVENRRRRRLMESDEQWLAEENNGDGDFPRWVSGKDRRLLAAPISSIQADIVVAKDGS 300

Query: 302 GKFKTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 361
           GKFKTVAEAI AAPSSS RRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK
Sbjct: 301 GKFKTVAEAIAAAPSSSSRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 360

Query: 362 SIFDKVTTFRTATFAGSGTGIILRDMTFENTAGASKHQAVALRLSADHAVVYRCNIIGYQ 421
           S++DKVTTFRTATFAGSGTG+ILRDMTFENTAG +KHQAVALRLSADH VV+R N+IGYQ
Sbjct: 361 SVYDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHTVVFRSNVIGYQ 420

Query: 422 DTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQ 481
           DTLYVHSNRQFFRECDIYGT+DFIFGNA VVFQSCNIYARKPM+GQKNTITAQDRKDPNQ
Sbjct: 421 DTLYVHSNRQFFRECDIYGTIDFIFGNAAVVFQSCNIYARKPMSGQKNTITAQDRKDPNQ 480

Query: 482 NTGISIHACKIIATSDLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGS 541
           NTGISIHACKIIAT DL+ASKGSFPTYLGRPWKLYSRVVYMVSS+GDHIHPRGWLEWQGS
Sbjct: 481 NTGISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSLGDHIHPRGWLEWQGS 540

Query: 542 FALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSF 601
           FALDTLYYGEYMNSGP AAVGQRVKWPGYRVITS +EA+KFTVGQFIYGSSWLPSTGVSF
Sbjct: 541 FALDTLYYGEYMNSGPSAAVGQRVKWPGYRVITSAIEATKFTVGQFIYGSSWLPSTGVSF 600

Query: 602 SAGLSAN 605
           SAGLS N
Sbjct: 601 SAGLSTN 602

BLAST of CmUC01G000460 vs. ExPASy Swiss-Prot
Match: Q9FK05 (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 4.2e-203
Identity = 371/587 (63.20%), Postives = 440/587 (74.96%), Query Frame = 0

Query: 26  SPNDPPPPPPSSSLRRRSRILIISF-LSVLLIAASVVSAAMLVRSHIASSQPNFPHNKPS 85
           +PN   P      L+++++  +I F L+VL++          +R+  +         KP+
Sbjct: 13  NPNQKDPATSLPELQKKTKTKLILFTLAVLVVGVVCFGIFAGIRAVDSGKTEPKLTRKPT 72

Query: 86  QAISRACSHTLYPSLCLSSLLSFPGSLSADVHDLVHISLNLTLQHLTKALYSTSQIPISQ 145
           QAISR CS +LYP+LC+ +LL FPGSL+AD ++L+HIS N TLQ  +KALY++S I  +Q
Sbjct: 73  QAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSSTITYTQ 132

Query: 146 ISNDPLAHSAYEDCMELLNDAVDAFSLSLFST-----EASNHDVMTWLSAALTYHDTCTD 205
           +   P   SAY+ C+ELL+D+VDA + +L S      + S+ DVMTWLS+A+T HDTCTD
Sbjct: 133 M--PPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTNHDTCTD 192

Query: 206 GFADVADLA--VKDQVEAKLSDLSEMISNSLAIFSG--FGGGDLPVENRRRRRLMESGTQ 265
           GF ++      VKDQV   + DLSEM+SN LAIF+G       +PV N R+    E   +
Sbjct: 193 GFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETEE 252

Query: 266 PWAAENGGDGGFPQWVSGKDRRLLATPISAIQADIVVAKDGSGKFKTVAEAIDAAPSSSG 325
                       P W+  +DR LL TP SAIQADI V+KDGSG FKT+AEAI  AP  S 
Sbjct: 253 -----------LPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSS 312

Query: 326 RRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIFDKVTTFRTATFAGSG 385
           RR VIY+KAG+YEEENLKVGRKKTNLMF+GDGKG TVI+GGKSI D +TTF TATFA +G
Sbjct: 313 RRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATG 372

Query: 386 TGIILRDMTFENTAGASKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIY 445
            G I+RDMTFEN AG +KHQAVALR+  DHAVVYRCNIIGYQD LYVHSNRQFFREC+IY
Sbjct: 373 AGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIY 432

Query: 446 GTVDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIIATSDLE 505
           GTVDFIFGNA V+ QSCNIYARKPMA QK TITAQ+RKDPNQNTGISIHACK++AT DLE
Sbjct: 433 GTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLE 492

Query: 506 ASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGA 565
           ASKGS+PTYLGRPWKLYSRVVYM+S MGDHI PRGWLEW G FALD+LYYGEYMN G G+
Sbjct: 493 ASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGS 552

Query: 566 AVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 603
            +GQRVKWPGY VITSTVEASKFTV QFI GSSWLPSTGVSF +GLS
Sbjct: 553 GIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

BLAST of CmUC01G000460 vs. ExPASy Swiss-Prot
Match: Q9M3B0 (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3702 GN=PME34 PE=2 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 1.5e-197
Identity = 355/578 (61.42%), Postives = 437/578 (75.61%), Query Frame = 0

Query: 35  PSSSLRRRSRILIIS--FLSVLLIAASVVSAAMLVRSHIASSQPNFPHNKPSQAISRACS 94
           P ++ RR  + L++S   L++ LI A+ + A +  R  +  S P     KPSQAIS+AC 
Sbjct: 34  PENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLNQSVPGLA-RKPSQAISKACE 93

Query: 95  HTLYPSLCLSSLLSFPGSLSA-DVHDLVHISLNLTLQHLTKALYSTSQIPISQISNDPLA 154
            T +P LC+ SL+ FPGSL+A    DL+H+++N+TL H + ALYS++   +S +   P A
Sbjct: 94  LTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSAS--LSFVDMPPRA 153

Query: 155 HSAYEDCMELLNDAVDAFS---LSLFSTEASNHDVMTWLSAALTYHDTCTDGFADVADLA 214
            SAY+ C+ELL+D+VDA S    S+ S+ A   DV TWLSAALT HDTCT+GF  V D  
Sbjct: 154 RSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLSAALTNHDTCTEGFDGVDDGG 213

Query: 215 VKDQVEAKLSDLSEMISNSLAIFSGFGGGD----LPVENRRRRRLMESGTQPWAAENGGD 274
           VKD + A L +LSE++SN LAIFS    GD    +P++NRR   + E            +
Sbjct: 214 VKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLGVEER-----------E 273

Query: 275 GGFPQWVSGKDRRLLATPISAIQADIVVAKDGSGKFKTVAEAIDAAPSSSGRRIVIYIKA 334
             FP+W+  K+R +L  P+S IQADI+V+KDG+G  KT++EAI  AP +S RRI+IY+KA
Sbjct: 274 EKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKA 333

Query: 335 GKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIFDKVTTFRTATFAGSGTGIILRDMT 394
           G+YEE NLKVGRKK NLMFVGDGKG TVISGGKSIFD +TTF TA+FA +G G I RD+T
Sbjct: 334 GRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFIARDIT 393

Query: 395 FENTAGASKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGN 454
           FEN AG +KHQAVALR+ ADHAV+YRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGN
Sbjct: 394 FENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGN 453

Query: 455 AVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIIATSDLEASKGSFPTY 514
           A VV Q+C+IYARKPM  QKNTITAQ+RKDPNQNTGISIHA +++A SDL+A+ GS  TY
Sbjct: 454 AAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQATNGSTQTY 513

Query: 515 LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRVKWP 574
           LGRPWKL+SR VYM+S +G H+H RGWLEW  +FALDTLYYGEY+NSGPG+ +GQRV WP
Sbjct: 514 LGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWP 573

Query: 575 GYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 603
           GYRVI ST EA++FTV +FIYGSSWLPSTGVSF AGLS
Sbjct: 574 GYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597

BLAST of CmUC01G000460 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 458.8 bits (1179), Expect = 9.8e-128
Identity = 259/579 (44.73%), Postives = 344/579 (59.41%), Query Frame = 0

Query: 37  SSLRRRSRILIISFLSVLLIAASVVSAAMLVRSHIASSQPNFPHNKPSQAISRACSHTLY 96
           S  +   ++++ S    LL+ AS+V  A    +   + +     +     +   CS TLY
Sbjct: 12  SDFKNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLY 71

Query: 97  PSLCLSSLLSFPGSLSADVHDLVHISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYE 156
           P LC S++ +  G       +++  SLNLT + +    ++  ++   +    P   +A  
Sbjct: 72  PELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALH 131

Query: 157 DCMELLNDAVDAFSLSL---------FSTEASNHDVMTWLSAALTYHDTCTDGFA-DVAD 216
           DC+E +++ +D   +++          S      D+ T +S+A+T   TC DGF+ D AD
Sbjct: 132 DCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDAD 191

Query: 217 LAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDLP-VENRRRRRLMESGTQPWAAENGGD- 276
             V+  +      +  M SN+LA+       D+   E R +     +       E  GD 
Sbjct: 192 RKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDL 251

Query: 277 --GGFPQWVSGKDRRLLATPISAIQADIVVAKDGSGKFKTVAEAIDAAPSSSGRRIVIYI 336
              G+P+W+S  DRRLL    S I+AD  VA DGSG F TVA A+ AAP  S +R VI+I
Sbjct: 252 DSDGWPKWLSVGDRRLLQG--STIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIHI 311

Query: 337 KAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIFDKVTTFRTATFAGSGTGIILRD 396
           KAG Y  EN++V +KKTN+MF+GDG+G T+I+G +++ D  TTF +AT A  G   + RD
Sbjct: 312 KAGVY-RENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLARD 371

Query: 397 MTFENTAGASKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIF 456
           +TF+NTAG SKHQAVALR+ +D +  Y+C++  YQDTLYVHSNRQFF +C I GTVDFIF
Sbjct: 372 ITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFIF 431

Query: 457 GNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIIATSDLEASKGSFP 516
           GNA  V Q C+I AR+P +GQKN +TAQ R DPNQNTGI I  C+I  TSDL A KG+FP
Sbjct: 432 GNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFP 491

Query: 517 TYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRVK 576
           TYLGRPWK YSR V M S + D I P GW EW GSFALDTL Y EY+N G GA    RVK
Sbjct: 492 TYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRVK 551

Query: 577 WPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
           W GY+VITS  EA  FT GQFI G  WL STG  FS  L
Sbjct: 552 WKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of CmUC01G000460 vs. ExPASy Swiss-Prot
Match: Q43143 (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 9.8e-128
Identity = 260/584 (44.52%), Postives = 365/584 (62.50%), Query Frame = 0

Query: 40  RRRSRILIISFLSVLLIAASVVSAAMLVRSHIASSQPNFP----HNKPSQAISRACSHTL 99
           +R+ +I +    SVLL+AA V+     V+SH  +S  +       +     +  ACS+TL
Sbjct: 16  KRKKKIYLAIVASVLLVAA-VIGVVAGVKSHSKNSDDHADIMAISSSAHAIVKSACSNTL 75

Query: 100 YPSLCLSSLLS---FPGSLSADVHDLVHISLNLTLQHLTKALYSTSQIPISQISNDPLAH 159
           +P LC S++++   F   +++   D++ +SLN+T++ + +  Y+  ++  ++    P   
Sbjct: 76  HPELCYSAIVNVSDFSKKVTSQ-KDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREK 135

Query: 160 SAYEDCMELLNDAVDAF-----SLSLFSTEAS----NHDVMTWLSAALTYHDTCTDGFA- 219
            A  DC+E +++ +D        L L+  + S      D+ T +S+A+T  +TC DGF+ 
Sbjct: 136 VALHDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSAITNQETCLDGFSH 195

Query: 220 DVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDLPVENR-----RRRRLMESGTQPW 279
           D AD  V+  +      + +M SN+LA+       D+  E +       R+L+E      
Sbjct: 196 DEADKKVRKVLLKGQKHVEKMCSNALAMICNMTDTDIANEMKLSAPANNRKLVED----- 255

Query: 280 AAENGGDGGFPQWVSGKDRRLLATPISAIQADIVVAKDGSGKFKTVAEAIDAAPSSSGRR 339
                 +G +P+W+S  DRRLL +  S +  D+VVA DGSG +KTV+EA+  AP  S +R
Sbjct: 256 ------NGEWPEWLSAGDRRLLQS--STVTPDVVVAADGSGDYKTVSEAVRKAPEKSSKR 315

Query: 340 IVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIFDKVTTFRTATFAGSGTG 399
            VI IKAG Y  EN+ V +KKTN+MF+GDGK  T+I+  +++ D  TTF +AT       
Sbjct: 316 YVIRIKAGVY-RENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGSTTFHSATVVRVAGK 375

Query: 400 IILRDMTFENTAGASKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGT 459
           ++ RD+TF+NTAGASKHQAVAL + +D +  YRC+++ YQDTLYVHSNRQFF +C + GT
Sbjct: 376 VLARDITFQNTAGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQCLVAGT 435

Query: 460 VDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIIATSDLEAS 519
           VDFIFGN   VFQ C+I+AR+P +GQKN +TAQ R DPNQNTGI I  C+I ATSDL   
Sbjct: 436 VDFIFGNGAAVFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPV 495

Query: 520 KGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAV 579
           + SFPTYLGRPWK YSR V M SS+ D I P GW EW G+FALDTL+YGEY N+G GA  
Sbjct: 496 QKSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFYGEYANTGAGAPT 555

Query: 580 GQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
             RVKW G++VITS+ EA  +T G+FI G SWL STG  FS GL
Sbjct: 556 SGRVKWKGHKVITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583

BLAST of CmUC01G000460 vs. ExPASy Swiss-Prot
Match: O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 457.2 bits (1175), Expect = 2.8e-127
Identity = 260/589 (44.14%), Postives = 364/589 (61.80%), Query Frame = 0

Query: 38  SLRRRSRILIISFLSVLLIAASV--VSAAMLVRSHIASSQPNFPHNKPSQAISR-ACSHT 97
           + ++  +++++S    LL  A+V  +SA     +   +  P+      S A+ R +CS T
Sbjct: 13  NFKKNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPS------SHAVLRSSCSST 72

Query: 98  LYPSLCLSSLLSFPGSLSADVHDLVHISLNLTLQHLTKALYSTSQIPISQISNDPLAHSA 157
            YP LC+S++++  G       D++  S+NLT+  +    ++  ++   +    P   +A
Sbjct: 73  RYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTA 132

Query: 158 YEDCMELLNDAVDAF-----SLSLFSTEAS--NH--DVMTWLSAALTYHDTCTDGFA-DV 217
             DC+E +++ +D        L L+ T+ +   H  D+ T +S+A+T  +TC DGF+ D 
Sbjct: 133 LHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDD 192

Query: 218 ADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDL-------PVENRRRRRLMESGTQPW 277
           AD  V+  +      +  M SN+LA+       D+        + +  R+   E+     
Sbjct: 193 ADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTV 252

Query: 278 AAENGGDG-----GFPQWVSGKDRRLLATPISAIQADIVVAKDGSGKFKTVAEAIDAAPS 337
           A +  G G     G+P W+S  DRRLL    S ++AD  VA DGSG FKTVA A+ AAP 
Sbjct: 253 AVDIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAAPE 312

Query: 338 SSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIFDKVTTFRTATFA 397
           +S +R VI+IKAG Y  EN++V +KK N+MF+GDG+  T+I+G +++ D  TTF +AT A
Sbjct: 313 NSNKRYVIHIKAGVY-RENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVA 372

Query: 398 GSGTGIILRDMTFENTAGASKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFREC 457
             G   + RD+TF+NTAG SKHQAVALR+ +D +  Y C+++ YQDTLYVHSNRQFF +C
Sbjct: 373 AVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKC 432

Query: 458 DIYGTVDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIIATS 517
            I GTVDFIFGNA VV Q C+I+AR+P +GQKN +TAQ R DPNQNTGI I  C+I ATS
Sbjct: 433 LIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATS 492

Query: 518 DLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSG 577
           DL++ KGSFPTYLGRPWK YS+ V M S++ D I P GW EW G+FAL+TL Y EY N+G
Sbjct: 493 DLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTG 552

Query: 578 PGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
            GA    RVKW G++VIT+  EA K+T GQFI G  WL STG  FS GL
Sbjct: 553 AGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of CmUC01G000460 vs. ExPASy TrEMBL
Match: A0A0A0KJG1 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G139430 PE=3 SV=1)

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 551/603 (91.38%), Postives = 575/603 (95.36%), Query Frame = 0

Query: 3   NNNYNKLHSSRSLDSTPATSFHLSPNDPPPPPPSSSLRRRSRILIISFLSVLLIAASVVS 62
           NN+YNKLH+S S ++TPA SF LS ND  PPPPSSSLRRRSRILIISF+SVLLIAASVVS
Sbjct: 4   NNSYNKLHTSHSANTTPAASFRLSGND--PPPPSSSLRRRSRILIISFMSVLLIAASVVS 63

Query: 63  AAMLVRSHIASSQPNFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGSLSADVHDLVHIS 122
           AA+LVRSH+ SSQP+FPHNKPSQAISRACSHTLYPSLCLSSLLSFPG+ SADVHDLVHIS
Sbjct: 64  AAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHIS 123

Query: 123 LNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSLSLFSTEASNHD 182
           LNLTLQHLTKALYSTSQIP+ QIS DPLAHSAYEDCMELLNDA+DAFSLSLFS +ASNHD
Sbjct: 124 LNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNHD 183

Query: 183 VMTWLSAALTYHDTCTDGFADVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDLPVE 242
           +MTWLSAALTYHDTCT GF DVADL VKD+VEAKLSDLSEMISNSLAIFSGFGGGDLPVE
Sbjct: 184 IMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPVE 243

Query: 243 NRRRRRLMESGTQPWAAENGGD-GGFPQWVSGKDRRLLATPISAIQADIVVAKDGSGKFK 302
           NR+RRRLMES T  WAAENGGD  GFP W+SGKDRRLLA P+S IQADIVVAKDGSGKFK
Sbjct: 244 NRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFK 303

Query: 303 TVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIFD 362
           TVAEAI+AAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKS++D
Sbjct: 304 TVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYD 363

Query: 363 KVTTFRTATFAGSGTGIILRDMTFENTAGASKHQAVALRLSADHAVVYRCNIIGYQDTLY 422
           KVTTFRTATFAGSGT IILRDMTFENTAG SKHQAVALRLSADHAVVY CNIIGYQDTLY
Sbjct: 364 KVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLY 423

Query: 423 VHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGI 482
           VHSNRQFFRECDIYGT+DFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGI
Sbjct: 424 VHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGI 483

Query: 483 SIHACKIIATSDLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD 542
           SIHACKI+AT DLEASKGSFPT+LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD
Sbjct: 484 SIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD 543

Query: 543 TLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
           TLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL
Sbjct: 544 TLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 603

Query: 603 SAN 605
           S N
Sbjct: 604 STN 604

BLAST of CmUC01G000460 vs. ExPASy TrEMBL
Match: A0A1S3AUZ6 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482964 PE=3 SV=1)

HSP 1 Score: 1093.2 bits (2826), Expect = 0.0e+00
Identity = 555/604 (91.89%), Postives = 578/604 (95.70%), Query Frame = 0

Query: 3   NNNYNKLHSSRSLDSTPATSFHLSPNDPPPPPPSSSLRRRSRILIISFLSVLLIAASVVS 62
           NNNY +LH+S S ++TPATSF LS ND  PPPPSSSLRRRSRILIISF+SVLLIAASVVS
Sbjct: 4   NNNYKQLHTSHSANTTPATSFRLSGND--PPPPSSSLRRRSRILIISFMSVLLIAASVVS 63

Query: 63  AAMLVRSHIASSQPNFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGSLSADVHDLVHIS 122
           AA+LVRS IASSQP+FPHNKPSQAISRACSHTLYPSLCLSSLLSFPG+ SADVHDLVHIS
Sbjct: 64  AAILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHIS 123

Query: 123 LNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSLSLFSTEASNHD 182
           LNLTLQHLTKALYSTSQIPI QIS DPLAHSAYEDCMELLNDA+DAFSLSLFST ASNHD
Sbjct: 124 LNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNHD 183

Query: 183 VMTWLSAALTYHDTCTDGFADVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDLPVE 242
           +MTWLSAALTYHDTCTDGF DV DL VK++VEAKLSDLSEMISNSLAIFSGFGGGDLPVE
Sbjct: 184 IMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPVE 243

Query: 243 NRRRRRLMESGT-QPWAAENGGD-GGFPQWVSGKDRRLLATPISAIQADIVVAKDGSGKF 302
           NR+RRRLMESGT   WAAENGGD  GFP+W+SGKDRRLLA PISAIQADIVVAKDGSGKF
Sbjct: 244 NRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGKF 303

Query: 303 KTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIF 362
           KTVAEAI+AAPSSSGRRI+IYIKAGKYEEE LKVGRKKTNLMFVGDGKGITVISGGKS++
Sbjct: 304 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSVY 363

Query: 363 DKVTTFRTATFAGSGTGIILRDMTFENTAGASKHQAVALRLSADHAVVYRCNIIGYQDTL 422
           DKVTTFRTATFAGSGTGIILRDM+FENTAG SKHQAVALRLSADHAVVYRCNIIGYQDTL
Sbjct: 364 DKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTL 423

Query: 423 YVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 482
           YVHSNRQFFRECDIYGT+DFIFGNAVVVFQSCNIYARKPMAGQK TITAQDRKDPNQNTG
Sbjct: 424 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKXTITAQDRKDPNQNTG 483

Query: 483 ISIHACKIIATSDLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 542
           ISIHACKI+AT DLEASKGSFPT+LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 484 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 543

Query: 543 DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 602
           DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG
Sbjct: 544 DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 603

Query: 603 LSAN 605
           LS N
Sbjct: 604 LSTN 605

BLAST of CmUC01G000460 vs. ExPASy TrEMBL
Match: A0A6J1IA52 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111471502 PE=3 SV=1)

HSP 1 Score: 1035.8 bits (2677), Expect = 7.2e-299
Identity = 532/607 (87.64%), Postives = 560/607 (92.26%), Query Frame = 0

Query: 2   INNNYNKLHSSRSLDSTPATSFHLS---PNDPPPPPPSSSLRRRSRILIISFLSVLLIAA 61
           +NNNYNKLHSSRS D TP TSF +S    N PPPPPPSSS R   RIL+ISF S+LLI A
Sbjct: 1   MNNNYNKLHSSRSGD-TPVTSFRVSNDHSNPPPPPPPSSSHR---RILLISFFSILLITA 60

Query: 62  SVVSAAMLVRSHI-ASSQPNFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGSLSADVHD 121
           S VSA +LV S I  S++ +FPHNKP+QAISRACS TLYPSLC+SSLLS PGSLSADVHD
Sbjct: 61  STVSAVVLVHSRITTSTESHFPHNKPTQAISRACSLTLYPSLCISSLLSAPGSLSADVHD 120

Query: 122 LVHISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSLSLFSTE 181
           LVHISLNLTLQHLTKALYSTSQIPISQISNDPLA SAYEDCMELLNDAV+AFSLSLFST 
Sbjct: 121 LVHISLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTT 180

Query: 182 ASNHDVMTWLSAALTYHDTCTDGFADVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGG 241
           A+NHDVMTWLSAALTYHDTCTDGF+DVAD  VKDQ+ AKLSDLSEMISNSLAIFSGFGGG
Sbjct: 181 ATNHDVMTWLSAALTYHDTCTDGFSDVAD-PVKDQITAKLSDLSEMISNSLAIFSGFGGG 240

Query: 242 DLPVENRRRRRLMESGTQPWAAENGGDGGFPQWVSGKDRRLLATPISAIQADIVVAKDGS 301
           DLPVENRRRRRLMES  Q  A EN GDG FP+WVSGKDRRLLA PIS+IQADIVVAKDGS
Sbjct: 241 DLPVENRRRRRLMESDEQWLAEENNGDGDFPRWVSGKDRRLLAAPISSIQADIVVAKDGS 300

Query: 302 GKFKTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 361
           GKFKTVAEAI AAPSSS RRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK
Sbjct: 301 GKFKTVAEAIAAAPSSSSRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 360

Query: 362 SIFDKVTTFRTATFAGSGTGIILRDMTFENTAGASKHQAVALRLSADHAVVYRCNIIGYQ 421
           S++DKVTTFRTATFAGSGTG+ILRDMTFENTAG +KHQAVALRLSADH VV+R N+IGYQ
Sbjct: 361 SVYDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHTVVFRSNVIGYQ 420

Query: 422 DTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQ 481
           DTLYVHSNRQFFRECDIYGT+DFIFGNA VVFQSCNIYARKPM+GQKNTITAQDRKDPNQ
Sbjct: 421 DTLYVHSNRQFFRECDIYGTIDFIFGNAAVVFQSCNIYARKPMSGQKNTITAQDRKDPNQ 480

Query: 482 NTGISIHACKIIATSDLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGS 541
           NTGISIHACKIIAT DL+ASKGSFPTYLGRPWKLYSRVVYMVSS+GDHIHPRGWLEWQGS
Sbjct: 481 NTGISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSLGDHIHPRGWLEWQGS 540

Query: 542 FALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSF 601
           FALDTLYYGEYMNSGP AAVGQRVKWPGYRVITS +EA+KFTVGQFIYGSSWLPSTGVSF
Sbjct: 541 FALDTLYYGEYMNSGPSAAVGQRVKWPGYRVITSAIEATKFTVGQFIYGSSWLPSTGVSF 600

Query: 602 SAGLSAN 605
           SAGLS N
Sbjct: 601 SAGLSTN 602

BLAST of CmUC01G000460 vs. ExPASy TrEMBL
Match: A0A6J1E2V1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430323 PE=3 SV=1)

HSP 1 Score: 1034.2 bits (2673), Expect = 2.1e-298
Identity = 533/606 (87.95%), Postives = 562/606 (92.74%), Query Frame = 0

Query: 2   INNNYNKLHSSRSLDSTPATSFHLS--PNDPPPPPPSSSLRRRSRILIISFLSVLLIAAS 61
           +NNNYN LHSS S D TPA+SF +S   +DP PPP  SS RR  RIL+ISF S+LLI AS
Sbjct: 1   MNNNYNMLHSSHSGD-TPASSFRVSNDHSDPLPPPSPSSSRR--RILLISFFSILLITAS 60

Query: 62  VVSAAMLVRSHI-ASSQPNFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGSLSADVHDL 121
            VSA +LV S I AS++ +FPHNKP+QAISRACS TLYPSLCLSSLLS PGSLSADVHDL
Sbjct: 61  AVSAVVLVHSRIAASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDL 120

Query: 122 VHISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSLSLFSTEA 181
           VHISLNLTLQHLTKALYSTSQIPISQISNDPLA SAYEDCMELLNDAV+AFSLSLFST A
Sbjct: 121 VHISLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTA 180

Query: 182 SNHDVMTWLSAALTYHDTCTDGFADVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGD 241
           +NHDVMTWLSAALTYHDTCTDGF+DVAD  +KDQ+ AKLSDLSEMISNSLAIFSGFGG D
Sbjct: 181 TNHDVMTWLSAALTYHDTCTDGFSDVAD-PIKDQITAKLSDLSEMISNSLAIFSGFGGDD 240

Query: 242 LPVENRRRRRLMESGTQPWAAENGGDGGFPQWVSGKDRRLLATPISAIQADIVVAKDGSG 301
           LPVENRRRRRLMES  Q  A EN GDG FP+WVSGKDRRLLA PIS+IQADIVVAKDGSG
Sbjct: 241 LPVENRRRRRLMESDEQWLAEENNGDGDFPRWVSGKDRRLLAAPISSIQADIVVAKDGSG 300

Query: 302 KFKTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKS 361
           KFKTVAEAI AAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKS
Sbjct: 301 KFKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKS 360

Query: 362 IFDKVTTFRTATFAGSGTGIILRDMTFENTAGASKHQAVALRLSADHAVVYRCNIIGYQD 421
           ++DKVTTFRTATFAGSGTG+ILRDMTFENTAG +KHQAVALRLSADHAVV+R N+IGYQD
Sbjct: 361 VYDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQD 420

Query: 422 TLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQN 481
           TLYVHSNRQFFRECDIYGT+DFIFGNAVVVFQSCNIYARKPM+GQKNTITAQDRKDPNQN
Sbjct: 421 TLYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMSGQKNTITAQDRKDPNQN 480

Query: 482 TGISIHACKIIATSDLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF 541
           TGISIHACKIIAT DL+ASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF
Sbjct: 481 TGISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF 540

Query: 542 ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFS 601
           ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITS +EA+KFTVGQFIYGSSWLPSTGVSFS
Sbjct: 541 ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSAIEATKFTVGQFIYGSSWLPSTGVSFS 600

Query: 602 AGLSAN 605
           AGLS N
Sbjct: 601 AGLSTN 602

BLAST of CmUC01G000460 vs. ExPASy TrEMBL
Match: A0A6J1H2K1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459120 PE=3 SV=1)

HSP 1 Score: 995.7 bits (2573), Expect = 8.2e-287
Identity = 519/604 (85.93%), Postives = 552/604 (91.39%), Query Frame = 0

Query: 4   NNYNKLHSSRSLDSTPATSFHLSPNDPPPPPPSSSLRRRSRILIISFLSVLLIAASVVSA 63
           NNYNKLHSS S D TPAT F +S    P PP SS+ RRR+RIL +S +S+LLI AS VSA
Sbjct: 2   NNYNKLHSSASGD-TPAT-FQVS--HTPNPPSSSASRRRTRILFVSLISILLITASAVSA 61

Query: 64  AMLVRSHIASSQPNFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGSLSADVHDLVHISL 123
           A+L+     S   +FPHNKP+QAIS ACSHTLYPSLC SSLLSFPGSLSADVH LVHISL
Sbjct: 62  AVLLH----SQSSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISL 121

Query: 124 NLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSLSLFSTEASNHDV 183
           NLTLQHLTKALY++SQIP+ QISN+PLAHSAY+DC+ELLNDAVDAFSLSLFST ASNHD+
Sbjct: 122 NLTLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDI 181

Query: 184 MTWLSAALTYHDTCTDGFADVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDLPVEN 243
           MTWLSAALTYHDTC+DGFADV D  VKDQ+ A+LSDLSEMISNSLAIFSG+G G LPV+N
Sbjct: 182 MTWLSAALTYHDTCSDGFADVDD-PVKDQMAARLSDLSEMISNSLAIFSGYGEG-LPVQN 241

Query: 244 RRRRRLMESGTQPWAAENG---GDGGFPQWVSGKDRRLLATPISAIQADIVVAKDGSGKF 303
             RRRLMES  + WAAENG    +G FP WVSGKDRRLLA PISAIQADIVVAKDGSGKF
Sbjct: 242 --RRRLMESDRE-WAAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKF 301

Query: 304 KTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIF 363
           KTVAEAI+AAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKS+F
Sbjct: 302 KTVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVF 361

Query: 364 DKVTTFRTATFAGSGTGIILRDMTFENTAGASKHQAVALRLSADHAVVYRCNIIGYQDTL 423
           DKVTTFRTATFAGSGTGIILRDMTFENTAG SKHQAVALR+SADHAVVYRCNIIGYQDTL
Sbjct: 362 DKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTL 421

Query: 424 YVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 483
           YVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPM  QKNTITAQ+RKDPNQNTG
Sbjct: 422 YVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTG 481

Query: 484 ISIHACKIIATSDLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 543
           ISIHACKI+AT+DLEASKGSFPT+LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 482 ISIHACKILATTDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 541

Query: 544 DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 603
           DTLYYGEYMNSGPGAA GQRV WPGYRVITST+EA+KFTVGQFIYGSSWLPSTGVSFSAG
Sbjct: 542 DTLYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSAG 592

Query: 604 LSAN 605
           LSAN
Sbjct: 602 LSAN 592

BLAST of CmUC01G000460 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 709.1 bits (1829), Expect = 3.0e-204
Identity = 371/587 (63.20%), Postives = 440/587 (74.96%), Query Frame = 0

Query: 26  SPNDPPPPPPSSSLRRRSRILIISF-LSVLLIAASVVSAAMLVRSHIASSQPNFPHNKPS 85
           +PN   P      L+++++  +I F L+VL++          +R+  +         KP+
Sbjct: 13  NPNQKDPATSLPELQKKTKTKLILFTLAVLVVGVVCFGIFAGIRAVDSGKTEPKLTRKPT 72

Query: 86  QAISRACSHTLYPSLCLSSLLSFPGSLSADVHDLVHISLNLTLQHLTKALYSTSQIPISQ 145
           QAISR CS +LYP+LC+ +LL FPGSL+AD ++L+HIS N TLQ  +KALY++S I  +Q
Sbjct: 73  QAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSSTITYTQ 132

Query: 146 ISNDPLAHSAYEDCMELLNDAVDAFSLSLFST-----EASNHDVMTWLSAALTYHDTCTD 205
           +   P   SAY+ C+ELL+D+VDA + +L S      + S+ DVMTWLS+A+T HDTCTD
Sbjct: 133 M--PPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTNHDTCTD 192

Query: 206 GFADVADLA--VKDQVEAKLSDLSEMISNSLAIFSG--FGGGDLPVENRRRRRLMESGTQ 265
           GF ++      VKDQV   + DLSEM+SN LAIF+G       +PV N R+    E   +
Sbjct: 193 GFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETEE 252

Query: 266 PWAAENGGDGGFPQWVSGKDRRLLATPISAIQADIVVAKDGSGKFKTVAEAIDAAPSSSG 325
                       P W+  +DR LL TP SAIQADI V+KDGSG FKT+AEAI  AP  S 
Sbjct: 253 -----------LPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSS 312

Query: 326 RRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIFDKVTTFRTATFAGSG 385
           RR VIY+KAG+YEEENLKVGRKKTNLMF+GDGKG TVI+GGKSI D +TTF TATFA +G
Sbjct: 313 RRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATG 372

Query: 386 TGIILRDMTFENTAGASKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIY 445
            G I+RDMTFEN AG +KHQAVALR+  DHAVVYRCNIIGYQD LYVHSNRQFFREC+IY
Sbjct: 373 AGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIY 432

Query: 446 GTVDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIIATSDLE 505
           GTVDFIFGNA V+ QSCNIYARKPMA QK TITAQ+RKDPNQNTGISIHACK++AT DLE
Sbjct: 433 GTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLE 492

Query: 506 ASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGA 565
           ASKGS+PTYLGRPWKLYSRVVYM+S MGDHI PRGWLEW G FALD+LYYGEYMN G G+
Sbjct: 493 ASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGS 552

Query: 566 AVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 603
            +GQRVKWPGY VITSTVEASKFTV QFI GSSWLPSTGVSF +GLS
Sbjct: 553 GIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

BLAST of CmUC01G000460 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 690.6 bits (1781), Expect = 1.1e-198
Identity = 355/578 (61.42%), Postives = 437/578 (75.61%), Query Frame = 0

Query: 35  PSSSLRRRSRILIIS--FLSVLLIAASVVSAAMLVRSHIASSQPNFPHNKPSQAISRACS 94
           P ++ RR  + L++S   L++ LI A+ + A +  R  +  S P     KPSQAIS+AC 
Sbjct: 34  PENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLNQSVPGLA-RKPSQAISKACE 93

Query: 95  HTLYPSLCLSSLLSFPGSLSA-DVHDLVHISLNLTLQHLTKALYSTSQIPISQISNDPLA 154
            T +P LC+ SL+ FPGSL+A    DL+H+++N+TL H + ALYS++   +S +   P A
Sbjct: 94  LTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSAS--LSFVDMPPRA 153

Query: 155 HSAYEDCMELLNDAVDAFS---LSLFSTEASNHDVMTWLSAALTYHDTCTDGFADVADLA 214
            SAY+ C+ELL+D+VDA S    S+ S+ A   DV TWLSAALT HDTCT+GF  V D  
Sbjct: 154 RSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLSAALTNHDTCTEGFDGVDDGG 213

Query: 215 VKDQVEAKLSDLSEMISNSLAIFSGFGGGD----LPVENRRRRRLMESGTQPWAAENGGD 274
           VKD + A L +LSE++SN LAIFS    GD    +P++NRR   + E            +
Sbjct: 214 VKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLGVEER-----------E 273

Query: 275 GGFPQWVSGKDRRLLATPISAIQADIVVAKDGSGKFKTVAEAIDAAPSSSGRRIVIYIKA 334
             FP+W+  K+R +L  P+S IQADI+V+KDG+G  KT++EAI  AP +S RRI+IY+KA
Sbjct: 274 EKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKA 333

Query: 335 GKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIFDKVTTFRTATFAGSGTGIILRDMT 394
           G+YEE NLKVGRKK NLMFVGDGKG TVISGGKSIFD +TTF TA+FA +G G I RD+T
Sbjct: 334 GRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFIARDIT 393

Query: 395 FENTAGASKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGN 454
           FEN AG +KHQAVALR+ ADHAV+YRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGN
Sbjct: 394 FENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGN 453

Query: 455 AVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIIATSDLEASKGSFPTY 514
           A VV Q+C+IYARKPM  QKNTITAQ+RKDPNQNTGISIHA +++A SDL+A+ GS  TY
Sbjct: 454 AAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQATNGSTQTY 513

Query: 515 LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRVKWP 574
           LGRPWKL+SR VYM+S +G H+H RGWLEW  +FALDTLYYGEY+NSGPG+ +GQRV WP
Sbjct: 514 LGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWP 573

Query: 575 GYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 603
           GYRVI ST EA++FTV +FIYGSSWLPSTGVSF AGLS
Sbjct: 574 GYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597

BLAST of CmUC01G000460 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 458.8 bits (1179), Expect = 6.9e-129
Identity = 259/579 (44.73%), Postives = 344/579 (59.41%), Query Frame = 0

Query: 37  SSLRRRSRILIISFLSVLLIAASVVSAAMLVRSHIASSQPNFPHNKPSQAISRACSHTLY 96
           S  +   ++++ S    LL+ AS+V  A    +   + +     +     +   CS TLY
Sbjct: 12  SDFKNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLY 71

Query: 97  PSLCLSSLLSFPGSLSADVHDLVHISLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYE 156
           P LC S++ +  G       +++  SLNLT + +    ++  ++   +    P   +A  
Sbjct: 72  PELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALH 131

Query: 157 DCMELLNDAVDAFSLSL---------FSTEASNHDVMTWLSAALTYHDTCTDGFA-DVAD 216
           DC+E +++ +D   +++          S      D+ T +S+A+T   TC DGF+ D AD
Sbjct: 132 DCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDAD 191

Query: 217 LAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDLP-VENRRRRRLMESGTQPWAAENGGD- 276
             V+  +      +  M SN+LA+       D+   E R +     +       E  GD 
Sbjct: 192 RKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDL 251

Query: 277 --GGFPQWVSGKDRRLLATPISAIQADIVVAKDGSGKFKTVAEAIDAAPSSSGRRIVIYI 336
              G+P+W+S  DRRLL    S I+AD  VA DGSG F TVA A+ AAP  S +R VI+I
Sbjct: 252 DSDGWPKWLSVGDRRLLQG--STIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIHI 311

Query: 337 KAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIFDKVTTFRTATFAGSGTGIILRD 396
           KAG Y  EN++V +KKTN+MF+GDG+G T+I+G +++ D  TTF +AT A  G   + RD
Sbjct: 312 KAGVY-RENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLARD 371

Query: 397 MTFENTAGASKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIF 456
           +TF+NTAG SKHQAVALR+ +D +  Y+C++  YQDTLYVHSNRQFF +C I GTVDFIF
Sbjct: 372 ITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFIF 431

Query: 457 GNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIIATSDLEASKGSFP 516
           GNA  V Q C+I AR+P +GQKN +TAQ R DPNQNTGI I  C+I  TSDL A KG+FP
Sbjct: 432 GNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFP 491

Query: 517 TYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRVK 576
           TYLGRPWK YSR V M S + D I P GW EW GSFALDTL Y EY+N G GA    RVK
Sbjct: 492 TYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRVK 551

Query: 577 WPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
           W GY+VITS  EA  FT GQFI G  WL STG  FS  L
Sbjct: 552 WKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of CmUC01G000460 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 457.2 bits (1175), Expect = 2.0e-128
Identity = 260/589 (44.14%), Postives = 364/589 (61.80%), Query Frame = 0

Query: 38  SLRRRSRILIISFLSVLLIAASV--VSAAMLVRSHIASSQPNFPHNKPSQAISR-ACSHT 97
           + ++  +++++S    LL  A+V  +SA     +   +  P+      S A+ R +CS T
Sbjct: 13  NFKKNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPS------SHAVLRSSCSST 72

Query: 98  LYPSLCLSSLLSFPGSLSADVHDLVHISLNLTLQHLTKALYSTSQIPISQISNDPLAHSA 157
            YP LC+S++++  G       D++  S+NLT+  +    ++  ++   +    P   +A
Sbjct: 73  RYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTA 132

Query: 158 YEDCMELLNDAVDAF-----SLSLFSTEAS--NH--DVMTWLSAALTYHDTCTDGFA-DV 217
             DC+E +++ +D        L L+ T+ +   H  D+ T +S+A+T  +TC DGF+ D 
Sbjct: 133 LHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDD 192

Query: 218 ADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDL-------PVENRRRRRLMESGTQPW 277
           AD  V+  +      +  M SN+LA+       D+        + +  R+   E+     
Sbjct: 193 ADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTV 252

Query: 278 AAENGGDG-----GFPQWVSGKDRRLLATPISAIQADIVVAKDGSGKFKTVAEAIDAAPS 337
           A +  G G     G+P W+S  DRRLL    S ++AD  VA DGSG FKTVA A+ AAP 
Sbjct: 253 AVDIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAAPE 312

Query: 338 SSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIFDKVTTFRTATFA 397
           +S +R VI+IKAG Y  EN++V +KK N+MF+GDG+  T+I+G +++ D  TTF +AT A
Sbjct: 313 NSNKRYVIHIKAGVY-RENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVA 372

Query: 398 GSGTGIILRDMTFENTAGASKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFREC 457
             G   + RD+TF+NTAG SKHQAVALR+ +D +  Y C+++ YQDTLYVHSNRQFF +C
Sbjct: 373 AVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKC 432

Query: 458 DIYGTVDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIIATS 517
            I GTVDFIFGNA VV Q C+I+AR+P +GQKN +TAQ R DPNQNTGI I  C+I ATS
Sbjct: 433 LIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATS 492

Query: 518 DLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSG 577
           DL++ KGSFPTYLGRPWK YS+ V M S++ D I P GW EW G+FAL+TL Y EY N+G
Sbjct: 493 DLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTG 552

Query: 578 PGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
            GA    RVKW G++VIT+  EA K+T GQFI G  WL STG  FS GL
Sbjct: 553 AGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of CmUC01G000460 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 426.4 bits (1095), Expect = 3.8e-119
Identity = 228/459 (49.67%), Postives = 296/459 (64.49%), Query Frame = 0

Query: 153 SAYEDCMELLNDAVDAFSLSLFSTEASN----------HDVMTWLSAALTYHDTCTDGFA 212
           +A  DC++LL+ A +  S  + ++++ N           D+ TW+SAAL+  DTC DGF 
Sbjct: 79  NAIVDCVDLLDSAAEELSWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDTCLDGFE 138

Query: 213 DVADLAVKDQVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRRRRRLMESGTQPWAAENG 272
               + +K  V   LS +   + N L +         P    + + +     Q     + 
Sbjct: 139 GTNGI-IKKIVAGGLSKVGTTVRNLLTMVHS------PPSKPKPKPIK---AQTMTKAHS 198

Query: 273 GDGGFPQWVSGKDRRLLATPISAIQADIVVAKDGSGKFKTVAEAIDAAPSSSGRRIVIYI 332
           G   FP WV   DR+LL T    + AD VVA DG+G F T+++A+ AAP  S +R VI++
Sbjct: 199 GFSKFPSWVKPGDRKLLQTDNITV-ADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHV 258

Query: 333 KAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIFDKVTTFRTATFAGSGTGIILRD 392
           K G Y  EN+++ +KK N+M VGDG   TVI+G +S  D  TTFR+ATFA SG G I RD
Sbjct: 259 KRGVY-VENVEIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARD 318

Query: 393 MTFENTAGASKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIF 452
           +TF+NTAG  KHQAVA+R   D  V YRC + GYQDTLY HS RQFFREC I GTVDFIF
Sbjct: 319 ITFQNTAGPEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIF 378

Query: 453 GNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIIATSDLEASKGSFP 512
           G+A  VFQSC I A++ +  QKN+ITAQ RKDPN+ TG +I    I A +DL  +  +  
Sbjct: 379 GDATAVFQSCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTA 438

Query: 513 TYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRVK 572
           TYLGRPWKLYSR V+M + M D I+P GWLEW G+FALDTLYYGEYMNSGPGA++ +RVK
Sbjct: 439 TYLGRPWKLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVK 498

Query: 573 WPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
           WPGY V+ ++ EA+ FTV Q I G+ WLPSTG++F AGL
Sbjct: 499 WPGYHVLNTSAEANNFTVSQLIQGNLWLPSTGITFIAGL 525

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038874841.10.0e+0094.21probable pectinesterase/pectinesterase inhibitor 61 [Benincasa hispida][more]
XP_011654619.10.0e+0091.38probable pectinesterase/pectinesterase inhibitor 61 [Cucumis sativus] >KGN49865.... [more]
XP_008437596.10.0e+0091.89PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor... [more]
KAG6579321.11.0e-29988.45putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosp... [more]
XP_022972980.11.5e-29887.64probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9FK054.2e-20363.20Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3... [more]
Q9M3B01.5e-19761.42Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3... [more]
Q425349.8e-12844.73Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2[more]
Q431439.8e-12844.52Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PM... [more]
O490062.8e-12744.14Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME... [more]
Match NameE-valueIdentityDescription
A0A0A0KJG10.0e+0091.38Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G139430 PE=3 SV=1[more]
A0A1S3AUZ60.0e+0091.89Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482964 PE=3 SV=1[more]
A0A6J1IA527.2e-29987.64Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111471502 PE=3 SV=1[more]
A0A6J1E2V12.1e-29887.95Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430323 PE=3 SV=1[more]
A0A6J1H2K18.2e-28785.93Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459120 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G53370.13.0e-20463.20pectin methylesterase PCR fragment F [more]
AT3G49220.11.1e-19861.42Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G53830.16.9e-12944.73pectin methylesterase 2 [more]
AT3G14310.12.0e-12844.14pectin methylesterase 3 [more]
AT3G43270.13.8e-11949.67Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 82..230
e-value: 3.1E-42
score: 156.3
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 86..230
e-value: 2.5E-32
score: 112.3
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 59..232
e-value: 1.1E-34
score: 117.3
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 283..601
e-value: 4.4E-122
score: 409.4
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 289..586
e-value: 7.0E-145
score: 481.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 84..234
e-value: 2.8E-37
score: 130.0
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 85..232
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..39
NoneNo IPR availablePANTHERPTHR31707:SF184PECTINESTERASEcoord: 38..601
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 38..601
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 90..236
e-value: 8.26454E-38
score: 135.258
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 434..443
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 284..600

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmUC01G000460.1CmUC01G000460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity