Clc10G20950 (gene) Watermelon (cordophanus) v2

Overview
NameClc10G20950
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
LocationClcChr10: 34293084 .. 34294559 (+)
RNA-Seq ExpressionClc10G20950
SyntenyClc10G20950
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTAGAAATAAACAAATTGGAAGAGAGTATTGGAGAGAGAATAAATAAATTGTAAGCATCTCTATGTTTGGGAATTGGGTACCTACAAACAGAATTGAAATCCCAAAGGACTTTCCCTTATCTTCTCCATTTCTCTCTTCAATTCATTGCAGATGAGTTCCCCACTTCAAAGATGGTCTCTCAACGGCTTCACCGCACTCGTCACCGGCGGAACCCGTGGCATTGGGTCAGCCCTTAATCAGTACTATCAATCCCAAATTTCATATCATTTCTTTCATGTTCTTACTTTTTTCTGTTTTGAAAATCTCTTAGATACGCCATAGTTGAAGAACTTGCTTCACTCGGGGCAGCCGTACACACTTGTTCCCGTAACCAAACAGAGCTCCATCAAAGGCTAAAAGAATGGGAACAGAAAGGGTTCAAAGTCACTGCCTCTGTTTGTGATCTCACTTCAAAATCTCAGCGCCAGCAACTCATTGACACTGTCACTTCCACCTTCAATGGAAGCCTCAACATCCTCGTAATTCTCTCTCAAATTTTTTCTATTTTTTAAACAAACCCAGATGAAAAATTGTCGAAATTCTGAAAATGGGTGGTTGGTTTTCAAGGTCAACAACGCAGGGACGGTAACATTGAAAGGAGCAACAGAGTACACGAGCGAGGATTACAATAACATGATGAGCACAAATTTTGAAGCCCCTTATCATCTCTCCCAAATTTCTCATTCCATTTTGAAGGCATCTGGCTATGGAAGTATTGTGTTTGTGTCGTCGATTGCCGGAGTCACTGCTTTGCCAAAAATTTCAATCTATGCGGCATCTAAAGGTATGGATTTACATAGATAATTTCTTTGTAATTAAAGATTAATCTGGAAATATTTGGGTAATTTGTGTTTGTAGGGGCAATTAACCAAATCACGAAGAACTTGGCGTGTGAATGGGCAAAGGATAATATTCGGGTAAACACAGTGGCGCCGTGGGGAGTTAAAACAACAATTTCTAAACCGGATCCGGTGGCTATGGAGGAATATGGTCGGCTGATAAGGCGGACGCCGGCGGGTCGAATTGGAGAGCCGGAGGAGATATCTTCGGTGGTGGCATTTTTGTGCCTTCCTGCGGCTTCGTATGTGAGTGGGCAGATCATTTGTGTGGATGGTGGCTACACAGCCGGTGGGTGGTAGACAAATCTAAGTAATACCAAACGTATTTAAGAGCTGTAACAAAACCAATTCAAGAAACTATAATCCTAATGTGTTTAAATAGGGTTAAAAAAGTTGAAATTTTCATACAAAAAACACATGGAACTTGTGTCTTGCATTTATTCAATTTTATCTATTTTTCAAAAACTACGCTTTAACCTTATATCTTCATGTTTAGACACCAAGACCTTAAAAGTTAGAATTATTGTAGTTTGTATGTAATTTAACTTTTAAAAAACAAAAACTTGGAAGTTGTGTATGTGTTAATTTTTAATTTT

mRNA sequence

GTTAGAAATAAACAAATTGGAAGAGAGTATTGGAGAGAGAATAAATAAATTGTAAGCATCTCTATGTTTGGGAATTGGGTACCTACAAACAGAATTGAAATCCCAAAGGACTTTCCCTTATCTTCTCCATTTCTCTCTTCAATTCATTGCAGATGAGTTCCCCACTTCAAAGATGGTCTCTCAACGGCTTCACCGCACTCGTCACCGGCGGAACCCGTGGCATTGGATACGCCATAGTTGAAGAACTTGCTTCACTCGGGGCAGCCGTACACACTTGTTCCCGTAACCAAACAGAGCTCCATCAAAGGCTAAAAGAATGGGAACAGAAAGGGTTCAAAGTCACTGCCTCTGTTTGTGATCTCACTTCAAAATCTCAGCGCCAGCAACTCATTGACACTGTCACTTCCACCTTCAATGGAAGCCTCAACATCCTCGTCAACAACGCAGGGACGGTAACATTGAAAGGAGCAACAGAGTACACGAGCGAGGATTACAATAACATGATGAGCACAAATTTTGAAGCCCCTTATCATCTCTCCCAAATTTCTCATTCCATTTTGAAGGCATCTGGCTATGGAAGTATTGTGTTTGTGTCGTCGATTGCCGGAGTCACTGCTTTGCCAAAAATTTCAATCTATGCGGCATCTAAAGGGGCAATTAACCAAATCACGAAGAACTTGGCGTGTGAATGGGCAAAGGATAATATTCGGGTAAACACAGTGGCGCCGTGGGGAGTTAAAACAACAATTTCTAAACCGGATCCGGTGGCTATGGAGGAATATGGTCGGCTGATAAGGCGGACGCCGGCGGGTCGAATTGGAGAGCCGGAGGAGATATCTTCGGTGGTGGCATTTTTGTGCCTTCCTGCGGCTTCGTATGTGAGTGGGCAGATCATTTGTGTGGATGGTGGCTACACAGCCGGTGGGTGGTAGACAAATCTAAGTAATACCAAACGTATTTAAGAGCTGTAACAAAACCAATTCAAGAAACTATAATCCTAATGTGTTTAAATAGGGTTAAAAAAGTTGAAATTTTCATACAAAAAACACATGGAACTTGTGTCTTGCATTTATTCAATTTTATCTATTTTTCAAAAACTACGCTTTAACCTTATATCTTCATGTTTAGACACCAAGACCTTAAAAGTTAGAATTATTGTAGTTTGTATGTAATTTAACTTTTAAAAAACAAAAACTTGGAAGTTGTGTATGTGTTAATTTTTAATTTT

Coding sequence (CDS)

ATGAGTTCCCCACTTCAAAGATGGTCTCTCAACGGCTTCACCGCACTCGTCACCGGCGGAACCCGTGGCATTGGATACGCCATAGTTGAAGAACTTGCTTCACTCGGGGCAGCCGTACACACTTGTTCCCGTAACCAAACAGAGCTCCATCAAAGGCTAAAAGAATGGGAACAGAAAGGGTTCAAAGTCACTGCCTCTGTTTGTGATCTCACTTCAAAATCTCAGCGCCAGCAACTCATTGACACTGTCACTTCCACCTTCAATGGAAGCCTCAACATCCTCGTCAACAACGCAGGGACGGTAACATTGAAAGGAGCAACAGAGTACACGAGCGAGGATTACAATAACATGATGAGCACAAATTTTGAAGCCCCTTATCATCTCTCCCAAATTTCTCATTCCATTTTGAAGGCATCTGGCTATGGAAGTATTGTGTTTGTGTCGTCGATTGCCGGAGTCACTGCTTTGCCAAAAATTTCAATCTATGCGGCATCTAAAGGGGCAATTAACCAAATCACGAAGAACTTGGCGTGTGAATGGGCAAAGGATAATATTCGGGTAAACACAGTGGCGCCGTGGGGAGTTAAAACAACAATTTCTAAACCGGATCCGGTGGCTATGGAGGAATATGGTCGGCTGATAAGGCGGACGCCGGCGGGTCGAATTGGAGAGCCGGAGGAGATATCTTCGGTGGTGGCATTTTTGTGCCTTCCTGCGGCTTCGTATGTGAGTGGGCAGATCATTTGTGTGGATGGTGGCTACACAGCCGGTGGGTGGTAG

Protein sequence

MSSPLQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELHQRLKEWEQKGFKVTASVCDLTSKSQRQQLIDTVTSTFNGSLNILVNNAGTVTLKGATEYTSEDYNNMMSTNFEAPYHLSQISHSILKASGYGSIVFVSSIAGVTALPKISIYAASKGAINQITKNLACEWAKDNIRVNTVAPWGVKTTISKPDPVAMEEYGRLIRRTPAGRIGEPEEISSVVAFLCLPAASYVSGQIICVDGGYTAGGW
Homology
BLAST of Clc10G20950 vs. NCBI nr
Match: XP_008442514.1 (PREDICTED: tropinone reductase homolog [Cucumis melo] >KAA0044113.1 tropinone reductase-like protein [Cucumis melo var. makuwa] >TYK25025.1 tropinone reductase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 474.6 bits (1220), Expect = 5.6e-130
Identity = 238/259 (91.89%), Postives = 250/259 (96.53%), Query Frame = 0

Query: 1   MSSPLQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELHQRLKEWEQKG 60
           MSSPLQRWSL+GFTALVTGGTRGIGYAIVEELASLGA+VHTCSRNQTE+ QRL+EW+QKG
Sbjct: 1   MSSPLQRWSLDGFTALVTGGTRGIGYAIVEELASLGASVHTCSRNQTEIDQRLQEWQQKG 60

Query: 61  FKVTASVCDLTSKSQRQQLIDTVTSTFNGSLNILVNNAGTVTLKGATEYTSEDYNNMMST 120
           FKVTASVCDLTS SQRQQLIDTV+S FNG+LNILVNNAGTVTLKGATEYT+EDYN MMST
Sbjct: 61  FKVTASVCDLTSSSQRQQLIDTVSSIFNGTLNILVNNAGTVTLKGATEYTTEDYNYMMST 120

Query: 121 NFEAPYHLSQISHSILKASGYGSIVFVSSIAGVTALPKISIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQISH ILKASGYGSIVFVSSIAGVTALPKISIYAA+KGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISHPILKASGYGSIVFVSSIAGVTALPKISIYAATKGAINQITKNLACEW 180

Query: 181 AKDNIRVNTVAPWGVKTTISKPDPVAMEEYGRLIRRTPAGRIGEPEEISSVVAFLCLPAA 240
           AKDNIRVNTVAPWGV+TTISKPD   +EEYGRLI RTPAGRIGEPEEISSVVAFLCLPAA
Sbjct: 181 AKDNIRVNTVAPWGVRTTISKPDAAVVEEYGRLIGRTPAGRIGEPEEISSVVAFLCLPAA 240

Query: 241 SYVSGQIICVDGGYTAGGW 260
           SYVSGQIICVDGG+TAGGW
Sbjct: 241 SYVSGQIICVDGGFTAGGW 259

BLAST of Clc10G20950 vs. NCBI nr
Match: XP_004137775.1 (tropinone reductase homolog [Cucumis sativus] >KGN58843.1 hypothetical protein Csa_002554 [Cucumis sativus])

HSP 1 Score: 471.1 bits (1211), Expect = 6.2e-129
Identity = 235/259 (90.73%), Postives = 249/259 (96.14%), Query Frame = 0

Query: 1   MSSPLQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELHQRLKEWEQKG 60
           MSSPLQRWSLNGFTALVTGGTRGIGYAIVEELASLGA+VHTCSRNQTE+ QRL+EW+QKG
Sbjct: 1   MSSPLQRWSLNGFTALVTGGTRGIGYAIVEELASLGASVHTCSRNQTEIDQRLQEWQQKG 60

Query: 61  FKVTASVCDLTSKSQRQQLIDTVTSTFNGSLNILVNNAGTVTLKGATEYTSEDYNNMMST 120
           FKVTASVCDLTS SQRQQLIDTV+S FNG+LNILVNNAGTVT+K ATEYT+EDYN MMST
Sbjct: 61  FKVTASVCDLTSSSQRQQLIDTVSSIFNGTLNILVNNAGTVTMKTATEYTTEDYNYMMST 120

Query: 121 NFEAPYHLSQISHSILKASGYGSIVFVSSIAGVTALPKISIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQISH ILKASGYGSIVFVSSIAGVTALP+ISIYAA+KGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISHPILKASGYGSIVFVSSIAGVTALPRISIYAATKGAINQITKNLACEW 180

Query: 181 AKDNIRVNTVAPWGVKTTISKPDPVAMEEYGRLIRRTPAGRIGEPEEISSVVAFLCLPAA 240
           AKDNIR+NTVAPWGV+TTIS PD  A+EEYGRLI RTPAGR+GEPEEISSVVAFLCLPAA
Sbjct: 181 AKDNIRINTVAPWGVRTTISTPDAAAVEEYGRLIGRTPAGRLGEPEEISSVVAFLCLPAA 240

Query: 241 SYVSGQIICVDGGYTAGGW 260
           SYVSGQIICVDGGYTAGGW
Sbjct: 241 SYVSGQIICVDGGYTAGGW 259

BLAST of Clc10G20950 vs. NCBI nr
Match: XP_038905102.1 (tropinone reductase homolog [Benincasa hispida])

HSP 1 Score: 468.0 bits (1203), Expect = 5.3e-128
Identity = 237/259 (91.51%), Postives = 246/259 (94.98%), Query Frame = 0

Query: 1   MSSPLQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELHQRLKEWEQKG 60
           MSSPLQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTEL +RLKEWEQKG
Sbjct: 1   MSSPLQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDERLKEWEQKG 60

Query: 61  FKVTASVCDLTSKSQRQQLIDTVTSTFNGSLNILVNNAGTVTLKGATEYTSEDYNNMMST 120
           FKVTASVCDLTSKSQRQ LIDTV+S F+G LNILVNNAGTVTLK ATE+TSEDYN MMST
Sbjct: 61  FKVTASVCDLTSKSQRQHLIDTVSSIFDGKLNILVNNAGTVTLKPATEHTSEDYNYMMST 120

Query: 121 NFEAPYHLSQISHSILKASGYGSIVFVSSIAGVTALPKISIYAASKGAINQITKNLACEW 180
           NFEA YHLSQISH ILKASGYGSIVFVSSIAGVTALP+ISIYAASK AINQ+TKNLACEW
Sbjct: 121 NFEASYHLSQISHPILKASGYGSIVFVSSIAGVTALPRISIYAASKAAINQLTKNLACEW 180

Query: 181 AKDNIRVNTVAPWGVKTTISKPDPVAMEEYGRLIRRTPAGRIGEPEEISSVVAFLCLPAA 240
           AKDNIRVNTVAPWGV+TTISKPD   +EEYGRLI RTPAGRIGEPEEISSVVAFLCLPAA
Sbjct: 181 AKDNIRVNTVAPWGVRTTISKPDAAVVEEYGRLIGRTPAGRIGEPEEISSVVAFLCLPAA 240

Query: 241 SYVSGQIICVDGGYTAGGW 260
           SYV+GQIICVDGGYTAGGW
Sbjct: 241 SYVTGQIICVDGGYTAGGW 259

BLAST of Clc10G20950 vs. NCBI nr
Match: XP_023527559.1 (tropinone reductase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 443.4 bits (1139), Expect = 1.4e-120
Identity = 220/258 (85.27%), Postives = 239/258 (92.64%), Query Frame = 0

Query: 1   MSSPLQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELHQRLKEWEQKG 60
           MSSP+QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTEL QR+KEWE+KG
Sbjct: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60

Query: 61  FKVTASVCDLTSKSQRQQLIDTVTSTFNGSLNILVNNAGTVTLKGATEYTSEDYNNMMST 120
           F+V+ SVCDL+S+SQR+QLI TV+S FNG LNILVNNAGTVTLK A E+T+EDYNN+MST
Sbjct: 61  FEVSGSVCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMST 120

Query: 121 NFEAPYHLSQISHSILKASGYGSIVFVSSIAGVTALPKISIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQIS+  LKASGYGSIVFVSS+AGV ALPK+SIYAASKGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180

Query: 181 AKDNIRVNTVAPWGVKTTISKPDPVAMEEYGRLIRRTPAGRIGEPEEISSVVAFLCLPAA 240
           AKDNIRVN VAPW VKTTISKPD   +EEY RL+ RTPAGRIGE  EISSVVAFLCLPAA
Sbjct: 181 AKDNIRVNAVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240

Query: 241 SYVSGQIICVDGGYTAGG 259
           SYV+GQ+ICVDGGYTAGG
Sbjct: 241 SYVTGQVICVDGGYTAGG 258

BLAST of Clc10G20950 vs. NCBI nr
Match: KAG7017273.1 (hypothetical protein SDJN02_19136, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 442.2 bits (1136), Expect = 3.1e-120
Identity = 219/258 (84.88%), Postives = 239/258 (92.64%), Query Frame = 0

Query: 1   MSSPLQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELHQRLKEWEQKG 60
           M+SP+QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTEL QR+KEWE+KG
Sbjct: 6   MNSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 65

Query: 61  FKVTASVCDLTSKSQRQQLIDTVTSTFNGSLNILVNNAGTVTLKGATEYTSEDYNNMMST 120
           F+V+ SVCDL+S+SQRQQLI TV+S FNG LNILVNNAG VTLK A E+T+EDYNN+MST
Sbjct: 66  FEVSGSVCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMST 125

Query: 121 NFEAPYHLSQISHSILKASGYGSIVFVSSIAGVTALPKISIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQIS+  LKASGYGSIVFVSS+AGV ALPKISIYAASKGAINQITKNLACEW
Sbjct: 126 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGAINQITKNLACEW 185

Query: 181 AKDNIRVNTVAPWGVKTTISKPDPVAMEEYGRLIRRTPAGRIGEPEEISSVVAFLCLPAA 240
           AKDNIR+NTVAPW VKTTIS+PD   +EEY RL+ RTPAGRIGE  EISSVVAFLCLPAA
Sbjct: 186 AKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 245

Query: 241 SYVSGQIICVDGGYTAGG 259
           SYV+GQ+ICVDGGYTAGG
Sbjct: 246 SYVTGQVICVDGGYTAGG 263

BLAST of Clc10G20950 vs. ExPASy Swiss-Prot
Match: P50165 (Tropinone reductase homolog OS=Datura stramonium OX=4076 PE=2 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 8.8e-86
Identity = 162/257 (63.04%), Postives = 200/257 (77.82%), Query Frame = 0

Query: 6   QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELHQRLKEWEQKGFKVTA 65
           +RWSL G TALVTGGTRGIGYAIVEELA+ GA V+TCSR+Q +L + L++W +KGFKV+ 
Sbjct: 11  RRWSLRGMTALVTGGTRGIGYAIVEELANFGAEVYTCSRSQNDLDECLEKWRRKGFKVSG 70

