Clc10G12180 (gene) Watermelon (cordophanus) v2

Overview
NameClc10G12180
Typegene
OrganismCitrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
DescriptionMetal transporter Nramp6
LocationClcChr10: 25683091 .. 25688888 (+)
RNA-Seq ExpressionClc10G12180
SyntenyClc10G12180
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTGCTGCGAAGGGAGCTGAACAGGAAAAGGGAGGAGAGAGAAATGAAGGGAAGGAGGTTATTTAAGGGAGAGAGAAGTCTGAAAGAGTAAGCAAAGCGGGAGCTCTGACCACCAGCTTATCGAATCCACCATCGTCAATCCCCTTATCATATTCTGGATTAAGGTTCGTTCTTCAAATAACCCATTTCCTCAATTTCATTTTCATTCCGGATCATTTGCCTGAATTCTCTGTTTTGATTTTTGGTAAATATCGCAGAAAATTTAGCGAACTGAGATCTATTGCCCCTTTTCCTCTCTCTTTTTAACCAAAATGCCTTCATTTCTAATTCTTTGAAAAGATTGTGTCTATCCGATTCTCTTTCGAGTTTTTCTTTTTTCTTACCTATTCAGCTATGGAATAACGGAACAGGGGATTAGTTTTTAGTTCTGGATATTGTATAATTCTTCCTTCTTTTTCTGAGTCCACTTTTACTCTATTTTCGGTTAATTTCTCGTTTTACTTGTTTTCTGATGGAGATAGCAGATGGGTTGTTTGTTTGTTTGTTTTCTCTGAGCATTCTTTCTTGCTAACTTTTCAGATTTCATTTCGTTTCAAAAAGAGAGGGGAAAAAGGAGAGGAACAATAAAATGGCTGCTGGAGGGTCTGGTTCTGGGCAGCCACAATTTATTTTGAGGGCTGGAGATGAGAGTTTTTCGCACGCGCCGTTGATCGAGAACCCGGAAACTGATCAGATTGTTGTTCCTGATGTGAGCCTTTTAGCTTTGATGTTCTGATCATTAATACTTGAGAATTTTCAAATGATTGGATAATTAGTCATTTAATTGTACTTTGAATGAGACGATGTTAAAGTTTAAGCAACTTGTGTATGCTATAGAGTATTGGGTATGGACAAAAGTGCTGAAGGAATTTTTTGATTGAGAGCTACTGTTGCGTATTATGTGCAGAAGAAAAGCTGGAAGAACCTTTTTGCATACATGGGCCCGGGGTTTCTTGTTTCTATTGCATATATTGATCCTGGGAATTGTAAGATTTCTAGTTTCTCTATTTTGTGCACTACTTTTTGATTTGTGGTTTGTCTCTTTTATTCCATTAAGAATGGGATTCTGAAGGTTTTGTTTGGTTATTCTCAGTTGAGACTGATCTACAATCTGGAGCACAGTACAAGTATGGGGTACGTAAGCGGTCTTTTCGATATAGTTATTTTACTTTTAACAACCAACAAAGACATGATTTTGTGGTCAAAGACTAGAGTTTGTTATCATCTTTCAATATCTTATCCCCACACCCTTCTATGTGGATGGATTATTTTAATAAGTAATAAATTAAGTATGCTGCTATGAAGAGCTGAGATGATCCATTCACCATAGGCCTCTTCATTTAGATCATTTCTGTCCAACTATAAAAGATATATCACGCTATTGATTATATAAACATGTGTATGGTGCTGCCTCCAGCCTAATCCCATAAATCTACTCAAGACAAGTTTCAGAACTAGAAATTTTCTACAACTTTTCAATTATGTCATGTACTGAACATTCTGAATAGACTCAAATTTTATTTGCATTTATCTTTTCTTGTCGAAGCTAGTAAGTCCTGTCCTGTCATTAGAATTGCCCTTGCTTGTTATATGGACCTGACAACTAATATTGCTTCTCTCCCTACAGTTGCTTTGGATCATATTAGTGGCTTCCTTTGCTGCCCTCATCATTCAGTCCTTAGCAGCCAATCTAGGGGTCGTCACAGGTAAATGATTTCTGCATAACTTTTTTGTTTTCTTATCAAAATTGTGATATTTTCTTAGTTTCAACAGTTGTTTGACATCTTTCTTCTTCTTGTTTTTATGGAAAGTGTAGGAAAACATTTAGCAGAGCACTGTAAAGCTGAGTACCCTAAGGCGCAAAATTTCGTCCTCTGGATCCTTGCTGAAATTGCAATTGTTGCATGTGATATTCCCGAAGGTAAAGTGAAGTAATCCTATCCATTAAAAACCGTAATCGTTGAGTGCAAAGTACTCTTGGTCGTAGAAGAAATAAGCCCAATTATTGCATGTTTAATCTTTTGCATTTGGTGTACTCATGTTGGATGGTTTATTTAAAATGAATTTTCCGAGATTTGTACAATAGCAGTTACCCAATTATAAAGTTTATGAGTGGGAGATCTATATCAGTTGCCAATAACTATATCGAGGATAAGGGGAGCCAGAGGATAAGGGGAATATCATAACTACTCAGCCTATGCTGTGTAAAAGCTGATTAGATGTGCATTGACCAACTGTAGTGTTTGACATTTATATGATCAAGTGTACAAGCCAAACCGAAAAACATTTTCAAGTTAAGTTAAATTACAAATTTAGTCACCAAAGATGTTCAAGGACCAATTGGACAAAAAGTTGAACGTATAGGAACCTATTATACTCAAAATTGAAAGTTTAGGAACCTATTAGACACTTTTGAAAATTTAGGAACCAAATAAATAATAACTTCAAAGTTTAGAGAATAAATGTGTAATTTAACATTAAAAATACATAAACACAACATCATTGCTTTCATAAGGAGAAGAAAAATATCATTATTATTACTTCTCTCTGTAATAAATAAGCATTCAGATTTTCTATGAGGTATTGTATTCTGTATTTGCTACATGACTTTCATTGTCTTAGAATTCTTGTTTGTTGTTGAAGTTATTCTTATATCTCCTCCTTGATGTTTCCAGTTATAGGAACTGCTTTTGCATTGAATATGCTCTTCAGCATTCCTGTTTGGTGTGGCGTTCTTCTGACAGGGCTTAGTACACTATTACTTCTTGCACTGCAGCAATATGGGGTATTTCAAGATTTACTTTTTTCTGGATTTTACTTATCTTGTAGTAATAGAGGTTCCAAGCCTTGCACCAGTACTTAGGTTGGGAAATAATTCTCCCGTTTGTGCTAGTATAGTCAAGGGGATAGCATAATTTTAGAGCTGAACTTTAGCTTCTTTAAATGCTCACCATGACTGAGTTTCTATCCACAATGCAGATTAGAAAGCTTGAATTCTTGATTGCTTTTCTCGTATTGACAATTGCGATTTGCTTCTTCCTGGAGCTGGGCTATGCAAAGCCTGACGTTGGAGAAATTTTTTATGGGCTATTTGTCCCTCAATTGAAAGGAAGTGGTGCTACTGGTCTTGCAATTTCACTTCTTGGTGCTATGGTTATGCCGTATGTCATTTATATCCTTTGATAACATTCATAGCCTTCATTCCTTATACTTCTAATATTCTAAATCTAACTACCTGCTGCAGACACAATCTGTTTCTTCATTCAGCACTGGTGCTCTCTAGGAAAATACCCCGATCCCTTTCTGGCATCAAGGTGATGTTTCGTCTTCGTCAGTCTTAACCTTTATTCTCCTTATTACTTAAAAACTTCCGGGGTGAATTATAAACTATTTAATAGTTTACCTAATATGTAAGTAGGTTCATACAAGCCTGCTTCAAAGCTTTCATTGCTGCATAGTGCATTATTAATTAAATTGCAATTCTACATTCCATCGTACCAACGGTTTATCTTTTCTTTACAGGAAGCTTGCAGATTTTACATGATAGAGAGTGGCTTTGCTCTTATGGTGGCCTTCCTCATTAATGTGTCAGTCATTTCAGTTAGTGGTGCAGTTTGCAATGCCCCAGATCTAAATAAGGAGGACCAAATGAGCTGCAATGATTTGGACTTGAATAAAGCTTCATTTTTATTGAGAGTATGTGTGATCTAGAAACTTTGCCTGTATCCTTGTTTATTACCTCATGGGATTTTGTTCCTCATCGATGGTGTGCCAGTTTTATTCAGAAATCTTTTGTACTTTTTTCAGAATGTATTGGGTAAATGGAGTTCAAAACTTTTTGCCATTGCTTTGTTGGCATCTGGTCAGAGTTCTACCATAACAGGAACCTATGCAGGACAGTATGTTATGCAGGTATTATGCTGGTCCTTTTTCCATGTTAAAGCTCTGCTTTTCAATTTCTGAATTCTGGCTCTCCTCCATACATATCATTGGTATTGAGTAAATTGGAAATAACATGTTAGAGGTTTCAAAATTTTCTGAATGTTCATGGTCGTAAACTGGGCTACTTGTGAGTACTCCATAATGCCTGATTTCAAGTAACTTTTTGATACCTGTGATTTTTTTGATTGTTTCCATCTTTTGACAATTTCCTGTTTGGATTGCCTCTCTTTTGTAGGGGTTTCTCGATTTGAAACTGACGCCGTGGATCAGAAACTTTTTAACTCGAAGCTTAGCAATTGTTCCTAGTTTGATTGTCGCAATCATTGGTGGGTCTTCTGGGGCTGGGAAGCTGATCATTATTGCGTCGGTAAGAGATATACTTCCCACAAATGTTATTGAAGTCTAATTTCCATTCAGCTATGTATTGTCAATACCTCGTCTTTTTTCTAAGCATGTTATTAACCAAATTCAATTAATGTAGAAGTTGATAGAAAATAGTAGTAGAATTCACTTTGAAACATGCATTGTCGTTTATTGAAGCGGTAAACCTTAACCAATCAACAACCATAGAATGGATTTCTTATTCCATATGCTTCAGATTTAACGCTGATCCACCATCTTTTTCACTGGGGGCCCAAGTTTCTTGGCACAGTTCAGTTGGAGTGTGCTGACTAAATACCTCTTAAGATTGAAGGCCAAAGCATTTTGACTCCCTGCAATCCTCTTTTTTGGCAGATGATTTTGTCATTTGAACTCCCTTTTGCTCTCGTTCCTCTTCTGAAGTTTACAAGCAGTAAGGCCAAGATGGGACCACATGTCAACTCTACTGCGGTAATTTCCGGCTGCATCTCTATGATACTCGACGTTTTGATTTTTGTTATCTTTTTTTAGATGATCCCAAACAAAGAGCTAAAGATCATTCAAATTCTTATAACGAACCCATATGATAGACATTTGGTTTTCGAAAATGTGTTTGTTTTTCACTTTTTTTTTTTTTTCATTTCATGACTTCTATCTTTTCTAATGAGACACTGCTATTTATTTACCAAAGTGGTTCTTATCTTGAATTTTCATGTAGGGTAGAATTTGAAGCCTTTTGTAATTTTTCCTAAACTATTATACCACAAACGAATGGCTATTTAATTTTAAAAATAAATACTAATCCTATTAACCATGGAAAGGTTAAGAATTCTCTTTGAAATGCATTCTCCTTTTAGTTCTTATCTTCAAGGATGAACTGATGAATGTCATGAAATGGGTCTCACACTCTTGACAACACCCAGCTGATCATCATAGTTATTTTCTGATCTTCGTTGTATATTTTTGAAATGCAGATTACAGTATTGACATGGATTATTGGCTTCCTCATCATGGCTATAAATATATACTACCTCATGAGCCGTTTCATCCACATGCTCCTTCATAACGATCTACACCTCGCAGCAGTTGTCTTCATAGGGATACTTGGATTTTCAGGCGTGGCACTATATTTAGCTGGAATTGCTTATCTGGTTTTGAGAAAGAGCAAGGAGATCACTCATCTCTTAGCACTAACAACAGATGAAAGTAGAAGACTGAGCAACGAGCCGAGTAAGACATCGGGATATAGTCTCCCGAACGAAGATATAGTAAGCATGCAGTTGCCTCAACGAGTTAGAACTGCCAATGATGTAAACTGACAGATTATAGTTAAAAACTTGAACCCCATAGAAGGAAGGCAGATCCTTGGGTGCTGGTTTCATTTGTTTGGAAATAGAACAAACAGATGAAACTCAGAAGCACAGCAAAATGGAAAAAAAGAAAAAAGAAAAAAGA

