Clc11G13550 (gene) Watermelon (cordophanus) v2

Overview
NameClc11G13550
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
Description(R)-mandelonitrile lyase 1-like
LocationClcChr11: 24208022 .. 24213243 (+)
RNA-Seq ExpressionClc11G13550
SyntenyClc11G13550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGAAAAGAAAAACAAAGTATGGAGAATTTTATGGCTACTATTCTCCTCTTCATCCTCATCTCTATCTTTCAATTAGGATTTCTCTCCTCCAACACTATCCCCAATCAAGGTAAGAACCCTCCCTCTAATACTTTTTTTTTTTTTTTCTTCTCCTTCTTTTTAAGCTTCTTTCTCTATTTATTATTGTTTTCAAGTATAAAAAAATGAACCAAAATATTTACAAATATAGCAAAATTTTACTGTTTATATGCAATAGACCGCGATAAACTATTATCTATATTTATCTGTGTCATGATAGACATGGATAGTAGTCTATCAGGATCTATCATAGATAGATAATGATATTTTACTATTATTTATAAATATTTTCAGCAGTTTTATCATTTAAAATAATTTTTCATGTTGATGTAATATTAAATTTATTATAACTCAAAAGTTTAAGGTTTGGGTTTTGTTGGTGATTTAACATGTATCAAAGTAAGAGTAAGAATTTTGAGTATGTTTCCTCCCTAACTAATATTGAATTTTACTTGATGGGCGTTTTTTGAGTCCACAGATGAGGAAGATTTTTAACAATTCTTATTGTGTTTGAAACTTTTCTTTTGCAGATACTAGTTACATGAACTTTGTACATGAGGCTAGTGAGTTACCAGAAAAAGAAGAACATGACTACATAGTAATAGGAGGAGGAACTGCAGGCTGTCCATTAGCTGCGACATTATCATCGAAATTTTCAGTTCTCCTTCTTGAAAGAGGCAGTGATCCTAATAAATATCCTTCTGTGCTAAATGAACAAGGCCTATTGAATGCTTTTGTTGCAGAAGATGATGGCCAAAATCCCTTCCAACATTTCACTTCTGAGGATGGTGTAGAGAACCTAAGAGGGCGTGTTCTCGGTGGTGGTAGCATGATCAATGCTGGCTTCTACTCGAGGGGTCATCGTGAGTTCTTTGAAACTGCAGGTGTTGATTGGGACATGGAATTGGTGGAAAAAGCTTATCAATGGGTTGAAGAAACTGTGGTCTCTAGGCCAAGTTTGAGTGCTTGGCAATCAGCTTTTAGGAGTGCATTGTTGGAAGCTGGGGTTGGTCCTGATAATGGGTTTGATTTGAGGCACCTTGTTGGGACTAAAACTGGAGGGTCCATCTTTGATGACAAAGGAAATAGACATGGAGCTGTAGAACTTCTCAACAAAGCTGAACCCAAAAACCTTAAAGTTGCAACTCAGGCCACAGTCCAAAGAATCATCTTCGATGGTTAGTTCCTAGCAAATCTTTGGTTTAAATTTCGTGTTGCATAATAGTTTTACGAAAGATAACATTATTGTTTTTACTATTCTAAATTCATAGGTTTATCGGCAAGTGGGGTTTCATATTTTGATTCAAAAGGAAAGTTGCATACAGCATTCATCCACAAGAAAGGAGAGATAATTGTAAGTGCAGGAGCCATTGGAAGCCCTCAACTTCTCCTTCTAAGTGGGGTTGGCCCAAAATCTCATCTTTTGTCTTTAAGACTACCTGTCGTCCTCGACCAACCACATGTTGGAGGATTCATGTCGGACAATCCCCGTTTGAGTCCCACCCTTGTACTTCCATTCCAACTAGTTGCTTCATCTGCACAAATCGTTGGAACATTAGACAACAATATTCACTTGCAATCATTTGCTAGCCCTTTACCATTTTTTGCTCCGCCATCTTTTAGTCTTCTTCCTCCTCAATCCACTTCCATCATCCCTAGCTTAGCCATTTTTGTTGGAAAATTCTCTGATGTCCATTCTGAGGGGTCACTCCGATTGAATTCCTCGACTGATGTGAAGAATAGTCCTATTGTTCGATTCAATTATTATTCACATCCTGATGATCTTGCTCGATGTGTTAGAGGAGTAAGAAAAGTGGGAGATTTGCTTACAACGCAAACCATGGAAAAGATGAAGACCCAAGACATAGAGGGTAATAAAGGATTTCAGTTTCTAGGGCTTCCCTTGCCAGAAAATTTGTGGAATGATAGCTCTGTCGAAGAATACTGTCAGAAGACGGTGACAACTTATTGGCATTATCATGGAGGATGTTTGGTCGGAAAAGTGGTCGATGATAACCACAAAGTCATTGGAATTGAAAATTTACGTGTAGTGGATGGCTCCACTTTCTCTGTCTCACCAGGAACTAATCCTATGGCCACCCTGATGATGCTCGGCCGGTGAGTGATATTACACACATGCATATTACATACACACACATATATAGCTTTATTCAAGAATGATTTTCTTGTCTCCACATCAATTTTCACTCCTTTTTTTTTTCTTTGTTTGGTTATATTATAAATATAGTCCATCAGCTTTTGTTTTTTATTTTTTAGAACTGTCTAATAAGTCTTGTTCTTGATTTTTTTTTTCTTTTAAATTTATGAACAAAATTTAATTAGACATAAAATTAAGATAGCATGAAGTTTTAAGAAAGCGTATATGATACGTGAAATAGTAGTTTTAAGACTTTTGGACGAAAATTCCATGCATGCTTTATTTGACCCAACTTATTTCTCAAGATTTTTGGAACTGAATTTTAAAACATTTCAAAGCTTTCTTAAACATAGAAGTAATTTTTAGGGAAAAAAAAAGCACTAAAAAAAAGTTTGGAGATTTTTTTTTAATAAGTAACAATAGCATAAAGATTTTTTATAGAAACAAAAATATATATATACTACAGTTAATGTATTAGTAGATACTTGAGGTTTTTTACCATTATCTTTTATTTCCATAAACTAAAAATTTCAGTAGGTATTTGAGTTTTTTTTTTTTTTTTTTCTAATTATCTTTTATTTCCATAAAATAAAATTTTCAAAAATATATTTTGCAGGTATGTTGGCCTCAAGGTACTGCAACAAAGATCAAGTTAGAGTGTGCAAACTAAGACACAGACTATTTTCATTTTGTGTAGCATTCTTGGTTGTGGGGGATGTATCTTGTCTTTAATTATTATTATTATTATTCTTCTTTGAAATGAAAATGTTCTCTTTTCTGAAGTTCAACAAAGTTGGGTTGATAATTGTTCTTTATTTTCTTGTGTCTTGTTGTTAGAATTATTAAATTATTTTTATTTGTCGTCTTTCATATTTTCTCGGTTTCTAAATTTATAGACAAAATTTAGAAAAAAAGTATATTGTTTTTCTTATTTCCATATTATGTCTAGTTTTTCAAGAAAATAACTATATTTATTATTTTATCATTTTTACTCATAAAATAGACGGAAGAACAAAAACTAAAACTCATCATTGTTGTTAATTAATTAATTATAATTATTATGACAAAAACTATTACCAGAACTCATTAACAGTCTTGTGGCCAGCAAAAGAATTTAATGGCTTTAAGCTACAAATAGTGAATAATCAATGATCCAGCTAATCACTGATATAATATAAATGTTATTAAATTACGTATTAATAAATCATTGACTCATTATGAGTAGGTTTCGTAAATAACTAATAAAATAAATGAAAATTTGATTAAAAAATAGGAAAAAAAGTGATTAAATAAACTAAGAAAGTACAAAACGAAAAAGAAAAAATATCATAATTTCAAATTCTCACCTCCAAATTTCTTGTGATTGAGAAAAAAATAAGAAAAAATATAGCTTTGAGAATAATAAGGAAAATGAAAACAATCTTTGTTAGATCTGATTATGCAATTGGCAATATTTGTTTAAGGTTGTGGATTATAATTCATCATGTTATCTTTGCATCTACTATTTTTGTCTAATTGTTATTTTTGAAAGGGTTGAATCTGCAAAATATATTTTCTTTATTTGAAAAATTCCTGCAGTCTTTTGTAAATTTCTTGCCTTTTCTAAATGCAACTATTTGGTAATTAGAGTTGTTCTCTTGGTTTTCCTTTGTGTATATTTAAGGAATTGTGGTTGGTCTTCTGAGGTGAAAATTATTTTGTGCCATTGTCTTGTGTTTGTGTTAGAAACACAAGTGTGTCAATTCATGTGTTTGTGAAGCTTTTTCTATTGTAGATCTTGAGTGTTTCTCTAGATCTTGTTGAATCCTTCTATTACAACTTGAGAAGGGATTCTCAATTTTAGGGGAGCCTAAGATTTCCAAGTGAAGTGGAAAGATAAAAGTCTTAGTGAAGAGGGAGTCTCACTCCTAAGGGAGCCTAAGTTATCTCGAGAAGGAATCTCACACTTAGGGGAAGCCAAAGTGATCATTTACTAATACGCACAATCTTAGGGGAAACTGCCACCCAAGACTTGTTAAATGATTTTCGTTCCTACTTAGCTTGAGATAGCATATTTGTCAATTAAAATACAAATTGTGCTTAAGTAACCAAGGGGTTTGAAATTGCAAGAAATTTAAAGAGCGTGAAAGTAAAGTTGCAATGGAATTAAATGTGGTAGTGTGAGAGTGAAATTAGAAGTAGTCCTTATGGAATTAATGGGGCTAGTTAGGGATGTTTCGGAGTTCCTTTTTAACTAATAATCTCTTGACCATTTTGCCCATATGTATGATCAATACTTGTATCTATAACTTAGTTGATTAATTAGAAATGCTAACTAAAAGTCCCAATTGATCAATTAAACTATGATGAAATTATGAACGCATAAGAATCACTTAGCGCTAACGCATTATGTTCAAGAGGGAAACTGCTAAGATGAACAATAATAAAGAACGCCTAATTAAATGCTCAAGGCTGGTTTCTTACGTGGTTGGTTGATCAATATTCATTTAAAGGATGCCCAAAAACTTACAAGTAAACCTAACCAATTGTTACTTGGTGAATAACTACTTAACAATTATGGATTAAGAGTGAGTCTATAGGAATCAACTATGTAAAATCGTGACTAGTGTCTCAAGAAATCTCGATAAACATAATCATTAGGTTCATTACAGTAGAAAAGCCCATCAATACAAGAAAAATTTAGTCAAGATGAAGAAAAAGTGATACATAGAAAATAAACTCTTAATTCTCAAGCTAGAACTTATAAATTTAGCCGATAATCATCCTTCAAACATAATCCCAATAATGAAATTGCATAAGAATTAATGGTAAAGAAATATGGGGAATAGTGTAGAGTTCAATTTTTGCTTAGAGAGAGTAGGAAAATTGGATGCTGCAAACGTTTGACGGCTAGGTCACTTAAGTCAAATTTCGAACTAGCATCGCGGTGCTCCTTGTCTGCAAGCTCACACGCACATTACACAATGATCTATCAAAATTCGTAG

