Clc10G00880 (gene) Watermelon (cordophanus) v2

BLAST of Clc10G00880 vs. NCBI nr
Match: XP_038876564.1 (beta-carotene isomerase D27, chloroplastic [Benincasa hispida])

HSP 1 Score: 416.8 bits (1070), Expect = 1.8e-112
Identity = 226/326 (69.33%), Postives = 232/326 (71.17%), Query Frame = 0

Query: 3   LQSIQFFAAPPKEIRSRKKQRRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMTLFARK 62
           LQSIQFF APPKEIR RKKQ RF+RCGIAEASGEPAPLGQKTKYNDGPFEKVFMTLFARK
Sbjct: 6   LQSIQFFTAPPKEIRIRKKQSRFVRCGIAEASGEPAPLGQKTKYNDGPFEKVFMTLFARK 65

Query: 63  MEKFANAKEQRKEKVGLWDFLYDYERFVDVSKRVMQGKNRMQQQLIVREVLLSMLPPGAP 122
           MEKFAN KEQ K+KVGLWDFLYDYERFVDVSKRVMQGKNRMQQQL+VREVLLSMLPPGAP
Sbjct: 66  MEKFANTKEQGKKKVGLWDFLYDYERFVDVSKRVMQGKNRMQQQLVVREVLLSMLPPGAP 125

Query: 123 AQVSLSCKIKLFHVVVLLVTVMHICLLCQYEHSSTKIKLLLSNLRLAKKKKGKKKHILGI 182
           AQ       KLF               C++  S T                         
Sbjct: 126 AQFR-----KLFPPTK---------WACEFNASIT------------------------- 185

Query: 183 ICIFLRSSCGLKQKLMLAVQEIVPTNKMELRIQCLTNSAILSMVVEVEVNGIKQRSGVHI 242
                                 VP        Q L      S VVEVEVNGIKQRSGVHI
Sbjct: 186 ----------------------VPF------FQWLVGP---SEVVEVEVNGIKQRSGVHI 245

Query: 243 KKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEED 302
           KKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEED
Sbjct: 246 KKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEED 261

Query: 303 PVSNQPCYKDICSMANPSTPLCPKLQ 329
           PVSNQPCYKDICSMAN STP+CPKLQ
Sbjct: 306 PVSNQPCYKDICSMANSSTPVCPKLQ 261

BLAST of Clc10G00880 vs. NCBI nr
Match: XP_008448557.1 (PREDICTED: beta-carotene isomerase D27, chloroplastic [Cucumis melo] >KAA0052966.1 beta-carotene isomerase D27 [Cucumis melo var. makuwa] >TYK11422.1 beta-carotene isomerase D27 [Cucumis melo var. makuwa])

HSP 1 Score: 405.2 bits (1040), Expect = 5.3e-109
Identity = 223/328 (67.99%), Postives = 230/328 (70.12%), Query Frame = 0

Query: 3   LQSIQFFAAPPKEIRSRK-KQRRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMTLFAR 62
           LQSIQFF APPKEIR+RK  + RFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMTLFAR
Sbjct: 6   LQSIQFFTAPPKEIRNRKISKSRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMTLFAR 65

Query: 63  KMEKFANAKEQRKEKVGLWDFLYDYERFVDVSKRVMQGKNRMQQQLIVREVLLSMLPPGA 122
           KMEKFANAKEQRK+K G WDFLYDYERFVDVSKRVMQGKNRMQQQL+VREVLLSMLPPGA
Sbjct: 66  KMEKFANAKEQRKKKEGFWDFLYDYERFVDVSKRVMQGKNRMQQQLVVREVLLSMLPPGA 125

Query: 123 PAQVSLSCKIKLFHVVVLLVTVMHICLLCQYEHSSTKIKLLLSNLRLAKKKKGKKKHILG 182
           PAQ       KLF               C++  S T                        
Sbjct: 126 PAQFR-----KLFPPTK---------WACEFNASIT------------------------ 185

Query: 183 IICIFLRSSCGLKQKLMLAVQEIVPTNKMELRIQCLTNSAILSMVVEVEVNGIKQRSGVH 242
                                  VP        Q L      S VVEVEVNGIKQRSGVH
Sbjct: 186 -----------------------VPF------FQWLVGP---SEVVEVEVNGIKQRSGVH 245

Query: 243 IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE 302
           IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE
Sbjct: 246 IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE 263

Query: 303 DPVSNQPCYKDICSMANPSTPLCPKLQA 330
           DPVS QPCYKDICSM+N S PLCPKL A
Sbjct: 306 DPVSKQPCYKDICSMSNTSAPLCPKLLA 263

BLAST of Clc10G00880 vs. NCBI nr
Match: XP_011650294.1 (beta-carotene isomerase D27, chloroplastic [Cucumis sativus] >KGN55666.1 hypothetical protein Csa_010048 [Cucumis sativus])

HSP 1 Score: 397.9 bits (1021), Expect = 8.5e-107
Identity = 221/328 (67.38%), Postives = 228/328 (69.51%), Query Frame = 0

Query: 3   LQSIQFFAAPPKEIRSRKKQRRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMTLFARK 62
           LQSIQFF APPKEIR+RK + RFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMTLFARK
Sbjct: 6   LQSIQFFTAPPKEIRNRKIKSRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMTLFARK 65

Query: 63  MEKFANAKEQRKEKVGL-WDFLYDYERFVDVSKRVMQGKNRMQQQLIVREVLLSMLPPGA 122
           MEKFANAKEQRK+K GL WDFLYDYERFVDVSKRVMQGK RMQQQ++VREVLLSMLPPGA
Sbjct: 66  MEKFANAKEQRKKKEGLWWDFLYDYERFVDVSKRVMQGKTRMQQQIVVREVLLSMLPPGA 125

Query: 123 PAQVSLSCKIKLFHVVVLLVTVMHICLLCQYEHSSTKIKLLLSNLRLAKKKKGKKKHILG 182
           PAQ       KLF               C++    T                        
Sbjct: 126 PAQFR-----KLFPPTK---------WACEFNALIT------------------------ 185

Query: 183 IICIFLRSSCGLKQKLMLAVQEIVPTNKMELRIQCLTNSAILSMVVEVEVNGIKQRSGVH 242
                                  VP        Q L      S VVEVEVNGIKQRSGVH
Sbjct: 186 -----------------------VPF------FQWLVGP---SEVVEVEVNGIKQRSGVH 245

Query: 243 IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE 302
           IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE
Sbjct: 246 IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE 263

Query: 303 DPVSNQPCYKDICSMANPSTPLCPKLQA 330
           DPVS QPCYKDICSMA  S PLCPKL A
Sbjct: 306 DPVSKQPCYKDICSMAITSAPLCPKLLA 263

BLAST of Clc10G00880 vs. NCBI nr
Match: XP_023540699.1 (beta-carotene isomerase D27, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 379.8 bits (974), Expect = 2.4e-101
Identity = 216/332 (65.06%), Postives = 225/332 (67.77%), Query Frame = 0

Query: 3   LQSIQFFAAPPKEIRSRK-----KQRRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMT 62
           LQS QFF A P EIRSRK     K  R IRCGIAEASGEPAPLGQKTKYNDGPFEKVFMT
Sbjct: 6   LQSAQFFTA-PCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMT 65

Query: 63  LFARKMEKFANAKEQRKEKVGLWDFLYDYERFVDVSKRVMQGKNRMQQQLIVREVLLSML 122
           LFA+KMEK+AN+KEQ K K+G WDFLYDYERFVD SKRVMQGKNRMQQQL+VREVLLSML
Sbjct: 66  LFAKKMEKYANSKEQTK-KIGWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLLSML 125

Query: 123 PPGAPAQVSLSCKIKLFHVVVLLVTVMHICLLCQYEHSSTKIKLLLSNLRLAKKKKGKKK 182
           PPGAPAQ       KLF               C++  S T                    
Sbjct: 126 PPGAPAQFR-----KLFPPTK---------WACEFNASIT-------------------- 185