mRNA sequence

ATGCTGCTGCGAAGGGAGCTGAACAGGAAAAGGGAGGAGAGAGAAATGAAGGGAAGGAGAGTAAGCAAAGCGGGAGCTCTGACCACCAGCTTATCGAATCCACCATCGTCAATCCCCTTATCATATTCTGGATTAAGATTTCATTTCGTTTCAAAAAGAGAGGGGAAAAAGGAGAGGAACAATAAAATGGCTGCTGGAGGGTCTGGTTCTGGGCAGCCACAATTTATTTTGAGGGCTGGAGATGAGAGTTTTTCGCACGCGCCGTTGATCGAGAACCCGGAAACTGATCAGATTGTTGTTCCTGATAAGAAAAGCTGGAAGAACCTTTTTGCATACATGGGCCCGGGGTTTCTTGTTTCTATTGCATATATTGATCCTGGGAATTTTGAGACTGATCTACAATCTGGAGCACAGTACAAGTATGGGTTGCTTTGGATCATATTAGTGGCTTCCTTTGCTGCCCTCATCATTCAGTCCTTAGCAGCCAATCTAGGGGTCGTCACAGGAAAACATTTAGCAGAGCACTGTAAAGCTGAGTACCCTAAGGCGCAAAATTTCGTCCTCTGGATCCTTGCTGAAATTGCAATTGTTGCATGTGATATTCCCGAAGTTATAGGAACTGCTTTTGCATTGAATATGCTCTTCAGCATTCCTGTTTGGTGTGGCGTTCTTCTGACAGGGCTTAGTACACTATTACTTCTTGCACTGCAGCAATATGGGATTAGAAAGCTTGAATTCTTGATTGCTTTTCTCGTATTGACAATTGCGATTTGCTTCTTCCTGGAGCTGGGCTATGCAAAGCCTGACGTTGGAGAAATTTTTTATGGGCTATTTGTCCCTCAATTGAAAGGAAGTGGTGCTACTGGTCTTGCAATTTCACTTCTTGGTGCTATGGTTATGCCACACAATCTGTTTCTTCATTCAGCACTGGTGCTCTCTAGGAAAATACCCCGATCCCTTTCTGGCATCAAGGAAGCTTGCAGATTTTACATGATAGAGAGTGGCTTTGCTCTTATGGTGGCCTTCCTCATTAATGTGTCAGTCATTTCAGTTAGTGGTGCAGTTTGCAATGCCCCAGATCTAAATAAGGAGGACCAAATGAGCTGCAATGATTTGGACTTGAATAAAGCTTCATTTTTATTGAGAAATGTATTGGGTAAATGGAGTTCAAAACTTTTTGCCATTGCTTTGTTGGCATCTGGTCAGAGTTCTACCATAACAGGAACCTATGCAGGACAGTATGTTATGCAGGGGTTTCTCGATTTGAAACTGACGCCGTGGATCAGAAACTTTTTAACTCGAAGCTTAGCAATTGTTCCTAGTTTGATTGTCGCAATCATTGGTGGGTCTTCTGGGGCTGGGAAGCTGATCATTATTGCGTCGATTACAGTATTGACATGGATTATTGGCTTCCTCATCATGGCTATAAATATATACTACCTCATGAGCCGTTTCATCCACATGCTCCTTCATAACGATCTACACCTCGCAGCAGTTGTCTTCATAGGGATACTTGGATTTTCAGGCGTGGCACTATATTTAGCTGGAATTGCTTATCTGGTTTTGAGAAAGAGCAAGGAGATCACTCATCTCTTAGCACTAACAACAGATGAAAGTAGAAGACTGAGCAACGAGCCGAGTAAGACATCGGGATATAGTCTCCCGAACGAAGATATAGTAAGCATGCAGTTGCCTCAACGAGTTAGAACTGCCAATGATGTAAACTGACAGATTATAGTTAAAAACTTGAACCCCATAGAAGGAAGGCAGATCCTTGGGTGCTGGTTTCATTTGTTTGGAAATAGAACAAACAGATGAAACTCAGAAGCACAGCAAAATGGAAAAAAAGAAAAAAGAAAAAAGA

Coding sequence (CDS)

ATGCTGCTGCGAAGGGAGCTGAACAGGAAAAGGGAGGAGAGAGAAATGAAGGGAAGGAGAGTAAGCAAAGCGGGAGCTCTGACCACCAGCTTATCGAATCCACCATCGTCAATCCCCTTATCATATTCTGGATTAAGATTTCATTTCGTTTCAAAAAGAGAGGGGAAAAAGGAGAGGAACAATAAAATGGCTGCTGGAGGGTCTGGTTCTGGGCAGCCACAATTTATTTTGAGGGCTGGAGATGAGAGTTTTTCGCACGCGCCGTTGATCGAGAACCCGGAAACTGATCAGATTGTTGTTCCTGATAAGAAAAGCTGGAAGAACCTTTTTGCATACATGGGCCCGGGGTTTCTTGTTTCTATTGCATATATTGATCCTGGGAATTTTGAGACTGATCTACAATCTGGAGCACAGTACAAGTATGGGTTGCTTTGGATCATATTAGTGGCTTCCTTTGCTGCCCTCATCATTCAGTCCTTAGCAGCCAATCTAGGGGTCGTCACAGGAAAACATTTAGCAGAGCACTGTAAAGCTGAGTACCCTAAGGCGCAAAATTTCGTCCTCTGGATCCTTGCTGAAATTGCAATTGTTGCATGTGATATTCCCGAAGTTATAGGAACTGCTTTTGCATTGAATATGCTCTTCAGCATTCCTGTTTGGTGTGGCGTTCTTCTGACAGGGCTTAGTACACTATTACTTCTTGCACTGCAGCAATATGGGATTAGAAAGCTTGAATTCTTGATTGCTTTTCTCGTATTGACAATTGCGATTTGCTTCTTCCTGGAGCTGGGCTATGCAAAGCCTGACGTTGGAGAAATTTTTTATGGGCTATTTGTCCCTCAATTGAAAGGAAGTGGTGCTACTGGTCTTGCAATTTCACTTCTTGGTGCTATGGTTATGCCACACAATCTGTTTCTTCATTCAGCACTGGTGCTCTCTAGGAAAATACCCCGATCCCTTTCTGGCATCAAGGAAGCTTGCAGATTTTACATGATAGAGAGTGGCTTTGCTCTTATGGTGGCCTTCCTCATTAATGTGTCAGTCATTTCAGTTAGTGGTGCAGTTTGCAATGCCCCAGATCTAAATAAGGAGGACCAAATGAGCTGCAATGATTTGGACTTGAATAAAGCTTCATTTTTATTGAGAAATGTATTGGGTAAATGGAGTTCAAAACTTTTTGCCATTGCTTTGTTGGCATCTGGTCAGAGTTCTACCATAACAGGAACCTATGCAGGACAGTATGTTATGCAGGGGTTTCTCGATTTGAAACTGACGCCGTGGATCAGAAACTTTTTAACTCGAAGCTTAGCAATTGTTCCTAGTTTGATTGTCGCAATCATTGGTGGGTCTTCTGGGGCTGGGAAGCTGATCATTATTGCGTCGATTACAGTATTGACATGGATTATTGGCTTCCTCATCATGGCTATAAATATATACTACCTCATGAGCCGTTTCATCCACATGCTCCTTCATAACGATCTACACCTCGCAGCAGTTGTCTTCATAGGGATACTTGGATTTTCAGGCGTGGCACTATATTTAGCTGGAATTGCTTATCTGGTTTTGAGAAAGAGCAAGGAGATCACTCATCTCTTAGCACTAACAACAGATGAAAGTAGAAGACTGAGCAACGAGCCGAGTAAGACATCGGGATATAGTCTCCCGAACGAAGATATAGTAAGCATGCAGTTGCCTCAACGAGTTAGAACTGCCAATGATGTAAACTGA

Protein sequence

MLLRRELNRKREEREMKGRRVSKAGALTTSLSNPPSSIPLSYSGLRFHFVSKREGKKERNNKMAAGGSGSGQPQFILRAGDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPKAQNFVLWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIRKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPHNLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPDLNKEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLKLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASITVLTWIIGFLIMAINIYYLMSRFIHMLLHNDLHLAAVVFIGILGFSGVALYLAGIAYLVLRKSKEITHLLALTTDESRRLSNEPSKTSGYSLPNEDIVSMQLPQRVRTANDVN
Homology
BLAST of Clc10G12180 vs. NCBI nr
Match: TYK27020.1 (metal transporter Nramp6 [Cucumis melo var. makuwa])

HSP 1 Score: 956.8 bits (2472), Expect = 8.3e-275
Identity = 504/556 (90.65%), Postives = 520/556 (93.53%), Query Frame = 0