mRNA sequence

AAAGAAAAGAAAAACAAAGTATGGAGAATTTTATGGCTACTATTCTCCTCTTCATCCTCATCTCTATCTTTCAATTAGGATTTCTCTCCTCCAACACTATCCCCAATCAAGAAAAAGAAGAACATGACTACATAGTAATAGGAGGAGGAACTGCAGGCTGTCCATTAGCTGCGACATTATCATCGAAATTTTCAGTTCTCCTTCTTGAAAGAGGCAGTGATCCTAATAAATATCCTTCTGTGCTAAATGAACAAGGCCTATTGAATGCTTTTGTTGCAGAAGATGATGGCCAAAATCCCTTCCAACATTTCACTTCTGAGGATGGTGTAGAGAACCTAAGAGGGCGTGTTCTCGGTGGTGGTAGCATGATCAATGCTGGCTTCTACTCGAGGGGTCATCGTGAGTTCTTTGAAACTGCAGGTGTTGATTGGGACATGGAATTGGTGGAAAAAGCTTATCAATGGGTTGAAGAAACTGTGGTCTCTAGGCCAAGTTTGAGTGCTTGGCAATCAGCTTTTAGGAGTGCATTGTTGGAAGCTGGGGTTGGTCCTGATAATGGGTTTGATTTGAGGCACCTTGTTGGGACTAAAACTGGAGGGTCCATCTTTGATGACAAAGGAAATAGACATGGAGCTGTAGAACTTCTCAACAAAGCTGAACCCAAAAACCTTAAAGTTGCAACTCAGGCCACAGTCCAAAGAATCATCTTCGATGGTTTATCGGCAAGTGGGGTTTCATATTTTGATTCAAAAGGAAAGTTGCATACAGCATTCATCCACAAGAAAGGAGAGATAATTGTAAGTGCAGGAGCCATTGGAAGCCCTCAACTTCTCCTTCTAAGTGGGGTTGGCCCAAAATCTCATCTTTTGTCTTTAAGACTACCTGTCGTCCTCGACCAACCACATGTTGGAGGATTCATGTCGGACAATCCCCGTTTGAGTCCCACCCTTGTACTTCCATTCCAACTAGTTGCTTCATCTGCACAAATCGTTGGAACATTAGACAACAATATTCACTTGCAATCATTTGCTAGCCCTTTACCATTTTTTGCTCCGCCATCTTTTAGTCTTCTTCCTCCTCAATCCACTTCCATCATCCCTAGCTTAGCCATTTTTGTTGGAAAATTCTCTGATGTCCATTCTGAGGGGTCACTCCGATTGAATTCCTCGACTGATGTGAAGAATAGTCCTATTGTTCGATTCAATTATTATTCACATCCTGATGATCTTGCTCGATGTGTTAGAGGAGTAAGAAAAGTGGGAGATTTGCTTACAACGCAAACCATGGAAAAGATGAAGACCCAAGACATAGAGGGTAATAAAGGATTTCAGTTTCTAGGGCTTCCCTTGCCAGAAAATTTGTGGAATGATAGCTCTGTCGAAGAATACTGTCAGAAGACGGTGACAACTTATTGGCATTATCATGGAGGATGTTTGGTCGGAAAAGTGGTCGATGATAACCACAAAGTCATTGGAATTGAAAATTTACGTGTAGTGGATGGCTCCACTTTCTCTGTCTCACCAGGAACTAATCCTATGGCCACCCTGATGATGCTCGGCCGAGAGAGTAGGAAAATTGGATGCTGCAAACGTTTGACGGCTAGGTCACTTAAGTCAAATTTCGAACTAGCATCGCGGTGCTCCTTGTCTGCAAGCTCACACGCACATTACACAATGATCTATCAAAATTCGTAG

Coding sequence (CDS)

ATGGAGAATTTTATGGCTACTATTCTCCTCTTCATCCTCATCTCTATCTTTCAATTAGGATTTCTCTCCTCCAACACTATCCCCAATCAAGAAAAAGAAGAACATGACTACATAGTAATAGGAGGAGGAACTGCAGGCTGTCCATTAGCTGCGACATTATCATCGAAATTTTCAGTTCTCCTTCTTGAAAGAGGCAGTGATCCTAATAAATATCCTTCTGTGCTAAATGAACAAGGCCTATTGAATGCTTTTGTTGCAGAAGATGATGGCCAAAATCCCTTCCAACATTTCACTTCTGAGGATGGTGTAGAGAACCTAAGAGGGCGTGTTCTCGGTGGTGGTAGCATGATCAATGCTGGCTTCTACTCGAGGGGTCATCGTGAGTTCTTTGAAACTGCAGGTGTTGATTGGGACATGGAATTGGTGGAAAAAGCTTATCAATGGGTTGAAGAAACTGTGGTCTCTAGGCCAAGTTTGAGTGCTTGGCAATCAGCTTTTAGGAGTGCATTGTTGGAAGCTGGGGTTGGTCCTGATAATGGGTTTGATTTGAGGCACCTTGTTGGGACTAAAACTGGAGGGTCCATCTTTGATGACAAAGGAAATAGACATGGAGCTGTAGAACTTCTCAACAAAGCTGAACCCAAAAACCTTAAAGTTGCAACTCAGGCCACAGTCCAAAGAATCATCTTCGATGGTTTATCGGCAAGTGGGGTTTCATATTTTGATTCAAAAGGAAAGTTGCATACAGCATTCATCCACAAGAAAGGAGAGATAATTGTAAGTGCAGGAGCCATTGGAAGCCCTCAACTTCTCCTTCTAAGTGGGGTTGGCCCAAAATCTCATCTTTTGTCTTTAAGACTACCTGTCGTCCTCGACCAACCACATGTTGGAGGATTCATGTCGGACAATCCCCGTTTGAGTCCCACCCTTGTACTTCCATTCCAACTAGTTGCTTCATCTGCACAAATCGTTGGAACATTAGACAACAATATTCACTTGCAATCATTTGCTAGCCCTTTACCATTTTTTGCTCCGCCATCTTTTAGTCTTCTTCCTCCTCAATCCACTTCCATCATCCCTAGCTTAGCCATTTTTGTTGGAAAATTCTCTGATGTCCATTCTGAGGGGTCACTCCGATTGAATTCCTCGACTGATGTGAAGAATAGTCCTATTGTTCGATTCAATTATTATTCACATCCTGATGATCTTGCTCGATGTGTTAGAGGAGTAAGAAAAGTGGGAGATTTGCTTACAACGCAAACCATGGAAAAGATGAAGACCCAAGACATAGAGGGTAATAAAGGATTTCAGTTTCTAGGGCTTCCCTTGCCAGAAAATTTGTGGAATGATAGCTCTGTCGAAGAATACTGTCAGAAGACGGTGACAACTTATTGGCATTATCATGGAGGATGTTTGGTCGGAAAAGTGGTCGATGATAACCACAAAGTCATTGGAATTGAAAATTTACGTGTAGTGGATGGCTCCACTTTCTCTGTCTCACCAGGAACTAATCCTATGGCCACCCTGATGATGCTCGGCCGAGAGAGTAGGAAAATTGGATGCTGCAAACGTTTGACGGCTAGGTCACTTAAGTCAAATTTCGAACTAGCATCGCGGTGCTCCTTGTCTGCAAGCTCACACGCACATTACACAATGATCTATCAAAATTCGTAG

Protein sequence

MENFMATILLFILISIFQLGFLSSNTIPNQEKEEHDYIVIGGGTAGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFTSEDGVENLRGRVLGGGSMINAGFYSRGHREFFETAGVDWDMELVEKAYQWVEETVVSRPSLSAWQSAFRSALLEAGVGPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAEPKNLKVATQATVQRIIFDGLSASGVSYFDSKGKLHTAFIHKKGEIIVSAGAIGSPQLLLLSGVGPKSHLLSLRLPVVLDQPHVGGFMSDNPRLSPTLVLPFQLVASSAQIVGTLDNNIHLQSFASPLPFFAPPSFSLLPPQSTSIIPSLAIFVGKFSDVHSEGSLRLNSSTDVKNSPIVRFNYYSHPDDLARCVRGVRKVGDLLTTQTMEKMKTQDIEGNKGFQFLGLPLPENLWNDSSVEEYCQKTVTTYWHYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRESRKIGCCKRLTARSLKSNFELASRCSLSASSHAHYTMIYQNS
Homology
BLAST of Clc11G13550 vs. NCBI nr
Match: XP_038889699.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 927.9 bits (2397), Expect = 4.0e-266
Identity = 465/529 (87.90%), Postives = 486/529 (91.87%), Query Frame = 0

Query: 1   MENFMATILLFILISIFQLGFLSSNTIPNQ---------------EKEEHDYIVIGGGTA 60
           M+  MATILL ILISIFQLG  SS+TIP+Q               E  E+DYI+IGGGTA
Sbjct: 1   MKRSMATILLIILISIFQLGVFSSHTIPDQDTSYLKFVHEANELPETGEYDYIIIGGGTA 60

Query: 61  GCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFTSEDGVEN 120
           GCPLAATLSSK+SVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHF SEDGVEN
Sbjct: 61  GCPLAATLSSKYSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVEN 120

Query: 121 LRGRVLGGGSMINAGFYSRGHREFFETAGVDWDMELVEKAYQWVEETVVSRPSLSAWQSA 180
           +RGRVLGGGSMINAGFYSRGHREFFETAGVDWDMELVEKAYQWVE+TVVSRPSLSAWQSA
Sbjct: 121 IRGRVLGGGSMINAGFYSRGHREFFETAGVDWDMELVEKAYQWVEQTVVSRPSLSAWQSA 180

Query: 181 FRSALLEAGVGPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAEPKNLKVATQATV 240
           FR ALLEAGVGPDNGFDL+HLVGTKTGGSIFD+KGNRHGAVELLNKAEP+NLKV TQA V
Sbjct: 181 FRGALLEAGVGPDNGFDLKHLVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVGTQAIV 240

Query: 241 QRIIFDGLSASGVSYFDSKGKLHTAFIHKKGEIIVSAGAIGSPQLLLLSGVGPKSHLLSL 300
           +RIIF GLSASGVSY D KGKLHTAFI +KGEII+SAGAIGSPQLLLLSGVGPK HL SL
Sbjct: 241 ERIIFTGLSASGVSYSDPKGKLHTAFIREKGEIILSAGAIGSPQLLLLSGVGPKYHLSSL 300

Query: 301 RLPVVLDQPHVGGFMSDNPRLSPTLVLPFQLVASSAQIVGTLDNNIHLQSFASPLPFFAP 360
           +LPVVL QPHVG FMSDNPR SPT+VLPFQLVASSAQ+VGTL NNIHLQSFASPLPFFAP
Sbjct: 301 KLPVVLHQPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLANNIHLQSFASPLPFFAP 360

Query: 361 PSFSLLPPQSTSIIPSLAIFVGKFSDVHSEGSLRLNSSTDVKNSPIVRFNYYSHPDDLAR 420
           PSFSLLPPQSTSIIPSLAIFVGKFSDV SEGSLRLNSS DVK SPIVRFNYYS+ DDLAR
Sbjct: 361 PSFSLLPPQSTSIIPSLAIFVGKFSDVLSEGSLRLNSSIDVKESPIVRFNYYSNHDDLAR 420

Query: 421 CVRGVRKVGDLLTTQTMEKMKTQDIEGNKGFQFLGLPLPENLWNDSSVEEYCQKTVTTYW 480
           CVRGVRKVGDLL TQTMEK+K QD+EGNKGFQFLGLPLPENLWNDS+VEEYCQKTVTTYW
Sbjct: 421 CVRGVRKVGDLLKTQTMEKIKNQDLEGNKGFQFLGLPLPENLWNDSAVEEYCQKTVTTYW 480

Query: 481 HYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGR 515
           HYHGGCLVGKVVD NHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGR
Sbjct: 481 HYHGGCLVGKVVDHNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGR 529

BLAST of Clc11G13550 vs. NCBI nr
Match: XP_031736478.1 ((R)-mandelonitrile lyase 1-like [Cucumis sativus] >KGN61117.1 hypothetical protein Csa_021176 [Cucumis sativus])

HSP 1 Score: 926.4 bits (2393), Expect = 1.2e-265
Identity = 464/530 (87.55%), Postives = 488/530 (92.08%), Query Frame = 0

Query: 1   MENFMAT-ILLFILISIFQLGFLSSNTIPN---------------QEKEEHDYIVIGGGT 60
           M+N MA  ILLF LISIFQLG ++S+TIPN               QE EE+DYI+IGGGT
Sbjct: 1   MKNSMANQILLFTLISIFQLGLIASHTIPNQDTSYMKFVYEASELQESEEYDYIIIGGGT 60

Query: 61  AGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFTSEDGVE 120
           AGCPLAATLSSKFSVLLLERG+DPNKYPSVLNEQGLLNAFVAEDDGQNPFQHF SEDGVE
Sbjct: 61  AGCPLAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVE 120

Query: 121 NLRGRVLGGGSMINAGFYSRGHREFFETAGVDWDMELVEKAYQWVEETVVSRPSLSAWQS 180
           NLRGRVLGGGSMINAGFYSRGHR+FFETAGVDWD ELVEKAY+WVEETVVS+PSLS WQ+
Sbjct: 121 NLRGRVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSPWQA 180

Query: 181 AFRSALLEAGVGPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAEPKNLKVATQAT 240
           AFRSALLE GVG D GFDLRHLVGTKTGGSIFD+KGNRHGAVELLNK EPKNLKVATQAT
Sbjct: 181 AFRSALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVATQAT 240

Query: 241 VQRIIFDGLSASGVSYFDSKGKLHTAFIHKKGEIIVSAGAIGSPQLLLLSGVGPKSHLLS 300
           VQRIIF GLSASGVSY DSKGKLHTAFI KKGEII+SAGAIGSPQLLLLSGVGPKS+L S
Sbjct: 241 VQRIIFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSYLSS 300

Query: 301 LRLPVVLDQPHVGGFMSDNPRLSPTLVLPFQLVASSAQIVGTLDNNIHLQSFASPLPFFA 360
           LRLPVVLDQPHVG FMSDNPR SPT+VLPFQLV+SSAQ+VGTLD+NIHLQSFASPLPFFA
Sbjct: 301 LRLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQSFASPLPFFA 360

Query: 361 PPSFSLLPPQSTSIIPSLAIFVGKFSDVHSEGSLRLNSSTDVKNSPIVRFNYYSHPDDLA 420
           PPSFSLLPPQ TSI+PSLAIFVGKFSDVHSEGSLRLNSS DVK SPIVRFNYYSHPDDLA
Sbjct: 361 PPSFSLLPPQFTSIVPSLAIFVGKFSDVHSEGSLRLNSSIDVKESPIVRFNYYSHPDDLA 420

Query: 421 RCVRGVRKVGDLLTTQTMEKMKTQDIEGNKGFQFLGLPLPENLWNDSSVEEYCQKTVTTY 480
           RCVRGVRKVGDLL T TMEK+KTQD+EGNK FQFLGL LPENL ND++VEEYCQKTVTTY
Sbjct: 421 RCVRGVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAVEEYCQKTVTTY 480

Query: 481 WHYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGR 515
           WHYHGGCLVGKVVDDNHKVIGI+NLRVVDGSTFSVSPGTNPMATLMMLGR
Sbjct: 481 WHYHGGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGR 530

BLAST of Clc11G13550 vs. NCBI nr
Match: XP_008451922.1 (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo] >KAA0067298.1 (R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa] >TYK10909.1 (R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 923.7 bits (2386), Expect = 7.5e-265
Identity = 460/522 (88.12%), Postives = 483/522 (92.53%), Query Frame = 0

Query: 8   ILLFILISIFQLGFLSSNTIPN---------------QEKEEHDYIVIGGGTAGCPLAAT 67
           ILLF LISIFQLG +SS+ IPN               QE EE+DYI+IGGGTAGCPLAAT
Sbjct: 5   ILLFTLISIFQLGVISSHPIPNQDTSYMKFVHEASELQESEEYDYIIIGGGTAGCPLAAT 64

Query: 68  LSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFTSEDGVENLRGRVLG 127
           LSSKFSVLLLERG+DPNKYPSVLNEQGLLNAFVAEDDGQNPFQHF SEDGVENLRGRVLG
Sbjct: 65  LSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRGRVLG 124

Query: 128 GGSMINAGFYSRGHREFFETAGVDWDMELVEKAYQWVEETVVSRPSLSAWQSAFRSALLE 187
           GGSMINAGFYSRGHREFFETAGVDWDMELVEKAY+WVEETVVS+PSLS WQ+AFRSALLE
Sbjct: 125 GGSMINAGFYSRGHREFFETAGVDWDMELVEKAYEWVEETVVSKPSLSPWQAAFRSALLE 184

Query: 188 AGVGPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAEPKNLKVATQATVQRIIFDG 247
            GVG DNGFDL+HLVGTKTGGSIFD+KGNRHGAVELLNK EP+N KVATQATVQRIIF G
Sbjct: 185 GGVGHDNGFDLKHLVGTKTGGSIFDNKGNRHGAVELLNKGEPENFKVATQATVQRIIFTG 244

Query: 248 LSASGVSYFDSKGKLHTAFIHKKGEIIVSAGAIGSPQLLLLSGVGPKSHLLSLRLPVVLD 307
           LSASGVSY DSKGKLHTAFI KKGEII+SAGAIGSPQLLLLSGVGPKS L SL+LPVVLD
Sbjct: 245 LSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSQLKSLKLPVVLD 304

Query: 308 QPHVGGFMSDNPRLSPTLVLPFQLVASSAQIVGTLDNNIHLQSFASPLPFFAPPSFSLLP 367
           QPHVG FMSDNPR +PT+VLPFQ+VASSAQ+VGTLDNNIHLQ+FASPLPFFAPPSFSLLP
Sbjct: 305 QPHVGEFMSDNPRFTPTIVLPFQVVASSAQVVGTLDNNIHLQAFASPLPFFAPPSFSLLP 364

Query: 368 PQSTSIIPSLAIFVGKFSDVHSEGSLRLNSSTDVKNSPIVRFNYYSHPDDLARCVRGVRK 427
           PQSTSI+PSLAIFVGKFSDV+SEGSLRLNSS DVK SPIVRFNYYSHPDDLARCVRGVRK
Sbjct: 365 PQSTSIVPSLAIFVGKFSDVYSEGSLRLNSSIDVKESPIVRFNYYSHPDDLARCVRGVRK 424

Query: 428 VGDLLTTQTMEKMKTQDIEGNKGFQFLGLPLPENLWNDSSVEEYCQKTVTTYWHYHGGCL 487
           VGDLL T TMEK+KTQD+EGNK FQFLGL LPENL NDS+VEEYCQKTVTTYWHYHGGCL
Sbjct: 425 VGDLLRTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDSAVEEYCQKTVTTYWHYHGGCL 484

Query: 488 VGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGR 515
           VGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGR
Sbjct: 485 VGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGR 526

BLAST of Clc11G13550 vs. NCBI nr
Match: XP_038889315.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 863.2 bits (2229), Expect = 1.2e-246
Identity = 427/530 (80.57%), Postives = 469/530 (88.49%), Query Frame = 0

Query: 1   MENFMATIL-LFILISIFQLGFLSSNTIPNQE---------------KEEHDYIVIGGGT 60
           ME+  +TIL L ++ISIF LG LSS+ IPNQ+               KEE+DYI+IGGGT
Sbjct: 1   MEHSRSTILILSLMISIFHLGLLSSHAIPNQDVGYMKFVHSATDLPAKEEYDYIIIGGGT 60

Query: 61  AGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFTSEDGVE 120
           AGCPL++TLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAF AEDDG+NPFQHF SEDGVE
Sbjct: 61  AGCPLSSTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFAAEDDGKNPFQHFISEDGVE 120

Query: 121 NLRGRVLGGGSMINAGFYSRGHREFFETAGVDWDMELVEKAYQWVEETVVSRPSLSAWQS 180
           N+RGRVLGGGSM+N GFYSRGH++FFETAGVDWDMELVEKAYQWVEETVVS+P L+AWQS
Sbjct: 121 NIRGRVLGGGSMVNVGFYSRGHQQFFETAGVDWDMELVEKAYQWVEETVVSQPILNAWQS 180

Query: 181 AFRSALLEAGVGPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAEPKNLKVATQAT 240
           AFRSA L+AGV PDNGFDLRH VGTK GGSIFD+KGNRHGAVELLNKA P N+KV  +AT
Sbjct: 181 AFRSASLDAGVVPDNGFDLRHRVGTKIGGSIFDNKGNRHGAVELLNKANPTNIKVGIEAT 240

Query: 241 VQRIIFDGLSASGVSYFDSKGKLHTAFIHKKGEIIVSAGAIGSPQLLLLSGVGPKSHLLS 300
           VQRI+F GLSA GV Y DSKGKLH AFI K+GEIIVSAGA+GSPQLLLLSGVGPKSHL S
Sbjct: 241 VQRILFSGLSARGVLYSDSKGKLHRAFIRKEGEIIVSAGAVGSPQLLLLSGVGPKSHLSS 300

Query: 301 LRLPVVLDQPHVGGFMSDNPRLSPTLVLPFQLVASSAQIVGTLDNNIHLQSFASPLPFFA 360
           L+LPVVL QP+VG FMSDNPR    ++LPFQLV SS ++VG L +NI++QSFASP PFFA
Sbjct: 301 LKLPVVLHQPYVGEFMSDNPRFGTNIILPFQLVPSSVKVVGILQDNIYIQSFASPSPFFA 360

Query: 361 PPSFSLLPPQSTSIIPSLAIFVGKFSDVHSEGSLRLNSSTDVKNSPIVRFNYYSHPDDLA 420
           PPSFSLLPPQ  SI+PSLA+FVGKFS+VHSEGSLRLNSST+VK SPIVRFNYYSHPDDLA
Sbjct: 361 PPSFSLLPPQFNSIVPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 420

Query: 421 RCVRGVRKVGDLLTTQTMEKMKTQDIEGNKGFQFLGLPLPENLWNDSSVEEYCQKTVTTY 480
           RCV+GVRK+GDLL TQTMEK+KT D+EGNKGFQFLGLPLPENL NDSSVEEYCQKTVTTY
Sbjct: 421 RCVKGVRKMGDLLKTQTMEKIKTHDLEGNKGFQFLGLPLPENLSNDSSVEEYCQKTVTTY 480

Query: 481 WHYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGR 515
           WHYHGGCLVGKVVD N+KVIGIENLRVVDGSTF  SPGTNPMATLMMLGR
Sbjct: 481 WHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFCDSPGTNPMATLMMLGR 530

BLAST of Clc11G13550 vs. NCBI nr
Match: XP_038889314.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 861.7 bits (2225), Expect = 3.5e-246
Identity = 430/530 (81.13%), Postives = 468/530 (88.30%), Query Frame = 0

Query: 1   MENFMATIL-LFILISIFQLGFLSSNTIPNQE---------------KEEHDYIVIGGGT 60
           ME+  ATIL L ++ISI QLG L+ N IP ++               KEE+DYI+IGGGT
Sbjct: 1   MEHSKATILILSLMISISQLGVLTYNAIPIKDVRYMKFVHKASDLPAKEEYDYIIIGGGT 60

Query: 61  AGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFTSEDGVE 120
           AGCPLAATLSSKFSVL+LERGSDPNKYPSVLNEQGLLN F A DDG+NPFQ F SEDGVE
Sbjct: 61  AGCPLAATLSSKFSVLVLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFISEDGVE 120

Query: 121 NLRGRVLGGGSMINAGFYSRGHREFFETAGVDWDMELVEKAYQWVEETVVSRPSLSAWQS 180
           N+R RVLGGGSM+NAGFYSRGH++FFETAGVDWDMELVEKAYQWVEETV SRP L+AWQS
Sbjct: 121 NIRARVLGGGSMVNAGFYSRGHQQFFETAGVDWDMELVEKAYQWVEETVASRPILNAWQS 180

Query: 181 AFRSALLEAGVGPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAEPKNLKVATQAT 240
           AFRSALLEAGV PDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAEP N+KVA +AT
Sbjct: 181 AFRSALLEAGVVPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAEPTNIKVAIEAT 240

Query: 241 VQRIIFDGLSASGVSYFDSKGKLHTAFIHKKGEIIVSAGAIGSPQLLLLSGVGPKSHLLS 300
           VQ+I+F GLSA GV Y DSKGKLH AFI KKGEIIVSAGAIGSPQLLLLSGVGPKSHL S
Sbjct: 241 VQKILFSGLSARGVLYSDSKGKLHRAFIRKKGEIIVSAGAIGSPQLLLLSGVGPKSHLSS 300

Query: 301 LRLPVVLDQPHVGGFMSDNPRLSPTLVLPFQLVASSAQIVGTLDNNIHLQSFASPLPFFA 360
           L+LPVVL QP+VG FMSDNPR    ++LPFQLV SS ++VG L +NI++QSFASP PFFA
Sbjct: 301 LKLPVVLHQPYVGEFMSDNPRFGTNIILPFQLVPSSVKVVGILQDNIYIQSFASPSPFFA 360

Query: 361 PPSFSLLPPQSTSIIPSLAIFVGKFSDVHSEGSLRLNSSTDVKNSPIVRFNYYSHPDDLA 420
           PPSFSLLPPQ  SI+PSLA+FVGKFS+VHSEGSLRLNSST+VK SPIVRFNYYSHPDDLA
Sbjct: 361 PPSFSLLPPQFNSIVPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 420

Query: 421 RCVRGVRKVGDLLTTQTMEKMKTQDIEGNKGFQFLGLPLPENLWNDSSVEEYCQKTVTTY 480
           RCV+GVRK+GDLL TQTMEK+KT D+EGNKGFQFLGLPLPENL NDSSV EYCQKTVTTY
Sbjct: 421 RCVKGVRKMGDLLKTQTMEKIKTHDLEGNKGFQFLGLPLPENLSNDSSVGEYCQKTVTTY 480

Query: 481 WHYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGR 515
           WHYHGGCLVGKVVD N+KVIGI+NLRVVDGSTFS SPGTNPMATLMMLGR
Sbjct: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 530

BLAST of Clc11G13550 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 1.3e-142
Identity = 250/484 (51.65%), Postives = 340/484 (70.25%), Query Frame = 0

Query: 35  HDYIVIGGGTAGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGQNPF 94
           +DY+++GGGT+GCPLAATLS K+ VL+LERGS P  YP+VL   G +     EDDG+ P 
Sbjct: 54  YDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPV 113

Query: 95  QHFTSEDGVENLRGRVLGGGSMINAGFYSRGHREFFETAGVDWDMELVEKAYQWVEETVV 154
           + F SEDG++N+RGRVLGG SMINAG Y+R +   +  +GVDWDM+LV K Y+WVE+T+V
Sbjct: 114 ERFVSEDGIDNVRGRVLGGTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIV 173

Query: 155 SRPSLSAWQSAFRSALLEAGVGPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAEP 214
            +P+   WQS   +A LEAGV P++GF L H  GT+  GS FD+KG RH A ELLNK   
Sbjct: 174 FKPNYQPWQSVTGTAFLEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNS 233

Query: 215 KNLKVATQATVQRIIFD---GLSASGVSYFDSKGKLHTAFIHKKGEIIVSAGAIGSPQLL 274
            NL+V   A+V++IIF    GL+A+GV Y DS G  H AF+  KGE+IVSAG IG+PQLL
Sbjct: 234 NNLRVGVHASVEKIIFSNAPGLTATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLL 293

Query: 275 LLSGVGPKSHLLSLRLPVVLDQPHVGGFMSDNPRLSPTLVLPFQLVASSAQIVGTLDNNI 334
           LLSGVGP+S+L SL +PVVL  P+VG F+ DNPR    ++ P  +  +   ++G + N+ 
Sbjct: 294 LLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLG-ISNDF 353

Query: 335 HLQSFASPLPFFAPPSFSLLPPQSTSIIPS-LAIFVGKFSDVHSEGSLRLNSSTDVKNSP 394
           +  SF+S LPF  PP FS  P  S  +  S  A F  K +   S GSL L SS++V+ SP
Sbjct: 354 YQCSFSS-LPFTTPP-FSFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSP 413

Query: 395 IVRFNYYSHPDDLARCVRGVRKVGDLLTTQTMEKMKTQDIEGNKGFQFLGLPLPENLWND 454
            V+FNYYS+P DL+ CV G++K+G+LL+T  ++  K +D+ G +GF  LG+PLP++  +D
Sbjct: 414 NVKFNYYSNPTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDD 473

Query: 455 SSVEEYCQKTVTTYWHYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLM 514
           ++ E +C+++V +YWHYHGGCLVGKV+D + +V GI+ LRVVDGSTF  +P ++P    +
Sbjct: 474 AAFETFCRESVASYWHYHGGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHPQGFYL 533

BLAST of Clc11G13550 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 1.6e-140
Identity = 250/501 (49.90%), Postives = 338/501 (67.47%), Query Frame = 0

Query: 19  LGFLSSNTIPNQEKEEHDYIVIGGGTAGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQ 78
           L F+   T P  E   +DYI++GGGTAGCPLAATLS+ +SVL+LERGS P +YP++L   
Sbjct: 39  LSFVYDATDPELE-GSYDYIIVGGGTAGCPLAATLSANYSVLVLERGSLPTEYPNLLISD 98

Query: 79  GLLNAFVAEDDGQNPFQHFTSEDGVENLRGRVLGGGSMINAGFYSRGHREFFETAGVDWD 138
           G +     EDDG+ P + F SEDG++N+RGRVLGG SMINAG Y R +  FF   G++WD
Sbjct: 99  GFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSMINAGVYVRANTSFFNQTGIEWD 158

Query: 139 MELVEKAYQWVEETVVSRPSLSAWQSAFRSALLEAGVGPDNGFDLRHLVGTKTGGSIFDD 198
           M+LV + Y+WVE+T+V  P    WQ+   +A LEAG+ P+NGF + HL GT+  GS FD+
Sbjct: 159 MDLVNQTYEWVEDTIVFEPDSQTWQTVIGTAYLEAGILPNNGFSVDHLAGTRLTGSTFDN 218

Query: 199 KGNRHGAVELLNKAEPKNLKVATQATVQRIIF----DGLSASGVSYFDSKGKLHTAFIHK 258
            G RH + ELLNK +P NL+VA QA V++IIF     G++A GV Y DS G  H AF+  
Sbjct: 219 NGTRHASDELLNKGDPNNLRVAVQAAVEKIIFSSNTSGVTAIGVIYTDSNGTTHQAFVRG 278

Query: 259 KGEIIVSAGAIGSPQLLLLSGVGPKSHLLSLRLPVVLDQPHVGGFMSDNPRLSPTLVLPF 318
           +GE+I+SAG IGSPQLLLLSGVGP+S+L SL + VV   P+VG ++ DNPR    ++ P 
Sbjct: 279 EGEVILSAGPIGSPQLLLLSGVGPESYLTSLNISVVASHPYVGQYIYDNPRNFINILPPN 338

Query: 319 QLVASSAQIVGTLDNNIHLQSFASPLPFFAPPSFSLLPPQSTSI-IPSLAIFVGKFSDVH 378
            + AS+  ++G   +    Q   S LPF  PP FS  P  S  +   + A  V K     
Sbjct: 339 PIEASTVTVLGITSD--FYQCSISSLPFDTPP-FSFFPTTSYPLPNQTFAHIVNKVPGPL 398

Query: 379 SEGSLRLNSSTDVKNSPIVRFNYYSHPDDLARCVRGVRKVGDLLTTQTMEKMKTQDIEGN 438
           S G++ LNSS+DV+  P V+FNYYS+  DL+ CV G++K+G++L+T  +E  K +D+ G 
Sbjct: 399 SHGTVTLNSSSDVRVGPNVKFNYYSNLTDLSHCVSGMKKLGEVLSTDALEPYKVEDLPGI 458

Query: 439 KGFQFLGLPLPENLWNDSSVEEYCQKTVTTYWHYHGGCLVGKVVDDNHKVIGIENLRVVD 498
            GF  LG+PLPEN  +D++ E +C+++V +YWHYHGGCLVGKV+DD  +V GI  LRVVD
Sbjct: 459 DGFNILGIPLPENQTDDAAFETFCRESVASYWHYHGGCLVGKVLDDGFRVTGINALRVVD 518

Query: 499 GSTFSVSPGTNPMATLMMLGR 515
           GSTF  +P ++P    +MLGR
Sbjct: 519 GSTFPSTPASHPQGFYLMLGR 535

BLAST of Clc11G13550 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 4.6e-140
Identity = 246/484 (50.83%), Postives = 339/484 (70.04%), Query Frame = 0

Query: 35  HDYIVIGGGTAGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGQNPF 94
           +DY+++GGGT+GCPLAATLS K+ VL+LERGS P  YP+VL   G +     EDDG+ P 
Sbjct: 54  YDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPV 113

Query: 95  QHFTSEDGVENLRGRVLGGGSMINAGFYSRGHREFFETAGVDWDMELVEKAYQWVEETVV 154
           + F SEDG++N+RGRVLGG S+INAG Y+R +   +  +GVDWDM+LV + Y+WVE+T+V
Sbjct: 114 ERFVSEDGIDNVRGRVLGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIV 173

Query: 155 SRPSLSAWQSAFRSALLEAGVGPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAEP 214
            +P+  +WQS  ++A LEAGV P++GF L H  GT+  GS FD+KG RH A ELLNK   
Sbjct: 174 YKPNSQSWQSVTKTAFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNS 233

Query: 215 KNLKVATQATVQRIIFD---GLSASGVSYFDSKGKLHTAFIHKKGEIIVSAGAIGSPQLL 274
            NL+V   A+V++IIF    GL+A+GV Y DS G  H AF+  KGE+IVSAG IG+PQLL
Sbjct: 234 NNLRVGVHASVEKIIFSNAPGLTATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLL 293

Query: 275 LLSGVGPKSHLLSLRLPVVLDQPHVGGFMSDNPRLSPTLVLPFQLVASSAQIVGTLDNNI 334
           LLSGVGP+S+L SL +PVVL  P+VG F+ DNPR    ++ P  +  +   ++G + N+ 
Sbjct: 294 LLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLG-ISNDF 353

