Clc10G12560 (gene) Watermelon (cordophanus) v2

Overview
NameClc10G12560
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionTryptophan synthase
LocationClcChr10: 26158030 .. 26163603 (-)
RNA-Seq ExpressionClc10G12560
SyntenyClc10G12560
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTCAATAAAGTACGAAAAATTCGGATTTTAAAGCTTCCATTAAAATGGAAATGTCGTCCTAAAACCAGGATTGATTGCTCTCCCAATGGAGCTTTCAAGTGAGATTCAAATCTCTGTTTCTAATTTTTCATTTGGAAGCTTTTCTTTAGAGATCCTCCAACCTTCTCAAATAATCATAACTGCAAGCAGCAGATTCAAACAATATCATTCCCAACTCTCAGATTCTGGTGGGTTTTGATCCTTTTTTACCTTCCTTCTTTGCATCAATGCTATAATAAGCAAGATCTATTGCTGTATGTCAAACCAAACAAAAACTGAACCAACAAAATTCAACCCATCCAAACCATTAAGCCGCCATTTTTTAGCCTCTTGTTCTACAAAACTTCTCCATACAACAACCCCAATACGGCTTGTTTTTTGTAACTGCGGCCGTTGTAAAAATCGACCTCCTAAACTTCGTATTTATTATGTATTTGAATGTGAAGAGTTTTTGAATCGAATTGAATTGGGGGAGAAGAAACCCATTTCACCAATCTAAATTTCCACCAAAACCCTCTTTACCAAATGGACATTGCATTCAAATCTTCGAGGTTTCTTCCGCTCAACAAGATACCCAGTACGCCTGCCTTTTCTTCACGTCCTTGTAAGATTTCAGTCTCCCAATCCAAGCGGTTTGCTCCAATGGCTGCTCTTGCAGCTTCCCCTGCTGTTGGACTGTCCGAAACCTTCAACAAATTGAGAGAACAAGGGAAGGTAAATGTCTCTTTCTACTGTTTACAATGTATCAATAACTTCATCTTTTTCTTTTTTTTTTTTACTCTGCCTTTTCTGTATGAAATCTTTATATGGTTTGTTTGTTTGTTTGAATTTTCAATTGTTTTCATTGGATTATACTTTTTTTGCATTTGGTGTTTTGTTGGTTGGTATTGAAAGGACAAGTTATGTGCTTGTATGTATGGGAGTGATTTTGAAATTGTTGAAATCAACTTTGCATGTTCAAAATAACTTCGAAATATGTCTTTAATCACTGAAAATTAGTTTAATATTTAATTTCAATTTTCATGTAATCAAAATTGGTTTTGAATAATTAAAAACATATTTGAGAGTAATTTTGAAAATGACAAAAGTAATTTTTTGGTATTTGAGAGATTGTCTTTTTGATATCTACTTTAAAAAGTTGTTAAGAACTTATAGGCTTGTGTTTATTTATTCTCTGGACTTTAAAAAGATTATAGTACATTCTTAAACTTGTAAGTAGTTGTTGTTAGCTCCGTCAATTTAAGGCTTGGTTCACCTAATGTTTGAACCGACTTGAAATCAGCTTGCCATCTAATCCGCAAGTAATGCAATCAACATGTCACATAAGCGTTGAATTGACAGAATTGACGATGGAGACCTATTAGACATAAAAGTAAAAGTTTAAGATCTACCAGAAACATTTTGAAGTAATTAGATAGACACAACCTTGAATGGTGAAGGTCCTATTAGAATCTATTTGAAATACTGAGACTAGACTCAACTTCAAATTTATTGGTTCTGTCTGCTACTTTCTATGTGGTCTGATGGGATTTTTTCATTGATCAACGCTGTCCTAACTGCATTCTTGCTTTAAAGGTCGCATTGATCCCCTACATCACTGCTGGTGATCCTGATCTTTCCACCACGGCTGAGGCATTGAAGGTGCTTTCGACTTGTGGATCAGATATAATTGAACTTGGTGTTCCCTATTCGGATCCCTTGGCAGATGGTCCTGTTATACAGGTTACTTTCTTCTCTTTTTTTTTTCCCCTCCCTTTCAATTCTCCATTTCTAATCTATTGTTCCTTATTCGCTGTTTTATGACTGTTTTTCTGTAGGCTGCAGCCACTCGGTCCTTGGCTAGGGAGACAAACTTTAATGCAATCATTTCTATGCTGAAGGGGGTAATATTGTTCTACCATGCAACATGCTGTAAATAATATCAGCTTTTATTCCACTTTGAGGCAATTAGAAAGAATAGCCAAAACTTATTTTGTTCGCTATTTCTATTATGACTTATGATATGACAGATCTTTTTTTTTTCTTTTTTCTTTTTTGGGAAACTAGATATAATGAAGTGTAGTAACGAAATCATTTAGATGATGATGCATTTCGACAGACTGTCAAGTTTAGTACTCAAATTGTTAGGAAACTTGGAGTTTGCATGTTCATTTTTTCACATGGCATCTTCAAATAAAATGTCTATGGAAATATTTACCCAGTACGCTGTGGTTCACTGCCCTTTAGCCTTTCATTCATGAAACATACAGTCTAACATGTTTTTGTTTTGTTTCTGGCTGGTCAGGTGATTCCTGAACTATCTTGTCCAATTTCCCTTTTTACATATTATAACCCAATTCTTAAACGTGGCATTGAGAACTTTATGATGATAATAAAAGATGCTGGTGTGCGAGGTAATTAATGAGCTTGCCAAATTTTCTTCTAATGAATTAGATTTTTTTATAATTAATTTAACACTGTCCTACTTCGAAGATGACATGGTAAAGGCTAGGCCAATGGTTAATTTTGTAAGTATAGGGTTAGGTTACTAGTTAGGTGGTGGTGGCTTGAGCAAGAGGCTACTACCCAATAGGGCAAAATGAATTTACCTTGGAACATTATTTGTGTCTATTTCAATTCCCCGTTGATTATTGAACTTTAGACAATCAACTACATGATTATTTTTCAGGAGCCTAAAATACAACAGTTTAAAAGAATATGCCATTACTCCTAATAGTATTGGATGGGTGCTGATGTTTGAATTCTTTAAAAAACTGGAATATTTTATTTAAAGCTGTATAGAATATTGCATGAGTTTTTCAATATGTTATGAAGTGTTGTCAACCAACCATAACCAACCATAATTCAATGGATAAAGACACCAATTATCATCTCAAATGTCGATGGTTCAATTCCCATGTATTATTACATGTAATGTACAAAATCTTGACTTGGTTTTTCTTTGTTAGAAAGTTCCATGGGAAGTGTGTCATATTTCTCAACATTGGGTTCGTGAATATGTTTGTGGACTGTGCTCACATTTCACTTTCTTACAGGGCTTGTTGTTCCAGATGTTCCTCTTGAGGAGACTGAAATTTTAAGAAAGGAAGCTGTAAAGTACAATATTGAACTGGTAATTTCTTTTTCTCGTAATGGTTGTATAGACTATCTAAATCATTTGCTAGTTTTTAGGTTTTGATATTTATTTCACTTTTACCTTGGGATATTGGACTCTGTGCTTTTAGACCTCTGGAACATGGATGCTGAGCATGTGAAACACTTCACAGATCGTGATAACTGCTTCATTGCAGTTCTTAAAGATTAGTTTTATTAATTGCCTGAACCTGAAGGAAAGACCTTCAGGAGCGGATAATGAGAAGAAACTTTCTGGATCAATTGTTCAACTATACCAACTTGCATTTTTTTCTCTTTATGACCAACTTAATGAGGAAATGAATAGCCAATTCGTTGACTCATTCTTGTTACAGTCACTTTACTATAAGAAAAAAACTTGGTCGTTAAACCTTGAGTTGATTGCTTGTTCTTATGTTTATGTATTTTATATTATTCTTTTACATTGCTAAAGGTACTCTTAACTACACCAACCACGCCAAAAGATAGGATGAAAAACATCGCTGAAGCTTCAGAGGGATTTGTTTATCTTGTAAGGCCTTTTCTGTTTTATCTCTCTTTATATGCTCTAACTATTGATCTTTGTCATCTCATTGGAGCCTACTGGTCTCATTTATAAAAAGATGAAGTTTAGTAATTTTCTTTCTATCTTGAATTTAATATTCAAAATTAATTTAATTCCACACATATTTCTATCACAAAATAAGAAAAGAGGAGTTCATATTGAAGTTCTTTCTTGAAATTATTTTGCTAGCATCATAGAAGCATATCTAGTGTGTTCCCAAGATTCTTTTTTCAGAATGACATTAACAATGTGGGTGTCGATGAGACTTATCCTTTGCCTTATTCCTTCAAATTGACATGCTGGAATGTTGAGAAAGTTTAGACACCTAAGTCTCAGCTCTTATTATACAAGATCATTACCAAAGTTATAGGTAAAAGACTTGAAAGTTCCTTCCAAACTCGATTATTGAATTTCAAAAACCTTTCATTGGGGATTAAAAAGGGGTGAGGTCATTAAGTTGGGCCTCGAAGAAACTGTTGATAAAACTGATTGGAGCTTTTTAAATGCACTTGCTTTGCTGCTTGTCTCTCTGGTGCCAATTCCTCTGTCATTACCCATGGAAAACCAAGAGGGAAGATCAAGGCTTTTCGAGGAATTAAGCCAGGGGACCTCTTCTCCCCTTTTCCTTTGCTCCAAAAGTTAAATGATTCTGAATTAGTGAAAGGATTCAAAGTTGGTAGAAGCAACTTAATTATTTTATCTTATTATACATCTTTTTTATGAGAAACTAAACTTTTGTAGAGATGAATAAAAGGACGAACAATTATGAAAAGAAAATTGCTACTATAAGAGAGACAATGAAATTGAAGAGACCAAAAACGAAAGAAAGTTGCAGTAAAAGGCTCTAATTGTTTCCAAAAGTCTTGATGTTGGATTAACAATATTCCTTTGGGAGAAGAGAGACTGACTTCTGCTTGATGGGAGTATATTGTTCTCGTGGCTTTGTCTTCATGTACAGACAGAATTTTATAGATAGCATAGAGAGAGAGAGAGCAAGGATTTTGATGAGAAAACAATTTTCTCCCTTTATCATTATTGAATTAACGTTTCGTTGTCAAATTAGACTTCTATTGTCATTCACTGTCTTACTTGCGATATGAAATATATTGTTCAAATCGTAGGTGAGCTCCGTTGGAGTTACCGGTACCCGCACATCAGTTAGTGGTAGAGTTCAAACTCTCCTTGAGGAAGTTAAAGAGGTATGTCAATAGTCAATATATGAGGAAGTACTTGCAGTAAATACAAATTTGTACTTGAAAACAAAGTTTTGGACTTCGAAACCTAATGGTGTTTGAAAGGCATTGATTTGCAACTGATGAATAACTTAGACGCCTCACAATCTGCTTTCCACGACAGGTTACGAAAAAACCAGTAGCAGTTGGTTTTGGTATATCAAAACCCGAGCATGTGAAACAGGTAAAGAACTTCCTCACATAACATAACATCCAATCAATTGTACAATCTATTTGATATGTACTTGCTGGTGTTCATAGGTGGCTGACTGGGGGGCAGATGGTATTATTATTGGTAGTGCTATGGTGAAGCTGTTGGGTGAAGCCCAATCCCCTGAGGAAGGATTGAAGGAGCTTGAAAACTTCACCATATCCTTGAAATCTGCACTCTCTTGATGGCATAACCCAGGCTGTTTCTTTCATCTGTAAACAAGTGCTCAACTTGCACTGTCATCCTAAAATTACACTTCCAATATTTCCCTGTTCTTCTTTTTTGAAACAGTATTTCCCTTTTCTTTTAACAGTGAATAAAATTATCAGCTCGTATTTTCTTCGAAGTTATTTGCCATGGAATATTATGAGAATATTTTCTAGTATTTTCTTGGTAGCCGATGGATGATTGCAAG