Query: 52  KREGKKERNNKMAAGGSGSGQPQFILRAGDESFSHAPLIENPETDQIVVPDKKSWKNLFA 111
           K+EGK +RNNKMAAGGSGSGQPQF++RAGD+SFSHAPLIENPETDQI+VPDK+SWKNLFA
Sbjct: 14  KKEGKNKRNNKMAAGGSGSGQPQFLVRAGDDSFSHAPLIENPETDQIIVPDKESWKNLFA 73

Query: 112 YMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKH 171
           YMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKH
Sbjct: 74  YMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKH 133

Query: 172 LAEHCKAEYPKAQNFVLWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTL 231
           LAEHCKAEYPKAQNF+LW+LAEIAIVACDIPEVIGTAFALNMLF IPVWCGVLLTGLSTL
Sbjct: 134 LAEHCKAEYPKAQNFILWVLAEIAIVACDIPEVIGTAFALNMLFGIPVWCGVLLTGLSTL 193

Query: 232 LLLALQQYGIRKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLA 291
           LLLALQQYGIRKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLA
Sbjct: 194 LLLALQQYGIRKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLA 253

Query: 292 ISLLGAMVMPHNLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISV 351
           ISLLGAMVMPHNLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISV
Sbjct: 254 ISLLGAMVMPHNLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISV 313

Query: 352 SGAVCNAPDLNKEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYA 411
           SGAVCNAPDLNKEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYA
Sbjct: 314 SGAVCNAPDLNKEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYA 373

Query: 412 GQYVMQGFLDLKLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIAS---------- 471
           GQYVMQGFLDLKLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIAS          
Sbjct: 374 GQYVMQGFLDLKLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFA 433

Query: 472 ----------------------ITVLTWIIGFLIMAINIYYLMSRFIHMLLHNDLHLAAV 531
                                 ITVLTWIIGFLIMAINIYYLMSRFIH+LLHN+LHLAAV
Sbjct: 434 LVPLLKFTSSKAKMGPHVNSTAITVLTWIIGFLIMAINIYYLMSRFIHVLLHNNLHLAAV 493

Query: 532 VFIGILGFSGVALYLAGIAYLVLRKSKEITHLLALTTDESRRLSNEPSKTSGYSLPNEDI 576
           VFIGILGFSGVALYLAGIAYLVLRK+KEI+HLLALTT+ESRRLSN+PSKTSGY LPNEDI
Sbjct: 494 VFIGILGFSGVALYLAGIAYLVLRKTKEISHLLALTTEESRRLSNDPSKTSGYDLPNEDI 553

BLAST of Clc10G12180 vs. NCBI nr
Match: KAA0037704.1 (metal transporter Nramp6 [Cucumis melo var. makuwa])

HSP 1 Score: 940.3 bits (2429), Expect = 8.0e-270
Identity = 496/545 (91.01%), Postives = 510/545 (93.58%), Query Frame = 0

Query: 63  MAAGGSGSGQPQFILRAGDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA 122
           MAAGGSGSGQPQF++RAGD+SFSHAPLIENPETDQI+VPDK+SWKNLFAYMGPGFLVSIA
Sbjct: 1   MAAGGSGSGQPQFLVRAGDDSFSHAPLIENPETDQIIVPDKESWKNLFAYMGPGFLVSIA 60

Query: 123 YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK 182
           YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK
Sbjct: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK 120

Query: 183 AQNFVLWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR 242
           AQNF+LW+LAEIAIVACDIPEVIGTAFALNMLF IPVWCGVLLTGLSTLLLLALQQYGIR
Sbjct: 121 AQNFILWVLAEIAIVACDIPEVIGTAFALNMLFGIPVWCGVLLTGLSTLLLLALQQYGIR 180

Query: 243 KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 302
           KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH
Sbjct: 181 KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 240

Query: 303 NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPDLN 362
           NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPDLN
Sbjct: 241 NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPDLN 300

Query: 363 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 422
           KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL
Sbjct: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360

Query: 423 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIAS--------------------- 482
           KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIAS                     
Sbjct: 361 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK 420

Query: 483 -----------ITVLTWIIGFLIMAINIYYLMSRFIHMLLHNDLHLAAVVFIGILGFSGV 542
                      ITVLTWIIGFLIMAINIYYLMSRFIH+LLHN+LHLAAVVFIGILGFSGV
Sbjct: 421 AKMGPHVNSTAITVLTWIIGFLIMAINIYYLMSRFIHVLLHNNLHLAAVVFIGILGFSGV 480

Query: 543 ALYLAGIAYLVLRKSKEITHLLALTTDESRRLSNEPSKTSGYSLPNEDIVSMQLPQRVRT 576
           ALYLAGIAYLVLRK+KEI+HLLALTT+ESRRLSN+PSKTSGY LPNEDIVSMQLPQR+RT
Sbjct: 481 ALYLAGIAYLVLRKTKEISHLLALTTEESRRLSNDPSKTSGYDLPNEDIVSMQLPQRIRT 540

BLAST of Clc10G12180 vs. NCBI nr
Match: XP_038905496.1 (metal transporter Nramp6-like [Benincasa hispida] >XP_038905497.1 metal transporter Nramp6-like [Benincasa hispida])

HSP 1 Score: 937.9 bits (2423), Expect = 4.0e-269
Identity = 495/545 (90.83%), Postives = 508/545 (93.21%), Query Frame = 0

Query: 63  MAAGGSGSGQPQFILRAGDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA 122
           MAAGGSGSGQPQF++RAGDESFSHAPLIENPETDQI+VPDKKSWKNLFAYMGPGFLVSIA
Sbjct: 1   MAAGGSGSGQPQFLVRAGDESFSHAPLIENPETDQIIVPDKKSWKNLFAYMGPGFLVSIA 60

Query: 123 YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK 182
           YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK
Sbjct: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK 120

Query: 183 AQNFVLWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR 242
           AQNF+LW+LAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR
Sbjct: 121 AQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR 180

Query: 243 KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 302
           KLEFLIAFLVLTIAICFFLELGYAKPDVGEI YGLFVPQLKGSGATGLAISLLGAMVMPH
Sbjct: 181 KLEFLIAFLVLTIAICFFLELGYAKPDVGEILYGLFVPQLKGSGATGLAISLLGAMVMPH 240

Query: 303 NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPDLN 362
           NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCN+PDLN
Sbjct: 241 NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNSPDLN 300

Query: 363 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 422
           KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL
Sbjct: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360

Query: 423 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIAS--------------------- 482
           KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIAS                     
Sbjct: 361 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK 420

Query: 483 -----------ITVLTWIIGFLIMAINIYYLMSRFIHMLLHNDLHLAAVVFIGILGFSGV 542
                      ITVLTWIIGFLIMAINIYYLM+RFIH++LHNDL LA VVFIGILGFSG+
Sbjct: 421 TKMGPHVNSTAITVLTWIIGFLIMAINIYYLMTRFIHVILHNDLQLAVVVFIGILGFSGM 480

Query: 543 ALYLAGIAYLVLRKSKEITHLLALTTDESRRLSNEPSKTSGYSLPNEDIVSMQLPQRVRT 576
           ALYLAGIAYLVLRK+KEITHLLALTT+ESRRLSNEPSKTSGY LPNEDIVSMQLPQR+RT
Sbjct: 481 ALYLAGIAYLVLRKTKEITHLLALTTEESRRLSNEPSKTSGYGLPNEDIVSMQLPQRIRT 540

BLAST of Clc10G12180 vs. NCBI nr
Match: XP_011651819.2 (metal transporter Nramp6 [Cucumis sativus] >XP_031739044.1 metal transporter Nramp6 [Cucumis sativus] >KGN58926.2 hypothetical protein Csa_002390 [Cucumis sativus])

HSP 1 Score: 929.9 bits (2402), Expect = 1.1e-266
Identity = 493/545 (90.46%), Postives = 506/545 (92.84%), Query Frame = 0

Query: 63  MAAGGSGSGQPQFILRAGDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA 122
           MAAGGSGSGQPQF++RAGDESFSHAPLIENPETDQI+VPDKKSWKNLFAYMGPGFLVSIA
Sbjct: 1   MAAGGSGSGQPQFLVRAGDESFSHAPLIENPETDQIIVPDKKSWKNLFAYMGPGFLVSIA 60

Query: 123 YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK 182
           YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK
Sbjct: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK 120

Query: 183 AQNFVLWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR 242
           AQNF+LW+LAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR
Sbjct: 121 AQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR 180

Query: 243 KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 302
           KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH
Sbjct: 181 KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 240

Query: 303 NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPDLN 362
           NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCN+PDLN
Sbjct: 241 NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNSPDLN 300

Query: 363 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 422
           KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL
Sbjct: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360

Query: 423 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIAS--------------------- 482
           KLTPW+RNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIAS                     
Sbjct: 361 KLTPWVRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK 420

Query: 483 -----------ITVLTWIIGFLIMAINIYYLMSRFIHMLLHNDLHLAAVVFIGILGFSGV 542
                      ITVLTWIIGFLIMAINIYYLMSRFIH+LLHNDLHLA V+ IGILGFSGV
Sbjct: 421 AKMGPHVNSTAITVLTWIIGFLIMAINIYYLMSRFIHVLLHNDLHLAVVILIGILGFSGV 480

Query: 543 ALYLAGIAYLVLRKSKEITHLLALTTDESRRLSNEPSKTSGYSLPNEDIVSMQLPQRVRT 576
           ALYLAGIAYLVLRK+KEI+HLLALT +ESRRLSNE SKTSGY LPNED VSMQLPQR+RT
Sbjct: 481 ALYLAGIAYLVLRKTKEISHLLALTAEESRRLSNELSKTSGYDLPNED-VSMQLPQRIRT 540

BLAST of Clc10G12180 vs. NCBI nr
Match: KAA0048292.1 (metal transporter Nramp6 [Cucumis melo var. makuwa] >TYK14176.1 metal transporter Nramp6 [Cucumis melo var. makuwa])

HSP 1 Score: 915.6 bits (2365), Expect = 2.1e-262
Identity = 484/545 (88.81%), Postives = 501/545 (91.93%), Query Frame = 0

Query: 63  MAAGGSGSGQPQFILRAGDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA 122
           MAAGGSGSGQPQF++RAGDE+FSHAPLIENPETDQI+VPDK+SWKNLFAYMGPGFLVSIA
Sbjct: 1   MAAGGSGSGQPQFLVRAGDENFSHAPLIENPETDQIIVPDKESWKNLFAYMGPGFLVSIA 60

Query: 123 YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK 182
           YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK
Sbjct: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK 120

Query: 183 AQNFVLWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR 242
           AQNF+LW+LAEIAIVACDIPEVIGTAFALNMLF IPVWCGVLLTGLSTLLLLALQQYGIR
Sbjct: 121 AQNFILWVLAEIAIVACDIPEVIGTAFALNMLFRIPVWCGVLLTGLSTLLLLALQQYGIR 180

Query: 243 KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 302
           KLEFLIAFLVLTIA+CFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH
Sbjct: 181 KLEFLIAFLVLTIAVCFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 240

Query: 303 NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPDLN 362
           NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCN+PDLN
Sbjct: 241 NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNSPDLN 300

Query: 363 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 422
           KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL
Sbjct: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360

Query: 423 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIAS--------------------- 482
           KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIAS                     
Sbjct: 361 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK 420

Query: 483 -----------ITVLTWIIGFLIMAINIYYLMSRFIHMLLHNDLHLAAVVFIGILGFSGV 542
                      ITVLTWIIG LIMAINIYYLMSRFIH+LLHNDLHLAAVV IGILGFSGV
Sbjct: 421 AKMGPHVNSTAITVLTWIIGLLIMAINIYYLMSRFIHVLLHNDLHLAAVVSIGILGFSGV 480

Query: 543 ALYLAGIAYLVLRKSKEITHLLALTTDESRRLSNEPSKTSGYSLPNEDIVSMQLPQRVRT 576
           ALYL+GIAYLV RK+KEI+HLLALTT ++RRLSNEPS  S Y  PNED VSMQLPQR+R+
Sbjct: 481 ALYLSGIAYLVFRKTKEISHLLALTTVDNRRLSNEPSNRSRYDPPNEDTVSMQLPQRIRS 540

BLAST of Clc10G12180 vs. ExPASy Swiss-Prot
Match: Q9S9N8 (Metal transporter Nramp6 OS=Arabidopsis thaliana OX=3702 GN=NRAMP6 PE=2 SV=2)

HSP 1 Score: 720.7 bits (1859), Expect = 1.3e-206
Identity = 383/529 (72.40%), Postives = 438/529 (82.80%), Query Frame = 0

Query: 81  DESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIAYIDPGNFETDLQSGAQYK 140
           + S S++PLIEN +++QI+VP+KKSWKN F+Y+GPGFLVSIAYIDPGNFETDLQSGAQYK
Sbjct: 10  NRSISNSPLIENSDSNQILVPEKKSWKNFFSYLGPGFLVSIAYIDPGNFETDLQSGAQYK 69

Query: 141 YGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPKAQNFVLWILAEIAIVACD 200
           Y LLWIILVAS AAL+IQSLAANLGVVTGKHLAEHC+AEY K  NF+LW++AEIA+VACD
Sbjct: 70  YELLWIILVASCAALVIQSLAANLGVVTGKHLAEHCRAEYSKVPNFMLWVVAEIAVVACD 129

Query: 201 IPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIRKLEFLIAFLVLTIAICFF 260
           IPEVIGTAFALNMLF+IPVW GVLLTGLSTL+LLALQQYGIRKLEFLIAFLV TIA+CFF
Sbjct: 130 IPEVIGTAFALNMLFNIPVWIGVLLTGLSTLILLALQQYGIRKLEFLIAFLVFTIALCFF 189

Query: 261 LELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPHNLFLHSALVLSRKIPRSL 320
           +EL Y+KPD  E+ YGLFVPQLKG+GATGLAISLLGAMVMPHNLFLHSALVLSRKIPRS+
Sbjct: 190 VELHYSKPDPKEVLYGLFVPQLKGNGATGLAISLLGAMVMPHNLFLHSALVLSRKIPRSV 249

Query: 321 SGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPDLNKEDQMSCNDLDLNKASFL 380
           +GIKEACR+Y+IESG ALMVAFLINVSVISVSGAVCNA DL+ ED+ SC DLDLNKASFL
Sbjct: 250 TGIKEACRYYLIESGLALMVAFLINVSVISVSGAVCNASDLSPEDRASCQDLDLNKASFL 309

Query: 381 LRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLKLTPWIRNFLTRSLAIVP 440
           LRNV+GKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL+L PW+RNFLTR LAI+P
Sbjct: 310 LRNVVGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLRLEPWLRNFLTRCLAIIP 369

Query: 441 SLIVAIIGGSSGAGKLIIIAS--------------------------------ITVLTWI 500
           SLIVA+IGGS+GAGKLIIIAS                                I+ +TWI
Sbjct: 370 SLIVALIGGSAGAGKLIIIASMILSFELPFALVPLLKFTSSKTKMGSHANSLVISSVTWI 429

Query: 501 IGFLIMAINIYYLMSRFIHMLLHNDLHLAAVVFIGILGFSGVALYLAGIAYLVLRKSKE- 560
           IG LIM INIYYL+S FI +LLH+ ++L A+VF+G+LGFSG+A YLA I+YLVLRK++E 
Sbjct: 430 IGGLIMGINIYYLVSSFIKLLLHSHMNLVAIVFLGVLGFSGIATYLAAISYLVLRKNRES 489

Query: 561 -ITHLLALTTDESRRLSNEPSKTSGYSLPNEDIVSMQLPQRVRTANDVN 576
             TH L  +  ++             +LP EDI +MQLP RV    D+N
Sbjct: 490 SSTHFLDFSNSQTEE-----------TLPREDIANMQLPNRVAVIGDLN 527

BLAST of Clc10G12180 vs. ExPASy Swiss-Prot
Match: Q9SAH8 (Metal transporter Nramp1 OS=Arabidopsis thaliana OX=3702 GN=NRAMP1 PE=1 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 2.2e-206
Identity = 388/538 (72.12%), Postives = 443/538 (82.34%), Query Frame = 0

Query: 68  SGSGQPQFILRA-GDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIAYIDP 127
           +GSG+ QFI  + G+ SFS++PLIEN +++QI+V +KKSWKN FAY+GPGFLVSIAYIDP
Sbjct: 4   TGSGRSQFISSSGGNRSFSNSPLIENSDSNQIIVSEKKSWKNFFAYLGPGFLVSIAYIDP 63

Query: 128 GNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPKAQNF 187
           GNFETDLQ+GA YKY LLWIILVAS AAL+IQSLAANLGVVTGKHLAE C+AEY K  NF
Sbjct: 64  GNFETDLQAGAHYKYELLWIILVASCAALVIQSLAANLGVVTGKHLAEQCRAEYSKVPNF 123

Query: 188 VLWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIRKLEF 247
           +LW++AEIA+VACDIPEVIGTAFALNMLFSIPVW GVLLTGLSTL+LLALQ+YG+RKLEF
Sbjct: 124 MLWVVAEIAVVACDIPEVIGTAFALNMLFSIPVWIGVLLTGLSTLILLALQKYGVRKLEF 183

Query: 248 LIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPHNLFL 307
           LIAFLV TIAICFF+EL Y+KPD GE+ +GLFVPQLKG+GATGLAISLLGAMVMPHNLFL
Sbjct: 184 LIAFLVFTIAICFFVELHYSKPDPGEVLHGLFVPQLKGNGATGLAISLLGAMVMPHNLFL 243

Query: 308 HSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPDLNKEDQ 367
           HSALVLSRKIPRS SGIKEACRFY+IESG ALMVAFLINVSVISVSGAVCNAP+L+ ED+
Sbjct: 244 HSALVLSRKIPRSASGIKEACRFYLIESGLALMVAFLINVSVISVSGAVCNAPNLSPEDR 303

Query: 368 MSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLKLTP 427
            +C DLDLNKASFLLRNV+GKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL+L P
Sbjct: 304 ANCEDLDLNKASFLLRNVVGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLRLEP 363

Query: 428 WIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIAS------------------------- 487
           W+RN LTR LAI+PSLIVA+IGGS+GAGKLIIIAS                         
Sbjct: 364 WLRNLLTRCLAIIPSLIVALIGGSAGAGKLIIIASMILSFELPFALVPLLKFTSCKTKMG 423

Query: 488 -------ITVLTWIIGFLIMAINIYYLMSRFIHMLLHNDLHLAAVVFIGILGFSGVALYL 547
                  IT LTW+IG LIM INIYYL+S FI +L+H+ + L  VVF GILGF+G+ALYL
Sbjct: 424 SHVNPMAITALTWVIGGLIMGINIYYLVSSFIKLLIHSHMKLILVVFCGILGFAGIALYL 483

Query: 548 AGIAYLVLRKSKEITHLLALTTDESRRLSNEPSKTSGYSLPNEDIVSMQLPQRVRTAN 573
           A IAYLV RK++  T LL         +S +       +LP +DIV+MQLP RV T++
Sbjct: 484 AAIAYLVFRKNRVATSLL---------ISRDSQNVE--TLPRQDIVNMQLPCRVSTSD 530

BLAST of Clc10G12180 vs. ExPASy Swiss-Prot
Match: Q653V6 (Metal transporter Nramp3 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP3 PE=2 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 4.0e-195
Identity = 369/545 (67.71%), Postives = 431/545 (79.08%), Query Frame = 0

Query: 70  SGQPQFIL---RAGDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIAYIDP 129
           S QPQFI    R    +    PLI++ + DQIV+P+K SWKNLF+Y+GPGFLVSIAYIDP
Sbjct: 8   SSQPQFISSVERNNQSNGPGTPLIDSIDVDQIVIPEKNSWKNLFSYIGPGFLVSIAYIDP 67

Query: 130 GNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPKAQNF 189
           GNFETDLQ+GAQYKY LLWIIL+AS AALIIQSLAA LGVVTGKHLAEHC+AEYPKA NF
Sbjct: 68  GNFETDLQAGAQYKYELLWIILIASCAALIIQSLAARLGVVTGKHLAEHCRAEYPKATNF 127

Query: 190 VLWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIRKLEF 249
           +LWILAE+A+VACDIPEVIGTAFALNMLF IPVWCGVL+TGLSTL+LL LQQYG+RKLEF
Sbjct: 128 ILWILAELAVVACDIPEVIGTAFALNMLFKIPVWCGVLITGLSTLMLLLLQQYGVRKLEF 187

Query: 250 LIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPHNLFL 309
           LIA LV  IA CF +ELGY+KP+  E+  GLFVP+LKG+GATGLAISLLGAMVMPHNLFL
Sbjct: 188 LIAILVSLIATCFLVELGYSKPNSSEVVRGLFVPELKGNGATGLAISLLGAMVMPHNLFL 247

Query: 310 HSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPDLNKEDQ 369
           HSALVLSRK+PRS+ GIKEACRFYMIES FAL +AFLIN+S+ISVSGAVC + +L+ EDQ
Sbjct: 248 HSALVLSRKVPRSVHGIKEACRFYMIESAFALTIAFLINISIISVSGAVCGSDNLSPEDQ 307

Query: 370 MSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLKLTP 429
           M+C+DLDLNKASFLL+NVLG WSSKLFA+ALLASGQSSTITGTYAGQYVMQGFLDL++TP
Sbjct: 308 MNCSDLDLNKASFLLKNVLGNWSSKLFAVALLASGQSSTITGTYAGQYVMQGFLDLRMTP 367

Query: 430 WIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIAS------------------------- 489
           WIRN LTRSLAI+PSLIV+IIGGSS AG+LIIIAS                         
Sbjct: 368 WIRNLLTRSLAILPSLIVSIIGGSSAAGQLIIIASMILSFELPFALVPLLKFTSSRTKMG 427

Query: 490 -------ITVLTWIIGFLIMAINIYYLMSRFIHMLLHNDLHLAAVVFIGILGFSGVALYL 549
                  I+V+TW IG  I+ IN Y+L++ F+ +LLHN L   + VF GI GF G+ +Y+
Sbjct: 428 QHTNSKAISVITWGIGSFIVVINTYFLITSFVKLLLHNGLSTVSQVFSGIFGFLGMLIYM 487

Query: 550 AGIAYLVLRKSKEITHLLALTTDESRRLSNEPSKTSGYS----LPNEDIVSMQLPQRVRT 576
           A I YLV RK+++ T L  L  D + R+    + T G      LP EDI SMQLPQ+ RT
Sbjct: 488 AAILYLVFRKNRKAT-LPLLEGDSTVRIVGRDTATEGEGSLGHLPREDISSMQLPQQ-RT 547

BLAST of Clc10G12180 vs. ExPASy Swiss-Prot
Match: Q8H4H5 (Metal transporter Nramp5 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP5 PE=2 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 7.8e-143
Identity = 273/521 (52.40%), Postives = 361/521 (69.29%), Query Frame = 0

Query: 83  SFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIAYIDPGNFETDLQSGAQYKYG 142
           S +H    +  + D  ++  + +WK   A++GPGF+VS+AY+DPGN ETDLQ+GA ++Y 
Sbjct: 18  SAAHDQDAKKLDADDQLLMKEPAWKRFLAHVGPGFMVSLAYLDPGNLETDLQAGANHRYE 77

Query: 143 LLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPKAQNFVLWILAEIAIVACDIP 202
           LLW+IL+    ALIIQSLAANLGVVTG+HLAE CK+EYPK     LW+LAE+A++A DIP
Sbjct: 78  LLWVILIGLIFALIIQSLAANLGVVTGRHLAEICKSEYPKFVKIFLWLLAELAVIAADIP 137

Query: 203 EVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIRKLEFLIAFLVLTIAICFFLE 262
           EVIGTAFA N+LF IPVW GVL+TG STLLLL LQ+YG+RKLEFLI+ LV  +A CFF E
Sbjct: 138 EVIGTAFAFNILFHIPVWVGVLITGTSTLLLLGLQKYGVRKLEFLISMLVFVMAACFFGE 197

Query: 263 LGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPHNLFLHSALVLSRKIPRSLSG 322
           L   KP   E+  GLF+P+L G GAT  AI+LLGA+VMPHNLFLHSALVLSRK P S+ G
Sbjct: 198 LSIVKPPAKEVMKGLFIPRLNGDGATADAIALLGALVMPHNLFLHSALVLSRKTPASVRG 257

Query: 323 IKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPDLNKEDQMSCNDLDLNKASFLLR 382
           IK+ CRF++ ESGFAL VA LIN++V+SVSG  C++ +L++ED   C +L L+ +SFLL+
Sbjct: 258 IKDGCRFFLYESGFALFVALLINIAVVSVSGTACSSANLSQEDADKCANLSLDTSSFLLK 317

Query: 383 NVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLKLTPWIRNFLTRSLAIVPSL 442
           NVLGK S+ ++ +ALLASGQSSTITGTYAGQY+MQGFLD+++  W+RN +TR++AI PSL
Sbjct: 318 NVLGKSSAIVYGVALLASGQSSTITGTYAGQYIMQGFLDIRMRKWLRNLMTRTIAIAPSL 377

Query: 443 IVAIIGGSSGAGKLIIIAS--------------------------------ITVLTWIIG 502
           IV+IIGGS GAG+LIIIAS                                I V +W +G
Sbjct: 378 IVSIIGGSRGAGRLIIIASMILSFELPFALIPLLKFSSSKSKMGPHKNSIYIIVFSWFLG 437

Query: 503 FLIMAINIYYLMSRFIHMLLHNDLHLAAVVFIGILGFSGVALYLAGIAYLVLRKSKEITH 562
            LI+ IN+Y+L + F+  L+HNDL   A V +G   F  + +Y+  + YL +RK   +T 
Sbjct: 438 LLIIGINMYFLSTSFVGWLIHNDLPKYANVLVGAAVFPFMLVYIVAVVYLTIRKDSVVTF 497

Query: 563 L----LALTTDESRRLSNEPSKTSGYSLP-NEDIVSMQLPQ 567
           +    LA   D  +  + + +      LP  +D+  + LP+
Sbjct: 498 VADSSLAAVVDAEKADAGDLAVDDDEPLPYRDDLADIPLPR 538

BLAST of Clc10G12180 vs. ExPASy Swiss-Prot
Match: Q0D7E4 (Metal transporter Nramp1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP1 PE=2 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 3.6e-140
Identity = 260/452 (57.52%), Postives = 332/452 (73.45%), Query Frame = 0

Query: 105 SWKNLFAYMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANL 164
           +WK   +++GPGF+V +AY+DPGN ETDLQ+GA +KY LLW+IL+    ALIIQSL+ANL
Sbjct: 31  AWKRFLSHIGPGFMVCLAYLDPGNMETDLQAGANHKYELLWVILIGLIFALIIQSLSANL 90

Query: 165 GVVTGKHLAEHCKAEYPKAQNFVLWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVL 224
           GVVTG+HLAE CK EYP      LW+LAE+A++A DIPEVIGT FA N+LF IPVW GVL
Sbjct: 91  GVVTGRHLAELCKTEYPVWVKTCLWLLAELAVIASDIPEVIGTGFAFNLLFHIPVWTGVL 150

Query: 225 LTGLSTLLLLALQQYGIRKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKG 284
           + G STLLLL LQ+YG+RKLE ++A LV  +A CFF+E+   KP V E+  GLF+P+L G
Sbjct: 151 IAGSSTLLLLGLQRYGVRKLEVVVALLVFVMAGCFFVEMSIVKPPVNEVLQGLFIPRLSG 210

Query: 285 SGATGLAISLLGAMVMPHNLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLI 344
            GATG +I+LLGA+VMPHNLFLHSALVLSR  P S  G+K+ CRF++ ESG AL VA L+
Sbjct: 211 PGATGDSIALLGALVMPHNLFLHSALVLSRNTPASAKGMKDVCRFFLFESGIALFVALLV 270

Query: 345 NVSVISVSGAVCNAPDLNKEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSS 404
           N+++ISVSG VCNA +L+ ED + C+DL L+ +SFLLRNVLGK S+ ++ +ALLASGQSS
Sbjct: 271 NIAIISVSGTVCNATNLSPEDAVKCSDLTLDSSSFLLRNVLGKSSATVYGVALLASGQSS 330

Query: 405 TITGTYAGQYVMQGFLDLKLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIAS--- 464
           TITGTYAGQYVMQGFLD+K+  W+RN +TRS+AIVPSLIV+IIGGSSGAG+LI+IAS   
Sbjct: 331 TITGTYAGQYVMQGFLDIKMKQWLRNLMTRSIAIVPSLIVSIIGGSSGAGRLIVIASMIL 390

Query: 465 -----------------------------ITVLTWIIGFLIMAINIYYLMSRFIHMLLHN 524
                                        I   +W++GF+I+ INIY+L ++ +  +LHN
Sbjct: 391 SFELPFALIPLLKFSSSSNKMGENKNSIYIVGFSWVLGFVIIGINIYFLSTKLVGWILHN 450

BLAST of Clc10G12180 vs. ExPASy TrEMBL
Match: A0A5D3DUP0 (Metal transporter Nramp6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold901G00300 PE=3 SV=1)

HSP 1 Score: 956.8 bits (2472), Expect = 4.0e-275
Identity = 504/556 (90.65%), Postives = 520/556 (93.53%), Query Frame = 0

Query: 52  KREGKKERNNKMAAGGSGSGQPQFILRAGDESFSHAPLIENPETDQIVVPDKKSWKNLFA 111
           K+EGK +RNNKMAAGGSGSGQPQF++RAGD+SFSHAPLIENPETDQI+VPDK+SWKNLFA
Sbjct: 14  KKEGKNKRNNKMAAGGSGSGQPQFLVRAGDDSFSHAPLIENPETDQIIVPDKESWKNLFA 73

Query: 112 YMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKH 171
           YMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKH
Sbjct: 74  YMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKH 133

Query: 172 LAEHCKAEYPKAQNFVLWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTL 231
           LAEHCKAEYPKAQNF+LW+LAEIAIVACDIPEVIGTAFALNMLF IPVWCGVLLTGLSTL
Sbjct: 134 LAEHCKAEYPKAQNFILWVLAEIAIVACDIPEVIGTAFALNMLFGIPVWCGVLLTGLSTL 193

Query: 232 LLLALQQYGIRKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLA 291
           LLLALQQYGIRKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLA
Sbjct: 194 LLLALQQYGIRKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLA 253

Query: 292 ISLLGAMVMPHNLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISV 351
           ISLLGAMVMPHNLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISV
Sbjct: 254 ISLLGAMVMPHNLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISV 313

Query: 352 SGAVCNAPDLNKEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYA 411
           SGAVCNAPDLNKEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYA
Sbjct: 314 SGAVCNAPDLNKEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYA 373

Query: 412 GQYVMQGFLDLKLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIAS---------- 471
           GQYVMQGFLDLKLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIAS          
Sbjct: 374 GQYVMQGFLDLKLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFA 433

Query: 472 ----------------------ITVLTWIIGFLIMAINIYYLMSRFIHMLLHNDLHLAAV 531
                                 ITVLTWIIGFLIMAINIYYLMSRFIH+LLHN+LHLAAV
Sbjct: 434 LVPLLKFTSSKAKMGPHVNSTAITVLTWIIGFLIMAINIYYLMSRFIHVLLHNNLHLAAV 493

Query: 532 VFIGILGFSGVALYLAGIAYLVLRKSKEITHLLALTTDESRRLSNEPSKTSGYSLPNEDI 576
           VFIGILGFSGVALYLAGIAYLVLRK+KEI+HLLALTT+ESRRLSN+PSKTSGY LPNEDI
Sbjct: 494 VFIGILGFSGVALYLAGIAYLVLRKTKEISHLLALTTEESRRLSNDPSKTSGYDLPNEDI 553

BLAST of Clc10G12180 vs. ExPASy TrEMBL
Match: A0A5A7T2K9 (Metal transporter Nramp6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1593G00280 PE=3 SV=1)

HSP 1 Score: 940.3 bits (2429), Expect = 3.9e-270
Identity = 496/545 (91.01%), Postives = 510/545 (93.58%), Query Frame = 0

Query: 63  MAAGGSGSGQPQFILRAGDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA 122
           MAAGGSGSGQPQF++RAGD+SFSHAPLIENPETDQI+VPDK+SWKNLFAYMGPGFLVSIA
Sbjct: 1   MAAGGSGSGQPQFLVRAGDDSFSHAPLIENPETDQIIVPDKESWKNLFAYMGPGFLVSIA 60

Query: 123 YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK 182
           YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK
Sbjct: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK 120

Query: 183 AQNFVLWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR 242
           AQNF+LW+LAEIAIVACDIPEVIGTAFALNMLF IPVWCGVLLTGLSTLLLLALQQYGIR
Sbjct: 121 AQNFILWVLAEIAIVACDIPEVIGTAFALNMLFGIPVWCGVLLTGLSTLLLLALQQYGIR 180

Query: 243 KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 302
           KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH
Sbjct: 181 KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 240

Query: 303 NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPDLN 362
           NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPDLN
Sbjct: 241 NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPDLN 300

Query: 363 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 422
           KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL
Sbjct: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360

Query: 423 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIAS--------------------- 482
           KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIAS                     
Sbjct: 361 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK 420

Query: 483 -----------ITVLTWIIGFLIMAINIYYLMSRFIHMLLHNDLHLAAVVFIGILGFSGV 542
                      ITVLTWIIGFLIMAINIYYLMSRFIH+LLHN+LHLAAVVFIGILGFSGV
Sbjct: 421 AKMGPHVNSTAITVLTWIIGFLIMAINIYYLMSRFIHVLLHNNLHLAAVVFIGILGFSGV 480

Query: 543 ALYLAGIAYLVLRKSKEITHLLALTTDESRRLSNEPSKTSGYSLPNEDIVSMQLPQRVRT 576
           ALYLAGIAYLVLRK+KEI+HLLALTT+ESRRLSN+PSKTSGY LPNEDIVSMQLPQR+RT
Sbjct: 481 ALYLAGIAYLVLRKTKEISHLLALTTEESRRLSNDPSKTSGYDLPNEDIVSMQLPQRIRT 540

BLAST of Clc10G12180 vs. ExPASy TrEMBL
Match: A0A0A0LD68 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G714870 PE=3 SV=1)

HSP 1 Score: 932.9 bits (2410), Expect = 6.2e-268
Identity = 503/607 (82.87%), Postives = 528/607 (86.99%), Query Frame = 0

Query: 1   MLLRRELNRKREEREMKGRRVSKAGALTTSLSNPPSSIPLSYSGLRFHFVSKREGKKERN 60
           M++RRE+NRK+      G+                                ++EG+ +RN
Sbjct: 1   MVVRREVNRKKRGERNDGK--------------------------------EKEGRHKRN 60

Query: 61  NKMAAGGSGSGQPQFILRAGDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVS 120
           NKMAAGGSGSGQPQF++RAGDESFSHAPLIENPETDQI+VPDKKSWKNLFAYMGPGFLVS
Sbjct: 61  NKMAAGGSGSGQPQFLVRAGDESFSHAPLIENPETDQIIVPDKKSWKNLFAYMGPGFLVS 120

Query: 121 IAYIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEY 180
           IAYIDPGNFETDLQSGAQYKYGLLWIILVASF++LII SLAANLGVVTGKHLAEHCKAEY
Sbjct: 121 IAYIDPGNFETDLQSGAQYKYGLLWIILVASFSSLIIHSLAANLGVVTGKHLAEHCKAEY 180

Query: 181 PKAQNFVLWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYG 240
           PKAQNF+LW+LAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYG
Sbjct: 181 PKAQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYG 240

Query: 241 IRKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVM 300
           IRKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVM
Sbjct: 241 IRKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVM 300

Query: 301 PHNLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPD 360
           PHNLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVC++PD
Sbjct: 301 PHNLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCSSPD 360

Query: 361 LNKEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFL 420
           LNKEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFL
Sbjct: 361 LNKEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFL 420

Query: 421 DLKLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIAS------------------- 480
           DLKLTPW+RNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIAS                   
Sbjct: 421 DLKLTPWVRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTS 480

Query: 481 -------------ITVLTWIIGFLIMAINIYYLMSRFIHMLLHNDLHLAAVVFIGILGFS 540
                        ITVLTWIIGFLIMAINIYYLMSRFIH+LLHNDLHLA V+ IGILGFS
Sbjct: 481 SKAKMGPHVNSTAITVLTWIIGFLIMAINIYYLMSRFIHVLLHNDLHLAVVILIGILGFS 540

Query: 541 GVALYLAGIAYLVLRKSKEITHLLALTTDESRRLSNEPSKTSGYSLPNEDIVSMQLPQRV 576
           GVALYLAGIAYLVLRK+KEI+HLLALT +ESRRLSNE SKTSGY LPNED VSMQLPQR+
Sbjct: 541 GVALYLAGIAYLVLRKTKEISHLLALTAEESRRLSNELSKTSGYDLPNED-VSMQLPQRI 574

BLAST of Clc10G12180 vs. ExPASy TrEMBL
Match: A0A5A7TXN6 (Metal transporter Nramp6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold688G00430 PE=3 SV=1)

HSP 1 Score: 915.6 bits (2365), Expect = 1.0e-262
Identity = 484/545 (88.81%), Postives = 501/545 (91.93%), Query Frame = 0

Query: 63  MAAGGSGSGQPQFILRAGDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA 122
           MAAGGSGSGQPQF++RAGDE+FSHAPLIENPETDQI+VPDK+SWKNLFAYMGPGFLVSIA
Sbjct: 1   MAAGGSGSGQPQFLVRAGDENFSHAPLIENPETDQIIVPDKESWKNLFAYMGPGFLVSIA 60

Query: 123 YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK 182
           YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK
Sbjct: 61  YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK 120

Query: 183 AQNFVLWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR 242
           AQNF+LW+LAEIAIVACDIPEVIGTAFALNMLF IPVWCGVLLTGLSTLLLLALQQYGIR
Sbjct: 121 AQNFILWVLAEIAIVACDIPEVIGTAFALNMLFRIPVWCGVLLTGLSTLLLLALQQYGIR 180

Query: 243 KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 302
           KLEFLIAFLVLTIA+CFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH
Sbjct: 181 KLEFLIAFLVLTIAVCFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 240

Query: 303 NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPDLN 362
           NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCN+PDLN
Sbjct: 241 NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNSPDLN 300

Query: 363 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 422
           KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL
Sbjct: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360

Query: 423 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIAS--------------------- 482
           KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIAS                     
Sbjct: 361 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSK 420

Query: 483 -----------ITVLTWIIGFLIMAINIYYLMSRFIHMLLHNDLHLAAVVFIGILGFSGV 542
                      ITVLTWIIG LIMAINIYYLMSRFIH+LLHNDLHLAAVV IGILGFSGV
Sbjct: 421 AKMGPHVNSTAITVLTWIIGLLIMAINIYYLMSRFIHVLLHNDLHLAAVVSIGILGFSGV 480

Query: 543 ALYLAGIAYLVLRKSKEITHLLALTTDESRRLSNEPSKTSGYSLPNEDIVSMQLPQRVRT 576
           ALYL+GIAYLV RK+KEI+HLLALTT ++RRLSNEPS  S Y  PNED VSMQLPQR+R+
Sbjct: 481 ALYLSGIAYLVFRKTKEISHLLALTTVDNRRLSNEPSNRSRYDPPNEDTVSMQLPQRIRS 540

BLAST of Clc10G12180 vs. ExPASy TrEMBL
Match: A0A6J1F4P3 (metal transporter Nramp6-like OS=Cucurbita moschata OX=3662 GN=LOC111442317 PE=3 SV=1)

HSP 1 Score: 900.6 bits (2326), Expect = 3.4e-258
Identity = 476/543 (87.66%), Postives = 494/543 (90.98%), Query Frame = 0

Query: 63  MAAGGSGSGQPQFILRAGDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA 122
           MAAGGS SGQPQFI RAG+ESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA
Sbjct: 1   MAAGGSSSGQPQFIARAGNESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIA 60

Query: 123 YIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPK 182
           YIDPGNFETDLQSGAQYKY LLWIILVAS AALIIQSLAANLGVVTGKHLAEHCKAEYPK
Sbjct: 61  YIDPGNFETDLQSGAQYKYELLWIILVASCAALIIQSLAANLGVVTGKHLAEHCKAEYPK 120

Query: 183 AQNFVLWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIR 242
            QNF+LW+LAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTL LLALQQYGIR
Sbjct: 121 VQNFILWVLAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLFLLALQQYGIR 180

Query: 243 KLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPH 302
           KLEFLIAFLVLTIA+CFFLELGYAKPDVGE+FYGLFVPQLKGSGATGLAISLLGAMVMPH
Sbjct: 181 KLEFLIAFLVLTIAVCFFLELGYAKPDVGEVFYGLFVPQLKGSGATGLAISLLGAMVMPH 240

Query: 303 NLFLHSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPDLN 362
           NLFLHSALVLSRKIPRS+SGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCN+PDLN
Sbjct: 241 NLFLHSALVLSRKIPRSVSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNSPDLN 300

Query: 363 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 422
           KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL
Sbjct: 301 KEDQMSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL 360

Query: 423 KLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIAS--------------------- 482
           KLTPW+RNFLTR LAIVPSLIVAIIGGSSGAGKLIIIAS                     
Sbjct: 361 KLTPWVRNFLTRCLAIVPSLIVAIIGGSSGAGKLIIIASMILSFELPFALVPLLKFTSSR 420

Query: 483 -----------ITVLTWIIGFLIMAINIYYLMSRFIHMLLHNDLHLAAVVFIGILGFSGV 542
                      ITVLTWIIG LIMAINIYYLM+RFIH+LLHNDL L AVVFIGILGFSG+
Sbjct: 421 TKMGTHVNSTAITVLTWIIGCLIMAINIYYLMTRFIHVLLHNDLQLVAVVFIGILGFSGM 480

Query: 543 ALYLAGIAYLVLRKSKEITHLLALTTDESRRLSNEPSKTSGYSLPNEDIVSMQLPQRVRT 574
           A+YLAGIAYLV RK+KE THLLALTT+ES++LSN PSK SGY LPNED+ SMQLPQRVRT
Sbjct: 481 AIYLAGIAYLVFRKTKESTHLLALTTEESQQLSNTPSKRSGYGLPNEDLTSMQLPQRVRT 540

BLAST of Clc10G12180 vs. TAIR 10
Match: AT1G15960.1 (NRAMP metal ion transporter 6 )

HSP 1 Score: 720.7 bits (1859), Expect = 9.3e-208
Identity = 383/529 (72.40%), Postives = 438/529 (82.80%), Query Frame = 0

Query: 81  DESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIAYIDPGNFETDLQSGAQYK 140
           + S S++PLIEN +++QI+VP+KKSWKN F+Y+GPGFLVSIAYIDPGNFETDLQSGAQYK
Sbjct: 10  NRSISNSPLIENSDSNQILVPEKKSWKNFFSYLGPGFLVSIAYIDPGNFETDLQSGAQYK 69

Query: 141 YGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPKAQNFVLWILAEIAIVACD 200
           Y LLWIILVAS AAL+IQSLAANLGVVTGKHLAEHC+AEY K  NF+LW++AEIA+VACD
Sbjct: 70  YELLWIILVASCAALVIQSLAANLGVVTGKHLAEHCRAEYSKVPNFMLWVVAEIAVVACD 129

Query: 201 IPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIRKLEFLIAFLVLTIAICFF 260
           IPEVIGTAFALNMLF+IPVW GVLLTGLSTL+LLALQQYGIRKLEFLIAFLV TIA+CFF
Sbjct: 130 IPEVIGTAFALNMLFNIPVWIGVLLTGLSTLILLALQQYGIRKLEFLIAFLVFTIALCFF 189

Query: 261 LELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPHNLFLHSALVLSRKIPRSL 320
           +EL Y+KPD  E+ YGLFVPQLKG+GATGLAISLLGAMVMPHNLFLHSALVLSRKIPRS+
Sbjct: 190 VELHYSKPDPKEVLYGLFVPQLKGNGATGLAISLLGAMVMPHNLFLHSALVLSRKIPRSV 249

Query: 321 SGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPDLNKEDQMSCNDLDLNKASFL 380
           +GIKEACR+Y+IESG ALMVAFLINVSVISVSGAVCNA DL+ ED+ SC DLDLNKASFL
Sbjct: 250 TGIKEACRYYLIESGLALMVAFLINVSVISVSGAVCNASDLSPEDRASCQDLDLNKASFL 309

Query: 381 LRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLKLTPWIRNFLTRSLAIVP 440
           LRNV+GKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL+L PW+RNFLTR LAI+P
Sbjct: 310 LRNVVGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLRLEPWLRNFLTRCLAIIP 369

Query: 441 SLIVAIIGGSSGAGKLIIIAS--------------------------------ITVLTWI 500
           SLIVA+IGGS+GAGKLIIIAS                                I+ +TWI
Sbjct: 370 SLIVALIGGSAGAGKLIIIASMILSFELPFALVPLLKFTSSKTKMGSHANSLVISSVTWI 429

Query: 501 IGFLIMAINIYYLMSRFIHMLLHNDLHLAAVVFIGILGFSGVALYLAGIAYLVLRKSKE- 560
           IG LIM INIYYL+S FI +LLH+ ++L A+VF+G+LGFSG+A YLA I+YLVLRK++E 
Sbjct: 430 IGGLIMGINIYYLVSSFIKLLLHSHMNLVAIVFLGVLGFSGIATYLAAISYLVLRKNRES 489

Query: 561 -ITHLLALTTDESRRLSNEPSKTSGYSLPNEDIVSMQLPQRVRTANDVN 576
             TH L  +  ++             +LP EDI +MQLP RV    D+N
Sbjct: 490 SSTHFLDFSNSQTEE-----------TLPREDIANMQLPNRVAVIGDLN 527

BLAST of Clc10G12180 vs. TAIR 10
Match: AT1G80830.1 (natural resistance-associated macrophage protein 1 )

HSP 1 Score: 719.9 bits (1857), Expect = 1.6e-207
Identity = 388/538 (72.12%), Postives = 443/538 (82.34%), Query Frame = 0

Query: 68  SGSGQPQFILRA-GDESFSHAPLIENPETDQIVVPDKKSWKNLFAYMGPGFLVSIAYIDP 127
           +GSG+ QFI  + G+ SFS++PLIEN +++QI+V +KKSWKN FAY+GPGFLVSIAYIDP
Sbjct: 4   TGSGRSQFISSSGGNRSFSNSPLIENSDSNQIIVSEKKSWKNFFAYLGPGFLVSIAYIDP 63

Query: 128 GNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAANLGVVTGKHLAEHCKAEYPKAQNF 187
           GNFETDLQ+GA YKY LLWIILVAS AAL+IQSLAANLGVVTGKHLAE C+AEY K  NF
Sbjct: 64  GNFETDLQAGAHYKYELLWIILVASCAALVIQSLAANLGVVTGKHLAEQCRAEYSKVPNF 123

Query: 188 VLWILAEIAIVACDIPEVIGTAFALNMLFSIPVWCGVLLTGLSTLLLLALQQYGIRKLEF 247
           +LW++AEIA+VACDIPEVIGTAFALNMLFSIPVW GVLLTGLSTL+LLALQ+YG+RKLEF
Sbjct: 124 MLWVVAEIAVVACDIPEVIGTAFALNMLFSIPVWIGVLLTGLSTLILLALQKYGVRKLEF 183

Query: 248 LIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVPQLKGSGATGLAISLLGAMVMPHNLFL 307
           LIAFLV TIAICFF+EL Y+KPD GE+ +GLFVPQLKG+GATGLAISLLGAMVMPHNLFL
Sbjct: 184 LIAFLVFTIAICFFVELHYSKPDPGEVLHGLFVPQLKGNGATGLAISLLGAMVMPHNLFL 243

Query: 308 HSALVLSRKIPRSLSGIKEACRFYMIESGFALMVAFLINVSVISVSGAVCNAPDLNKEDQ 367
           HSALVLSRKIPRS SGIKEACRFY+IESG ALMVAFLINVSVISVSGAVCNAP+L+ ED+
Sbjct: 244 HSALVLSRKIPRSASGIKEACRFYLIESGLALMVAFLINVSVISVSGAVCNAPNLSPEDR 303

Query: 368 MSCNDLDLNKASFLLRNVLGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLKLTP 427
            +C DLDLNKASFLLRNV+GKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDL+L P
Sbjct: 304 ANCEDLDLNKASFLLRNVVGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLRLEP 363

Query: 428 WIRNFLTRSLAIVPSLIVAIIGGSSGAGKLIIIAS------------------------- 487
           W+RN LTR LAI+PSLIVA+IGGS+GAGKLIIIAS                         
Sbjct: 364 WLRNLLTRCLAIIPSLIVALIGGSAGAGKLIIIASMILSFELPFALVPLLKFTSCKTKMG 423

Query: 488 -------ITVLTWIIGFLIMAINIYYLMSRFIHMLLHNDLHLAAVVFIGILGFSGVALYL 547
                  IT LTW+IG LIM INIYYL+S FI +L+H+ + L  VVF GILGF+G+ALYL
Sbjct: 424 SHVNPMAITALTWVIGGLIMGINIYYLVSSFIKLLIHSHMKLILVVFCGILGFAGIALYL 483

Query: 548 AGIAYLVLRKSKEITHLLALTTDESRRLSNEPSKTSGYSLPNEDIVSMQLPQRVRTAN 573
           A IAYLV RK++  T LL         +S +       +LP +DIV+MQLP RV T++
Sbjct: 484 AAIAYLVFRKNRVATSLL---------ISRDSQNVE--TLPRQDIVNMQLPCRVSTSD 530

BLAST of Clc10G12180 vs. TAIR 10
Match: AT5G67330.1 (natural resistance associated macrophage protein 4 )

HSP 1 Score: 285.0 bits (728), Expect = 1.3e-76
Identity = 178/432 (41.20%), Postives = 263/432 (60.88%), Query Frame = 0

Query: 103 KKSWKNLFAYMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSLAA 162
           K SWK L+ + GPGFL+SIA++DPGN E+DLQ+GA   Y L+W+++ A+   L+IQ L+A
Sbjct: 46  KFSWKKLWLFTGPGFLMSIAFLDPGNLESDLQAGAIAGYSLIWLLMWATAIGLLIQLLSA 105

Query: 163 NLGVVTGKHLAEHCKAEYPKAQNFVLWILAEIAIVACDIPEVIGTAFALNMLFS--IPVW 222
            LGV TG+HLAE C+ EYP     VLWI+AEIA++  DI EVIG+A A+ +L +  +P+W
Sbjct: 106 RLGVATGRHLAELCREEYPTWARMVLWIMAEIALIGADIQEVIGSAIAIKILSNGLVPLW 165

Query: 223 CGVLLTGLSTLLLLALQQYGIRKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLFVP 282
            GV++T L   + L L+ YGIRKLE + A L+ T+A+ F    G  KP   E+  G  VP
Sbjct: 166 AGVVITALDCFIFLFLENYGIRKLEAVFAILIATMALAFAWMFGQTKPSGTELLVGALVP 225

Query: 283 QLKGSGATGLAISLLGAMVMPHNLFLHSALVLSRKI-PRSLSGIKEACRFYMIESGFALM 342
           +L  S     A+ ++G ++MPHN+FLHSALV SR++ P+    +KEA ++Y IES  AL 
Sbjct: 226 KL-SSRTIKQAVGIVGCIIMPHNVFLHSALVQSREVDPKKRFRVKEALKYYSIESTGALA 285

Query: 343 VAFLINVSVISVSGAVCNAPDLNKEDQMSCNDLDLNKASFLLRNVLGKWSSK---LFAIA 402
           V+F+INV V +V        ++        + + L  A   L++  G        ++AI 
Sbjct: 286 VSFIINVFVTTVFAKSFYGTEI-------ADTIGLANAGQYLQDKYGGGFFPILYIWAIG 345

Query: 403 LLASGQSSTITGTYAGQYVMQGFLDLKLTPWIRNFLTRSLAIVPSLIVAIIGGSSGA--- 462
           +LA+GQSSTITGTYAGQ++M GFL+LK+  W+R  +TRS AI+P++IVA++  SS +   
Sbjct: 346 VLAAGQSSTITGTYAGQFIMGGFLNLKMKKWVRALITRSCAIIPTMIVALVFDSSDSMLD 405

Query: 463 ---GKLIIIASITVLTWIIGFLIMAINIYYLMSRFIHMLLHNDLHLAAVVFIGILGFSGV 522
                L ++ S+ +   +I  L +  N   + S  I  L+     + A + I I G+  V
Sbjct: 406 ELNEWLNVLQSVQIPFAVIPLLCLVSNEQIMGSFKIQPLVQTISWIVAALVIAINGYLMV 465

BLAST of Clc10G12180 vs. TAIR 10
Match: AT4G18790.1 (NRAMP metal ion transporter family protein )

HSP 1 Score: 282.7 bits (722), Expect = 6.5e-76
Identity = 194/542 (35.79%), Postives = 290/542 (53.51%), Query Frame = 0

Query: 50  VSKREGKKERNNKMAAGGSGSGQPQFILRAGDESFSHAPLIENPETDQIVVPDKK----- 109
           VS++E   +R N        +G+ + +L           L E+P  +QI+  ++      
Sbjct: 6   VSRQENSPKRPN------DSNGEFKRLLVPETSQPEEDELHESPPENQILNVEEDRDKTY 65

Query: 110 ------SWKNLFAYMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQ 169
                 SW  L+ + GPGFL+SIA++DPGN E DLQ+GA   Y LLW++L A+   L++Q
Sbjct: 66  DSVPPFSWAKLWKFTGPGFLMSIAFLDPGNIEGDLQAGAVAGYSLLWLLLWATLMGLLMQ 125

Query: 170 SLAANLGVVTGKHLAEHCKAEYPKAQNFVLWILAEIAIVACDIPEVIGTAFALNMLFS-- 229
            L+A +GV TG+HLAE C++EYP     +LW +AE+A++  DI EVIG+A AL +L    
Sbjct: 126 LLSARIGVATGRHLAEICRSEYPSWARILLWFMAEVALIGADIQEVIGSAIALQILTRGF 185

Query: 230 IPVWCGVLLTGLSTLLLLALQQYGIRKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYG 289
           +P+W GV++T     L+  L++ G+RKLE L A L+ T+A+ F       KP V E+F G
Sbjct: 186 LPIWVGVIITSFDCFLISYLEKCGMRKLEGLFAVLIATMALSFAWMFNETKPSVEELFIG 245

Query: 290 LFVPQLKGSGATGLAISLLGAMVMPHNLFLHSALVLSRKI-PRSLSGIKEACRFYMIESG 349
           + +P+L GS     A+ ++G ++ PHN+FLHSALV SRK  P+ ++ ++EA  +Y IES 
Sbjct: 246 IIIPKL-GSKTIREAVGVVGCVITPHNVFLHSALVQSRKTDPKEINRVQEALNYYTIESS 305

Query: 350 FALMVAFLINVSVISVSGAVCNAPDLNKEDQMSCNDLDLNKASFLLRNVLGKWSSK---L 409
            AL V+F+IN+ V +V                  + + L  A + L+   G        +
Sbjct: 306 AALFVSFMINLFVTAVFAK-------GFYGTKQADSIGLVNAGYYLQEKYGGGVFPILYI 365

Query: 410 FAIALLASGQSSTITGTYAGQYVMQGFLDLKLTPWIRNFLTRSLAIVPSLIVAIIGGSSG 469
           + I LLA+GQSSTITGTYAGQ++M+GFLDL++  W+  F+TRS AIVP++ VAI+  +S 
Sbjct: 366 WGIGLLAAGQSSTITGTYAGQFIMEGFLDLQMEQWLSAFITRSFAIVPTMFVAIMFNTSE 425

Query: 470 A----------------------------------GKLIIIASITVLTWIIGFLIMAINI 529
                                              G   I  S+  L W +   +M IN 
Sbjct: 426 GSLDVLNEWLNILQSMQIPFAVIPLLTMVSNEHIMGVFKIGPSLEKLAWTVAVFVMMING 485

Query: 530 YYLMSRFIHMLLHNDLHLAAV--VFIGILGFSGVALYLAGIAYLVLRKSKEITHLLALTT 539
           Y L+          D  +A V    +G L F GV  Y++ I YLV  +S + +   +L  
Sbjct: 486 YLLL----------DFFMAEVEGFLVGFLVFGGVVGYISFIIYLVSYRSSQSSSWSSLEM 523

BLAST of Clc10G12180 vs. TAIR 10
Match: AT1G47240.1 (NRAMP metal ion transporter 2 )

HSP 1 Score: 278.1 bits (710), Expect = 1.6e-74
Identity = 170/426 (39.91%), Postives = 250/426 (58.69%), Query Frame = 0

Query: 101 PDKKSWKNLFAYMGPGFLVSIAYIDPGNFETDLQSGAQYKYGLLWIILVASFAALIIQSL 160
           P   SW+ L+ + GPGFL+SIA++DPGN E DLQ+GA   Y LLW+++ A+   L+IQ L
Sbjct: 60  PPPFSWRKLWLFTGPGFLMSIAFLDPGNLEGDLQAGAIAGYSLLWLLMWATAMGLLIQML 119

Query: 161 AANLGVVTGKHLAEHCKAEYPKAQNFVLWILAEIAIVACDIPEVIGTAFALNMLFS--IP 220
           +A +GV TG+HLAE C+ EYP    +VLW +AE+A++  DI EVIG+A A+ +L    +P
Sbjct: 120 SARVGVATGRHLAELCRDEYPTWARYVLWSMAELALIGADIQEVIGSAIAIQILSRGFLP 179

Query: 221 VWCGVLLTGLSTLLLLALQQYGIRKLEFLIAFLVLTIAICFFLELGYAKPDVGEIFYGLF 280
           +W GV++T     L L L+ YG+RKLE + A L+ T+ + F    G  KP   E+  G+ 
Sbjct: 180 LWAGVVITASDCFLFLFLENYGVRKLEAVFAVLIATMGLSFAWMFGETKPSGKELMIGIL 239

Query: 281 VPQLKGSGATGLAISLLGAMVMPHNLFLHSALVLSRKI-PRSLSGIKEACRFYMIESGFA 340
           +P+L  S     A+ ++G ++MPHN+FLHSALV SRKI P+  S ++EA  +Y+IES  A
Sbjct: 240 LPRL-SSKTIRQAVGVVGCVIMPHNVFLHSALVQSRKIDPKRKSRVQEALNYYLIESSVA 299

Query: 341 LMVAFLINVSVISVSGAVCNAPDLNKEDQMSCNDLDLNKASFLLRNVLGKWSSK---LFA 400
           L ++F+IN+ V +V        +         N++ L  A   L+   G        ++ 
Sbjct: 300 LFISFMINLFVTTVFAKGFYGTE-------KANNIGLVNAGQYLQEKFGGGLLPILYIWG 359

Query: 401 IALLASGQSSTITGTYAGQYVMQGFLDLKLTPWIRNFLTRSLAIVPSLIVAIIGGSSGAG 460
           I LLA+GQSSTITGTYAGQ++M GFL+L+L  W+R  +TRS AIVP++IVAI+  +S A 
Sbjct: 360 IGLLAAGQSSTITGTYAGQFIMGGFLNLRLKKWMRAVITRSCAIVPTMIVAIVFNTSEAS 419

Query: 461 K------LIIIASITV----------------------------LTWIIGFLIMAINIYY 487
                  L ++ S+ +                            + W +  L+M IN Y 
Sbjct: 420 LDVLNEWLNVLQSVQIPFALLPLLTLVSKEEIMGDFKIGPILQRIAWTVAALVMIINGYL 477

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYK27020.18.3e-27590.65metal transporter Nramp6 [Cucumis melo var. makuwa][more]
KAA0037704.18.0e-27091.01metal transporter Nramp6 [Cucumis melo var. makuwa][more]
XP_038905496.14.0e-26990.83metal transporter Nramp6-like [Benincasa hispida] >XP_038905497.1 metal transpor... [more]
XP_011651819.21.1e-26690.46metal transporter Nramp6 [Cucumis sativus] >XP_031739044.1 metal transporter Nra... [more]
KAA0048292.12.1e-26288.81metal transporter Nramp6 [Cucumis melo var. makuwa] >TYK14176.1 metal transporte... [more]
Match NameE-valueIdentityDescription
Q9S9N81.3e-20672.40Metal transporter Nramp6 OS=Arabidopsis thaliana OX=3702 GN=NRAMP6 PE=2 SV=2[more]
Q9SAH82.2e-20672.12Metal transporter Nramp1 OS=Arabidopsis thaliana OX=3702 GN=NRAMP1 PE=1 SV=1[more]
Q653V64.0e-19567.71Metal transporter Nramp3 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP3 PE=2... [more]
Q8H4H57.8e-14352.40Metal transporter Nramp5 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP5 PE=2... [more]
Q0D7E43.6e-14057.52Metal transporter Nramp1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP1 PE=2... [more]
Match NameE-valueIdentityDescription
A0A5D3DUP04.0e-27590.65Metal transporter Nramp6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A5A7T2K93.9e-27091.01Metal transporter Nramp6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
A0A0A0LD686.2e-26882.87Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G714870 PE=3 SV=1[more]
A0A5A7TXN61.0e-26288.81Metal transporter Nramp6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A6J1F4P33.4e-25887.66metal transporter Nramp6-like OS=Cucurbita moschata OX=3662 GN=LOC111442317 PE=3... [more]
Match NameE-valueIdentityDescription
AT1G15960.19.3e-20872.40NRAMP metal ion transporter 6 [more]
AT1G80830.11.6e-20772.12natural resistance-associated macrophage protein 1 [more]
AT5G67330.11.3e-7641.20natural resistance associated macrophage protein 4 [more]
AT4G18790.16.5e-7635.79NRAMP metal ion transporter family protein [more]
AT1G47240.11.6e-7439.91NRAMP metal ion transporter 2 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001046NRAMP familyPRINTSPR00447NATRESASSCMPcoord: 429..446
score: 53.78
coord: 291..314
score: 63.0
coord: 241..262
score: 42.18
coord: 188..214
score: 56.74
coord: 216..235
score: 42.6
coord: 401..420
score: 77.8
IPR001046NRAMP familyTIGRFAMTIGR01197TIGR01197coord: 108..476
e-value: 5.5E-114
score: 379.4
IPR001046NRAMP familyPFAMPF01566Nrampcoord: 129..476
e-value: 3.3E-108
score: 361.9
IPR001046NRAMP familyPANTHERPTHR11706SOLUTE CARRIER PROTEIN FAMILY 11 MEMBERcoord: 70..462
IPR001046NRAMP familyPANTHERPTHR11706SOLUTE CARRIER PROTEIN FAMILY 11 MEMBERcoord: 461..571
IPR001046NRAMP familyHAMAPMF_00221NRAMPcoord: 94..485
score: 35.587967
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..32
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR11706:SF85METAL TRANSPORTER NRAMP1coord: 70..462
NoneNo IPR availablePANTHERPTHR11706:SF85METAL TRANSPORTER NRAMP1coord: 461..571

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Clc10G12180.2Clc10G12180.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070574 cadmium ion transmembrane transport
biological_process GO:0071421 manganese ion transmembrane transport
biological_process GO:0030001 metal ion transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015086 cadmium ion transmembrane transporter activity
molecular_function GO:0005384 manganese ion transmembrane transporter activity
molecular_function GO:0046873 metal ion transmembrane transporter activity