Query: 335 HLQSFASPLPFFAPPSFSLLPPQSTSIIPS-LAIFVGKFSDVHSEGSLRLNSSTDVKNSP 394
           +  SF+S LPF  PP F   P  S  +  S  A F  K +   S GSL L SS++V+ SP
Sbjct: 354 YQCSFSS-LPFTTPP-FGFFPSASYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSP 413

Query: 395 IVRFNYYSHPDDLARCVRGVRKVGDLLTTQTMEKMKTQDIEGNKGFQFLGLPLPENLWND 454
            V+FNYYS+  DL+ CV G++K+G+LL+T  ++  K +D+ G +GF  LG+PLP++  +D
Sbjct: 414 NVKFNYYSNLTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDD 473

Query: 455 SSVEEYCQKTVTTYWHYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLM 514
           ++ E +C+++V +YWHYHGGCLVGKV+D + +V GI  LRVVDGSTF  +P ++P    +
Sbjct: 474 AAFETFCRESVASYWHYHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHPQGFYL 533

BLAST of Clc11G13550 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 2.3e-139
Identity = 249/500 (49.80%), Postives = 335/500 (67.00%), Query Frame = 0

Query: 24  SNTIPNQEKEEHDYIVIGGGTAGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNA 83
           SN      ++ +DYI++GGGTAGCPLAATLS  F VLLLERG  P   P+V++  G L  
Sbjct: 43  SNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMSHDGFLTT 102

Query: 84  FVAEDDGQNPFQHFTSEDGVENLRGRVLGGGSMINAGFYSRGHREFFETAGVDWDMELVE 143
               ++  +P Q F SE+GV N RGRVLGG S INAGFYSR  ++FFE +G+ WD+  V 
Sbjct: 103 LTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENSGLVWDLSSVN 162

Query: 144 KAYQWVEETVVSRPSLSAWQSAFRSALLEAGVGPDNGFDLRHLVGTKTGGSIFDDKGNRH 203
           ++Y+WVE  +V RP L  WQ+A R ALLE GV P NGF L H VGTK GGS FD  G RH
Sbjct: 163 QSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRH 222

Query: 204 GAVELLNKAEPKNLKVATQATVQRIIF--------DGLSASGVSYFDSKGKLHTAFIHKK 263
            + +LL  A   N++VA  ATV+R++           +SA GV Y D  G+ H A I  +
Sbjct: 223 SSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDR 282

Query: 264 GEIIVSAGAIGSPQLLLLSGVGPKSHLLSLRLPVVLDQPHVGGFMSDNPRLSPTLVLPFQ 323
           GE+I+SAGA+GSPQLL LSG+GP+S+L +  +PV LDQPHVG F+ DNPR   ++V P  
Sbjct: 283 GEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVP 342

Query: 324 LVASSAQIVGTLDNNIHLQSFASPLPFFAP-PSFSLLPPQSTSIIPSLAIFVGKFSDVHS 383
           +  S  Q+VG  ++   L++ ++ +PF +P  S  +  P S   +P   I + K     S
Sbjct: 343 MENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPASPLYVPVTTI-MEKILGPVS 402

Query: 384 EGSLRLNSSTDVKNSPIVRFNYYSHPDDLARCVRGVRKVGDLLTTQTMEKMKTQDIEGNK 443
            G LRL +STDV+ +P+VRFNY+S P DL RCV G RK+G++L ++ M+    ++  GN+
Sbjct: 403 IGLLRL-ASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIREWFGNR 462

Query: 444 GFQFLGLPLPENLWNDSSVEEYCQKTVTTYWHYHGGCLVGKVVDDNHKVIGIENLRVVDG 503
            F+F+G PLP +  ND  + ++C++TV+T WHYHGG +VGKVVD + KVIG+ +LR+VDG
Sbjct: 463 RFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDG 522

Query: 504 STFSVSPGTNPMATLMMLGR 515
           STF++SPGTNP ATLMMLGR
Sbjct: 523 STFNISPGTNPQATLMMLGR 540

BLAST of Clc11G13550 vs. ExPASy Swiss-Prot
Match: O50048 ((R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 1.3e-134
Identity = 239/486 (49.18%), Postives = 325/486 (66.87%), Query Frame = 0

Query: 35  HDYIVIGGGTAGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGQNPF 94
           +DYI++GGGTAGCPLAATLS+ +SVL+LERG+ P +YP++L   G +     EDDGQ P 
Sbjct: 55  YDYIIVGGGTAGCPLAATLSANYSVLVLERGTLPTEYPNLLTSDGFIYNLQQEDDGQTPV 114

Query: 95  QHFTSEDGVENLRGRVLGGGSMINAGFYSRGHREFFETAGVDWDMELVEKAYQWVEETVV 154
           + F S DG++N+RGRVLGG SMINAG Y R +  FF   G++WDM+LV K Y WVE+T+V
Sbjct: 115 ERFVSGDGIDNVRGRVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNKTYDWVEDTIV 174

Query: 155 SRPSLSAWQSAFRSALLEAGVGPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAEP 214
            +P    WQ+   +A LE G+ PDNGF L HL GT+  GS FD+ G RH + ELLNK +P
Sbjct: 175 FKPDFQFWQNLTGTAFLEVGILPDNGFSLDHLEGTRLTGSTFDNNGTRHASDELLNKGDP 234

Query: 215 KNLKVATQATVQRIIF----DGLSASGVSYFDSKGKLHTAFIHKKGEIIVSAGAIGSPQL 274
            NL+VA  A V++IIF     G++A GV Y DS G  H AF+   GE+I+SAG IGSPQL
Sbjct: 235 NNLRVAVHAAVEKIIFSSDSSGVTAIGVIYTDSNGTTHQAFVRGDGEVILSAGPIGSPQL 294

Query: 275 LLLSGVGPKSHLLSLRLPVVLDQPHVGGFMSDNPRLSPTLVLPFQLVASSAQIVGTLDNN 334
           LLLSGVG +S+L SL + VV   P+VG ++ DNPR    ++ P  + AS+  ++G   + 
Sbjct: 295 LLLSGVGLESYLTSLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSD- 354

Query: 335 IHLQSFASPLPFFAPPSFSLLPPQSTSIIP--SLAIFVGKFSDVHSEGSLRLNSSTDVKN 394
              Q   S LPF   P F    P  T  +P  + A  V K     S G++ L S++DV+ 
Sbjct: 355 -FYQCSISSLPFSTAP-FGFF-PNPTYPLPNTTFAHIVNKVPGPLSHGTVLLQSTSDVRV 414

Query: 395 SPIVRFNYYSHPDDLARCVRGVRKVGDLLTTQTMEKMKTQDIEGNKGFQFLGLPLPENLW 454
           +P V FNYYS+  DLA CV G++K+G+ L++  ++  K +D+ G +GF  LG+PLPEN  
Sbjct: 415 APNVTFNYYSNTTDLAHCVSGMKKIGEFLSSDALKPYKVEDLPGIEGFDILGIPLPENQT 474

Query: 455 NDSSVEEYCQKTVTTYWHYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMAT 514
           +D++ E +C++ V +YWHYHGGCLVG+V+DD+ +V GI  LRVVDGSTF  +P ++P   
Sbjct: 475 DDAAFETFCREAVASYWHYHGGCLVGEVLDDDFRVTGINALRVVDGSTFPSTPASHPQGF 534

BLAST of Clc11G13550 vs. ExPASy TrEMBL
Match: A0A0A0LK79 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055050 PE=3 SV=1)

HSP 1 Score: 926.4 bits (2393), Expect = 5.6e-266
Identity = 464/530 (87.55%), Postives = 488/530 (92.08%), Query Frame = 0

Query: 1   MENFMAT-ILLFILISIFQLGFLSSNTIPN---------------QEKEEHDYIVIGGGT 60
           M+N MA  ILLF LISIFQLG ++S+TIPN               QE EE+DYI+IGGGT
Sbjct: 1   MKNSMANQILLFTLISIFQLGLIASHTIPNQDTSYMKFVYEASELQESEEYDYIIIGGGT 60

Query: 61  AGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFTSEDGVE 120
           AGCPLAATLSSKFSVLLLERG+DPNKYPSVLNEQGLLNAFVAEDDGQNPFQHF SEDGVE
Sbjct: 61  AGCPLAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVE 120

Query: 121 NLRGRVLGGGSMINAGFYSRGHREFFETAGVDWDMELVEKAYQWVEETVVSRPSLSAWQS 180
           NLRGRVLGGGSMINAGFYSRGHR+FFETAGVDWD ELVEKAY+WVEETVVS+PSLS WQ+
Sbjct: 121 NLRGRVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSPWQA 180

Query: 181 AFRSALLEAGVGPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAEPKNLKVATQAT 240
           AFRSALLE GVG D GFDLRHLVGTKTGGSIFD+KGNRHGAVELLNK EPKNLKVATQAT
Sbjct: 181 AFRSALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVATQAT 240

Query: 241 VQRIIFDGLSASGVSYFDSKGKLHTAFIHKKGEIIVSAGAIGSPQLLLLSGVGPKSHLLS 300
           VQRIIF GLSASGVSY DSKGKLHTAFI KKGEII+SAGAIGSPQLLLLSGVGPKS+L S
Sbjct: 241 VQRIIFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSYLSS 300

Query: 301 LRLPVVLDQPHVGGFMSDNPRLSPTLVLPFQLVASSAQIVGTLDNNIHLQSFASPLPFFA 360
           LRLPVVLDQPHVG FMSDNPR SPT+VLPFQLV+SSAQ+VGTLD+NIHLQSFASPLPFFA
Sbjct: 301 LRLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQSFASPLPFFA 360

Query: 361 PPSFSLLPPQSTSIIPSLAIFVGKFSDVHSEGSLRLNSSTDVKNSPIVRFNYYSHPDDLA 420
           PPSFSLLPPQ TSI+PSLAIFVGKFSDVHSEGSLRLNSS DVK SPIVRFNYYSHPDDLA
Sbjct: 361 PPSFSLLPPQFTSIVPSLAIFVGKFSDVHSEGSLRLNSSIDVKESPIVRFNYYSHPDDLA 420

Query: 421 RCVRGVRKVGDLLTTQTMEKMKTQDIEGNKGFQFLGLPLPENLWNDSSVEEYCQKTVTTY 480
           RCVRGVRKVGDLL T TMEK+KTQD+EGNK FQFLGL LPENL ND++VEEYCQKTVTTY
Sbjct: 421 RCVRGVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAVEEYCQKTVTTY 480

Query: 481 WHYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGR 515
           WHYHGGCLVGKVVDDNHKVIGI+NLRVVDGSTFSVSPGTNPMATLMMLGR
Sbjct: 481 WHYHGGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGR 530

BLAST of Clc11G13550 vs. ExPASy TrEMBL
Match: A0A5D3CL00 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00890 PE=3 SV=1)

HSP 1 Score: 923.7 bits (2386), Expect = 3.7e-265
Identity = 460/522 (88.12%), Postives = 483/522 (92.53%), Query Frame = 0

Query: 8   ILLFILISIFQLGFLSSNTIPN---------------QEKEEHDYIVIGGGTAGCPLAAT 67
           ILLF LISIFQLG +SS+ IPN               QE EE+DYI+IGGGTAGCPLAAT
Sbjct: 5   ILLFTLISIFQLGVISSHPIPNQDTSYMKFVHEASELQESEEYDYIIIGGGTAGCPLAAT 64