Query: 66  SVCDLTSKSQRQQLIDTVTSTFNGSLNILVNNAGTVTLKGATEYTSEDYNNMMSTNFEAP 125
            VCD++S SQRQ L+++VTS+FNG LNIL+NNAGT   K AT +T+EDY+ +M TNFEA 
Sbjct: 71  PVCDVSSISQRQTLMESVTSSFNGKLNILINNAGTTIPKEATNFTAEDYSIIMGTNFEAS 130

Query: 126 YHLSQISHSILKASGYGSIVFVSSIAGVTALPKISIYAASKGAINQITKNLACEWAKDNI 185
           Y+L Q++H +LKASG  SIVF SS AGV A+P  SIYAASKGAINQ+TK+LACEWAKD+I
Sbjct: 131 YNLCQLAHPLLKASGNASIVFNSSAAGVIAVPLSSIYAASKGAINQVTKSLACEWAKDSI 190

Query: 186 RVNTVAPWGVKTTISKPD---PVAMEEYGRLIRRTPAGRIGEPEEISSVVAFLCLPAASY 245
           RVN VAPW + T I +     P   +    LI R P  R GEP E+SS+V +LCLP ASY
Sbjct: 191 RVNAVAPWIINTPIIEAACQVPSQKKNIESLIGRAPMKRAGEPSEVSSLVTYLCLPTASY 250

Query: 246 VSGQIICVDGGYTAGGW 260
           ++GQIICVDGGYT  G+
Sbjct: 251 ITGQIICVDGGYTVNGF 267

BLAST of Clc10G20950 vs. ExPASy Swiss-Prot
Match: P50162 (Tropinone reductase 1 OS=Datura stramonium OX=4076 GN=TR1 PE=1 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 9.2e-83
Identity = 155/256 (60.55%), Postives = 198/256 (77.34%), Query Frame = 0

Query: 7   RWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELHQRLKEWEQKGFKVTAS 66
           RWSL G TALVTGG++GIGYAIVEELA LGA V+TCSRN+ EL + L+ W +KG  V  S
Sbjct: 16  RWSLKGTTALVTGGSKGIGYAIVEELAGLGARVYTCSRNEKELDECLEIWREKGLNVEGS 75

Query: 67  VCDLTSKSQRQQLIDTVTSTFNGSLNILVNNAGTVTLKGATEYTSEDYNNMMSTNFEAPY 126
           VCDL S+++R +L+ TV   F+G LNILVNNAG V  K A ++T +DYN +M TNFEA Y
Sbjct: 76  VCDLLSRTERDKLMQTVAHVFDGKLNILVNNAGVVIHKEAKDFTEKDYNIIMGTNFEAAY 135

Query: 127 HLSQISHSILKASGYGSIVFVSSIAGVTALPKISIYAASKGAINQITKNLACEWAKDNIR 186
           HLSQI++ +LKAS  G+++F+SSIAG +ALP +S+Y+ASKGAINQ+TK+LACEWAKDNIR
Sbjct: 136 HLSQIAYPLLKASQNGNVIFLSSIAGFSALPSVSLYSASKGAINQMTKSLACEWAKDNIR 195

Query: 187 VNTVAPWGVKT----TISKPDPVAMEEYGRLIRRTPAGRIGEPEEISSVVAFLCLPAASY 246
           VN+VAP  + T    T  K +P   EE    I +TP GR G+P+E+S+++AFLC PAASY
Sbjct: 196 VNSVAPGVILTPLVETAIKKNPHQKEEIDNFIVKTPMGRAGKPQEVSALIAFLCFPAASY 255

Query: 247 VSGQIICVDGGYTAGG 259
           ++GQII  DGG+TA G
Sbjct: 256 ITGQIIWADGGFTANG 271

BLAST of Clc10G20950 vs. ExPASy Swiss-Prot
Match: Q9LHT0 (Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g06060 PE=2 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 7.7e-82
Identity = 150/254 (59.06%), Postives = 192/254 (75.59%), Query Frame = 0

Query: 6   QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELHQRLKEWEQKGFKVTA 65
           +RWSL G TALVTGGTRGIG A+VEELA  GA VHTCSRNQ EL+  L +W+  G  V+ 
Sbjct: 5   KRWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVSG 64

Query: 66  SVCDLTSKSQRQQLIDTVTSTFNGSLNILVNNAGTVTLKGATEYTSEDYNNMMSTNFEAP 125
           SVCD + + QR++LI   +S F+G LNIL+NN GT   K   EY+SE+Y  +MSTN E+ 
Sbjct: 65  SVCDASVRDQREKLIQEASSAFSGKLNILINNVGTNVRKPTVEYSSEEYAKIMSTNLESA 124

Query: 126 YHLSQISHSILKASGYGSIVFVSSIAGVTALPKISIYAASKGAINQITKNLACEWAKDNI 185
           +HLSQI+H +LKASG GSIVF+SS+AG+  L   SIY A+KGA+NQ+T+NLACEWA DNI
Sbjct: 125 FHLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASDNI 184

Query: 186 RVNTVAPWGVKTTISKPDPVAMEEYGRLIRRTPAGRIGEPEEISSVVAFLCLPAASYVSG 245
           R N VAPW +KT++ +      E    ++ RTP GR+GEPEE+SS+VAFLCLPA+SY++G
Sbjct: 185 RTNCVAPWYIKTSLVETLLEKKEFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYITG 244

Query: 246 QIICVDGGYTAGGW 260
           Q+I VDGG+T  G+
Sbjct: 245 QVISVDGGFTVNGF 258

BLAST of Clc10G20950 vs. ExPASy Swiss-Prot
Match: Q9ZW13 (Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g29290 PE=2 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 2.9e-81
Identity = 145/255 (56.86%), Postives = 201/255 (78.82%), Query Frame = 0

Query: 6   QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELHQRLKEWEQKGFKVTA 65
           +RWSL G  ALVTGGT+GIG A+VEEL+ LGA VHTC+R++T+L +RL+EW++KGF+VT 
Sbjct: 3   KRWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVTT 62

Query: 66  SVCDLTSKSQRQQLIDTVTSTFNGSLNILVNNAGTVTLKGATEYTSEDYNNMMSTNFEAP 125
           S+CD++ + QR++L++TV+S F G LNILVNN GT+ LK  TEYT+E+++ +M+TN ++ 
Sbjct: 63  SICDVSLREQREKLMETVSSLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLDSA 122