Query: 183 HILGIICIFLRSSCGLKQKLMLAVQEIVPTNKMELRIQCLTNSAILSMVVEVEVNGIKQR 242
                                      VP        Q L      S VVEVEVNGIKQR
Sbjct: 186 ---------------------------VPF------FQWLVGP---SEVVEVEVNGIKQR 245

Query: 243 SGVHIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPP 302
           SGVHIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPP
Sbjct: 246 SGVHIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPP 265

Query: 303 PFEEDPVSNQPCYKDICSMANPSTPLCPKLQA 330
           PFEEDPVS QPCYKDICSM+NPS+ LCPKL A
Sbjct: 306 PFEEDPVSKQPCYKDICSMSNPSSSLCPKLLA 265

BLAST of Clc10G00880 vs. NCBI nr
Match: XP_022942554.1 (beta-carotene isomerase D27, chloroplastic [Cucurbita moschata])

HSP 1 Score: 377.5 bits (968), Expect = 1.2e-100
Identity = 214/332 (64.46%), Postives = 225/332 (67.77%), Query Frame = 0

Query: 3   LQSIQFFAAPPKEIRSRK-----KQRRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMT 62
           LQS QFF A P EIRSRK     K  R IRCGIAEASGEPAPLGQKTKYNDGPFEKVFMT
Sbjct: 6   LQSAQFFTA-PCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMT 65

Query: 63  LFARKMEKFANAKEQRKEKVGLWDFLYDYERFVDVSKRVMQGKNRMQQQLIVREVLLSML 122
           LFA+KME++AN+KEQ K K+G WDFLYDYERFVD SKRVMQGKNRMQQQL+VREVLLSML
Sbjct: 66  LFAKKMERYANSKEQMK-KIGWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLLSML 125

Query: 123 PPGAPAQVSLSCKIKLFHVVVLLVTVMHICLLCQYEHSSTKIKLLLSNLRLAKKKKGKKK 182
           PPGAPAQ       KLF               C++  S T                    
Sbjct: 126 PPGAPAQFR-----KLFPPTK---------WACEFNASIT-------------------- 185

Query: 183 HILGIICIFLRSSCGLKQKLMLAVQEIVPTNKMELRIQCLTNSAILSMVVEVEVNGIKQR 242
                                      VP        Q L      S VVEVEVNG+KQR
Sbjct: 186 ---------------------------VPF------FQWLVGP---SEVVEVEVNGMKQR 245

Query: 243 SGVHIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPP 302
           SGVHIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPP
Sbjct: 246 SGVHIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPP 265

Query: 303 PFEEDPVSNQPCYKDICSMANPSTPLCPKLQA 330
           PFEEDPVS QPCYKDICSM+NPS+ LCPKL A
Sbjct: 306 PFEEDPVSKQPCYKDICSMSNPSSSLCPKLLA 265

BLAST of Clc10G00880 vs. ExPASy Swiss-Prot
Match: R4HZ96 (Beta-carotene isomerase D27, chloroplastic OS=Medicago truncatula OX=3880 GN=D27 PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 6.9e-27
Identity = 53/98 (54.08%), Postives = 72/98 (73.47%), Query Frame = 0

Query: 224 SMVVEVEVNGIKQRSGVHIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNF 283
           S V E E+NG ++++ VHIKKCR+LE + CVGMC+N+CK+P+Q F  + FG+P+ M PNF
Sbjct: 151 SEVRESEINGRREKNIVHIKKCRFLEETNCVGMCINLCKMPSQLFIKDSFGMPVNMVPNF 210

Query: 284 EDMSCEMIYGQVPPPFEEDPVSNQPCYKDICSMANPST 322
           +DMSCEMI+GQ PP   +DP   QPCYK   +  N +T
Sbjct: 211 DDMSCEMIFGQEPPASTDDPALKQPCYKLCKAKKNHAT 248

BLAST of Clc10G00880 vs. ExPASy Swiss-Prot
Match: Q7XA78 (Beta-carotene isomerase D27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=D27 PE=1 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 3.2e-24
Identity = 47/88 (53.41%), Postives = 66/88 (75.00%), Query Frame = 0

Query: 224 SMVVEVEVNGIKQRSGVHIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNF 283
           S V E EVNG K++S V+I+KCR+LE S CVGMC ++CKIP+Q F  N  G+P+ M P+F
Sbjct: 165 SEVRETEVNGRKEKSVVYIEKCRFLEQSNCVGMCTHICKIPSQIFIKNSLGMPIYMEPDF 224

Query: 284 EDMSCEMIYGQVPPPFEEDPVSNQPCYK 312
            D+SC+M++G+ PP  E+DP   QPC++
Sbjct: 225 NDLSCKMMFGREPPEIEDDPAMKQPCFE 252

BLAST of Clc10G00880 vs. ExPASy Swiss-Prot
Match: C7AU21 (Beta-carotene isomerase D27, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=D27 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 5.5e-24
Identity = 49/89 (55.06%), Postives = 66/89 (74.16%), Query Frame = 0

Query: 226 VVEVEVNGIKQRSGVHIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFED 285
           V+E EV G K+++ V+I KCR+LE++ CVGMC N+CKIP Q F  +  G+ + M+PNFED
Sbjct: 180 VMESEVEGRKEKNVVYIPKCRFLESTNCVGMCTNLCKIPCQKFIQDSLGMKVYMSPNFED 239

Query: 286 MSCEMIYGQVPPPFEEDPVSNQPCYKDIC 315
           MSCEMI+GQ PP  E+DP   QPC++  C
Sbjct: 240 MSCEMIFGQQPP--EDDPALKQPCFRTKC 266

BLAST of Clc10G00880 vs. ExPASy TrEMBL
Match: A0A5A7UFH7 (Beta-carotene isomerase D27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00760 PE=4 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 2.6e-109
Identity = 223/328 (67.99%), Postives = 230/328 (70.12%), Query Frame = 0

Query: 3   LQSIQFFAAPPKEIRSRK-KQRRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMTLFAR 62
           LQSIQFF APPKEIR+RK  + RFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMTLFAR
Sbjct: 6   LQSIQFFTAPPKEIRNRKISKSRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMTLFAR 65

Query: 63  KMEKFANAKEQRKEKVGLWDFLYDYERFVDVSKRVMQGKNRMQQQLIVREVLLSMLPPGA 122
           KMEKFANAKEQRK+K G WDFLYDYERFVDVSKRVMQGKNRMQQQL+VREVLLSMLPPGA
Sbjct: 66  KMEKFANAKEQRKKKEGFWDFLYDYERFVDVSKRVMQGKNRMQQQLVVREVLLSMLPPGA 125

Query: 123 PAQVSLSCKIKLFHVVVLLVTVMHICLLCQYEHSSTKIKLLLSNLRLAKKKKGKKKHILG 182
           PAQ       KLF               C++  S T                        
Sbjct: 126 PAQFR-----KLFPPTK---------WACEFNASIT------------------------ 185

Query: 183 IICIFLRSSCGLKQKLMLAVQEIVPTNKMELRIQCLTNSAILSMVVEVEVNGIKQRSGVH 242
                                  VP        Q L      S VVEVEVNGIKQRSGVH
Sbjct: 186 -----------------------VPF------FQWLVGP---SEVVEVEVNGIKQRSGVH 245

Query: 243 IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE 302
           IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE
Sbjct: 246 IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE 263

Query: 303 DPVSNQPCYKDICSMANPSTPLCPKLQA 330
           DPVS QPCYKDICSM+N S PLCPKL A
Sbjct: 306 DPVSKQPCYKDICSMSNTSAPLCPKLLA 263

BLAST of Clc10G00880 vs. ExPASy TrEMBL
Match: A0A1S3BKU4 (beta-carotene isomerase D27, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103490700 PE=4 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 2.6e-109
Identity = 223/328 (67.99%), Postives = 230/328 (70.12%), Query Frame = 0

Query: 3   LQSIQFFAAPPKEIRSRK-KQRRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMTLFAR 62
           LQSIQFF APPKEIR+RK  + RFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMTLFAR
Sbjct: 6   LQSIQFFTAPPKEIRNRKISKSRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMTLFAR 65

Query: 63  KMEKFANAKEQRKEKVGLWDFLYDYERFVDVSKRVMQGKNRMQQQLIVREVLLSMLPPGA 122
           KMEKFANAKEQRK+K G WDFLYDYERFVDVSKRVMQGKNRMQQQL+VREVLLSMLPPGA
Sbjct: 66  KMEKFANAKEQRKKKEGFWDFLYDYERFVDVSKRVMQGKNRMQQQLVVREVLLSMLPPGA 125

Query: 123 PAQVSLSCKIKLFHVVVLLVTVMHICLLCQYEHSSTKIKLLLSNLRLAKKKKGKKKHILG 182
           PAQ       KLF               C++  S T                        
Sbjct: 126 PAQFR-----KLFPPTK---------WACEFNASIT------------------------ 185

Query: 183 IICIFLRSSCGLKQKLMLAVQEIVPTNKMELRIQCLTNSAILSMVVEVEVNGIKQRSGVH 242
                                  VP        Q L      S VVEVEVNGIKQRSGVH
Sbjct: 186 -----------------------VPF------FQWLVGP---SEVVEVEVNGIKQRSGVH 245

Query: 243 IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE 302
           IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE
Sbjct: 246 IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE 263

Query: 303 DPVSNQPCYKDICSMANPSTPLCPKLQA 330
           DPVS QPCYKDICSM+N S PLCPKL A
Sbjct: 306 DPVSKQPCYKDICSMSNTSAPLCPKLLA 263

BLAST of Clc10G00880 vs. ExPASy TrEMBL
Match: A0A0A0L682 (DUF4033 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002850 PE=4 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 4.1e-107
Identity = 221/328 (67.38%), Postives = 228/328 (69.51%), Query Frame = 0

Query: 3   LQSIQFFAAPPKEIRSRKKQRRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMTLFARK 62
           LQSIQFF APPKEIR+RK + RFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMTLFARK
Sbjct: 6   LQSIQFFTAPPKEIRNRKIKSRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMTLFARK 65

Query: 63  MEKFANAKEQRKEKVGL-WDFLYDYERFVDVSKRVMQGKNRMQQQLIVREVLLSMLPPGA 122
           MEKFANAKEQRK+K GL WDFLYDYERFVDVSKRVMQGK RMQQQ++VREVLLSMLPPGA
Sbjct: 66  MEKFANAKEQRKKKEGLWWDFLYDYERFVDVSKRVMQGKTRMQQQIVVREVLLSMLPPGA 125

Query: 123 PAQVSLSCKIKLFHVVVLLVTVMHICLLCQYEHSSTKIKLLLSNLRLAKKKKGKKKHILG 182
           PAQ       KLF               C++    T                        
Sbjct: 126 PAQFR-----KLFPPTK---------WACEFNALIT------------------------ 185

Query: 183 IICIFLRSSCGLKQKLMLAVQEIVPTNKMELRIQCLTNSAILSMVVEVEVNGIKQRSGVH 242
                                  VP        Q L      S VVEVEVNGIKQRSGVH
Sbjct: 186 -----------------------VPF------FQWLVGP---SEVVEVEVNGIKQRSGVH 245

Query: 243 IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE 302
           IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE
Sbjct: 246 IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE 263

Query: 303 DPVSNQPCYKDICSMANPSTPLCPKLQA 330
           DPVS QPCYKDICSMA  S PLCPKL A
Sbjct: 306 DPVSKQPCYKDICSMAITSAPLCPKLLA 263

BLAST of Clc10G00880 vs. ExPASy TrEMBL
Match: A0A6J1FRN3 (beta-carotene isomerase D27, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111447551 PE=4 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 5.8e-101
Identity = 214/332 (64.46%), Postives = 225/332 (67.77%), Query Frame = 0

Query: 3   LQSIQFFAAPPKEIRSRK-----KQRRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMT 62
           LQS QFF A P EIRSRK     K  R IRCGIAEASGEPAPLGQKTKYNDGPFEKVFMT
Sbjct: 6   LQSAQFFTA-PCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMT 65

Query: 63  LFARKMEKFANAKEQRKEKVGLWDFLYDYERFVDVSKRVMQGKNRMQQQLIVREVLLSML 122
           LFA+KME++AN+KEQ K K+G WDFLYDYERFVD SKRVMQGKNRMQQQL+VREVLLSML
Sbjct: 66  LFAKKMERYANSKEQMK-KIGWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLLSML 125

Query: 123 PPGAPAQVSLSCKIKLFHVVVLLVTVMHICLLCQYEHSSTKIKLLLSNLRLAKKKKGKKK 182
           PPGAPAQ       KLF               C++  S T                    
Sbjct: 126 PPGAPAQFR-----KLFPPTK---------WACEFNASIT-------------------- 185

Query: 183 HILGIICIFLRSSCGLKQKLMLAVQEIVPTNKMELRIQCLTNSAILSMVVEVEVNGIKQR 242
                                      VP        Q L      S VVEVEVNG+KQR
Sbjct: 186 ---------------------------VPF------FQWLVGP---SEVVEVEVNGMKQR 245

Query: 243 SGVHIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPP 302
           SGVHIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPP
Sbjct: 246 SGVHIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPP 265

Query: 303 PFEEDPVSNQPCYKDICSMANPSTPLCPKLQA 330
           PFEEDPVS QPCYKDICSM+NPS+ LCPKL A
Sbjct: 306 PFEEDPVSKQPCYKDICSMSNPSSSLCPKLLA 265

BLAST of Clc10G00880 vs. ExPASy TrEMBL
Match: A0A6J1L2P3 (beta-carotene isomerase D27, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111498565 PE=4 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 3.7e-100
Identity = 214/332 (64.46%), Postives = 224/332 (67.47%), Query Frame = 0

Query: 3   LQSIQFFAAPPKEIRSRK-----KQRRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMT 62
           LQS QFF A P EIRSRK     K  R IRCGIAEASGEPAPLGQKTKYNDGPFEKVFMT
Sbjct: 6   LQSAQFFTA-PCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMT 65

Query: 63  LFARKMEKFANAKEQRKEKVGLWDFLYDYERFVDVSKRVMQGKNRMQQQLIVREVLLSML 122
           LFA+KME++AN+KEQ K K+G WDFLYDYERFVD SKRVMQGKNRMQQQL+VREVLLSML
Sbjct: 66  LFAKKMERYANSKEQTK-KIGWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLLSML 125

Query: 123 PPGAPAQVSLSCKIKLFHVVVLLVTVMHICLLCQYEHSSTKIKLLLSNLRLAKKKKGKKK 182
           PPGAPAQ       KLF               C++  S T                    
Sbjct: 126 PPGAPAQFR-----KLFPPTK---------WACEFNASIT-------------------- 185

Query: 183 HILGIICIFLRSSCGLKQKLMLAVQEIVPTNKMELRIQCLTNSAILSMVVEVEVNGIKQR 242
                                      VP        Q L      S VVEVEVNGIKQR
Sbjct: 186 ---------------------------VPF------FQWLVGP---SEVVEVEVNGIKQR 245

Query: 243 SGVHIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPP 302
           SGVHIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPP
Sbjct: 246 SGVHIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPP 265

Query: 303 PFEEDPVSNQPCYKDICSMANPSTPLCPKLQA 330
            FEEDPVS QPCYKDICSM+NPS+ LCPKL A
Sbjct: 306 LFEEDPVSKQPCYKDICSMSNPSSSLCPKLLA 265

BLAST of Clc10G00880 vs. TAIR 10
Match: AT1G64680.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G03055.1); Has 146 Blast hits to 146 proteins in 26 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 125; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 295.4 bits (755), Expect = 5.5e-80
Identity = 161/308 (52.27%), Postives = 187/308 (60.71%), Query Frame = 0

Query: 22  QRRFIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMTLFARKMEKFANAKEQRKEKVGLWD 81
           +RR  RCGIAE SGEPAP+G KT+Y DG  E+VFM LFARKM+KF + K++  ++ G W+
Sbjct: 15  RRRSTRCGIAEPSGEPAPMGLKTRYEDGLVERVFMGLFARKMDKFGSKKKKDTKEKGFWE 74

Query: 82  FLYDYERFVDVSKRVMQGKNRMQQQLIVREVLLSMLPPGAPAQVSLSCKIKLFHVVVLLV 141
             YDYE FV+VSKRVMQG++R+QQQ  VREVLLSMLPPGAP Q                 
Sbjct: 75  --YDYESFVEVSKRVMQGRSRVQQQEAVREVLLSMLPPGAPEQFR--------------- 134

Query: 142 TVMHICLLCQYEHSSTKIKLLLSNLRLAKKKKGKKKHILGIICIFLRSSCGLKQKLMLAV 201
                                    +L    K   +    +   F     G  Q      
Sbjct: 135 -------------------------KLFPPTKWAAEFNAALTVPFFHWLVGPSQ------ 194

Query: 202 QEIVPTNKMELRIQCLTNSAILSMVVEVEVNGIKQRSGVHIKKCRYLENSGCVGMCVNMC 261
                                   V+EVEVNG+KQRSGV IKKCRYLENSGCVGMCVNMC
Sbjct: 195 ------------------------VIEVEVNGVKQRSGVRIKKCRYLENSGCVGMCVNMC 250

Query: 262 KIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVSNQPCYKDICSMANPST 321
           KIPTQDFFTNEFGLPLTMNPN+EDMSCEMIYGQ PP FEED  + QPC  DICSM+NPS+
Sbjct: 255 KIPTQDFFTNEFGLPLTMNPNYEDMSCEMIYGQAPPAFEEDVATKQPCLADICSMSNPSS 250

Query: 322 PLCPKLQA 330
           P+CPKL+A
Sbjct: 315 PICPKLEA 250

BLAST of Clc10G00880 vs. TAIR 10
Match: AT1G03055.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 143 Blast hits to 143 proteins in 26 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 122; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 114.0 bits (284), Expect = 2.3e-25
Identity = 47/88 (53.41%), Postives = 66/88 (75.00%), Query Frame = 0

Query: 224 SMVVEVEVNGIKQRSGVHIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNF 283
           S V E EVNG K++S V+I+KCR+LE S CVGMC ++CKIP+Q F  N  G+P+ M P+F
Sbjct: 165 SEVRETEVNGRKEKSVVYIEKCRFLEQSNCVGMCTHICKIPSQIFIKNSLGMPIYMEPDF 224

Query: 284 EDMSCEMIYGQVPPPFEEDPVSNQPCYK 312
            D+SC+M++G+ PP  E+DP   QPC++
Sbjct: 225 NDLSCKMMFGREPPEIEDDPAMKQPCFE 252

BLAST of Clc10G00880 vs. TAIR 10
Match: AT4G01995.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 99.4 bits (246), Expect = 5.8e-21
Identity = 44/86 (51.16%), Postives = 60/86 (69.77%), Query Frame = 0

Query: 232 NGIKQRSGVHIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMI 291
           NG    SGV ++KC+YLE S CVG+C+N CK+PTQ FF +  G+PL M PNF+D SC+  
Sbjct: 163 NGESWDSGVFVEKCQYLEESKCVGVCINTCKLPTQTFFKDYMGVPLVMEPNFKDYSCQFK 222

Query: 292 YGQVPPPFEEDPVSNQPCYKDICSMA 318
           +G  PP  E+D   N+PC+ + CS+A
Sbjct: 223 FGVAPP--EDDGNVNEPCF-ETCSIA 245