mRNA sequence

GTTCAATAAAGTACGAAAAATTCGGATTTTAAAGCTTCCATTAAAATGGAAATGTCGTCCTAAAACCAGGATTGATTGCTCTCCCAATGGAGCTTTCAAGTGAGATTCAAATCTCTGTTTCTAATTTTTCATTTGGAAGCTTTTCTTTAGAGATCCTCCAACCTTCTCAAATAATCATAACTGCAAGCAGCAGATTCAAACAATATCATTCCCAACTCTCAGATTCTGGTGGGTTTTGATCCTTTTTTACCTTCCTTCTTTGCATCAATGCTATAATAAGCAAGATCTATTGCTGTATGTCAAACCAAACAAAAACTGAACCAACAAAATTCAACCCATCCAAACCATTAAGCCGCCATTTTTTAGCCTCTTGTTCTACAAAACTTCTCCATACAACAACCCCAATACGGCTTGTTTTTTGTAACTGCGGCCGTTGTAAAAATCGACCTCCTAAACTTCGTATTTATTATGTATTTGAATGTGAAGAGTTTTTGAATCGAATTGAATTGGGGGAGAAGAAACCCATTTCACCAATCTAAATTTCCACCAAAACCCTCTTTACCAAATGGACATTGCATTCAAATCTTCGAGGTTTCTTCCGCTCAACAAGATACCCAGTACGCCTGCCTTTTCTTCACGTCCTTGTAAGATTTCAGTCTCCCAATCCAAGCGGTTTGCTCCAATGGCTGCTCTTGCAGCTTCCCCTGCTGTTGGACTGTCCGAAACCTTCAACAAATTGAGAGAACAAGGGAAGGTCGCATTGATCCCCTACATCACTGCTGGTGATCCTGATCTTTCCACCACGGCTGAGGCATTGAAGGTGCTTTCGACTTGTGGATCAGATATAATTGAACTTGGTGTTCCCTATTCGGATCCCTTGGCAGATGGTCCTGTTATACAGGCTGCAGCCACTCGGTCCTTGGCTAGGGAGACAAACTTTAATGCAATCATTTCTATGCTGAAGGGGGTGATTCCTGAACTATCTTGTCCAATTTCCCTTTTTACATATTATAACCCAATTCTTAAACGTGGCATTGAGAACTTTATGATGATAATAAAAGATGCTGGTGTGCGAGGGCTTGTTGTTCCAGATGTTCCTCTTGAGGAGACTGAAATTTTAAGAAAGGAAGCTGTAAAGTACAATATTGAACTGGTACTCTTAACTACACCAACCACGCCAAAAGATAGGATGAAAAACATCGCTGAAGCTTCAGAGGGATTTGTTTATCTTGTGAGCTCCGTTGGAGTTACCGGTACCCGCACATCAGTTAGTGGTAGAGTTCAAACTCTCCTTGAGGAAGTTAAAGAGGTTACGAAAAAACCAGTAGCAGTTGGTTTTGGTATATCAAAACCCGAGCATGTGAAACAGGTGGCTGACTGGGGGGCAGATGGTATTATTATTGGTAGTGCTATGGTGAAGCTGTTGGGTGAAGCCCAATCCCCTGAGGAAGGATTGAAGGAGCTTGAAAACTTCACCATATCCTTGAAATCTGCACTCTCTTGATGGCATAACCCAGGCTGTTTCTTTCATCTGTAAACAAGTGCTCAACTTGCACTGTCATCCTAAAATTACACTTCCAATATTTCCCTGTTCTTCTTTTTTGAAACAGTATTTCCCTTTTCTTTTAACAGTGAATAAAATTATCAGCTCGTATTTTCTTCGAAGTTATTTGCCATGGAATATTATGAGAATATTTTCTAGTATTTTCTTGGTAGCCGATGGATGATTGCAAG

Coding sequence (CDS)

ATGGACATTGCATTCAAATCTTCGAGGTTTCTTCCGCTCAACAAGATACCCAGTACGCCTGCCTTTTCTTCACGTCCTTGTAAGATTTCAGTCTCCCAATCCAAGCGGTTTGCTCCAATGGCTGCTCTTGCAGCTTCCCCTGCTGTTGGACTGTCCGAAACCTTCAACAAATTGAGAGAACAAGGGAAGGTCGCATTGATCCCCTACATCACTGCTGGTGATCCTGATCTTTCCACCACGGCTGAGGCATTGAAGGTGCTTTCGACTTGTGGATCAGATATAATTGAACTTGGTGTTCCCTATTCGGATCCCTTGGCAGATGGTCCTGTTATACAGGCTGCAGCCACTCGGTCCTTGGCTAGGGAGACAAACTTTAATGCAATCATTTCTATGCTGAAGGGGGTGATTCCTGAACTATCTTGTCCAATTTCCCTTTTTACATATTATAACCCAATTCTTAAACGTGGCATTGAGAACTTTATGATGATAATAAAAGATGCTGGTGTGCGAGGGCTTGTTGTTCCAGATGTTCCTCTTGAGGAGACTGAAATTTTAAGAAAGGAAGCTGTAAAGTACAATATTGAACTGGTACTCTTAACTACACCAACCACGCCAAAAGATAGGATGAAAAACATCGCTGAAGCTTCAGAGGGATTTGTTTATCTTGTGAGCTCCGTTGGAGTTACCGGTACCCGCACATCAGTTAGTGGTAGAGTTCAAACTCTCCTTGAGGAAGTTAAAGAGGTTACGAAAAAACCAGTAGCAGTTGGTTTTGGTATATCAAAACCCGAGCATGTGAAACAGGTGGCTGACTGGGGGGCAGATGGTATTATTATTGGTAGTGCTATGGTGAAGCTGTTGGGTGAAGCCCAATCCCCTGAGGAAGGATTGAAGGAGCTTGAAAACTTCACCATATCCTTGAAATCTGCACTCTCTTGA

Protein sequence

MDIAFKSSRFLPLNKIPSTPAFSSRPCKISVSQSKRFAPMAALAASPAVGLSETFNKLREQGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRSLARETNFNAIISMLKGVIPELSCPISLFTYYNPILKRGIENFMMIIKDAGVRGLVVPDVPLEETEILRKEAVKYNIELVLLTTPTTPKDRMKNIAEASEGFVYLVSSVGVTGTRTSVSGRVQTLLEEVKEVTKKPVAVGFGISKPEHVKQVADWGADGIIIGSAMVKLLGEAQSPEEGLKELENFTISLKSALS
Homology
BLAST of Clc10G12560 vs. NCBI nr
Match: XP_038906368.1 (tryptophan synthase alpha chain-like [Benincasa hispida])

HSP 1 Score: 567.0 bits (1460), Expect = 1.0e-157
Identity = 297/312 (95.19%), Postives = 301/312 (96.47%), Query Frame = 0

Query: 1   MDIAFKSSRFLPLNKIPSTPAFSSRPCKISVSQSKRFAPMAALAASPAVGLSETFNKLRE 60
           M IAFKSS F PLNKIPS+P FS RPCKISV +SKRFAPMA LAASP VGLSETFNKLRE
Sbjct: 1   MAIAFKSSSFRPLNKIPSSPIFSPRPCKISVFKSKRFAPMATLAASPVVGLSETFNKLRE 60

Query: 61  QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRSLA 120
           QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRSLA
Sbjct: 61  QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRSLA 120

Query: 121 RETNFNAIISMLKGVIPELSCPISLFTYYNPILKRGIENFMMIIKDAGVRGLVVPDVPLE 180
           RETNFNAIISMLKGVIPELSCPISLFTYYN ILKRGIENF+MIIKDAGVRGLVVPDVPLE
Sbjct: 121 RETNFNAIISMLKGVIPELSCPISLFTYYNLILKRGIENFVMIIKDAGVRGLVVPDVPLE 180

Query: 181 ETEILRKEAVKYNIELVLLTTPTTPKDRMKNIAEASEGFVYLVSSVGVTGTRTSVSGRVQ 240
           ETEILRKEAVKYNIELVLLTTPTTPKDRMK+IAEASEGFVYLVSSVGVTGTRTSVSGRVQ
Sbjct: 181 ETEILRKEAVKYNIELVLLTTPTTPKDRMKDIAEASEGFVYLVSSVGVTGTRTSVSGRVQ 240

Query: 241 TLLEEVKEVTKKPVAVGFGISKPEHVKQVADWGADGIIIGSAMVKLLGEAQSPEEGLKEL 300
           TLLEEVKEVT KPVAVGFGISKPEHVKQVADWGADGIIIGSAMVKLLGEAQSPEEGLKEL
Sbjct: 241 TLLEEVKEVTDKPVAVGFGISKPEHVKQVADWGADGIIIGSAMVKLLGEAQSPEEGLKEL 300

Query: 301 ENFTISLKSALS 313
           ENFT SLKSALS
Sbjct: 301 ENFTKSLKSALS 312

BLAST of Clc10G12560 vs. NCBI nr
Match: XP_008465320.1 (PREDICTED: tryptophan synthase alpha chain-like isoform X1 [Cucumis melo] >KAA0051282.1 tryptophan synthase alpha chain-like isoform X1 [Cucumis melo var. makuwa] >TYK29979.1 tryptophan synthase alpha chain-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 563.1 bits (1450), Expect = 1.5e-156
Identity = 291/312 (93.27%), Postives = 300/312 (96.15%), Query Frame = 0

Query: 1   MDIAFKSSRFLPLNKIPSTPAFSSRPCKISVSQSKRFAPMAALAASPAVGLSETFNKLRE 60
           MDIAFK SR LPLNKIP+T  FS RPCKISVSQ+KRFAPMAALAA P VGLSETF KLRE
Sbjct: 1   MDIAFKPSRILPLNKIPNTLIFSPRPCKISVSQTKRFAPMAALAAYPVVGLSETFKKLRE 60

Query: 61  QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRSLA 120
           QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRSLA
Sbjct: 61  QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRSLA 120

Query: 121 RETNFNAIISMLKGVIPELSCPISLFTYYNPILKRGIENFMMIIKDAGVRGLVVPDVPLE 180
           RETNFNAIISMLKGVIPELSCPISLFTYYNPILKRG+ENFMMIIKD GVRGLVVPDVPLE
Sbjct: 121 RETNFNAIISMLKGVIPELSCPISLFTYYNPILKRGVENFMMIIKDTGVRGLVVPDVPLE 180

Query: 181 ETEILRKEAVKYNIELVLLTTPTTPKDRMKNIAEASEGFVYLVSSVGVTGTRTSVSGRVQ 240
           ETE+LRKEAVK+NIELVLLTTPTTPK+RMK I EASEGFVYLVSS+GVTGTRTSVSGRVQ
Sbjct: 181 ETEVLRKEAVKHNIELVLLTTPTTPKERMKKIVEASEGFVYLVSSIGVTGTRTSVSGRVQ 240

Query: 241 TLLEEVKEVTKKPVAVGFGISKPEHVKQVADWGADGIIIGSAMVKLLGEAQSPEEGLKEL 300
           TLLEEVKEVT+KPVAVGFGISKPEHVKQVA+WGADGIIIGSAMVKLLGEAQSPEEGLKEL
Sbjct: 241 TLLEEVKEVTEKPVAVGFGISKPEHVKQVAEWGADGIIIGSAMVKLLGEAQSPEEGLKEL 300

Query: 301 ENFTISLKSALS 313
           ENFT SLKSALS
Sbjct: 301 ENFTKSLKSALS 312

BLAST of Clc10G12560 vs. NCBI nr
Match: XP_004141207.1 (tryptophan synthase alpha chain [Cucumis sativus] >KGN59758.1 hypothetical protein Csa_002167 [Cucumis sativus])

HSP 1 Score: 557.4 bits (1435), Expect = 8.0e-155
Identity = 290/312 (92.95%), Postives = 298/312 (95.51%), Query Frame = 0

Query: 1   MDIAFKSSRFLPLNKIPSTPAFSSRPCKISVSQSKRFAPMAALAASPAVGLSETFNKLRE 60
           MDIAFKSSR LPLNKIP+T  FS RPCKISVSQSKRFAPMAALAA P VGLSETF  LRE
Sbjct: 1   MDIAFKSSRILPLNKIPNTLIFSPRPCKISVSQSKRFAPMAALAAYPVVGLSETFKNLRE 60

Query: 61  QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRSLA 120
           QGKVALIPYITAGDPDLSTTAEALKVLS CGSDIIELGVPYSDPLADGPVIQAAATRSLA
Sbjct: 61  QGKVALIPYITAGDPDLSTTAEALKVLSKCGSDIIELGVPYSDPLADGPVIQAAATRSLA 120

Query: 121 RETNFNAIISMLKGVIPELSCPISLFTYYNPILKRGIENFMMIIKDAGVRGLVVPDVPLE 180
           RETNFNAIISMLKGVIPELS PISLFTYYNPILKRG+ENFMMIIKD GVRGLVVPDVPLE
Sbjct: 121 RETNFNAIISMLKGVIPELSRPISLFTYYNPILKRGVENFMMIIKDTGVRGLVVPDVPLE 180

Query: 181 ETEILRKEAVKYNIELVLLTTPTTPKDRMKNIAEASEGFVYLVSSVGVTGTRTSVSGRVQ 240
           ETE+LRKEAVK+NIELVLLTTPTTPK+RMKNI EASEGFVYLVSS+GVTGTRTSVS RVQ
Sbjct: 181 ETEVLRKEAVKHNIELVLLTTPTTPKERMKNIVEASEGFVYLVSSIGVTGTRTSVSSRVQ 240

Query: 241 TLLEEVKEVTKKPVAVGFGISKPEHVKQVADWGADGIIIGSAMVKLLGEAQSPEEGLKEL 300
           TLLEEVKEVT+KPVAVGFGISKPEHVKQVA+WGADGIIIGSAMVKLLGEAQSPEEGLKEL
Sbjct: 241 TLLEEVKEVTEKPVAVGFGISKPEHVKQVAEWGADGIIIGSAMVKLLGEAQSPEEGLKEL 300

Query: 301 ENFTISLKSALS 313
           ENFT SLKSALS
Sbjct: 301 ENFTRSLKSALS 312

BLAST of Clc10G12560 vs. NCBI nr
Match: XP_022932801.1 (tryptophan synthase alpha chain-like [Cucurbita moschata])

HSP 1 Score: 552.0 bits (1421), Expect = 3.3e-153
Identity = 283/312 (90.71%), Postives = 297/312 (95.19%), Query Frame = 0

Query: 1   MDIAFKSSRFLPLNKIPSTPAFSSRPCKISVSQSKRFAPMAALAASPAVGLSETFNKLRE 60
           MDIAFKSSRFLPLNKIP+T   S RPC ISV QSKRFAP+AAL ASPA GLSETFNKLRE
Sbjct: 1   MDIAFKSSRFLPLNKIPTTRIVSPRPCTISVFQSKRFAPIAALTASPAAGLSETFNKLRE 60

Query: 61  QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRSLA 120
           QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATR+LA
Sbjct: 61  QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRALA 120

Query: 121 RETNFNAIISMLKGVIPELSCPISLFTYYNPILKRGIENFMMIIKDAGVRGLVVPDVPLE 180
           RETNFNAIISMLKGVIPELSCP+SLF+YYNPILKRGIENFMM I+DAGVRGLVVPDVPLE
Sbjct: 121 RETNFNAIISMLKGVIPELSCPVSLFSYYNPILKRGIENFMMTIRDAGVRGLVVPDVPLE 180

Query: 181 ETEILRKEAVKYNIELVLLTTPTTPKDRMKNIAEASEGFVYLVSSVGVTGTRTSVSGRVQ 240
           ET +LR+EAVKYNIELVLLTTPTTPKDRMKNI EASEGFVYLVSSVGVTG RTSVSGRVQ
Sbjct: 181 ETAVLREEAVKYNIELVLLTTPTTPKDRMKNIVEASEGFVYLVSSVGVTGARTSVSGRVQ 240

Query: 241 TLLEEVKEVTKKPVAVGFGISKPEHVKQVADWGADGIIIGSAMVKLLGEAQSPEEGLKEL 300
           +LLEE+KEVT+KPVAVGFGISKPEHVKQVA WGADG+I+GSAMVKLLGEAQSPEEGLKEL
Sbjct: 241 SLLEEIKEVTEKPVAVGFGISKPEHVKQVAGWGADGVIVGSAMVKLLGEAQSPEEGLKEL 300

Query: 301 ENFTISLKSALS 313
           E FT SL+SALS
Sbjct: 301 ETFTKSLRSALS 312

BLAST of Clc10G12560 vs. NCBI nr
Match: XP_023528646.1 (tryptophan synthase alpha chain-like [Cucurbita pepo subsp. pepo] >XP_023528647.1 tryptophan synthase alpha chain-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 551.6 bits (1420), Expect = 4.4e-153
Identity = 283/312 (90.71%), Postives = 296/312 (94.87%), Query Frame = 0

Query: 1   MDIAFKSSRFLPLNKIPSTPAFSSRPCKISVSQSKRFAPMAALAASPAVGLSETFNKLRE 60
           MDIAFKSSRFLPLNKIP+T   S RPC ISV QSKRFAP+AAL ASPA GLSETFNKLRE
Sbjct: 1   MDIAFKSSRFLPLNKIPTTRIVSPRPCTISVFQSKRFAPIAALTASPAAGLSETFNKLRE 60

Query: 61  QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRSLA 120
           QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATR+L 
Sbjct: 61  QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRALV 120

Query: 121 RETNFNAIISMLKGVIPELSCPISLFTYYNPILKRGIENFMMIIKDAGVRGLVVPDVPLE 180
           RETNFNAIISMLKGVIPELSCP+SLF+YYNPILKRGIENFMM I+DAGVRGLVVPDVPLE
Sbjct: 121 RETNFNAIISMLKGVIPELSCPVSLFSYYNPILKRGIENFMMTIRDAGVRGLVVPDVPLE 180

Query: 181 ETEILRKEAVKYNIELVLLTTPTTPKDRMKNIAEASEGFVYLVSSVGVTGTRTSVSGRVQ 240
           ET +LR+EAVKYNIELVLLTTPTTPKDRMKNI EASEGFVYLVSSVGVTG RTSVSGRVQ
Sbjct: 181 ETAVLREEAVKYNIELVLLTTPTTPKDRMKNIVEASEGFVYLVSSVGVTGARTSVSGRVQ 240

Query: 241 TLLEEVKEVTKKPVAVGFGISKPEHVKQVADWGADGIIIGSAMVKLLGEAQSPEEGLKEL 300
           +LLEE+KEVT+KPVAVGFGISKPEHVKQVA WGADG+I+GSAMVKLLGEAQSPEEGLKEL
Sbjct: 241 SLLEEIKEVTEKPVAVGFGISKPEHVKQVAGWGADGVIVGSAMVKLLGEAQSPEEGLKEL 300

Query: 301 ENFTISLKSALS 313
           E FT SLKSALS
Sbjct: 301 ETFTKSLKSALS 312

BLAST of Clc10G12560 vs. ExPASy Swiss-Prot
Match: O22765 (Tryptophan synthase alpha chain OS=Arabidopsis thaliana OX=3702 GN=TRPA1 PE=1 SV=2)

HSP 1 Score: 423.3 bits (1087), Expect = 2.4e-117
Identity = 211/266 (79.32%), Postives = 241/266 (90.60%), Query Frame = 0

Query: 46  SPAVGLSETFNKLREQGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPL 105
           S  VGLSETF +L+ QGKVALIPYITAGDPDLSTTA+ALKVL +CGSDIIELGVPYSDPL
Sbjct: 8   SSTVGLSETFARLKSQGKVALIPYITAGDPDLSTTAKALKVLDSCGSDIIELGVPYSDPL 67

Query: 106 ADGPVIQAAATRSLARETNFNAIISMLKGVIPELSCPISLFTYYNPILKRGIENFMMIIK 165
           ADGP IQAAA RSL + TNFN+IISMLK VIP+LSCPI+LFTYYNPIL+RG+EN+M +IK
Sbjct: 68  ADGPAIQAAARRSLLKGTNFNSIISMLKEVIPQLSCPIALFTYYNPILRRGVENYMTVIK 127

Query: 166 DAGVRGLVVPDVPLEETEILRKEAVKYNIELVLLTTPTTPKDRMKNIAEASEGFVYLVSS 225
           +AGV GL+VPDVPLEETE LR EA K+ IELVLLTTPTTPK+RM  I EASEGF+YLVSS
Sbjct: 128 NAGVHGLLVPDVPLEETETLRNEARKHQIELVLLTTPTTPKERMNAIVEASEGFIYLVSS 187

Query: 226 VGVTGTRTSVSGRVQTLLEEVKEVTKKPVAVGFGISKPEHVKQVADWGADGIIIGSAMVK 285
           VGVTGTR SV+ +VQ+LL+++KE T KPVAVGFGISKPEHVKQVA+WGADG+I+GSAMVK
Sbjct: 188 VGVTGTRESVNEKVQSLLQQIKEATSKPVAVGFGISKPEHVKQVAEWGADGVIVGSAMVK 247

Query: 286 LLGEAQSPEEGLKELENFTISLKSAL 312
           +LGE++SPE+GLKELE FT SLKSAL
Sbjct: 248 ILGESESPEQGLKELEFFTKSLKSAL 273

BLAST of Clc10G12560 vs. ExPASy Swiss-Prot
Match: Q42529 (Tryptophan synthase alpha chain, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TSA1 PE=1 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 5.2e-117
Identity = 221/312 (70.83%), Postives = 257/312 (82.37%), Query Frame = 0

Query: 1   MDIAFKSSRFLPLNKIPSTPAFSSRPCKISVSQSKRFAPMAALA-ASPAVGLSETFNKLR 60
           M IAFKS  F  L    S   F       S    KRF PMA+L+ +SP +GL++TF +L+
Sbjct: 1   MAIAFKSGVFF-LQSPKSQIGFRHSSPPDSSLSFKRFTPMASLSTSSPTLGLADTFTQLK 60

Query: 61  EQGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRSL 120
           +QGKVA IPYITAGDPDLSTTAEALKVL  CGSDIIELGVPYSDPLADGPVIQAAATRSL
Sbjct: 61  KQGKVAFIPYITAGDPDLSTTAEALKVLDACGSDIIELGVPYSDPLADGPVIQAAATRSL 120

Query: 121 ARETNFNAIISMLKGVIPELSCPISLFTYYNPILKRGIENFMMIIKDAGVRGLVVPDVPL 180
            R TN ++I+ ML  V+P++SCPISLFTYYNPILKRG+  FM  I+  GV+GLVVPDVPL
Sbjct: 121 ERGTNLDSILEMLDKVVPQISCPISLFTYYNPILKRGLGKFMSSIRAVGVQGLVVPDVPL 180

Query: 181 EETEILRKEAVKYNIELVLLTTPTTPKDRMKNIAEASEGFVYLVSSVGVTGTRTSVSGRV 240
           EETE+LRKEA+  +IELVLLTTPTTP +RMK I +ASEGF+YLVSS+GVTG R+SVSG+V
Sbjct: 181 EETEMLRKEALNNDIELVLLTTPTTPTERMKLIVDASEGFIYLVSSIGVTGARSSVSGKV 240

Query: 241 QTLLEEVKEVTKKPVAVGFGISKPEHVKQVADWGADGIIIGSAMVKLLGEAQSPEEGLKE 300
           Q+LL+++KE T KPVAVGFGISKPEHVKQ+A WGADG+I+GSAMVKLLG+A+SP EGLKE
Sbjct: 241 QSLLKDIKEATDKPVAVGFGISKPEHVKQIAGWGADGVIVGSAMVKLLGDAKSPTEGLKE 300

Query: 301 LENFTISLKSAL 312
           LE  T SLKSAL
Sbjct: 301 LEKLTKSLKSAL 311

BLAST of Clc10G12560 vs. ExPASy Swiss-Prot
Match: P42390 (Indole-3-glycerol phosphate lyase, chloroplastic OS=Zea mays OX=4577 GN=BX1 PE=1 SV=2)

HSP 1 Score: 318.2 bits (814), Expect = 1.1e-85
Identity = 164/296 (55.41%), Postives = 212/296 (71.62%), Query Frame = 0

Query: 16  IPSTPAFSSRPCKISVSQSKRFAPMAALAASPAVGLSETFNKLREQGKVALIPYITAGDP 75
           +P+ P  +  P  +   Q+      AA A   +  +S+T   L  +GK A IPYITAGDP
Sbjct: 61  VPAAPPQAPAPAPVPPKQA------AAPAERRSRPVSDTMAALMAKGKTAFIPYITAGDP 120

Query: 76  DLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRSLARETNFNAIISMLKGV 135
           DL+TTAEAL++L  CG+D+IELGVP SDP  DGP+IQA+  R+LA  T  +A++ ML+ V
Sbjct: 121 DLATTAEALRLLDGCGADVIELGVPCSDPYIDGPIIQASVARALASGTTMDAVLEMLREV 180

Query: 136 IPELSCPISLFTYYNPILKRGIENFMMIIKDAGVRGLVVPDVPLEETEILRKEAVKYNIE 195
            PELSCP+ L +YY PI+ R +      +K+AGV GL+VPD+P      L  EA   N+E
Sbjct: 181 TPELSCPVVLLSYYKPIMSRSLAE----MKEAGVHGLIVPDLPYVAAHSLWSEAKNNNLE 240

Query: 196 LVLLTTPTTPKDRMKNIAEASEGFVYLVSSVGVTGTRTSVSGRVQTLLEEVKEVTKKPVA 255
           LVLLTTP  P+DRMK I +ASEGFVYLVS  GVTG R +V+ RV++L++EVK+VT KPVA
Sbjct: 241 LVLLTTPAIPEDRMKEITKASEGFVYLVSVNGVTGPRANVNPRVESLIQEVKKVTNKPVA 300

Query: 256 VGFGISKPEHVKQVADWGADGIIIGSAMVKLLGEAQSPEEGLKELENFTISLKSAL 312
           VGFGISKPEHVKQ+A WGADG+IIGSAMV+ LGEA SP++GL+ LE +   +K+AL
Sbjct: 301 VGFGISKPEHVKQIAQWGADGVIIGSAMVRQLGEAASPKQGLRRLEEYARGMKNAL 346

BLAST of Clc10G12560 vs. ExPASy Swiss-Prot
Match: B8HPH2 (Tryptophan synthase alpha chain OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) OX=395961 GN=trpA PE=3 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 8.7e-80
Identity = 149/264 (56.44%), Postives = 194/264 (73.48%), Query Frame = 0

Query: 49  VGLSETFNKLREQGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADG 108
           V +S  F+ LR++ + ALIP+ITAGDP L  TA+AL+VL   G+D+IELGVPYSDPLADG
Sbjct: 2   VSVSTCFSALRDRAQCALIPFITAGDPSLEITAKALQVLDQQGADLIELGVPYSDPLADG 61

Query: 109 PVIQAAATRSLARETNFNAIISMLKGVIPELSCPISLFTYYNPILKRGIENFMMIIKDAG 168
           P IQAAATR+L + T  +A++ M+  V P L  P+ LFTYYNPI  RG+E F+  +  AG
Sbjct: 62  PTIQAAATRALQKGTRLDAVLEMISHVAPNLRSPLILFTYYNPIFHRGVEPFLQQVAQAG 121

Query: 169 VRGLVVPDVPLEETEILRKEAVKYNIELVLLTTPTTPKDRMKNIAEASEGFVYLVSSVGV 228
           V+GLVVPD+PLEE + +  +A    IEL LL  PTTP+ R+  IAE S+GF+YLVS+ GV
Sbjct: 122 VQGLVVPDLPLEEADTVLTQAAAVGIELTLLVAPTTPRSRIAAIAERSQGFIYLVSTTGV 181

Query: 229 TGTRTSVSGRVQTLLEEVKEVTKKPVAVGFGISKPEHVKQVADWGADGIIIGSAMVKLLG 288
           TG R+ V GRV  LL E+++VT KP+ VGFGIS+PEH +QV +WGAD  I+GSA VK L 
Sbjct: 182 TGMRSKVEGRVHELLLELQQVTDKPIGVGFGISQPEHARQVMEWGADAAIVGSAFVKRLA 241

Query: 289 EAQSPEEGLKELENFTISLKSALS 313
           E  +PE+GL  + +F  SLK+AL+
Sbjct: 242 EG-TPEQGLAAIADFCRSLKTALT 264

BLAST of Clc10G12560 vs. ExPASy Swiss-Prot
Match: B0C6F8 (Tryptophan synthase alpha chain OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=trpA PE=3 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 1.9e-79
Identity = 148/261 (56.70%), Postives = 193/261 (73.95%), Query Frame = 0

Query: 51  LSETFNKLREQGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPV 110
           +S+ F +LR++G+ ALIP++TAGDPDL+TTA AL+ L   G+D+IELGVPYSDPLADGPV
Sbjct: 4   VSDCFAQLRDRGQCALIPFLTAGDPDLATTASALRQLDASGADLIELGVPYSDPLADGPV 63

Query: 111 IQAAATRSLARETNFNAIISMLKGVIPELSCPISLFTYYNPILKRGIENFMMIIKDAGVR 170
           IQAAATR+L R T  + ++ M+  + PE+  PI LFTYYNPI  RG+  F+  I  AGVR
Sbjct: 64  IQAAATRALQRGTRLDQVLEMVTELSPEIRAPIILFTYYNPIYHRGVAEFLQQIAKAGVR 123

Query: 171 GLVVPDVPLEETEILRKEAVKYNIELVLLTTPTTPKDRMKNIAEASEGFVYLVSSVGVTG 230
           GLVVPD+PLEE+E L ++A    IE+ LL  PT+ K+R++ IA  S+GF+YLVS+ GVTG
Sbjct: 124 GLVVPDLPLEESENLLQQAADLGIEVTLLVAPTSSKERIEKIALRSQGFIYLVSTTGVTG 183

Query: 231 TRTSVSGRVQTLLEEVKEVTKKPVAVGFGISKPEHVKQVADWGADGIIIGSAMVKLLGEA 290
            RT V  RVQ L+ ++++VT KP+ VGFGIS+ EH +QV DWGAD  I+GSA V  L E 
Sbjct: 184 MRTKVENRVQDLIADLRQVTDKPIGVGFGISRTEHARQVMDWGADAAIVGSAFVNRLSEG 243

Query: 291 QSPEEGLKELENFTISLKSAL 312
            SP +GL  +  F  SLKS+L
Sbjct: 244 -SPSQGLSAISTFCRSLKSSL 263

BLAST of Clc10G12560 vs. ExPASy TrEMBL
Match: A0A5D3E2H9 (Tryptophan synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold734G00080 PE=3 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 7.0e-157
Identity = 291/312 (93.27%), Postives = 300/312 (96.15%), Query Frame = 0

Query: 1   MDIAFKSSRFLPLNKIPSTPAFSSRPCKISVSQSKRFAPMAALAASPAVGLSETFNKLRE 60
           MDIAFK SR LPLNKIP+T  FS RPCKISVSQ+KRFAPMAALAA P VGLSETF KLRE
Sbjct: 1   MDIAFKPSRILPLNKIPNTLIFSPRPCKISVSQTKRFAPMAALAAYPVVGLSETFKKLRE 60

Query: 61  QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRSLA 120
           QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRSLA
Sbjct: 61  QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRSLA 120

Query: 121 RETNFNAIISMLKGVIPELSCPISLFTYYNPILKRGIENFMMIIKDAGVRGLVVPDVPLE 180
           RETNFNAIISMLKGVIPELSCPISLFTYYNPILKRG+ENFMMIIKD GVRGLVVPDVPLE
Sbjct: 121 RETNFNAIISMLKGVIPELSCPISLFTYYNPILKRGVENFMMIIKDTGVRGLVVPDVPLE 180

Query: 181 ETEILRKEAVKYNIELVLLTTPTTPKDRMKNIAEASEGFVYLVSSVGVTGTRTSVSGRVQ 240
           ETE+LRKEAVK+NIELVLLTTPTTPK+RMK I EASEGFVYLVSS+GVTGTRTSVSGRVQ
Sbjct: 181 ETEVLRKEAVKHNIELVLLTTPTTPKERMKKIVEASEGFVYLVSSIGVTGTRTSVSGRVQ 240

Query: 241 TLLEEVKEVTKKPVAVGFGISKPEHVKQVADWGADGIIIGSAMVKLLGEAQSPEEGLKEL 300
           TLLEEVKEVT+KPVAVGFGISKPEHVKQVA+WGADGIIIGSAMVKLLGEAQSPEEGLKEL
Sbjct: 241 TLLEEVKEVTEKPVAVGFGISKPEHVKQVAEWGADGIIIGSAMVKLLGEAQSPEEGLKEL 300

Query: 301 ENFTISLKSALS 313
           ENFT SLKSALS
Sbjct: 301 ENFTKSLKSALS 312

BLAST of Clc10G12560 vs. ExPASy TrEMBL
Match: A0A1S3CNL8 (Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103502969 PE=3 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 7.0e-157
Identity = 291/312 (93.27%), Postives = 300/312 (96.15%), Query Frame = 0

Query: 1   MDIAFKSSRFLPLNKIPSTPAFSSRPCKISVSQSKRFAPMAALAASPAVGLSETFNKLRE 60
           MDIAFK SR LPLNKIP+T  FS RPCKISVSQ+KRFAPMAALAA P VGLSETF KLRE
Sbjct: 1   MDIAFKPSRILPLNKIPNTLIFSPRPCKISVSQTKRFAPMAALAAYPVVGLSETFKKLRE 60

Query: 61  QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRSLA 120
           QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRSLA
Sbjct: 61  QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRSLA 120

Query: 121 RETNFNAIISMLKGVIPELSCPISLFTYYNPILKRGIENFMMIIKDAGVRGLVVPDVPLE 180
           RETNFNAIISMLKGVIPELSCPISLFTYYNPILKRG+ENFMMIIKD GVRGLVVPDVPLE
Sbjct: 121 RETNFNAIISMLKGVIPELSCPISLFTYYNPILKRGVENFMMIIKDTGVRGLVVPDVPLE 180

Query: 181 ETEILRKEAVKYNIELVLLTTPTTPKDRMKNIAEASEGFVYLVSSVGVTGTRTSVSGRVQ 240
           ETE+LRKEAVK+NIELVLLTTPTTPK+RMK I EASEGFVYLVSS+GVTGTRTSVSGRVQ
Sbjct: 181 ETEVLRKEAVKHNIELVLLTTPTTPKERMKKIVEASEGFVYLVSSIGVTGTRTSVSGRVQ 240

Query: 241 TLLEEVKEVTKKPVAVGFGISKPEHVKQVADWGADGIIIGSAMVKLLGEAQSPEEGLKEL 300
           TLLEEVKEVT+KPVAVGFGISKPEHVKQVA+WGADGIIIGSAMVKLLGEAQSPEEGLKEL
Sbjct: 241 TLLEEVKEVTEKPVAVGFGISKPEHVKQVAEWGADGIIIGSAMVKLLGEAQSPEEGLKEL 300

Query: 301 ENFTISLKSALS 313
           ENFT SLKSALS
Sbjct: 301 ENFTKSLKSALS 312

BLAST of Clc10G12560 vs. ExPASy TrEMBL
Match: A0A0A0LD81 (Tryptophan synthase OS=Cucumis sativus OX=3659 GN=Csa_3G843770 PE=3 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 3.9e-155
Identity = 290/312 (92.95%), Postives = 298/312 (95.51%), Query Frame = 0

Query: 1   MDIAFKSSRFLPLNKIPSTPAFSSRPCKISVSQSKRFAPMAALAASPAVGLSETFNKLRE 60
           MDIAFKSSR LPLNKIP+T  FS RPCKISVSQSKRFAPMAALAA P VGLSETF  LRE
Sbjct: 1   MDIAFKSSRILPLNKIPNTLIFSPRPCKISVSQSKRFAPMAALAAYPVVGLSETFKNLRE 60

Query: 61  QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRSLA 120
           QGKVALIPYITAGDPDLSTTAEALKVLS CGSDIIELGVPYSDPLADGPVIQAAATRSLA
Sbjct: 61  QGKVALIPYITAGDPDLSTTAEALKVLSKCGSDIIELGVPYSDPLADGPVIQAAATRSLA 120

Query: 121 RETNFNAIISMLKGVIPELSCPISLFTYYNPILKRGIENFMMIIKDAGVRGLVVPDVPLE 180
           RETNFNAIISMLKGVIPELS PISLFTYYNPILKRG+ENFMMIIKD GVRGLVVPDVPLE
Sbjct: 121 RETNFNAIISMLKGVIPELSRPISLFTYYNPILKRGVENFMMIIKDTGVRGLVVPDVPLE 180

Query: 181 ETEILRKEAVKYNIELVLLTTPTTPKDRMKNIAEASEGFVYLVSSVGVTGTRTSVSGRVQ 240
           ETE+LRKEAVK+NIELVLLTTPTTPK+RMKNI EASEGFVYLVSS+GVTGTRTSVS RVQ
Sbjct: 181 ETEVLRKEAVKHNIELVLLTTPTTPKERMKNIVEASEGFVYLVSSIGVTGTRTSVSSRVQ 240

Query: 241 TLLEEVKEVTKKPVAVGFGISKPEHVKQVADWGADGIIIGSAMVKLLGEAQSPEEGLKEL 300
           TLLEEVKEVT+KPVAVGFGISKPEHVKQVA+WGADGIIIGSAMVKLLGEAQSPEEGLKEL
Sbjct: 241 TLLEEVKEVTEKPVAVGFGISKPEHVKQVAEWGADGIIIGSAMVKLLGEAQSPEEGLKEL 300

Query: 301 ENFTISLKSALS 313
           ENFT SLKSALS
Sbjct: 301 ENFTRSLKSALS 312

BLAST of Clc10G12560 vs. ExPASy TrEMBL
Match: A0A6J1EXE2 (Tryptophan synthase OS=Cucurbita moschata OX=3662 GN=LOC111439260 PE=3 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 1.6e-153
Identity = 283/312 (90.71%), Postives = 297/312 (95.19%), Query Frame = 0

Query: 1   MDIAFKSSRFLPLNKIPSTPAFSSRPCKISVSQSKRFAPMAALAASPAVGLSETFNKLRE 60
           MDIAFKSSRFLPLNKIP+T   S RPC ISV QSKRFAP+AAL ASPA GLSETFNKLRE
Sbjct: 1   MDIAFKSSRFLPLNKIPTTRIVSPRPCTISVFQSKRFAPIAALTASPAAGLSETFNKLRE 60

Query: 61  QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRSLA 120
           QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATR+LA
Sbjct: 61  QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRALA 120

Query: 121 RETNFNAIISMLKGVIPELSCPISLFTYYNPILKRGIENFMMIIKDAGVRGLVVPDVPLE 180
           RETNFNAIISMLKGVIPELSCP+SLF+YYNPILKRGIENFMM I+DAGVRGLVVPDVPLE
Sbjct: 121 RETNFNAIISMLKGVIPELSCPVSLFSYYNPILKRGIENFMMTIRDAGVRGLVVPDVPLE 180

Query: 181 ETEILRKEAVKYNIELVLLTTPTTPKDRMKNIAEASEGFVYLVSSVGVTGTRTSVSGRVQ 240
           ET +LR+EAVKYNIELVLLTTPTTPKDRMKNI EASEGFVYLVSSVGVTG RTSVSGRVQ
Sbjct: 181 ETAVLREEAVKYNIELVLLTTPTTPKDRMKNIVEASEGFVYLVSSVGVTGARTSVSGRVQ 240

Query: 241 TLLEEVKEVTKKPVAVGFGISKPEHVKQVADWGADGIIIGSAMVKLLGEAQSPEEGLKEL 300
           +LLEE+KEVT+KPVAVGFGISKPEHVKQVA WGADG+I+GSAMVKLLGEAQSPEEGLKEL
Sbjct: 241 SLLEEIKEVTEKPVAVGFGISKPEHVKQVAGWGADGVIVGSAMVKLLGEAQSPEEGLKEL 300

Query: 301 ENFTISLKSALS 313
           E FT SL+SALS
Sbjct: 301 ETFTKSLRSALS 312

BLAST of Clc10G12560 vs. ExPASy TrEMBL
Match: A0A1S4E560 (Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103502969 PE=3 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 2.1e-153
Identity = 288/312 (92.31%), Postives = 297/312 (95.19%), Query Frame = 0

Query: 1   MDIAFKSSRFLPLNKIPSTPAFSSRPCKISVSQSKRFAPMAALAASPAVGLSETFNKLRE 60
           MDIAFK SR LPLNKIP+T  FS RPCKISVSQ+KRFAPMAALAA P VGLSETF KLRE
Sbjct: 1   MDIAFKPSRILPLNKIPNTLIFSPRPCKISVSQTKRFAPMAALAAYPVVGLSETFKKLRE 60

Query: 61  QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRSLA 120
           QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRSLA
Sbjct: 61  QGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRSLA 120

Query: 121 RETNFNAIISMLKGVIPELSCPISLFTYYNPILKRGIENFMMIIKDAGVRGLVVPDVPLE 180
           RETNFNAIISMLKGVIPELSCPISLFTYYNPILKRG+ENFMMIIKD    GLVVPDVPLE
Sbjct: 121 RETNFNAIISMLKGVIPELSCPISLFTYYNPILKRGVENFMMIIKDT---GLVVPDVPLE 180

Query: 181 ETEILRKEAVKYNIELVLLTTPTTPKDRMKNIAEASEGFVYLVSSVGVTGTRTSVSGRVQ 240
           ETE+LRKEAVK+NIELVLLTTPTTPK+RMK I EASEGFVYLVSS+GVTGTRTSVSGRVQ
Sbjct: 181 ETEVLRKEAVKHNIELVLLTTPTTPKERMKKIVEASEGFVYLVSSIGVTGTRTSVSGRVQ 240

Query: 241 TLLEEVKEVTKKPVAVGFGISKPEHVKQVADWGADGIIIGSAMVKLLGEAQSPEEGLKEL 300
           TLLEEVKEVT+KPVAVGFGISKPEHVKQVA+WGADGIIIGSAMVKLLGEAQSPEEGLKEL
Sbjct: 241 TLLEEVKEVTEKPVAVGFGISKPEHVKQVAEWGADGIIIGSAMVKLLGEAQSPEEGLKEL 300

Query: 301 ENFTISLKSALS 313
           ENFT SLKSALS
Sbjct: 301 ENFTKSLKSALS 309

BLAST of Clc10G12560 vs. TAIR 10
Match: AT4G02610.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 423.3 bits (1087), Expect = 1.7e-118
Identity = 211/266 (79.32%), Postives = 241/266 (90.60%), Query Frame = 0

Query: 46  SPAVGLSETFNKLREQGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPL 105
           S  VGLSETF +L+ QGKVALIPYITAGDPDLSTTA+ALKVL +CGSDIIELGVPYSDPL
Sbjct: 8   SSTVGLSETFARLKSQGKVALIPYITAGDPDLSTTAKALKVLDSCGSDIIELGVPYSDPL 67

Query: 106 ADGPVIQAAATRSLARETNFNAIISMLKGVIPELSCPISLFTYYNPILKRGIENFMMIIK 165
           ADGP IQAAA RSL + TNFN+IISMLK VIP+LSCPI+LFTYYNPIL+RG+EN+M +IK
Sbjct: 68  ADGPAIQAAARRSLLKGTNFNSIISMLKEVIPQLSCPIALFTYYNPILRRGVENYMTVIK 127

Query: 166 DAGVRGLVVPDVPLEETEILRKEAVKYNIELVLLTTPTTPKDRMKNIAEASEGFVYLVSS 225
           +AGV GL+VPDVPLEETE LR EA K+ IELVLLTTPTTPK+RM  I EASEGF+YLVSS
Sbjct: 128 NAGVHGLLVPDVPLEETETLRNEARKHQIELVLLTTPTTPKERMNAIVEASEGFIYLVSS 187

Query: 226 VGVTGTRTSVSGRVQTLLEEVKEVTKKPVAVGFGISKPEHVKQVADWGADGIIIGSAMVK 285
           VGVTGTR SV+ +VQ+LL+++KE T KPVAVGFGISKPEHVKQVA+WGADG+I+GSAMVK
Sbjct: 188 VGVTGTRESVNEKVQSLLQQIKEATSKPVAVGFGISKPEHVKQVAEWGADGVIVGSAMVK 247

Query: 286 LLGEAQSPEEGLKELENFTISLKSAL 312
           +LGE++SPE+GLKELE FT SLKSAL
Sbjct: 248 ILGESESPEQGLKELEFFTKSLKSAL 273

BLAST of Clc10G12560 vs. TAIR 10
Match: AT3G54640.1 (tryptophan synthase alpha chain )

HSP 1 Score: 422.2 bits (1084), Expect = 3.7e-118
Identity = 221/312 (70.83%), Postives = 257/312 (82.37%), Query Frame = 0

Query: 1   MDIAFKSSRFLPLNKIPSTPAFSSRPCKISVSQSKRFAPMAALA-ASPAVGLSETFNKLR 60
           M IAFKS  F  L    S   F       S    KRF PMA+L+ +SP +GL++TF +L+
Sbjct: 1   MAIAFKSGVFF-LQSPKSQIGFRHSSPPDSSLSFKRFTPMASLSTSSPTLGLADTFTQLK 60

Query: 61  EQGKVALIPYITAGDPDLSTTAEALKVLSTCGSDIIELGVPYSDPLADGPVIQAAATRSL 120
           +QGKVA IPYITAGDPDLSTTAEALKVL  CGSDIIELGVPYSDPLADGPVIQAAATRSL
Sbjct: 61  KQGKVAFIPYITAGDPDLSTTAEALKVLDACGSDIIELGVPYSDPLADGPVIQAAATRSL 120

Query: 121 ARETNFNAIISMLKGVIPELSCPISLFTYYNPILKRGIENFMMIIKDAGVRGLVVPDVPL 180
            R TN ++I+ ML  V+P++SCPISLFTYYNPILKRG+  FM  I+  GV+GLVVPDVPL
Sbjct: 121 ERGTNLDSILEMLDKVVPQISCPISLFTYYNPILKRGLGKFMSSIRAVGVQGLVVPDVPL 180

Query: 181 EETEILRKEAVKYNIELVLLTTPTTPKDRMKNIAEASEGFVYLVSSVGVTGTRTSVSGRV 240
           EETE+LRKEA+  +IELVLLTTPTTP +RMK I +ASEGF+YLVSS+GVTG R+SVSG+V
Sbjct: 181 EETEMLRKEALNNDIELVLLTTPTTPTERMKLIVDASEGFIYLVSSIGVTGARSSVSGKV 240

Query: 241 QTLLEEVKEVTKKPVAVGFGISKPEHVKQVADWGADGIIIGSAMVKLLGEAQSPEEGLKE 300
           Q+LL+++KE T KPVAVGFGISKPEHVKQ+A WGADG+I+GSAMVKLLG+A+SP EGLKE
Sbjct: 241 QSLLKDIKEATDKPVAVGFGISKPEHVKQIAGWGADGVIVGSAMVKLLGDAKSPTEGLKE 300

Query: 301 LENFTISLKSAL 312
           LE  T SLKSAL
Sbjct: 301 LEKLTKSLKSAL 311

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038906368.11.0e-15795.19tryptophan synthase alpha chain-like [Benincasa hispida][more]
XP_008465320.11.5e-15693.27PREDICTED: tryptophan synthase alpha chain-like isoform X1 [Cucumis melo] >KAA00... [more]
XP_004141207.18.0e-15592.95tryptophan synthase alpha chain [Cucumis sativus] >KGN59758.1 hypothetical prote... [more]
XP_022932801.13.3e-15390.71tryptophan synthase alpha chain-like [Cucurbita moschata][more]
XP_023528646.14.4e-15390.71tryptophan synthase alpha chain-like [Cucurbita pepo subsp. pepo] >XP_023528647.... [more]
Match NameE-valueIdentityDescription
O227652.4e-11779.32Tryptophan synthase alpha chain OS=Arabidopsis thaliana OX=3702 GN=TRPA1 PE=1 SV... [more]
Q425295.2e-11770.83Tryptophan synthase alpha chain, chloroplastic OS=Arabidopsis thaliana OX=3702 G... [more]
P423901.1e-8555.41Indole-3-glycerol phosphate lyase, chloroplastic OS=Zea mays OX=4577 GN=BX1 PE=1... [more]
B8HPH28.7e-8056.44Tryptophan synthase alpha chain OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141)... [more]
B0C6F81.9e-7956.70Tryptophan synthase alpha chain OS=Acaryochloris marina (strain MBIC 11017) OX=3... [more]
Match NameE-valueIdentityDescription
A0A5D3E2H97.0e-15793.27Tryptophan synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold734G... [more]
A0A1S3CNL87.0e-15793.27Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103502969 PE=3 SV=1[more]
A0A0A0LD813.9e-15592.95Tryptophan synthase OS=Cucumis sativus OX=3659 GN=Csa_3G843770 PE=3 SV=1[more]
A0A6J1EXE21.6e-15390.71Tryptophan synthase OS=Cucurbita moschata OX=3662 GN=LOC111439260 PE=3 SV=1[more]
A0A1S4E5602.1e-15392.31Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103502969 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G02610.11.7e-11879.32Aldolase-type TIM barrel family protein [more]
AT3G54640.13.7e-11870.83tryptophan synthase alpha chain [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 50..312
e-value: 3.2E-109
score: 365.9
IPR002028Tryptophan synthase, alpha chainPFAMPF00290Trp_syntAcoord: 55..310
e-value: 1.0E-92
score: 309.7
IPR002028Tryptophan synthase, alpha chainTIGRFAMTIGR00262TIGR00262coord: 55..308
e-value: 3.5E-92
score: 305.9
IPR002028Tryptophan synthase, alpha chainPANTHERPTHR43406TRYPTOPHAN SYNTHASE, ALPHA CHAINcoord: 46..311
IPR002028Tryptophan synthase, alpha chainHAMAPMF_00131Trp_synth_alphacoord: 61..310
score: 35.980568
IPR002028Tryptophan synthase, alpha chainCDDcd04724Tryptophan_synthase_alphacoord: 65..308
e-value: 7.05801E-114
score: 327.126
NoneNo IPR availablePANTHERPTHR43406:SF6TRYPTOPHAN SYNTHASE ALPHA CHAINcoord: 46..311
IPR018204Tryptophan synthase, alpha chain, active sitePROSITEPS00167TRP_SYNTHASE_ALPHAcoord: 95..108
IPR011060Ribulose-phosphate binding barrelSUPERFAMILY51366Ribulose-phoshate binding barrelcoord: 51..292

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc10G12560.1Clc10G12560.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000162 tryptophan biosynthetic process
biological_process GO:0006568 tryptophan metabolic process
cellular_component GO:0009507 chloroplast
cellular_component GO:0005829 cytosol
cellular_component GO:0005634 nucleus
molecular_function GO:0004834 tryptophan synthase activity
molecular_function GO:0003824 catalytic activity