Query: 126 YHLSQISHSILKASGYGSIVFVSSIAGVTALPKISIYAASKGAINQITKNLACEWAKDNI 185
           +H+SQ++H +LKASG GSIV +SSIAGV  +   SIY A+KGA+NQ+ +NLACEWA DNI
Sbjct: 123 FHISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASDNI 182

Query: 186 RVNTVAPWGVKTTISKPDPVAMEEYGR-LIRRTPAGRIGEPEEISSVVAFLCLPAASYVS 245
           R N + PW + T +   D +++EE  +    RTP GR+GE  E+S +VAFLCLPAASY++
Sbjct: 183 RTNAICPWLITTPLIS-DLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCLPAASYIT 242

Query: 246 GQIICVDGGYTAGGW 260
           GQ+ICVDGG T  G+
Sbjct: 243 GQVICVDGGLTVNGF 256

BLAST of Clc10G20950 vs. ExPASy Swiss-Prot
Match: Q9ZW18 (Senescence-associated protein 13 OS=Arabidopsis thaliana OX=3702 GN=SAG13 PE=1 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 8.6e-81
Identity = 148/253 (58.50%), Postives = 194/253 (76.68%), Query Frame = 0

Query: 7   RWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELHQRLKEWEQKGFKVTAS 66
           RWSL G TALVTGG++GIG A+VEELA LGA VHTC+R++T+L +RL+EW+ KGF+VT S
Sbjct: 12  RWSLGGMTALVTGGSKGIGEAVVEELAMLGAKVHTCARDETQLQERLREWQAKGFQVTTS 71

Query: 67  VCDLTSKSQRQQLIDTVTSTFNGSLNILVNNAGTVTLKGATEYTSEDYNNMMSTNFEAPY 126
           VCD++S+ QR +L++TV+S + G LNILVNN GT   K  TEYT+ED++ +M+TN E+ +
Sbjct: 72  VCDVSSRDQRVKLMETVSSLYQGKLNILVNNVGTSIFKPTTEYTAEDFSFVMATNLESAF 131

Query: 127 HLSQISHSILKASGYGSIVFVSSIAGVTALPKISIYAASKGAINQITKNLACEWAKDNIR 186
           HLSQ++H +LKASG GSIV +SS AGV  +   SIY A+KGA+NQ+ +NLACEWA DNIR
Sbjct: 132 HLSQLAHPLLKASGSGSIVLISSAAGVVHVNVGSIYGATKGAMNQLARNLACEWASDNIR 191

Query: 187 VNTVAPWGVKTTISKPDPVAMEEYGRLIRRTPAGRIGEPEEISSVVAFLCLPAASYVSGQ 246
            N+V PW + T +S  D    E     +R TP GR+GE  E+S +VAFLCLP+ASY++GQ
Sbjct: 192 TNSVCPWYITTPLSN-DFFDEEFKKEAVRTTPMGRVGEANEVSPLVAFLCLPSASYITGQ 251

Query: 247 IICVDGGYTAGGW 260
            ICVDGG T  G+
Sbjct: 252 TICVDGGATVNGF 263

BLAST of Clc10G20950 vs. ExPASy TrEMBL
Match: A0A5D3DN35 (Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001440 PE=4 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 2.7e-130
Identity = 238/259 (91.89%), Postives = 250/259 (96.53%), Query Frame = 0

Query: 1   MSSPLQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELHQRLKEWEQKG 60
           MSSPLQRWSL+GFTALVTGGTRGIGYAIVEELASLGA+VHTCSRNQTE+ QRL+EW+QKG
Sbjct: 1   MSSPLQRWSLDGFTALVTGGTRGIGYAIVEELASLGASVHTCSRNQTEIDQRLQEWQQKG 60

Query: 61  FKVTASVCDLTSKSQRQQLIDTVTSTFNGSLNILVNNAGTVTLKGATEYTSEDYNNMMST 120
           FKVTASVCDLTS SQRQQLIDTV+S FNG+LNILVNNAGTVTLKGATEYT+EDYN MMST
Sbjct: 61  FKVTASVCDLTSSSQRQQLIDTVSSIFNGTLNILVNNAGTVTLKGATEYTTEDYNYMMST 120

Query: 121 NFEAPYHLSQISHSILKASGYGSIVFVSSIAGVTALPKISIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQISH ILKASGYGSIVFVSSIAGVTALPKISIYAA+KGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISHPILKASGYGSIVFVSSIAGVTALPKISIYAATKGAINQITKNLACEW 180

Query: 181 AKDNIRVNTVAPWGVKTTISKPDPVAMEEYGRLIRRTPAGRIGEPEEISSVVAFLCLPAA 240
           AKDNIRVNTVAPWGV+TTISKPD   +EEYGRLI RTPAGRIGEPEEISSVVAFLCLPAA
Sbjct: 181 AKDNIRVNTVAPWGVRTTISKPDAAVVEEYGRLIGRTPAGRIGEPEEISSVVAFLCLPAA 240

Query: 241 SYVSGQIICVDGGYTAGGW 260
           SYVSGQIICVDGG+TAGGW
Sbjct: 241 SYVSGQIICVDGGFTAGGW 259

BLAST of Clc10G20950 vs. ExPASy TrEMBL
Match: A0A1S3B6I2 (tropinone reductase homolog OS=Cucumis melo OX=3656 GN=LOC103486364 PE=4 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 2.7e-130
Identity = 238/259 (91.89%), Postives = 250/259 (96.53%), Query Frame = 0

Query: 1   MSSPLQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELHQRLKEWEQKG 60
           MSSPLQRWSL+GFTALVTGGTRGIGYAIVEELASLGA+VHTCSRNQTE+ QRL+EW+QKG
Sbjct: 1   MSSPLQRWSLDGFTALVTGGTRGIGYAIVEELASLGASVHTCSRNQTEIDQRLQEWQQKG 60

Query: 61  FKVTASVCDLTSKSQRQQLIDTVTSTFNGSLNILVNNAGTVTLKGATEYTSEDYNNMMST 120
           FKVTASVCDLTS SQRQQLIDTV+S FNG+LNILVNNAGTVTLKGATEYT+EDYN MMST
Sbjct: 61  FKVTASVCDLTSSSQRQQLIDTVSSIFNGTLNILVNNAGTVTLKGATEYTTEDYNYMMST 120

Query: 121 NFEAPYHLSQISHSILKASGYGSIVFVSSIAGVTALPKISIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQISH ILKASGYGSIVFVSSIAGVTALPKISIYAA+KGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISHPILKASGYGSIVFVSSIAGVTALPKISIYAATKGAINQITKNLACEW 180

Query: 181 AKDNIRVNTVAPWGVKTTISKPDPVAMEEYGRLIRRTPAGRIGEPEEISSVVAFLCLPAA 240
           AKDNIRVNTVAPWGV+TTISKPD   +EEYGRLI RTPAGRIGEPEEISSVVAFLCLPAA
Sbjct: 181 AKDNIRVNTVAPWGVRTTISKPDAAVVEEYGRLIGRTPAGRIGEPEEISSVVAFLCLPAA 240

Query: 241 SYVSGQIICVDGGYTAGGW 260
           SYVSGQIICVDGG+TAGGW
Sbjct: 241 SYVSGQIICVDGGFTAGGW 259

BLAST of Clc10G20950 vs. ExPASy TrEMBL
Match: A0A0A0LAG7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G733970 PE=4 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 3.0e-129
Identity = 235/259 (90.73%), Postives = 249/259 (96.14%), Query Frame = 0

Query: 1   MSSPLQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELHQRLKEWEQKG 60
           MSSPLQRWSLNGFTALVTGGTRGIGYAIVEELASLGA+VHTCSRNQTE+ QRL+EW+QKG
Sbjct: 1   MSSPLQRWSLNGFTALVTGGTRGIGYAIVEELASLGASVHTCSRNQTEIDQRLQEWQQKG 60

Query: 61  FKVTASVCDLTSKSQRQQLIDTVTSTFNGSLNILVNNAGTVTLKGATEYTSEDYNNMMST 120
           FKVTASVCDLTS SQRQQLIDTV+S FNG+LNILVNNAGTVT+K ATEYT+EDYN MMST
Sbjct: 61  FKVTASVCDLTSSSQRQQLIDTVSSIFNGTLNILVNNAGTVTMKTATEYTTEDYNYMMST 120

Query: 121 NFEAPYHLSQISHSILKASGYGSIVFVSSIAGVTALPKISIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQISH ILKASGYGSIVFVSSIAGVTALP+ISIYAA+KGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISHPILKASGYGSIVFVSSIAGVTALPRISIYAATKGAINQITKNLACEW 180

Query: 181 AKDNIRVNTVAPWGVKTTISKPDPVAMEEYGRLIRRTPAGRIGEPEEISSVVAFLCLPAA 240
           AKDNIR+NTVAPWGV+TTIS PD  A+EEYGRLI RTPAGR+GEPEEISSVVAFLCLPAA
Sbjct: 181 AKDNIRINTVAPWGVRTTISTPDAAAVEEYGRLIGRTPAGRLGEPEEISSVVAFLCLPAA 240

Query: 241 SYVSGQIICVDGGYTAGGW 260
           SYVSGQIICVDGGYTAGGW
Sbjct: 241 SYVSGQIICVDGGYTAGGW 259

BLAST of Clc10G20950 vs. ExPASy TrEMBL
Match: A0A6J1F1Z8 (tropinone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111441431 PE=4 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 9.7e-120
Identity = 218/258 (84.50%), Postives = 238/258 (92.25%), Query Frame = 0

Query: 1   MSSPLQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELHQRLKEWEQKG 60
           M+SP+QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTEL QR+KEWE+KG
Sbjct: 1   MNSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60

Query: 61  FKVTASVCDLTSKSQRQQLIDTVTSTFNGSLNILVNNAGTVTLKGATEYTSEDYNNMMST 120
           F+V+ SVCDL+S+SQRQQLI TV+S FNG LNILVNNAG VTLK A E+T+EDYNN+MST
Sbjct: 61  FEVSGSVCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMST 120

Query: 121 NFEAPYHLSQISHSILKASGYGSIVFVSSIAGVTALPKISIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQIS+  LKASGYGSIVFVSS+AGV ALPKISIYAASKGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGAINQITKNLACEW 180

Query: 181 AKDNIRVNTVAPWGVKTTISKPDPVAMEEYGRLIRRTPAGRIGEPEEISSVVAFLCLPAA 240
           AKDNIR+NTVAPW VKTTIS+PD   +EEY RL+  TPAGRIGE  EISSVVAFLCLPAA
Sbjct: 181 AKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLELTPAGRIGEANEISSVVAFLCLPAA 240

Query: 241 SYVSGQIICVDGGYTAGG 259
           SYV+GQ+ICVDGGYTAGG
Sbjct: 241 SYVTGQVICVDGGYTAGG 258

BLAST of Clc10G20950 vs. ExPASy TrEMBL
Match: A0A6J1J921 (tropinone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111482392 PE=4 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 1.3e-119
Identity = 216/258 (83.72%), Postives = 238/258 (92.25%), Query Frame = 0

Query: 1   MSSPLQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELHQRLKEWEQKG 60
           MSSP+QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSR QTEL QR+KEWE+KG
Sbjct: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKG 60

Query: 61  FKVTASVCDLTSKSQRQQLIDTVTSTFNGSLNILVNNAGTVTLKGATEYTSEDYNNMMST 120
           F+V+ SVCDL+S+S R+QLI TV+S FNG LNILVNNAGT+TLK A E+T+EDYNN+MST
Sbjct: 61  FEVSGSVCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMST 120

Query: 121 NFEAPYHLSQISHSILKASGYGSIVFVSSIAGVTALPKISIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQIS+  LKASGYGSIVFVSS+AGV ALPK+SIYAASKGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180

Query: 181 AKDNIRVNTVAPWGVKTTISKPDPVAMEEYGRLIRRTPAGRIGEPEEISSVVAFLCLPAA 240
           AKDNIR+NTVAPW VKTTISKPD   +EEY RL+ RTPAGRIGE  EISSVVAFLCLPAA
Sbjct: 181 AKDNIRINTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240

Query: 241 SYVSGQIICVDGGYTAGG 259
           SY++GQ+ICVDGGYTAGG
Sbjct: 241 SYMTGQVICVDGGYTAGG 258

BLAST of Clc10G20950 vs. TAIR 10
Match: AT5G06060.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 305.1 bits (780), Expect = 5.5e-83
Identity = 150/254 (59.06%), Postives = 192/254 (75.59%), Query Frame = 0

Query: 6   QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELHQRLKEWEQKGFKVTA 65
           +RWSL G TALVTGGTRGIG A+VEELA  GA VHTCSRNQ EL+  L +W+  G  V+ 
Sbjct: 5   KRWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVSG 64

Query: 66  SVCDLTSKSQRQQLIDTVTSTFNGSLNILVNNAGTVTLKGATEYTSEDYNNMMSTNFEAP 125
           SVCD + + QR++LI   +S F+G LNIL+NN GT   K   EY+SE+Y  +MSTN E+ 
Sbjct: 65  SVCDASVRDQREKLIQEASSAFSGKLNILINNVGTNVRKPTVEYSSEEYAKIMSTNLESA 124

Query: 126 YHLSQISHSILKASGYGSIVFVSSIAGVTALPKISIYAASKGAINQITKNLACEWAKDNI 185
           +HLSQI+H +LKASG GSIVF+SS+AG+  L   SIY A+KGA+NQ+T+NLACEWA DNI
Sbjct: 125 FHLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASDNI 184

Query: 186 RVNTVAPWGVKTTISKPDPVAMEEYGRLIRRTPAGRIGEPEEISSVVAFLCLPAASYVSG 245
           R N VAPW +KT++ +      E    ++ RTP GR+GEPEE+SS+VAFLCLPA+SY++G
Sbjct: 185 RTNCVAPWYIKTSLVETLLEKKEFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYITG 244

Query: 246 QIICVDGGYTAGGW 260
           Q+I VDGG+T  G+
Sbjct: 245 QVISVDGGFTVNGF 258

BLAST of Clc10G20950 vs. TAIR 10
Match: AT2G29290.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 303.1 bits (775), Expect = 2.1e-82
Identity = 145/255 (56.86%), Postives = 201/255 (78.82%), Query Frame = 0

Query: 6   QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELHQRLKEWEQKGFKVTA 65
           +RWSL G  ALVTGGT+GIG A+VEEL+ LGA VHTC+R++T+L +RL+EW++KGF+VT 
Sbjct: 3   KRWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVTT 62

Query: 66  SVCDLTSKSQRQQLIDTVTSTFNGSLNILVNNAGTVTLKGATEYTSEDYNNMMSTNFEAP 125
           S+CD++ + QR++L++TV+S F G LNILVNN GT+ LK  TEYT+E+++ +M+TN ++ 
Sbjct: 63  SICDVSLREQREKLMETVSSLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLDSA 122

Query: 126 YHLSQISHSILKASGYGSIVFVSSIAGVTALPKISIYAASKGAINQITKNLACEWAKDNI 185
           +H+SQ++H +LKASG GSIV +SSIAGV  +   SIY A+KGA+NQ+ +NLACEWA DNI
Sbjct: 123 FHISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASDNI 182

Query: 186 RVNTVAPWGVKTTISKPDPVAMEEYGR-LIRRTPAGRIGEPEEISSVVAFLCLPAASYVS 245
           R N + PW + T +   D +++EE  +    RTP GR+GE  E+S +VAFLCLPAASY++
Sbjct: 183 RTNAICPWLITTPLIS-DLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCLPAASYIT 242

Query: 246 GQIICVDGGYTAGGW 260
           GQ+ICVDGG T  G+
Sbjct: 243 GQVICVDGGLTVNGF 256

BLAST of Clc10G20950 vs. TAIR 10
Match: AT2G29350.1 (senescence-associated gene 13 )

HSP 1 Score: 301.6 bits (771), Expect = 6.1e-82
Identity = 148/253 (58.50%), Postives = 194/253 (76.68%), Query Frame = 0

Query: 7   RWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELHQRLKEWEQKGFKVTAS 66
           RWSL G TALVTGG++GIG A+VEELA LGA VHTC+R++T+L +RL+EW+ KGF+VT S
Sbjct: 12  RWSLGGMTALVTGGSKGIGEAVVEELAMLGAKVHTCARDETQLQERLREWQAKGFQVTTS 71

Query: 67  VCDLTSKSQRQQLIDTVTSTFNGSLNILVNNAGTVTLKGATEYTSEDYNNMMSTNFEAPY 126
           VCD++S+ QR +L++TV+S + G LNILVNN GT   K  TEYT+ED++ +M+TN E+ +
Sbjct: 72  VCDVSSRDQRVKLMETVSSLYQGKLNILVNNVGTSIFKPTTEYTAEDFSFVMATNLESAF 131

Query: 127 HLSQISHSILKASGYGSIVFVSSIAGVTALPKISIYAASKGAINQITKNLACEWAKDNIR 186
           HLSQ++H +LKASG GSIV +SS AGV  +   SIY A+KGA+NQ+ +NLACEWA DNIR
Sbjct: 132 HLSQLAHPLLKASGSGSIVLISSAAGVVHVNVGSIYGATKGAMNQLARNLACEWASDNIR 191

Query: 187 VNTVAPWGVKTTISKPDPVAMEEYGRLIRRTPAGRIGEPEEISSVVAFLCLPAASYVSGQ 246
            N+V PW + T +S  D    E     +R TP GR+GE  E+S +VAFLCLP+ASY++GQ
Sbjct: 192 TNSVCPWYITTPLSN-DFFDEEFKKEAVRTTPMGRVGEANEVSPLVAFLCLPSASYITGQ 251

Query: 247 IICVDGGYTAGGW 260
            ICVDGG T  G+
Sbjct: 252 TICVDGGATVNGF 263

BLAST of Clc10G20950 vs. TAIR 10
Match: AT2G29150.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 301.2 bits (770), Expect = 7.9e-82
Identity = 149/254 (58.66%), Postives = 202/254 (79.53%), Query Frame = 0

Query: 7   RWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELHQRLKEWEQKGFKVTAS 66
           RWSL G TALVTGG++G+G A+VEELA LGA VHTC+R++T+L +RL+EW+ KGF+VT S
Sbjct: 13  RWSLEGMTALVTGGSKGLGEAVVEELAMLGARVHTCARDETQLQERLREWQAKGFEVTTS 72

Query: 67  VCDLTSKSQRQQLIDTVTSTFNGSLNILVNNAGTVTLKGATEYTSEDYNNMMSTNFEAPY 126
           VCD++S+ QR++L++TV+S F G LNILVNNAGT  +K +TEYT+EDY+ +M+TN E+ +
Sbjct: 73  VCDVSSREQREKLMETVSSVFQGKLNILVNNAGTGIIKPSTEYTAEDYSFLMATNLESAF 132

Query: 127 HLSQISHSILKASGYGSIVFVSSIAGVTALPKISIYAASKGAINQITKNLACEWAKDNIR 186
           HLSQI+H +LKASG GSIVF+SS+AG+      SIY ASKGA+NQ+ ++LACEWA DNIR
Sbjct: 133 HLSQIAHPLLKASGSGSIVFMSSVAGLVH-TGASIYGASKGAMNQLGRSLACEWASDNIR 192

Query: 187 VNTVAPWGVKTTISKPDPVAMEEYGRLIR-RTPAGRIGEPEEISSVVAFLCLPAASYVSG 246
           VN+V PW + T ++     + E+  + +  +TP GR+GE  E+SS+VAFLC PAASY++G
Sbjct: 193 VNSVCPWVITTPLTS-FIFSDEKLRKAVEDKTPMGRVGEANEVSSLVAFLCFPAASYITG 252

Query: 247 QIICVDGGYTAGGW 260
           Q ICVDGG +  G+
Sbjct: 253 QTICVDGGASVNGF 264

BLAST of Clc10G20950 vs. TAIR 10
Match: AT2G29340.2 (NAD-dependent epimerase/dehydratase family protein )

HSP 1 Score: 295.4 bits (755), Expect = 4.4e-80
Identity = 146/254 (57.48%), Postives = 188/254 (74.02%), Query Frame = 0

Query: 6   QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELHQRLKEWEQKGFKVTA 65
           +RWSL G TALVTGG  GIGYAIVEELA  GA +H C  ++ +L+Q L EWE+KGF+V+ 
Sbjct: 3   KRWSLKGMTALVTGGASGIGYAIVEELAGFGARIHVCDISEAKLNQSLSEWEKKGFQVSG 62

Query: 66  SVCDLTSKSQRQQLIDTVTSTFNGSLNILVNNAGTVTLKGATEYTSEDYNNMMSTNFEAP 125
           SVCD+ S+ +R++L+ TV+S F+G LNILV+N G +  K  TEYT +D+   +S+N EA 
Sbjct: 63  SVCDVASRPEREELMQTVSSQFDGKLNILVSNVGVIRSKPTTEYTEDDFAFHISSNVEAA 122

Query: 126 YHLSQISHSILKASGYGSIVFVSSIAGVTALPKISIYAASKGAINQITKNLACEWAKDNI 185
           YH SQ+SH +LKASGYGSI+FVSSIAGV +    SIY  +KGA+ Q+ KNLACEWAKD I
Sbjct: 123 YHFSQLSHPLLKASGYGSIIFVSSIAGVISFDAGSIYGLTKGALIQLAKNLACEWAKDGI 182

Query: 186 RVNTVAPWGVKTTISKPDPVAMEEYGRLIRRTPAGRIGEPEEISSVVAFLCLPAASYVSG 245
           R N VAP  + T +S+     +     L+ RTP GR+GEP E++S+VAFLCLPAASY++G
Sbjct: 183 RANAVAPNVINTPLSQSYLEDVSFKKALLSRTPLGRVGEPNEVASLVAFLCLPAASYITG 242

Query: 246 QIICVDGGYTAGGW 260
           Q ICVDGG T  G+
Sbjct: 243 QTICVDGGLTVNGF 256

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008442514.15.6e-13091.89PREDICTED: tropinone reductase homolog [Cucumis melo] >KAA0044113.1 tropinone re... [more]
XP_004137775.16.2e-12990.73tropinone reductase homolog [Cucumis sativus] >KGN58843.1 hypothetical protein C... [more]
XP_038905102.15.3e-12891.51tropinone reductase homolog [Benincasa hispida][more]
XP_023527559.11.4e-12085.27tropinone reductase homolog [Cucurbita pepo subsp. pepo][more]
KAG7017273.13.1e-12084.88hypothetical protein SDJN02_19136, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
P501658.8e-8663.04Tropinone reductase homolog OS=Datura stramonium OX=4076 PE=2 SV=1[more]
P501629.2e-8360.55Tropinone reductase 1 OS=Datura stramonium OX=4076 GN=TR1 PE=1 SV=1[more]
Q9LHT07.7e-8259.06Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g060... [more]
Q9ZW132.9e-8156.86Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g292... [more]
Q9ZW188.6e-8158.50Senescence-associated protein 13 OS=Arabidopsis thaliana OX=3702 GN=SAG13 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A5D3DN352.7e-13091.89Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3B6I22.7e-13091.89tropinone reductase homolog OS=Cucumis melo OX=3656 GN=LOC103486364 PE=4 SV=1[more]
A0A0A0LAG73.0e-12990.73Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G733970 PE=4 SV=1[more]
A0A6J1F1Z89.7e-12084.50tropinone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111441431 PE=4 S... [more]
A0A6J1J9211.3e-11983.72tropinone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111482392 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
AT5G06060.15.5e-8359.06NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29290.22.1e-8256.86NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29350.16.1e-8258.50senescence-associated gene 13 [more]
AT2G29150.17.9e-8258.66NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29340.24.4e-8057.48NAD-dependent epimerase/dehydratase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 89..100
score: 50.55
coord: 162..181
score: 38.33
coord: 142..150
score: 44.0
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 218..238
score: 37.39
coord: 14..31
score: 50.59
coord: 183..200
score: 38.8
coord: 89..100
score: 56.22
coord: 162..181
score: 41.85
coord: 136..152
score: 34.58
NoneNo IPR availableGENE3D3.40.50.720coord: 2..258
e-value: 1.7E-81
score: 275.2
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 21..255
e-value: 1.8E-57
score: 194.6
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 16..199
e-value: 1.3E-18
score: 65.1
NoneNo IPR availablePIRSRPIRSR000094-3PIRSR000094-3coord: 10..256
e-value: 8.8E-19
score: 65.5
NoneNo IPR availablePANTHERPTHR42898:SF28TROPINONE REDUCTASE-LIKE PROTEINcoord: 5..258
IPR045000Tropinone reductasePANTHERPTHR42898TROPINONE REDUCTASEcoord: 5..258
IPR020904Short-chain dehydrogenase/reductase, conserved sitePROSITEPS00061ADH_SHORTcoord: 149..177
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 14..257

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc10G20950.1Clc10G20950.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity