Clc10G14500 (gene) Watermelon (cordophanus) v2

Overview


Name	Clc10G14500
Type	gene
Organism	Citrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
Description	Ribonuclease T(2)
Location	ClcChr10: 28169716 .. 28170542 (+)
RNA-Seq Expression	Clc10G14500
Synteny	Clc10G14500

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAACGTTAAATAGAGATATTATGTTCTTTGTGTCATTTGTGTTAATCTTTTTGTTTTCTATTGTTAAATCACAAACTTTCGATAATTTTTGGTTTGTTCAACAATGGCCACCAGCTGTTTGTGCTTTACAATCTGGACGATGTGTAGGACGAGGGACTCGATCCTTTACAATCCATGGTTTGTGGCCTCAAAAGGGAGGAAACTCTGTAACTAATTGCGCTGGCACTCCATTTGATTTCACTAAGGTAAAATTCATACACCCAAAGTCTCTAATTTTCTTGATTATTAATCATGCATCGATTCTAACTAAGAACCATTTTAAAATTAAGAAAAAGTTTATGATGCTAAAACATATTCATTTATAAGCTTAAAAGAAACACAAATATACTCAACATATAAGAATGTGTGGGCATTTCAGATTGCGCATTTAGAAAGCGATCTGAACATAGTTTGGACAAATGTGGTAACGGGAAACAACAAATTCTTTTGGGGTTACGAGTGGAATAAACATGGAATTTGCTCAGAAAGCAAGTTTGACATGGCGAAGTACTTCCAAACGGCGATAAACATGAGGCACAATATAGACCTTCTTAGCGTAATGAGAACTGGTGGAATTGTACCAAACGGAGCCTCAAAGGCGAAGCAAAGAGTAGAAAGTGCAATCTCATCCCATTTTGGTAAGGACCCAATTCTTCGGTGTAAAACAGCACCAAACGGCCAAGTTTTGTTGACAGAGATTGTTATGTGCTTTGATGACGATGGTGTCACCCTTCTACACTGCAACAAAGCAAGATCCAATTGTGCTGGGAGCTTTATTTTTTGA

mRNA sequence

ATGAAAACGTTAAATAGAGATATTATGTTCTTTGTGTCATTTGTGTTAATCTTTTTGTTTTCTATTGTTAAATCACAAACTTTCGATAATTTTTGGTTTGTTCAACAATGGCCACCAGCTGTTTGTGCTTTACAATCTGGACGATGTGTAGGACGAGGGACTCGATCCTTTACAATCCATGGTTTGTGGCCTCAAAAGGGAGGAAACTCTGTAACTAATTGCGCTGGCACTCCATTTGATTTCACTAAGATTGCGCATTTAGAAAGCGATCTGAACATAGTTTGGACAAATGTGGTAACGGGAAACAACAAATTCTTTTGGGGTTACGAGTGGAATAAACATGGAATTTGCTCAGAAAGCAAGTTTGACATGGCGAAGTACTTCCAAACGGCGATAAACATGAGGCACAATATAGACCTTCTTAGCGTAATGAGAACTGGTGGAATTGTACCAAACGGAGCCTCAAAGGCGAAGCAAAGAGTAGAAAGTGCAATCTCATCCCATTTTGGTAAGGACCCAATTCTTCGGTGTAAAACAGCACCAAACGGCCAAGTTTTGTTGACAGAGATTGTTATGTGCTTTGATGACGATGGTGTCACCCTTCTACACTGCAACAAAGCAAGATCCAATTGTGCTGGGAGCTTTATTTTTTGA

Coding sequence (CDS)

ATGAAAACGTTAAATAGAGATATTATGTTCTTTGTGTCATTTGTGTTAATCTTTTTGTTTTCTATTGTTAAATCACAAACTTTCGATAATTTTTGGTTTGTTCAACAATGGCCACCAGCTGTTTGTGCTTTACAATCTGGACGATGTGTAGGACGAGGGACTCGATCCTTTACAATCCATGGTTTGTGGCCTCAAAAGGGAGGAAACTCTGTAACTAATTGCGCTGGCACTCCATTTGATTTCACTAAGATTGCGCATTTAGAAAGCGATCTGAACATAGTTTGGACAAATGTGGTAACGGGAAACAACAAATTCTTTTGGGGTTACGAGTGGAATAAACATGGAATTTGCTCAGAAAGCAAGTTTGACATGGCGAAGTACTTCCAAACGGCGATAAACATGAGGCACAATATAGACCTTCTTAGCGTAATGAGAACTGGTGGAATTGTACCAAACGGAGCCTCAAAGGCGAAGCAAAGAGTAGAAAGTGCAATCTCATCCCATTTTGGTAAGGACCCAATTCTTCGGTGTAAAACAGCACCAAACGGCCAAGTTTTGTTGACAGAGATTGTTATGTGCTTTGATGACGATGGTGTCACCCTTCTACACTGCAACAAAGCAAGATCCAATTGTGCTGGGAGCTTTATTTTTTGA

Protein sequence

MKTLNRDIMFFVSFVLIFLFSIVKSQTFDNFWFVQQWPPAVCALQSGRCVGRGTRSFTIHGLWPQKGGNSVTNCAGTPFDFTKIAHLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSESKFDMAKYFQTAINMRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTAPNGQVLLTEIVMCFDDDGVTLLHCNKARSNCAGSFIF

Homology

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BLAST of Clc10G14500 vs. NCBI nr
Match: XP_008443402.1 (PREDICTED: ribonuclease MC-like [Cucumis melo])

HSP 1 Score: 404.8 bits (1039), Expect = 4.6e-109
Identity = 188/217 (86.64%), Postives = 201/217 (92.63%), Query Frame = 0


Query: 1   MKTLNRDIMFFVSFVLIFLFSIVKSQTFDNFWFVQQWPPAVCALQSGRCVGRGTRSFTIH 60
           M+   RD++FFV FVL  LF IVKSQTFDNFWFVQQWPPA+C LQSGRCVGRGTRSFTIH
Sbjct: 1   MEKTKRDVVFFV-FVLTILFPIVKSQTFDNFWFVQQWPPAICTLQSGRCVGRGTRSFTIH 60

Query: 61  GLWPQKGGNSVTNCAGTPFDFTKIAHLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSES 120
           GLWPQKGG SVTNC+G  FDFTKIAHLESDLN+VW NVVTGNNKFFWG+EWNKHGICSES
Sbjct: 61  GLWPQKGGRSVTNCSGNSFDFTKIAHLESDLNVVWPNVVTGNNKFFWGHEWNKHGICSES 120

Query: 121 KFDMAKYFQTAINMRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTA 180
           KFD AKYFQTAINMRH+IDLLSV+RTGGIVPNGASKAKQRVE+AISSHF KDPILRCKTA
Sbjct: 121 KFDQAKYFQTAINMRHSIDLLSVLRTGGIVPNGASKAKQRVETAISSHFAKDPILRCKTA 180

Query: 181 PNGQVLLTEIVMCFDDDGVTLLHCNKARSNCAGSFIF 218
            NGQVLLTEIV+CFDDDGVTL++CNKARSNCA SFIF
Sbjct: 181 SNGQVLLTEIVICFDDDGVTLINCNKARSNCARSFIF 216

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BLAST of Clc10G14500 vs. NCBI nr
Match: XP_004147499.1 (ribonuclease MC [Cucumis sativus] >KGN59560.1 hypothetical protein Csa_002553 [Cucumis sativus])

HSP 1 Score: 401.4 bits (1030), Expect = 5.1e-108
Identity = 187/216 (86.57%), Postives = 199/216 (92.13%), Query Frame = 0


Query: 2   KTLNRDIMFFVSFVLIFLFSIVKSQTFDNFWFVQQWPPAVCALQSGRCVGRGTRSFTIHG 61
           KT + D++FFV FVL  LF IVKSQTFD+FWFVQQWPPAVC LQSGRCVGRGTRSFTIHG
Sbjct: 3   KTKSVDVVFFV-FVLTILFPIVKSQTFDDFWFVQQWPPAVCTLQSGRCVGRGTRSFTIHG 62

Query: 62  LWPQKGGNSVTNCAGTPFDFTKIAHLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSESK 121
           LWPQKGG SVTNC G  FDFTKIAHLE+DLN+VW NVVTGNNKFFWG+EWNKHGICSESK
Sbjct: 63  LWPQKGGRSVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSESK 122

Query: 122 FDMAKYFQTAINMRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTAP 181
           FD AKYFQTAINMRH IDLLSV+RTGG+ PNGASKAKQRVE+AISSHFGKDPILRCK A 
Sbjct: 123 FDEAKYFQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKAS 182

Query: 182 NGQVLLTEIVMCFDDDGVTLLHCNKARSNCAGSFIF 218
           NGQVLLTEIVMCFDDDGVTL++CNKARSNCAGSFIF
Sbjct: 183 NGQVLLTEIVMCFDDDGVTLINCNKARSNCAGSFIF 217

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BLAST of Clc10G14500 vs. NCBI nr
Match: XP_038905534.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 398.3 bits (1022), Expect = 4.3e-107
Identity = 186/217 (85.71%), Postives = 199/217 (91.71%), Query Frame = 0


Query: 1   MKTLNRDIMFFVSFVLIFLFSIVKSQTFDNFWFVQQWPPAVCALQSGRCVGRGTRSFTIH 60
           M T  R+I+  V F L  LF IV+SQTFD+FWFVQQWPPAVCALQSGRCVGRGTRSFTIH
Sbjct: 1   MATTKRNIVLLV-FGLTILFPIVQSQTFDDFWFVQQWPPAVCALQSGRCVGRGTRSFTIH 60