Query: 68  LSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFTSEDGVENLRGRVLG 127
           LSSKFSVLLLERG+DPNKYPSVLNEQGLLNAFVAEDDGQNPFQHF SEDGVENLRGRVLG
Sbjct: 65  LSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRGRVLG 124

Query: 128 GGSMINAGFYSRGHREFFETAGVDWDMELVEKAYQWVEETVVSRPSLSAWQSAFRSALLE 187
           GGSMINAGFYSRGHREFFETAGVDWDMELVEKAY+WVEETVVS+PSLS WQ+AFRSALLE
Sbjct: 125 GGSMINAGFYSRGHREFFETAGVDWDMELVEKAYEWVEETVVSKPSLSPWQAAFRSALLE 184

Query: 188 AGVGPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAEPKNLKVATQATVQRIIFDG 247
            GVG DNGFDL+HLVGTKTGGSIFD+KGNRHGAVELLNK EP+N KVATQATVQRIIF G
Sbjct: 185 GGVGHDNGFDLKHLVGTKTGGSIFDNKGNRHGAVELLNKGEPENFKVATQATVQRIIFTG 244

Query: 248 LSASGVSYFDSKGKLHTAFIHKKGEIIVSAGAIGSPQLLLLSGVGPKSHLLSLRLPVVLD 307
           LSASGVSY DSKGKLHTAFI KKGEII+SAGAIGSPQLLLLSGVGPKS L SL+LPVVLD
Sbjct: 245 LSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSQLKSLKLPVVLD 304

Query: 308 QPHVGGFMSDNPRLSPTLVLPFQLVASSAQIVGTLDNNIHLQSFASPLPFFAPPSFSLLP 367
           QPHVG FMSDNPR +PT+VLPFQ+VASSAQ+VGTLDNNIHLQ+FASPLPFFAPPSFSLLP
Sbjct: 305 QPHVGEFMSDNPRFTPTIVLPFQVVASSAQVVGTLDNNIHLQAFASPLPFFAPPSFSLLP 364

Query: 368 PQSTSIIPSLAIFVGKFSDVHSEGSLRLNSSTDVKNSPIVRFNYYSHPDDLARCVRGVRK 427
           PQSTSI+PSLAIFVGKFSDV+SEGSLRLNSS DVK SPIVRFNYYSHPDDLARCVRGVRK
Sbjct: 365 PQSTSIVPSLAIFVGKFSDVYSEGSLRLNSSIDVKESPIVRFNYYSHPDDLARCVRGVRK 424

Query: 428 VGDLLTTQTMEKMKTQDIEGNKGFQFLGLPLPENLWNDSSVEEYCQKTVTTYWHYHGGCL 487
           VGDLL T TMEK+KTQD+EGNK FQFLGL LPENL NDS+VEEYCQKTVTTYWHYHGGCL
Sbjct: 425 VGDLLRTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDSAVEEYCQKTVTTYWHYHGGCL 484

Query: 488 VGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGR 515
           VGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGR
Sbjct: 485 VGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGR 526

BLAST of Clc11G13550 vs. ExPASy TrEMBL
Match: A0A1S3BSP4 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493073 PE=3 SV=1)

HSP 1 Score: 923.7 bits (2386), Expect = 3.7e-265
Identity = 460/522 (88.12%), Postives = 483/522 (92.53%), Query Frame = 0

Query: 8   ILLFILISIFQLGFLSSNTIPN---------------QEKEEHDYIVIGGGTAGCPLAAT 67
           ILLF LISIFQLG +SS+ IPN               QE EE+DYI+IGGGTAGCPLAAT
Sbjct: 5   ILLFTLISIFQLGVISSHPIPNQDTSYMKFVHEASELQESEEYDYIIIGGGTAGCPLAAT 64

Query: 68  LSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFTSEDGVENLRGRVLG 127
           LSSKFSVLLLERG+DPNKYPSVLNEQGLLNAFVAEDDGQNPFQHF SEDGVENLRGRVLG
Sbjct: 65  LSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRGRVLG 124

Query: 128 GGSMINAGFYSRGHREFFETAGVDWDMELVEKAYQWVEETVVSRPSLSAWQSAFRSALLE 187
           GGSMINAGFYSRGHREFFETAGVDWDMELVEKAY+WVEETVVS+PSLS WQ+AFRSALLE
Sbjct: 125 GGSMINAGFYSRGHREFFETAGVDWDMELVEKAYEWVEETVVSKPSLSPWQAAFRSALLE 184

Query: 188 AGVGPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAEPKNLKVATQATVQRIIFDG 247
            GVG DNGFDL+HLVGTKTGGSIFD+KGNRHGAVELLNK EP+N KVATQATVQRIIF G
Sbjct: 185 GGVGHDNGFDLKHLVGTKTGGSIFDNKGNRHGAVELLNKGEPENFKVATQATVQRIIFTG 244

Query: 248 LSASGVSYFDSKGKLHTAFIHKKGEIIVSAGAIGSPQLLLLSGVGPKSHLLSLRLPVVLD 307
           LSASGVSY DSKGKLHTAFI KKGEII+SAGAIGSPQLLLLSGVGPKS L SL+LPVVLD
Sbjct: 245 LSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSQLKSLKLPVVLD 304

Query: 308 QPHVGGFMSDNPRLSPTLVLPFQLVASSAQIVGTLDNNIHLQSFASPLPFFAPPSFSLLP 367
           QPHVG FMSDNPR +PT+VLPFQ+VASSAQ+VGTLDNNIHLQ+FASPLPFFAPPSFSLLP
Sbjct: 305 QPHVGEFMSDNPRFTPTIVLPFQVVASSAQVVGTLDNNIHLQAFASPLPFFAPPSFSLLP 364

Query: 368 PQSTSIIPSLAIFVGKFSDVHSEGSLRLNSSTDVKNSPIVRFNYYSHPDDLARCVRGVRK 427
           PQSTSI+PSLAIFVGKFSDV+SEGSLRLNSS DVK SPIVRFNYYSHPDDLARCVRGVRK
Sbjct: 365 PQSTSIVPSLAIFVGKFSDVYSEGSLRLNSSIDVKESPIVRFNYYSHPDDLARCVRGVRK 424

Query: 428 VGDLLTTQTMEKMKTQDIEGNKGFQFLGLPLPENLWNDSSVEEYCQKTVTTYWHYHGGCL 487
           VGDLL T TMEK+KTQD+EGNK FQFLGL LPENL NDS+VEEYCQKTVTTYWHYHGGCL
Sbjct: 425 VGDLLRTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDSAVEEYCQKTVTTYWHYHGGCL 484

Query: 488 VGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGR 515
           VGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGR
Sbjct: 485 VGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGR 526

BLAST of Clc11G13550 vs. ExPASy TrEMBL
Match: A0A6J1K4X8 ((R)-mandelonitrile lyase 1-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111492330 PE=3 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 2.9e-246
Identity = 418/529 (79.02%), Postives = 470/529 (88.85%), Query Frame = 0

Query: 1   MENFMATILLFILISIFQLGFLSSNTIPNQ---------------EKEEHDYIVIGGGTA 60
           ME  MATIL  ILIS FQ G +SS TIP+Q               EK+E+DYI++GGG A
Sbjct: 1   MERSMATILFLILISTFQFGAISSTTIPDQDDSYMKLVYNAGDLPEKQEYDYIIVGGGAA 60

Query: 61  GCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFTSEDGVEN 120
           GCPLAATLSSKF+VLLLERGS+PNKYPSVLNEQGLLNAF+A+DDGQNPFQ FTSEDGVEN
Sbjct: 61  GCPLAATLSSKFNVLLLERGSEPNKYPSVLNEQGLLNAFLAQDDGQNPFQRFTSEDGVEN 120

Query: 121 LRGRVLGGGSMINAGFYSRGHREFFETAGVDWDMELVEKAYQWVEETVVSRPSLSAWQSA 180
           +RGR+LGGG+M+NAGFYSRGHR+FFETAGV+WDME+VE AYQWVEETVVS+P L+AWQSA
Sbjct: 121 IRGRILGGGTMVNAGFYSRGHRQFFETAGVNWDMEMVENAYQWVEETVVSKPILNAWQSA 180

Query: 181 FRSALLEAGVGPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAEPKNLKVATQATV 240
           F+SALLEAGVGPDNGF+L HL+GTKTGGSIFD KGNRHGAVELLNKAEPKNLKVA  ATV
Sbjct: 181 FKSALLEAGVGPDNGFNLTHLIGTKTGGSIFDSKGNRHGAVELLNKAEPKNLKVAVNATV 240

Query: 241 QRIIFDGLSASGVSYFDSKGKLHTAFIHKKGEIIVSAGAIGSPQLLLLSGVGPKSHLLSL 300
           +RI+F+GLSA+GVSY DSKGK+HTAFI KKGEI +SAGAIGSP LLL SGVGPKSHL SL
Sbjct: 241 RRILFNGLSANGVSYSDSKGKIHTAFIRKKGEIFLSAGAIGSPLLLLQSGVGPKSHLSSL 300

Query: 301 RLPVVLDQPHVGGFMSDNPRLSPTLVLPFQLVASSAQIVGTLDNNIHLQSFASPLPFFAP 360
           +LPVV  QP+VG FMSDNPR   T+VLPFQL +SS ++VGTL +N+HLQ+FASP PF AP
Sbjct: 301 KLPVVHHQPYVGEFMSDNPRFGATIVLPFQLASSSVKVVGTLQDNVHLQAFASPAPFLAP 360

Query: 361 PSFSLLPPQSTSIIPSLAIFVGKFSDVHSEGSLRLNSSTDVKNSPIVRFNYYSHPDDLAR 420
           P+FSLLPPQ+TSI PSL IFVGKFS+VHSEG LRLNS+TD KN+ IVRFNYYSHPDDLAR
Sbjct: 361 PTFSLLPPQATSINPSLVIFVGKFSEVHSEGFLRLNSTTDAKNNVIVRFNYYSHPDDLAR 420

Query: 421 CVRGVRKVGDLLTTQTMEKMKTQDIEGNKGFQFLGLPLPENLWNDSSVEEYCQKTVTTYW 480
           CV GVRKVGDLL TQTMEK+KTQD+EGNKGFQF+G+PLPENLWN SSVEEYC+ TVTTYW
Sbjct: 421 CVEGVRKVGDLLKTQTMEKIKTQDLEGNKGFQFIGVPLPENLWNVSSVEEYCRGTVTTYW 480

Query: 481 HYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGR 515
           HYHGGCLVGKVVD +++VIG++NLRVVDGSTFS SPGTNPMATLMMLGR
Sbjct: 481 HYHGGCLVGKVVDGDYRVIGMKNLRVVDGSTFSDSPGTNPMATLMMLGR 529

BLAST of Clc11G13550 vs. ExPASy TrEMBL
Match: A0A6J1EP46 ((R)-mandelonitrile lyase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436113 PE=3 SV=1)

HSP 1 Score: 854.4 bits (2206), Expect = 2.7e-244
Identity = 416/529 (78.64%), Postives = 469/529 (88.66%), Query Frame = 0

Query: 1   MENFMATILLFILISIFQLGFLSSNTIPNQ---------------EKEEHDYIVIGGGTA 60
           ME  +ATIL  ILIS FQ G +SSNTIP+Q               EK+E+DYI+IGGG A
Sbjct: 1   MERSIATILFLILISTFQFGAISSNTIPDQDVSYMKFVHNAGDLPEKQEYDYIIIGGGAA 60

Query: 61  GCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFTSEDGVEN 120
           GCPLAATLSSKF VLLLERGS+PNKYPSVLNEQGLLNAF+A+DDGQNPFQ FTSEDGVEN
Sbjct: 61  GCPLAATLSSKFKVLLLERGSEPNKYPSVLNEQGLLNAFLAQDDGQNPFQRFTSEDGVEN 120

Query: 121 LRGRVLGGGSMINAGFYSRGHREFFETAGVDWDMELVEKAYQWVEETVVSRPSLSAWQSA 180
           +RGR+LGG +M+NAGFYSRGHR+FFETAGV+WDME+VE AYQWVEETVVS+P L+AWQSA
Sbjct: 121 IRGRILGGSTMVNAGFYSRGHRQFFETAGVNWDMEMVENAYQWVEETVVSKPILNAWQSA 180

Query: 181 FRSALLEAGVGPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAEPKNLKVATQATV 240
           F+SALLEAGVGPDNGF+L HL+GTKTGGSIFD KGNRHGAVELLNKAEP NLKVA  ATV
Sbjct: 181 FKSALLEAGVGPDNGFNLTHLIGTKTGGSIFDGKGNRHGAVELLNKAEPMNLKVAVNATV 240

Query: 241 QRIIFDGLSASGVSYFDSKGKLHTAFIHKKGEIIVSAGAIGSPQLLLLSGVGPKSHLLSL 300
           ++I+F+GLSA+GVSY DSKGK+HTAFI KKGEI +SAGAIGSP LLL SGVGPKSHL SL
Sbjct: 241 RQILFNGLSANGVSYSDSKGKIHTAFIRKKGEIFLSAGAIGSPLLLLQSGVGPKSHLSSL 300

Query: 301 RLPVVLDQPHVGGFMSDNPRLSPTLVLPFQLVASSAQIVGTLDNNIHLQSFASPLPFFAP 360
           +LPVV  QP+VG FMSDNPR   T+VLPFQL +SS ++VGTL +N+HLQ+FASP PF AP
Sbjct: 301 KLPVVHHQPYVGEFMSDNPRFGATIVLPFQLASSSVKVVGTLQDNVHLQAFASPAPFLAP 360

Query: 361 PSFSLLPPQSTSIIPSLAIFVGKFSDVHSEGSLRLNSSTDVKNSPIVRFNYYSHPDDLAR 420
           P+FSLLPPQ+TSI PSL IFVGKFS+VHSEG LRLNS+TD KN+ IVRFNYYSHPDDLAR
Sbjct: 361 PTFSLLPPQATSINPSLVIFVGKFSEVHSEGFLRLNSTTDAKNNVIVRFNYYSHPDDLAR 420

Query: 421 CVRGVRKVGDLLTTQTMEKMKTQDIEGNKGFQFLGLPLPENLWNDSSVEEYCQKTVTTYW 480
           CV GVRKVGDLL TQTMEK+KTQD+EGNKGFQF+G+PLPENL +DSSVEEYC+KTVTTYW
Sbjct: 421 CVEGVRKVGDLLKTQTMEKIKTQDLEGNKGFQFMGVPLPENLGDDSSVEEYCRKTVTTYW 480

Query: 481 HYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGR 515
           HYHGGCLVGKVVD +++VIG++NLRVVDGSTFS SPGTNPMATLMMLGR
Sbjct: 481 HYHGGCLVGKVVDGDYRVIGMKNLRVVDGSTFSDSPGTNPMATLMMLGR 529

BLAST of Clc11G13550 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 497.3 bits (1279), Expect = 1.6e-140
Identity = 249/500 (49.80%), Postives = 335/500 (67.00%), Query Frame = 0

Query: 24  SNTIPNQEKEEHDYIVIGGGTAGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNA 83
           SN      ++ +DYI++GGGTAGCPLAATLS  F VLLLERG  P   P+V++  G L  
Sbjct: 43  SNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMSHDGFLTT 102

Query: 84  FVAEDDGQNPFQHFTSEDGVENLRGRVLGGGSMINAGFYSRGHREFFETAGVDWDMELVE 143
               ++  +P Q F SE+GV N RGRVLGG S INAGFYSR  ++FFE +G+ WD+  V 
Sbjct: 103 LTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENSGLVWDLSSVN 162

Query: 144 KAYQWVEETVVSRPSLSAWQSAFRSALLEAGVGPDNGFDLRHLVGTKTGGSIFDDKGNRH 203
           ++Y+WVE  +V RP L  WQ+A R ALLE GV P NGF L H VGTK GGS FD  G RH
Sbjct: 163 QSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRH 222

Query: 204 GAVELLNKAEPKNLKVATQATVQRIIF--------DGLSASGVSYFDSKGKLHTAFIHKK 263
            + +LL  A   N++VA  ATV+R++           +SA GV Y D  G+ H A I  +
Sbjct: 223 SSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDR 282

Query: 264 GEIIVSAGAIGSPQLLLLSGVGPKSHLLSLRLPVVLDQPHVGGFMSDNPRLSPTLVLPFQ 323
           GE+I+SAGA+GSPQLL LSG+GP+S+L +  +PV LDQPHVG F+ DNPR   ++V P  
Sbjct: 283 GEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVP 342

Query: 324 LVASSAQIVGTLDNNIHLQSFASPLPFFAP-PSFSLLPPQSTSIIPSLAIFVGKFSDVHS 383
           +  S  Q+VG  ++   L++ ++ +PF +P  S  +  P S   +P   I + K     S
Sbjct: 343 MENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPASPLYVPVTTI-MEKILGPVS 402

Query: 384 EGSLRLNSSTDVKNSPIVRFNYYSHPDDLARCVRGVRKVGDLLTTQTMEKMKTQDIEGNK 443
            G LRL +STDV+ +P+VRFNY+S P DL RCV G RK+G++L ++ M+    ++  GN+
Sbjct: 403 IGLLRL-ASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIREWFGNR 462

Query: 444 GFQFLGLPLPENLWNDSSVEEYCQKTVTTYWHYHGGCLVGKVVDDNHKVIGIENLRVVDG 503
            F+F+G PLP +  ND  + ++C++TV+T WHYHGG +VGKVVD + KVIG+ +LR+VDG
Sbjct: 463 RFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDG 522

Query: 504 STFSVSPGTNPMATLMMLGR 515
           STF++SPGTNP ATLMMLGR
Sbjct: 523 STFNISPGTNPQATLMMLGR 540

BLAST of Clc11G13550 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 395.2 bits (1014), Expect = 8.7e-110
Identity = 237/558 (42.47%), Postives = 317/558 (56.81%), Query Frame = 0

Query: 10  LFILISIF-QLGFLSSNTIPNQE----------KEEHDYIVIGGGTAGCPLAATLSSKFS 69
           LF+ +S+F      SS+  PN               +DYI+IGGGTAGCPLAATLS   S
Sbjct: 10  LFVALSLFLHSPICSSDKAPNYSFMRDATGSPTTSYYDYIIIGGGTAGCPLAATLSQNAS 69

Query: 70  VLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFTSEDGVENLRGRVLGGGSMIN 129
           VLLLERG  P   P++        A +++    +P Q F SEDGV N R RVLGGGS +N
Sbjct: 70  VLLLERGDSPYNNPNI-TRLSAFGAALSDLSESSPSQRFVSEDGVINARARVLGGGSALN 129

Query: 130 AGFYSRGHREFFETAGVDWDMELVEKAYQWVEETVVSRPSLSAWQSAFRSALLEAGVGPD 189
           AGFY+R   ++    G  WD  L  ++YQWVE  V  +P +  WQ+A R  LLEAG+ P+
Sbjct: 130 AGFYTRAGTKYVRNMG--WDGALANESYQWVEAKVAFQPPMGRWQTAVRDGLLEAGIVPN 189

Query: 190 NGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAEPKNLKVATQATVQRIIFDGLS---- 249
           NGF   H+ GTK GG+IFD  GNRH A +LL  A+PK + V   ATV RI+F        
Sbjct: 190 NGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRILFRTRGTTKP 249

Query: 250 -ASGVSYFDSKGKLHTAFIHKKG---EIIVSAGAIGSPQLLLLSGVGPKSHLLSLRLPVV 309
            A+GV Y D  G+ H A++ K+G   EII+SAG +GSPQLL+LSGVGP + L +  + VV
Sbjct: 250 IANGVVYRDRTGQAHRAYL-KEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNITVV 309

Query: 310 LDQPHVGGFMSDNPRLSPTLVLPFQLVASSAQIVGTLDNNIHLQS--------------- 369
           +DQPHVG  M DNP  +  +  P  +  S  ++VG      ++++               
Sbjct: 310 MDQPHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGGGGGGSSG 369

Query: 370 FASPLPFFA--PPSFSLLPPQSTSIIPSLAIFVG-----KFSDVHSEGSLRLNSSTDVKN 429
            +S   ++A   P  +LL   S + + S   F G     K     S G L L  + + K+
Sbjct: 370 SSSTRDYYAMFSPRATLLESNSMTKLSSAQPFQGGFLLEKVMGPLSTGHLEL-KTRNPKD 429

Query: 430 SPIVRFNYYSHPDDLARCVRGVRKVGDLLTTQTMEKMKTQDIEGNKGFQF---LGLPLPE 489
           +PIV FNY+ HPDDL RCVRG++ +  ++ ++   + K  D+     F++   L    P 
Sbjct: 430 NPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADV----SFEYLLNLTASTPV 489

Query: 490 NLWN---------DSSVEEYCQKTVTTYWHYHGGCLVGKVVDDNHKVIGIENLRVVDGST 515
           NL             S EE+CQ TVTT WHYHGGC+VG+VVD ++KVIGI+ LRV+D ST
Sbjct: 490 NLRPPRSGPGASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRLRVIDMST 549

BLAST of Clc11G13550 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 389.8 bits (1000), Expect = 3.7e-108
Identity = 231/542 (42.62%), Postives = 308/542 (56.83%), Query Frame = 0

Query: 23  SSNTIPNQEKEEHDYIVIGGGTAGCPLAATLSSKFSVLLLERGSDP--NKYPSVLNEQGL 82
           SS+   N +   +DYIVIGGGTAGCPLAATLS  FSVL+LERG  P  N   S L     
Sbjct: 51  SSSFSSNGQDSSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRN--- 110

Query: 83  LNAFVAEDDGQNPFQHFTSEDGVENLRGRVLGGGSMINAGFYSRGHREFFETAGVDWDME 142
            +  +A+    +  Q F S DGV N R RVLGGGS INAGFYSR    F + AG  WD +
Sbjct: 111 FHIGLADISASSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPK 170

Query: 143 LVEKAYQWVEETVVSRPSLSAWQSAFRSALLEAGVGPDNGFDLRHLVGTKTGGSIFDDKG 202
           LV+++Y WVE  +V +P L+ WQ A R +LLE GV P NGF   H+ GTK GG+IFD  G
Sbjct: 171 LVKESYPWVEREIVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFG 230

Query: 203 NRHGAVELLNKAEPKNLKVATQATVQRIIFDGLS----ASGVSYFDSKGKLHTAFI--HK 262
            RH A ELL  A P+ L+V   ATVQ+I+FD        +GV + D KG  H A +   K
Sbjct: 231 RRHTAAELLAYANPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRK 290

Query: 263 KGEIIVSAGAIGSPQLLLLSGVGPKSHLLSLRLPVVLDQPHVGGFMSDNPRLSPTLVLPF 322
             E+I+S+GAIGSPQ+L+LSG+GPK  L  L++PVVL+  HVG  M+DNP  +  +    
Sbjct: 291 GSEVILSSGAIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKA 350

Query: 323 QLVASSAQIVGTLDNNIHLQSFASPLPFFAPPS---------------FSLLP-----PQ 382
            +  S  Q VG     +++++      F   P                FS +P     P+
Sbjct: 351 PIEQSLIQTVGITKMGVYVEASTG---FGQSPESIHTHYGIMSNKNELFSTIPAKQRRPE 410

Query: 383 STSIIPSL-----------AIFVGKFSDVHSEGSLRLNSSTDVKNSPIVRFNYYSHPDDL 442
           +T    +            +  + K +   S G L L  +T+V ++P V FNY+ HP DL
Sbjct: 411 ATQAYITRNKYQLHEAFNGSFILEKLAYPISRGHLSL-VNTNVDDNPSVTFNYFKHPVDL 470

Query: 443 ARCVRGVRKVGDLLTT-----------QTMEKMKTQDIEGNKGFQFLGLPLPENLWNDSS 502
            RCV  +R V  ++T+           Q + KM +  ++ N   +      P+ L +  S
Sbjct: 471 QRCVEAIRLVSKVVTSNRFLNYTQCDKQNVHKMLSLSVKANINLR------PKQLNDTKS 530

Query: 503 VEEYCQKTVTTYWHYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMML 515
           + ++C+ TV T WHYHGGCLVGKVV  N KV+G++ LRV+DGSTF  SPGTNP AT+MM+
Sbjct: 531 MAQFCKDTVVTIWHYHGGCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMM 577

BLAST of Clc11G13550 vs. TAIR 10
Match: AT1G72970.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 372.1 bits (954), Expect = 7.9e-103
Identity = 224/531 (42.18%), Postives = 297/531 (55.93%), Query Frame = 0

Query: 23  SSNTIPNQEKEEHDYIVIGGGTAGCPLAATLSSKFSVLLLERGSDP--NKYPSVLNEQGL 82
           SS+   N +   +DYIVIGGGTAGCPLAATLS  FSVL+LERG  P  N   S L     
Sbjct: 51  SSSFSSNGQDSSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRN--- 110

Query: 83  LNAFVAEDDGQNPFQHFTSEDGVENLRGRVLGGGSMINAGFYSRGHREFFETAGVDWDME 142
            +  +A+    +  Q F S DGV N R RVLGGGS INAGFYSR    F + AG  WD +
Sbjct: 111 FHIGLADISASSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPK 170

Query: 143 LVEKAYQWVEETVVSRPSLSAWQSAFRSALLEAGVGPDNGFDLRHLVGTKTGGSIFDDKG 202
           LV+++Y WVE  +V +P L+ WQ A R +LLE GV P NGF   H+ GTK GG+IFD  G
Sbjct: 171 LVKESYPWVEREIVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFG 230

Query: 203 NRHGAVELLNKAEPKNLKVATQATVQRIIFDGLS----ASGVSYFDSKGKLHTAFI--HK 262
            RH A ELL  A P+ L+V   ATVQ+I+FD        +GV + D KG  H A +   K
Sbjct: 231 RRHTAAELLAYANPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRK 290

Query: 263 KGEIIVSAGAIGSPQLLLLSGVGPKSHLLSLRLPVVLDQPHVGGFMSDNPRLSPTLVLPF 322
             E+I+S+GAIGSPQ+L+LSG+GPK  L  L++PVVL+  HVG  M+DNP  +  +    
Sbjct: 291 GSEVILSSGAIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKA 350

Query: 323 QLVASSAQIVGTLDNNIHLQSFASPLPFFAPPS---------------FSLLP-----PQ 382
            +  S  Q VG     +++++      F   P                FS +P     P+
Sbjct: 351 PIEQSLIQTVGITKMGVYVEASTG---FGQSPESIHTHYGIMSNKNELFSTIPAKQRRPE 410

Query: 383 STSIIPSL-----------AIFVGKFSDVHSEGSLRLNSSTDVKNSPIVRFNYYSHPDDL 442
           +T    +            +  + K +   S G L L  +T+V ++P V FNY+ HP D 
Sbjct: 411 ATQAYITRNKYQLHEAFNGSFILEKLAYPISRGHLSL-VNTNVDDNPSVTFNYFKHPCD- 470

Query: 443 ARCVRGVRKVGDLLTTQTMEKMKTQDIEGNKGFQFLGLPLPENLWNDSSVEEYCQKTVTT 502
                           Q + KM +  ++ N   +      P+ L +  S+ ++C+ TV T
Sbjct: 471 ---------------KQNVHKMLSLSVKANINLR------PKQLNDTKSMAQFCKDTVVT 530

Query: 503 YWHYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGR 515
            WHYHGGCLVGKVV  N KV+G++ LRV+DGSTF  SPGTNP AT+MM+GR
Sbjct: 531 IWHYHGGCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGR 550

BLAST of Clc11G13550 vs. TAIR 10
Match: AT5G51930.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 370.9 bits (951), Expect = 1.8e-102
Identity = 224/544 (41.18%), Postives = 316/544 (58.09%), Query Frame = 0

Query: 7   TILLFILISIFQLGFLSSNTIPNQEKEEHDYIVIGGGTAGCPLAATLSSKFSVLLLERGS 66
           +I  F L+ + +  F+   T+   +    DYI+IGGGTAGC LAATLS   SVL+LERG 
Sbjct: 47  SIFFFFLVKL-ETTFMKDATLA-PKNASFDYIIIGGGTAGCALAATLSQNASVLVLERGG 106

Query: 67  DPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFTSEDGVENLRGRVLGGGSMINAGFYSRGH 126
            P + P+  +    +N  +  +   +  Q F SEDGV N R RVLGGGS+IN GFYSR  
Sbjct: 107 SPYENPTATDMGNSVNTLL-NNTPNSWSQLFISEDGVYNTRPRVLGGGSVINGGFYSRAG 166

Query: 127 REFFETAGVDWDMELVEKAYQWVEETVVSRPSLSAWQSAFRSALLEAGVGPDNGFDLRHL 186
            ++ E A  +W+ME VE AY+WVE+ +V  P +  WQ AF+  LLEAG  PDNGF   H+
Sbjct: 167 NDYVEEA--EWEMEEVEAAYEWVEKKLVFEPQVIEWQKAFKDGLLEAGESPDNGFTYDHI 226

Query: 187 VGTKTGGSIFDDKGNRHGAVELLNKAEPKNLKVATQATVQRIIFDGLSASGVSYFDSKGK 246
            GTK GG+IFD  G+RH A  LL  A P  + V   A+V +++F    A  V + D+ G 
Sbjct: 227 YGTKIGGTIFDRAGHRHTAANLLEYANPNRIVVYLHASVHKVLFT-TEAYEVLFEDANGV 286

Query: 247 LHTAFIHKK--GEIIVSAGAIGSPQLLLLSGVGPKSHLLSLRL-PVVLDQPHVGGFMSDN 306
            H A +  K   E+I+SAGA+GSPQLL+LSGVGP  HL +  + P+VLDQP VG  M+DN
Sbjct: 287 FHKANLANKATNEVILSAGALGSPQLLMLSGVGPAVHLEAHGVNPLVLDQPMVGQGMADN 346

Query: 307 PRLSPTLVLPFQLVASSAQIVGTLDNNIHLQSFAS-PLPF-----FAPPSFSLLPPQSTS 366
           P     +  P  +  S  Q VG    + +++  +   L F     F     +LL   S +
Sbjct: 347 PMNFVAIPSPQPVELSLIQAVGITKFDSYIEGLSGLSLSFDITRRFFDGVLNLLNETSHT 406

Query: 367 -----IIPSLAIFVGKFSDVH------------------SEGSLRLNSSTDVKNSPIVRF 426
                +  S+A+ +  F DV                   S+G ++L  +T+ +++P V F
Sbjct: 407 TSRKILTQSIAVLLKSF-DVKLEVRMNGGLIFQKVDGPASKGHMKLR-NTNPRDNPSVTF 466

Query: 427 NYYSHPDDLARCVRGVRKVGDLLTTQTMEKMKTQDIEGNKGFQ-FLGLPL---PENLWND 486
           NYY  P+DL +CV+G+  +  ++ ++   K K   +   +     L LP+   P ++ + 
Sbjct: 467 NYYQEPEDLNKCVKGLNTIIRMINSKAFSKYKYPGVTARELLNLMLALPINLRPRHVTSA 526

Query: 487 SSVEEYCQKTVTTYWHYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLM 515
            +++++C  TVT+ WHYHGGC VGKVVD N+KV+GI+ LRV+DGSTF  SPGTNP AT+M
Sbjct: 527 FNLKQFCIDTVTSVWHYHGGCQVGKVVDKNYKVLGIDGLRVIDGSTFLKSPGTNPQATVM 582

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038889699.14.0e-26687.90(R)-mandelonitrile lyase 1-like [Benincasa hispida][more]
XP_031736478.11.2e-26587.55(R)-mandelonitrile lyase 1-like [Cucumis sativus] >KGN61117.1 hypothetical prote... [more]
XP_008451922.17.5e-26588.12PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo] >KAA0067298.1 (R)-mand... [more]
XP_038889315.11.2e-24680.57(R)-mandelonitrile lyase 1-like [Benincasa hispida][more]
XP_038889314.13.5e-24681.13(R)-mandelonitrile lyase 1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
P527061.3e-14251.65(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1[more]
P527071.6e-14049.90(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1[more]
Q945K24.6e-14050.83(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1[more]
Q9SSM22.3e-13949.80(R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 ... [more]
O500481.3e-13449.18(R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LK795.6e-26687.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055050 PE=3 SV=1[more]
A0A5D3CL003.7e-26588.12(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3BSP43.7e-26588.12(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493073 PE=3 SV=... [more]
A0A6J1K4X82.9e-24679.02(R)-mandelonitrile lyase 1-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1EP462.7e-24478.64(R)-mandelonitrile lyase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT1G73050.11.6e-14049.80Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G12570.18.7e-11042.47Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.13.7e-10842.62Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.27.9e-10342.18Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51930.11.8e-10241.18Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 373..514
e-value: 1.7E-28
score: 99.9
NoneNo IPR availableGENE3D3.30.410.40coord: 155..463
e-value: 4.9E-168
score: 562.0
NoneNo IPR availablePIRSRPIRSR000137-2PIRSR000137-2coord: 29..307
e-value: 2.5E-65
score: 219.1
NoneNo IPR availablePANTHERPTHR45968:SF23(R)-MANDELONITRILE LYASE 4-RELATEDcoord: 26..514
NoneNo IPR availablePANTHERPTHR45968OSJNBA0019K04.7 PROTEINcoord: 26..514
NoneNo IPR availableSUPERFAMILY54373FAD-linked reductases, C-terminal domaincoord: 373..470
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 35..513
e-value: 4.9E-168
score: 562.0
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 28..514
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 36..300
e-value: 3.7E-33
score: 115.2
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00623GMC_OXRED_1coord: 108..131
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00624GMC_OXRED_2coord: 263..277
IPR012132Glucose-methanol-choline oxidoreductasePIRSFPIRSF000137Alcohol_oxidasecoord: 352..516
e-value: 1.8E-29
score: 100.9
coord: 9..335
e-value: 2.7E-59
score: 199.4

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc11G13550.2Clc11G13550.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors