Homology
BLAST of Clc11G13630 vs. NCBI nr
Match:
XP_038889318.1 ((R)-mandelonitrile lyase 3-like [Benincasa hispida])
HSP 1 Score: 932.6 bits (2409), Expect = 1.7e-267
Identity = 470/532 (88.35%), Postives = 494/532 (92.86%), Query Frame = 0
Query: 4 SIMAAFLMLILVFLSHSQFGLNQDVRYMKFVRDGSEFPEKEEYDYIIVGGGTAGCPLAAT 63
S MAAFL+LILVFL+HSQ GLNQDV YMKFVR+ SEFPEKEEYDYIIVGGGT GCPLAAT
Sbjct: 38 STMAAFLLLILVFLTHSQLGLNQDVSYMKFVRNASEFPEKEEYDYIIVGGGTTGCPLAAT 97
Query: 64 LSKNFSTLLIERGSEPNKYPYVLNEQKLLNVFTVEDDGKNPFNRFISEDGVENIRGRVLG 123
LSKNFSTLLIERGSEP+KYP VLNEQ+LLNVFTVEDDGKNPFNRFISEDGVENIRGR+LG
Sbjct: 98 LSKNFSTLLIERGSEPSKYPSVLNEQELLNVFTVEDDGKNPFNRFISEDGVENIRGRILG 157
Query: 124 GSSMINGGVYSRANPEFFKTQLGVQDFDMEMVEKAYQWVEEAIVYKPISLNPWQAAFRSF 183
G+SMINGGVYSRANPEFFKTQLG+Q+ DMEMVEKAYQWVEEAIVYKP SLN WQAAFR F
Sbjct: 158 GTSMINGGVYSRANPEFFKTQLGMQELDMEMVEKAYQWVEEAIVYKP-SLNLWQAAFRRF 217
Query: 184 LVEGGIEPDNGFDLRDIVGTKISGSLFDENGTRHGAVQLLNKAKPTNLKVAVRATVQRIL 243
LVEGG++PDNGFDLRDIVGTKISGS+FDENG RHGAV+LLNKAKPTNLKVAV+ATVQRIL
Sbjct: 218 LVEGGVQPDNGFDLRDIVGTKISGSIFDENGIRHGAVELLNKAKPTNLKVAVQATVQRIL 277
Query: 244 FSGLSANGVLYSDSKGKLHTTFIREKGEIILSGGALGSPQLLLLSGVGPKSYLSSLNLTV 303
FSGLSA GVLYSDSKGKLHT FIREKGEIILS GALGSPQLLLLSGVGPKSYLSSL L V
Sbjct: 278 FSGLSARGVLYSDSKGKLHTAFIREKGEIILSAGALGSPQLLLLSGVGPKSYLSSLKLRV 337
Query: 304 VLEQPHVGQGMIDNPRFGVNFAPQFPLAFSSSKVIGISQNNSFYFQSIASTTPLSIPPLF 363
VL QPHVGQ M DNPRFG+ A F LAFSSSKVIGISQNNS+YFQSIASTTPLSIPPLF
Sbjct: 338 VLNQPHVGQFMSDNPRFGIILALSFRLAFSSSKVIGISQNNSYYFQSIASTTPLSIPPLF 397
Query: 364 SILPPNSTSLTTTSLATIGGKFSKIASMGSLRLNSSTNVNKNPIVRFNYYSDPNDIAMCV 423
SI PPNSTSLTTTSLATIGGKFSKI+S GSL LNSST+V NPIVRFNYYS P+D+A CV
Sbjct: 398 SIFPPNSTSLTTTSLATIGGKFSKISSFGSLHLNSSTDVKNNPIVRFNYYSHPDDLAKCV 457
Query: 424 RGVRKVGDFLQTQTIENIKTRDLEGNKRIQFVGLPLPENLSDDASVGEFCKKTVTSYWHY 483
RGVRKVGDFL+T TIENIKTRDLEGNK +QFVGLPLPENLSDD VGEFCKKTVTSYWHY
Sbjct: 458 RGVRKVGDFLKTPTIENIKTRDLEGNKTLQFVGLPLPENLSDDTLVGEFCKKTVTSYWHY 517
Query: 484 HGGCLVGKVVDGNYSVIGIKNLRVLDGSTFAVSPGSNPTATLMMLARKLKLK 536
HGGCLVGKVVD NYSVIGIKNLRVLDGSTFAVSPGSNPTATLMMLAR + L+
Sbjct: 518 HGGCLVGKVVDSNYSVIGIKNLRVLDGSTFAVSPGSNPTATLMMLARYVGLR 568
BLAST of Clc11G13630 vs. NCBI nr
Match:
XP_011648921.2 ((R)-mandelonitrile lyase 1 [Cucumis sativus])
HSP 1 Score: 906.0 bits (2340), Expect = 1.7e-259
Identity = 455/536 (84.89%), Postives = 491/536 (91.60%), Query Frame = 0
Query: 1 MEN-SIMAAFLMLILVFLSHSQFGLNQDVRYMKFVRDGSEFPEKEEYDYIIVGGGTAGCP 60
MEN S M AFL+LILVFLSHSQ GLNQDV YMKFV+D SEFP KEEYDYIIVGGGT GCP
Sbjct: 1 MENYSTMVAFLLLILVFLSHSQLGLNQDVSYMKFVQDASEFPIKEEYDYIIVGGGTTGCP 60
Query: 61 LAATLSKNFSTLLIERGSEPNKYPYVLNEQKLLNVFTVEDDGKNPFNRFISEDGVENIRG 120
LAATLSK FS LLIERGSEP+KYP VLNEQ+LLNVFTVEDDGKNPFNRFISEDGVENIRG
Sbjct: 61 LAATLSKKFSVLLIERGSEPSKYPSVLNEQQLLNVFTVEDDGKNPFNRFISEDGVENIRG 120
Query: 121 RVLGGSSMINGGVYSRANPEFFKTQLGVQDFDMEMVEKAYQWVEEAIVYKPISLNPWQAA 180
RVLGGSSMINGGVYSRA+PEFF+TQLG+Q+ DMEMVEKAY+WVEEAIV+KP SLN WQ A
Sbjct: 121 RVLGGSSMINGGVYSRADPEFFRTQLGMQELDMEMVEKAYEWVEEAIVFKP-SLNLWQGA 180
Query: 181 FRSFLVEGGIEPDNGFDLRDIVGTKISGSLFDENGTRHGAVQLLNKAKPTNLKVAVRATV 240
FR FLVEGG+EPDNGFDLRD VGTKISGS+FDENGTRHGAV+LLNKA+PTNLKVAV+A V
Sbjct: 181 FRRFLVEGGVEPDNGFDLRDDVGTKISGSIFDENGTRHGAVELLNKAQPTNLKVAVQAIV 240
Query: 241 QRILFSGLSANGVLYSDSKGKLHTTFIREKGEIILSGGALGSPQLLLLSGVGPKSYLSSL 300
QRILFSGLSANGVLYSDSKGKLHT +IR++GEII+S GA+GSPQLLLLSG+GPKSYLSSL
Sbjct: 241 QRILFSGLSANGVLYSDSKGKLHTAYIRKEGEIIVSAGAIGSPQLLLLSGIGPKSYLSSL 300
Query: 301 NLTVVLEQPHVGQGMIDNPRFGVNFAPQFPLAFSSSKVIGISQNNSFYFQSIASTTPLSI 360
L VVL QPHVGQ M+D PRFG A FPL+F+SSKVIGISQN +FYFQSIASTTPLSI
Sbjct: 301 QLPVVLHQPHVGQSMMDYPRFGYVLAWPFPLSFTSSKVIGISQNKTFYFQSIASTTPLSI 360
Query: 361 PPLFSILPPNSTSLTTTSLATIGGKFSKIASMGSLRLNSSTNVNKNPIVRFNYYSDPNDI 420
PPLFSI PPNSTSLTTTSLATIGGKFSK+AS GSLRLNSS + NPIVRFNYYS P D+
Sbjct: 361 PPLFSIFPPNSTSLTTTSLATIGGKFSKVASTGSLRLNSSADAASNPIVRFNYYSHPADV 420
Query: 421 AMCVRGVRKVGDFLQTQTIENIKTRDLEGNKRIQFVGLPLPENLSDDASVGEFCKKTVTS 480
AMCV+GVRKVG FL+TQT+ENIKTRDLEGNK IQFVGLPLP NLSDD++VGEFCKKTVT
Sbjct: 421 AMCVKGVRKVGGFLKTQTVENIKTRDLEGNKTIQFVGLPLPGNLSDDSAVGEFCKKTVTL 480
Query: 481 YWHYHGGCLVGKVVDGNYSVIGIKNLRVLDGSTFAVSPGSNPTATLMMLARKLKLK 536
YWHYHGGCLVGKVVDGNYSVIG+KNLRVLDGSTFAVSPGSNPTATLMMLAR + L+
Sbjct: 481 YWHYHGGCLVGKVVDGNYSVIGVKNLRVLDGSTFAVSPGSNPTATLMMLARYVGLQ 535
BLAST of Clc11G13630 vs. NCBI nr
Match:
XP_023546914.1 ((R)-mandelonitrile lyase 1-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 835.1 bits (2156), Expect = 3.7e-238
Identity = 418/543 (76.98%), Postives = 475/543 (87.48%), Query Frame = 0
Query: 1 MENSIMAAFLMLILVFLSHSQFGL--------NQDVRYMKFVRDGSEFPEKEEYDYIIVG 60
MENS M FL+LIL+FLSHSQ L NQD+ YMKFVRDG++FPEKEEYDYIIVG
Sbjct: 1 MENSSMPTFLLLILLFLSHSQLLLSISTNTQPNQDISYMKFVRDGNDFPEKEEYDYIIVG 60
Query: 61 GGTAGCPLAATLSKNFSTLLIERGSEPNKYPYVLNEQKLLNVFTVEDDGKNPFNRFISED 120
GGTAGCPLAATLSKNFSTLLIERGSEPNKYP VL EQ LLNVFTVEDDGKNP+NRF S++
Sbjct: 61 GGTAGCPLAATLSKNFSTLLIERGSEPNKYPSVLKEQALLNVFTVEDDGKNPYNRFTSKE 120
Query: 121 GVENIRGRVLGGSSMINGGVYSRANPEFFKTQLGVQDFDMEMVEKAYQWVEEAIVYKPIS 180
GV+N+RGRVLGG+SMINGGVYS+ N +FFKTQLGVQ FDMEMVEKAY+WVEEA+VY+P S
Sbjct: 121 GVQNLRGRVLGGTSMINGGVYSKPNTDFFKTQLGVQKFDMEMVEKAYKWVEEALVYRP-S 180
Query: 181 LNPWQAAFRSFLVEGGIEPDNGFDLRDIVGTKISGSLFDENGTRHGAVQLLNKAKPTNLK 240
LN WQ AFR LVEGGI PDNGF+LRD+VGTKI+GS+FD+NG RHGAV+LLNKA+P NLK
Sbjct: 181 LNAWQNAFRRILVEGGIVPDNGFELRDVVGTKITGSIFDDNGNRHGAVELLNKARPRNLK 240
Query: 241 VAVRATVQRILFSGLSANGVLYSDSKGKLHTTFIREKGEIILSGGALGSPQLLLLSGVGP 300
VA+RATV+RI+FSG SANGVLYSDSKGK HT IR+ GEII+S GA+GSPQLLLLSGVGP
Sbjct: 241 VAIRATVERIIFSGSSANGVLYSDSKGKQHTAVIRKNGEIIVSAGAIGSPQLLLLSGVGP 300
Query: 301 KSYLSSLNLTVVLEQPHVGQGMIDNPRFGVNFAPQFPLAFSSSKVIGISQNNSFYFQSIA 360
KS+LSSLNL ++L PHVG+ M DNPR GV FPL F+SSKVIGIS NN++YFQSIA
Sbjct: 301 KSHLSSLNLPLILHNPHVGEFMKDNPRVGVVPILPFPLDFTSSKVIGISTNNTYYFQSIA 360
Query: 361 STTPLSIPPLFSILPPNSTSLTTTSLATIGGKFSKIASMGSLRLNSSTNVNKNPIVRFNY 420
ST+P SIPP FS+LPPNST++ S+ATIGGKFSK+ S+GSLRLNSST+V +PIVRFNY
Sbjct: 361 STSPFSIPPSFSLLPPNSTTV-NLSIATIGGKFSKLTSVGSLRLNSSTDVKTSPIVRFNY 420
Query: 421 YSDPNDIAMCVRGVRKVGDFLQTQTIENIKTRDLEGNKRIQFVGLPLPENLSDDASVGEF 480
YS+P DI +CV GVRKVGDFL+TQ I+NIKTRDLEGN+ IQF+G PLPENLSD +SV EF
Sbjct: 421 YSNPADIQLCVGGVRKVGDFLKTQIIQNIKTRDLEGNRVIQFLGPPLPENLSDYSSVAEF 480
Query: 481 CKKTVTSYWHYHGGCLVGKVVDGNYSVIGIKNLRVLDGSTFAVSPGSNPTATLMMLARKL 536
CK+TVT+YWHYHGGCLVGKVVDGNYSVIG+KNLRVLDGSTFAVSPGSNP ATLMML R +
Sbjct: 481 CKRTVTTYWHYHGGCLVGKVVDGNYSVIGLKNLRVLDGSTFAVSPGSNPIATLMMLGRYV 540
BLAST of Clc11G13630 vs. NCBI nr
Match:
XP_022929724.1 ((R)-mandelonitrile lyase 1-like [Cucurbita moschata])
HSP 1 Score: 834.3 bits (2154), Expect = 6.2e-238
Identity = 417/543 (76.80%), Postives = 474/543 (87.29%), Query Frame = 0
Query: 1 MENSIMAAFLMLILVFLSHSQFGL--------NQDVRYMKFVRDGSEFPEKEEYDYIIVG 60
MENS M FL+L+L+FLSHSQ L NQD+ YMKFV DG++FPEKEEYDYIIVG
Sbjct: 1 MENSTMPTFLLLVLLFLSHSQLLLLISTNTRPNQDISYMKFVHDGNDFPEKEEYDYIIVG 60
Query: 61 GGTAGCPLAATLSKNFSTLLIERGSEPNKYPYVLNEQKLLNVFTVEDDGKNPFNRFISED 120
GGTAGCPLAATLSKNFSTLLIERGSEPNKYP VL EQ LLNVFTVEDDGKNP+NRF S++
Sbjct: 61 GGTAGCPLAATLSKNFSTLLIERGSEPNKYPSVLKEQALLNVFTVEDDGKNPYNRFTSKE 120
Query: 121 GVENIRGRVLGGSSMINGGVYSRANPEFFKTQLGVQDFDMEMVEKAYQWVEEAIVYKPIS 180
GV+N+RGRVLGG+SMINGGVYS+ N +FFKTQLGVQ FDMEMVEKAY+WVEEA+VY+P S
Sbjct: 121 GVQNLRGRVLGGTSMINGGVYSKPNTDFFKTQLGVQKFDMEMVEKAYKWVEEALVYRP-S 180
Query: 181 LNPWQAAFRSFLVEGGIEPDNGFDLRDIVGTKISGSLFDENGTRHGAVQLLNKAKPTNLK 240
LN WQ AFR LVEGGI PDNGF+LRD+VGTKI+GS+FDENG RHGAV+LLNKA+P NLK
Sbjct: 181 LNAWQNAFRRILVEGGIVPDNGFELRDVVGTKITGSIFDENGNRHGAVELLNKARPGNLK 240
Query: 241 VAVRATVQRILFSGLSANGVLYSDSKGKLHTTFIREKGEIILSGGALGSPQLLLLSGVGP 300
VA+RATV+RI+FSG SA+ VLYSDSKGKLHT + + GEIILS GA+GSPQLLLLSGVGP
Sbjct: 241 VAIRATVERIIFSGSSASEVLYSDSKGKLHTAVVHKNGEIILSAGAIGSPQLLLLSGVGP 300
Query: 301 KSYLSSLNLTVVLEQPHVGQGMIDNPRFGVNFAPQFPLAFSSSKVIGISQNNSFYFQSIA 360
KS+LSSLN+ ++L PHVG+ M DNPR GV FPL F+SSKVIGIS NN++YFQSIA
Sbjct: 301 KSHLSSLNIPLILHNPHVGEFMKDNPRVGVVPILPFPLDFTSSKVIGISNNNTYYFQSIA 360
Query: 361 STTPLSIPPLFSILPPNSTSLTTTSLATIGGKFSKIASMGSLRLNSSTNVNKNPIVRFNY 420
ST+P SIPP FS+LPPNSTS+ T +ATIGGKFSK+AS+GSLRLNSST+V NPIVRFNY
Sbjct: 361 STSPFSIPPSFSLLPPNSTSVNLT-VATIGGKFSKLASVGSLRLNSSTDVKTNPIVRFNY 420
Query: 421 YSDPNDIAMCVRGVRKVGDFLQTQTIENIKTRDLEGNKRIQFVGLPLPENLSDDASVGEF 480
YS+P DI +CV GVRKVGDFL+TQ I+NIKTRDLEGN+ IQF+G PLPENLSD +SV EF
Sbjct: 421 YSNPADIQLCVGGVRKVGDFLKTQIIQNIKTRDLEGNRVIQFLGPPLPENLSDYSSVAEF 480
Query: 481 CKKTVTSYWHYHGGCLVGKVVDGNYSVIGIKNLRVLDGSTFAVSPGSNPTATLMMLARKL 536
CK+TVT+YWHYHGGCLVGKVVDGNYSVIG+KNLRVLDGSTFAVSPGSNP ATLMML R +
Sbjct: 481 CKRTVTTYWHYHGGCLVGKVVDGNYSVIGLKNLRVLDGSTFAVSPGSNPIATLMMLGRYV 540
BLAST of Clc11G13630 vs. NCBI nr
Match:
XP_022997400.1 ((R)-mandelonitrile lyase 1-like isoform X1 [Cucurbita maxima])
HSP 1 Score: 832.0 bits (2148), Expect = 3.1e-237
Identity = 416/543 (76.61%), Postives = 472/543 (86.92%), Query Frame = 0
Query: 1 MENSIMAAFLMLILVFLSHSQFGL--------NQDVRYMKFVRDGSEFPEKEEYDYIIVG 60
MENS M FL+LIL+ LSHSQ L N+D+ YMKFVRDG++FPEKEEYDYIIVG
Sbjct: 1 MENSTMPTFLLLILLLLSHSQLRLLISTNTQPNEDISYMKFVRDGNDFPEKEEYDYIIVG 60
Query: 61 GGTAGCPLAATLSKNFSTLLIERGSEPNKYPYVLNEQKLLNVFTVEDDGKNPFNRFISED 120
GGTAGCPLAATLSKNFSTLLIERGSEPNKYP VL EQ LLNVFTVEDDGKNPFNRF+S++
Sbjct: 61 GGTAGCPLAATLSKNFSTLLIERGSEPNKYPSVLKEQALLNVFTVEDDGKNPFNRFVSKE 120
Query: 121 GVENIRGRVLGGSSMINGGVYSRANPEFFKTQLGVQDFDMEMVEKAYQWVEEAIVYKPIS 180
GV+N+RGRVLGG+SMINGGVYS+ N +FFKTQLGVQ FDMEMVEKAY+WVEEA+VY+P
Sbjct: 121 GVQNLRGRVLGGTSMINGGVYSKPNTDFFKTQLGVQKFDMEMVEKAYKWVEEALVYRP-R 180
Query: 181 LNPWQAAFRSFLVEGGIEPDNGFDLRDIVGTKISGSLFDENGTRHGAVQLLNKAKPTNLK 240
LN WQ AFR LVEGGI PDNGF+LRD+VGTKI+GS+FDENG RHGA +LLNKA+P NLK
Sbjct: 181 LNAWQNAFRRVLVEGGIVPDNGFELRDVVGTKIAGSIFDENGNRHGAEELLNKARPGNLK 240
Query: 241 VAVRATVQRILFSGLSANGVLYSDSKGKLHTTFIREKGEIILSGGALGSPQLLLLSGVGP 300
VA+RATV+RI+FSG SA GVLYSDSKGKLHT IR+ GEII+S GA+GSPQLLLLSGVGP
Sbjct: 241 VAIRATVERIIFSGSSATGVLYSDSKGKLHTAVIRKNGEIIVSAGAIGSPQLLLLSGVGP 300
Query: 301 KSYLSSLNLTVVLEQPHVGQGMIDNPRFGVNFAPQFPLAFSSSKVIGISQNNSFYFQSIA 360
KS+LSSLNL ++L PHVGQ M DNPR GV +PL F+SSKVIGIS NN++YFQSIA
Sbjct: 301 KSHLSSLNLPLILHNPHVGQFMKDNPRVGVIPILPYPLDFTSSKVIGISNNNTYYFQSIA 360
Query: 361 STTPLSIPPLFSILPPNSTSLTTTSLATIGGKFSKIASMGSLRLNSSTNVNKNPIVRFNY 420
+T+P SIPP FS+LPPNSTS+ T +ATIGGKFSK+ S+GSLRLNSST+V PIVRFNY
Sbjct: 361 TTSPFSIPPSFSLLPPNSTSVNLT-IATIGGKFSKLTSVGSLRLNSSTDVKTTPIVRFNY 420
Query: 421 YSDPNDIAMCVRGVRKVGDFLQTQTIENIKTRDLEGNKRIQFVGLPLPENLSDDASVGEF 480
YS+P DI +CV GVRKVGDFL+TQ I+NIKTRDLEGN+ IQF+G PLPENLSD +SV EF
Sbjct: 421 YSNPADIQLCVGGVRKVGDFLKTQIIQNIKTRDLEGNRVIQFLGPPLPENLSDYSSVAEF 480
Query: 481 CKKTVTSYWHYHGGCLVGKVVDGNYSVIGIKNLRVLDGSTFAVSPGSNPTATLMMLARKL 536
CK+TVT+YWHYHGGCLVGKVVDGNYSVIG+KNLRVLDGSTFAVSPGSNP ATLMML R +
Sbjct: 481 CKRTVTTYWHYHGGCLVGKVVDGNYSVIGLKNLRVLDGSTFAVSPGSNPIATLMMLGRYV 540
BLAST of Clc11G13630 vs. ExPASy Swiss-Prot
Match:
P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)
HSP 1 Score: 512.3 bits (1318), Expect = 7.1e-144
Identity = 263/546 (48.17%), Postives = 362/546 (66.30%), Query Frame = 0
Query: 1 MENSIMAAFLMLILVFLSHSQFG--------LNQDVRYMKFVRDGSEFPEKEEYDYIIVG 60
ME S M+A L+++ +F+ Q+ N D Y++F D ++ + YDY+IVG
Sbjct: 1 MEKSTMSAILLVLHLFVLLLQYSEVHSLATTSNHDFSYLRFAYDATDLELEGSYDYVIVG 60
Query: 61 GGTAGCPLAATLSKNFSTLLIERGSEPNKYPYVLNEQKLLNVFTVEDDGKNPFNRFISED 120
GGT+GCPLAATLS+ + L++ERGS P YP VL + EDDGK P RF+SED
Sbjct: 61 GGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSED 120
Query: 121 GVENIRGRVLGGSSMINGGVYSRANPEFFKTQLGVQDFDMEMVEKAYQWVEEAIVYKPIS 180
G++N+RGRVLGG+SMIN GVY+RAN + GV D+DM++V K Y+WVE+ IV+KP +
Sbjct: 121 GIDNVRGRVLGGTSMINAGVYARANTSIYSAS-GV-DWDMDLVNKTYEWVEDTIVFKP-N 180
Query: 181 LNPWQAAFRSFLVEGGIEPDNGFDLRDIVGTKISGSLFDENGTRHGAVQLLNKAKPTNLK 240
PWQ+ + +E G++P++GF L GT+I+GS FD GTRH A +LLNK NL+
Sbjct: 181 YQPWQSVTGTAFLEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLR 240
Query: 241 VAVRATVQRILFS---GLSANGVLYSDSKGKLHTTFIREKGEIILSGGALGSPQLLLLSG 300
V V A+V++I+FS GL+A GV+Y DS G H F+R KGE+I+S G +G+PQLLLLSG
Sbjct: 241 VGVHASVEKIIFSNAPGLTATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSG 300
Query: 301 VGPKSYLSSLNLTVVLEQPHVGQGMIDNPRFGVNFAPQFPLAFSSSKVIGISQNNSFYFQ 360
VGP+SYLSSLN+ VVL P+VGQ + DNPR +N P P+ + V+GIS N FY Q
Sbjct: 301 VGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGIS--NDFY-Q 360
Query: 361 SIASTTPLSIPPLFSILPPNSTSLTTTSLATIGGKFSKIASMGSLRLNSSTNVNKNPIVR 420
S+ P + PP FS P S L ++ A K + S GSL L SS+NV +P V+
Sbjct: 361 CSFSSLPFTTPP-FSFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVK 420
Query: 421 FNYYSDPNDIAMCVRGVRKVGDFLQTQTIENIKTRDLEGNKRIQFVGLPLPENLSDDASV 480
FNYYS+P D++ CV G++K+G+ L T ++ K DL G + +G+PLP++ +DDA+
Sbjct: 421 FNYYSNPTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDDAAF 480
Query: 481 GEFCKKTVTSYWHYHGGCLVGKVVDGNYSVIGIKNLRVLDGSTFAVSPGSNPTATLMMLA 536
FC+++V SYWHYHGGCLVGKV+DG++ V GI LRV+DGSTF +P S+P +ML
Sbjct: 481 ETFCRESVASYWHYHGGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHPQGFYLMLG 539
BLAST of Clc11G13630 vs. ExPASy Swiss-Prot
Match:
P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)
HSP 1 Score: 506.5 bits (1303), Expect = 3.9e-142
Identity = 268/573 (46.77%), Postives = 372/573 (64.92%), Query Frame = 0
Query: 1 MENSIMAAFLMLILVFLSHSQFG--------LNQDVRYMKFVRDGSEFPEKEEYDYIIVG 60
M S M+A L+++ +F+ H Q+ + D Y+ FV D ++ + YDYIIVG
Sbjct: 1 MVKSTMSAVLLVLHIFVLHLQYSEVQSLANTSSHDFSYLSFVYDATDPELEGSYDYIIVG 60
Query: 61 GGTAGCPLAATLSKNFSTLLIERGSEPNKYPYVLNEQKLLNVFTVEDDGKNPFNRFISED 120
GGTAGCPLAATLS N+S L++ERGS P +YP +L + EDDGK P RF+SED
Sbjct: 61 GGTAGCPLAATLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFVSED 120
Query: 121 GVENIRGRVLGGSSMINGGVYSRANPEFFKTQLGVQDFDMEMVEKAYQWVEEAIVYKPIS 180
G++N+RGRVLGG+SMIN GVY RAN FF Q G+ ++DM++V + Y+WVE+ IV++P S
Sbjct: 121 GIDNVRGRVLGGTSMINAGVYVRANTSFF-NQTGI-EWDMDLVNQTYEWVEDTIVFEPDS 180
Query: 181 LNPWQAAFRSFLVEGGIEPDNGFDLRDIVGTKISGSLFDENGTRHGAVQLLNKAKPTNLK 240
WQ + +E GI P+NGF + + GT+++GS FD NGTRH + +LLNK P NL+
Sbjct: 181 -QTWQTVIGTAYLEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLR 240
Query: 241 VAVRATVQRILF----SGLSANGVLYSDSKGKLHTTFIREKGEIILSGGALGSPQLLLLS 300
VAV+A V++I+F SG++A GV+Y+DS G H F+R +GE+ILS G +GSPQLLLLS
Sbjct: 241 VAVQAAVEKIIFSSNTSGVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLS 300
Query: 301 GVGPKSYLSSLNLTVVLEQPHVGQGMIDNPRFGVNFAPQFPLAFSSSKVIGISQNNSFYF 360
GVGP+SYL+SLN++VV P+VGQ + DNPR +N P P+ S+ V+GI+ S ++
Sbjct: 301 GVGPESYLTSLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGIT---SDFY 360
Query: 361 QSIASTTPLSIPPLFSILPPNSTSLTTTSLATIGGKFSKIASMGSLRLNSSTNVNKNPIV 420
Q S+ P PP FS P S L + A I K S G++ LNSS++V P V
Sbjct: 361 QCSISSLPFDTPP-FSFFPTTSYPLPNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNV 420
Query: 421 RFNYYSDPNDIAMCVRGVRKVGDFLQTQTIENIKTRDLEGNKRIQFVGLPLPENLSDDAS 480
+FNYYS+ D++ CV G++K+G+ L T +E K DL G +G+PLPEN +DDA+
Sbjct: 421 KFNYYSNLTDLSHCVSGMKKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAA 480
Query: 481 VGEFCKKTVTSYWHYHGGCLVGKVVDGNYSVIGIKNLRVLDGSTFAVSPGSNPTATLMML 540
FC+++V SYWHYHGGCLVGKV+D + V GI LRV+DGSTF +P S+P +ML
Sbjct: 481 FETFCRESVASYWHYHGGCLVGKVLDDGFRVTGINALRVVDGSTFPSTPASHPQGFYLML 540
Query: 541 ARKLKLKFRILLHAQNDE-KDINF---FLDKPQ 558
R + ++ A D +++ F LD P+
Sbjct: 541 GRYMGIQILQERSASEDAIRNLGFQENILDSPK 566
BLAST of Clc11G13630 vs. ExPASy Swiss-Prot
Match:
Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)
HSP 1 Score: 505.4 bits (1300), Expect = 8.7e-142
Identity = 260/546 (47.62%), Postives = 359/546 (65.75%), Query Frame = 0
Query: 1 MENSIMAAFLMLILVFLSHSQFG--------LNQDVRYMKFVRDGSEFPEKEEYDYIIVG 60
ME S M+A L+++ +F+ H Q+ + D Y+ F D ++ + YDY+IVG
Sbjct: 1 MEKSTMSAILLVLYIFVLHLQYSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVG 60
Query: 61 GGTAGCPLAATLSKNFSTLLIERGSEPNKYPYVLNEQKLLNVFTVEDDGKNPFNRFISED 120
GGT+GCPLAATLS+ + L++ERGS P YP VL + EDDGK P RF+SED
Sbjct: 61 GGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSED 120
Query: 121 GVENIRGRVLGGSSMINGGVYSRANPEFFKTQLGVQDFDMEMVEKAYQWVEEAIVYKPIS 180
G++N+RGRVLGG+S+IN GVY+RAN + GV D+DM++V + Y+WVE+ IVYKP S
Sbjct: 121 GIDNVRGRVLGGTSIINAGVYARANTSIYSAS-GV-DWDMDLVNQTYEWVEDTIVYKPNS 180
Query: 181 LNPWQAAFRSFLVEGGIEPDNGFDLRDIVGTKISGSLFDENGTRHGAVQLLNKAKPTNLK 240
WQ+ ++ +E G+ P++GF L GT+I+GS FD GTRH A +LLNK NL+
Sbjct: 181 -QSWQSVTKTAFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLR 240
Query: 241 VAVRATVQRILFS---GLSANGVLYSDSKGKLHTTFIREKGEIILSGGALGSPQLLLLSG 300
V V A+V++I+FS GL+A GV+Y DS G H F+R KGE+I+S G +G+PQLLLLSG
Sbjct: 241 VGVHASVEKIIFSNAPGLTATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSG 300
Query: 301 VGPKSYLSSLNLTVVLEQPHVGQGMIDNPRFGVNFAPQFPLAFSSSKVIGISQNNSFYFQ 360
VGP+SYLSSLN+ VVL P+VGQ + DNPR +N P P+ + V+GIS N FY Q
Sbjct: 301 VGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGIS--NDFY-Q 360
Query: 361 SIASTTPLSIPPLFSILPPNSTSLTTTSLATIGGKFSKIASMGSLRLNSSTNVNKNPIVR 420
S+ P + PP F P S L ++ A K + S GSL L SS+NV +P V+
Sbjct: 361 CSFSSLPFTTPP-FGFFPSASYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVK 420
Query: 421 FNYYSDPNDIAMCVRGVRKVGDFLQTQTIENIKTRDLEGNKRIQFVGLPLPENLSDDASV 480
FNYYS+ D++ CV G++K+G+ L T ++ K DL G + +G+PLP++ +DDA+
Sbjct: 421 FNYYSNLTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAF 480
Query: 481 GEFCKKTVTSYWHYHGGCLVGKVVDGNYSVIGIKNLRVLDGSTFAVSPGSNPTATLMMLA 536
FC+++V SYWHYHGGCLVGKV+DG++ V GI LRV+DGSTF +P S+P +ML
Sbjct: 481 ETFCRESVASYWHYHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHPQGFYLMLG 539
BLAST of Clc11G13630 vs. ExPASy Swiss-Prot
Match:
O82784 ((R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1)
HSP 1 Score: 495.7 bits (1275), Expect = 6.9e-139
Identity = 262/548 (47.81%), Postives = 355/548 (64.78%), Query Frame = 0
Query: 1 MENSIMAAFLMLILVFLSHSQF---------GLNQDVRYMKFVRDGSEFPEKEEYDYIIV 60
ME S M+A ++++ + + H Q+ D Y+KFV + + + YDYIIV
Sbjct: 1 MEKSTMSAVVLVLNLLVLHLQYSEVHSLANTSSEHDFGYLKFVYNAVDLELEGSYDYIIV 60
Query: 61 GGGTAGCPLAATLSKNFSTLLIERGSEPNKYPYVLNEQKLLNVFTVEDDGKNPFNRFISE 120
GGGT+GCPLAATLS N+S L++ERG+ +YP L +DDGK P RF+SE
Sbjct: 61 GGGTSGCPLAATLSANYSVLVLERGTIATEYPNTLTVDGFAYNLQQQDDGKTPVERFVSE 120
Query: 121 DGVENIRGRVLGGSSMINGGVYSRANPEFFKTQLGVQDFDMEMVEKAYQWVEEAIVYKPI 180
DG++N+R R+LGG+++IN GVY+RAN F+ GV ++D+++V +AY+WVE+AIVYKP
Sbjct: 121 DGIDNVRSRILGGTTIINAGVYARANESFYNNS-GV-EWDLDLVNEAYEWVEDAIVYKP- 180
Query: 181 SLNPWQAAFRSFLVEGGIEPDNGFDLRDIVGTKISGSLFDENGTRHGAVQLLNKAKPTNL 240
S WQ+ + +E G+ PDNGF L GT+++GS FD +GTRH + +LLNK P NL
Sbjct: 181 SNQSWQSITGTAFLEAGVHPDNGFGLVHEEGTRLTGSTFDNSGTRHASDELLNKGDPDNL 240
Query: 241 KVAVRATVQRILF----SGLSANGVLYSDSKGKLHTTFIREKGEIILSGGALGSPQLLLL 300
KVAV A VQ+I+F SGL+A GV+Y+DS G H + KGE+ILS G LG+PQLLLL
Sbjct: 241 KVAVEAAVQKIIFSTESSGLTAVGVVYTDSNGTSHRALVSGKGEVILSAGTLGTPQLLLL 300
Query: 301 SGVGPKSYLSSLNLTVVLEQPHVGQGMIDNPRFGVNFAPQFPLAFSSSKVIGISQNNSFY 360
SGVGP+SYL+SLN++VV P+VGQ + DNPR +N P P+ S+ V+GI+ S +
Sbjct: 301 SGVGPESYLTSLNISVVASHPYVGQYVNDNPRNFINILPPNPIEPSTVTVLGIT---SDF 360
Query: 361 FQSIASTTPLSIPPLFSILPPNSTSLTTTSLATIGGKFSKIASMGSLRLNSSTNVNKNPI 420
+Q S+ P PP FS+ P S L + A I K S GSL L SS+NV+ P
Sbjct: 361 YQCSLSSLPFDTPP-FSLFPTTSYPLPNQTFAHIVSKVPGPLSAGSLTLQSSSNVSVAPN 420
Query: 421 VRFNYYSDPNDIAMCVRGVRKVGDFLQTQTIENIKTRDLEGNKRIQFVGLPLPENLSDDA 480
V+FNY SDP D+ CV G++K+G FL T ++ K DL G +G PLPEN +DDA
Sbjct: 421 VKFNYCSDPVDLTHCVSGMKKIGVFLSTDALKPYKVDDLPGIDGFNILGTPLPENQTDDA 480
Query: 481 SVGEFCKKTVTSYWHYHGGCLVGKVVDGNYSVIGIKNLRVLDGSTFAVSPGSNPTATLMM 536
+ +FC+ TV SYWHYHGG +VGKV+DGN+ V GI LRV+DGSTF +P S+P +M
Sbjct: 481 AFEKFCRDTVASYWHYHGGAIVGKVIDGNFRVTGINALRVVDGSTFPATPASHPQGFYLM 540
BLAST of Clc11G13630 vs. ExPASy Swiss-Prot
Match:
O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)
HSP 1 Score: 488.8 bits (1257), Expect = 8.4e-137
Identity = 258/547 (47.17%), Postives = 356/547 (65.08%), Query Frame = 0
Query: 1 MENSIMAAFLMLILVFLSHSQFG--------LNQDVRYMKFVRDGSEFPEKEEYDYIIVG 60
ME S M+ L ++ + + H Q+ D Y+KFV + ++ + YDYI++G
Sbjct: 1 MEKSTMSVILFVLHLLVLHLQYSEVHSLANTSAHDFSYLKFVYNATDTSLEGSYDYIVIG 60
Query: 61 GGTAGCPLAATLSKNFSTLLIERGSEPNKYPYVLNEQKLLNVFTVEDDGKNPFNRFISED 120
GGT+GCPLAATLS+ + LL+ERG+ +YP L +DDGK P RF+SED
Sbjct: 61 GGTSGCPLAATLSEKYKVLLLERGTIATEYPNTLTADGFAYNLQQQDDGKTPVERFVSED 120
Query: 121 GVENIRGRVLGGSSMINGGVYSRANPEFFKTQLGVQDFDMEMVEKAYQWVEEAIVYKPIS 180
G++N+R R+LGG+++IN GVY+RAN F+ +Q G+ ++D+++V K Y+WVE+AIV KP +
Sbjct: 121 GIDNVRARILGGTTIINAGVYARANISFY-SQTGI-EWDLDLVNKTYEWVEDAIVVKP-N 180
Query: 181 LNPWQAAFRSFLVEGGIEPDNGFDLRDIVGTKISGSLFDENGTRHGAVQLLNKAKPTNLK 240
WQ+ +E GI PDNGF L GT+++GS FD NGTRH A +LLNK P NL
Sbjct: 181 NQSWQSVIGEGFLEAGILPDNGFSLDHEAGTRLTGSTFDNNGTRHAADELLNKGDPNNLL 240
Query: 241 VAVRATVQRILF----SGLSANGVLYSDSKGKLHTTFIREKGEIILSGGALGSPQLLLLS 300
VAV+A+V++ILF S LSA GV+Y+DS G H F+R GE+I+S G +G+PQLLLLS
Sbjct: 241 VAVQASVEKILFSSNTSNLSAIGVIYTDSDGNSHQAFVRGNGEVIVSAGTIGTPQLLLLS 300
Query: 301 GVGPKSYLSSLNLTVVLEQPHVGQGMIDNPRFGVNFAPQFPLAFSSSKVIGISQNNSFYF 360
GVGP+SYLSSLN+TVV P+VGQ + +NPR +N P P+ S V+GI S Y+
Sbjct: 301 GVGPESYLSSLNITVVQPNPYVGQFLYNNPRNFINNFPPNPIEASVVTVLGI---RSDYY 360
Query: 361 QSIASTTPLSIPPLFSILPPNSTSLTTTSLATIGGKFSKIASMGSLRLNSSTNVNKNPIV 420
Q S+ P S PP FS+ P S L ++ A I + S GS+ LNSS++V P +
Sbjct: 361 QVSLSSLPFSTPP-FSLFPTTSYPLPNSTFAHIVSQVPGPLSHGSVTLNSSSDVRIAPNI 420
Query: 421 RFNYYSDPNDIAMCVRGVRKVGDFLQTQTIENIKTRDLEGNKRIQFVGLPLPENLSDDAS 480
+FNYYS+ D+A CV G++K+GD L+T+ +E K RD+ G ++G+PLPEN +DDAS
Sbjct: 421 KFNYYSNSTDLANCVSGMKKLGDLLRTKALEPYKARDVLGIDGFNYLGVPLPENQTDDAS 480
Query: 481 VGEFCKKTVTSYWHYHGGCLVGKVVDGNYSVIGIKNLRVLDGSTFAVSPGSNPTATLMML 536
FC V SYWHYHGG LVGKV+D ++ V+GIK LRV+D STF P S+P +ML
Sbjct: 481 FETFCLDNVASYWHYHGGSLVGKVLDDSFRVMGIKALRVVDASTFPYEPNSHPQGFYLML 540
BLAST of Clc11G13630 vs. ExPASy TrEMBL
Match:
A0A0A0LJ76 (GMC_OxRdtase_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G049980 PE=3 SV=1)
HSP 1 Score: 864.8 bits (2233), Expect = 2.1e-247
Identity = 429/500 (85.80%), Postives = 462/500 (92.40%), Query Frame = 0
Query: 31 MKFVRDGSEFPEKEEYDYIIVGGGTAGCPLAATLSKNFSTLLIERGSEPNKYPYVLNEQK 90
MKFV+D SEFP KEEYDYIIVGGGT GCPLAATLSK FS LLIERGSEP+KYP VLNEQ+
Sbjct: 1 MKFVQDASEFPIKEEYDYIIVGGGTTGCPLAATLSKKFSVLLIERGSEPSKYPSVLNEQQ 60
Query: 91 LLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRANPEFFKTQLGVQDF 150
LLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRA+PEFF+TQLG+Q+
Sbjct: 61 LLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRADPEFFRTQLGMQEL 120
Query: 151 DMEMVEKAYQWVEEAIVYKPISLNPWQAAFRSFLVEGGIEPDNGFDLRDIVGTKISGSLF 210
DMEMVEKAY+WVEEAIV+KP SLN WQ AFR FLVEGG+EPDNGFDLRD VGTKISGS+F
Sbjct: 121 DMEMVEKAYEWVEEAIVFKP-SLNLWQGAFRRFLVEGGVEPDNGFDLRDDVGTKISGSIF 180
Query: 211 DENGTRHGAVQLLNKAKPTNLKVAVRATVQRILFSGLSANGVLYSDSKGKLHTTFIREKG 270
DENGTRHGAV+LLNKA+PTNLKVAV+A VQRILFSGLSANGVLYSDSKGKLHT +IR++G
Sbjct: 181 DENGTRHGAVELLNKAQPTNLKVAVQAIVQRILFSGLSANGVLYSDSKGKLHTAYIRKEG 240
Query: 271 EIILSGGALGSPQLLLLSGVGPKSYLSSLNLTVVLEQPHVGQGMIDNPRFGVNFAPQFPL 330
EII+S GA+GSPQLLLLSG+GPKSYLSSL L VVL QPHVGQ M+D PRFG A FPL
Sbjct: 241 EIIVSAGAIGSPQLLLLSGIGPKSYLSSLQLPVVLHQPHVGQSMMDYPRFGYVLAWPFPL 300
Query: 331 AFSSSKVIGISQNNSFYFQSIASTTPLSIPPLFSILPPNSTSLTTTSLATIGGKFSKIAS 390
+F+SSKVIGISQN +FYFQSIASTTPLSIPPLFSI PPNSTSLTTTSLATIGGKFSK+AS
Sbjct: 301 SFTSSKVIGISQNKTFYFQSIASTTPLSIPPLFSIFPPNSTSLTTTSLATIGGKFSKVAS 360
Query: 391 MGSLRLNSSTNVNKNPIVRFNYYSDPNDIAMCVRGVRKVGDFLQTQTIENIKTRDLEGNK 450
GSLRLNSS + NPIVRFNYYS P D+AMCV+GVRKVG FL+TQT+ENIKTRDLEGNK
Sbjct: 361 TGSLRLNSSADAASNPIVRFNYYSHPADVAMCVKGVRKVGGFLKTQTVENIKTRDLEGNK 420
Query: 451 RIQFVGLPLPENLSDDASVGEFCKKTVTSYWHYHGGCLVGKVVDGNYSVIGIKNLRVLDG 510
IQFVGLPLP NLSDD++VGEFCKKTVT YWHYHGGCLVGKVVDGNYSVIG+KNLRVLDG
Sbjct: 421 TIQFVGLPLPGNLSDDSAVGEFCKKTVTLYWHYHGGCLVGKVVDGNYSVIGVKNLRVLDG 480
Query: 511 STFAVSPGSNPTATLMMLAR 531
STFAVSPGSNPTATLMMLAR
Sbjct: 481 STFAVSPGSNPTATLMMLAR 499
BLAST of Clc11G13630 vs. ExPASy TrEMBL
Match:
A0A6J1EPL5 ((R)-mandelonitrile lyase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436223 PE=3 SV=1)
HSP 1 Score: 834.3 bits (2154), Expect = 3.0e-238
Identity = 417/543 (76.80%), Postives = 474/543 (87.29%), Query Frame = 0
Query: 1 MENSIMAAFLMLILVFLSHSQFGL--------NQDVRYMKFVRDGSEFPEKEEYDYIIVG 60
MENS M FL+L+L+FLSHSQ L NQD+ YMKFV DG++FPEKEEYDYIIVG
Sbjct: 1 MENSTMPTFLLLVLLFLSHSQLLLLISTNTRPNQDISYMKFVHDGNDFPEKEEYDYIIVG 60
Query: 61 GGTAGCPLAATLSKNFSTLLIERGSEPNKYPYVLNEQKLLNVFTVEDDGKNPFNRFISED 120
GGTAGCPLAATLSKNFSTLLIERGSEPNKYP VL EQ LLNVFTVEDDGKNP+NRF S++
Sbjct: 61 GGTAGCPLAATLSKNFSTLLIERGSEPNKYPSVLKEQALLNVFTVEDDGKNPYNRFTSKE 120
Query: 121 GVENIRGRVLGGSSMINGGVYSRANPEFFKTQLGVQDFDMEMVEKAYQWVEEAIVYKPIS 180
GV+N+RGRVLGG+SMINGGVYS+ N +FFKTQLGVQ FDMEMVEKAY+WVEEA+VY+P S
Sbjct: 121 GVQNLRGRVLGGTSMINGGVYSKPNTDFFKTQLGVQKFDMEMVEKAYKWVEEALVYRP-S 180
Query: 181 LNPWQAAFRSFLVEGGIEPDNGFDLRDIVGTKISGSLFDENGTRHGAVQLLNKAKPTNLK 240
LN WQ AFR LVEGGI PDNGF+LRD+VGTKI+GS+FDENG RHGAV+LLNKA+P NLK
Sbjct: 181 LNAWQNAFRRILVEGGIVPDNGFELRDVVGTKITGSIFDENGNRHGAVELLNKARPGNLK 240
Query: 241 VAVRATVQRILFSGLSANGVLYSDSKGKLHTTFIREKGEIILSGGALGSPQLLLLSGVGP 300
VA+RATV+RI+FSG SA+ VLYSDSKGKLHT + + GEIILS GA+GSPQLLLLSGVGP
Sbjct: 241 VAIRATVERIIFSGSSASEVLYSDSKGKLHTAVVHKNGEIILSAGAIGSPQLLLLSGVGP 300
Query: 301 KSYLSSLNLTVVLEQPHVGQGMIDNPRFGVNFAPQFPLAFSSSKVIGISQNNSFYFQSIA 360
KS+LSSLN+ ++L PHVG+ M DNPR GV FPL F+SSKVIGIS NN++YFQSIA
Sbjct: 301 KSHLSSLNIPLILHNPHVGEFMKDNPRVGVVPILPFPLDFTSSKVIGISNNNTYYFQSIA 360
Query: 361 STTPLSIPPLFSILPPNSTSLTTTSLATIGGKFSKIASMGSLRLNSSTNVNKNPIVRFNY 420
ST+P SIPP FS+LPPNSTS+ T +ATIGGKFSK+AS+GSLRLNSST+V NPIVRFNY
Sbjct: 361 STSPFSIPPSFSLLPPNSTSVNLT-VATIGGKFSKLASVGSLRLNSSTDVKTNPIVRFNY 420
Query: 421 YSDPNDIAMCVRGVRKVGDFLQTQTIENIKTRDLEGNKRIQFVGLPLPENLSDDASVGEF 480
YS+P DI +CV GVRKVGDFL+TQ I+NIKTRDLEGN+ IQF+G PLPENLSD +SV EF
Sbjct: 421 YSNPADIQLCVGGVRKVGDFLKTQIIQNIKTRDLEGNRVIQFLGPPLPENLSDYSSVAEF 480
Query: 481 CKKTVTSYWHYHGGCLVGKVVDGNYSVIGIKNLRVLDGSTFAVSPGSNPTATLMMLARKL 536
CK+TVT+YWHYHGGCLVGKVVDGNYSVIG+KNLRVLDGSTFAVSPGSNP ATLMML R +
Sbjct: 481 CKRTVTTYWHYHGGCLVGKVVDGNYSVIGLKNLRVLDGSTFAVSPGSNPIATLMMLGRYV 540
BLAST of Clc11G13630 vs. ExPASy TrEMBL
Match:
A0A6J1K9H9 ((R)-mandelonitrile lyase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492330 PE=3 SV=1)
HSP 1 Score: 832.0 bits (2148), Expect = 1.5e-237
Identity = 416/543 (76.61%), Postives = 472/543 (86.92%), Query Frame = 0
Query: 1 MENSIMAAFLMLILVFLSHSQFGL--------NQDVRYMKFVRDGSEFPEKEEYDYIIVG 60
MENS M FL+LIL+ LSHSQ L N+D+ YMKFVRDG++FPEKEEYDYIIVG
Sbjct: 1 MENSTMPTFLLLILLLLSHSQLRLLISTNTQPNEDISYMKFVRDGNDFPEKEEYDYIIVG 60
Query: 61 GGTAGCPLAATLSKNFSTLLIERGSEPNKYPYVLNEQKLLNVFTVEDDGKNPFNRFISED 120
GGTAGCPLAATLSKNFSTLLIERGSEPNKYP VL EQ LLNVFTVEDDGKNPFNRF+S++
Sbjct: 61 GGTAGCPLAATLSKNFSTLLIERGSEPNKYPSVLKEQALLNVFTVEDDGKNPFNRFVSKE 120
Query: 121 GVENIRGRVLGGSSMINGGVYSRANPEFFKTQLGVQDFDMEMVEKAYQWVEEAIVYKPIS 180
GV+N+RGRVLGG+SMINGGVYS+ N +FFKTQLGVQ FDMEMVEKAY+WVEEA+VY+P
Sbjct: 121 GVQNLRGRVLGGTSMINGGVYSKPNTDFFKTQLGVQKFDMEMVEKAYKWVEEALVYRP-R 180
Query: 181 LNPWQAAFRSFLVEGGIEPDNGFDLRDIVGTKISGSLFDENGTRHGAVQLLNKAKPTNLK 240
LN WQ AFR LVEGGI PDNGF+LRD+VGTKI+GS+FDENG RHGA +LLNKA+P NLK
Sbjct: 181 LNAWQNAFRRVLVEGGIVPDNGFELRDVVGTKIAGSIFDENGNRHGAEELLNKARPGNLK 240
Query: 241 VAVRATVQRILFSGLSANGVLYSDSKGKLHTTFIREKGEIILSGGALGSPQLLLLSGVGP 300
VA+RATV+RI+FSG SA GVLYSDSKGKLHT IR+ GEII+S GA+GSPQLLLLSGVGP
Sbjct: 241 VAIRATVERIIFSGSSATGVLYSDSKGKLHTAVIRKNGEIIVSAGAIGSPQLLLLSGVGP 300
Query: 301 KSYLSSLNLTVVLEQPHVGQGMIDNPRFGVNFAPQFPLAFSSSKVIGISQNNSFYFQSIA 360
KS+LSSLNL ++L PHVGQ M DNPR GV +PL F+SSKVIGIS NN++YFQSIA
Sbjct: 301 KSHLSSLNLPLILHNPHVGQFMKDNPRVGVIPILPYPLDFTSSKVIGISNNNTYYFQSIA 360
Query: 361 STTPLSIPPLFSILPPNSTSLTTTSLATIGGKFSKIASMGSLRLNSSTNVNKNPIVRFNY 420
+T+P SIPP FS+LPPNSTS+ T +ATIGGKFSK+ S+GSLRLNSST+V PIVRFNY
Sbjct: 361 TTSPFSIPPSFSLLPPNSTSVNLT-IATIGGKFSKLTSVGSLRLNSSTDVKTTPIVRFNY 420
Query: 421 YSDPNDIAMCVRGVRKVGDFLQTQTIENIKTRDLEGNKRIQFVGLPLPENLSDDASVGEF 480
YS+P DI +CV GVRKVGDFL+TQ I+NIKTRDLEGN+ IQF+G PLPENLSD +SV EF
Sbjct: 421 YSNPADIQLCVGGVRKVGDFLKTQIIQNIKTRDLEGNRVIQFLGPPLPENLSDYSSVAEF 480
Query: 481 CKKTVTSYWHYHGGCLVGKVVDGNYSVIGIKNLRVLDGSTFAVSPGSNPTATLMMLARKL 536
CK+TVT+YWHYHGGCLVGKVVDGNYSVIG+KNLRVLDGSTFAVSPGSNP ATLMML R +
Sbjct: 481 CKRTVTTYWHYHGGCLVGKVVDGNYSVIGLKNLRVLDGSTFAVSPGSNPIATLMMLGRYV 540
BLAST of Clc11G13630 vs. ExPASy TrEMBL
Match:
A0A6J1K7D5 ((R)-mandelonitrile lyase 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492330 PE=3 SV=1)
HSP 1 Score: 816.2 bits (2107), Expect = 8.5e-233
Identity = 413/542 (76.20%), Postives = 468/542 (86.35%), Query Frame = 0
Query: 1 MENSIMAAFLMLILVFLSHSQFGL-------NQDVRYMKFVRDGSEFPEKEEYDYIIVGG 60
ME S MA L LIL+ S QFG +QD+ YMKFVRDG++FPEKEEYDYIIVGG
Sbjct: 1 MERS-MATILFLILI--STFQFGAISSTTIPDQDISYMKFVRDGNDFPEKEEYDYIIVGG 60
Query: 61 GTAGCPLAATLSKNFSTLLIERGSEPNKYPYVLNEQKLLNVFTVEDDGKNPFNRFISEDG 120
GTAGCPLAATLSKNFSTLLIERGSEPNKYP VL EQ LLNVFTVEDDGKNPFNRF+S++G
Sbjct: 61 GTAGCPLAATLSKNFSTLLIERGSEPNKYPSVLKEQALLNVFTVEDDGKNPFNRFVSKEG 120
Query: 121 VENIRGRVLGGSSMINGGVYSRANPEFFKTQLGVQDFDMEMVEKAYQWVEEAIVYKPISL 180
V+N+RGRVLGG+SMINGGVYS+ N +FFKTQLGVQ FDMEMVEKAY+WVEEA+VY+P L
Sbjct: 121 VQNLRGRVLGGTSMINGGVYSKPNTDFFKTQLGVQKFDMEMVEKAYKWVEEALVYRP-RL 180
Query: 181 NPWQAAFRSFLVEGGIEPDNGFDLRDIVGTKISGSLFDENGTRHGAVQLLNKAKPTNLKV 240
N WQ AFR LVEGGI PDNGF+LRD+VGTKI+GS+FDENG RHGA +LLNKA+P NLKV
Sbjct: 181 NAWQNAFRRVLVEGGIVPDNGFELRDVVGTKIAGSIFDENGNRHGAEELLNKARPGNLKV 240
Query: 241 AVRATVQRILFSGLSANGVLYSDSKGKLHTTFIREKGEIILSGGALGSPQLLLLSGVGPK 300
A+RATV+RI+FSG SA GVLYSDSKGKLHT IR+ GEII+S GA+GSPQLLLLSGVGPK
Sbjct: 241 AIRATVERIIFSGSSATGVLYSDSKGKLHTAVIRKNGEIIVSAGAIGSPQLLLLSGVGPK 300
Query: 301 SYLSSLNLTVVLEQPHVGQGMIDNPRFGVNFAPQFPLAFSSSKVIGISQNNSFYFQSIAS 360
S+LSSLNL ++L PHVGQ M DNPR GV +PL F+SSKVIGIS NN++YFQSIA+
Sbjct: 301 SHLSSLNLPLILHNPHVGQFMKDNPRVGVIPILPYPLDFTSSKVIGISNNNTYYFQSIAT 360
Query: 361 TTPLSIPPLFSILPPNSTSLTTTSLATIGGKFSKIASMGSLRLNSSTNVNKNPIVRFNYY 420
T+P SIPP FS+LPPNSTS+ T +ATIGGKFSK+ S+GSLRLNSST+V PIVRFNYY
Sbjct: 361 TSPFSIPPSFSLLPPNSTSVNLT-IATIGGKFSKLTSVGSLRLNSSTDVKTTPIVRFNYY 420
Query: 421 SDPNDIAMCVRGVRKVGDFLQTQTIENIKTRDLEGNKRIQFVGLPLPENLSDDASVGEFC 480
S+P DI +CV GVRKVGDFL+TQ I+NIKTRDLEGN+ IQF+G PLPENLSD +SV EFC
Sbjct: 421 SNPADIQLCVGGVRKVGDFLKTQIIQNIKTRDLEGNRVIQFLGPPLPENLSDYSSVAEFC 480
Query: 481 KKTVTSYWHYHGGCLVGKVVDGNYSVIGIKNLRVLDGSTFAVSPGSNPTATLMMLARKLK 536
K+TVT+YWHYHGGCLVGKVVDGNYSVIG+KNLRVLDGSTFAVSPGSNP ATLMML R +
Sbjct: 481 KRTVTTYWHYHGGCLVGKVVDGNYSVIGLKNLRVLDGSTFAVSPGSNPIATLMMLGRYVG 537
BLAST of Clc11G13630 vs. ExPASy TrEMBL
Match:
A0A0A0LK76 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055000 PE=3 SV=1)
HSP 1 Score: 718.4 bits (1853), Expect = 2.4e-203
Identity = 362/535 (67.66%), Postives = 432/535 (80.75%), Query Frame = 0
Query: 1 MENSIMAAFLMLILVFL----SHSQFGL-NQDVRYMKFVRDGSEFPEKEEYDYIIVGGGT 60
ME+S + ++ +++ + HS + NQDV YMKFV + ++ P KEEYDYII+GGGT
Sbjct: 1 MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGT 60
Query: 61 AGCPLAATLSKNFSTLLIERGSEPNKYPYVLNEQKLLNVFTVEDDGKNPFNRFISEDGVE 120
AGCPLA TLS FS LL+ERGS+PNKYP VL+EQ LLNVF DDG+NPF RF+SEDGVE
Sbjct: 61 AGCPLATTLSSKFSVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVE 120
Query: 121 NIRGRVLGGSSMINGGVYSRANPEFFKTQLGVQDFDMEMVEKAYQWVEEAIVYKPISLNP 180
NIRGRVLGG SM+N G YSR + EFF GV D+DME+VEKAY+WVEE +V +PI LN
Sbjct: 121 NIRGRVLGGGSMVNAGFYSRGHREFF-ASAGV-DWDMELVEKAYEWVEETVVSQPI-LNA 180
Query: 181 WQAAFRSFLVEGGIEPDNGFDLRDIVGTKISGSLFDENGTRHGAVQLLNKAKPTNLKVAV 240
WQ+AFRS L+EGG+ PD+GFDLR +VGTK GS+FD G RHGAV+LLNKA PTN+KVA+
Sbjct: 181 WQSAFRSSLLEGGVVPDSGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAI 240
Query: 241 RATVQRILFSGLSANGVLYSDSKGKLHTTFIREKGEIILSGGALGSPQLLLLSGVGPKSY 300
ATVQRILFSGLSANGVLYSDSKGKLH IR+KGEII+S GA+GSPQLLLLSG+GPKS+
Sbjct: 241 EATVQRILFSGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSH 300
Query: 301 LSSLNLTVVLEQPHVGQGMIDNPRFGVNFAPQFPLAFSSSKVIGISQNNSFYFQSIASTT 360
LSSL L VVL QP+VGQ M DNPRFG N FP+ SS KV+GI Q+N Y QSIAS
Sbjct: 301 LSSLKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDN-IYIQSIASPF 360
Query: 361 PLSIPPLFSILPPNSTSLTTTSLATIGGKFSKIASMGSLRLNSSTNVNKNPIVRFNYYSD 420
P+ IP +FS+LPP +TS+ T LA GKFS++ S GSLRLNSSTNV K+PIV FNYYS
Sbjct: 361 PILIPQIFSLLPPQATSIIPT-LAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSH 420
Query: 421 PNDIAMCVRGVRKVGDFLQTQTIENIKTRDLEGNKRIQFVGLPLPENLSDDASVGEFCKK 480
P+D+ CV+GVRK+GD L+T+T+E IKT++LEGNK +F+G+PLPENL +D+SV E+CKK
Sbjct: 421 PDDLGRCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKK 480
Query: 481 TVTSYWHYHGGCLVGKVVDGNYSVIGIKNLRVLDGSTFAVSPGSNPTATLMMLAR 531
TVT+YWHYHGGCLVGKVVDGNY VIGIKNLRV+DGSTF+ SPG+NP ATLMML R
Sbjct: 481 TVTTYWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 530
BLAST of Clc11G13630 vs. TAIR 10
Match:
AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )
HSP 1 Score: 485.3 bits (1248), Expect = 6.6e-137
Identity = 251/538 (46.65%), Postives = 358/538 (66.54%), Query Frame = 0
Query: 6 MAAFLMLILVFLSHSQFGLNQDVRYMKFVRDGSEFPEKEEYDYIIVGGGTAGCPLAATLS 65
+ L+L +V S+++ +N+ +M+F+ + ++F ++ YDYIIVGGGTAGCPLAATLS
Sbjct: 14 LVVLLLLGVVHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLS 73
Query: 66 KNFSTLLIERGSEPNKYPYVLNEQKLLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGS 125
++F LL+ERG P P V++ L T ++ +P FISE+GV N RGRVLGGS
Sbjct: 74 QSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGS 133
Query: 126 SMINGGVYSRANPEFFKTQLGVQDFDMEMVEKAYQWVEEAIVYKPISLNPWQAAFRSFLV 185
S IN G YSRA+ +FF+ V +D+ V ++Y+WVE AIV++P L WQ A R L+
Sbjct: 134 SAINAGFYSRADKQFFENSGLV--WDLSSVNQSYEWVERAIVFRP-QLRTWQTAIRDALL 193
Query: 186 EGGIEPDNGFDLRDIVGTKISGSLFDENGTRHGAVQLLNKAKPTNLKVAVRATVQRILF- 245
E G+ P NGF L VGTKI GS FD G RH + LL A+ +N++VAV ATV+R+L
Sbjct: 194 EVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVERVLLA 253
Query: 246 -------SGLSANGVLYSDSKGKLHTTFIREKGEIILSGGALGSPQLLLLSGVGPKSYLS 305
S +SA GV+Y D G+ H IR++GE+ILS GALGSPQLL LSG+GP+SYLS
Sbjct: 254 SSPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLS 313
Query: 306 SLNLTVVLEQPHVGQGMIDNPRFGVNFAPQFPLAFSSSKVIGISQNNSFYFQSIASTTPL 365
+ + V L+QPHVG + DNPR G++ P P+ S +V+G++++ +F ++ ++ P
Sbjct: 314 TWGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAF-LEAASNVIPF 373
Query: 366 SIPPLFSILPPNSTSLTTTSLATIGGKFSKIASMGSLRLNSSTNVNKNPIVRFNYYSDPN 425
+ PL S+ S + TI K S+G LRL +ST+V NP+VRFNY+SDP
Sbjct: 374 A-SPLHSVFIRAPASPLYVPVTTIMEKILGPVSIGLLRL-ASTDVRINPVVRFNYFSDPQ 433
Query: 426 DIAMCVRGVRKVGDFLQTQTIENIKTRDLEGNKRIQFVGLPLPENLSDDASVGEFCKKTV 485
D+ CV G RK+G+ L+++ +++ R+ GN+R +FVG PLP + S+D + +FC++TV
Sbjct: 434 DLERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMADFCRRTV 493
Query: 486 TSYWHYHGGCLVGKVVDGNYSVIGIKNLRVLDGSTFAVSPGSNPTATLMMLARKLKLK 536
++ WHYHGG +VGKVVD + VIG+ +LR++DGSTF +SPG+NP ATLMML R + LK
Sbjct: 494 STIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLGRYMGLK 545
BLAST of Clc11G13630 vs. TAIR 10
Match:
AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )
HSP 1 Score: 378.6 bits (971), Expect = 8.7e-105
Identity = 226/562 (40.21%), Postives = 304/562 (54.09%), Query Frame = 0
Query: 10 LMLILVFLSHSQFGLNQDVRYMKFVRDGSEFPEKEEYDYIIVGGGTAGCPLAATLSKNFS 69
L + L HS + F+RD + P YDYII+GGGTAGCPLAATLS+N S
Sbjct: 10 LFVALSLFLHSPICSSDKAPNYSFMRDATGSPTTSYYDYIIIGGGTAGCPLAATLSQNAS 69
Query: 70 TLLIERGSEPNKYPYVLNEQKLLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMIN 129
LL+ERG P P + + +P RF+SEDGV N R RVLGG S +N
Sbjct: 70 VLLLERGDSPYNNPNI-TRLSAFGAALSDLSESSPSQRFVSEDGVINARARVLGGGSALN 129
Query: 130 GGVYSRANPEFFKTQLGVQDFDMEMVEKAYQWVEEAIVYKPISLNPWQAAFRSFLVEGGI 189
G Y+RA ++ + +D + ++YQWVE + ++P + WQ A R L+E GI
Sbjct: 130 AGFYTRAGTKYVRN----MGWDGALANESYQWVEAKVAFQP-PMGRWQTAVRDGLLEAGI 189
Query: 190 EPDNGFDLRDIVGTKISGSLFDENGTRHGAVQLLNKAKPTNLKVAVRATVQRILFSGLS- 249
P+NGF I GTK G++FD NG RH A LL A P + V + ATV RILF
Sbjct: 190 VPNNGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRILFRTRGT 249
Query: 250 ----ANGVLYSDSKGKLHTTFIRE--KGEIILSGGALGSPQLLLLSGVGPKSYLSSLNLT 309
ANGV+Y D G+ H +++E EIILS G LGSPQLL+LSGVGP + L + N+T
Sbjct: 250 TKPIANGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNIT 309
Query: 310 VVLEQPHVGQGMIDNPRFGVNFAPQFPLAFSSSKVIGISQNNSFYFQS---------IAS 369
VV++QPHVGQGM DNP V P+ S +V+GI+ ++ + S
Sbjct: 310 VVMDQPHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGGGGGGS 369
Query: 370 TTPLSIPPLFSILPPNSTSLTTTSLATI-------GG----KFSKIASMGSLRLNSSTNV 429
+ S +++ P +T L + S+ + GG K S G L L + N
Sbjct: 370 SGSSSTRDYYAMFSPRATLLESNSMTKLSSAQPFQGGFLLEKVMGPLSTGHLEL-KTRNP 429
Query: 430 NKNPIVRFNYYSDPNDIAMCVRGVRKVGDFLQTQTIENIKTRDLEGNKRIQFVGLPLPEN 489
NPIV FNY+ P+D+ CVRG++ + +Q++ K D+ + P N
Sbjct: 430 KDNPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADVSFEYLLNLTA-STPVN 489
Query: 490 L---------SDDASVGEFCKKTVTSYWHYHGGCLVGKVVDGNYSVIGIKNLRVLDGSTF 536
L S S EFC+ TVT+ WHYHGGC+VG+VVDG+Y VIGI LRV+D ST
Sbjct: 490 LRPPRSGPGASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRLRVIDMSTV 549
BLAST of Clc11G13630 vs. TAIR 10
Match:
AT5G51930.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )
HSP 1 Score: 362.1 bits (928), Expect = 8.5e-100
Identity = 228/556 (41.01%), Postives = 324/556 (58.27%), Query Frame = 0
Query: 12 LILVFLSHSQFGLNQDVRYMKFVRDGSEFPEKEEYDYIIVGGGTAGCPLAATLSKNFSTL 71
LILV++S F L + F++D + P+ +DYII+GGGTAGC LAATLS+N S L
Sbjct: 41 LILVYISIFFFFLVK--LETTFMKDATLAPKNASFDYIIIGGGTAGCALAATLSQNASVL 100
Query: 72 LIERGSEPNKYPYVLNEQKLLNVFTVEDDGKNPFNR-FISEDGVENIRGRVLGGSSMING 131
++ERG P + P + +N T+ ++ N +++ FISEDGV N R RVLGG S+ING
Sbjct: 101 VLERGGSPYENPTATDMGNSVN--TLLNNTPNSWSQLFISEDGVYNTRPRVLGGGSVING 160
Query: 132 GVYSRANPEFFKTQLGVQDFDMEMVEKAYQWVEEAIVYKPISLNPWQAAFRSFLVEGGIE 191
G YSRA ++ + +++ME VE AY+WVE+ +V++P + WQ AF+ L+E G
Sbjct: 161 GFYSRAGNDYVEE----AEWEMEEVEAAYEWVEKKLVFEP-QVIEWQKAFKDGLLEAGES 220
Query: 192 PDNGFDLRDIVGTKISGSLFDENGTRHGAVQLLNKAKPTNLKVAVRATVQRILFSGLSAN 251
PDNGF I GTKI G++FD G RH A LL A P + V + A+V ++LF+ A
Sbjct: 221 PDNGFTYDHIYGTKIGGTIFDRAGHRHTAANLLEYANPNRIVVYLHASVHKVLFT-TEAY 280
Query: 252 GVLYSDSKGKLHTTFIREK--GEIILSGGALGSPQLLLLSGVGPKSYLSSLNLT-VVLEQ 311
VL+ D+ G H + K E+ILS GALGSPQLL+LSGVGP +L + + +VL+Q
Sbjct: 281 EVLFEDANGVFHKANLANKATNEVILSAGALGSPQLLMLSGVGPAVHLEAHGVNPLVLDQ 340
Query: 312 PHVGQGMIDNPRFGVNFAPQFPLAFSSSKVIGISQNNSFYFQSIA------STTPLSIPP 371
P VGQGM DNP V P+ S + +GI++ +S Y + ++ T
Sbjct: 341 PMVGQGMADNPMNFVAIPSPQPVELSLIQAVGITKFDS-YIEGLSGLSLSFDITRRFFDG 400
Query: 372 LFSILPPNSTSLTTT------SLATI--------------GGKFSKI---ASMGSLRLNS 431
+ ++L N TS TT+ S+A + G F K+ AS G ++L
Sbjct: 401 VLNLL--NETSHTTSRKILTQSIAVLLKSFDVKLEVRMNGGLIFQKVDGPASKGHMKLR- 460
Query: 432 STNVNKNPIVRFNYYSDPNDIAMCVRGVRKVGDFLQTQTIENIKTRDLEGNKRIQ-FVGL 491
+TN NP V FNYY +P D+ CV+G+ + + ++ K + + + + L
Sbjct: 461 NTNPRDNPSVTFNYYQEPEDLNKCVKGLNTIIRMINSKAFSKYKYPGVTARELLNLMLAL 520
Query: 492 PL---PENLSDDASVGEFCKKTVTSYWHYHGGCLVGKVVDGNYSVIGIKNLRVLDGSTFA 531
P+ P +++ ++ +FC TVTS WHYHGGC VGKVVD NY V+GI LRV+DGSTF
Sbjct: 521 PINLRPRHVTSAFNLKQFCIDTVTSVWHYHGGCQVGKVVDKNYKVLGIDGLRVIDGSTFL 580
BLAST of Clc11G13630 vs. TAIR 10
Match:
AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )
HSP 1 Score: 355.5 bits (911), Expect = 7.9e-98
Identity = 217/531 (40.87%), Postives = 297/531 (55.93%), Query Frame = 0
Query: 42 EKEEYDYIIVGGGTAGCPLAATLSKNFSTLLIERGSEPNKYPYVLNEQKLLNVFTVEDDG 101
+ YDYI++GGGTAGCPLAATLS+NFS L++ERG P V + + ++ +
Sbjct: 59 QDSSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANV-SFLRNFHIGLADISA 118
Query: 102 KNPFNRFISEDGVENIRGRVLGGSSMINGGVYSRANPEFFKTQLGVQDFDMEMVEKAYQW 161
+ F+S DGV N R RVLGG S IN G YSRA+ F K +D ++V+++Y W
Sbjct: 119 SSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKR----AGWDPKLVKESYPW 178
Query: 162 VEEAIVYKPISLNPWQAAFRSFLVEGGIEPDNGFDLRDIVGTKISGSLFDENGTRHGAVQ 221
VE IV++P L WQ A R L+E G+ P NGF + GTKI G++FD G RH A +
Sbjct: 179 VEREIVHQP-KLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAE 238
Query: 222 LLNKAKPTNLKVAVRATVQRILF--SGL--SANGVLYSDSKGKLHTTFI--REKGEIILS 281
LL A P L+V + ATVQ+I+F SG GV++ D KG H + R+ E+ILS
Sbjct: 239 LLAYANPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILS 298
Query: 282 GGALGSPQLLLLSGVGPKSYLSSLNLTVVLEQPHVGQGMIDNPRFGVNFAPQFPLAFSSS 341
GA+GSPQ+L+LSG+GPK L L + VVLE HVG+GM DNP + + P+ S
Sbjct: 299 SGAIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLI 358
Query: 342 KVIGISQNNSFYFQSIA-STTPLSI----------PPLFSILP-----PNSTSLTTTS-- 401
+ +GI++ + S +P SI LFS +P P +T T
Sbjct: 359 QTVGITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNK 418
Query: 402 ---LATIGGKF--SKIA---SMGSLRLNSSTNVNKNPIVRFNYYSDPNDIAMCVRGVRKV 461
G F K+A S G L L +TNV+ NP V FNY+ P D+ CV +R V
Sbjct: 419 YQLHEAFNGSFILEKLAYPISRGHLSL-VNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLV 478
Query: 462 GDFLQTQTIENIKTRDLEGNKRIQFVGLPL-----PENLSDDASVGEFCKKTVTSYWHYH 521
+ + N D + ++ + + P+ L+D S+ +FCK TV + WHYH
Sbjct: 479 SKVVTSNRFLNYTQCDKQNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWHYH 538
Query: 522 GGCLVGKVVDGNYSVIGIKNLRVLDGSTFAVSPGSNPTATLMMLARKLKLK 536
GGCLVGKVV N V+G+ LRV+DGSTF SPG+NP AT+MM+ R + +K
Sbjct: 539 GGCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVK 582
BLAST of Clc11G13630 vs. TAIR 10
Match:
AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )
HSP 1 Score: 346.7 bits (888), Expect = 3.7e-95
Identity = 214/564 (37.94%), Postives = 309/564 (54.79%), Query Frame = 0
Query: 11 MLILVFLSHSQFGLNQDVRYMKFVRDGSEFPEKEEYDYIIVGGGTAGCPLAATLSKNFST 70
+ I+ ++ H+ ++ Y F++D + P +DYII+GGGT+GC LAATLS+N S
Sbjct: 12 IFIITYIFHASCSPDK-AGYYSFMKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASV 71
Query: 71 LLIERGSEPNKYPYVLNEQKLLNVFTVEDDGKNPFNRFISEDGVENIRGRVLGGSSMING 130
L++ERG P P + + + K+ FISEDGV N R RVLGG S++N
Sbjct: 72 LVLERGGAPYDNPTATDIENFATTLS-NTSPKSWSQLFISEDGVYNTRARVLGGGSVLNA 131
Query: 131 GVYSRANPEFFKTQLGVQDFDMEMVEKAYQWVEEAIVYKPISLNPWQAAFRSFLVEGGIE 190
G Y+RA E+ K ++ + VE AY+WVE+ + ++P L WQ AF+ L+E G
Sbjct: 132 GFYTRAGDEYVKE----TEWKTDEVEAAYEWVEKKVAFQPPVLG-WQTAFKDGLLEAGEF 191
Query: 191 PDNGFDLRDIVGTKISGSLFDENGTRHGAVQLLNKAKPTNLKVAVRATVQRILFSGL--- 250
P NGF I GTKI G++FD G RH A LL A P N+ V + A+V +ILF+
Sbjct: 192 PYNGFTYDHIYGTKIGGTIFDRAGHRHTAADLLEYANPGNIVVYLHASVHKILFTTKGRP 251
Query: 251 --SANGVLYSDSKGKLHTTFIREK--GEIILSGGALGSPQLLLLSGVGPKSYLSSLNL-T 310
A GV++ D+ G LH + + E+ILS GA+GSPQLL+LSG+GP ++L++ +
Sbjct: 252 RPKAYGVIFQDANGVLHKAELEKNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKP 311
Query: 311 VVLEQPHVGQGMIDNPRFGVNFAPQFPLAFSSSKVIGISQNNSF---------------- 370
+VL+ P VGQGM DNP + P+ S +V+GI++ S+
Sbjct: 312 LVLDHPMVGQGMGDNPMNAIFIPSPTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRR 371
Query: 371 YFQSI---------ASTTPLSIPPLFS------ILPPNSTSLTTTSLATIGGKFSKIASM 430
+F + + TT + P L + P N TT I K + S
Sbjct: 372 FFDGVLNYLNEMQTSRTTSTTSPTLSTQSITDFFNPINPLLNATTRAGLILQKIAGPISR 431
Query: 431 GSLRLNSSTNVNKNPIVRFNYYSDPNDIAMCVRGVRKVGDFLQTQTIENIKTRDLEGNKR 490
G L L +TN + NP VRFNYY +P D+ +CV G+ + + ++ K D +
Sbjct: 432 GHLELR-NTNPDDNPSVRFNYYQEPEDLQICVEGINTIIKVINSKAFSKFKYPDATIHGL 491
Query: 491 IQFVGLPLPENL-----SDDASVGEFCKKTVTSYWHYHGGCLVGKVVDGNYSVIGIKNLR 531
+ + L +P NL + ++ +FC TV + WHYHGGC VG+VVD NY V+GI +LR
Sbjct: 492 LDLM-LSVPTNLRPRHITSMFNLRQFCIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSLR 551
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
P52706 | 7.1e-144 | 48.17 | (R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1 | [more] |
P52707 | 3.9e-142 | 46.77 | (R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1 | [more] |
Q945K2 | 8.7e-142 | 47.62 | (R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1 | [more] |
O82784 | 6.9e-139 | 47.81 | (R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1 | [more] |
O24243 | 8.4e-137 | 47.17 | (R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A0A0LJ76 | 2.1e-247 | 85.80 | GMC_OxRdtase_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G049... | [more] |
A0A6J1EPL5 | 3.0e-238 | 76.80 | (R)-mandelonitrile lyase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436223 PE... | [more] |
A0A6J1K9H9 | 1.5e-237 | 76.61 | (R)-mandelonitrile lyase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111... | [more] |
A0A6J1K7D5 | 8.5e-233 | 76.20 | (R)-mandelonitrile lyase 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111... | [more] |
A0A0A0LK76 | 2.4e-203 | 67.66 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055000 PE=3 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
AT1G73050.1 | 6.6e-137 | 46.65 | Glucose-methanol-choline (GMC) oxidoreductase family protein | [more] |
AT1G12570.1 | 8.7e-105 | 40.21 | Glucose-methanol-choline (GMC) oxidoreductase family protein | [more] |
AT5G51930.1 | 8.5e-100 | 41.01 | Glucose-methanol-choline (GMC) oxidoreductase family protein | [more] |
AT1G72970.1 | 7.9e-98 | 40.87 | Glucose-methanol-choline (GMC) oxidoreductase family protein | [more] |
AT5G51950.1 | 3.7e-95 | 37.94 | Glucose-methanol-choline (GMC) oxidoreductase family protein | [more] |