Query: 61  GLWPQKGGNSVTNCAGTPFDFTKIAHLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSES 120
           GLWPQKGG+SVTNCAG  FDFTKIAHLE+DLNIVW NV+TGNNKFFWG+EWNKHGICSES
Sbjct: 61  GLWPQKGGSSVTNCAGNSFDFTKIAHLENDLNIVWPNVITGNNKFFWGHEWNKHGICSES 120

Query: 121 KFDMAKYFQTAINMRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTA 180
           KFD A YFQTAINMRH+IDLLSVMRTGG++PNGASKAKQRVES ISSHFGKDPILRCKTA
Sbjct: 121 KFDQAAYFQTAINMRHSIDLLSVMRTGGVIPNGASKAKQRVESVISSHFGKDPILRCKTA 180

Query: 181 PNGQVLLTEIVMCFDDDGVTLLHCNKARSNCAGSFIF 218
            NGQVLLTE+VMCFDDDGVTLL+CNKARSNCA SFIF
Sbjct: 181 SNGQVLLTEVVMCFDDDGVTLLNCNKARSNCATSFIF 216

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BLAST of Clc10G14500 vs. NCBI nr
Match: XP_022935695.1 (ribonuclease MC-like [Cucurbita moschata])

HSP 1 Score: 352.1 bits (902), Expect = 3.5e-93
Identity = 161/217 (74.19%), Postives = 184/217 (84.79%), Query Frame = 0


Query: 1   MKTLNRDIMFFVSFVLIFLFSIVKSQTFDNFWFVQQWPPAVCALQSGRCVGRGTRSFTIH 60
           M T N  I  F+ FV + L  + KSQ FD+FWFVQQWPP VCALQSGRCVGRGT SFTIH
Sbjct: 1   MATPNTQI--FLLFVFVSLLPMAKSQPFDDFWFVQQWPPNVCALQSGRCVGRGTNSFTIH 60

Query: 61  GLWPQKGGNSVTNCAGTPFDFTKIAHLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSES 120
           GLWPQKGGNSVTNC GT FDF +I+HLE+DLNIVW N++TGN+K+FWG+EWN HGICSES
Sbjct: 61  GLWPQKGGNSVTNCPGTSFDFNQISHLENDLNIVWPNLITGNHKWFWGHEWNTHGICSES 120

Query: 121 KFDMAKYFQTAINMRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTA 180
           KFD   YFQT+IN+RHNI+LLS +R  G+VPNGASK+KQRVESAI SHF K P+LRCKTA
Sbjct: 121 KFDQKAYFQTSINLRHNINLLSALRVQGVVPNGASKSKQRVESAIISHFKKIPVLRCKTA 180

Query: 181 PNGQVLLTEIVMCFDDDGVTLLHCNKARSNCAGSFIF 218
            NGQVLLTEIVMC DDDGVTL++CN A+SNCA +FIF
Sbjct: 181 SNGQVLLTEIVMCIDDDGVTLINCNIAKSNCANNFIF 215

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BLAST of Clc10G14500 vs. NCBI nr
Match: XP_023528964.1 (ribonuclease MC-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 350.5 bits (898), Expect = 1.0e-92
Identity = 160/217 (73.73%), Postives = 183/217 (84.33%), Query Frame = 0


Query: 1   MKTLNRDIMFFVSFVLIFLFSIVKSQTFDNFWFVQQWPPAVCALQSGRCVGRGTRSFTIH 60
           M T N  I  F+ FV   L    KSQ FD+FWFVQQWPP VCALQSGRC+GRGT SFTIH
Sbjct: 1   MATPNTQI--FLLFVFAILLPRTKSQPFDDFWFVQQWPPNVCALQSGRCIGRGTNSFTIH 60

Query: 61  GLWPQKGGNSVTNCAGTPFDFTKIAHLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSES 120
           GLWPQKGGNSVTNC GT FDF +I+HLE+DLNIVW N++TGN+K+FWG+EWN HGICSES
Sbjct: 61  GLWPQKGGNSVTNCPGTSFDFNQISHLENDLNIVWPNLITGNHKWFWGHEWNTHGICSES 120

Query: 121 KFDMAKYFQTAINMRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTA 180
           K+D   YFQT+IN+RHNI+LLS +R  G+VPNGASK+KQRVESAI SHF K+P+LRCKTA
Sbjct: 121 KYDEKAYFQTSINLRHNINLLSALRVQGVVPNGASKSKQRVESAIISHFKKNPVLRCKTA 180

Query: 181 PNGQVLLTEIVMCFDDDGVTLLHCNKARSNCAGSFIF 218
            NGQVLLTEIVMC DDDGVTL++CN A+SNCA SFIF
Sbjct: 181 SNGQVLLTEIVMCIDDDGVTLINCNLAKSNCANSFIF 215

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BLAST of Clc10G14500 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.0e-58
Identity = 102/193 (52.85%), Postives = 134/193 (69.43%), Query Frame = 0


Query: 28  FDNFWFVQQWPPAVCALQ-SGRCVGRGTRSFTIHGLWPQKGGNSVTNC-AGTPFDFTKIA 87
           FD+FWFVQQWPPAVC+ Q SG C G G R+FTIHGLWPQ  G S+TNC  G+PFD TKI+
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKIS 60

Query: 88  HLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSESKFDMAKYFQTAINMRHNIDLLSVMR 147
           HL+S LN +W NV+  NN+ FW +EW KHG CSES F+ A YF+ A++MR+N D++  +R
Sbjct: 61  HLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALR 120

Query: 148 TGGIVPNGASKAKQRVESAISSHFGKDPILRCKTAPNGQV-LLTEIVMCFDDDGVTLLHC 207
                PNG +K++Q ++  + + FGK P LRC+T P  +V  L ++V CF  DG TL+ C
Sbjct: 121 PHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTLIDC 180

Query: 208 NKARSNCAGSFIF 218
              R  C  +FIF
Sbjct: 181 --TRDTCGANFIF 191

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BLAST of Clc10G14500 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 2.8e-32
Identity = 69/187 (36.90%), Postives = 100/187 (53.48%), Query Frame = 0


Query: 25  SQTFDNFWFVQQWPPAVCALQSGRCV---GRGTRSFTIHGLWPQ-KGGNSVTNC-AGTPF 84
           S+ FD F+FVQQWP + C  Q   C    G+    F IHGLWP  K G   +NC A  PF
Sbjct: 27  SEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWPNYKDGTYPSNCDASKPF 86

Query: 85  DFTKIAHLESDLNIVWTNVV--TGNNKFFWGYEWNKHGICSESKFDMAKYFQTAINMRHN 144
           D + I+ L + +   W  +   +G+ + FW +EW KHG CSES  D  +YFQTA+N++  
Sbjct: 87  DSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQHEYFQTALNLKQK 146

Query: 145 IDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTAPNGQVLLTEIVMCFDDD 204
            +LL  +   GI P+G S + + +  +I    G  P + C    +G   L ++ +C D  
Sbjct: 147 TNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRDGSGNSQLYQVYLCVDRS 206

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BLAST of Clc10G14500 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 127.9 bits (320), Expect = 1.4e-28
Identity = 66/205 (32.20%), Postives = 111/205 (54.15%), Query Frame = 0


Query: 9   MFFVSFVLIFLFSIVK-SQTFDNFWFVQQWPPAVCALQSGRC---VGRGTRSFTIHGLWP 68
           +F +  ++    S++  ++ FD F+FVQQWP + C  +   C    G+    F IHGLWP
Sbjct: 9   LFLILLIITQCLSVLNAAKDFDFFYFVQQWPGSYCDTKQSCCYPTTGKPAADFGIHGLWP 68

Query: 69  QKG-GNSVTNC-AGTPFDFTKIAHLESDLNIVWTNVV--TGNNKFFWGYEWNKHGICSES 128
               G   +NC   +P+D ++I+ L S +   W  +   +G+   FW +EW KHG C+ES
Sbjct: 69  NNNDGTYPSNCDPNSPYDQSQISDLISSMQQNWPTLACPSGSGSTFWSHEWEKHGTCAES 128

Query: 129 KF-DMAKYFQTAINMRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKT 188
              +   YF+ A+++++ IDLLS+++   I P+G S     + +AI S  G  P ++C  
Sbjct: 129 VLTNQHAYFKKALDLKNQIDLLSILQGADIHPDGESYDLVNIRNAIKSAIGYTPWIQCNV 188

Query: 189 APNGQVLLTEIVMCFDDDGVTLLHC 205
             +G   L ++ +C D  G +L+ C
Sbjct: 189 DQSGNSQLYQVYICVDGSGSSLIEC 213

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BLAST of Clc10G14500 vs. ExPASy Swiss-Prot
Match: P80196 (Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2)

HSP 1 Score: 107.8 bits (268), Expect = 1.5e-22
Identity = 64/212 (30.19%), Postives = 97/212 (45.75%), Query Frame = 0


Query: 1   MKTLNRDIMFFVSFVLIFLFSIVKSQTFDNFWFVQQWPPAVCALQSGRC---VGRGTRSF 60
           M    + ++  +  V   L   V SQ FD F+FVQQWP + C  +   C    G+    F
Sbjct: 1   MMKSQKKLLIKIIVVQCLLVLCVTSQDFDFFYFVQQWPASYCDTRRSCCYPTTGKPDEDF 60

Query: 61  TIHGLWPQ-KGGNSVTNC-AGTPFDFTKIAHLESDLNIVWTNVV--TGNNKFFWGYEWNK 120
           +IHGLWP  K G    NC   +  D ++ + L S +   W ++   + +   FW +EW K
Sbjct: 61  SIHGLWPNYKDGKWPQNCDRESSLDESEFSDLISTMEKNWPSLACPSSDGLKFWSHEWLK 120

Query: 121 HGICSESKFDMAKYFQTAINMRHNIDLLSVMRTGGIVP-NGASKAKQRVESAISSHFGKD 180
           HG C  S  +   YFQTA++ +   +LL  +   GI P NG     + ++ AI    G  
Sbjct: 121 HGTC--SALNQHAYFQTALDFKTKSNLLQNLNNAGIKPRNGDYYGVESIKKAIEKGVGHT 180

Query: 181 PILRCKTAPNGQVLLTEIVMCFDDDGVTLLHC 205
           P + C     G   L ++ +C D      + C
Sbjct: 181 PFIECNVDSQGNHQLYQVYLCVDSSASKFIDC 210

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BLAST of Clc10G14500 vs. ExPASy Swiss-Prot
Match: O80322 (Ribonuclease S-1 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 1.9e-20
Identity = 62/202 (30.69%), Postives = 92/202 (45.54%), Query Frame = 0


Query: 9   MFFVSFVLIFLFSIVKSQTFDNFWFVQQWPPAVCALQSGRCVGRGTRSFTIHGLWP-QKG 68
           MF + F LI L     +  +D F F QQ+ PAVC      C     + FT+HGLWP  + 
Sbjct: 9   MFTMVFSLIVLILSSSTVGYDYFQFTQQYQPAVCNSNPTPCNDPTDKLFTVHGLWPSNRN 68

Query: 69  GNSVTNCAGTPFDFTKIAHLESDLNIVWTNVVTGNNKF-FWGYEWNKHGICSESKF-DMA 128
           G     C  T  +  KI ++ + L I+W NV+  ++   FW  EW KHG C      D  
Sbjct: 69  GPDPEKCKTTALNSQKIGNMTAQLEIIWPNVLNRSDHVGFWEKEWIKHGTCGYPTIKDDM 128

Query: 129 KYFQTAINM--RHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGK-DPILRCKTAPN 188
            Y QT I M      ++ +++    I PNG ++    +E+AI        P  +C+    
Sbjct: 129 HYLQTVIRMYITQKQNVSAILSKAAIQPNGTNRPLVDIENAIRRGTNNTKPKFKCQKNTR 188

Query: 189 GQVLLTEIVMCFDDDGVTLLHC 205
               L E+ +C D D    ++C
Sbjct: 189 TTTELVEVTLCSDRDLKKFINC 210

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BLAST of Clc10G14500 vs. ExPASy TrEMBL
Match: A0A1S3B8Q3 (Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103486996 PE=3 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 2.2e-109
Identity = 188/217 (86.64%), Postives = 201/217 (92.63%), Query Frame = 0


Query: 1   MKTLNRDIMFFVSFVLIFLFSIVKSQTFDNFWFVQQWPPAVCALQSGRCVGRGTRSFTIH 60
           M+   RD++FFV FVL  LF IVKSQTFDNFWFVQQWPPA+C LQSGRCVGRGTRSFTIH
Sbjct: 1   MEKTKRDVVFFV-FVLTILFPIVKSQTFDNFWFVQQWPPAICTLQSGRCVGRGTRSFTIH 60

Query: 61  GLWPQKGGNSVTNCAGTPFDFTKIAHLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSES 120
           GLWPQKGG SVTNC+G  FDFTKIAHLESDLN+VW NVVTGNNKFFWG+EWNKHGICSES
Sbjct: 61  GLWPQKGGRSVTNCSGNSFDFTKIAHLESDLNVVWPNVVTGNNKFFWGHEWNKHGICSES 120

Query: 121 KFDMAKYFQTAINMRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTA 180
           KFD AKYFQTAINMRH+IDLLSV+RTGGIVPNGASKAKQRVE+AISSHF KDPILRCKTA
Sbjct: 121 KFDQAKYFQTAINMRHSIDLLSVLRTGGIVPNGASKAKQRVETAISSHFAKDPILRCKTA 180

Query: 181 PNGQVLLTEIVMCFDDDGVTLLHCNKARSNCAGSFIF 218
            NGQVLLTEIV+CFDDDGVTL++CNKARSNCA SFIF
Sbjct: 181 SNGQVLLTEIVICFDDDGVTLINCNKARSNCARSFIF 216

[Close]

BLAST of Clc10G14500 vs. ExPASy TrEMBL
Match: A0A0A0LC37 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 2.5e-108
Identity = 187/216 (86.57%), Postives = 199/216 (92.13%), Query Frame = 0


Query: 2   KTLNRDIMFFVSFVLIFLFSIVKSQTFDNFWFVQQWPPAVCALQSGRCVGRGTRSFTIHG 61
           KT + D++FFV FVL  LF IVKSQTFD+FWFVQQWPPAVC LQSGRCVGRGTRSFTIHG
Sbjct: 3   KTKSVDVVFFV-FVLTILFPIVKSQTFDDFWFVQQWPPAVCTLQSGRCVGRGTRSFTIHG 62

Query: 62  LWPQKGGNSVTNCAGTPFDFTKIAHLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSESK 121
           LWPQKGG SVTNC G  FDFTKIAHLE+DLN+VW NVVTGNNKFFWG+EWNKHGICSESK
Sbjct: 63  LWPQKGGRSVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSESK 122

Query: 122 FDMAKYFQTAINMRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTAP 181
           FD AKYFQTAINMRH IDLLSV+RTGG+ PNGASKAKQRVE+AISSHFGKDPILRCK A 
Sbjct: 123 FDEAKYFQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKAS 182

Query: 182 NGQVLLTEIVMCFDDDGVTLLHCNKARSNCAGSFIF 218
           NGQVLLTEIVMCFDDDGVTL++CNKARSNCAGSFIF
Sbjct: 183 NGQVLLTEIVMCFDDDGVTLINCNKARSNCAGSFIF 217

[Close]

BLAST of Clc10G14500 vs. ExPASy TrEMBL
Match: A0A6J1FBD8 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111442477 PE=3 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 1.7e-93
Identity = 161/217 (74.19%), Postives = 184/217 (84.79%), Query Frame = 0


Query: 1   MKTLNRDIMFFVSFVLIFLFSIVKSQTFDNFWFVQQWPPAVCALQSGRCVGRGTRSFTIH 60
           M T N  I  F+ FV + L  + KSQ FD+FWFVQQWPP VCALQSGRCVGRGT SFTIH
Sbjct: 1   MATPNTQI--FLLFVFVSLLPMAKSQPFDDFWFVQQWPPNVCALQSGRCVGRGTNSFTIH 60

Query: 61  GLWPQKGGNSVTNCAGTPFDFTKIAHLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSES 120
           GLWPQKGGNSVTNC GT FDF +I+HLE+DLNIVW N++TGN+K+FWG+EWN HGICSES
Sbjct: 61  GLWPQKGGNSVTNCPGTSFDFNQISHLENDLNIVWPNLITGNHKWFWGHEWNTHGICSES 120

Query: 121 KFDMAKYFQTAINMRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTA 180
           KFD   YFQT+IN+RHNI+LLS +R  G+VPNGASK+KQRVESAI SHF K P+LRCKTA
Sbjct: 121 KFDQKAYFQTSINLRHNINLLSALRVQGVVPNGASKSKQRVESAIISHFKKIPVLRCKTA 180

Query: 181 PNGQVLLTEIVMCFDDDGVTLLHCNKARSNCAGSFIF 218
            NGQVLLTEIVMC DDDGVTL++CN A+SNCA +FIF
Sbjct: 181 SNGQVLLTEIVMCIDDDGVTLINCNIAKSNCANNFIF 215

[Close]

BLAST of Clc10G14500 vs. ExPASy TrEMBL
Match: A0A6J1F776 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441482 PE=3 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 1.9e-92
Identity = 155/207 (74.88%), Postives = 178/207 (85.99%), Query Frame = 0


Query: 11  FVSFVLIFLFSIVKSQTFDNFWFVQQWPPAVCALQSGRCVGRGTRSFTIHGLWPQKGGNS 70
           F+ F    L  I KSQ FD+FWFVQQWPP VCALQSGRCVGRGT+SFTIHGLWPQK GNS
Sbjct: 9   FLLFAFAILIPIAKSQPFDDFWFVQQWPPNVCALQSGRCVGRGTKSFTIHGLWPQKRGNS 68

Query: 71  VTNCAGTPFDFTKIAHLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSESKFDMAKYFQT 130
           VTNC GT FDF +IAHLE+DLN+VW N++TGN+K+FWG+EWN HGICSES+FD A YFQT
Sbjct: 69  VTNCPGTSFDFNQIAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQT 128

Query: 131 AINMRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTAPNGQVLLTEI 190
           +IN+RHNIDLLS +R  G+VPNGASK+KQRVE+AI  HF  +P+LRCKTA NGQVLLTEI
Sbjct: 129 SINLRHNIDLLSALRVQGVVPNGASKSKQRVENAIIKHFQNNPVLRCKTASNGQVLLTEI 188

Query: 191 VMCFDDDGVTLLHCNKARSNCAGSFIF 218
           VMC DDDGVTL++CN A+SNCA SFIF
Sbjct: 189 VMCIDDDGVTLINCNLAKSNCANSFIF 215

[Close]

BLAST of Clc10G14500 vs. ExPASy TrEMBL
Match: A0A6J1J1Y7 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111481960 PE=3 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 7.2e-92
Identity = 158/217 (72.81%), Postives = 180/217 (82.95%), Query Frame = 0


Query: 1   MKTLNRDIMFFVSFVLIFLFSIVKSQTFDNFWFVQQWPPAVCALQSGRCVGRGTRSFTIH 60
           M T N  I  F+ F    L  I KSQ FD+FWFVQQWPP VCALQ GRCVGRGT SFTIH
Sbjct: 1   MTTPNTQI--FLLFAFAILIPIAKSQPFDDFWFVQQWPPNVCALQLGRCVGRGTNSFTIH 60

Query: 61  GLWPQKGGNSVTNCAGTPFDFTKIAHLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSES 120
           GLWPQK GNSVTNC GT FDF +IAHLE+DLN+VW N++TGN+K+FWG+EWN HGICSES
Sbjct: 61  GLWPQKRGNSVTNCPGTSFDFNQIAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSES 120

Query: 121 KFDMAKYFQTAINMRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTA 180
           +FD A YFQT+IN+RHNIDLLS +R  G+VPNGASK+KQRVE+AI  HF  +P+LRCKTA
Sbjct: 121 RFDEAAYFQTSINLRHNIDLLSALRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTA 180

Query: 181 PNGQVLLTEIVMCFDDDGVTLLHCNKARSNCAGSFIF 218
            NGQVLLTEIVMC DDDGVTL++CN A+SNCA SFIF
Sbjct: 181 SNGQVLLTEIVMCIDDDGVTLINCNLAKSNCANSFIF 215

[Close]

BLAST of Clc10G14500 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 140.2 bits (352), Expect = 2.0e-33
Identity = 69/187 (36.90%), Postives = 100/187 (53.48%), Query Frame = 0


Query: 25  SQTFDNFWFVQQWPPAVCALQSGRCV---GRGTRSFTIHGLWPQ-KGGNSVTNC-AGTPF 84
           S+ FD F+FVQQWP + C  Q   C    G+    F IHGLWP  K G   +NC A  PF
Sbjct: 27  SEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWPNYKDGTYPSNCDASKPF 86

Query: 85  DFTKIAHLESDLNIVWTNVV--TGNNKFFWGYEWNKHGICSESKFDMAKYFQTAINMRHN 144
           D + I+ L + +   W  +   +G+ + FW +EW KHG CSES  D  +YFQTA+N++  
Sbjct: 87  DSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQHEYFQTALNLKQK 146

Query: 145 IDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTAPNGQVLLTEIVMCFDDD 204
            +LL  +   GI P+G S + + +  +I    G  P + C    +G   L ++ +C D  
Sbjct: 147 TNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRDGSGNSQLYQVYLCVDRS 206

[Close]

BLAST of Clc10G14500 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 124.0 bits (310), Expect = 1.5e-28
Identity = 67/199 (33.67%), Postives = 99/199 (49.75%), Query Frame = 0


Query: 15  VLIF--LFSIVKSQTFDNFWFVQQWPPAVCALQSGRCV---GRGTRSFTIHGLWPQ-KGG 74
           +L+F  LF     + FD F+FV QWP A C      C    G+    F IHGLWP   GG
Sbjct: 13  LLVFQGLFVSRPQEDFDFFYFVLQWPGAYCDTSRACCYPTSGKPAADFGIHGLWPNYNGG 72

Query: 75  NSVTNC-AGTPFDFTKIAHLESDLNIVWTNVVTGNNK--FFWGYEWNKHGICSESKFDMA 134
           +  +NC   + FD ++I+ L S L   W  +   +N+   FW +EW KHG CSES  D  
Sbjct: 73  SWPSNCDPDSQFDRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSESVMDQH 132

Query: 135 KYFQTAINMRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTAPNGQV 194
           +YF+ A+ ++   +LL +++  GI P+       ++ +AI    G  P + C   P    
Sbjct: 133 EYFENALKLKQKANLLQILKNSGINPDDGFYNLDKITNAIKDGIGFTPGIECNKDPERNA 192

Query: 195 LLTEIVMCFDDDGVTLLHC 205
            L +I +C D  G   + C
Sbjct: 193 QLHQIYICVDTSGTEFIEC 211

[Close]

BLAST of Clc10G14500 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 116.3 bits (290), Expect = 3.0e-26
Identity = 67/208 (32.21%), Postives = 97/208 (46.63%), Query Frame = 0


Query: 9   MFFVSFVLIFLFSIVKSQT---FDNFWFVQQWPPAVCALQSGRC---VGRGTRSFTIHGL 68
           +  VS +++    +  SQT   F+ F++V  WP A+C  Q G C    G     F IHGL
Sbjct: 4   IIIVSLLILQSLVVSSSQTEPDFNFFYWVNYWPGAICDSQKGCCPPTKGNTASDFIIHGL 63

Query: 69  WPQ-KGGNSVTNCAGTP-FDFTKIAHLESDLNIVWTN----VVTGNNKFFWGYEWNKHGI 128
           WPQ   G     C  T  FD +KI+ L   +   WT         N    W +EWNKHG 
Sbjct: 64  WPQFNNGTWPAFCDQTNLFDISKISDLVCQMEKKWTEWGVWACPSNETNLWEHEWNKHGT 123

Query: 129 CSESKFDMAKYFQTAINMRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILR 188
           C +S FD   YF+T +  +H + LL+++   GI PN    +   +++AI    G  P + 
Sbjct: 124 CVQSIFDQHSYFRTNLKFKHKVHLLNILIQKGIKPNDGFYSLDEIKNAIKCAIGFAPGIE 183

Query: 189 CKTAPNGQVLLTEIVMCFDDDGVTLLHC 205
           C     G   L +I +C D+     + C
Sbjct: 184 CNEDVKGNKQLFQIYICLDNYAKEFVEC 211

[Close]

BLAST of Clc10G14500 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 111.3 bits (277), Expect = 9.8e-25
Identity = 66/205 (32.20%), Postives = 96/205 (46.83%), Query Frame = 0


Query: 9   MFFVSFVLIFLFSIVKS--QTFDNFWFVQQWPPAVCALQSGRC---VGRGTRSFTIHGLW 68
           M F  F+L      V+S  Q FD F+FV QWP A C  +   C    G+    F IHGLW
Sbjct: 1   MKFFIFILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLW 60

Query: 69  PQ-KGGNSVTNC-AGTPFDFTKIAHLESDLNIVWTNVVTGNN--KFFWGYEWNKHGICSE 128
           P  K G    NC   + FD  +++ L SDL   W  +   +N    FW +EW KHG C+E
Sbjct: 61  PNYKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAE 120

Query: 129 SKFDMAKYFQTAINMRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKT 188
           S+ D   YF+  + ++   +LL  +   GI P+      + +E+ I    G  P + C  
Sbjct: 121 SELDQHDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNK 180

Query: 189 APNGQVLLTEIVMCFDDDGVTLLHC 205
             +    L +I +C D      ++C
Sbjct: 181 DSSHNSQLYQIYLCVDTSASKFINC 205

[Close]

BLAST of Clc10G14500 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 77.0 bits (188), Expect = 2.0e-14
Identity = 58/189 (30.69%), Postives = 81/189 (42.86%), Query Frame = 0


Query: 28  FDNFWFVQQWPPAV------CALQSGRCVGR-GTRSFTIHGLWPQKGGNSVTNCA-GTPF 87
           FD F    QWP         C  ++  C G      FTIHGLWP     S  +C   + F
Sbjct: 32  FDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSDF 91

Query: 88  DFTKIAHLESDLNIVWTNV-------VTGNNKFFWGYEWNKHGICSESKF-DMAKYFQTA 147
              +I+ L   L   W ++         G    FWG+EW KHG CS   F D   YF T 
Sbjct: 92  KEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNYFLTT 151

Query: 148 IN--MRHNI-DLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTAPNGQVLLT 198
           +N  ++HN+ D+L   + G +  N        + +AI + F   P + CK     +  + 
Sbjct: 152 LNLYLKHNVTDVL--YQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVCK-----RDAID 211

The following BLAST results are available for this feature:

BLAST of Clc10G14500 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008443402.1	4.6e-109	86.64	PREDICTED: ribonuclease MC-like [Cucumis melo]	[more]
XP_004147499.1	5.1e-108	86.57	ribonuclease MC [Cucumis sativus] >KGN59560.1 hypothetical protein Csa_002553 [C...	[more]
XP_038905534.1	4.3e-107	85.71	ribonuclease MC-like [Benincasa hispida]	[more]
XP_022935695.1	3.5e-93	74.19	ribonuclease MC-like [Cucurbita moschata]	[more]
XP_023528964.1	1.0e-92	73.73	ribonuclease MC-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Clc10G14500 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	1.0e-58	52.85	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P42813	2.8e-32	36.90	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P80022	1.4e-28	32.20	Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2	[more]
P80196	1.5e-22	30.19	Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2	[more]
O80322	1.9e-20	30.69	Ribonuclease S-1 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1	[more]

BLAST of Clc10G14500 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B8Q3	2.2e-109	86.64	Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103486996 PE=3 SV=1	[more]
A0A0A0LC37	2.5e-108	86.57	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1	[more]
A0A6J1FBD8	1.7e-93	74.19	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111442477 PE=3 SV=1	[more]
A0A6J1F776	1.9e-92	74.88	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441482 PE=3 SV=1	[more]
A0A6J1J1Y7	7.2e-92	72.81	Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111481960 PE=3 SV=1	[more]

BLAST of Clc10G14500 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02990.1	2.0e-33	36.90	ribonuclease 1	[more]
AT1G14220.1	1.5e-28	33.67	Ribonuclease T2 family protein	[more]
AT1G14210.1	3.0e-26	32.21	Ribonuclease T2 family protein	[more]
AT1G26820.1	9.8e-25	32.20	ribonuclease 3	[more]
AT2G39780.1	2.0e-14	30.69	ribonuclease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 28..204 e-value: 6.7E-42 score: 143.7
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 17..212
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 28..215 e-value: 8.6E-54 score: 184.4
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 28..217
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 17..212
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 106..117
IPR018188	Ribonuclease T2, His active site 1	PROSITE	PS00530	RNASE_T2_1	coord: 57..64
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 28..216 e-value: 4.54925E-68 score: 205.259

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Clc10G14500.2	Clc10G14500.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006401	RNA catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding